LOCUS       NM_001829               6635 bp    mRNA    linear   PRI 02-APR-2024
DEFINITION  Homo sapiens chloride voltage-gated channel 3 (CLCN3), transcript
            variant b, mRNA.
ACCESSION   NM_001829
VERSION     NM_001829.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6635)
  AUTHORS   Zhang,H., Meng,L., Yin,L., Fan,T., Yu,L., Han,S., Wang,L.,
            Liang,W., Yang,X. and Sun,S.
  TITLE     ClC-3 silencing mediates lysosomal acidification arrest and
            autophagy inhibition to sensitize chemo-photothermal therapy
  JOURNAL   Int J Pharm 628, 122297 (2022)
   PUBMED   36261097
  REMARK    GeneRIF: ClC-3 silencing mediates lysosomal acidification arrest
            and autophagy inhibition to sensitize chemo-photothermal therapy.
REFERENCE   2  (bases 1 to 6635)
  AUTHORS   Duncan,A.R., Polovitskaya,M.M., Gaitan-Penas,H., Bertelli,S.,
            VanNoy,G.E., Grant,P.E., O'Donnell-Luria,A., Valivullah,Z.,
            Lovgren,A.K., England,E.M., Agolini,E., Madden,J.A.,
            Schmitz-Abe,K., Kritzer,A., Hawley,P., Novelli,A., Alfieri,P.,
            Colafati,G.S., Wieczorek,D., Platzer,K., Luppe,J., Koch-Hogrebe,M.,
            Abou Jamra,R., Neira-Fresneda,J., Lehman,A., Boerkoel,C.F.,
            Seath,K., Clarke,L., van Ierland,Y., Argilli,E., Sherr,E.H.,
            Maiorana,A., Diel,T., Hempel,M., Bierhals,T., Estevez,R.,
            Jentsch,T.J., Pusch,M. and Agrawal,P.B.
  CONSRTM   CAUSES Study
  TITLE     Unique variants in CLCN3, encoding an endosomal anion/proton
            exchanger, underlie a spectrum of neurodevelopmental disorders
  JOURNAL   Am J Hum Genet 108 (8), 1450-1465 (2021)
   PUBMED   34186028
  REMARK    GeneRIF: Unique variants in CLCN3, encoding an endosomal
            anion/proton exchanger, underlie a spectrum of neurodevelopmental
            disorders.
REFERENCE   3  (bases 1 to 6635)
  AUTHORS   Yamada,S., Suzuki,Y., Bernotiene,E., Giles,W.R., Imaizumi,Y. and
            Yamamura,H.
  TITLE     Swelling-activated ClC-3 activity regulates prostaglandin E2
            release in human OUMS-27 chondrocytes
  JOURNAL   Biochem Biophys Res Commun 537, 29-35 (2021)
   PUBMED   33383561
  REMARK    GeneRIF: Swelling-activated ClC-3 activity regulates prostaglandin
            E2 release in human OUMS-27 chondrocytes.
REFERENCE   4  (bases 1 to 6635)
  AUTHORS   Mu,H., Mu,L. and Gao,J.
  TITLE     Suppression of CLC-3 reduces the proliferation, invasion and
            migration of colorectal cancer through Wnt/beta-catenin signaling
            pathway
  JOURNAL   Biochem Biophys Res Commun 533 (4), 1240-1246 (2020)
   PUBMED   33069359
  REMARK    GeneRIF: Suppression of CLC-3 reduces the proliferation, invasion
            and migration of colorectal cancer through Wnt/beta-catenin
            signaling pathway.
REFERENCE   5  (bases 1 to 6635)
  AUTHORS   Gu,Z., Wang,L., Yao,X., Long,Q., Lee,K., Li,J., Yue,D., Yang,S.,
            Liu,Y., Li,N. and Li,Y.
  TITLE     ClC-3/SGK1 regulatory axis enhances the olaparib-induced antitumor
            effect in human stomach adenocarcinoma
  JOURNAL   Cell Death Dis 11 (10), 898 (2020)
   PUBMED   33093458
  REMARK    GeneRIF: ClC-3/SGK1 regulatory axis enhances the olaparib-induced
            antitumor effect in human stomach adenocarcinoma.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 6635)
  AUTHORS   Lamb,F.S., Clayton,G.H., Liu,B.X., Smith,R.L., Barna,T.J. and
            Schutte,B.C.
  TITLE     Expression of CLCN voltage-gated chloride channel genes in human
            blood vessels
  JOURNAL   J Mol Cell Cardiol 31 (3), 657-666 (1999)
   PUBMED   10198195
REFERENCE   7  (bases 1 to 6635)
  AUTHORS   Loo,M.A., Jensen,T.J., Cui,L., Hou,Y., Chang,X.B. and Riordan,J.R.
  TITLE     Perturbation of Hsp90 interaction with nascent CFTR prevents its
            maturation and accelerates its degradation by the proteasome
  JOURNAL   EMBO J 17 (23), 6879-6887 (1998)
   PUBMED   9843494
REFERENCE   8  (bases 1 to 6635)
  AUTHORS   Shepard,A.R. and Rae,J.L.
  TITLE     Ion transporters and receptors in cDNA libraries from lens and
            cornea epithelia
  JOURNAL   Curr Eye Res 17 (7), 708-719 (1998)
   PUBMED   9678416
REFERENCE   9  (bases 1 to 6635)
  AUTHORS   Taine,L., Coupry,I., Boisseau,P., Saura,R., Lacombe,D. and
            Arveiler,B.
  TITLE     Refined localisation of the voltage-gated chloride channel, CLCN3,
            to 4q33
  JOURNAL   Hum Genet 102 (2), 178-181 (1998)
   PUBMED   9521585
REFERENCE   10 (bases 1 to 6635)
  AUTHORS   Borsani,G., Rugarli,E.I., Taglialatela,M., Wong,C. and Ballabio,A.
  TITLE     Characterization of a human and murine gene (CLCN3) sharing
            similarities to voltage-gated chloride channels and to a yeast
            integral membrane protein
  JOURNAL   Genomics 27 (1), 131-141 (1995)
   PUBMED   7665160
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BX647119.1, BC136510.1,
            AC106878.3 and AA780395.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On May 17, 2019 this sequence version replaced NM_001829.3.
            
            Summary: This gene encodes a member of the voltage-gated chloride
            channel (ClC) family. The encoded protein is present in all cell
            types and localized in plasma membranes and in intracellular
            vesicles. It is a multi-pass membrane protein which contains a ClC
            domain and two additional C-terminal CBS (cystathionine
            beta-synthase) domains. The ClC domain catalyzes the selective flow
            of Cl- ions across cell membranes, and the CBS domain may have a
            regulatory function. This protein plays a role in both
            acidification and transmitter loading of GABAergic synaptic
            vesicles, and in smooth muscle cell activation and neointima
            formation. This protein is required for lysophosphatidic acid
            (LPA)-activated Cl- current activity and
            fibroblast-to-myofibroblast differentiation. The protein activity
            is regulated by Ca(2+)/calmodulin-dependent protein kinase II
            (CaMKII) in glioma cells. Multiple alternatively spliced transcript
            variants encoding different isoforms have been identified.
            [provided by RefSeq, Aug 2011].
            
            Transcript Variant: This variant (b) represents the predominant
            transcript and encodes isoform b.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660805.183077.1,
                                           SRR1803616.275140.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000513761.6/ ENSP00000424603.1
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1127              BX647119.1         8-1134
            1128-3325           BC136510.1         960-3157
            3326-5677           AC106878.3         62558-64909
            5678-6149           AA780395.1         18-489              c
            6150-6635           AC106878.3         65382-65867
FEATURES             Location/Qualifiers
     source          1..6635
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="4"
                     /map="4q33"
     gene            1..6635
                     /gene="CLCN3"
                     /gene_synonym="ClC-3; CLC3; NEDHYBA; NEDSBA"
                     /note="chloride voltage-gated channel 3"
                     /db_xref="GeneID:1182"
                     /db_xref="HGNC:HGNC:2021"
                     /db_xref="MIM:600580"
     exon            1..486
                     /gene="CLCN3"
                     /gene_synonym="ClC-3; CLC3; NEDHYBA; NEDSBA"
                     /inference="alignment:Splign:2.1.0"
     exon            487..662
                     /gene="CLCN3"
                     /gene_synonym="ClC-3; CLC3; NEDHYBA; NEDSBA"
                     /inference="alignment:Splign:2.1.0"
     CDS             503..2959
                     /gene="CLCN3"
                     /gene_synonym="ClC-3; CLC3; NEDHYBA; NEDSBA"
                     /note="isoform b is encoded by transcript variant b;
                     h(+)/Cl(-) exchange transporter 3; chloride channel
                     protein 3; chloride transporter ClC-3; chloride channel 3;
                     chloride channel, voltage-sensitive 3"
                     /codon_start=1
                     /product="H(+)/Cl(-) exchange transporter 3 isoform b"
                     /protein_id="NP_001820.2"
                     /db_xref="CCDS:CCDS34101.1"
                     /db_xref="GeneID:1182"
                     /db_xref="HGNC:HGNC:2021"
                     /db_xref="MIM:600580"
                     /translation="MESEQLFHRGYYRNSYNSITSASSDEELLDGAGVIMDFQTSEDD
                     NLLDGDTAVGTHYTMTNGGSINSSTHLLDLLDEPIPGVGTYDDFHTIDWVREKCKDRE
                     RHRRINSKKKESAWEMTKSLYDAWSGWLVVTLTGLASGALAGLIDIAADWMTDLKEGI
                     CLSALWYNHEQCCWGSNETTFEERDKCPQWKTWAELIIGQAEGPGSYIMNYIMYIFWA
                     LSFAFLAVSLVKVFAPYACGSGIPEIKTILSGFIIRGYLGKWTLMIKTITLVLAVASG
                     LSLGKEGPLVHVACCCGNIFSYLFPKYSTNEAKKREVLSAASAAGVSVAFGAPIGGVL
                     FSLEEVSYYFPLKTLWRSFFAALVAAFVLRSINPFGNSRLVLFYVEYHTPWYLFELFP
                     FILLGVFGGLWGAFFIRANIAWCRRRKSTKFGKYPVLEVIIVAAITAVIAFPNPYTRL
                     NTSELIKELFTDCGPLESSSLCDYRNDMNASKIVDDIPDRPAGIGVYSAIWQLCLALI
                     FKIIMTVFTFGIKVPSGLFIPSMAIGAIAGRIVGIAVEQLAYYHHDWFIFKEWCEVGA
                     DCITPGLYAMVGAAACLGGVTRMTVSLVVIVFELTGGLEYIVPLMAAVMTSKWVGDAF
                     GREGIYEAHIRLNGYPFLDAKEEFTHTTLAADVMRPRRNDPPLAVLTQDNMTVDDIEN
                     MINETSYNGFPVIMSKESQRLVGFALRRDLTIAIESARKKQEGIVGSSRVCFAQHTPS
                     LPAESPRPLKLRSILDMSPFTVTDHTPMEIVVDIFRKLGLRQCLVTHNGRLLGIITKK
                     DILRHMAQTANQDPASIMFN"
     exon            663..820
                     /gene="CLCN3"
                     /gene_synonym="ClC-3; CLC3; NEDHYBA; NEDSBA"
                     /inference="alignment:Splign:2.1.0"
     exon            821..920
                     /gene="CLCN3"
                     /gene_synonym="ClC-3; CLC3; NEDHYBA; NEDSBA"
                     /inference="alignment:Splign:2.1.0"
     exon            921..1108
                     /gene="CLCN3"
                     /gene_synonym="ClC-3; CLC3; NEDHYBA; NEDSBA"
                     /inference="alignment:Splign:2.1.0"
     exon            1109..1231
                     /gene="CLCN3"
                     /gene_synonym="ClC-3; CLC3; NEDHYBA; NEDSBA"
                     /inference="alignment:Splign:2.1.0"
     exon            1232..1438
                     /gene="CLCN3"
                     /gene_synonym="ClC-3; CLC3; NEDHYBA; NEDSBA"
                     /inference="alignment:Splign:2.1.0"
     exon            1439..1519
                     /gene="CLCN3"
                     /gene_synonym="ClC-3; CLC3; NEDHYBA; NEDSBA"
                     /inference="alignment:Splign:2.1.0"
     exon            1520..2065
                     /gene="CLCN3"
                     /gene_synonym="ClC-3; CLC3; NEDHYBA; NEDSBA"
                     /inference="alignment:Splign:2.1.0"
     exon            2066..2252
                     /gene="CLCN3"
                     /gene_synonym="ClC-3; CLC3; NEDHYBA; NEDSBA"
                     /inference="alignment:Splign:2.1.0"
     exon            2253..2651
                     /gene="CLCN3"
                     /gene_synonym="ClC-3; CLC3; NEDHYBA; NEDSBA"
                     /inference="alignment:Splign:2.1.0"
     exon            2652..2868
                     /gene="CLCN3"
                     /gene_synonym="ClC-3; CLC3; NEDHYBA; NEDSBA"
                     /inference="alignment:Splign:2.1.0"
     exon            2869..6635
                     /gene="CLCN3"
                     /gene_synonym="ClC-3; CLC3; NEDHYBA; NEDSBA"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6069..6074
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CLCN3"
                     /gene_synonym="ClC-3; CLC3; NEDHYBA; NEDSBA"
                     /note="hexamer: AGTAAA"
     polyA_site      6097
                     /gene="CLCN3"
                     /gene_synonym="ClC-3; CLC3; NEDHYBA; NEDSBA"
                     /note="major polyA site"
     regulatory      6129..6134
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CLCN3"
                     /gene_synonym="ClC-3; CLC3; NEDHYBA; NEDSBA"
                     /note="hexamer: ATTAAA"
     polyA_site      6147
                     /gene="CLCN3"
                     /gene_synonym="ClC-3; CLC3; NEDHYBA; NEDSBA"
                     /note="major polyA site"
ORIGIN      
        1 ctcttcccct tccgtgggtc agggccggtc cggtccggaa cctgcagccc ctttcccagt
       61 gttctagttc gcccgtgacc cggaataatg agcaaggagg gtgtggtggg ttgaaagcca
      121 tcctacttta ctcccgagtt agagcatgga ttcagtttta gtcttaaggg ggaagtgaga
      181 ttggagattt ttatttttaa ttttgggcag aagcaggttg actctaggga tctccagagc
      241 gagaggattt aacttcatgt tgctcccgtg tttgaaggag gacaataaaa gtcccaccgg
      301 gcaaaatttt cgtaacctct gcggtagaaa acgtcaggta tcttttaaat cgcgatagtt
      361 ttcgctgtgt caggctttct tcggtggagc tccgagggta gctaggttct aggtttgaaa
      421 cagatgcaga atccaaaggc agcgcaaaaa acagccaccg attttgctat gtctctgagc
      481 tgcgagataa tcagacagct aaatggagtc tgagcagctg ttccatagag gctactatag
      541 aaacagctac aacagtataa caagtgcaag tagtgatgag gaacttttag atggagcagg
      601 tgttattatg gactttcaaa catctgaaga tgacaattta ttagatggtg acactgcagt
      661 tggaactcat tatacaatga caaatggagg cagcattaac agttctacac atttactgga
      721 tcttttggat gaaccaattc caggtgttgg tacatatgat gatttccata ctattgattg
      781 ggtgcgagaa aaatgtaaag acagagaaag gcatagacgg atcaacagca aaaagaaaga
      841 atcagcatgg gaaatgacaa aaagtttgta tgatgcgtgg tcaggatggc tagtagtaac
      901 actaacagga ttggcatcag gggcactggc cggattaata gacattgctg ccgattggat
      961 gactgaccta aaggagggca tttgccttag tgcgttgtgg tacaaccacg aacagtgctg
     1021 ttggggatct aatgaaacaa catttgaaga gagggataaa tgtccacagt ggaaaacatg
     1081 ggcagaatta atcataggtc aagcagaggg tcctggttct tatatcatga actacataat
     1141 gtacatcttc tgggccttga gttttgcctt tcttgcagtt tccctggtaa aggtatttgc
     1201 tccatatgcc tgtggctctg gaattccaga gattaaaact attttaagtg gattcatcat
     1261 cagaggttac ttgggaaaat ggactttaat gattaaaacc atcacattag tcctggctgt
     1321 ggcatcaggt ttgagtttag gaaaagaagg tcccctggta catgttgcct gttgctgcgg
     1381 aaatatcttt tcctacctct ttccaaagta tagcacaaac gaagctaaaa aaagggaggt
     1441 gctatcagct gcctcagctg caggggtttc tgtagctttt ggtgcaccaa ttggaggagt
     1501 tctttttagc ctggaagagg ttagctatta ttttcctctc aaaactttat ggagatcatt
     1561 ttttgctgct ttagtggctg catttgtttt gaggtccatc aatccatttg gtaacagccg
     1621 tctggtcctt ttttatgtgg agtatcatac accatggtac ctttttgaac tgtttccttt
     1681 tattcttcta ggggtatttg gagggctttg gggagccttt ttcattaggg caaatattgc
     1741 ctggtgtcgt cgacgcaagt ccacgaaatt tggaaagtat cccgttctgg aagtcattat
     1801 tgttgcagcc attactgctg tgatagcctt ccctaatcca tacactaggc taaacaccag
     1861 tgaactgatc aaagagcttt ttacagactg tggtcccctg gaatcctctt ctctttgtga
     1921 ctacagaaat gacatgaatg ccagtaaaat tgtcgatgac attcctgatc gtccagcagg
     1981 cattggagta tattcagcta tatggcagtt atgcctggca ctcatattta aaatcataat
     2041 gacagtattc acttttggca tcaaggttcc atcaggcttg ttcatcccca gcatggccat
     2101 tggagcgatc gcaggaagga ttgtggggat tgcggtggag cagcttgcct actatcacca
     2161 cgactggttt atctttaagg agtggtgtga ggtcggggct gattgcatta cacctggcct
     2221 ttatgccatg gttggtgctg ctgcatgctt aggtggtgtg acaagaatga ctgtctccct
     2281 ggtggttatt gtttttgagc ttactggagg cttggaatat attgttcccc ttatggctgc
     2341 agtcatgacc agtaaatggg ttggagatgc ctttggcagg gaaggcattt atgaagcaca
     2401 catccgatta aatggatacc ctttcttgga tgcaaaagaa gaattcactc ataccaccct
     2461 ggctgctgac gttatgagac ctcgaaggaa tgatcctccc ttagctgtcc tgacacagga
     2521 caatatgaca gtggatgata tagaaaacat gattaatgaa accagctaca atggatttcc
     2581 tgtcataatg tcaaaagaat ctcagagatt agtgggattt gccctcagaa gagacctgac
     2641 aattgcaata gaaagtgcca ggaaaaaaca agaaggtatc gttggcagtt ctcgggtgtg
     2701 ttttgcacag cacaccccat ctcttccagc agaaagtcct cggccattga agcttcgaag
     2761 cattcttgac atgagccctt ttacagtgac agaccacacc ccaatggaga tcgtggtgga
     2821 tattttccga aagctgggac tgaggcagtg ccttgtaact cacaatgggc gcctccttgg
     2881 cattataaca aaaaaagata tcctccggca tatggcccag acggcaaacc aagaccccgc
     2941 ttcaataatg ttcaactgaa tctcacagat gaggagagag aagaaacgga agaggaagtt
     3001 tatttgttga atagcacaac tctttaacct gagggagtca tctacttttt tttcctcctt
     3061 tacaaaaaaa gaaaggaaat ataaaagccg ggtttttgca acatggtttg caaataatgc
     3121 tggtggaatg gaggagttgt ttggggaggg aaaggagaga gaaggaaagg agtgaggtat
     3181 ttcccgtcta acagaaagca gcgtatcaac tcctattgtt ctgcactgga tgcattcagc
     3241 tgaggatgtg cctgatagtg caggcttgcg cctcaacaga gatgacagca gagtcctcga
     3301 gcacctggcc tgttgctcca acattgcaaa gacacattat cagtccctat ttctagaggg
     3361 attactttga attgagccat ctataaaact gcaaggtctt gccctttttt ttaatcaaaa
     3421 ctgttctgtt taattcatga attgtatagt taagcattac ctttctacat tccagaagag
     3481 cctttatttc tctctctctc tctctctctc tctctctctc tactgagctg taacaaagcc
     3541 tctttaaatc ggtgtatcct tttgaagcag tcctttctca tattgagatg tactgtgatt
     3601 ttactgaggt ttcatcacaa gaagggagtg tttcttgtgc cattaaccat gtagtttgta
     3661 ccatcactaa atgcttggaa cagtacacat gcaccacaac aaaggctcat caaacaggta
     3721 aagtctcgaa ggaagcgaga acgaaatctc tcattgtgtg ccgtgtggct caaaaccgaa
     3781 aacaatgaag cttggtttta aaggataaag ttttcttttt tgttttcctc tcagacttta
     3841 tggataatgt gaccgggtct tatgcaaatt ttctatttct aaaactacta ctatgatata
     3901 caagtgctgt tgagcataat taaataaaat gctgctgctt tgacagtaaa gagaaggaag
     3961 tattctgatt agctgtatct ggtattaatt gcatgttaaa acactggaat ttttaaaatt
     4021 gaaattagat cagtcattct tttcttttct caagatatct catggctgac actgaagaag
     4081 aaatgtaatt cataacttgc actaaatgta tatttttttt cttaaaaatt taccattctt
     4141 atttatattt ttatggatta aaatttataa aatacagatc agttaatatt gcacttaagt
     4201 aattttacct ttttaatgtg atttttatag aataattcag acttacaaat acagagatat
     4261 gaacaaagtt tacagtggga acaaaggttt aaaaaaaggt tgtggttctc tctctgtgat
     4321 ccagtgtgca cataaacctt tctctgatct ttcactgcca tcctctggat tatgtcttct
     4381 gacctgtcca ttttgaccca ttaactggaa agttgaaaaa ctacattaac tggaaagttg
     4441 aaaaactaca ttactttgga gaataaaacc gaaagttcgt gtataccttc ttaaaaaaaa
     4501 aatcaaacca aaaatgtgaa aacaatagaa ttgcaaagat agcagttaaa attttaatct
     4561 gaaaataacc tttgaatctc gggctaggtt acgtccatat ttgaagtggt cagtgatggt
     4621 ttgaacattt tttgcaggat gagtgaaaat gcactggatt atatttggga tttttgtttt
     4681 tggaattgtc tgttttaatc acagccttaa ttcacaattg gcaaaggcag tttactcaaa
     4741 ggactgggct aaatattctg taattatgca tttttgatag gaaaatgaaa tttttgcaaa
     4801 cagacatttt cttttttttt ggctggagtg cagtggggca tggtcttggc tcactgcagc
     4861 gttgaccacc tgggctcaag tgatactccc gcctcagcca cccaagtagc tggcactacg
     4921 ggcacacgcc accatgccca gctaattttt ttgtattttt agtagagatg gggttttgcc
     4981 atgctgccca ggctggtctc aactcctcag ctcaagcaat ctgcctgcgt gagcctccca
     5041 aagtggtgga attacaggcg tgggccactg cgcctggccc agacagacat tttctgaaac
     5101 acaactggca atgagctgtt tttacatttt gaaagtgatt cttcacttcc tagttcttaa
     5161 ttatagtata cctattaaga tctgtaagat cctgaagaca taagatcatg aagccatata
     5221 agaatgagga ttgaaagttg agcaaaattt tcgggatttt gggaaacatt cttagctgtg
     5281 ctatctgcct aaaattattc cttattactt ctctcctttg acagacttca agttttcttc
     5341 atagcccttt caaagttttt tgagccatcc agagtaaaat catttctaaa tgatagttct
     5401 gtatatctcc aactcgtctt aagtgtattt gcctgtgtgc aacgtattgc tagactatga
     5461 actcctcagc atggctgctg gataacttaa ttgtcctgag ttaatagcct tcaaaggaca
     5521 aatcggtttc tttgcagata gcttcgtaaa acttcacatg gagtttattt tatcatattt
     5581 ccctttttta tttctgctcc tcctttaatt gcccatcttg cttcagagac tgacatttca
     5641 gggtggatat taattaaagc attaattttg ttttttggta tatttctatc cctagtattt
     5701 ctatcttact gctaaaatac aggaaaagtg ccgtattttt aatgcattta gtggttttct
     5761 ttggtgttat ctgttccatt tttctttttc atacattgaa gtgtgtctcc ttttcaacca
     5821 aaataatgaa atagtggaga ccatgaaatt gttgtgcctg gctaattggc aaattaattt
     5881 accaatataa taagtgtagc gccttgtttg aatacccttt ttgagaaggt atgatgagaa
     5941 tgggcaaggg tgtcagcatc tcttcttctt aataattaat tgttttcagt tttggttcac
     6001 gaagaatgct tagttaatct gtaatgttgc ctagagctgt atttatctgt ttttatttat
     6061 actagtgtag taaagctgca tatcattaca gtaaaaacga ctactgtgat gagttaatca
     6121 gaaaatctat taaaatctat atgacaatgt gtggtttggc ctttaattat atctgtaaca
     6181 gtcctccctc ttcactgctg ccctacgcag tgtaggccga tatgcctggc acagggagcc
     6241 ccaaaaaggt aataattata atgcttctct caggttacaa agcatttcta cactttgcaa
     6301 gcaaggagca atagaagagt agatagatgc tggattgaca gcaggatgtt cataccacca
     6361 gcaatggttt agcgccccca tttgtcagtg aaagctgcag gtccacagaa aactgatttc
     6421 atttcagctc actttcaaaa gtgatttttt tttttttcgc aagaaaaatt acgctatttg
     6481 catgaaaaga ggtaaaacga tttatttaca tgtctttaaa aaatcacctt ggacatttgc
     6541 taatagaact gccaaaagag ggtggtaagt aacaaccttt gattaagatg tggtctttta
     6601 ctattaagct tttagtaaaa aggaacacac taaaa
//
