LOCUS       NM_001395891            8141 bp    mRNA    linear   PRI 22-SEP-2024
DEFINITION  Homo sapiens cytoplasmic linker associated protein 1 (CLASP1),
            transcript variant 8, mRNA.
ACCESSION   NM_001395891
VERSION     NM_001395891.1
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8141)
  AUTHORS   Segal,D., Maier,S., Mastromarco,G.J., Qian,W.W., Nabeel-Shah,S.,
            Lee,H., Moore,G., Lacoste,J., Larsen,B., Lin,Z.Y.,
            Selvabaskaran,A., Liu,K., Smibert,C., Zhang,Z., Greenblatt,J.,
            Peng,J., Lee,H.O., Gingras,A.C. and Taipale,M.
  TITLE     A central chaperone-like role for 14-3-3 proteins in human cells
  JOURNAL   Mol Cell 83 (6), 974-993 (2023)
   PUBMED   36931259
REFERENCE   2  (bases 1 to 8141)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   3  (bases 1 to 8141)
  AUTHORS   Mani,N., Jiang,S., Neary,A.E., Wijeratne,S.S. and Subramanian,R.
  TITLE     Differential regulation of single microtubules and bundles by a
            three-protein module
  JOURNAL   Nat Chem Biol 17 (9), 964-974 (2021)
   PUBMED   34083810
  REMARK    GeneRIF: Differential regulation of single microtubules and bundles
            by a three-protein module.
REFERENCE   4  (bases 1 to 8141)
  AUTHORS   Ferreira,L.T., Logarinho,E., Macedo,J.C., Maia,A.R.R. and Maiato,H.
  TITLE     SOGA1 and SOGA2/MTCL1 are CLASP-interacting proteins required for
            faithful chromosome segregation in human cells
  JOURNAL   Chromosome Res 29 (2), 159-173 (2021)
   PUBMED   33587225
REFERENCE   5  (bases 1 to 8141)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   6  (bases 1 to 8141)
  AUTHORS   Takahashi,M., Yamagiwa,A., Nishimura,T., Mukai,H. and Ono,Y.
  TITLE     Centrosomal proteins CG-NAP and kendrin provide microtubule
            nucleation sites by anchoring gamma-tubulin ring complex
  JOURNAL   Mol Biol Cell 13 (9), 3235-3245 (2002)
   PUBMED   12221128
REFERENCE   7  (bases 1 to 8141)
  AUTHORS   Akhmanova,A., Hoogenraad,C.C., Drabek,K., Stepanova,T.,
            Dortland,B., Verkerk,T., Vermeulen,W., Burgering,B.M., De
            Zeeuw,C.I., Grosveld,F. and Galjart,N.
  TITLE     Clasps are CLIP-115 and -170 associating proteins involved in the
            regional regulation of microtubule dynamics in motile fibroblasts
  JOURNAL   Cell 104 (6), 923-935 (2001)
   PUBMED   11290329
REFERENCE   8  (bases 1 to 8141)
  AUTHORS   Mayor,T., Stierhof,Y.D., Tanaka,K., Fry,A.M. and Nigg,E.A.
  TITLE     The centrosomal protein C-Nap1 is required for cell cycle-regulated
            centrosome cohesion
  JOURNAL   J Cell Biol 151 (4), 837-846 (2000)
   PUBMED   11076968
REFERENCE   9  (bases 1 to 8141)
  AUTHORS   Lemos,C.L., Sampaio,P., Maiato,H., Costa,M., Omel'yanchuk,L.V.,
            Liberal,V. and Sunkel,C.E.
  TITLE     Mast, a conserved microtubule-associated protein required for
            bipolar mitotic spindle organization
  JOURNAL   EMBO J 19 (14), 3668-3682 (2000)
   PUBMED   10899121
REFERENCE   10 (bases 1 to 8141)
  AUTHORS   Golsteyn,R.M., Mundt,K.E., Fry,A.M. and Nigg,E.A.
  TITLE     Cell cycle regulation of the activity and subcellular localization
            of Plk1, a human protein kinase implicated in mitotic spindle
            function
  JOURNAL   J Cell Biol 129 (6), 1617-1628 (1995)
   PUBMED   7790358
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC018737.9, AC079449.5, AC012447.8 and AC013399.6.
            
            Summary: CLASPs, such as CLASP1, are nonmotor
            microtubule-associated proteins that interact with CLIPs (e.g.,
            CLIP170; MIM 179838). CLASP1 is involved in the regulation of
            microtubule dynamics at the kinetochore and throughout the spindle
            (Maiato et al., 2003 [PubMed 12837247]).[supplied by OMIM, Mar
            2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: mixed sample support SAMEA1965299, SAMEA1966682
                              [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            inferred exon combination :: based on alignments, homology
            MANE Ensembl match        :: ENST00000696935.1/ ENSP00000512981.1
            RefSeq Select criteria    :: based on manual assertion,
                                         conservation
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-91                AC018737.9         43473-43563         c
            92-372              AC018737.9         1-281               c
            373-571             AC079449.5         41513-41711         c
            572-650             AC012447.8         130364-130442       c
            651-754             AC012447.8         128794-128897       c
            755-846             AC012447.8         127916-128007       c
            847-922             AC012447.8         125962-126037       c
            923-1020            AC012447.8         115782-115879       c
            1021-1088           AC012447.8         103284-103351       c
            1089-1241           AC012447.8         69925-70077         c
            1242-1315           AC012447.8         62649-62722         c
            1316-1408           AC012447.8         61218-61310         c
            1409-1554           AC012447.8         60097-60242         c
            1555-1690           AC012447.8         58957-59092         c
            1691-1761           AC012447.8         57804-57874         c
            1762-1821           AC012447.8         51907-51966         c
            1822-1899           AC012447.8         51030-51107         c
            1900-2067           AC012447.8         49070-49237         c
            2068-2117           AC012447.8         48393-48442         c
            2118-2288           AC012447.8         47454-47624         c
            2289-2393           AC012447.8         30190-30294         c
            2394-2420           AC012447.8         27521-27547         c
            2421-2588           AC012447.8         25256-25423         c
            2589-2696           AC012447.8         18739-18846         c
            2697-2759           AC012447.8         14258-14320         c
            2760-2863           AC012447.8         10983-11086         c
            2864-3108           AC012447.8         7588-7832           c
            3109-3172           AC012447.8         4488-4551           c
            3173-3175           AC012447.8         3894-3896           c
            3176-3339           AC012447.8         1626-1789           c
            3340-3418           AC013399.6         168251-168329       c
            3419-3562           AC013399.6         167069-167212       c
            3563-3706           AC013399.6         157692-157835       c
            3707-3813           AC013399.6         157051-157157       c
            3814-3930           AC013399.6         152137-152253       c
            3931-4081           AC013399.6         147428-147578       c
            4082-4325           AC013399.6         137517-137760       c
            4326-4516           AC013399.6         135023-135213       c
            4517-4645           AC013399.6         133101-133229       c
            4646-4852           AC013399.6         118441-118647       c
            4853-4969           AC013399.6         116967-117083       c
            4970-8141           AC013399.6         107705-110876       c
FEATURES             Location/Qualifiers
     source          1..8141
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q14.2-q14.3"
     gene            1..8141
                     /gene="CLASP1"
                     /gene_synonym="MAST1"
                     /note="cytoplasmic linker associated protein 1"
                     /db_xref="GeneID:23332"
                     /db_xref="HGNC:HGNC:17088"
                     /db_xref="MIM:605852"
     exon            1..91
                     /gene="CLASP1"
                     /gene_synonym="MAST1"
                     /inference="alignment:Splign:2.1.0"
     exon            92..571
                     /gene="CLASP1"
                     /gene_synonym="MAST1"
                     /inference="alignment:Splign:2.1.0"
     CDS             377..5056
                     /gene="CLASP1"
                     /gene_synonym="MAST1"
                     /note="isoform 8 is encoded by transcript variant 8;
                     CLIP-associating protein 1; multiple asters 1; protein
                     Orbit homolog 1; multiple asters homolog 1"
                     /codon_start=1
                     /product="CLIP-associating protein 1 isoform 8"
                     /protein_id="NP_001382820.1"
                     /db_xref="CCDS:CCDS92853.1"
                     /db_xref="GeneID:23332"
                     /db_xref="HGNC:HGNC:17088"
                     /db_xref="MIM:605852"
                     /translation="MEPRMESCLAQVLQKDVGKRLQVGQELIDYFSDKQKSADLEHDQ
                     TMLDKLVDGLATSWVNSSNYKVVLLGMDILSALVTRLQDRFKAQIGTVLPSLIDRLGD
                     AKDSVREQDQTLLLKIMDQAANPQYVWDRMLGGFKHKNFRTREGICLCLIATLNASGA
                     QTLTLSKIVPHICNLLGDPNSQVRDAAINSLVEIYRHVGERVRADLSKKGLPQSRLNV
                     IFTKFDEVQKSGNMIQSANDKNFDDEDSVDGNRPSSASSTSSKAPPSSRRNVGMGTTR
                     RLGSSTLGSKSSAAKEGAGAVDEEDFIKAFDDVPVVQIYSSRDLEESINKIREILSDD
                     KHDWEQRVNALKKIRSLLLAGAAEYDNFFQHLRLLDGAFKLSAKDLRSQVVREACITL
                     GHLSSVLGNKFDHGAEAIMPTIFNLIPNSAKIMATSGVVAVRLIIRHTHIPRLIPVIT
                     SNCTSKSVAVRRRCFEFLDLLLQEWQTHSLERHISVLAETIKKGIHDADSEARIEARK
                     CYWGFHSHFSREAEHLYHTLESSYQKALQSHLKNSDSIVSLPQSDRSSSSSQESLNRP
                     LSAKRSPTGSTTSRASTVSTKSVSTTGSLQRSRSDIDVNAAASAKSKVSSSSGTTPFS
                     SAAALPPGSYASLGRIRTRRQSSGSATNVASTPDNRGRSRAKVVSQSQRSRSANPAGA
                     GSRSSSPGKLLGSGYGGLTGGSSRGPPVTPSSEKRSKIPRSQGCSRETSPNRIGLARS
                     SRIPRPSMSQGCSRDTSRESSRDTSPARGFPPLASRRHSRSTSALSTAESVGQSDRFG
                     LGQPGRIPGSVNAMRVLSTSTDLEAAVADALKKPVRRRYEPYGMYSDDDANSDASSVC
                     SERSYGSRNGGIPHYLRQTEDVAEVLNHCASSNWSERKEGLLGLQNLLKSQRTLSRVE
                     LKRLCEIFTRMFADPHSKRVFSMFLETLVDFIIIHKDDLQDWLFVLLTQLLKKMGADL
                     LGSVQAKVQKALDVTRDSFPFDQQFNILMRFIVDQTQTPNLKVKVAILKYIESLARQM
                     DPTDFVNSSETRLAVSRIITWTTEPKSSDVRKAAQIVLISLFELNTPEFTMLLGALPK
                     TFQDGATKLLHNHLKNSSNTSVGSPSNTIGRTPSRHTSSRTSPLTSPTNCSHGGLSPS
                     RLWGWSADGLAKHPPPFSQPNSIPTAPSHKALRRSYSPSMLDYDTENLNSEEIYSSLR
                     GVTEAIEKFSFRSQEDLNEPIKRDGKKECDIVSRDGGAASPATEGRGGSEVEGGRTAL
                     DNKTSLLNTQPPRAFPGPRARDYNPYPYSDAINTYDKTALKEAVFDDDMEQLRDVPID
                     HSDLVADLLKELSNHNERVEERKGALLELLKITREDSLGVWEEHFKTILLLLLETLGD
                     KDHSIRALALRVLREILRNQPARFKNYAELTIMKTLEAHKDSHKEVVRAAEEAASTLA
                     SSIHPEQCIKVLCPIIQTADYPINLAAIKMQTKVVERIAKESLLQLLVDIIPGLLQGY
                     DNTESSVRKASVFCLVAIYSVIGEDLKPHLAQLTGSKMKLLNLYIKRAQTTNSNSSSS
                     SDVSTHS"
     exon            572..650
                     /gene="CLASP1"
                     /gene_synonym="MAST1"
                     /inference="alignment:Splign:2.1.0"
     exon            651..754
                     /gene="CLASP1"
                     /gene_synonym="MAST1"
                     /inference="alignment:Splign:2.1.0"
     exon            755..846
                     /gene="CLASP1"
                     /gene_synonym="MAST1"
                     /inference="alignment:Splign:2.1.0"
     exon            847..922
                     /gene="CLASP1"
                     /gene_synonym="MAST1"
                     /inference="alignment:Splign:2.1.0"
     exon            923..1020
                     /gene="CLASP1"
                     /gene_synonym="MAST1"
                     /inference="alignment:Splign:2.1.0"
     exon            1021..1088
                     /gene="CLASP1"
                     /gene_synonym="MAST1"
                     /inference="alignment:Splign:2.1.0"
     exon            1089..1241
                     /gene="CLASP1"
                     /gene_synonym="MAST1"
                     /inference="alignment:Splign:2.1.0"
     exon            1242..1315
                     /gene="CLASP1"
                     /gene_synonym="MAST1"
                     /inference="alignment:Splign:2.1.0"
     exon            1316..1408
                     /gene="CLASP1"
                     /gene_synonym="MAST1"
                     /inference="alignment:Splign:2.1.0"
     exon            1409..1554
                     /gene="CLASP1"
                     /gene_synonym="MAST1"
                     /inference="alignment:Splign:2.1.0"
     exon            1555..1690
                     /gene="CLASP1"
                     /gene_synonym="MAST1"
                     /inference="alignment:Splign:2.1.0"
     exon            1691..1761
                     /gene="CLASP1"
                     /gene_synonym="MAST1"
                     /inference="alignment:Splign:2.1.0"
     exon            1762..1821
                     /gene="CLASP1"
                     /gene_synonym="MAST1"
                     /inference="alignment:Splign:2.1.0"
     exon            1822..1899
                     /gene="CLASP1"
                     /gene_synonym="MAST1"
                     /inference="alignment:Splign:2.1.0"
     exon            1900..2067
                     /gene="CLASP1"
                     /gene_synonym="MAST1"
                     /inference="alignment:Splign:2.1.0"
     exon            2068..2117
                     /gene="CLASP1"
                     /gene_synonym="MAST1"
                     /inference="alignment:Splign:2.1.0"
     exon            2118..2288
                     /gene="CLASP1"
                     /gene_synonym="MAST1"
                     /inference="alignment:Splign:2.1.0"
     exon            2289..2393
                     /gene="CLASP1"
                     /gene_synonym="MAST1"
                     /inference="alignment:Splign:2.1.0"
     exon            2394..2420
                     /gene="CLASP1"
                     /gene_synonym="MAST1"
                     /inference="alignment:Splign:2.1.0"
     exon            2421..2588
                     /gene="CLASP1"
                     /gene_synonym="MAST1"
                     /inference="alignment:Splign:2.1.0"
     exon            2589..2696
                     /gene="CLASP1"
                     /gene_synonym="MAST1"
                     /inference="alignment:Splign:2.1.0"
     exon            2697..2759
                     /gene="CLASP1"
                     /gene_synonym="MAST1"
                     /inference="alignment:Splign:2.1.0"
     exon            2760..2863
                     /gene="CLASP1"
                     /gene_synonym="MAST1"
                     /inference="alignment:Splign:2.1.0"
     exon            2864..3108
                     /gene="CLASP1"
                     /gene_synonym="MAST1"
                     /inference="alignment:Splign:2.1.0"
     exon            3109..3172
                     /gene="CLASP1"
                     /gene_synonym="MAST1"
                     /inference="alignment:Splign:2.1.0"
     exon            3173..3175
                     /gene="CLASP1"
                     /gene_synonym="MAST1"
                     /inference="alignment:Splign:2.1.0"
     exon            3176..3339
                     /gene="CLASP1"
                     /gene_synonym="MAST1"
                     /inference="alignment:Splign:2.1.0"
     exon            3340..3418
                     /gene="CLASP1"
                     /gene_synonym="MAST1"
                     /inference="alignment:Splign:2.1.0"
     exon            3419..3562
                     /gene="CLASP1"
                     /gene_synonym="MAST1"
                     /inference="alignment:Splign:2.1.0"
     exon            3563..3706
                     /gene="CLASP1"
                     /gene_synonym="MAST1"
                     /inference="alignment:Splign:2.1.0"
     exon            3707..3813
                     /gene="CLASP1"
                     /gene_synonym="MAST1"
                     /inference="alignment:Splign:2.1.0"
     exon            3814..3930
                     /gene="CLASP1"
                     /gene_synonym="MAST1"
                     /inference="alignment:Splign:2.1.0"
     exon            3931..4081
                     /gene="CLASP1"
                     /gene_synonym="MAST1"
                     /inference="alignment:Splign:2.1.0"
     exon            4082..4325
                     /gene="CLASP1"
                     /gene_synonym="MAST1"
                     /inference="alignment:Splign:2.1.0"
     exon            4326..4516
                     /gene="CLASP1"
                     /gene_synonym="MAST1"
                     /inference="alignment:Splign:2.1.0"
     exon            4517..4645
                     /gene="CLASP1"
                     /gene_synonym="MAST1"
                     /inference="alignment:Splign:2.1.0"
     exon            4646..4852
                     /gene="CLASP1"
                     /gene_synonym="MAST1"
                     /inference="alignment:Splign:2.1.0"
     exon            4853..4969
                     /gene="CLASP1"
                     /gene_synonym="MAST1"
                     /inference="alignment:Splign:2.1.0"
     exon            4970..8141
                     /gene="CLASP1"
                     /gene_synonym="MAST1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7844..7849
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CLASP1"
                     /gene_synonym="MAST1"
                     /note="hexamer: AATAAA"
     polyA_site      7866
                     /gene="CLASP1"
                     /gene_synonym="MAST1"
                     /note="major polyA site"
     regulatory      8121..8126
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CLASP1"
                     /gene_synonym="MAST1"
                     /note="hexamer: AATAAA"
     polyA_site      8141
                     /gene="CLASP1"
                     /gene_synonym="MAST1"
                     /note="major polyA site"
ORIGIN      
        1 atccccgcac caagcgctta acctcattgg ggtggaggag aaggcggcgg ctctctggtc
       61 cgcagcggca acagtaacga aaaacagggc taatggactg ctgaattatg aagtatttca
      121 gacccagtag tagaacatca ctctgccact cactccttta tctcctacta gttatttaaa
      181 ttggactttt aatatcctac cagctgctct tcagacacac atggtgcatt gttgccattc
      241 cccagattgc atctttgaaa cacaggctct tagtaacctt cagcgaacaa agaggcaacc
      301 tcccagatac gtctgctggg agggagtcat cgtgactgcc ctctagcttt tgctggatct
      361 ggatttgaat tccactatgg agcctcgcat ggagtcctgc ctggcgcagg tgttgcagaa
      421 ggatgtgggg aaacgattgc aggttggcca agaactgata gactatttct cagacaaaca
      481 gaagtctgct gaccttgagc atgaccagac catgttagat aaacttgtgg atggacttgc
      541 tacctcttgg gtgaactcta gcaattacaa ggtggttctg ctgggcatgg acatcctgtc
      601 cgccctggtg acccggctgc aggatcggtt caaggcgcag atcggcacag tgctgccaag
      661 tctaatagac agactaggag atgctaaaga ctctgtgagg gagcaggacc aaactctgct
      721 gctaaagatc atggatcaag ctgctaatcc ccagtacgta tgggacagaa tgcttggagg
      781 cttcaaacac aagaatttcc gtactcgaga aggcatctgt ctctgcctta tagcaacact
      841 caatgcctct ggagcacaga ctttaacact aagcaagatt gtgccacata tatgcaactt
      901 acttggagat ccaaacagcc aggttcgaga tgcagcaata aacagcttag tggaaattta
      961 cagacatgta ggagaacgtg tgagggcaga tctcagtaaa aaaggattgc cacagtcccg
     1021 gttgaatgta atttttacaa aatttgatga agtccagaaa tctggaaaca tgatacaatc
     1081 tgcaaatgat aaaaatttcg acgatgaaga ttctgtggat ggtaacagac cttcctctgc
     1141 tagttctaca tcatccaagg ctccaccaag ttctcggaga aacgttggaa tgggaaccac
     1201 ccgccggctt ggttcatcca cccttggatc caagtcttca gctgcaaaag aaggagctgg
     1261 tgctgttgat gaagaggatt ttattaaagc atttgatgat gtacctgtag tacagattta
     1321 ttccagccga gaccttgagg aatccataaa caaaattagg gaaatattat ctgatgacaa
     1381 gcatgattgg gagcagagag taaatgctct aaaaaagatt agatctttac ttttggctgg
     1441 tgctgctgag tatgataact tctttcaaca tttgcgtctt ttggatggag cctttaaact
     1501 ctctgctaag gacctgcggt ctcaggtagt gcgggaggct tgtatcacgt tggggcatct
     1561 gtcatcagtt ctggggaata agtttgacca tggagctgaa gccattatgc caactatctt
     1621 taatttaatt ccaaacagtg ccaaaattat ggccacatct ggtgttgtag ctgttaggtt
     1681 aattattcgg cacacacaca tccctaggtt aatacctgtc ataacaagca actgtacctc
     1741 taagtctgtc gcagttagaa ggcgctgttt tgaattttta gatttgcttt tacaagaatg
     1801 gcagacacat tcactagaac gacacatatc agtattagct gaaacaataa agaagggaat
     1861 acatgatgct gattccgaag caagaataga agccagaaaa tgttactggg gtttccacag
     1921 tcacttcagc agagaagcag agcacttgta ccacaccttg gagtcctcct accagaaagc
     1981 cctgcagtcc cacctgaaga actcagacag catagtgtct ctgcctcagt cagaccgctc
     2041 atcttccagc tctcaagaga gtctaaatcg tccgctgtct gccaaaagaa gtcctactgg
     2101 aagtaccaca tctagagctt ctacagttag taccaaatct gtgtcaacga ctgggtccct
     2161 ccagcgatct cgaagtgata ttgatgtgaa cgcagcagcc agtgccaaat ccaaagtctc
     2221 ctcatcttcg ggcacgacgc ctttcagctc tgcagcagct ttgcctccag ggtcatacgc
     2281 atccttaggt cggatccgca caagacggca aagctctggg agtgccacca acgtcgcctc
     2341 tacacctgat aaccggggcc gcagtcgcgc taaagtggtt tcacagtccc agcgatccag
     2401 atctgctaat cctgctggtg ctggcagccg gtcaagttcc ccaggaaaat tgttgggaag
     2461 tggttatggt ggacttactg ggggctcctc acgaggccca cctgtgacac cgtcttcaga
     2521 aaagcgaagc aagattccca ggagccaggg atgtagccgg gaaacaagtc caaaccgaat
     2581 aggattagca cggagcagcc gtatccctcg acccagcatg agtcaggggt gcagccgcga
     2641 taccagccgt gagagcagcc gagatacaag ccctgctcgg ggctttcctc cacttgcctc
     2701 tcgacgtcat tccaggtcca ctagtgctct ctccactgct gaatctgttg ggcagtcaga
     2761 tcggtttggg cttggccagc caggaagaat acctggttct gtgaatgcca tgagagttct
     2821 gagcacaagt acagatcttg aagctgctgt tgctgatgct ttgaagaagc ctgtgaggag
     2881 gagatatgag ccgtatggga tgtattctga cgatgatgcc aacagtgatg cctcaagtgt
     2941 ttgctctgag cgctcatatg gctccaggaa tggtggcatt ccccattatc tgcggcagac
     3001 tgaggatgta gcagaagttc tcaaccactg tgctagttca aactggtcag aaaggaaaga
     3061 agggcttctg ggcctgcaga acttactgaa gagccaaaga acactgagtc gagttgaact
     3121 gaaaaggttg tgtgagatct tcactcggat gtttgctgac cctcatagca agagagtttt
     3181 cagtatgttt ttggagactc ttgtggattt tataataatt cataaggatg atttacaaga
     3241 ctggcttttt gttcttctca cacaattact taagaaaatg ggagcagatt tacttggatc
     3301 tgtgcaagca aaagttcaaa aggctctaga tgtcacaagg gactcctttc catttgatca
     3361 acaatttaac attttgatga gatttattgt ggatcaaact caaactccaa acctcaaggt
     3421 caaagttgca atcctgaaat acattgagtc tctggccaga cagatggatc caacagattt
     3481 tgtaaactct agtgagacaa ggcttgctgt ttctagaatc ataacctgga caacagaacc
     3541 aaagagttca gacgtgagaa aggcagcaca gattgtgcta atctctctgt ttgaattgaa
     3601 tactcctgaa tttaccatgt tacttggtgc cttgccaaaa acattccagg atggtgccac
     3661 caaactcctg cacaaccacc tcaagaattc cagtaacacc agtgtgggct ctccaagcaa
     3721 tacgattggc cggacgccct cccgacacac cagcagcagg accagccccc tgacctcacc
     3781 caccaactgt tcccatgggg gtctgtctcc aagtcggtta tggggttgga gtgccgacgg
     3841 gttagcgaag cacccacctc ccttttctca gcctaactcc atccccaccg ctccctccca
     3901 caaggctctc aggcgctctt actctcccag catgctggac tatgatacag agaacctgaa
     3961 ctctgaagaa atctatagtt ctctacgtgg agttacagaa gccattgaaa agtttagttt
     4021 tcgaagccaa gaagatctga atgagccaat taaacgagat ggcaaaaagg agtgtgatat
     4081 tgtgtcccgc gatgggggcg ctgcctcccc tgccactgag ggccgggggg gtagtgaagt
     4141 agaaggaggc cggacagctc tggataacaa gacctcacta ctcaacaccc agcctccgcg
     4201 cgccttcccg gggccgcggg cgcgagacta caacccgtac ccctactcag atgccatcaa
     4261 cacctacgac aagaccgccc tgaaagaggc tgtgttcgat gacgacatgg agcagcttcg
     4321 agacgtgccc atcgaccatt ctgacctggt ggctgacctt ctgaaagagc tgtccaacca
     4381 caatgagcga gtggaggaac ggaagggagc cctgctggag ctgctcaaga tcacgcggga
     4441 agacagcctt ggtgtctggg aggagcactt caagaccatt ctgctcctgc tgctggagac
     4501 ccttggagac aaagaccatt caattcgagc actggcgtta agagttttga gggaaattct
     4561 gagaaatcaa ccagcaagat ttaaaaacta cgccgagctg acgattatga agactctgga
     4621 agcccacaaa gactcccata aggaggtggt gagagcggct gaggaggctg cgtccacact
     4681 ggccagttcc atccacccgg agcagtgcat caaggtgctc tgccccatca tccagacggc
     4741 cgactacccc atcaaccttg ctgccatcaa gatgcagacc aaagtcgtcg agaggatcgc
     4801 aaaggagtca ttgctgcagc tccttgtcga catcatccca ggcttgctgc agggttatga
     4861 caacaccgaa agtagtgtgc gtaaggccag cgtgttttgc ttagtggcaa tttattccgt
     4921 aatcggagaa gacctgaaac ctcaccttgc acagctcaca gggagcaaga tgaagctact
     4981 aaacttatac ataaagaggg cccagaccac caacagcaac agcagctcct cctccgatgt
     5041 ctccacgcac agctaatggc agtacctgtc tcttgtgtag acctagaagc aatcggtggt
     5101 gcctctcaga gacctttccc cacccccttc atcggctgcc cagtcagtac aaggaggccc
     5161 acaaatattt attacaatca gtattttggt cccttccagc ttttctgtag aatcttactg
     5221 gtattgaatg taaaggaagc aaggcctgta ttgcagtctt catacaaaac aaaaggaata
     5281 agaacagaaa agagccatac tgaaacatgt cttgtacagc ctgctgagat ggcgaaaccc
     5341 tgtgtgtggg gtgcagtttt taaaaatcag agcgctctag ccactacttg gtagaaagta
     5401 gcattttttt tttcagttaa taacatattt gggggtgggg tggggtgtta ctttgtgttc
     5461 ttcctcctta gcctattttc ttgtgcgtat ggtctgtgtg gggccccttt cacagctgac
     5521 accacgaaag gtgatatatc tttaagttgt gttctgagac ctactaaaaa tgggaatcaa
     5581 gtcttggcaa gaacagtctg aagatggcct tttaacaaac gctgggaatt ttgcttgtca
     5641 tatccagact ggaggccgac tgccctggct ttcagcgtag aattgggagt gcaccctgac
     5701 agtctccttc cagctctccc taatcgactc caccgacaag gtccctaccc cagagcttcc
     5761 atgcaaagga attcttcaag tttaaatctg gacacaaaaa taagataaat gtatggcatc
     5821 atttagggat gcctgagatg gcagttcatg aagcacagaa gataaagaag aagtctttca
     5881 tctttactgc tgagatcctt gggaacactg ttgtcatggg ggctctgcca agaccctcat
     5941 ctctgggcta cacggtgatt cagattgagc accaacttgt ttcctcccct caaagttctg
     6001 cctaagccgt tcagttctaa catggtctca gttaatctgg taaatggcat ctttaccatc
     6061 ttagttctga cttctcagtt taatgtggga ttaagagcca agaaaagcct agagagactg
     6121 gatatcacaa ttttttttaa ttttataaac tgaagtagtt ccttgaatgt ctgttgatga
     6181 aatagtcact gtttaaggaa aaaagtaatt atgaggtgta gcagattgca gaaaaacagg
     6241 attagaaaca cacttaaaaa gaacacacat ttagagtctc tcttcctcct cagcgaacca
     6301 ctaggccccc tttttaaaaa cacctttaga gcctaattac tccaataaaa gtaactagag
     6361 gtttggagtc tggttaaata aattctgagt aaaattctta agccaaatgg aaattcttaa
     6421 tgcaatcatg aggacttcta ttgtctctta ctgttgtatt agatcctata aattgaactg
     6481 atttttccat aaggaaaatg cttcttttga gattaattct aataacgtat ttgctattgc
     6541 agtgcagagc ccactgcaac tgctaggact gaaagcagag gctgggtgcc agagcacgtg
     6601 attcttaaca tcatttccac agacccctct gccctgaccc tctgcattgg atgcaggaag
     6661 ctgggaaaga ctgatgttga tttggaaaca tgggctgaaa atgaaggccc catagtgcat
     6721 aggaacagta aagccagggt gctgacgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt
     6781 gtgtggtgtt gtgtgtgttt gtgcgtgcac cctacacatg tgtggtacct cactgctgct
     6841 gtttagggaa cttgagggac gcgtttcaag gggttgggta ttactgacga gctttggctc
     6901 aaaatatagc aggaccaggt cttttgttga taagtactgt ttgtttatta atatgtcatt
     6961 aatggtattt cttttttaca ctctacaagt gaattaggga gtctcttgtt gacccctttg
     7021 ttgcaggaat gtgcgtcggg ctaggttatc catgagtttc tttattccta atgcagttag
     7081 aaagaccttt ctccttgagc tctttgactc ccagaaggta ccccagtccc cagtgtactt
     7141 agaaaggatc tcgaacattg ctggacgtcc tcatagtact cacaaagggc tagccttgaa
     7201 tgtcactcgc ccagtcttca gtctcctgac ttagagatac aatcacgtca caggtctctt
     7261 ggcctcaatc tgaaaactgc tgccgccgcg ccgaggagac tcgcatgccg ccaccacctc
     7321 actgggaggg cgccgagccc accgtcgccc cctagaccct gacagctgca gctgccttgc
     7381 cttgccgccg cctccctgca gggcccctgt tccaatgaaa aacagaacac aaaagagcag
     7441 agcacctaag cctgtctctg cctccctgtc taccggactg gccagggccc aagacccccg
     7501 ctgctccact gcggggctgg gcgggctgac tccctgcttc ctccaagctg ctgcctcccc
     7561 tgcagccagg gtctgggcag ggtgcagccg gtcctcgggg cacgcagctt ccttcaagta
     7621 cactgtgtgt gcttcccgga cctgcggcga tgccacgggc ctgccttttc tatgcgcctc
     7681 actagcttac caccctgtgc aggtaatgca actgactttg tctcatcagt ctttttcttt
     7741 ccctgccacc ctttatttat caagcgtaat gttacacttt aaaggacagc aaataagaac
     7801 tttgtagaat cccaccagga ctttgctaac aataatgttt ggaaataaag aagtgctctg
     7861 aaaaaatatc agccaccaaa atagttatgt tggcactgtg ttcacacgca tggtccccac
     7921 acccccaggt tgggtgggtt tttttgtttt ttgggttttt ttgggggggg gggctttttc
     7981 atgttacatc catatctgta tttatatctt atttgtttca ctttcaagtg tatcatggca
     8041 aatgtacaga tttttttgtt aataatgtgc taggatttgc taaaaaagaa aaaaaaaaaa
     8101 cccttttgag tttgccctag aataaatgag acttaattca a
//
