LOCUS       NM_001127898            9863 bp    mRNA    linear   PRI 08-OCT-2024
DEFINITION  Homo sapiens chloride voltage-gated channel 5 (CLCN5), transcript
            variant 2, mRNA.
ACCESSION   NM_001127898
VERSION     NM_001127898.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9863)
  AUTHORS   Li,Q., Yang,Z., Zang,R., Liu,S., Yu,L., Wang,J., Wang,C., Wang,X.
            and Sun,S.
  TITLE     Clinical features and genetic analysis of 15 Chinese children with
            dent disease
  JOURNAL   Ren Fail 46 (1), 2349133 (2024)
   PUBMED   38726999
  REMARK    GeneRIF: Clinical features and genetic analysis of 15 Chinese
            children with dent disease.
REFERENCE   2  (bases 1 to 9863)
  AUTHORS   Wang,Y., Xu,L., Zhang,Y., Fu,H., Gao,L., Guan,Y., Gu,W., Sun,J.,
            Chen,X., Yang,F., Lai,E., Wang,J., Jin,Y., Kou,Z., Qiu,X., Mao,J.
            and Hu,L.
  TITLE     Dent disease 1-linked novel CLCN5 mutations result in aberrant
            location and reduced ion currents
  JOURNAL   Int J Biol Macromol 257 (Pt 2), 128564 (2024)
   PUBMED   38061527
  REMARK    GeneRIF: Dent disease 1-linked novel CLCN5 mutations result in
            aberrant location and reduced ion currents.
REFERENCE   3  (bases 1 to 9863)
  AUTHORS   Ceol,M., Gianesello,L., Trimarchi,H., Migliorini,A., Priante,G.,
            Radu,C.M., Naso,E., Angelini,A., Calo,L.A., Anglani,F. and Del
            Prete,D.
  TITLE     Human parietal epithelial cells (PECs) and proteinuria in lupus
            nephritis: a role for ClC-5, megalin, and cubilin?
  JOURNAL   J Nephrol 36 (9), 2499-2506 (2023)
   PUBMED   37594671
  REMARK    GeneRIF: Human parietal epithelial cells (PECs) and proteinuria in
            lupus nephritis: a role for ClC-5, megalin, and cubilin?
REFERENCE   4  (bases 1 to 9863)
  AUTHORS   Ludwig,M., Waldegger,S., Nuutinen,M., Bokenkamp,A., Reissinger,A.,
            Steckelbroeck,S. and Utsch,B.
  TITLE     Four additional CLCN5 exons encode a widely expressed novel long
            CLC-5 isoform but fail to explain Dent's phenotype in patients
            without mutations in the short variant
  JOURNAL   Kidney Blood Press Res 26 (3), 176-184 (2003)
   PUBMED   12886045
  REMARK    GeneRIF: Dent's disease phenotype may be explained by mutations
            that affect so far unknown regulating elements of the CLCN5 gene or
            another gene(s), probably encoding CLC-5 accessory protein(s)
REFERENCE   5  (bases 1 to 9863)
  AUTHORS   Fisher,S.E., van Bakel,I., Lloyd,S.E., Pearce,S.H., Thakker,R.V.
            and Craig,I.W.
  TITLE     Cloning and characterization of CLCN5, the human kidney chloride
            channel gene implicated in Dent disease (an X-linked hereditary
            nephrolithiasis)
  JOURNAL   Genomics 29 (3), 598-606 (1995)
   PUBMED   8575751
REFERENCE   6  (bases 1 to 9863)
  AUTHORS   Igarashi,T., Hayakawa,H., Shiraga,H., Kawato,H., Yan,K.,
            Kawaguchi,H., Yamanaka,T., Tsuchida,S. and Akagi,K.
  TITLE     Hypercalciuria and nephrocalcinosis in patients with idiopathic
            low-molecular-weight proteinuria in Japan: is the disease identical
            to Dent's disease in United Kingdom?
  JOURNAL   Nephron 69 (3), 242-247 (1995)
   PUBMED   7753256
REFERENCE   7  (bases 1 to 9863)
  AUTHORS   Fisher,S.E., Black,G.C., Lloyd,S.E., Hatchwell,E., Wrong,O.,
            Thakker,R.V. and Craig,I.W.
  TITLE     Isolation and partial characterization of a chloride channel gene
            which is expressed in kidney and is a candidate for Dent's disease
            (an X-linked hereditary nephrolithiasis)
  JOURNAL   Hum Mol Genet 3 (11), 2053-2059 (1994)
   PUBMED   7874126
REFERENCE   8  (bases 1 to 9863)
  AUTHORS   Pook,M.A., Wrong,O., Wooding,C., Norden,A.G., Feest,T.G. and
            Thakker,R.V.
  TITLE     Dent's disease, a renal Fanconi syndrome with nephrocalcinosis and
            kidney stones, is associated with a microdeletion involving DXS255
            and maps to Xp11.22
  JOURNAL   Hum Mol Genet 2 (12), 2129-2134 (1993)
   PUBMED   8111383
REFERENCE   9  (bases 1 to 9863)
  AUTHORS   Scheinman,S.J., Pook,M.A., Wooding,C., Pang,J.T., Frymoyer,P.A. and
            Thakker,R.V.
  TITLE     Mapping the gene causing X-linked recessive nephrolithiasis to
            Xp11.22 by linkage studies
  JOURNAL   J Clin Invest 91 (6), 2351-2357 (1993)
   PUBMED   8099916
REFERENCE   10 (bases 1 to 9863)
  AUTHORS   Lieske,J.C., Milliner,D.S., Beara-Lasic,L., Harris,P., Cogal,A. and
            Abrash,E.
  TITLE     Dent Disease
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   22876375
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC236430.4, DA652242.1,
            AK056560.1, AC231645.2, BC051691.1 and AK025562.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_001127898.3.
            
            Summary: This gene encodes a member of the ClC family of chloride
            ion channels and ion transporters. The encoded protein is primarily
            localized to endosomal membranes and may function to facilitate
            albumin uptake by the renal proximal tubule. Mutations in this gene
            have been found in Dent disease and renal tubular disorders
            complicated by nephrolithiasis. Alternatively spliced transcript
            variants have been found for this gene. [provided by RefSeq, Jan
            2013].
            
            Transcript Variant: This variant (2, also known as type 1) uses an
            alternate splice site in the 5' UTR, compared to variant 1. Both
            variants 1 and 2 encode the same isoform (a).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803611.66851.1, AK056560.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA1970526
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000376091.8/ ENSP00000365259.3
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-19                AC236430.4         129359-129377
            20-69               DA652242.1         1-50
            70-1219             AK056560.1         1-1150
            1220-1220           AC231645.2         129108-129108
            1221-2804           AK056560.1         1152-2735
            2805-6550           AC231645.2         139547-143292
            6551-7975           BC051691.1         479-1903
            7976-9863           AK025562.1         22-1909
FEATURES             Location/Qualifiers
     source          1..9863
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="X"
                     /map="Xp11.23"
     gene            1..9863
                     /gene="CLCN5"
                     /gene_synonym="ClC-5; CLC5; CLCK2; DENT1; DENTS; hCIC-K2;
                     NPHL1; NPHL2; XLRH; XRN"
                     /note="chloride voltage-gated channel 5"
                     /db_xref="GeneID:1184"
                     /db_xref="HGNC:HGNC:2023"
                     /db_xref="MIM:300008"
     exon            1..197
                     /gene="CLCN5"
                     /gene_synonym="ClC-5; CLC5; CLCK2; DENT1; DENTS; hCIC-K2;
                     NPHL1; NPHL2; XLRH; XRN"
                     /inference="alignment:Splign:2.1.0"
     exon            198..273
                     /gene="CLCN5"
                     /gene_synonym="ClC-5; CLC5; CLCK2; DENT1; DENTS; hCIC-K2;
                     NPHL1; NPHL2; XLRH; XRN"
                     /inference="alignment:Splign:2.1.0"
     exon            274..417
                     /gene="CLCN5"
                     /gene_synonym="ClC-5; CLC5; CLCK2; DENT1; DENTS; hCIC-K2;
                     NPHL1; NPHL2; XLRH; XRN"
                     /inference="alignment:Splign:2.1.0"
     CDS             402..2852
                     /gene="CLCN5"
                     /gene_synonym="ClC-5; CLC5; CLCK2; DENT1; DENTS; hCIC-K2;
                     NPHL1; NPHL2; XLRH; XRN"
                     /note="isoform a is encoded by transcript variant 2;
                     H(+)/Cl(-) exchange transporter 5; chloride transporter
                     ClC-5; voltage-gated chloride ion channel CLCN5; chloride
                     channel, voltage-sensitive 5"
                     /codon_start=1
                     /product="H(+)/Cl(-) exchange transporter 5 isoform a"
                     /protein_id="NP_001121370.1"
                     /db_xref="CCDS:CCDS48115.1"
                     /db_xref="GeneID:1184"
                     /db_xref="HGNC:HGNC:2023"
                     /db_xref="MIM:300008"
                     /translation="MAMWQGAMDNRGFQQGSFSSFQNSSSDEDLMDIPATAMDFSMRD
                     DVPPLDREVGEDKSYNGGGIGSSNRIMDFLEEPIPGVGTYDDFNTIDWVREKSRDRDR
                     HREITNKSKESTWALIHSVSDAFSGWLLMLLIGLLSGSLAGLIDISAHWMTDLKEGIC
                     TGGFWFNHEHCCWNSEHVTFEERDKCPEWNSWSQLIISTDEGAFAYIVNYFMYVLWAL
                     LFAFLAVSLVKVFAPYACGSGIPEIKTILSGFIIRGYLGKWTLVIKTITLVLAVSSGL
                     SLGKEGPLVHVACCCGNILCHCFNKYRKNEAKRREVLSAAAAAGVSVAFGAPIGGVLF
                     SLEEVSYYFPLKTLWRSFFAALVAAFTLRSINPFGNSRLVLFYVEFHTPWHLFELVPF
                     ILLGIFGGLWGALFIRTNIAWCRKRKTTQLGKYPVIEVLVVTAITAILAFPNEYTRMS
                     TSELISELFNDCGLLDSSKLCDYENRFNTSKGGELPDRPAGVGVYSAMWQLALTLILK
                     IVITIFTFGMKIPSGLFIPSMAVGAIAGRLLGVGMEQLAYYHQEWTVFNSWCSQGADC
                     ITPGLYAMVGAAACLGGVTRMTVSLVVIMFELTGGLEYIVPLMAAAMTSKWVADALGR
                     EGIYDAHIRLNGYPFLEAKEEFAHKTLAMDVMKPRRNDPLLTVLTQDSMTVEDVETII
                     SETTYSGFPVVVSRESQRLVGFVLRRDLIISIENARKKQDGVVSTSIIYFTEHSPPLP
                     PYTPPTLKLRNILDLSPFTVTDLTPMEIVVDIFRKLGLRQCLVTHNGRLLGIITKKDV
                     LKHIAQMANQDPDSILFN"
     exon            418..564
                     /gene="CLCN5"
                     /gene_synonym="ClC-5; CLC5; CLCK2; DENT1; DENTS; hCIC-K2;
                     NPHL1; NPHL2; XLRH; XRN"
                     /inference="alignment:Splign:2.1.0"
     exon            565..716
                     /gene="CLCN5"
                     /gene_synonym="ClC-5; CLC5; CLCK2; DENT1; DENTS; hCIC-K2;
                     NPHL1; NPHL2; XLRH; XRN"
                     /inference="alignment:Splign:2.1.0"
     exon            717..816
                     /gene="CLCN5"
                     /gene_synonym="ClC-5; CLC5; CLCK2; DENT1; DENTS; hCIC-K2;
                     NPHL1; NPHL2; XLRH; XRN"
                     /inference="alignment:Splign:2.1.0"
     exon            817..1004
                     /gene="CLCN5"
                     /gene_synonym="ClC-5; CLC5; CLCK2; DENT1; DENTS; hCIC-K2;
                     NPHL1; NPHL2; XLRH; XRN"
                     /inference="alignment:Splign:2.1.0"
     exon            1005..1127
                     /gene="CLCN5"
                     /gene_synonym="ClC-5; CLC5; CLCK2; DENT1; DENTS; hCIC-K2;
                     NPHL1; NPHL2; XLRH; XRN"
                     /inference="alignment:Splign:2.1.0"
     exon            1128..1334
                     /gene="CLCN5"
                     /gene_synonym="ClC-5; CLC5; CLCK2; DENT1; DENTS; hCIC-K2;
                     NPHL1; NPHL2; XLRH; XRN"
                     /inference="alignment:Splign:2.1.0"
     exon            1335..1415
                     /gene="CLCN5"
                     /gene_synonym="ClC-5; CLC5; CLCK2; DENT1; DENTS; hCIC-K2;
                     NPHL1; NPHL2; XLRH; XRN"
                     /inference="alignment:Splign:2.1.0"
     exon            1416..1958
                     /gene="CLCN5"
                     /gene_synonym="ClC-5; CLC5; CLCK2; DENT1; DENTS; hCIC-K2;
                     NPHL1; NPHL2; XLRH; XRN"
                     /inference="alignment:Splign:2.1.0"
     exon            1959..2145
                     /gene="CLCN5"
                     /gene_synonym="ClC-5; CLC5; CLCK2; DENT1; DENTS; hCIC-K2;
                     NPHL1; NPHL2; XLRH; XRN"
                     /inference="alignment:Splign:2.1.0"
     exon            2146..2544
                     /gene="CLCN5"
                     /gene_synonym="ClC-5; CLC5; CLCK2; DENT1; DENTS; hCIC-K2;
                     NPHL1; NPHL2; XLRH; XRN"
                     /inference="alignment:Splign:2.1.0"
     exon            2545..2761
                     /gene="CLCN5"
                     /gene_synonym="ClC-5; CLC5; CLCK2; DENT1; DENTS; hCIC-K2;
                     NPHL1; NPHL2; XLRH; XRN"
                     /inference="alignment:Splign:2.1.0"
     exon            2762..9863
                     /gene="CLCN5"
                     /gene_synonym="ClC-5; CLC5; CLCK2; DENT1; DENTS; hCIC-K2;
                     NPHL1; NPHL2; XLRH; XRN"
                     /inference="alignment:Splign:2.1.0"
     regulatory      9843..9848
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CLCN5"
                     /gene_synonym="ClC-5; CLC5; CLCK2; DENT1; DENTS; hCIC-K2;
                     NPHL1; NPHL2; XLRH; XRN"
                     /note="hexamer: AATAAA"
     polyA_site      9863
                     /gene="CLCN5"
                     /gene_synonym="ClC-5; CLC5; CLCK2; DENT1; DENTS; hCIC-K2;
                     NPHL1; NPHL2; XLRH; XRN"
                     /note="major polyA site"
ORIGIN      
        1 gatactcgcg aggcgccctg tcacatgagc cgcgcgcccg cttcgctccc cctcctcccc
       61 ggctgggctg tgtgaatgaa gctctgccgg ctacgtgggg cgctcactca acttggtgtc
      121 ctggacgcca gagccgaccg agcgcgctgc ccaccggcgg cggacacggg ctccgccgct
      181 ccggacctcg gcgacagagc aaatcagttg cctggagttc ccagtgaagt tgtacctgtg
      241 cccgtgcgat aacgtcagtg gaagctgccc cgagtttggg gctttagccc cttggagaaa
      301 acagggccac atagctgcct ttctatcaag tctccctaca aaactgagag gctctgggaa
      361 actcagcctg tgaccccagc gtgggtgaag ataggatcaa gatggccatg tggcagggtg
      421 ccatggataa cagaggcttt cagcagggga gttttagtag cttccagaac agctccagtg
      481 atgaagacct gatggacatt ccagcaaccg ctatggattt ctccatgaga gatgatgttc
      541 ctcccttaga ccgagaagta ggagaggaca agtcgtacaa tggtggagga ataggttctt
      601 caaataggat catggacttc ttggaggagc caatccctgg tgtagggacc tatgatgatt
      661 tcaatacaat tgattgggtg agagagaagt ctcgagaccg ggataggcac cgagagatta
      721 ccaataaaag caaagagtca acatgggcct taattcacag tgtgagtgat gctttttccg
      781 gctggttgtt gatgctcctt attgggcttt tatcaggttc gttagctggt ttgatagaca
      841 tctctgctca ttggatgaca gacttaaaag aaggtatatg cacaggggga ttctggttta
      901 accatgaaca ttgttgctgg aactctgagc atgtcacctt tgaagagaga gacaaatgtc
      961 cagagtggaa tagttggtcc cagcttatca tcagcacaga tgagggagcc tttgcctaca
     1021 tagtcaatta tttcatgtac gtcctctggg ctctcctatt tgccttcctt gccgtatctc
     1081 ttgtcaaggt gtttgcgcct tatgcctgtg gctctggaat ccctgagata aaaactatct
     1141 tgagtggttt cattattagg ggctatttgg gtaagtggac tctggttatc aaaaccatca
     1201 ccttggtgct ggcagtgtca tctggcttga gcctgggcaa agagggccct ctagtgcacg
     1261 tggcttgctg ctgtgggaac atcctgtgcc actgcttcaa caaatacagg aagaatgaag
     1321 ccaagcgcag agaggtcttg tcggctgcag cagcagctgg tgtatctgta gcctttggag
     1381 cacctatagg tggagtatta ttcagccttg aagaggtcag ctactatttt cccctcaaaa
     1441 cattgtggcg ttcattcttt gctgccttgg tggcagcatt cactctacgc tccatcaatc
     1501 catttgggaa cagccgcctg gtactatttt atgtggagtt tcacacccca tggcatctct
     1561 ttgagctcgt gccattcatt ctgctgggca tatttggtgg tctgtgggga gcactgttta
     1621 tccgcacaaa cattgcctgg tgtcggaagc gaaagaccac ccagttgggc aagtatcctg
     1681 ttatagaggt actcgtcgtg acagccatca ctgccatcct ggctttcccc aatgaataca
     1741 ctcggatgag cacaagtgag ctcatttctg agctgtttaa tgactgtggc cttctggact
     1801 cctccaagct ctgtgattat gagaaccgtt tcaacacaag caaagggggt gaactgcctg
     1861 acagaccggc tggcgtggga gtctacagtg caatgtggca gctggcttta acactcatac
     1921 tgaaaattgt cattactata ttcacctttg gcatgaagat cccttctggc ctctttatcc
     1981 ctagcatggc tgttggtgct atagcaggtc gacttctagg agtaggaatg gaacagctgg
     2041 cttattacca ccaggaatgg accgtcttca atagctggtg tagtcaggga gctgattgca
     2101 tcacccccgg cctttatgca atggttgggg ctgcagcctg cttaggtggg gtgactcgga
     2161 tgactgtttc tcttgttgtc ataatgtttg aactgactgg tggcttagaa tacatcgtgc
     2221 ctctgatggc tgcagccatg acaagcaagt gggtggcaga tgctcttggg cgggagggca
     2281 tctatgatgc ccacatccgt ctcaatggat acccctttct tgaagccaaa gaagagtttg
     2341 ctcataagac cctggcaatg gatgtgatga aaccccggag aaatgatcct ttgttgactg
     2401 tccttactca ggacagtatg actgtggaag atgtagagac cataatcagt gaaaccactt
     2461 acagtggctt cccagtggtg gtatcccggg agtcccaaag acttgtgggc tttgtcctcc
     2521 gaagagatct cattatttca attgaaaatg ctcgaaagaa acaggatggg gttgttagca
     2581 cttccatcat ttatttcacg gagcattctc ctccattgcc accatacact ccacccactc
     2641 taaagcttcg gaacatcctc gatctcagcc ccttcactgt gactgacctt acacccatgg
     2701 agatcgtagt ggatattttc cgaaagctgg gactgcggca gtgcctggtt acacacaacg
     2761 ggcgattgct tggaatcatt accaaaaagg atgtgttaaa gcatatagca cagatggcga
     2821 accaagatcc tgattccatt ctcttcaact agaatcatag agttctggat gtaaagcggg
     2881 aaggacatta cagaccatgg atatgttgta ttaactgggt acccaaaaca cattttccat
     2941 atttggatgg tgaagtcaca ttagtgtgtt gtctctttcc tacaagttaa ccagttgcac
     3001 tacataatct ctggaaatta attttctctt taggagaaat tatagttagg cttccatgat
     3061 gttacattag gaagatatca tgaaagaata aataagattg ctatggttta attatatttg
     3121 ctttttaaaa gattttttta acttaaaaag tagttagcca atatgcaatc actgaaaact
     3181 atgcaagaga aattccaacc gtcctgacct ataacctgta ggaaaccgac gaaaaagtca
     3241 ctcttttggg atctaactgt tgttactgga agacgaagtg taaactaagg ggctttgctt
     3301 ttcaaaccag agaaaggaaa gccagaagga aaagagtaat ggtattttct agactgtgaa
     3361 gattcagttc aaatgttatc cttgttcctg ttacaatatt tagcattatt agtttgttat
     3421 gtgtgtatgt ttatgttaat tttaatttct gattataaga caatgctgct ttggttaatc
     3481 tcttctaaat gaatttagag agattgactt ttatagcttg cttgttgctg gtactctttt
     3541 gaagtgcaca aagataagtt atagagcttt ctccttgtct gcaaaaacgg taattttcca
     3601 ggtggcattt gttagctggt agacaaggac tttgccgtga atttgttagt agcaaggaat
     3661 gatttcttcc agcttccttg aaatgaataa ttagtaaact tctaagcaaa gtcaatgact
     3721 aggagtttca catgtttgtg aggtcctaac taattctctt acccagatgc cacctccatg
     3781 aatgatggtg ctttaggctt ctggaatatg taaaaacagc aaagggaacc aagtctagac
     3841 tgcatactgg taaaccaggg aagactcaga actgcatatg catcctcatg cattcctttg
     3901 taaagaccac cagtactaaa ataactggac actcattgta cctcccagcg ataagtatgt
     3961 gtaacaggcc agtgtgttgt ccccatgatc atgaattagg cttgggatgc ttccaaatat
     4021 attcaaggtg ttggaacagg ataggcagct gtgactccct caagagtcct tagaattcta
     4081 aataaaatgc agagccatcc taagacacag gggtatggac aaggcctggt gacaccaaag
     4141 gtgcttgtat ccaattgaaa aggtgcctgt ctcaatttca taccaccttt attgcagtta
     4201 aaaaaaaatc acactctggg gacacttggt ggaattacag agccaccatc atcattccaa
     4261 ccactatttc attttctgta gttttacctg tgtgcaatta ctccccctcc attctgttcc
     4321 acttcttact ccttagtacc aaatcctctg tttgggattg agcgctgctc ctggttaatc
     4381 attcctacta caaaaaaaaa taactcccag ggctagttaa attgtaaacc aaggctcagc
     4441 agtctcacaa cacatggacc agaggtgaca cacagccatt tcctttgcca tgtggcccag
     4501 ttgctgctgc catgcctcca tttccacact ggatgcctac ggcagtgaga tttcactgcc
     4561 ggggtaagag ttcagcctgg atgattttat agctctgttc ctagcacttc tcatcatcct
     4621 tccagcccag aatcagcggt cattctgcat attcccacca accctctacc cccaaacact
     4681 tcagtgtacc tcattttaag agttgctgat ccctgattct aggacgtttt tacccatagt
     4741 tcttgtcttt ccaaaatctg aaattctttt ttttgctcag aactgggtag ccaagggtta
     4801 ttttattttt atctttaaaa taatcaaggc agtcgctaga gtttctcctt gtgaatagat
     4861 cactctagca ttttaatgaa aaagaaaaaa atctttctgg ggtatgttgt atcatagtaa
     4921 tggctcagta accacatatt ttgtcctttc catgtcactg attccttcat atgagactat
     4981 ttggcttgac taccctgtat attgtgtaga aatcaaagtt cttatctgta catttctggt
     5041 ccaatacctg tcttattagt tgtccttccc cactaaagtt tgcaaaacag aaaatgttac
     5101 tatttctggt atttaatgac aatgaaaggt ttggtcatat ttcatagtgc agtagcgata
     5161 aggagggtgt ctcaagtttg cttttgtgtt tcttctaggc tttgttcttg attttagaca
     5221 gatgagccag tgttaaggtg ctacttcaga gaataaatta gagaaatcag ataacactgt
     5281 gccaaggtat actttgtaga tgctaagcac cgattggccc tccagagaat gacaatatcc
     5341 ctttgatacc tcactcctga tcaatctcat tcattaacct cagcttctca ggaatccttg
     5401 tagcttgtat gctagaagta gctatggtat atggtatcca ctatatgtcc agtcctatgg
     5461 tttggctcaa aggttggcta attgcagcta gagctctcaa ctgctagctc agcaccttga
     5521 catttgaagt ttcttgtctt gaggagaagg aaaatgttct ttctgccatt gccactttaa
     5581 cagttggaca tgcttaagca acaaatacag ccagtatagg gaggactgtc agtagcatca
     5641 aatggccaca tacctcaatg gcaaaagact agatggtttg tgatttaaaa attaatgagt
     5701 cgagatgaga ctattttggt attaaatgag aaaattgcct aacacaaggg aagggtttac
     5761 aaaagaatgc caattagtgt ttcaacagag ttaggatttt ccaagtaaga gtagctggta
     5821 gccatccagc cataaaagga acgagagtct atagatttgt aaaagtaaaa ctgctatatt
     5881 ttgtatcttc aaatcttcaa tataaagaat ttaaagaacc tgaaacagtt aatgcatgat
     5941 tcgttatatt ggagggaaaa agcaggggac tgataataca aaataatggc aactttaaag
     6001 atcatttcta cgtggatgtt tggttagaaa catattccaa tgctctcctc tttctctggc
     6061 cacagcatcc tggctcagcc cagctgtgga gtagaggtgg taagatccac agaacagata
     6121 tcctctggta gataagctga cattgatgat actttgaatt taccagctgg tgaagcaaac
     6181 gattaatttg tgattgaact gaaaagcagt tgaattgttt tgacaccaga ggctgaatct
     6241 aaacccttta caggaagagc agagcatact atacatttct tagcagatct tcaattctgt
     6301 ttgaacatga tgaagtaacc agggtttagt gagatctaag gccgtgaagt aattcacaac
     6361 caagatctta tgaacgcatt cagatgtgag gggggcaaat atgttttcaa aaacttagtg
     6421 tgcaatagag tccattatag aaattaacca ctgctatatg ttttaagtag ttcaatcacc
     6481 tgttgtggtt cagtcttgat tattgtttat tttgttaggt gataagtagg aagatgcact
     6541 tgttatccca cactatctct tgttttgttt tgttttgttt ttaaataatg aacagtgcaa
     6601 tctttgaact tgagttttta ataatgatag caaagacaat ggtgagaact tgaaacagcc
     6661 agcatatttt atattgccaa agtacttact gatgactgac cacactgtct tgtgaggaac
     6721 tgggtcccct gcactggatc agagccaaat gcaagccacc catcagttca gaggaagcag
     6781 gaaaacatct tttgggtggt gcatagaaca ttgagctctt aaaaacccgg agaaaaatta
     6841 ggaatttaga aagtaagtgg gaggtagaat attgagtcct tttttaaaaa aaagaattga
     6901 tgtctaaata tgtagtctga acttaagtgt cctaaaacct tgttttttgt ttttttgttt
     6961 tttgaggagt cgtcttgctc tatcaccagg tgggagtgca gtggcacaat ctcggctcac
     7021 tgtaacctct gcctcctgcc tcccgggtcc aagcaattcc cctgcctcag tctcccaagt
     7081 aggtgggact acaggcgcac accaccatgc ccagctaact tttgtatttt tagtagagac
     7141 ggggtttcac catgttggcc aggatggtct cgatctcttg acctcgtgat ccacccatct
     7201 cggcctccca aagtgctggg attataggtg tgagccaccg cacccagcca aaccttgatt
     7261 tttttagcca cctctgggat tgcctgggtt ccctttggag aagaaactaa aagttatggc
     7321 catagaaaaa gccctggtaa gaaaatattt gaatggtgta agatggtgga aggaaagcca
     7381 tcccacaccc ctacaacata tgttctggca tttataaact gcttgattgt gtgcctcaag
     7441 gggagaaaaa gtcaagcaat gcttcaaagg ccaggctttc taagcaaatg agtggttcaa
     7501 agcagtgttt ggctcaaggg cagtctggtt ttatggatag ggccgaccca agacatctgc
     7561 tcatgaagaa atgtggcatc tgttgccatt tcctaaacta taaggtttag atagattgtc
     7621 ttgaattttg atagcaaatt tttttaaatt tatatttctg cctcctcgta catccttata
     7681 tatggaaaaa aaagtaagag ttcctcagat cagagctcag tagctgtgtc aatttgaagt
     7741 tcaatcagtg tagttgtcct ggggtgatcg ctgactctgc tctgtttcat atttcagttg
     7801 cttgctccaa ggagagaatt tctgagaaca atgggtgtta gcaactttaa gttactcccc
     7861 aaacgtttgc tggccacatt ctcatttcta aacatctgga tctgggtaaa ggtttgaata
     7921 gatgtaggga acaaaattgg tttttccatg gtgtagtcta attttgccaa atcacccaag
     7981 aaagaaagta tatgacgtgt ggagtttgca tttcttggat gggacctatc gggtcaccca
     8041 gcccagtctt acagtcataa attcctacct cttagttcct gccaaagggc cttcagcctc
     8101 tgtgtgcaca cttattgaat gaatgctttg cctaaagttt ggccttttga agaaaaacag
     8161 catgtgataa tataaaaata tgagagttta aaaaaaaaaa agatctctgc ctctcttctc
     8221 tctgcctcgt cccaccttgc tgtctgtctc attgtctttc tctcccctca ccatgcgctg
     8281 aattcagatt tctctttctt ttattatagg catgttgttt tgaatgtgga ctttgtggga
     8341 gggagaggat tgttctgaga acagaaactg gaagatacgt ctgagttgat gggaccaaca
     8401 gggctttgct cctctgtgag ggccaaagcc attgatactt gtcgggggtg atgagggctt
     8461 tagcatctca aggcagacat atgaagtatt tactgctaaa ctatattact tactagtaca
     8521 aatctgttgg aattcagggg cagtctaaga tttggtgagt gaaatgccat ctggcaaaat
     8581 cgagaagaga gtgtggcctg agaggggaat gttcagcctc actgtcacat taagggtacc
     8641 tcaaaataca tggtccttcc agtgtgttcc ttttccttcc tccatttcac tttgtcccct
     8701 cttcttcctt tctctcccat tttcctggct ttttagcact cagctttttt ttataagtat
     8761 gtatttactt tttattatat atttggtcct ttgggacttt gtgatgatta agaatttatt
     8821 ccattcagga ttttaaaatg atgtgtttaa tattttgttc tcttaaactc ttttgaagct
     8881 atgctgctaa ccaggtatag gccagaaagg aggtggccca gtcagtacct tgctcacgtc
     8941 agctaagggg gacagtgatg tcatgtgggt gactcttctt catttgacca aggccagcct
     9001 cctgttctta cctctgccca ttcatttctg gctatgggaa aactaccacc tgttgagtga
     9061 gccaatgagg ccaggaagat cagaggggct gggttccggc ccatgggcca ctatttgaat
     9121 ttctctgtag taagctcttt gtagggctgg ctccaactgc agtagccaaa gaaaacctgt
     9181 ctgactcgac attttaatgc ctgagagttg gtgaaagttc agctgaagct aatgtctaga
     9241 catcagcagt aactaggcat ccagaaagta gcttcaacca aagctaatag agcccaagtt
     9301 tccgcaggaa gtttggtgct gagctgtcca gagcagttgt ttatctgagt gttgctagcc
     9361 cttagccatg ctttcttagc tttattctcg cctgttccta ggcatagcct gaaccagagc
     9421 tatggctcac tgctcgcttt attctactcc ctagtcatgc ggaacaacat agtctaaaaa
     9481 ggaagatgtg gactggggag ttaggagacc tgatttctat tccctatgct tctactaact
     9541 agcctcgaga tcttaaccgc tctgtgcctc agtgttccca tctgcaaaat ggggatagtg
     9601 ttaattgccc tacctacctc acagggttgt tgtgaggata aactgagaca atgaattcaa
     9661 agcaagttga caagtaagtg ctatgcaaat tgtaagaaat ggaaacaatc atgttaaaag
     9721 caatggaatg gacattggca gaagggttaa tggattgtcc agccctttct ctgtgtggct
     9781 taaacccagg tcgccattgc tttatacatt ttcacttagc acagatttgt aattatgcat
     9841 gtaataaagc acagccttat cca
//
