LOCUS       NM_001365631            7159 bp    mRNA    linear   PRI 03-OCT-2024
DEFINITION  Homo sapiens cytoplasmic linker associated protein 2 (CLASP2),
            transcript variant 7, mRNA.
ACCESSION   NM_001365631 XM_017005962
VERSION     NM_001365631.1
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7159)
  AUTHORS   Segal,D., Maier,S., Mastromarco,G.J., Qian,W.W., Nabeel-Shah,S.,
            Lee,H., Moore,G., Lacoste,J., Larsen,B., Lin,Z.Y.,
            Selvabaskaran,A., Liu,K., Smibert,C., Zhang,Z., Greenblatt,J.,
            Peng,J., Lee,H.O., Gingras,A.C. and Taipale,M.
  TITLE     A central chaperone-like role for 14-3-3 proteins in human cells
  JOURNAL   Mol Cell 83 (6), 974-993 (2023)
   PUBMED   36931259
REFERENCE   2  (bases 1 to 7159)
  AUTHORS   Rodgers,N.C., Lawrence,E.J., Sawant,A.V., Efimova,N.,
            Gonzalez-Vasquez,G., Hickman,T.T., Kaverina,I. and Zanic,M.
  TITLE     CLASP2 facilitates dynamic actin filament organization along the
            microtubule lattice
  JOURNAL   Mol Biol Cell 34 (3), br3 (2023)
   PUBMED   36598814
  REMARK    GeneRIF: CLASP2 facilitates dynamic actin filament organization
            along the microtubule lattice.
REFERENCE   3  (bases 1 to 7159)
  AUTHORS   Luo,W., Demidov,V., Shen,Q., Girao,H., Chakraborty,M., Maiorov,A.,
            Ataullakhanov,F.I., Lin,C., Maiato,H. and Grishchuk,E.L.
  TITLE     CLASP2 recognizes tubulins exposed at the microtubule plus-end in a
            nucleotide state-sensitive manner
  JOURNAL   Sci Adv 9 (1), eabq5404 (2023)
   PUBMED   36598991
  REMARK    GeneRIF: CLASP2 recognizes tubulins exposed at the microtubule
            plus-end in a nucleotide state-sensitive manner.
REFERENCE   4  (bases 1 to 7159)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   5  (bases 1 to 7159)
  AUTHORS   Chia,S., Leung,T. and Tan,I.
  TITLE     Cyclical phosphorylation of LRAP35a and CLASP2 by GSK3beta and
            CK1delta regulates EB1-dependent MT dynamics in cell migration
  JOURNAL   Cell Rep 36 (11), 109687 (2021)
   PUBMED   34525355
  REMARK    GeneRIF: Cyclical phosphorylation of LRAP35a and CLASP2 by GSK3beta
            and CK1delta regulates EB1-dependent MT dynamics in cell migration.
REFERENCE   6  (bases 1 to 7159)
  AUTHORS   Mimori-Kiyosue,Y., Grigoriev,I., Lansbergen,G., Sasaki,H.,
            Matsui,C., Severin,F., Galjart,N., Grosveld,F., Vorobjev,I.,
            Tsukita,S. and Akhmanova,A.
  TITLE     CLASP1 and CLASP2 bind to EB1 and regulate microtubule plus-end
            dynamics at the cell cortex
  JOURNAL   J Cell Biol 168 (1), 141-153 (2005)
   PUBMED   15631994
  REMARK    GeneRIF: propose that CLASP1 and CLASP2 can mediate interactions
            between microtubule plus ends and the cell cortex and act as local
            rescue factors, possibly through forming a complex with EB1 at
            microtubule tips
REFERENCE   7  (bases 1 to 7159)
  AUTHORS   Lee,H., Engel,U., Rusch,J., Scherrer,S., Sheard,K. and Van
            Vactor,D.
  TITLE     The microtubule plus end tracking protein Orbit/MAST/CLASP acts
            downstream of the tyrosine kinase Abl in mediating axon guidance
  JOURNAL   Neuron 42 (6), 913-926 (2004)
   PUBMED   15207236
REFERENCE   8  (bases 1 to 7159)
  AUTHORS   Maiato,H., Fairley,E.A., Rieder,C.L., Swedlow,J.R., Sunkel,C.E. and
            Earnshaw,W.C.
  TITLE     Human CLASP1 is an outer kinetochore component that regulates
            spindle microtubule dynamics
  JOURNAL   Cell 113 (7), 891-904 (2003)
   PUBMED   12837247
REFERENCE   9  (bases 1 to 7159)
  AUTHORS   Akhmanova,A., Hoogenraad,C.C., Drabek,K., Stepanova,T.,
            Dortland,B., Verkerk,T., Vermeulen,W., Burgering,B.M., De
            Zeeuw,C.I., Grosveld,F. and Galjart,N.
  TITLE     Clasps are CLIP-115 and -170 associating proteins involved in the
            regional regulation of microtubule dynamics in motile fibroblasts
  JOURNAL   Cell 104 (6), 923-935 (2001)
   PUBMED   11290329
REFERENCE   10 (bases 1 to 7159)
  AUTHORS   Lemos,C.L., Sampaio,P., Maiato,H., Costa,M., Omel'yanchuk,L.V.,
            Liberal,V. and Sunkel,C.E.
  TITLE     Mast, a conserved microtubule-associated protein required for
            bipolar mitotic spindle organization
  JOURNAL   EMBO J 19 (14), 3668-3682 (2000)
   PUBMED   10899121
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC113170.2, AC132515.6 and AC093114.2.
            
            On Sep 12, 2018 this sequence version replaced XM_017005962.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: mixed sample support SAMEA1965299, SAMEA1966682
                              [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000682230.1/ ENSP00000507498.1
            RefSeq Select criteria :: based on manual assertion, conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-447               AC113170.2         180343-180789       c
            448-526             AC113170.2         159390-159468       c
            527-630             AC113170.2         152364-152467       c
            631-722             AC113170.2         150812-150903       c
            723-798             AC113170.2         149595-149670       c
            799-896             AC113170.2         146894-146991       c
            897-967             AC113170.2         125980-126050       c
            968-1114            AC113170.2         107292-107438       c
            1115-1194           AC113170.2         94827-94906         c
            1195-1287           AC113170.2         89523-89615         c
            1288-1433           AC113170.2         84670-84815         c
            1434-1569           AC113170.2         82138-82273         c
            1570-1640           AC113170.2         74536-74606         c
            1641-1700           AC113170.2         71102-71161         c
            1701-1778           AC113170.2         69919-69996         c
            1779-1946           AC113170.2         69126-69293         c
            1947-2002           AC113170.2         66689-66744         c
            2003-2176           AC113170.2         65487-65660         c
            2177-2200           AC132515.6         4157-4180
            2201-2218           AC132515.6         5923-5940
            2219-2320           AC132515.6         8395-8496
            2321-2491           AC132515.6         15971-16141
            2492-2599           AC132515.6         18963-19070
            2600-2706           AC132515.6         24616-24722
            2707-2951           AC132515.6         27537-27781
            2952-3015           AC132515.6         30101-30164
            3016-3018           AC132515.6         34157-34159
            3019-3182           AC132515.6         39920-40083
            3183-3261           AC132515.6         41506-41584
            3262-3405           AC132515.6         49496-49639
            3406-3549           AC132515.6         56050-56193
            3550-3656           AC132515.6         57352-57458
            3657-3810           AC132515.6         61949-62102
            3811-4039           AC132515.6         65430-65658
            4040-4233           AC093114.2         6154-6347
            4234-4362           AC093114.2         7177-7305
            4363-4569           AC093114.2         12564-12770
            4570-4686           AC093114.2         21560-21676
            4687-7159           AC093114.2         24611-27083
FEATURES             Location/Qualifiers
     source          1..7159
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3p22.3"
     gene            1..7159
                     /gene="CLASP2"
                     /note="cytoplasmic linker associated protein 2"
                     /db_xref="GeneID:23122"
                     /db_xref="HGNC:HGNC:17078"
                     /db_xref="MIM:605853"
     exon            1..447
                     /gene="CLASP2"
                     /inference="alignment:Splign:2.1.0"
     CDS             253..4773
                     /gene="CLASP2"
                     /note="isoform 7 is encoded by transcript variant 7;
                     CLIP-associating protein 2; multiple asters (Mast)-like
                     homolog 2; protein Orbit homolog 2; CLIP-associating
                     protein CLASP2"
                     /codon_start=1
                     /product="CLIP-associating protein 2 isoform 7"
                     /protein_id="NP_001352560.1"
                     /db_xref="CCDS:CCDS93235.1"
                     /db_xref="GeneID:23122"
                     /db_xref="HGNC:HGNC:17078"
                     /db_xref="MIM:605853"
                     /translation="MEPRSMEYFCAQVQQKDVGGRLQVGQELLLYLGAPGAISDLEED
                     LGRLGKTVDALTGWVGSSNYRVSLMGLEILSAFVDRLSTRFKSYVAMVIVALIDRMGD
                     AKDKVRDEAQTLILKLMDQVAPPMYIWEQLASGFKHKNFRSREGVCLCLIETLNIFGA
                     QPLVISKLIPHLCILFGDSNSQVRDAAILAIVEIYRHVGEKVRMDLYKRGIPPARLEM
                     IFAKFDEVQSSGGMILSVCKDKSFDDEESVDGNRPSSAASAFKVPAPKTSGNPANSAR
                     KPGSAGGPKVGGASKEGGAGAVDEDDFIKAFTDVPSIQIYSSRELEETLNKIREILSD
                     DKHDWDQRANALKKIRSLLVAGAAQYDCFFQHLRLLDGALKLSAKDLRSQVVREACIT
                     VAHLSTVLGNKFDHGAEAIVPTLFNLVPNSAKVMATSGCAAIRFIIRHTHVPRLIPLI
                     TSNCTSKSVPVRRRSFEFLDLLLQEWQTHSLERHAAVLVETIKKGIHDADAEARVEAR
                     KTYMGLRNHFPGEAETLYNSLEPSYQKSLQTYLKSSGSVASLPQSDRSSSSSQESLNR
                     PFSSKWSTANPSTVAGRVSAGSSKASSLPGSLQRSRSDIDVNAAAGAKAHHAAGQSVR
                     SGRLGAGALNAGSYASLEDTSDKLDGTASEDGRVRAKLSAPLAGMGNAKADSRGRSRT
                     KMVSQSQPGSRSGSPGRVLTTTALSTVSSGVQRVLVNSASAQKRSKIPRSQGCSREAS
                     PSRLSVARSSRIPRPSVSQGCSREASRESSRDTSPVRSFQPLGPGYGISQSSRLSSSV
                     SAMRVLNTGSDVEEAVADALKKPARRRYESYGMHSDDDANSDASSACSERSYSSRNGS
                     IPTYMRQTEDVAEVLNRCASSNWSERKEGLLGLQNLLKNQRTLSRVELKRLCEIFTRM
                     FADPHGKRVFSMFLETLVDFIQVHKDDLQDWLFVLLTQLLKKMGADLLGSVQAKVQKA
                     LDVTRESFPNDLQFNILMRFTVDQTQTPSLKVKVAILKYIETLAKQMDPGDFINSSET
                     RLAVSRVITWTTEPKSSDVRKAAQSVLISLFELNTPEFTMLLGALPKTFQDGATKLLH
                     NHLRNTGNGTQSSMGSPLTRPTPRSPANWSSPLTSPTNTSQNTLSPSAFDYDTENMNS
                     EDIYSSLRGVTEAIQNFSFRSQEDMNEPLKRDSKKDDGDSMCGGPGMSDPRAGGDATD
                     SSQTALDNKASLLHSMPTHSSPRSRDYNPYNYSDSISPFNKSALKEAMFDDDADQFPD
                     DLSLDHSDLVAELLKELSNHNERVEERKIALYELMKLTQEESFSVWDEHFKTILLLLL
                     ETLGDKEPTIRALALKVLREILRHQPARFKNYAELTVMKTLEAHKDPHKEVVRSAEEA
                     ASVLATSISPEQCIKVLCPIIQTADYPINLAAIKMQTKVIERVSKETLNLLLPEIMPG
                     LIQGYDNSESSVRKACVFCLVAVHAVIGDELKPHLSQLTGSKMKLLNLYIKRAQTGSG
                     GADPTTDVSGQS"
     exon            448..526
                     /gene="CLASP2"
                     /inference="alignment:Splign:2.1.0"
     exon            527..630
                     /gene="CLASP2"
                     /inference="alignment:Splign:2.1.0"
     exon            631..722
                     /gene="CLASP2"
                     /inference="alignment:Splign:2.1.0"
     exon            723..798
                     /gene="CLASP2"
                     /inference="alignment:Splign:2.1.0"
     exon            799..896
                     /gene="CLASP2"
                     /inference="alignment:Splign:2.1.0"
     exon            897..967
                     /gene="CLASP2"
                     /inference="alignment:Splign:2.1.0"
     exon            968..1114
                     /gene="CLASP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1115..1194
                     /gene="CLASP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1195..1287
                     /gene="CLASP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1288..1433
                     /gene="CLASP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1434..1569
                     /gene="CLASP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1570..1640
                     /gene="CLASP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1641..1700
                     /gene="CLASP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1701..1778
                     /gene="CLASP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1779..1946
                     /gene="CLASP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1947..2002
                     /gene="CLASP2"
                     /inference="alignment:Splign:2.1.0"
     exon            2003..2176
                     /gene="CLASP2"
                     /inference="alignment:Splign:2.1.0"
     exon            2177..2200
                     /gene="CLASP2"
                     /inference="alignment:Splign:2.1.0"
     exon            2201..2218
                     /gene="CLASP2"
                     /inference="alignment:Splign:2.1.0"
     exon            2219..2320
                     /gene="CLASP2"
                     /inference="alignment:Splign:2.1.0"
     exon            2321..2491
                     /gene="CLASP2"
                     /inference="alignment:Splign:2.1.0"
     exon            2492..2599
                     /gene="CLASP2"
                     /inference="alignment:Splign:2.1.0"
     exon            2600..2706
                     /gene="CLASP2"
                     /inference="alignment:Splign:2.1.0"
     exon            2707..2951
                     /gene="CLASP2"
                     /inference="alignment:Splign:2.1.0"
     exon            2952..3015
                     /gene="CLASP2"
                     /inference="alignment:Splign:2.1.0"
     exon            3016..3018
                     /gene="CLASP2"
                     /inference="alignment:Splign:2.1.0"
     exon            3019..3182
                     /gene="CLASP2"
                     /inference="alignment:Splign:2.1.0"
     exon            3183..3261
                     /gene="CLASP2"
                     /inference="alignment:Splign:2.1.0"
     exon            3262..3405
                     /gene="CLASP2"
                     /inference="alignment:Splign:2.1.0"
     exon            3406..3549
                     /gene="CLASP2"
                     /inference="alignment:Splign:2.1.0"
     exon            3550..3656
                     /gene="CLASP2"
                     /inference="alignment:Splign:2.1.0"
     exon            3657..3810
                     /gene="CLASP2"
                     /inference="alignment:Splign:2.1.0"
     exon            3811..4039
                     /gene="CLASP2"
                     /inference="alignment:Splign:2.1.0"
     exon            4040..4233
                     /gene="CLASP2"
                     /inference="alignment:Splign:2.1.0"
     exon            4234..4362
                     /gene="CLASP2"
                     /inference="alignment:Splign:2.1.0"
     exon            4363..4569
                     /gene="CLASP2"
                     /inference="alignment:Splign:2.1.0"
     exon            4570..4686
                     /gene="CLASP2"
                     /inference="alignment:Splign:2.1.0"
     exon            4687..7159
                     /gene="CLASP2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6378..6383
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CLASP2"
                     /note="hexamer: AATAAA"
     polyA_site      6413
                     /gene="CLASP2"
                     /note="major polyA site"
     regulatory      7131..7136
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CLASP2"
                     /note="hexamer: ATTAAA"
     regulatory      7137..7142
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CLASP2"
                     /note="hexamer: ATTATA"
     polyA_site      7149
                     /gene="CLASP2"
     polyA_site      7159
                     /gene="CLASP2"
                     /note="major polyA site"
ORIGIN      
        1 acgcgcgtgc tgacgtcgcc cgcggccgcg gcctctgaag cgggctgggg atcggggggc
       61 gccgagtttg actagtttgg gggcggctgg gcgcttggcg ttcctcccgc cgcccgctgc
      121 gccccgcaag ccgcgcccct ggcgggctaa gtgagtcccg cccgctcccg cggggacccg
      181 cactggaggc tgggcggctc tcggcgaaag ttggccgctc acagactggc aggcgggcgg
      241 gcggccgcag ccatggagcc ccgcagcatg gagtacttct gcgcccaggt gcagcagaag
      301 gacgtcggcg gccggctgca ggtcggccag gagctcctgc tctaccttgg cgcccccggc
      361 gccatctcgg acctggagga ggacctgggc cgcctaggca agacagtcga cgcgctcacc
      421 ggctgggtgg gttcgagcaa ctaccgggta tcattaatgg gattggaaat tttaagtgcc
      481 tttgtggaca gattatcaac acgctttaaa tcctatgtag caatggttat tgtagcttta
      541 atagacagaa tgggagatgc caaagacaag gttcgagatg aagctcagac tctgatattg
      601 aagttaatgg atcaagtagc accacctatg tacatttggg agcagttggc ttctggtttt
      661 aaacacaaga attttcgatc tcgagaaggc gtgtgtctgt gtcttattga aaccttaaac
      721 atttttgggg ctcagccact agtcatcagc aaattgatac cacatttgtg tatcctgttt
      781 ggagactcca acagtcaggt gagagatgct gcaatattgg ctatagtgga gatttataga
      841 catgtgggag aaaaagtgag gatggatctt tataagagag gaattccccc tgctagatta
      901 gaaatgatat ttgccaaatt tgatgaagtg caaagttcag gcggtatgat tttgagtgtc
      961 tgcaaagata aaagcttcga tgatgaagaa tcagtggatg gaaataggcc atcatcagct
     1021 gcatcagcct tcaaggttcc tgcacctaaa acatccggaa atcctgccaa cagtgcaagg
     1081 aagcctggtt cagcaggtgg ccctaaggtt ggaggtgctt ctaaggaagg aggtgctgga
     1141 gcagttgatg aagatgattt tataaaagct tttacagatg tcccttctat tcagatttat
     1201 tctagtcgag aactcgaaga aacattaaat aaaatcaggg aaattttgtc agatgataaa
     1261 catgactggg atcagcgtgc caatgcactg aagaaaattc gatcactgct tgttgctgga
     1321 gctgcacagt atgattgctt ttttcaacat ttacgattgt tggatggagc acttaaactt
     1381 tcagctaagg atcttagatc ccaggtggtt agagaagctt gtattactgt agcccacctt
     1441 tcaacagttt tgggaaacaa gtttgatcat ggcgctgaag ccattgtacc tacacttttt
     1501 aatctcgtcc ccaatagtgc aaaagtcatg gcaacttctg gatgtgcagc aatcagattt
     1561 atcattcggc atactcatgt acccagactt atacctttaa taacaagcaa ttgcacatca
     1621 aaatcagttc ccgtgaggag acgttcattt gaatttttag atttattgtt gcaagagtgg
     1681 cagactcatt cattggaaag acatgcagcc gtcttggttg aaactattaa aaagggaatt
     1741 catgatgctg acgctgaggc cagagtggag gcaagaaaga catacatggg tcttagaaac
     1801 cactttcctg gtgaagctga aacattatat aattcccttg agccatctta tcagaagagt
     1861 cttcaaactt acttaaagag ttctggcagt gtagcatctc ttccacaatc agacaggtcc
     1921 tcatccagct cacaggaaag tctcaatcgc cctttttctt ccaaatggtc tacagcaaat
     1981 ccatcaactg tggctggaag agtatcagca ggcagcagca aagccagttc ccttccagga
     2041 agcctgcagc gttcacgaag tgacattgat gtgaatgctg ctgcaggtgc caaggcacat
     2101 catgctgctg gacagtctgt gcgaagcggg cgcttaggtg caggtgccct gaatgcaggt
     2161 tcctatgcgt cactagagga tacttctgac aagctggatg gaacagcatc tgaagatggc
     2221 cgggtgagag caaaactttc agcaccactt gctggcatgg gaaatgccaa ggcagattct
     2281 agaggaagaa gtcgaacaaa aatggtgtct caatcacagc ctggtagccg gtctgggtct
     2341 ccaggaagag ttctgaccac aacagccctg tccactgtga gctctggtgt tcaaagagtc
     2401 ctggtcaatt cagcctcagc acaaaaaaga agcaagatac cacggagcca gggctgtagc
     2461 agagaggcta gtccatctag gctttcagtg gcccgaagca gtcgtattcc tcgaccaagt
     2521 gtgagtcaag gatgcagccg ggaagctagt cgggagagca gcagagacac aagtcctgtt
     2581 cgctcttttc agcccctcgg tccaggttat gggatcagcc aatcaagtcg actgtcgtct
     2641 tctgttagtg ccatgcgagt cctgaacaca ggttctgatg tggaggaggc ggtggcagat
     2701 gccttgaaaa aaccagctcg aagaagatat gaatcatatg gaatgcattc agatgatgac
     2761 gccaacagcg atgcatctag tgcttgttca gaacgctcct atagttctcg aaatggtagt
     2821 attcctacat atatgaggca gacggaagat gtggcagaag tcctcaatag atgtgctagt
     2881 tccaattggt cagaaaggaa agaaggcctc ctaggtctgc agaacttatt aaaaaatcag
     2941 agaacactaa gtcgagttga actgaaaaga ttatgtgaaa ttttcacaag aatgtttgct
     3001 gaccctcatg gcaagagagt attcagcatg tttttggaga ctctagtgga tttcatacaa
     3061 gtccacaaag atgatcttca agattggttg tttgtactgc tgacacaact actaaaaaaa
     3121 atgggtgctg atttgcttgg atctgttcag gcaaaagttc agaaagccct tgatgttaca
     3181 agagagtctt ttccaaatga tcttcagttc aatattctaa tgagatttac agttgatcag
     3241 acccagacac caagcttaaa ggtgaaggtt gctatcctta aatacataga aactctggcc
     3301 aaacagatgg atccaggaga ttttataaat tccagtgaaa ctcgcctagc agtgtctcgg
     3361 gtcatcactt ggacaacaga acccaaaagt tctgatgttc ggaaggcagc acagtcagtg
     3421 ctgatttcat tatttgaact caatacccca gagtttacaa tgttattagg agctttacca
     3481 aaaacttttc aggatggtgc taccaagctt cttcataatc accttcgaaa cactggcaat
     3541 ggaacccaga gttccatggg gagtcctttg acaagaccaa caccacgatc accagctaac
     3601 tggtccagtc ctcttacttc tcctaccaat acatcacaga atactttatc tccaagtgca
     3661 tttgattatg acacagaaaa tatgaactct gaagatattt atagctctct tagaggtgtc
     3721 actgaagcaa tccagaattt cagcttccgt agccaagaag atatgaatga gccattgaaa
     3781 agggattcta aaaaagatga tggcgattca atgtgtggtg gtcctgggat gtctgaccca
     3841 agagcaggag gtgatgctac tgactcaagt caaacagctc ttgataataa agcttcattg
     3901 ctccattcaa tgcctactca ctcctctcca cgctctcgag actataatcc atataactat
     3961 tcagatagca tcagtccctt caacaagtct gccctcaagg aagccatgtt tgatgatgat
     4021 gctgaccagt ttcctgacga tctttcccta gatcattctg acctagttgc agagttgttg
     4081 aaggagctgt ctaaccataa tgagcgtgta gaagaaagaa aaattgccct ctatgaactt
     4141 atgaaactga cacaggaaga atcttttagt gtttgggatg aacacttcaa aacaatattg
     4201 cttttattgc ttgaaacgct tggagataaa gagcctacaa tcagggcttt ggcattaaag
     4261 gttttaagag aaatcctaag gcatcaacca gcaagattta aaaactatgc agaattgact
     4321 gtcatgaaaa cattggaagc acataaagat cctcataagg aggtggtgag atctgctgag
     4381 gaagcggcat cagtgttggc cacttcaatt agtccagagc agtgcatcaa agtgctttgt
     4441 cctatcattc aaactgcaga ctacccaatt aatctggctg caatcaaaat gcaaacaaaa
     4501 gtgatagaga gagtgtccaa ggaaacccta aacctgcttt tgccagagat tatgccaggt
     4561 ctaatacagg gttatgataa ttcagagagc agtgttcgga aagcttgtgt cttctgcctg
     4621 gtggctgttc atgcggtaat tggtgatgaa ctaaaaccac atctcagtca acttactggc
     4681 agtaaaatga agctactgaa tctttacatc aaacgtgcac aaacaggttc tggaggagct
     4741 gatcccacta ctgatgtttc tggacaaagt tagtgaagct catcacagcg aaccaggtct
     4801 ctcaaaagaa aggacagata gaccaccctc atcaatgaaa ggaagttctc aaacacatcc
     4861 tttggaactt actattgttt cccagtttta gttttttgtt tcgtttcgtt ttgtattttc
     4921 tgtaacagag gactatcctc agtctgcatg taacttttat gatagttatt ccaaattcaa
     4981 gaagaagcag tattaacatc aattgatcga cacaaagtaa tttttaattt aattcatcat
     5041 ttcacatgtt tgtactttgt cttcccatta acctttgcca gtgttatgat tgtataaatt
     5101 tttttaaatg ctggttaaac aggaatgctt aaagctttaa aagtttaaca gtctaaaaca
     5161 tttttgcttt tattcaactg cagaataata tttttattgc tactttgagt tttgtttcgt
     5221 atcatgtcct atgctagaaa tatttaaatg atgtgaaaca aagcaggact aatttgaact
     5281 acagctggac tccgtttgtg tgatggtgat acatgtcatt agttgcaact tctttggggt
     5341 gatctatagt ttgaaaacta aaacctcaaa gacagatgtt acagaatcag ccagttctgt
     5401 aaaactgata ttgtctattg gttattgatc ttgccatctt tatttaaaac catgtccctt
     5461 ctatgatccc ttaagaaagc tgcaccaaat catctgcctg ttttttcttg atacttactg
     5521 aaatagaagg ttttattgca gggtttattt tggtttgttt atatctttgt tgtgaatgat
     5581 gcttttttgt atttattaat atcaaattca cttatgaata aacttgataa tggaaacgga
     5641 caaaaaaaat caagtgcgtg tgtgtccttg accgtcttct gtttctcacg taataaacaa
     5701 attatcgaga catgggagtg accagcacct tttctttaaa tggtggaacc tggtttcctt
     5761 ttaccatgaa attgtcttac ttgaaaatat tgatcctgat gagagagaag atggtgccaa
     5821 ggctgtcttt gtataatggg ctcaaattct ctacctcttc agggctaata cttttaactg
     5881 agctgctgcc tatagtgtct tttggaaaac tacttaaagg gtgattttct gttacttttt
     5941 agcaaatttt tttaatcacc tcttgctaca cccattcttt tcatgtgcag ccgactcaaa
     6001 aattaccagt tttggtgaaa ggctaaatta gataatttgg aaccaggata ctaatgattt
     6061 ctcatcttta ctttttttta atcctaatat aaagtgaatt tgattgaaaa ggcaaatagc
     6121 tattagggaa gcagtttgcc attgttgcag agttatctgt actttgttta actgaaaaaa
     6181 atgtagaaat atatgtaaag aatttaagac aagagtactg aatggatgat ttgtcatagg
     6241 ctttcccctt tctttctgtt ctagcagcag gaaaagtttc tctatatcct ctccctctac
     6301 ctgtaacaat tttgttttct actgttaatt acattgtgta tttatagttc tatgcttact
     6361 gttgtgcata tactggcaat aaaactgtac ataacattac ttgaaaaagt taataatgta
     6421 tatcagtttt tctgtctcac tgtgtaacaa gtcactcagt tttattttaa ctttagacgg
     6481 tcttgtatca gtggtggtct cttgaatttt gtaagttcat ctgaggagaa aagatttttc
     6541 aggtgtagct accacaatca aaggtatata gctacatacg catgtatata ttacagctta
     6601 tctgtaagaa gaaaatgcat tttaaacaca actcttctca gtagcatttt atgacctttg
     6661 gatatgtttg taatcatttc gaatcaaaat attgatttaa ttttgacctc tggtttaaga
     6721 tactgcttta actactgttg acaaccaagt agagtgactt aagctgaaca gtaactaact
     6781 ggaaaattag ataagcacct ggcatctaat ggcaggcagg cactcaagaa atgaattaac
     6841 tacataatgg aaaagtatgg tttaatgtgt ccaaatgaaa gctagtagat gtaaacatgg
     6901 aaaaattgtg tttacaattt tataatctca gttgataaga ctataagaaa gctgattatt
     6961 taaatcacta tatacaatac acccttaatt tgttcattcc agaaacatac tgagatgtca
     7021 gctacttaaa aatggtcaca aaaagctact gtttatattt ttcctcctgc tattctctcc
     7081 caaattaatt attaataagt gttgttcatt tactgcactg ctgagaacta attaaaatta
     7141 tatattccag attgtaaaa
//
