LOCUS       NM_016369               3355 bp    mRNA    linear   PRI 17-SEP-2024
DEFINITION  Homo sapiens claudin 18 (CLDN18), transcript variant 1, mRNA.
ACCESSION   NM_016369
VERSION     NM_016369.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3355)
  AUTHORS   Matsuishi,A., Nakajima,S., Saito,M., Saito,K., Fukai,S.,
            Tsumuraya,H., Kanoda,R., Kikuchi,T., Nirei,A., Kaneta,A.,
            Okayama,H., Mimura,K., Hanayama,H., Sakamoto,W., Momma,T., Saze,Z.
            and Kono,K.
  TITLE     The impact of CLDN18.2 expression on effector cells mediating
            antibody-dependent cellular cytotoxicity in gastric cancer
  JOURNAL   Sci Rep 14 (1), 17916 (2024)
   PUBMED   39095563
  REMARK    GeneRIF: The impact of CLDN18.2 expression on effector cells
            mediating antibody-dependent cellular cytotoxicity in gastric
            cancer.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3355)
  AUTHORS   Choi,E., Shin,J., Ryu,M.H., Kim,H.D. and Park,Y.S.
  TITLE     Heterogeneity of claudin 18.2 expression in metastatic gastric
            cancer
  JOURNAL   Sci Rep 14 (1), 17648 (2024)
   PUBMED   39085339
  REMARK    GeneRIF: Heterogeneity of claudin 18.2 expression in metastatic
            gastric cancer.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3355)
  AUTHORS   Wu,J., Chen,J., Zhao,Y., Yuan,M., Chen,X., He,X., Zhang,J., Shao,G.
            and Sun,Q.
  TITLE     Molecular SPECT/CT Profiling of Claudin18.2 Expression In Vivo:
            Implication for Patients with Gastric Cancer
  JOURNAL   Mol Pharm 21 (7), 3447-3458 (2024)
   PUBMED   38843446
  REMARK    GeneRIF: Molecular SPECT/CT Profiling of Claudin18.2 Expression In
            Vivo: Implication for Patients with Gastric Cancer.
REFERENCE   4  (bases 1 to 3355)
  AUTHORS   Ogawa,H., Abe,H., Yagi,K., Seto,Y. and Ushiku,T.
  TITLE     Claudin-18 status and its correlation with HER2 and PD-L1
            expression in gastric cancer with peritoneal dissemination
  JOURNAL   Gastric Cancer 27 (4), 802-810 (2024)
   PUBMED   38724721
  REMARK    GeneRIF: Claudin-18 status and its correlation with HER2 and PD-L1
            expression in gastric cancer with peritoneal dissemination.
REFERENCE   5  (bases 1 to 3355)
  AUTHORS   De Sanctis,F., Dusi,S., Caligola,S., Anselmi,C., Petrova,V.,
            Rossi,B., Angelini,G., Erdeljan,M., Woll,S., Schlitter,A.M.,
            Metzler,T., Steiger,K., Borok,Z., Bailey,P., Bauer,A., Halin,C.,
            Boschi,F., Giugno,R., Cane,S., Lawlor,R., Corbo,V., Scarpa,A.,
            Constantin,G., Ugel,S., Vascotto,F., Sahin,U., Tureci,O. and
            Bronte,V.
  TITLE     Expression of the membrane tetraspanin claudin 18 on cancer cells
            promotes T lymphocyte infiltration and antitumor immunity in
            pancreatic cancer
  JOURNAL   Immunity 57 (6), 1378-1393 (2024)
   PUBMED   38749447
  REMARK    GeneRIF: Expression of the membrane tetraspanin claudin 18 on
            cancer cells promotes T lymphocyte infiltration and antitumor
            immunity in pancreatic cancer.
REFERENCE   6  (bases 1 to 3355)
  AUTHORS   Tsukita,S. and Furuse,M.
  TITLE     Claudin-based barrier in simple and stratified cellular sheets
  JOURNAL   Curr Opin Cell Biol 14 (5), 531-536 (2002)
   PUBMED   12231346
  REMARK    Review article
REFERENCE   7  (bases 1 to 3355)
  AUTHORS   Niimi,T., Nagashima,K., Ward,J.M., Minoo,P., Zimonjic,D.B.,
            Popescu,N.C. and Kimura,S.
  TITLE     claudin-18, a novel downstream target gene for the T/EBP/NKX2.1
            homeodomain transcription factor, encodes lung- and
            stomach-specific isoforms through alternative splicing
  JOURNAL   Mol Cell Biol 21 (21), 7380-7390 (2001)
   PUBMED   11585919
REFERENCE   8  (bases 1 to 3355)
  AUTHORS   Tsukita,S., Furuse,M. and Itoh,M.
  TITLE     Multifunctional strands in tight junctions
  JOURNAL   Nat Rev Mol Cell Biol 2 (4), 285-293 (2001)
   PUBMED   11283726
  REMARK    Review article
REFERENCE   9  (bases 1 to 3355)
  AUTHORS   Heiskala,M., Peterson,P.A. and Yang,Y.
  TITLE     The roles of claudin superfamily proteins in paracellular transport
  JOURNAL   Traffic 2 (2), 93-98 (2001)
   PUBMED   11247307
  REMARK    Review article
REFERENCE   10 (bases 1 to 3355)
  AUTHORS   Kniesel,U. and Wolburg,H.
  TITLE     Tight junctions of the blood-brain barrier
  JOURNAL   Cell Mol Neurobiol 20 (1), 57-76 (2000)
   PUBMED   10690502
  REMARK    Review article
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AY358479.1, AK098474.1, BM785703.1 and AY102073.1.
            
            On Nov 23, 2018 this sequence version replaced NM_016369.3.
            
            Summary: This gene encodes a member of the claudin family. Claudins
            are integral membrane proteins and components of tight junction
            strands. Tight junction strands serve as a physical barrier to
            prevent solutes and water from passing freely through the
            paracellular space between epithelial or endothelial cell sheets,
            and also play critical roles in maintaining cell polarity and
            signal transductions. This gene is upregulated in patients with
            ulcerative colitis and highly overexpressed in infiltrating ductal
            adenocarcinomas. PKC/MAPK/AP-1 (protein kinase C/mitogen-activated
            protein kinase/activator protein-1) dependent pathway regulates the
            expression of this gene in gastric cells. Alternatively spliced
            transcript variants encoding different isoforms have been
            identified. [provided by RefSeq, Jun 2010].
            
            Transcript Variant: This variant (1) encodes isoform 1, also known
            as isoform A1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC146668.1, DRR138524.1308982.1
                                           [ECO:0000332]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000183605.10/ ENSP00000183605.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-281               AY358479.1         29-309
            282-1863            AK098474.1         274-1855
            1864-2306           BM785703.1         143-585
            2307-2867           AK098474.1         2297-2857
            2868-3355           AY102073.1         394-881
FEATURES             Location/Qualifiers
     source          1..3355
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3q22.3"
     gene            1..3355
                     /gene="CLDN18"
                     /gene_synonym="SFTA5; SFTPJ"
                     /note="claudin 18"
                     /db_xref="GeneID:51208"
                     /db_xref="HGNC:HGNC:2039"
                     /db_xref="MIM:609210"
     exon            1..281
                     /gene="CLDN18"
                     /gene_synonym="SFTA5; SFTPJ"
                     /inference="alignment:Splign:2.1.0"
     CDS             62..847
                     /gene="CLDN18"
                     /gene_synonym="SFTA5; SFTPJ"
                     /note="isoform 1 is encoded by transcript variant 1;
                     surfactant associated 5; surfactant associated protein J;
                     surfactant, pulmonary associated protein J"
                     /codon_start=1
                     /product="claudin-18 isoform 1"
                     /protein_id="NP_057453.1"
                     /db_xref="CCDS:CCDS3095.1"
                     /db_xref="GeneID:51208"
                     /db_xref="HGNC:HGNC:2039"
                     /db_xref="MIM:609210"
                     /translation="MSTTTCQVVAFLLSILGLAGCIAATGMDMWSTQDLYDNPVTSVF
                     QYEGLWRSCVRQSSGFTECRPYFTILGLPAMLQAVRALMIVGIVLGAIGLLVSIFALK
                     CIRIGSMEDSAKANMTLTSGIMFIVSGLCAIAGVSVFANMLVTNFWMSTANMYTGMGG
                     MVQTVQTRYTFGAALFVGWVAGGLTLIGGVMMCIACRGLAPEETNYKAVSYHASGHSV
                     AYKPGGFKASTGFGSNTKNKKIYDGGARTEDEVQSYPSKHDYV"
     exon            282..446
                     /gene="CLDN18"
                     /gene_synonym="SFTA5; SFTPJ"
                     /inference="alignment:Splign:2.1.0"
     exon            447..564
                     /gene="CLDN18"
                     /gene_synonym="SFTA5; SFTPJ"
                     /inference="alignment:Splign:2.1.0"
     exon            565..675
                     /gene="CLDN18"
                     /gene_synonym="SFTA5; SFTPJ"
                     /inference="alignment:Splign:2.1.0"
     exon            676..3355
                     /gene="CLDN18"
                     /gene_synonym="SFTA5; SFTPJ"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3333..3338
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CLDN18"
                     /gene_synonym="SFTA5; SFTPJ"
                     /note="hexamer: ATTAAA"
     polyA_site      3355
                     /gene="CLDN18"
                     /gene_synonym="SFTA5; SFTPJ"
                     /note="major polyA site"
ORIGIN      
        1 acaccttcgg cagcaggagg gcggcagctt ctcgcaggcg gcagggcggg cggccaggat
       61 catgtccacc accacatgcc aagtggtggc gttcctcctg tccatcctgg ggctggccgg
      121 ctgcatcgcg gccaccggga tggacatgtg gagcacccag gacctgtacg acaaccccgt
      181 cacctccgtg ttccagtacg aagggctctg gaggagctgc gtgaggcaga gttcaggctt
      241 caccgaatgc aggccctatt tcaccatcct gggacttcca gccatgctgc aggcagtgcg
      301 agccctgatg atcgtaggca tcgtcctggg tgccattggc ctcctggtat ccatctttgc
      361 cctgaaatgc atccgcattg gcagcatgga ggactctgcc aaagccaaca tgacactgac
      421 ctccgggatc atgttcattg tctcaggtct ttgtgcaatt gctggagtgt ctgtgtttgc
      481 caacatgctg gtgactaact tctggatgtc cacagctaac atgtacaccg gcatgggtgg
      541 gatggtgcag actgttcaga ccaggtacac atttggtgcg gctctgttcg tgggctgggt
      601 cgctggaggc ctcacactaa ttgggggtgt gatgatgtgc atcgcctgcc ggggcctggc
      661 accagaagaa accaactaca aagccgtttc ttatcatgcc tcaggccaca gtgttgccta
      721 caagcctgga ggcttcaagg ccagcactgg ctttgggtcc aacaccaaaa acaagaagat
      781 atacgatgga ggtgcccgca cagaggacga ggtacaatct tatccttcca agcacgacta
      841 tgtgtaatgc tctaagacct ctcagcacgg gcggaagaaa ctcccggaga gctcacccaa
      901 aaaacaagga gatcccatct agatttcttc ttgcttttga ctcacagctg gaagttagaa
      961 aagcctcgat ttcatctttg gagaggccaa atggtcttag cctcagtctc tgtctctaaa
     1021 tattccacca taaaacagct gagttattta tgaattagag gctatagctc acattttcaa
     1081 tcctctattt ctttttttaa atataacttt ctactctgat gagagaatgt ggttttaatc
     1141 tctctctcac attttgatga tttagacaga ctccccctct tcctcctagt caataaaccc
     1201 attgatgatc tatttcccag cttatcccca agaaaacttt tgaaaggaaa gagtagaccc
     1261 aaagatgtta ttttctgctg tttgaatttt gtctccccac ccccaacttg gctagtaata
     1321 aacacttact gaagaagaag caataagaga aagatatttg taatctctcc agcccatgat
     1381 ctcggttttc ttacactgtg atcttaaaag ttaccaaacc aaagtcattt tcagtttgag
     1441 gcaaccaaac ctttctactg ctgttgacat cttcttatta cagcaacacc attctaggag
     1501 tttcctgagc tctccactgg agtcctcttt ctgtcgcggg tcagaaattg tccctagatg
     1561 aatgagaaaa ttattttttt taatttaagt cctaaatata gttaaaataa ataatgtttt
     1621 agtaaaatga tacactatct ctgtgaaata gcctcacccc tacatgtgga tagaaggaaa
     1681 tgaaaaaata attgctttga cattgtctat atggtacttt gtaaagtcat gcttaagtac
     1741 aaattccatg aaaagctcac tgatcctaat tctttccctt tgaggtctct atggctctga
     1801 ttgtacatga tagtaagtgt aagccatgta aaaagtaaat aatgtctggg cacagtggct
     1861 cacgcctgta atcctagcac tttgggaggc tgaggaggaa ggatcacttg agcccagaag
     1921 ttcgagacta gcctgggcaa catggagaag ccctgtctct acaaaataca gagagaaaaa
     1981 atcagccagt catggtggcc tacacctgta gtcccagcat tccgggaggc tgaggtggga
     2041 ggatcacttg agcccaggga ggttggggct gcagtgagcc atgatcacac cactgcactc
     2101 cagccaggtg acatagcgag atcctgtcta aaaaaataaa aaataaataa tggaacacag
     2161 caagtcctag gaagtaggtt aaaactaatt ctttaaaaaa aaaaaaaagt tgagcctgaa
     2221 ttaaatgtaa tgtttccaag tgacaggtat ccacatttgc atggttacaa gccactgcca
     2281 gttagcagta gcactttcct ggcactgtgg tcggttttgt tttgttttgc tttgtttaga
     2341 gacggggtct cactttccag gctggcctca aactcctgca ctcaagcaat tcttctaccc
     2401 tggcctccca agtagctgga attacaggtg tgcgccatca caactagctg gtggtcagtt
     2461 ttgttactct gagagctgtt cacttctctg aattcaccta gagtggttgg accatcagat
     2521 gtttgggcaa aactgaaagc tctttgcaac cacacacctt ccctgagctt acatcactgc
     2581 ccttttgagc agaaagtcta aattccttcc aagacagtag aattccatcc cagtaccaaa
     2641 gccagatagg ccccctagga aactgaggta agagcagtct ctaaaaacta cccacagcag
     2701 cattggtgca ggggaacttg gccattaggt tattatttga gaggaaagtc ctcacatcaa
     2761 tagtacatat gaaagtgacc tccaagggga ttggtgaata ctcataagga tcttcaggct
     2821 gaacagacta tgtctgggga aagaacggat tatgccccat taaataacaa gttgtgttca
     2881 agagtcagag cagtgagctc agaggccctt ctcactgaga cagcaacatt taaaccaaac
     2941 cagaggaagt atttgtggaa ctcactgcct cagtttgggt aaaggatgag cagacaagtc
     3001 aactaaagaa aaaagaaaag caaggaggag ggttgagcaa tctagagcat ggagtttgtt
     3061 aagtgctctc tggatttgag ttgaagagca tccatttgag ttgaaggcca cagggcacaa
     3121 tgagctctcc cttctaccac cagaaagtcc ctggtcaggt ctcaggtagt gcggtgtggc
     3181 tcagctgggt ttttaattag cgcattctct atccaacatt taattgtttg aaagcctcca
     3241 tatagttaga ttgtgctttg taattttgtt gttgttgctc tatcttattg tatatgcatt
     3301 gagtattaac ctgaatgttt tgttacttaa atattaaaaa cactgttatc ctaca
//
