LOCUS       NM_001243372            6554 bp    mRNA    linear   PRI 02-APR-2024
DEFINITION  Homo sapiens chloride voltage-gated channel 3 (CLCN3), transcript
            variant a, mRNA.
ACCESSION   NM_001243372
VERSION     NM_001243372.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6554)
  AUTHORS   Zhang,H., Meng,L., Yin,L., Fan,T., Yu,L., Han,S., Wang,L.,
            Liang,W., Yang,X. and Sun,S.
  TITLE     ClC-3 silencing mediates lysosomal acidification arrest and
            autophagy inhibition to sensitize chemo-photothermal therapy
  JOURNAL   Int J Pharm 628, 122297 (2022)
   PUBMED   36261097
  REMARK    GeneRIF: ClC-3 silencing mediates lysosomal acidification arrest
            and autophagy inhibition to sensitize chemo-photothermal therapy.
REFERENCE   2  (bases 1 to 6554)
  AUTHORS   Duncan,A.R., Polovitskaya,M.M., Gaitan-Penas,H., Bertelli,S.,
            VanNoy,G.E., Grant,P.E., O'Donnell-Luria,A., Valivullah,Z.,
            Lovgren,A.K., England,E.M., Agolini,E., Madden,J.A.,
            Schmitz-Abe,K., Kritzer,A., Hawley,P., Novelli,A., Alfieri,P.,
            Colafati,G.S., Wieczorek,D., Platzer,K., Luppe,J., Koch-Hogrebe,M.,
            Abou Jamra,R., Neira-Fresneda,J., Lehman,A., Boerkoel,C.F.,
            Seath,K., Clarke,L., van Ierland,Y., Argilli,E., Sherr,E.H.,
            Maiorana,A., Diel,T., Hempel,M., Bierhals,T., Estevez,R.,
            Jentsch,T.J., Pusch,M. and Agrawal,P.B.
  CONSRTM   CAUSES Study
  TITLE     Unique variants in CLCN3, encoding an endosomal anion/proton
            exchanger, underlie a spectrum of neurodevelopmental disorders
  JOURNAL   Am J Hum Genet 108 (8), 1450-1465 (2021)
   PUBMED   34186028
  REMARK    GeneRIF: Unique variants in CLCN3, encoding an endosomal
            anion/proton exchanger, underlie a spectrum of neurodevelopmental
            disorders.
REFERENCE   3  (bases 1 to 6554)
  AUTHORS   Yamada,S., Suzuki,Y., Bernotiene,E., Giles,W.R., Imaizumi,Y. and
            Yamamura,H.
  TITLE     Swelling-activated ClC-3 activity regulates prostaglandin E2
            release in human OUMS-27 chondrocytes
  JOURNAL   Biochem Biophys Res Commun 537, 29-35 (2021)
   PUBMED   33383561
  REMARK    GeneRIF: Swelling-activated ClC-3 activity regulates prostaglandin
            E2 release in human OUMS-27 chondrocytes.
REFERENCE   4  (bases 1 to 6554)
  AUTHORS   Mu,H., Mu,L. and Gao,J.
  TITLE     Suppression of CLC-3 reduces the proliferation, invasion and
            migration of colorectal cancer through Wnt/beta-catenin signaling
            pathway
  JOURNAL   Biochem Biophys Res Commun 533 (4), 1240-1246 (2020)
   PUBMED   33069359
  REMARK    GeneRIF: Suppression of CLC-3 reduces the proliferation, invasion
            and migration of colorectal cancer through Wnt/beta-catenin
            signaling pathway.
REFERENCE   5  (bases 1 to 6554)
  AUTHORS   Gu,Z., Wang,L., Yao,X., Long,Q., Lee,K., Li,J., Yue,D., Yang,S.,
            Liu,Y., Li,N. and Li,Y.
  TITLE     ClC-3/SGK1 regulatory axis enhances the olaparib-induced antitumor
            effect in human stomach adenocarcinoma
  JOURNAL   Cell Death Dis 11 (10), 898 (2020)
   PUBMED   33093458
  REMARK    GeneRIF: ClC-3/SGK1 regulatory axis enhances the olaparib-induced
            antitumor effect in human stomach adenocarcinoma.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 6554)
  AUTHORS   Lamb,F.S., Clayton,G.H., Liu,B.X., Smith,R.L., Barna,T.J. and
            Schutte,B.C.
  TITLE     Expression of CLCN voltage-gated chloride channel genes in human
            blood vessels
  JOURNAL   J Mol Cell Cardiol 31 (3), 657-666 (1999)
   PUBMED   10198195
REFERENCE   7  (bases 1 to 6554)
  AUTHORS   Loo,M.A., Jensen,T.J., Cui,L., Hou,Y., Chang,X.B. and Riordan,J.R.
  TITLE     Perturbation of Hsp90 interaction with nascent CFTR prevents its
            maturation and accelerates its degradation by the proteasome
  JOURNAL   EMBO J 17 (23), 6879-6887 (1998)
   PUBMED   9843494
REFERENCE   8  (bases 1 to 6554)
  AUTHORS   Shepard,A.R. and Rae,J.L.
  TITLE     Ion transporters and receptors in cDNA libraries from lens and
            cornea epithelia
  JOURNAL   Curr Eye Res 17 (7), 708-719 (1998)
   PUBMED   9678416
REFERENCE   9  (bases 1 to 6554)
  AUTHORS   Taine,L., Coupry,I., Boisseau,P., Saura,R., Lacombe,D. and
            Arveiler,B.
  TITLE     Refined localisation of the voltage-gated chloride channel, CLCN3,
            to 4q33
  JOURNAL   Hum Genet 102 (2), 178-181 (1998)
   PUBMED   9521585
REFERENCE   10 (bases 1 to 6554)
  AUTHORS   Borsani,G., Rugarli,E.I., Taglialatela,M., Wong,C. and Ballabio,A.
  TITLE     Characterization of a human and murine gene (CLCN3) sharing
            similarities to voltage-gated chloride channels and to a yeast
            integral membrane protein
  JOURNAL   Genomics 27 (1), 131-141 (1995)
   PUBMED   7665160
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA102505.1, AK304362.1,
            AC106878.3 and AA780395.1.
            
            On May 31, 2019 this sequence version replaced NM_001243372.1.
            
            Summary: This gene encodes a member of the voltage-gated chloride
            channel (ClC) family. The encoded protein is present in all cell
            types and localized in plasma membranes and in intracellular
            vesicles. It is a multi-pass membrane protein which contains a ClC
            domain and two additional C-terminal CBS (cystathionine
            beta-synthase) domains. The ClC domain catalyzes the selective flow
            of Cl- ions across cell membranes, and the CBS domain may have a
            regulatory function. This protein plays a role in both
            acidification and transmitter loading of GABAergic synaptic
            vesicles, and in smooth muscle cell activation and neointima
            formation. This protein is required for lysophosphatidic acid
            (LPA)-activated Cl- current activity and
            fibroblast-to-myofibroblast differentiation. The protein activity
            is regulated by Ca(2+)/calmodulin-dependent protein kinase II
            (CaMKII) in glioma cells. Multiple alternatively spliced transcript
            variants encoding different isoforms have been identified.
            [provided by RefSeq, Aug 2011].
            
            Transcript Variant: This variant (a) lacks an in-frame exon in the
            middle coding region, compared to variant b. The resulting isoform
            (a) lacks an internal segment, compared to isoform b.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK304362.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA2142586
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-9                 DA102505.1         58-66
            10-3159             AK304362.1         1-3150
            3160-5596           AC106878.3         62473-64909
            5597-6068           AA780395.1         18-489              c
            6069-6554           AC106878.3         65382-65867
FEATURES             Location/Qualifiers
     source          1..6554
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="4"
                     /map="4q33"
     gene            1..6554
                     /gene="CLCN3"
                     /gene_synonym="ClC-3; CLC3; NEDHYBA; NEDSBA"
                     /note="chloride voltage-gated channel 3"
                     /db_xref="GeneID:1182"
                     /db_xref="HGNC:HGNC:2021"
                     /db_xref="MIM:600580"
     exon            1..486
                     /gene="CLCN3"
                     /gene_synonym="ClC-3; CLC3; NEDHYBA; NEDSBA"
                     /inference="alignment:Splign:2.1.0"
     exon            487..662
                     /gene="CLCN3"
                     /gene_synonym="ClC-3; CLC3; NEDHYBA; NEDSBA"
                     /inference="alignment:Splign:2.1.0"
     CDS             503..2878
                     /gene="CLCN3"
                     /gene_synonym="ClC-3; CLC3; NEDHYBA; NEDSBA"
                     /note="isoform a is encoded by transcript variant a;
                     h(+)/Cl(-) exchange transporter 3; chloride channel
                     protein 3; chloride transporter ClC-3; chloride channel 3;
                     chloride channel, voltage-sensitive 3"
                     /codon_start=1
                     /product="H(+)/Cl(-) exchange transporter 3 isoform a"
                     /protein_id="NP_001230301.1"
                     /db_xref="CCDS:CCDS58932.1"
                     /db_xref="GeneID:1182"
                     /db_xref="HGNC:HGNC:2021"
                     /db_xref="MIM:600580"
                     /translation="MESEQLFHRGYYRNSYNSITSASSDEELLDGAGVIMDFQTSEDD
                     NLLDGDTAVGTHYTMTNGGSINSSTHLLDLLDEPIPGVGTYDDFHTIDWVREKCKDRE
                     RHRRINSKKKESAWEMTKSLYDAWSGWLVVTLTGLASGALAGLIDIAADWMTDLKEGI
                     CLSALWYNHEQCCWGSNETTFEERDKCPQWKTWAELIIGQAEGPGSYIMNYIMYIFWA
                     LSFAFLAVSLVKVFAPYACGSGIPEIKTILSGFIIRGYLGKWTLMIKTITLVLAVASG
                     LSLGKEGPLVHVACCCGNIFSYLFPKYSTNEAKKREVSYYFPLKTLWRSFFAALVAAF
                     VLRSINPFGNSRLVLFYVEYHTPWYLFELFPFILLGVFGGLWGAFFIRANIAWCRRRK
                     STKFGKYPVLEVIIVAAITAVIAFPNPYTRLNTSELIKELFTDCGPLESSSLCDYRND
                     MNASKIVDDIPDRPAGIGVYSAIWQLCLALIFKIIMTVFTFGIKVPSGLFIPSMAIGA
                     IAGRIVGIAVEQLAYYHHDWFIFKEWCEVGADCITPGLYAMVGAAACLGGVTRMTVSL
                     VVIVFELTGGLEYIVPLMAAVMTSKWVGDAFGREGIYEAHIRLNGYPFLDAKEEFTHT
                     TLAADVMRPRRNDPPLAVLTQDNMTVDDIENMINETSYNGFPVIMSKESQRLVGFALR
                     RDLTIAIESARKKQEGIVGSSRVCFAQHTPSLPAESPRPLKLRSILDMSPFTVTDHTP
                     MEIVVDIFRKLGLRQCLVTHNGRLLGIITKKDILRHMAQTANQDPASIMFN"
     exon            663..820
                     /gene="CLCN3"
                     /gene_synonym="ClC-3; CLC3; NEDHYBA; NEDSBA"
                     /inference="alignment:Splign:2.1.0"
     exon            821..920
                     /gene="CLCN3"
                     /gene_synonym="ClC-3; CLC3; NEDHYBA; NEDSBA"
                     /inference="alignment:Splign:2.1.0"
     exon            921..1108
                     /gene="CLCN3"
                     /gene_synonym="ClC-3; CLC3; NEDHYBA; NEDSBA"
                     /inference="alignment:Splign:2.1.0"
     exon            1109..1231
                     /gene="CLCN3"
                     /gene_synonym="ClC-3; CLC3; NEDHYBA; NEDSBA"
                     /inference="alignment:Splign:2.1.0"
     exon            1232..1438
                     /gene="CLCN3"
                     /gene_synonym="ClC-3; CLC3; NEDHYBA; NEDSBA"
                     /inference="alignment:Splign:2.1.0"
     exon            1439..1984
                     /gene="CLCN3"
                     /gene_synonym="ClC-3; CLC3; NEDHYBA; NEDSBA"
                     /inference="alignment:Splign:2.1.0"
     exon            1985..2171
                     /gene="CLCN3"
                     /gene_synonym="ClC-3; CLC3; NEDHYBA; NEDSBA"
                     /inference="alignment:Splign:2.1.0"
     exon            2172..2570
                     /gene="CLCN3"
                     /gene_synonym="ClC-3; CLC3; NEDHYBA; NEDSBA"
                     /inference="alignment:Splign:2.1.0"
     exon            2571..2787
                     /gene="CLCN3"
                     /gene_synonym="ClC-3; CLC3; NEDHYBA; NEDSBA"
                     /inference="alignment:Splign:2.1.0"
     exon            2788..6554
                     /gene="CLCN3"
                     /gene_synonym="ClC-3; CLC3; NEDHYBA; NEDSBA"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5988..5993
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CLCN3"
                     /gene_synonym="ClC-3; CLC3; NEDHYBA; NEDSBA"
                     /note="hexamer: AGTAAA"
     polyA_site      6016
                     /gene="CLCN3"
                     /gene_synonym="ClC-3; CLC3; NEDHYBA; NEDSBA"
                     /note="major polyA site"
     regulatory      6048..6053
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CLCN3"
                     /gene_synonym="ClC-3; CLC3; NEDHYBA; NEDSBA"
                     /note="hexamer: ATTAAA"
     polyA_site      6066
                     /gene="CLCN3"
                     /gene_synonym="ClC-3; CLC3; NEDHYBA; NEDSBA"
                     /note="major polyA site"
ORIGIN      
        1 ctcttcccct tccgtgggtc agggccggtc cggtccggaa cctgcagccc ctttcccagt
       61 gttctagttc gcccgtgacc cggaataatg agcaaggagg gtgtggtggg ttgaaagcca
      121 tcctacttta ctcccgagtt agagcatgga ttcagtttta gtcttaaggg ggaagtgaga
      181 ttggagattt ttatttttaa ttttgggcag aagcaggttg actctaggga tctccagagc
      241 gagaggattt aacttcatgt tgctcccgtg tttgaaggag gacaataaaa gtcccaccgg
      301 gcaaaatttt cgtaacctct gcggtagaaa acgtcaggta tcttttaaat cgcgatagtt
      361 ttcgctgtgt caggctttct tcggtggagc tccgagggta gctaggttct aggtttgaaa
      421 cagatgcaga atccaaaggc agcgcaaaaa acagccaccg attttgctat gtctctgagc
      481 tgcgagataa tcagacagct aaatggagtc tgagcagctg ttccatagag gctactatag
      541 aaacagctac aacagtataa caagtgcaag tagtgatgag gaacttttag atggagcagg
      601 tgttattatg gactttcaaa catctgaaga tgacaattta ttagatggtg acactgcagt
      661 tggaactcat tatacaatga caaatggagg cagcattaac agttctacac atttactgga
      721 tcttttggat gaaccaattc caggtgttgg tacatatgat gatttccata ctattgattg
      781 ggtgcgagaa aaatgtaaag acagagaaag gcatagacgg atcaacagca aaaagaaaga
      841 atcagcatgg gaaatgacaa aaagtttgta tgatgcgtgg tcaggatggc tagtagtaac
      901 actaacagga ttggcatcag gggcactggc cggattaata gacattgctg ccgattggat
      961 gactgaccta aaggagggca tttgccttag tgcgttgtgg tacaaccacg aacagtgctg
     1021 ttggggatct aatgaaacaa catttgaaga gagggataaa tgtccacagt ggaaaacatg
     1081 ggcagaatta atcataggtc aagcagaggg tcctggttct tatatcatga actacataat
     1141 gtacatcttc tgggccttga gttttgcctt tcttgcagtt tccctggtaa aggtatttgc
     1201 tccatatgcc tgtggctctg gaattccaga gattaaaact attttaagtg gattcatcat
     1261 cagaggttac ttgggaaaat ggactttaat gattaaaacc atcacattag tcctggctgt
     1321 ggcatcaggt ttgagtttag gaaaagaagg tcccctggta catgttgcct gttgctgcgg
     1381 aaatatcttt tcctacctct ttccaaagta tagcacaaac gaagctaaaa aaagggaggt
     1441 tagctattat tttcctctca aaactttatg gagatcattt tttgctgctt tagtggctgc
     1501 atttgttttg aggtccatca atccatttgg taacagccgt ctggtccttt tttatgtgga
     1561 gtatcataca ccatggtacc tttttgaact gtttcctttt attcttctag gggtatttgg
     1621 agggctttgg ggagcctttt tcattagggc aaatattgcc tggtgtcgtc gacgcaagtc
     1681 cacgaaattt ggaaagtatc ccgttctgga agtcattatt gttgcagcca ttactgctgt
     1741 gatagccttc cctaatccat acactaggct aaacaccagt gaactgatca aagagctttt
     1801 tacagactgt ggtcccctgg aatcctcttc tctttgtgac tacagaaatg acatgaatgc
     1861 cagtaaaatt gtcgatgaca ttcctgatcg tccagcaggc attggagtat attcagctat
     1921 atggcagtta tgcctggcac tcatatttaa aatcataatg acagtattca cttttggcat
     1981 caaggttcca tcaggcttgt tcatccccag catggccatt ggagcgatcg caggaaggat
     2041 tgtggggatt gcggtggagc agcttgccta ctatcaccac gactggttta tctttaagga
     2101 gtggtgtgag gtcggggctg attgcattac acctggcctt tatgccatgg ttggtgctgc
     2161 tgcatgctta ggtggtgtga caagaatgac tgtctccctg gtggttattg tttttgagct
     2221 tactggaggc ttggaatata ttgttcccct tatggctgca gtcatgacca gtaaatgggt
     2281 tggagatgcc tttggcaggg aaggcattta tgaagcacac atccgattaa atggataccc
     2341 tttcttggat gcaaaagaag aattcactca taccaccctg gctgctgacg ttatgagacc
     2401 tcgaaggaat gatcctccct tagctgtcct gacacaggac aatatgacag tggatgatat
     2461 agaaaacatg attaatgaaa ccagctacaa tggatttcct gtcataatgt caaaagaatc
     2521 tcagagatta gtgggatttg ccctcagaag agacctgaca attgcaatag aaagtgccag
     2581 gaaaaaacaa gaaggtatcg ttggcagttc tcgggtgtgt tttgcacagc acaccccatc
     2641 tcttccagca gaaagtcctc ggccattgaa gcttcgaagc attcttgaca tgagcccttt
     2701 tacagtgaca gaccacaccc caatggagat cgtggtggat attttccgaa agctgggact
     2761 gaggcagtgc cttgtaactc acaatgggcg cctccttggc attataacaa aaaaagatat
     2821 cctccggcat atggcccaga cggcaaacca agaccccgct tcaataatgt tcaactgaat
     2881 ctcacagatg aggagagaga agaaacggaa gaggaagttt atttgttgaa tagcacaact
     2941 ctttaacctg agggagtcat ctactttttt ttcctccttt acaaaaaaag aaaggaaata
     3001 taaaagccgg gtttttgcaa catggtttgc aaataatgct ggtggaatgg aggagttgtt
     3061 tggggaggga aaggagagag aaggaaagga gtgaggtatt tcccgtctaa cagaaagcag
     3121 cgtatcaact cctattgttc tgcactggat gcattcagct gaggatgtgc ctgatagtgc
     3181 aggcttgcgc ctcaacagag atgacagcag agtcctcgag cacctggcct gttgctccaa
     3241 cattgcaaag acacattatc agtccctatt tctagaggga ttactttgaa ttgagccatc
     3301 tataaaactg caaggtcttg cccttttttt taatcaaaac tgttctgttt aattcatgaa
     3361 ttgtatagtt aagcattacc tttctacatt ccagaagagc ctttatttct ctctctctct
     3421 ctctctctct ctctctctct actgagctgt aacaaagcct ctttaaatcg gtgtatcctt
     3481 ttgaagcagt cctttctcat attgagatgt actgtgattt tactgaggtt tcatcacaag
     3541 aagggagtgt ttcttgtgcc attaaccatg tagtttgtac catcactaaa tgcttggaac
     3601 agtacacatg caccacaaca aaggctcatc aaacaggtaa agtctcgaag gaagcgagaa
     3661 cgaaatctct cattgtgtgc cgtgtggctc aaaaccgaaa acaatgaagc ttggttttaa
     3721 aggataaagt tttctttttt gttttcctct cagactttat ggataatgtg accgggtctt
     3781 atgcaaattt tctatttcta aaactactac tatgatatac aagtgctgtt gagcataatt
     3841 aaataaaatg ctgctgcttt gacagtaaag agaaggaagt attctgatta gctgtatctg
     3901 gtattaattg catgttaaaa cactggaatt tttaaaattg aaattagatc agtcattctt
     3961 ttcttttctc aagatatctc atggctgaca ctgaagaaga aatgtaattc ataacttgca
     4021 ctaaatgtat attttttttc ttaaaaattt accattctta tttatatttt tatggattaa
     4081 aatttataaa atacagatca gttaatattg cacttaagta attttacctt tttaatgtga
     4141 tttttataga ataattcaga cttacaaata cagagatatg aacaaagttt acagtgggaa
     4201 caaaggttta aaaaaaggtt gtggttctct ctctgtgatc cagtgtgcac ataaaccttt
     4261 ctctgatctt tcactgccat cctctggatt atgtcttctg acctgtccat tttgacccat
     4321 taactggaaa gttgaaaaac tacattaact ggaaagttga aaaactacat tactttggag
     4381 aataaaaccg aaagttcgtg tataccttct taaaaaaaaa atcaaaccaa aaatgtgaaa
     4441 acaatagaat tgcaaagata gcagttaaaa ttttaatctg aaaataacct ttgaatctcg
     4501 ggctaggtta cgtccatatt tgaagtggtc agtgatggtt tgaacatttt ttgcaggatg
     4561 agtgaaaatg cactggatta tatttgggat ttttgttttt ggaattgtct gttttaatca
     4621 cagccttaat tcacaattgg caaaggcagt ttactcaaag gactgggcta aatattctgt
     4681 aattatgcat ttttgatagg aaaatgaaat ttttgcaaac agacattttc tttttttttg
     4741 gctggagtgc agtggggcat ggtcttggct cactgcagcg ttgaccacct gggctcaagt
     4801 gatactcccg cctcagccac ccaagtagct ggcactacgg gcacacgcca ccatgcccag
     4861 ctaatttttt tgtattttta gtagagatgg ggttttgcca tgctgcccag gctggtctca
     4921 actcctcagc tcaagcaatc tgcctgcgtg agcctcccaa agtggtggaa ttacaggcgt
     4981 gggccactgc gcctggccca gacagacatt ttctgaaaca caactggcaa tgagctgttt
     5041 ttacattttg aaagtgattc ttcacttcct agttcttaat tatagtatac ctattaagat
     5101 ctgtaagatc ctgaagacat aagatcatga agccatataa gaatgaggat tgaaagttga
     5161 gcaaaatttt cgggattttg ggaaacattc ttagctgtgc tatctgccta aaattattcc
     5221 ttattacttc tctcctttga cagacttcaa gttttcttca tagccctttc aaagtttttt
     5281 gagccatcca gagtaaaatc atttctaaat gatagttctg tatatctcca actcgtctta
     5341 agtgtatttg cctgtgtgca acgtattgct agactatgaa ctcctcagca tggctgctgg
     5401 ataacttaat tgtcctgagt taatagcctt caaaggacaa atcggtttct ttgcagatag
     5461 cttcgtaaaa cttcacatgg agtttatttt atcatatttc ccttttttat ttctgctcct
     5521 cctttaattg cccatcttgc ttcagagact gacatttcag ggtggatatt aattaaagca
     5581 ttaattttgt tttttggtat atttctatcc ctagtatttc tatcttactg ctaaaataca
     5641 ggaaaagtgc cgtattttta atgcatttag tggttttctt tggtgttatc tgttccattt
     5701 ttctttttca tacattgaag tgtgtctcct tttcaaccaa aataatgaaa tagtggagac
     5761 catgaaattg ttgtgcctgg ctaattggca aattaattta ccaatataat aagtgtagcg
     5821 ccttgtttga ataccctttt tgagaaggta tgatgagaat gggcaagggt gtcagcatct
     5881 cttcttctta ataattaatt gttttcagtt ttggttcacg aagaatgctt agttaatctg
     5941 taatgttgcc tagagctgta tttatctgtt tttatttata ctagtgtagt aaagctgcat
     6001 atcattacag taaaaacgac tactgtgatg agttaatcag aaaatctatt aaaatctata
     6061 tgacaatgtg tggtttggcc tttaattata tctgtaacag tcctccctct tcactgctgc
     6121 cctacgcagt gtaggccgat atgcctggca cagggagccc caaaaaggta ataattataa
     6181 tgcttctctc aggttacaaa gcatttctac actttgcaag caaggagcaa tagaagagta
     6241 gatagatgct ggattgacag caggatgttc ataccaccag caatggttta gcgcccccat
     6301 ttgtcagtga aagctgcagg tccacagaaa actgatttca tttcagctca ctttcaaaag
     6361 tgattttttt ttttttcgca agaaaaatta cgctatttgc atgaaaagag gtaaaacgat
     6421 ttatttacat gtctttaaaa aatcaccttg gacatttgct aatagaactg ccaaaagagg
     6481 gtggtaagta acaacctttg attaagatgt ggtcttttac tattaagctt ttagtaaaaa
     6541 ggaacacact aaaa
//
