LOCUS       NM_001830               6759 bp    mRNA    linear   PRI 09-OCT-2024
DEFINITION  Homo sapiens chloride voltage-gated channel 4 (CLCN4), transcript
            variant 1, mRNA.
ACCESSION   NM_001830 XM_005274443
VERSION     NM_001830.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6759)
  AUTHORS   Sahly,A.N., Sierra-Marquez,J., Bungert-Plumke,S., Franzen,A.,
            Mougharbel,L., Berrahmoune,S., Dassi,C., Poulin,C., Srour,M.,
            Guzman,R.E. and Myers,K.A.
  TITLE     Genotype-phenotype correlation in CLCN4-related developmental and
            epileptic encephalopathy
  JOURNAL   Hum Genet 143 (5), 667-681 (2024)
   PUBMED   38578438
  REMARK    GeneRIF: Genotype-phenotype correlation in CLCN4-related
            developmental and epileptic encephalopathy.
REFERENCE   2  (bases 1 to 6759)
  AUTHORS   Palmer,E.E., Pusch,M., Picollo,A., Forwood,C., Nguyen,M.H.,
            Suckow,V., Gibbons,J., Hoff,A., Sigfrid,L., Megarbane,A., Nizon,M.,
            Cogne,B., Beneteau,C., Alkuraya,F.S., Chedrawi,A., Hashem,M.O.,
            Stamberger,H., Weckhuysen,S., Vanlander,A., Ceulemans,B.,
            Rajagopalan,S., Nunn,K., Arpin,S., Raynaud,M., Motter,C.S.,
            Ward-Melver,C., Janssens,K., Meuwissen,M., Beysen,D., Dikow,N.,
            Grimmel,M., Haack,T.B., Clement,E., McTague,A., Hunt,D.,
            Townshend,S., Ward,M., Richards,L.J., Simons,C., Costain,G.,
            Dupuis,L., Mendoza-Londono,R., Dudding-Byth,T., Boyle,J.,
            Saunders,C., Fleming,E., El Chehadeh,S., Spitz,M.A., Piton,A.,
            Gerard,B., Abi Warde,M.T., Rea,G., McKenna,C., Douzgou,S.,
            Banka,S., Akman,C., Bain,J.M., Sands,T.T., Wilson,G.N.,
            Silvertooth,E.J., Miller,L., Lederer,D., Sachdev,R., Macintosh,R.,
            Monestier,O., Karadurmus,D., Collins,F., Carter,M., Rohena,L.,
            Willemsen,M.H., Ockeloen,C.W., Pfundt,R., Kroft,S.D., Field,M.,
            Laranjeira,F.E.R., Fortuna,A.M., Soares,A.R., Michaud,V.,
            Naudion,S., Golla,S., Weaver,D.D., Bird,L.M., Friedman,J.,
            Clowes,V., Joss,S., Polsler,L., Campeau,P.M., Blazo,M.,
            Bijlsma,E.K., Rosenfeld,J.A., Beetz,C., Powis,Z., McWalter,K.,
            Brandt,T., Torti,E., Mathot,M., Mohammad,S.S., Armstrong,R. and
            Kalscheuer,V.M.
  TITLE     Functional and clinical studies reveal pathophysiological
            complexity of CLCN4-related neurodevelopmental condition
  JOURNAL   Mol Psychiatry 28 (2), 668-697 (2023)
   PUBMED   36385166
  REMARK    GeneRIF: Functional and clinical studies reveal pathophysiological
            complexity of CLCN4-related neurodevelopmental condition.
REFERENCE   3  (bases 1 to 6759)
  AUTHORS   He,H., Guzman,R.E., Cao,D., Sierra-Marquez,J., Yin,F., Fahlke,C.,
            Peng,J. and Stauber,T.
  TITLE     The molecular and phenotypic spectrum of CLCN4-related epilepsy
  JOURNAL   Epilepsia 62 (6), 1401-1415 (2021)
   PUBMED   33951195
  REMARK    GeneRIF: The molecular and phenotypic spectrum of CLCN4-related
            epilepsy.
REFERENCE   4  (bases 1 to 6759)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   5  (bases 1 to 6759)
  AUTHORS   Lamb,F.S., Clayton,G.H., Liu,B.X., Smith,R.L., Barna,T.J. and
            Schutte,B.C.
  TITLE     Expression of CLCN voltage-gated chloride channel genes in human
            blood vessels
  JOURNAL   J Mol Cell Cardiol 31 (3), 657-666 (1999)
   PUBMED   10198195
REFERENCE   6  (bases 1 to 6759)
  AUTHORS   Claes,S., Vogels,A., Holvoet,M., Devriendt,K., Raeymaekers,P.,
            Cassiman,J.J. and Fryns,J.P.
  TITLE     Regional localization of two genes for nonspecific X-linked mental
            retardation to Xp22.3-p22.2 (MRX49) and Xp11.3-p11.21 (MRX50)
  JOURNAL   Am J Med Genet 73 (4), 474-479 (1997)
   PUBMED   9415477
REFERENCE   7  (bases 1 to 6759)
  AUTHORS   Dinulos,M.B., Bassi,M.T., Rugarli,E.I., Chapman,V., Ballabio,A. and
            Disteche,C.M.
  TITLE     A new region of conservation is defined between human and mouse X
            chromosomes
  JOURNAL   Genomics 35 (1), 244-247 (1996)
   PUBMED   8661129
REFERENCE   8  (bases 1 to 6759)
  AUTHORS   Schnur,R.E. and Wick,P.A.
  TITLE     Intragenic TaqI restriction fragment length polymorphism (RFLP) in
            CICN4, between the loci for X-linked ocular albinism (OA1) and
            microphthalmia with linear skin defects syndrome (MLS)
  JOURNAL   Hum Genet 95 (5), 594-595 (1995)
   PUBMED   7759088
REFERENCE   9  (bases 1 to 6759)
  AUTHORS   van Slegtenhorst,M.A., Bassi,M.T., Borsani,G., Wapenaar,M.C.,
            Ferrero,G.B., de Conciliis,L., Rugarli,E.I., Grillo,A., Franco,B.,
            Zoghbi,H.Y. and Ballabio,A.
  TITLE     A gene from the Xp22.3 region shares homology with voltage-gated
            chloride channels
  JOURNAL   Hum Mol Genet 3 (4), 547-552 (1994)
   PUBMED   8069296
REFERENCE   10 (bases 1 to 6759)
  AUTHORS   Palmer,E.E., Nguyen,M.H., Forwood,C. and Kalscheuer,V.
  TITLE     CLCN4-Related Neurodevelopmental Disorder
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   34928551
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BP310547.1, AB019432.1 and
            AC003666.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_001830.3.
            
            Summary: The CLCN family of voltage-dependent chloride channel
            genes comprises nine members (CLCN1-7, Ka and Kb) which demonstrate
            quite diverse functional characteristics while sharing significant
            sequence homology. Chloride channel 4 has an evolutionary conserved
            CpG island and is conserved in both mouse and hamster. This gene is
            mapped in close proximity to APXL (Apical protein Xenopus
            laevis-like) and OA1 (Ocular albinism type I), which are both
            located on the human X chromosome at band p22.3. The physiological
            role of chloride channel 4 remains unknown but may contribute to
            the pathogenesis of neuronal disorders. Alternate splicing results
            in two transcript variants that encode different proteins.
            [provided by RefSeq, Mar 2012].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803611.127665.1,
                                           SRR1803617.93164.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1968968
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000380833.9/ ENSP00000370213.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-126               BP310547.1         31-156
            127-4579            AB019432.1         2-4454
            4580-6759           AC003666.1         105114-107293       c
FEATURES             Location/Qualifiers
     source          1..6759
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="X"
                     /map="Xp22.2"
     gene            1..6759
                     /gene="CLCN4"
                     /gene_synonym="ClC-4; ClC-4A; CLC4; MRX15; MRX49; MRXSRC"
                     /note="chloride voltage-gated channel 4"
                     /db_xref="GeneID:1183"
                     /db_xref="HGNC:HGNC:2022"
                     /db_xref="MIM:302910"
     exon            1..126
                     /gene="CLCN4"
                     /gene_synonym="ClC-4; ClC-4A; CLC4; MRX15; MRX49; MRXSRC"
                     /inference="alignment:Splign:2.1.0"
     exon            127..389
                     /gene="CLCN4"
                     /gene_synonym="ClC-4; ClC-4A; CLC4; MRX15; MRX49; MRXSRC"
                     /inference="alignment:Splign:2.1.0"
     exon            390..544
                     /gene="CLCN4"
                     /gene_synonym="ClC-4; ClC-4A; CLC4; MRX15; MRX49; MRXSRC"
                     /inference="alignment:Splign:2.1.0"
     CDS             401..2683
                     /gene="CLCN4"
                     /gene_synonym="ClC-4; ClC-4A; CLC4; MRX15; MRX49; MRXSRC"
                     /note="isoform 1 is encoded by transcript variant 1;
                     h(+)/Cl(-) exchange transporter 4; chloride channel
                     protein 4; chloride transporter ClC-4; chloride channel 4;
                     chloride channel, voltage-sensitive 4"
                     /codon_start=1
                     /product="H(+)/Cl(-) exchange transporter 4 isoform 1"
                     /protein_id="NP_001821.2"
                     /db_xref="CCDS:CCDS14137.1"
                     /db_xref="GeneID:1183"
                     /db_xref="HGNC:HGNC:2022"
                     /db_xref="MIM:302910"
                     /translation="MVNAGAMSGSGNLMDFLDEPFPDVGTYEDFHTIDWLREKSRDTD
                     RHRKITSKSKESIWEFIKSLLDAWSGWVVMLLIGLLAGTLAGVIDLAVDWMTDLKEGV
                     CLSAFWYSHEQCCWTSNETTFEDRDKCPLWQKWSELLVNQSEGASAYILNYLMYILWA
                     LLFAFLAVSLVRVFAPYACGSGIPEIKTILSGFIIRGYLGKWTLLIKTVTLVLVVSSG
                     LSLGKEGPLVHVACCCGNFFSSLFSKYSKNEGKRREVLSAAAAAGVSVAFGAPIGGVL
                     FSLEEVSYYFPLKTLWRSFFAALVAAFTLRSINPFGNSRLVLFYVEYHTPWYMAELFP
                     FILLGVFGGLWGTLFIRCNIAWCRRRKTTRLGKYPVLEVIVVTAITAIIAYPNPYTRQ
                     STSELISELFNDCGALESSQLCDYINDPNMTRPVDDIPDRPAGVGVYTAMWQLALALI
                     FKIVVTIFTFGMKIPSGLFIPSMAVGAIAGRMVGIGVEQLAYHHHDWIIFRNWCRPGA
                     DCVTPGLYAMVGAAACLGGVTRMTVSLVVIMFELTGGLEYIVPLMAAAVTSKWVADAF
                     GKEGIYEAHIHLNGYPFLDVKDEFTHRTLATDVMRPRRGEPPLSVLTQDSMTVEDVET
                     LIKETDYNGFPVVVSRDSERLIGFAQRRELILAIKNARQRQEGIVSNSIMYFTEEPPE
                     LPANSPHPLKLRRILNLSPFTVTDHTPMETVVDIFRKLGLRQCLVTRSGRLLGIITKK
                     DVLRHMAQMANQDPESIMFN"
     exon            545..644
                     /gene="CLCN4"
                     /gene_synonym="ClC-4; ClC-4A; CLC4; MRX15; MRX49; MRXSRC"
                     /inference="alignment:Splign:2.1.0"
     exon            645..832
                     /gene="CLCN4"
                     /gene_synonym="ClC-4; ClC-4A; CLC4; MRX15; MRX49; MRXSRC"
                     /inference="alignment:Splign:2.1.0"
     exon            833..955
                     /gene="CLCN4"
                     /gene_synonym="ClC-4; ClC-4A; CLC4; MRX15; MRX49; MRXSRC"
                     /inference="alignment:Splign:2.1.0"
     exon            956..1162
                     /gene="CLCN4"
                     /gene_synonym="ClC-4; ClC-4A; CLC4; MRX15; MRX49; MRXSRC"
                     /inference="alignment:Splign:2.1.0"
     exon            1163..1243
                     /gene="CLCN4"
                     /gene_synonym="ClC-4; ClC-4A; CLC4; MRX15; MRX49; MRXSRC"
                     /inference="alignment:Splign:2.1.0"
     exon            1244..1789
                     /gene="CLCN4"
                     /gene_synonym="ClC-4; ClC-4A; CLC4; MRX15; MRX49; MRXSRC"
                     /inference="alignment:Splign:2.1.0"
     exon            1790..1976
                     /gene="CLCN4"
                     /gene_synonym="ClC-4; ClC-4A; CLC4; MRX15; MRX49; MRXSRC"
                     /inference="alignment:Splign:2.1.0"
     exon            1977..2375
                     /gene="CLCN4"
                     /gene_synonym="ClC-4; ClC-4A; CLC4; MRX15; MRX49; MRXSRC"
                     /inference="alignment:Splign:2.1.0"
     exon            2376..2592
                     /gene="CLCN4"
                     /gene_synonym="ClC-4; ClC-4A; CLC4; MRX15; MRX49; MRXSRC"
                     /inference="alignment:Splign:2.1.0"
     exon            2593..6759
                     /gene="CLCN4"
                     /gene_synonym="ClC-4; ClC-4A; CLC4; MRX15; MRX49; MRXSRC"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3859..3864
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CLCN4"
                     /gene_synonym="ClC-4; ClC-4A; CLC4; MRX15; MRX49; MRXSRC"
                     /note="hexamer: ATTAAA"
     polyA_site      3878
                     /gene="CLCN4"
                     /gene_synonym="ClC-4; ClC-4A; CLC4; MRX15; MRX49; MRXSRC"
                     /note="major polyA site"
     regulatory      6689..6694
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CLCN4"
                     /gene_synonym="ClC-4; ClC-4A; CLC4; MRX15; MRX49; MRXSRC"
                     /note="hexamer: ATTAAA"
     polyA_site      6709
                     /gene="CLCN4"
                     /gene_synonym="ClC-4; ClC-4A; CLC4; MRX15; MRX49; MRXSRC"
     regulatory      6728..6733
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CLCN4"
                     /gene_synonym="ClC-4; ClC-4A; CLC4; MRX15; MRX49; MRXSRC"
                     /note="hexamer: AACAAG"
     polyA_site      6759
                     /gene="CLCN4"
                     /gene_synonym="ClC-4; ClC-4A; CLC4; MRX15; MRX49; MRXSRC"
ORIGIN      
        1 gaaatcgaca tttgcctctg gcaggaaaat gagcttgtca gtttctgcct ccattagacc
       61 ctcaacccaa aggagcagga gatttcggta gcgttttaac tttatctcag aatctgaaag
      121 cggaaggcca ggcaagctgc acacatcaag cgaaacgcct gaggggcggc cagcgcgagg
      181 gtttctggcc atcgaccctc acctcccggg acttccaggg tcttcccccc accccgcgca
      241 cacctccctg cctcgccccg agggcgtcac gtggcagcgt ggggcccgcc tcctggtgat
      301 gtcacggcgc tcgcagccgt cgcgctgaag aaaggatgct cgaggatgct gtccaggtgg
      361 gcggccgcgg gcgcgatgcg gcactgcagg tgtaattagc atggtcaatg cgggagcgat
      421 gagtggctct ggaaacctga tggatttcct cgatgagccg ttccctgatg tggggacgta
      481 tgaggacttc cacaccatcg actggctaag ggaaaagtca cgggacaccg acagacacag
      541 gaagatcacc agcaagagca aggagtccat atgggagttc atcaagagcc tgctggatgc
      601 ctggtcggga tgggtggtga tgctgctcat cggcctgctg gcgggcacct tggctggggt
      661 catcgatctc gccgtggact ggatgacgga cctgaaggag ggggtctgcc tgtctgcctt
      721 ctggtatagc catgagcagt gttgctggac ttctaacgag accacttttg aggacagaga
      781 caagtgtccc ctgtggcaga aatggtcgga gctgctggtg aatcagtcag agggtgccag
      841 tgcttacatt ctgaattact taatgtacat cctatgggcg ctgctgtttg catttttggc
      901 tgtctccctg gtgcgtgtat ttgcaccata tgcctgtggc tctggcatac cagagataaa
      961 gaccattttg agcggcttta tcatcagggg ctacttgggg aagtggaccc tgctaatcaa
     1021 gacagtcacg ctggtgctgg tagtgtcctc cggtctgagc cttgggaagg aagggccgct
     1081 agtgcacgtg gcttgttgct gtggcaactt cttcagcagc cttttctcca agtacagcaa
     1141 gaatgagggc aagaggcggg aggtgctttc agctgcagcg gctgctggag tctctgttgc
     1201 ctttggtgca ccaattggag gcgtgctttt cagtctagaa gaggtcagtt actactttcc
     1261 cctgaagacc ttgtggaggt catttttcgc agccctggtg gcggccttta cgctgagatc
     1321 catcaatccc tttgggaata gccgtctcgt tctcttttat gtggaatacc acacgccctg
     1381 gtacatggct gaactcttcc ccttcatcct gcttggggtc ttcgggggct tgtggggaac
     1441 cctcttcatc cgctgcaaca tcgcctggtg caggaggcgc aagaccacca ggctggggaa
     1501 gtacccggtg ctggaggtca ttgtggtgac tgccatcact gccatcattg cctaccccaa
     1561 tccctacaca cgccagagca ccagcgagct catttctgag ctgttcaatg actgtggagc
     1621 ccttgagtct tcccagctct gtgactacat caatgacccc aacatgactc ggcctgtgga
     1681 tgacattcca gaccggccgg ctggtgtcgg tgtttacacg gccatgtggc agctggccct
     1741 ggcactgatc ttcaaaatcg tcgttaccat atttaccttt ggcatgaaga tcccgtcggg
     1801 cctcttcatc cccagcatgg ctgtgggcgc gatagcgggc aggatggtgg gaattggcgt
     1861 ggagcagctg gcctaccatc accatgactg gatcatcttc aggaactggt gcagacccgg
     1921 tgcagactgt gtcacgccag ggctgtacgc aatggtggga gctgcggcct gcctcggtgg
     1981 agttaccagg atgacggtgt cattggtggt catcatgttt gaattaaccg ggggtctgga
     2041 gtacatcgtg cccctgatgg cggcggctgt gaccagcaag tgggtagctg atgcatttgg
     2101 gaaagaaggc atctacgagg cccacatcca cttaaatggg taccctttcc ttgacgtgaa
     2161 ggacgagttt actcaccgca cactggccac cgacgtcatg cggccccggc ggggagagcc
     2221 gccactgtcg gtgctcaccc aggacagcat gactgtcgag gacgtggaga cgctcatcaa
     2281 ggagaccgac tacaacggct tccccgtggt ggtctccaga gactccgagc gcctcattgg
     2341 atttgcccag aggagggaac tgattctcgc aataaagaac gccagacaga ggcaggaggg
     2401 cattgtgagc aattccatca tgtacttcac ggaggaaccc cccgagctgc cggccaacag
     2461 cccacatccc ctgaagctgc ggcgcatcct gaacctcagc ccgtttacag tgacagacca
     2521 cactccgatg gaaacggtgg tggatatctt ccggaaactg gggcttcggc agtgcctggt
     2581 gacgcggagc gggagacttc ttggcatcat cacaaaaaag gatgttctga gacatatggc
     2641 ccagatggca aaccaggacc ccgaatccat catgtttaat tagcaacaag gtggcaatta
     2701 ttttcagaaa aacactgact gtgtcattta aaaagaaata aatgatatgt tattatccca
     2761 atgaaagatc atgcattggg gacagcagaa acaaaagctt ttttggaaag gcggggaaga
     2821 aggatgaaac ctttaaaaac aaaaacaaaa acatcaatga gtaggcattt tatagcttta
     2881 accccgtatg agtttcaagc tgtgtttcct aatgagtttg ctactgctgt gggggcatgt
     2941 gggtgggtaa atgatgtaaa tgatgtgatc tgtacaagta tgtggagcat gaatgctgac
     3001 tcaagaaact tttactcctt ctgctcaagg ctgatgtttg taacttatga acacacgtga
     3061 agtgttgagt ccaaaagaca aaggggcatc ggcatgtcag cgtccttatt tattggttct
     3121 tgaagttttg ctgctatgtt actgaatcat actaaagaca tttgcgctta ctttgttgaa
     3181 aaagaaaaag aaattaaatt tgaacacagt gaaagctgca agtatgccag tgggtccgct
     3241 cacgaccgtc ctgtttcttg ctcctgttct cagcctgtta tctggcttat ccatcagttt
     3301 ctgaaccaac atcaggggca cctgccatga tgagtgtgaa gaaatcattt ctctatgcaa
     3361 gactggcatc ctgggactgc acgatgaact attggagcat acagtactgc ggtgtgtctc
     3421 ctcccataca aagggacaca tccttatttt gagggtatac tcacccgatc attgatttgc
     3481 atccccatgg catttgcttc atttatttat tgcttttgcc catgcattca atattgatca
     3541 gctcagcact tccatgggcc atagccggta tgcccccagc ctgccataag cgtgctttca
     3601 ttttcaagta gcatatgcaa gagtccaaac aggctaccca atgttactac ttatttttcc
     3661 tctcaggtct tatcagatac ggctgtggag aggatgaggc atatgcgttg tcagttggaa
     3721 tccctccaaa tggattgcaa gagaaatact aaattgattc taaatctgtt ccattatctt
     3781 ctctgtagga tgcaaaacat ccagttgtaa tgtataagca tgcacttcat actagcaatc
     3841 acagcacagg aggacagcat taaattttat atagccaatt tgggcactgg gcatctttgt
     3901 atttgaactt aacgacacat ttactctttt agtcaggatt tcggtctttt ctgcgggaag
     3961 gaattaagct atgtattagg tccaccatac gatctgtgta agtaagatta tcccacttgc
     4021 cctttctctt tagtctttgt cccttccttt tcactaagtg cccctcatcc ttttcaatct
     4081 cattgtcact ggatagtgct gtatcactta ggtgtgcatt cggctgcaaa taacagaaaa
     4141 cccagcaaac actgaagggc tggataagaa ccctgagagg gggtactcca ggactgtctc
     4201 cataacatca ttgggatccc catcccgttg ctatttcttt ttcaccgttt ttaccttttg
     4261 tcctctggct tattgcctca gagttgcaag atggttcctg aactgtcagg tatcatgtct
     4321 gctttctaag tgggaagagg aagggagagg caagcaacta ggtacaatgt ctatcaggaa
     4381 agcaaagctt ccccagagac gctcagcttg tatccattga ctggaagtct gtcacgtggc
     4441 tccccctagc cgcacactag tgtgggaagg aaaatattga gctaggcaca ttactctctg
     4501 aacgaaattc atattatctt attaaggaag agtgttggtc ttcaggaggg gaagtttgct
     4561 gtattggatg ccatcatcgt gtccttgtca ttgcccttcc ggttttcatt cttgctaaac
     4621 ccctgtgaat gttcttctaa ccttcctgtt ccccacccct tttctcagat ttgacctaga
     4681 attcccagcc caaatccata atttcttagc tctaatacga attttcatgt tggacaaaaa
     4741 cctagctaca aatgggtttc tatggaactt ctaattaatg tgcaaaatac atattttctc
     4801 caggttaaga aattttaagt cagatcatgc tgacacaata agaaaatttg tttgtgtaat
     4861 tcattgacct cttccttcca aaataacatc aagtagccac ctcagtgtga caatatccag
     4921 tcaatagtag agaatttaat ccttggtcct ataaaagaat aaaattcatt gtcgtattta
     4981 tagcacgtaa gccttgagta acaggtctta atgttagtgt tggtgttgtg gtttctgcgg
     5041 aacgtttaca agtgaagttg gattctcctg ggctttaaat ccaaaaagtc ttaatgaccg
     5101 cttcatgtag ttcccctctt attcccctgt ggtctcctca gagccagcgt gtgctgggaa
     5161 agagtattag tcatagcctg gctttataga atccaggagg tccttttagc tgaaacaagt
     5221 ggcttcaaaa gggaacatga aacagtgaaa agatagccaa aggtcagtga aaccacagca
     5281 tttctttcta atctggctga ggccatgggg gggaatgagg ggaaattatg cttccgggcc
     5341 aaggtcaccg agggggacct tgggatttgt gtcctcccca gtgcttagag tagagatacc
     5401 tgcaaattca tggcaaacag ttggaagagt tacttgactg aaacaaccca atcttacttt
     5461 ctttcgtaag ttgaacctct gatttattgt ggggttcagg ctctcctcaa tagaaggcca
     5521 gagttggaca ggtggactaa ggtcaaggat gaggagtctg cagtggggac taccacagca
     5581 aactgccttc cttccgtgga gtcctaactc agccatgtgg tccactcacc caggggcccg
     5641 ggggctcaga caaaccccat ggggaacatg ctgccatgct ctgtctttct tgtaaacgta
     5701 gttgggtagg gatcccaagg gtatttgctc tctccaaatg cagaacttcc atccacttta
     5761 tctggtgatc tgatgaacag catggggacg tggaggtgaa ataagcatct ttcagaataa
     5821 ggtttttttt tttttcctct tccagaacca aaggctttag acatttgtag aactttctgg
     5881 attccttctg aatcgggttt taacataaaa aaccatcaac acatcatggg aaaggagctc
     5941 tttcatgata tacatcacat gtttcttgct ctcactgagg aaatgtgttg tacaaaagca
     6001 ccaaataagg gcaccctgga agtgacatgt gtgtttcttc taagtcgttt ttagtgcatc
     6061 ccctgcgatt gcatgatgct catgtggctt ttagggaaag gcagaatgtg ctagaagtcg
     6121 aagtcattca atattcagtg cttactgtta ctttgaacca ggtaatctca gttctttatt
     6181 cagtccttcc caaagacctt acaaaggaga tgtgtaaact gatagatttt gagcctaata
     6241 agtcacagaa atggaaaatt ttagagcagt ttgtttcact taggtgagaa ctgattactt
     6301 taaattacca acatgtgcaa attctactta gtattcatta ttagatgcta agctggaaaa
     6361 gatgagaaac ccaaggagaa ctattgaatt accacataat tgtaatatta tgctttaatt
     6421 ttgtatgggc agtgttctat cagactaagc ttctagcctt tgaaatgatt tacatgagga
     6481 gagagcctgt atatctgact tgggaaacat taagcagacc ctattttcac tggcgcggta
     6541 ataactattt atttgctatc ctagaggtgc tgggaatgag cctacgtgcg actttgatga
     6601 gatatattca catttggtgt tggaacgacc acatagcaac gtgttggttt ttttttattg
     6661 taacactctg tgtaaacgtt gacaccttat taaatattaa atgtctgaac tctattttta
     6721 aaaataaaac aagcacagag tctgcaacaa acatgtaaa
//
