LOCUS       NM_001365629            7243 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens cytoplasmic linker associated protein 2 (CLASP2),
            transcript variant 5, mRNA.
ACCESSION   NM_001365629 XM_017005953
VERSION     NM_001365629.1
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7243)
  AUTHORS   Segal,D., Maier,S., Mastromarco,G.J., Qian,W.W., Nabeel-Shah,S.,
            Lee,H., Moore,G., Lacoste,J., Larsen,B., Lin,Z.Y.,
            Selvabaskaran,A., Liu,K., Smibert,C., Zhang,Z., Greenblatt,J.,
            Peng,J., Lee,H.O., Gingras,A.C. and Taipale,M.
  TITLE     A central chaperone-like role for 14-3-3 proteins in human cells
  JOURNAL   Mol Cell 83 (6), 974-993 (2023)
   PUBMED   36931259
REFERENCE   2  (bases 1 to 7243)
  AUTHORS   Rodgers,N.C., Lawrence,E.J., Sawant,A.V., Efimova,N.,
            Gonzalez-Vasquez,G., Hickman,T.T., Kaverina,I. and Zanic,M.
  TITLE     CLASP2 facilitates dynamic actin filament organization along the
            microtubule lattice
  JOURNAL   Mol Biol Cell 34 (3), br3 (2023)
   PUBMED   36598814
  REMARK    GeneRIF: CLASP2 facilitates dynamic actin filament organization
            along the microtubule lattice.
REFERENCE   3  (bases 1 to 7243)
  AUTHORS   Luo,W., Demidov,V., Shen,Q., Girao,H., Chakraborty,M., Maiorov,A.,
            Ataullakhanov,F.I., Lin,C., Maiato,H. and Grishchuk,E.L.
  TITLE     CLASP2 recognizes tubulins exposed at the microtubule plus-end in a
            nucleotide state-sensitive manner
  JOURNAL   Sci Adv 9 (1), eabq5404 (2023)
   PUBMED   36598991
  REMARK    GeneRIF: CLASP2 recognizes tubulins exposed at the microtubule
            plus-end in a nucleotide state-sensitive manner.
REFERENCE   4  (bases 1 to 7243)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   5  (bases 1 to 7243)
  AUTHORS   Chia,S., Leung,T. and Tan,I.
  TITLE     Cyclical phosphorylation of LRAP35a and CLASP2 by GSK3beta and
            CK1delta regulates EB1-dependent MT dynamics in cell migration
  JOURNAL   Cell Rep 36 (11), 109687 (2021)
   PUBMED   34525355
  REMARK    GeneRIF: Cyclical phosphorylation of LRAP35a and CLASP2 by GSK3beta
            and CK1delta regulates EB1-dependent MT dynamics in cell migration.
REFERENCE   6  (bases 1 to 7243)
  AUTHORS   Mimori-Kiyosue,Y., Grigoriev,I., Lansbergen,G., Sasaki,H.,
            Matsui,C., Severin,F., Galjart,N., Grosveld,F., Vorobjev,I.,
            Tsukita,S. and Akhmanova,A.
  TITLE     CLASP1 and CLASP2 bind to EB1 and regulate microtubule plus-end
            dynamics at the cell cortex
  JOURNAL   J Cell Biol 168 (1), 141-153 (2005)
   PUBMED   15631994
  REMARK    GeneRIF: propose that CLASP1 and CLASP2 can mediate interactions
            between microtubule plus ends and the cell cortex and act as local
            rescue factors, possibly through forming a complex with EB1 at
            microtubule tips
REFERENCE   7  (bases 1 to 7243)
  AUTHORS   Lee,H., Engel,U., Rusch,J., Scherrer,S., Sheard,K. and Van
            Vactor,D.
  TITLE     The microtubule plus end tracking protein Orbit/MAST/CLASP acts
            downstream of the tyrosine kinase Abl in mediating axon guidance
  JOURNAL   Neuron 42 (6), 913-926 (2004)
   PUBMED   15207236
REFERENCE   8  (bases 1 to 7243)
  AUTHORS   Maiato,H., Fairley,E.A., Rieder,C.L., Swedlow,J.R., Sunkel,C.E. and
            Earnshaw,W.C.
  TITLE     Human CLASP1 is an outer kinetochore component that regulates
            spindle microtubule dynamics
  JOURNAL   Cell 113 (7), 891-904 (2003)
   PUBMED   12837247
REFERENCE   9  (bases 1 to 7243)
  AUTHORS   Akhmanova,A., Hoogenraad,C.C., Drabek,K., Stepanova,T.,
            Dortland,B., Verkerk,T., Vermeulen,W., Burgering,B.M., De
            Zeeuw,C.I., Grosveld,F. and Galjart,N.
  TITLE     Clasps are CLIP-115 and -170 associating proteins involved in the
            regional regulation of microtubule dynamics in motile fibroblasts
  JOURNAL   Cell 104 (6), 923-935 (2001)
   PUBMED   11290329
REFERENCE   10 (bases 1 to 7243)
  AUTHORS   Lemos,C.L., Sampaio,P., Maiato,H., Costa,M., Omel'yanchuk,L.V.,
            Liberal,V. and Sunkel,C.E.
  TITLE     Mast, a conserved microtubule-associated protein required for
            bipolar mitotic spindle organization
  JOURNAL   EMBO J 19 (14), 3668-3682 (2000)
   PUBMED   10899121
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC113170.2, AC132515.6 and AC093114.2.
            
            On Sep 12, 2018 this sequence version replaced XM_017005953.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803613.285403.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-447               AC113170.2         180343-180789       c
            448-526             AC113170.2         159390-159468       c
            527-630             AC113170.2         152364-152467       c
            631-722             AC113170.2         150812-150903       c
            723-798             AC113170.2         149595-149670       c
            799-896             AC113170.2         146894-146991       c
            897-967             AC113170.2         125980-126050       c
            968-1114            AC113170.2         107292-107438       c
            1115-1194           AC113170.2         94827-94906         c
            1195-1287           AC113170.2         89523-89615         c
            1288-1433           AC113170.2         84670-84815         c
            1434-1569           AC113170.2         82138-82273         c
            1570-1640           AC113170.2         74536-74606         c
            1641-1700           AC113170.2         71102-71161         c
            1701-1778           AC113170.2         69919-69996         c
            1779-1946           AC113170.2         69126-69293         c
            1947-2002           AC113170.2         66689-66744         c
            2003-2176           AC113170.2         65487-65660         c
            2177-2200           AC132515.6         4157-4180
            2201-2218           AC132515.6         5923-5940
            2219-2320           AC132515.6         8395-8496
            2321-2491           AC132515.6         15971-16141
            2492-2599           AC132515.6         18963-19070
            2600-2662           AC132515.6         23627-23689
            2663-2769           AC132515.6         24616-24722
            2770-2793           AC132515.6         26395-26418
            2794-3038           AC132515.6         27537-27781
            3039-3102           AC132515.6         30101-30164
            3103-3266           AC132515.6         39920-40083
            3267-3345           AC132515.6         41506-41584
            3346-3489           AC132515.6         49496-49639
            3490-3633           AC132515.6         56050-56193
            3634-3740           AC132515.6         57352-57458
            3741-3894           AC132515.6         61949-62102
            3895-4123           AC132515.6         65430-65658
            4124-4317           AC093114.2         6154-6347
            4318-4446           AC093114.2         7177-7305
            4447-4653           AC093114.2         12564-12770
            4654-4770           AC093114.2         21560-21676
            4771-7243           AC093114.2         24611-27083
FEATURES             Location/Qualifiers
     source          1..7243
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3p22.3"
     gene            1..7243
                     /gene="CLASP2"
                     /note="cytoplasmic linker associated protein 2"
                     /db_xref="GeneID:23122"
                     /db_xref="HGNC:HGNC:17078"
                     /db_xref="MIM:605853"
     exon            1..447
                     /gene="CLASP2"
                     /inference="alignment:Splign:2.1.0"
     CDS             253..4857
                     /gene="CLASP2"
                     /note="isoform 5 is encoded by transcript variant 5;
                     CLIP-associating protein 2; multiple asters (Mast)-like
                     homolog 2; protein Orbit homolog 2; CLIP-associating
                     protein CLASP2"
                     /codon_start=1
                     /product="CLIP-associating protein 2 isoform 5"
                     /protein_id="NP_001352558.1"
                     /db_xref="GeneID:23122"
                     /db_xref="HGNC:HGNC:17078"
                     /db_xref="MIM:605853"
                     /translation="MEPRSMEYFCAQVQQKDVGGRLQVGQELLLYLGAPGAISDLEED
                     LGRLGKTVDALTGWVGSSNYRVSLMGLEILSAFVDRLSTRFKSYVAMVIVALIDRMGD
                     AKDKVRDEAQTLILKLMDQVAPPMYIWEQLASGFKHKNFRSREGVCLCLIETLNIFGA
                     QPLVISKLIPHLCILFGDSNSQVRDAAILAIVEIYRHVGEKVRMDLYKRGIPPARLEM
                     IFAKFDEVQSSGGMILSVCKDKSFDDEESVDGNRPSSAASAFKVPAPKTSGNPANSAR
                     KPGSAGGPKVGGASKEGGAGAVDEDDFIKAFTDVPSIQIYSSRELEETLNKIREILSD
                     DKHDWDQRANALKKIRSLLVAGAAQYDCFFQHLRLLDGALKLSAKDLRSQVVREACIT
                     VAHLSTVLGNKFDHGAEAIVPTLFNLVPNSAKVMATSGCAAIRFIIRHTHVPRLIPLI
                     TSNCTSKSVPVRRRSFEFLDLLLQEWQTHSLERHAAVLVETIKKGIHDADAEARVEAR
                     KTYMGLRNHFPGEAETLYNSLEPSYQKSLQTYLKSSGSVASLPQSDRSSSSSQESLNR
                     PFSSKWSTANPSTVAGRVSAGSSKASSLPGSLQRSRSDIDVNAAAGAKAHHAAGQSVR
                     SGRLGAGALNAGSYASLEDTSDKLDGTASEDGRVRAKLSAPLAGMGNAKADSRGRSRT
                     KMVSQSQPGSRSGSPGRVLTTTALSTVSSGVQRVLVNSASAQKRSKIPRSQGCSREAS
                     PSRLSVARSSRIPRPSVSQGCSREASRESSRDTSPVRSFQPLASRHHSRSTGALYAPE
                     VYGASGPGYGISQSSRLSSSVSAMRVLNTGSDVEEAVADALLLGDIRTKKKPARRRYE
                     SYGMHSDDDANSDASSACSERSYSSRNGSIPTYMRQTEDVAEVLNRCASSNWSERKEG
                     LLGLQNLLKNQRTLSRVELKRLCEIFTRMFADPHGKVFSMFLETLVDFIQVHKDDLQD
                     WLFVLLTQLLKKMGADLLGSVQAKVQKALDVTRESFPNDLQFNILMRFTVDQTQTPSL
                     KVKVAILKYIETLAKQMDPGDFINSSETRLAVSRVITWTTEPKSSDVRKAAQSVLISL
                     FELNTPEFTMLLGALPKTFQDGATKLLHNHLRNTGNGTQSSMGSPLTRPTPRSPANWS
                     SPLTSPTNTSQNTLSPSAFDYDTENMNSEDIYSSLRGVTEAIQNFSFRSQEDMNEPLK
                     RDSKKDDGDSMCGGPGMSDPRAGGDATDSSQTALDNKASLLHSMPTHSSPRSRDYNPY
                     NYSDSISPFNKSALKEAMFDDDADQFPDDLSLDHSDLVAELLKELSNHNERVEERKIA
                     LYELMKLTQEESFSVWDEHFKTILLLLLETLGDKEPTIRALALKVLREILRHQPARFK
                     NYAELTVMKTLEAHKDPHKEVVRSAEEAASVLATSISPEQCIKVLCPIIQTADYPINL
                     AAIKMQTKVIERVSKETLNLLLPEIMPGLIQGYDNSESSVRKACVFCLVAVHAVIGDE
                     LKPHLSQLTGSKMKLLNLYIKRAQTGSGGADPTTDVSGQS"
     exon            448..526
                     /gene="CLASP2"
                     /inference="alignment:Splign:2.1.0"
     exon            527..630
                     /gene="CLASP2"
                     /inference="alignment:Splign:2.1.0"
     exon            631..722
                     /gene="CLASP2"
                     /inference="alignment:Splign:2.1.0"
     exon            723..798
                     /gene="CLASP2"
                     /inference="alignment:Splign:2.1.0"
     exon            799..896
                     /gene="CLASP2"
                     /inference="alignment:Splign:2.1.0"
     exon            897..967
                     /gene="CLASP2"
                     /inference="alignment:Splign:2.1.0"
     exon            968..1114
                     /gene="CLASP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1115..1194
                     /gene="CLASP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1195..1287
                     /gene="CLASP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1288..1433
                     /gene="CLASP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1434..1569
                     /gene="CLASP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1570..1640
                     /gene="CLASP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1641..1700
                     /gene="CLASP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1701..1778
                     /gene="CLASP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1779..1946
                     /gene="CLASP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1947..2002
                     /gene="CLASP2"
                     /inference="alignment:Splign:2.1.0"
     exon            2003..2176
                     /gene="CLASP2"
                     /inference="alignment:Splign:2.1.0"
     exon            2177..2200
                     /gene="CLASP2"
                     /inference="alignment:Splign:2.1.0"
     exon            2201..2218
                     /gene="CLASP2"
                     /inference="alignment:Splign:2.1.0"
     exon            2219..2320
                     /gene="CLASP2"
                     /inference="alignment:Splign:2.1.0"
     exon            2321..2491
                     /gene="CLASP2"
                     /inference="alignment:Splign:2.1.0"
     exon            2492..2599
                     /gene="CLASP2"
                     /inference="alignment:Splign:2.1.0"
     exon            2600..2662
                     /gene="CLASP2"
                     /inference="alignment:Splign:2.1.0"
     exon            2663..2769
                     /gene="CLASP2"
                     /inference="alignment:Splign:2.1.0"
     exon            2770..2793
                     /gene="CLASP2"
                     /inference="alignment:Splign:2.1.0"
     exon            2794..3038
                     /gene="CLASP2"
                     /inference="alignment:Splign:2.1.0"
     exon            3039..3102
                     /gene="CLASP2"
                     /inference="alignment:Splign:2.1.0"
     exon            3103..3266
                     /gene="CLASP2"
                     /inference="alignment:Splign:2.1.0"
     exon            3267..3345
                     /gene="CLASP2"
                     /inference="alignment:Splign:2.1.0"
     exon            3346..3489
                     /gene="CLASP2"
                     /inference="alignment:Splign:2.1.0"
     exon            3490..3633
                     /gene="CLASP2"
                     /inference="alignment:Splign:2.1.0"
     exon            3634..3740
                     /gene="CLASP2"
                     /inference="alignment:Splign:2.1.0"
     exon            3741..3894
                     /gene="CLASP2"
                     /inference="alignment:Splign:2.1.0"
     exon            3895..4123
                     /gene="CLASP2"
                     /inference="alignment:Splign:2.1.0"
     exon            4124..4317
                     /gene="CLASP2"
                     /inference="alignment:Splign:2.1.0"
     exon            4318..4446
                     /gene="CLASP2"
                     /inference="alignment:Splign:2.1.0"
     exon            4447..4653
                     /gene="CLASP2"
                     /inference="alignment:Splign:2.1.0"
     exon            4654..4770
                     /gene="CLASP2"
                     /inference="alignment:Splign:2.1.0"
     exon            4771..7243
                     /gene="CLASP2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6462..6467
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CLASP2"
                     /note="hexamer: AATAAA"
     polyA_site      6497
                     /gene="CLASP2"
                     /note="major polyA site"
     regulatory      7215..7220
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CLASP2"
                     /note="hexamer: ATTAAA"
     regulatory      7221..7226
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CLASP2"
                     /note="hexamer: ATTATA"
     polyA_site      7233
                     /gene="CLASP2"
     polyA_site      7243
                     /gene="CLASP2"
                     /note="major polyA site"
ORIGIN      
        1 acgcgcgtgc tgacgtcgcc cgcggccgcg gcctctgaag cgggctgggg atcggggggc
       61 gccgagtttg actagtttgg gggcggctgg gcgcttggcg ttcctcccgc cgcccgctgc
      121 gccccgcaag ccgcgcccct ggcgggctaa gtgagtcccg cccgctcccg cggggacccg
      181 cactggaggc tgggcggctc tcggcgaaag ttggccgctc acagactggc aggcgggcgg
      241 gcggccgcag ccatggagcc ccgcagcatg gagtacttct gcgcccaggt gcagcagaag
      301 gacgtcggcg gccggctgca ggtcggccag gagctcctgc tctaccttgg cgcccccggc
      361 gccatctcgg acctggagga ggacctgggc cgcctaggca agacagtcga cgcgctcacc
      421 ggctgggtgg gttcgagcaa ctaccgggta tcattaatgg gattggaaat tttaagtgcc
      481 tttgtggaca gattatcaac acgctttaaa tcctatgtag caatggttat tgtagcttta
      541 atagacagaa tgggagatgc caaagacaag gttcgagatg aagctcagac tctgatattg
      601 aagttaatgg atcaagtagc accacctatg tacatttggg agcagttggc ttctggtttt
      661 aaacacaaga attttcgatc tcgagaaggc gtgtgtctgt gtcttattga aaccttaaac
      721 atttttgggg ctcagccact agtcatcagc aaattgatac cacatttgtg tatcctgttt
      781 ggagactcca acagtcaggt gagagatgct gcaatattgg ctatagtgga gatttataga
      841 catgtgggag aaaaagtgag gatggatctt tataagagag gaattccccc tgctagatta
      901 gaaatgatat ttgccaaatt tgatgaagtg caaagttcag gcggtatgat tttgagtgtc
      961 tgcaaagata aaagcttcga tgatgaagaa tcagtggatg gaaataggcc atcatcagct
     1021 gcatcagcct tcaaggttcc tgcacctaaa acatccggaa atcctgccaa cagtgcaagg
     1081 aagcctggtt cagcaggtgg ccctaaggtt ggaggtgctt ctaaggaagg aggtgctgga
     1141 gcagttgatg aagatgattt tataaaagct tttacagatg tcccttctat tcagatttat
     1201 tctagtcgag aactcgaaga aacattaaat aaaatcaggg aaattttgtc agatgataaa
     1261 catgactggg atcagcgtgc caatgcactg aagaaaattc gatcactgct tgttgctgga
     1321 gctgcacagt atgattgctt ttttcaacat ttacgattgt tggatggagc acttaaactt
     1381 tcagctaagg atcttagatc ccaggtggtt agagaagctt gtattactgt agcccacctt
     1441 tcaacagttt tgggaaacaa gtttgatcat ggcgctgaag ccattgtacc tacacttttt
     1501 aatctcgtcc ccaatagtgc aaaagtcatg gcaacttctg gatgtgcagc aatcagattt
     1561 atcattcggc atactcatgt acccagactt atacctttaa taacaagcaa ttgcacatca
     1621 aaatcagttc ccgtgaggag acgttcattt gaatttttag atttattgtt gcaagagtgg
     1681 cagactcatt cattggaaag acatgcagcc gtcttggttg aaactattaa aaagggaatt
     1741 catgatgctg acgctgaggc cagagtggag gcaagaaaga catacatggg tcttagaaac
     1801 cactttcctg gtgaagctga aacattatat aattcccttg agccatctta tcagaagagt
     1861 cttcaaactt acttaaagag ttctggcagt gtagcatctc ttccacaatc agacaggtcc
     1921 tcatccagct cacaggaaag tctcaatcgc cctttttctt ccaaatggtc tacagcaaat
     1981 ccatcaactg tggctggaag agtatcagca ggcagcagca aagccagttc ccttccagga
     2041 agcctgcagc gttcacgaag tgacattgat gtgaatgctg ctgcaggtgc caaggcacat
     2101 catgctgctg gacagtctgt gcgaagcggg cgcttaggtg caggtgccct gaatgcaggt
     2161 tcctatgcgt cactagagga tacttctgac aagctggatg gaacagcatc tgaagatggc
     2221 cgggtgagag caaaactttc agcaccactt gctggcatgg gaaatgccaa ggcagattct
     2281 agaggaagaa gtcgaacaaa aatggtgtct caatcacagc ctggtagccg gtctgggtct
     2341 ccaggaagag ttctgaccac aacagccctg tccactgtga gctctggtgt tcaaagagtc
     2401 ctggtcaatt cagcctcagc acaaaaaaga agcaagatac cacggagcca gggctgtagc
     2461 agagaggcta gtccatctag gctttcagtg gcccgaagca gtcgtattcc tcgaccaagt
     2521 gtgagtcaag gatgcagccg ggaagctagt cgggagagca gcagagacac aagtcctgtt
     2581 cgctcttttc agcccctcgc ctccagacac cattccagat caactggtgc cctctacgcc
     2641 cccgaagtgt atggggcctc aggtccaggt tatgggatca gccaatcaag tcgactgtcg
     2701 tcttctgtta gtgccatgcg agtcctgaac acaggttctg atgtggagga ggcggtggca
     2761 gatgccttgc tcttaggaga catacggact aagaaaaaac cagctcgaag aagatatgaa
     2821 tcatatggaa tgcattcaga tgatgacgcc aacagcgatg catctagtgc ttgttcagaa
     2881 cgctcctata gttctcgaaa tggtagtatt cctacatata tgaggcagac ggaagatgtg
     2941 gcagaagtcc tcaatagatg tgctagttcc aattggtcag aaaggaaaga aggcctccta
     3001 ggtctgcaga acttattaaa aaatcagaga acactaagtc gagttgaact gaaaagatta
     3061 tgtgaaattt tcacaagaat gtttgctgac cctcatggca aggtattcag catgtttttg
     3121 gagactctag tggatttcat acaagtccac aaagatgatc ttcaagattg gttgtttgta
     3181 ctgctgacac aactactaaa aaaaatgggt gctgatttgc ttggatctgt tcaggcaaaa
     3241 gttcagaaag cccttgatgt tacaagagag tcttttccaa atgatcttca gttcaatatt
     3301 ctaatgagat ttacagttga tcagacccag acaccaagct taaaggtgaa ggttgctatc
     3361 cttaaataca tagaaactct ggccaaacag atggatccag gagattttat aaattccagt
     3421 gaaactcgcc tagcagtgtc tcgggtcatc acttggacaa cagaacccaa aagttctgat
     3481 gttcggaagg cagcacagtc agtgctgatt tcattatttg aactcaatac cccagagttt
     3541 acaatgttat taggagcttt accaaaaact tttcaggatg gtgctaccaa gcttcttcat
     3601 aatcaccttc gaaacactgg caatggaacc cagagttcca tggggagtcc tttgacaaga
     3661 ccaacaccac gatcaccagc taactggtcc agtcctctta cttctcctac caatacatca
     3721 cagaatactt tatctccaag tgcatttgat tatgacacag aaaatatgaa ctctgaagat
     3781 atttatagct ctcttagagg tgtcactgaa gcaatccaga atttcagctt ccgtagccaa
     3841 gaagatatga atgagccatt gaaaagggat tctaaaaaag atgatggcga ttcaatgtgt
     3901 ggtggtcctg ggatgtctga cccaagagca ggaggtgatg ctactgactc aagtcaaaca
     3961 gctcttgata ataaagcttc attgctccat tcaatgccta ctcactcctc tccacgctct
     4021 cgagactata atccatataa ctattcagat agcatcagtc ccttcaacaa gtctgccctc
     4081 aaggaagcca tgtttgatga tgatgctgac cagtttcctg acgatctttc cctagatcat
     4141 tctgacctag ttgcagagtt gttgaaggag ctgtctaacc ataatgagcg tgtagaagaa
     4201 agaaaaattg ccctctatga acttatgaaa ctgacacagg aagaatcttt tagtgtttgg
     4261 gatgaacact tcaaaacaat attgctttta ttgcttgaaa cgcttggaga taaagagcct
     4321 acaatcaggg ctttggcatt aaaggtttta agagaaatcc taaggcatca accagcaaga
     4381 tttaaaaact atgcagaatt gactgtcatg aaaacattgg aagcacataa agatcctcat
     4441 aaggaggtgg tgagatctgc tgaggaagcg gcatcagtgt tggccacttc aattagtcca
     4501 gagcagtgca tcaaagtgct ttgtcctatc attcaaactg cagactaccc aattaatctg
     4561 gctgcaatca aaatgcaaac aaaagtgata gagagagtgt ccaaggaaac cctaaacctg
     4621 cttttgccag agattatgcc aggtctaata cagggttatg ataattcaga gagcagtgtt
     4681 cggaaagctt gtgtcttctg cctggtggct gttcatgcgg taattggtga tgaactaaaa
     4741 ccacatctca gtcaacttac tggcagtaaa atgaagctac tgaatcttta catcaaacgt
     4801 gcacaaacag gttctggagg agctgatccc actactgatg tttctggaca aagttagtga
     4861 agctcatcac agcgaaccag gtctctcaaa agaaaggaca gatagaccac cctcatcaat
     4921 gaaaggaagt tctcaaacac atcctttgga acttactatt gtttcccagt tttagttttt
     4981 tgtttcgttt cgttttgtat tttctgtaac agaggactat cctcagtctg catgtaactt
     5041 ttatgatagt tattccaaat tcaagaagaa gcagtattaa catcaattga tcgacacaaa
     5101 gtaattttta atttaattca tcatttcaca tgtttgtact ttgtcttccc attaaccttt
     5161 gccagtgtta tgattgtata aattttttta aatgctggtt aaacaggaat gcttaaagct
     5221 ttaaaagttt aacagtctaa aacatttttg cttttattca actgcagaat aatattttta
     5281 ttgctacttt gagttttgtt tcgtatcatg tcctatgcta gaaatattta aatgatgtga
     5341 aacaaagcag gactaatttg aactacagct ggactccgtt tgtgtgatgg tgatacatgt
     5401 cattagttgc aacttctttg gggtgatcta tagtttgaaa actaaaacct caaagacaga
     5461 tgttacagaa tcagccagtt ctgtaaaact gatattgtct attggttatt gatcttgcca
     5521 tctttattta aaaccatgtc ccttctatga tcccttaaga aagctgcacc aaatcatctg
     5581 cctgtttttt cttgatactt actgaaatag aaggttttat tgcagggttt attttggttt
     5641 gtttatatct ttgttgtgaa tgatgctttt ttgtatttat taatatcaaa ttcacttatg
     5701 aataaacttg ataatggaaa cggacaaaaa aaatcaagtg cgtgtgtgtc cttgaccgtc
     5761 ttctgtttct cacgtaataa acaaattatc gagacatggg agtgaccagc accttttctt
     5821 taaatggtgg aacctggttt ccttttacca tgaaattgtc ttacttgaaa atattgatcc
     5881 tgatgagaga gaagatggtg ccaaggctgt ctttgtataa tgggctcaaa ttctctacct
     5941 cttcagggct aatactttta actgagctgc tgcctatagt gtcttttgga aaactactta
     6001 aagggtgatt ttctgttact ttttagcaaa tttttttaat cacctcttgc tacacccatt
     6061 cttttcatgt gcagccgact caaaaattac cagttttggt gaaaggctaa attagataat
     6121 ttggaaccag gatactaatg atttctcatc tttacttttt tttaatccta atataaagtg
     6181 aatttgattg aaaaggcaaa tagctattag ggaagcagtt tgccattgtt gcagagttat
     6241 ctgtactttg tttaactgaa aaaaatgtag aaatatatgt aaagaattta agacaagagt
     6301 actgaatgga tgatttgtca taggctttcc cctttctttc tgttctagca gcaggaaaag
     6361 tttctctata tcctctccct ctacctgtaa caattttgtt ttctactgtt aattacattg
     6421 tgtatttata gttctatgct tactgttgtg catatactgg caataaaact gtacataaca
     6481 ttacttgaaa aagttaataa tgtatatcag tttttctgtc tcactgtgta acaagtcact
     6541 cagttttatt ttaactttag acggtcttgt atcagtggtg gtctcttgaa ttttgtaagt
     6601 tcatctgagg agaaaagatt tttcaggtgt agctaccaca atcaaaggta tatagctaca
     6661 tacgcatgta tatattacag cttatctgta agaagaaaat gcattttaaa cacaactctt
     6721 ctcagtagca ttttatgacc tttggatatg tttgtaatca tttcgaatca aaatattgat
     6781 ttaattttga cctctggttt aagatactgc tttaactact gttgacaacc aagtagagtg
     6841 acttaagctg aacagtaact aactggaaaa ttagataagc acctggcatc taatggcagg
     6901 caggcactca agaaatgaat taactacata atggaaaagt atggtttaat gtgtccaaat
     6961 gaaagctagt agatgtaaac atggaaaaat tgtgtttaca attttataat ctcagttgat
     7021 aagactataa gaaagctgat tatttaaatc actatataca atacaccctt aatttgttca
     7081 ttccagaaac atactgagat gtcagctact taaaaatggt cacaaaaagc tactgtttat
     7141 atttttcctc ctgctattct ctcccaaatt aattattaat aagtgttgtt catttactgc
     7201 actgctgaga actaattaaa attatatatt ccagattgta aaa
//
