LOCUS       NM_001185072            3533 bp    mRNA    linear   PRI 03-OCT-2024
DEFINITION  Homo sapiens claudin 12 (CLDN12), transcript variant 1, mRNA.
ACCESSION   NM_001185072
VERSION     NM_001185072.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3533)
  AUTHORS   Kolchakova,D., Moten,D., Batsalova,T. and Dzhambazov,B.
  TITLE     Tight Junction Protein Claudin-12 Is Involved in Cell Migration
            during Metastasis
  JOURNAL   Biomolecules 11 (5), 636 (2021)
   PUBMED   33922921
  REMARK    GeneRIF: Tight Junction Protein Claudin-12 Is Involved in Cell
            Migration during Metastasis.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3533)
  AUTHORS   Rahman,A., Kobayashi,M., Sugimoto,K., Endo,Y., Kojima,M.,
            Furukawa,S., Watanabe,T., Soeda,S., Hashimoto,Y., Fujimori,K. and
            Chiba,H.
  TITLE     Reduced Claudin-12 Expression Predicts Poor Prognosis in Cervical
            Cancer
  JOURNAL   Int J Mol Sci 22 (7), 3774 (2021)
   PUBMED   33917356
  REMARK    GeneRIF: Reduced Claudin-12 Expression Predicts Poor Prognosis in
            Cervical Cancer.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3533)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 3533)
  AUTHORS   Huang,J., Yin,P. and Zhang,L.
  TITLE     COPII cargo claudin-12 promotes hepatitis C virus entry
  JOURNAL   J Viral Hepat 26 (2), 308-312 (2019)
   PUBMED   30339745
  REMARK    GeneRIF: We found that COPII cargo CLDN12 is important for
            Hepatitis C Virus entry.
REFERENCE   5  (bases 1 to 3533)
  AUTHORS   Sweeney,M.D., Zhao,Z., Montagne,A., Nelson,A.R. and Zlokovic,B.V.
  TITLE     Blood-Brain Barrier: From Physiology to Disease and Back
  JOURNAL   Physiol Rev 99 (1), 21-78 (2019)
   PUBMED   30280653
  REMARK    Review article
REFERENCE   6  (bases 1 to 3533)
  AUTHORS   Kiuchi-Saishin,Y., Gotoh,S., Furuse,M., Takasuga,A., Tano,Y. and
            Tsukita,S.
  TITLE     Differential expression patterns of claudins, tight junction
            membrane proteins, in mouse nephron segments
  JOURNAL   J Am Soc Nephrol 13 (4), 875-886 (2002)
   PUBMED   11912246
REFERENCE   7  (bases 1 to 3533)
  AUTHORS   Niimi,T., Nagashima,K., Ward,J.M., Minoo,P., Zimonjic,D.B.,
            Popescu,N.C. and Kimura,S.
  TITLE     claudin-18, a novel downstream target gene for the T/EBP/NKX2.1
            homeodomain transcription factor, encodes lung- and
            stomach-specific isoforms through alternative splicing
  JOURNAL   Mol Cell Biol 21 (21), 7380-7390 (2001)
   PUBMED   11585919
REFERENCE   8  (bases 1 to 3533)
  AUTHORS   Tsukita,S., Furuse,M. and Itoh,M.
  TITLE     Multifunctional strands in tight junctions
  JOURNAL   Nat Rev Mol Cell Biol 2 (4), 285-293 (2001)
   PUBMED   11283726
  REMARK    Review article
REFERENCE   9  (bases 1 to 3533)
  AUTHORS   Heiskala,M., Peterson,P.A. and Yang,Y.
  TITLE     The roles of claudin superfamily proteins in paracellular transport
  JOURNAL   Traffic 2 (2), 93-98 (2001)
   PUBMED   11247307
  REMARK    Review article
REFERENCE   10 (bases 1 to 3533)
  AUTHORS   Kniesel,U. and Wolburg,H.
  TITLE     Tight junctions of the blood-brain barrier
  JOURNAL   Cell Mol Neurobiol 20 (1), 57-76 (2000)
   PUBMED   10690502
  REMARK    Review article
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC006153.2.
            
            On Nov 24, 2018 this sequence version replaced NM_001185072.2.
            
            Summary: This gene encodes a member of the claudin family. Claudins
            are integral membrane proteins and components of tight junction
            strands. Tight junction strands serve as a physical barrier to
            prevent solutes and water from passing freely through the
            paracellular space between epithelial or endothelial cell sheets,
            and also play critical roles in maintaining cell polarity and
            signal transductions. This gene is expressed in the inner ear and
            bladder epithelium, and it is over-expressed in colorectal
            carcinomas. This protein and claudin 2 are critical for vitamin
            D-dependent Ca2+ absorption between enterocytes. Multiple
            alternatively spliced transcript variants encoding the same protein
            have been found.[provided by RefSeq, Sep 2011].
            
            Transcript Variant: This variant (1) is the longest transcript.
            Variants 1-3 encode the same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660805.253211.1,
                                           SRR1660809.15320.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA1968832
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000496677.6/ ENSP00000419053.1
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-89                AC006153.2         39720-39808
            90-179              AC006153.2         41778-41867
            180-222             AC006153.2         48266-48308
            223-3533            AC006153.2         48903-52213
FEATURES             Location/Qualifiers
     source          1..3533
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7q21.13"
     gene            1..3533
                     /gene="CLDN12"
                     /note="claudin 12"
                     /db_xref="GeneID:9069"
                     /db_xref="HGNC:HGNC:2034"
                     /db_xref="MIM:611232"
     exon            1..89
                     /gene="CLDN12"
                     /inference="alignment:Splign:2.1.0"
     exon            90..179
                     /gene="CLDN12"
                     /inference="alignment:Splign:2.1.0"
     exon            180..222
                     /gene="CLDN12"
                     /inference="alignment:Splign:2.1.0"
     exon            223..3533
                     /gene="CLDN12"
                     /inference="alignment:Splign:2.1.0"
     CDS             256..990
                     /gene="CLDN12"
                     /codon_start=1
                     /product="claudin-12"
                     /protein_id="NP_001172001.1"
                     /db_xref="CCDS:CCDS5618.1"
                     /db_xref="GeneID:9069"
                     /db_xref="HGNC:HGNC:2034"
                     /db_xref="MIM:611232"
                     /translation="MGCRDVHAATVLSFLCGIASVAGLFAGTLLPNWRKLRLITFNRN
                     EKNLTVYTGLWVKCARYDGSSDCLMYDTTWYSSVDQLDLRVLQFALPLSMLIAMGALL
                     LCLIGMCNTAFRSSVPNIKLAKCLVNSAGCHLVAGLLFFLAGTVSLSPSIWVIFYNIH
                     LNKKFEPVFSFDYAVYVTIASAGGLFMTSLILFIWYCTCKSLPSPFWQPLYSHPPSMH
                     TYSQPYSARSRLSAIEIDIPVVSHTT"
     regulatory      3512..3517
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CLDN12"
                     /note="hexamer: ATTAAA"
     polyA_site      3533
                     /gene="CLDN12"
                     /note="major polyA site"
ORIGIN      
        1 gggaacgcac ttcctgggac gctgagaggg agacgctcca agaggctcct cagtgtgggc
       61 gagtaaaatg ccctgcgtgt gagaagcagg ctcagattat tgctactctg tattcagatc
      121 ttcatgtgtc tcttctaagc tgaataaagc tgttttggaa ctgtcaggta cctcaaagca
      181 tgaagaaaac gaggcacagg gaagttctga atctggccca agtctgactg acagtactcc
      241 acaagcttgc ctgccatggg ctgtcgggat gtccacgcag ccacagtcct ttccttcctg
      301 tgtggaatcg cctcagtagc aggcctcttt gcagggactc tgcttcccaa ctggagaaaa
      361 ttacgattga tcacattcaa cagaaacgag aagaacctga ctgtttacac aggcctgtgg
      421 gtgaaatgtg cccggtatga cgggagcagt gactgcctga tgtacgacac tacttggtac
      481 tcatcagttg accagctgga cctgcgtgtc ctccagtttg ccctacccct cagcatgctg
      541 atcgccatgg gtgccctgct gctctgcctg attggaatgt gcaacactgc cttcaggtcc
      601 tcggtgccca acatcaaact ggccaagtgt ctggtcaata gtgcaggttg ccacctggtg
      661 gctgggctgc tatttttcct ggcaggtact gtgagcctct ccccatctat ctgggtcatc
      721 ttttataaca tccatctgaa caagaagttt gagccagtct tttcatttga ctatgcagtg
      781 tatgtcacta ttgctagtgc tgggggcctg tttatgactt cccttatact atttatttgg
      841 tattgtacat gcaaatcttt gccttctcct ttctggcaac cattgtactc ccatccaccc
      901 agtatgcata cttactcaca gccctattca gcacgctctc gcctctctgc cattgaaatt
      961 gacattccag tagtttcaca caccacttaa tggggaaata gttaattgtt aaagaaaact
     1021 tcttgtagcc tcacattccc cttgtgcaaa gagctctttt ggacctacat acattttcct
     1081 ttgtttttga ccaatcaatg aagccaaatt tatatgtcct agtagaatga agtgctgcta
     1141 gtttttatga gaagtatatt atattaaatg tgaatttttt aaattttgct tcttatactg
     1201 gaaggaattt tagccttcat attgatatct aattaattat ttaagtggaa gaggcctgca
     1261 tcacaattga ggtaatgtag agcaacatgt taaagaatga tggttagcag aagctgttgt
     1321 atacaatctt catgaaaatt tcagtgtgta tttttctttt tctataatac ctttaactgc
     1381 aaagaaaagg cagtttcaaa tataagaaat ttatttcagg taagggtaat attttaatag
     1441 tagtcaataa tctagcttaa ggctgtaact cttctatcgg ggctaattgt atgaataggt
     1501 gtcagtatgt tgaagattac tttcttttgt gactttcttc tacctcatgc cactgtttaa
     1561 aagtaaaacg tattttaacg atgttagaat aagactacca ttctaaatat cacctactta
     1621 tgaataacat gtaataattt ttaacattaa tgattccata aattgtatta ttgggattag
     1681 aatgtgcttt atgacaggtt agtgtttcct ctgaggcaga aaactctttt ttggagatat
     1741 cttccatcaa gcagtactcg tgcccatata caatctctta gtggctagga gaaataaata
     1801 aaagggccat aatggtttgt tctctttcag acataattta gtaggggaca agaagtctgt
     1861 tcttcagtga gtacactaga gatttactct ggtgactgcc ttttgagtta tgggtgaagt
     1921 aaggtatggc tttaccataa ccttgattca ttcacccttg attcatttct cgcccccgtc
     1981 actgattatt tccttgagca tatatctctg cctaacactt tagtaggtgc tatagaggat
     2041 acatgaaaag tatgagatct ggttccatcc agtaagacat tttaatagag aagatcaaaa
     2101 tgttacctgg cagttgggga ataatctgac ttcgttggca gttggcctta acttcttaat
     2161 cattgatcca ggaatatttc aaccagagac acaactttct ggcagacaga caaattgtac
     2221 aacaccaaca atatcctgga ccttgaaatt ctgtttactt cagtccattg tatcctttaa
     2281 ggcacctgtg ctagcctaga ttttgtaata acactgattt atgagaatgg acaaaagtgg
     2341 tagggaaatt gttccctctc cacttctgaa agtatgatga tgtattaagg atggaggagt
     2401 tattaaaaat gtctcttctg atgaggtaac aattagatga aaccatgtta aagctgagat
     2461 gaacacttag aaattcaggg atattgggtc tttagcctta tgaatttgag ctgcttattt
     2521 aattggtgta atttactaca tattagtact atattcgtaa ggatttttta ttaaccatta
     2581 cagattttac aaacagctag ttatatggta aacagattat tatgcctttt tgcaattctg
     2641 aatatgattc tagtatttgt gtagatgtat ttggtacttt ttcccctaat tccaacacta
     2701 gtttatatat atagcgaata aatctagttg tataaatttt taaatgccgt cagtagaaag
     2761 cacacaaggt tatgattttt ttaattactg gcttctgatt tctttcactt ctgatccttt
     2821 tcctttttct cagatgtagc tgagtcttga tcattttaag acaacgatgg gtagaatttt
     2881 gagattaatg ttaattttcc ctttttgtta atttcagtcc cctctcacta tgcttttgtc
     2941 cagaaggatc aagaattcta ccatcccttg ggtctttgtg tataaacaat gttaaataaa
     3001 ggtagactca gtctttaaga tattagacag tttttttagt ccatgggatt gtaaatataa
     3061 acattaactt tcctataaga atattttggc tttgtaatct atagcctcaa attggtattt
     3121 attatggatt cactagacaa acagctgttt ccttattgtc ttttttcttt agtgtttctg
     3181 atttgctatc agtagctgtt tttaaagccg tccaaggaaa ataattattt acagtttttg
     3241 aagtcacttt tgagccctca tcaagctctc attgtgatgg gagggatacc tttttgttgt
     3301 taaaagccta ttattgttaa aggcctttta tggaaaccaa cttggaaaac aaccttaaat
     3361 gtggatgtat cagatttggt ttatccagcc atgggagaga aaacaaacct aagtttactt
     3421 tacttgtaca tatacactac aatggatagt atatttgctg taaactacaa tgtaaaacct
     3481 caataaaagt gcgctgtact tcttaatgtt tattaaaaga tgtattttta caa
//
