LOCUS       NM_006536               3935 bp    mRNA    linear   PRI 02-MAY-2024
DEFINITION  Homo sapiens chloride channel accessory 2 (CLCA2), mRNA.
ACCESSION   NM_006536
VERSION     NM_006536.7
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3935)
  AUTHORS   Seltmann,K., Hettich,B., Abele,S., Gurri,S., Mantella,V.,
            Leroux,J.C. and Werner,S.
  TITLE     Transport of CLCA2 to the nucleus by extracellular vesicles
            controls keratinocyte survival and migration
  JOURNAL   J Extracell Vesicles 13 (4), e12430 (2024)
   PUBMED   38602325
  REMARK    GeneRIF: Transport of CLCA2 to the nucleus by extracellular
            vesicles controls keratinocyte survival and migration.
REFERENCE   2  (bases 1 to 3935)
  AUTHORS   Fan,Y., Chen,S., Chu,C., Yin,X., Jin,J., Zhang,L., Yan,H., Cao,Z.,
            Liu,R., Xin,M., Li,L. and Yin,C.
  TITLE     TP63 truncating mutation causes increased cell apoptosis and
            premature ovarian insufficiency by enhanced transcriptional
            activation of CLCA2
  JOURNAL   J Ovarian Res 17 (1), 67 (2024)
   PUBMED   38528613
  REMARK    GeneRIF: TP63 truncating mutation causes increased cell apoptosis
            and premature ovarian insufficiency by enhanced transcriptional
            activation of CLCA2.
            Erratum:[J Ovarian Res. 2024 Apr 29;17(1):93. PMID: 38685112]
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3935)
  AUTHORS   Xin,W., Zhang,J., Zhang,H., Ma,X., Zhang,Y., Li,Y. and Wang,F.
  TITLE     CLCA2 overexpression suppresses epithelial-to-mesenchymal
            transition in cervical cancer cells through inactivation of
            ERK/JNK/p38-MAPK signaling pathways
  JOURNAL   BMC Mol Cell Biol 23 (1), 44 (2022)
   PUBMED   36280802
  REMARK    GeneRIF: CLCA2 overexpression suppresses epithelial-to-mesenchymal
            transition in cervical cancer cells through inactivation of
            ERK/JNK/p38-MAPK signaling pathways.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3935)
  AUTHORS   Yang,X., Cao,J.L., Yang,F.N., Li,X.F., Tao,L.M. and Wang,F.
  TITLE     Decreased expression of CLCA2 and the correlating with immune
            infiltrates in patients with cervical squamous cell carcinoma: A
            bioinformatics analysis
  JOURNAL   Taiwan J Obstet Gynecol 60 (3), 480-486 (2021)
   PUBMED   33966732
  REMARK    GeneRIF: Decreased expression of CLCA2 and the correlating with
            immune infiltrates in patients with cervical squamous cell
            carcinoma: A bioinformatics analysis.
REFERENCE   5  (bases 1 to 3935)
  AUTHORS   Purrington,K.S., Knight,J. 3rd, Dyson,G., Ali-Fehmi,R.,
            Schwartz,A.G., Boerner,J.L. and Bandyopadhyay,S.
  TITLE     CLCA2 expression is associated with survival among African American
            women with triple negative breast cancer
  JOURNAL   PLoS One 15 (4), e0231712 (2020)
   PUBMED   32298355
  REMARK    GeneRIF: CLCA2 was identified as a potential prognostic marker for
            triple negative breast cancer in African American women.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3935)
  AUTHORS   Yurtsever,Z., Sala-Rabanal,M., Randolph,D.T., Scheaffer,S.M.,
            Roswit,W.T., Alevy,Y.G., Patel,A.C., Heier,R.F., Romero,A.G.,
            Nichols,C.G., Holtzman,M.J. and Brett,T.J.
  TITLE     Self-cleavage of human CLCA1 protein by a novel internal
            metalloprotease domain controls calcium-activated chloride channel
            activation
  JOURNAL   J Biol Chem 287 (50), 42138-42149 (2012)
   PUBMED   23112050
REFERENCE   7  (bases 1 to 3935)
  AUTHORS   Gruber,A.D. and Pauli,B.U.
  TITLE     Tumorigenicity of human breast cancer is associated with loss of
            the Ca2+-activated chloride channel CLCA2
  JOURNAL   Cancer Res 59 (21), 5488-5491 (1999)
   PUBMED   10554024
REFERENCE   8  (bases 1 to 3935)
  AUTHORS   Gruber,A.D. and Pauli,B.U.
  TITLE     Clustering of the human CLCA gene family on the short arm of
            chromosome 1 (1p22-31)
  JOURNAL   Genome 42 (5), 1030-1032 (1999)
   PUBMED   10584316
REFERENCE   9  (bases 1 to 3935)
  AUTHORS   Agnel,M., Vermat,T. and Culouscou,J.M.
  TITLE     Identification of three novel members of the calcium-dependent
            chloride channel (CaCC) family predominantly expressed in the
            digestive tract and trachea
  JOURNAL   FEBS Lett 455 (3), 295-301 (1999)
   PUBMED   10437792
REFERENCE   10 (bases 1 to 3935)
  AUTHORS   Gruber,A.D., Schreur,K.D., Ji,H.L., Fuller,C.M. and Pauli,B.U.
  TITLE     Molecular cloning and transmembrane structure of hCLCA2 from human
            lung, trachea, and mammary gland
  JOURNAL   Am J Physiol 276 (6), C1261-C1270 (1999)
   PUBMED   10362588
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DC417810.1 and BC041096.1.
            
            On Nov 22, 2018 this sequence version replaced NM_006536.6.
            
            Summary: This gene encodes a member of the calcium-activated
            chloride channel regulator (CLCR) family of proteins. Members of
            this family regulate the transport of chloride across the plasma
            membrane. The encoded protein is autoproteolytically processed to
            generate N- and C- terminal fragments. Expression of this gene is
            upregulated by the tumor suppressor protein p53 in response to DNA
            damage. In breast cancer, expression of this gene is downregulated
            and the encoded protein may inhibit migration and invasion while
            promoting mesenchymal-to-epithelial transition in cancer cell
            lines. [provided by RefSeq, Sep 2016].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC041096.1, AB026833.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA2144335
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000370565.5/ ENSP00000359596.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-14                DC417810.1         289-302
            15-3935             BC041096.1         1-3921
FEATURES             Location/Qualifiers
     source          1..3935
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p22.3"
     gene            1..3935
                     /gene="CLCA2"
                     /gene_synonym="CACC; CaCC-3; CACC3; CLCRG2"
                     /note="chloride channel accessory 2"
                     /db_xref="GeneID:9635"
                     /db_xref="HGNC:HGNC:2016"
                     /db_xref="MIM:604003"
     exon            1..263
                     /gene="CLCA2"
                     /gene_synonym="CACC; CaCC-3; CACC3; CLCRG2"
                     /inference="alignment:Splign:2.1.0"
     CDS             78..2909
                     /gene="CLCA2"
                     /gene_synonym="CACC; CaCC-3; CACC3; CLCRG2"
                     /note="CLCA family member 2, chloride channel regulator;
                     chloride channel, calcium activated, family member 2;
                     calcium-activated chloride channel regulator 2;
                     calcium-activated chloride channel protein 3"
                     /codon_start=1
                     /product="calcium-activated chloride channel regulator 2
                     preproprotein"
                     /protein_id="NP_006527.1"
                     /db_xref="CCDS:CCDS708.1"
                     /db_xref="GeneID:9635"
                     /db_xref="HGNC:HGNC:2016"
                     /db_xref="MIM:604003"
                     /translation="MTQRSIAGPICNLKFVTLLVALSSELPFLGAGVQLQDNGYNGLL
                     IAINPQVPENQNLISNIKEMITEASFYLFNATKRRVFFRNIKILIPATWKANNNSKIK
                     QESYEKANVIVTDWYGAHGDDPYTLQYRGCGKEGKYIHFTPNFLLNDNLTAGYGSRGR
                     VFVHEWAHLRWGVFDEYNNDKPFYINGQNQIKVTRCSSDITGIFVCEKGPCPQENCII
                     SKLFKEGCTFIYNSTQNATASIMFMQSLSSVVEFCNASTHNQEAPNLQNQMCSLRSAW
                     DVITDSADFHHSFPMNGTELPPPPTFSLVQAGDKVVCLVLDVSSKMAEADRLLQLQQA
                     AEFYLMQIVEIHTFVGIASFDSKGEIRAQLHQINSNDDRKLLVSYLPTTVSAKTDISI
                     CSGLKKGFEVVEKLNGKAYGSVMILVTSGDDKLLGNCLPTVLSSGSTIHSIALGSSAA
                     PNLEELSRLTGGLKFFVPDISNSNSMIDAFSRISSGTGDIFQQHIQLESTGENVKPHH
                     QLKNTVTVDNTVGNDTMFLVTWQASGPPEIILFDPDGRKYYTNNFITNLTFRTASLWI
                     PGTAKPGHWTYTLNNTHHSLQALKVTVTSRASNSAVPPATVEAFVERDSLHFPHPVMI
                     YANVKQGFYPILNATVTATVEPETGDPVTLRLLDDGAGADVIKNDGIYSRYFFSFAAN
                     GRYSLKVHVNHSPSISTPAHSIPGSHAMYVPGYTANGNIQMNAPRKSVGRNEEERKWG
                     FSRVSSGGSFSVLGVPAGPHPDVFPPCKIIDLEAVKVEEELTLSWTAPGEDFDQGQAT
                     SYEIRMSKSLQNIQDDFNNAILVNTSKRNPQQAGIREIFTFSPQISTNGPEHQPNGET
                     HESHRIYVAIRAMDRNSLQSAVSNIAQAPLFIPPNSDPVPARDYLILKGVLTAMGLIG
                     IICLIIVVTHHTLSRKKRADKKENGTKLL"
     exon            264..401
                     /gene="CLCA2"
                     /gene_synonym="CACC; CaCC-3; CACC3; CLCRG2"
                     /inference="alignment:Splign:2.1.0"
     exon            402..552
                     /gene="CLCA2"
                     /gene_synonym="CACC; CaCC-3; CACC3; CLCRG2"
                     /inference="alignment:Splign:2.1.0"
     exon            553..661
                     /gene="CLCA2"
                     /gene_synonym="CACC; CaCC-3; CACC3; CLCRG2"
                     /inference="alignment:Splign:2.1.0"
     exon            662..821
                     /gene="CLCA2"
                     /gene_synonym="CACC; CaCC-3; CACC3; CLCRG2"
                     /inference="alignment:Splign:2.1.0"
     exon            822..1049
                     /gene="CLCA2"
                     /gene_synonym="CACC; CaCC-3; CACC3; CLCRG2"
                     /inference="alignment:Splign:2.1.0"
     exon            1050..1280
                     /gene="CLCA2"
                     /gene_synonym="CACC; CaCC-3; CACC3; CLCRG2"
                     /inference="alignment:Splign:2.1.0"
     exon            1281..1458
                     /gene="CLCA2"
                     /gene_synonym="CACC; CaCC-3; CACC3; CLCRG2"
                     /inference="alignment:Splign:2.1.0"
     exon            1459..1565
                     /gene="CLCA2"
                     /gene_synonym="CACC; CaCC-3; CACC3; CLCRG2"
                     /inference="alignment:Splign:2.1.0"
     exon            1566..1790
                     /gene="CLCA2"
                     /gene_synonym="CACC; CaCC-3; CACC3; CLCRG2"
                     /inference="alignment:Splign:2.1.0"
     exon            1791..2061
                     /gene="CLCA2"
                     /gene_synonym="CACC; CaCC-3; CACC3; CLCRG2"
                     /inference="alignment:Splign:2.1.0"
     exon            2062..2232
                     /gene="CLCA2"
                     /gene_synonym="CACC; CaCC-3; CACC3; CLCRG2"
                     /inference="alignment:Splign:2.1.0"
     exon            2233..2466
                     /gene="CLCA2"
                     /gene_synonym="CACC; CaCC-3; CACC3; CLCRG2"
                     /inference="alignment:Splign:2.1.0"
     exon            2467..3935
                     /gene="CLCA2"
                     /gene_synonym="CACC; CaCC-3; CACC3; CLCRG2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3848..3853
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CLCA2"
                     /gene_synonym="CACC; CaCC-3; CACC3; CLCRG2"
                     /note="hexamer: AATAAA"
     polyA_site      3872
                     /gene="CLCA2"
                     /gene_synonym="CACC; CaCC-3; CACC3; CLCRG2"
                     /note="major polyA site"
     regulatory      3913..3918
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CLCA2"
                     /gene_synonym="CACC; CaCC-3; CACC3; CLCRG2"
                     /note="hexamer: AATAAA"
     polyA_site      3935
                     /gene="CLCA2"
                     /gene_synonym="CACC; CaCC-3; CACC3; CLCRG2"
ORIGIN      
        1 atctgcatcc atattgaaaa cctgacacaa tgtatgcagc aggctcagtg tgagtgaact
       61 ggaggcttct ctacaacatg acccaaagga gcattgcagg tcctatttgc aacctgaagt
      121 ttgtgactct cctggttgcc ttaagttcag aactcccatt cctgggagct ggagtacagc
      181 ttcaagacaa tgggtataat ggattgctca ttgcaattaa tcctcaggta cctgagaatc
      241 agaacctcat ctcaaacatt aaggaaatga taactgaagc ttcattttac ctatttaatg
      301 ctaccaagag aagagtattt ttcagaaata taaagatttt aatacctgcc acatggaaag
      361 ctaataataa cagcaaaata aaacaagaat catatgaaaa ggcaaatgtc atagtgactg
      421 actggtatgg ggcacatgga gatgatccat acaccctaca atacagaggg tgtggaaaag
      481 agggaaaata cattcatttc acacctaatt tcctactgaa tgataactta acagctggct
      541 acggatcacg aggccgagtg tttgtccatg aatgggccca cctccgttgg ggtgtgttcg
      601 atgagtataa caatgacaaa cctttctaca taaatgggca aaatcaaatt aaagtgacaa
      661 ggtgttcatc tgacatcaca ggcatttttg tgtgtgaaaa aggtccttgc ccccaagaaa
      721 actgtattat tagtaagctt tttaaagaag gatgcacctt tatctacaat agcacccaaa
      781 atgcaactgc atcaataatg ttcatgcaaa gtttatcttc tgtggttgaa ttttgtaatg
      841 caagtaccca caaccaagaa gcaccaaacc tacagaacca gatgtgcagc ctcagaagtg
      901 catgggatgt aatcacagac tctgctgact ttcaccacag ctttcccatg aatgggactg
      961 agcttccacc tcctcccaca ttctcgcttg tacaggctgg tgacaaagtg gtctgtttag
     1021 tgctggatgt gtccagcaag atggcagagg ctgacagact ccttcaacta caacaagccg
     1081 cagaatttta tttgatgcag attgttgaaa ttcatacctt cgtgggcatt gccagtttcg
     1141 acagcaaagg agagatcaga gcccagctac accaaattaa cagcaatgat gatcgaaagt
     1201 tgctggtttc atatctgccc accactgtat cagctaaaac agacatcagc atttgttcag
     1261 ggcttaagaa aggatttgag gtggttgaaa aactgaatgg aaaagcttat ggctctgtga
     1321 tgatattagt gaccagcgga gatgataagc ttcttggcaa ttgcttaccc actgtgctca
     1381 gcagtggttc aacaattcac tccattgccc tgggttcatc tgcagcccca aatctggagg
     1441 aattatcacg tcttacagga ggtttaaagt tctttgttcc agatatatca aactccaata
     1501 gcatgattga tgctttcagt agaatttcct ctggaactgg agacattttc cagcaacata
     1561 ttcagcttga aagtacaggt gaaaatgtca aacctcacca tcaattgaaa aacacagtga
     1621 ctgtggataa tactgtgggc aacgacacta tgtttctagt tacgtggcag gccagtggtc
     1681 ctcctgagat tatattattt gatcctgatg gacgaaaata ctacacaaat aattttatca
     1741 ccaatctaac ttttcggaca gctagtcttt ggattccagg aacagctaag cctgggcact
     1801 ggacttacac cctgaacaat acccatcatt ctctgcaagc cctgaaagtg acagtgacct
     1861 ctcgcgcctc caactcagct gtgcccccag ccactgtgga agcctttgtg gaaagagaca
     1921 gcctccattt tcctcatcct gtgatgattt atgccaatgt gaaacaggga ttttatccca
     1981 ttcttaatgc cactgtcact gccacagttg agccagagac tggagatcct gttacgctga
     2041 gactccttga tgatggagca ggtgctgatg ttataaaaaa tgatggaatt tactcgaggt
     2101 attttttctc ctttgctgca aatggtagat atagcttgaa agtgcatgtc aatcactctc
     2161 ccagcataag caccccagcc cactctattc cagggagtca tgctatgtat gtaccaggtt
     2221 acacagcaaa cggtaatatt cagatgaatg ctccaaggaa atcagtaggc agaaatgagg
     2281 aggagcgaaa gtggggcttt agccgagtca gctcaggagg ctccttttca gtgctgggag
     2341 ttccagctgg cccccaccct gatgtgtttc caccatgcaa aattattgac ctggaagctg
     2401 taaaagtaga agaggaattg accctatctt ggacagcacc tggagaagac tttgatcagg
     2461 gccaggctac aagctatgaa ataagaatga gtaaaagtct acagaatatc caagatgact
     2521 ttaacaatgc tattttagta aatacatcaa agcgaaatcc tcagcaagct ggcatcaggg
     2581 agatatttac gttctcaccc caaatttcca cgaatggacc tgaacatcag ccaaatggag
     2641 aaacacatga aagccacaga atttatgttg caatacgagc aatggatagg aactccttac
     2701 agtctgctgt atctaacatt gcccaggcgc ctctgtttat tccccccaat tctgatcctg
     2761 tacctgccag agattatctt atattgaaag gagttttaac agcaatgggt ttgataggaa
     2821 tcatttgcct tattatagtt gtgacacatc atactttaag caggaaaaag agagcagaca
     2881 agaaagagaa tggaacaaaa ttattataaa taaatatcca aagtgtcttc cttcttagat
     2941 ataagaccca tggccttcga ctacaaaaac atactaacaa agtcaaatta acatcaaaac
     3001 tgtattaaaa tgcattgagt ttttgtacaa tacagataag atttttacat ggtagatcaa
     3061 caaattcttt ttgggggtag attagaaaac ccttacactt tggctatgaa caaataataa
     3121 aaattattct ttaaagtaat gtctttaaag gcaaagggaa gggtaaagtc ggaccagtgt
     3181 caaggaaagt ttgttttatt gaggtggaaa aatagcccca agcagagaaa aggagggtag
     3241 gtctgcatta taactgtctg tgtgaagcaa tcatttagtt actttgatta atttttcttt
     3301 tctccttatc tgtgcagaac aggttgcttg tttacaactg aagatcatgc tatattttat
     3361 atatgaagcc cctaatgcaa agctctttac ctcttgctat tttgttatat atattacaga
     3421 tgaaatctca ctgctaatgc tcagagatct tttttcactg taagaggtaa cctttaacaa
     3481 tatgggtatt acctttgtct cttcataccg gttttatgac aaaggtctat tgaatttatt
     3541 tgtttgtaag tttctactcc catcaaagca gctttctaag ttattgcctt ggttattatg
     3601 gatgatagtt atagccctta taatgcctta actaaggaag aaaagatgtt attctgagtt
     3661 tgttttaata catatatgaa catatagttt tattcaatta aaccaaagaa gaggtcagca
     3721 gggagatact aacctttgga aatgattagc tggctctgtt ttttggttaa ataagagtct
     3781 ttaatccttt ctccatcaag agttacttac caagggcagg ggaaggggga tatagaggtc
     3841 acaaggaaat aaaaatcatc tttcatcttt aattttactc cttcctctta tttttttaaa
     3901 agattatcga acaataaaat catttgcctt tttaa
//
