LOCUS       NP_001362413             417 aa            linear   PRI 29-APR-2025
DEFINITION  creatine kinase U-type, mitochondrial precursor [Homo sapiens].
ACCESSION   NP_001362413 XP_011519499
VERSION     NP_001362413.1
DBSOURCE    REFSEQ: accession NM_001375484.1
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (residues 1 to 417)
  AUTHORS   Ayyappan,V., Jenkinson,N.M., Tressler,C.M., Tan,Z., Cheng,M.,
            Shen,X.E., Guerrero,A., Sonkar,K., Cai,R., Adelaja,O., Roy,S.,
            Meeker,A., Argani,P. and Glunde,K.
  TITLE     Context-dependent roles for ubiquitous mitochondrial creatine
            kinase CKMT1 in breast cancer progression
  JOURNAL   Cell Rep 43 (4), 114121 (2024)
   PUBMED   38615320
  REMARK    GeneRIF: Context-dependent roles for ubiquitous mitochondrial
            creatine kinase CKMT1 in breast cancer progression.
REFERENCE   2  (residues 1 to 417)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   3  (residues 1 to 417)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (residues 1 to 417)
  AUTHORS   Shi,H., Song,Y., Song,Z. and Huang,C.
  TITLE     CKMT1B is a potential prognostic biomarker and associated with
            immune infiltration in Lower-grade glioma
  JOURNAL   PLoS One 16 (1), e0245524 (2021)
   PUBMED   33465115
  REMARK    GeneRIF: CKMT1B is a potential prognostic biomarker and associated
            with immune infiltration in Lower-grade glioma.
            Publication Status: Online-Only
REFERENCE   5  (residues 1 to 417)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   6  (residues 1 to 417)
  AUTHORS   Brdiczka,D., Kaldis,P. and Wallimann,T.
  TITLE     In vitro complex formation between the octamer of mitochondrial
            creatine kinase and porin
  JOURNAL   J Biol Chem 269 (44), 27640-27644 (1994)
   PUBMED   7525559
REFERENCE   7  (residues 1 to 417)
  AUTHORS   Haas,R.C. and Strauss,A.W.
  TITLE     Separate nuclear genes encode sarcomere-specific and ubiquitous
            human mitochondrial creatine kinase isoenzymes
  JOURNAL   J Biol Chem 265 (12), 6921-6927 (1990)
   PUBMED   2324105
REFERENCE   8  (residues 1 to 417)
  AUTHORS   Haas,R.C., Korenfeld,C., Zhang,Z.F., Perryman,B., Roman,D. and
            Strauss,A.W.
  TITLE     Isolation and characterization of the gene and cDNA encoding human
            mitochondrial creatine kinase
  JOURNAL   J Biol Chem 264 (5), 2890-2897 (1989)
   PUBMED   2914937
  REMARK    Erratum:[J Biol Chem 1989 Sep 25;264(27):16332]
REFERENCE   9  (residues 1 to 417)
  AUTHORS   Stallings,R.L., Olson,E., Strauss,A.W., Thompson,L.H.,
            Bachinski,L.L. and Siciliano,M.J.
  TITLE     Human creatine kinase genes on chromosomes 15 and 19, and proximity
            of the gene for the muscle form to the genes for apolipoprotein C2
            and excision repair
  JOURNAL   Am J Hum Genet 43 (2), 144-151 (1988)
   PUBMED   3400641
REFERENCE   10 (residues 1 to 417)
  AUTHORS   Jacobus,W.E., Moreadith,R.W. and Vandegaer,K.M.
  TITLE     Control of heart oxidative phosphorylation by creatine kinase in
            mitochondrial membranes
  JOURNAL   Ann N Y Acad Sci 414, 73-89 (1983)
   PUBMED   6584077
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC011330.8.
            
            On Oct 29, 2019 this sequence version replaced XP_011519499.1.
            
            Summary: Mitochondrial creatine (MtCK) kinase is responsible for
            the transfer of high energy phosphate from mitochondria to the
            cytosolic carrier, creatine. It belongs to the creatine kinase
            isoenzyme family. It exists as two isoenzymes, sarcomeric MtCK and
            ubiquitous MtCK, encoded by separate genes. Mitochondrial creatine
            kinase occurs in two different oligomeric forms: dimers and
            octamers, in contrast to the exclusively dimeric cytosolic creatine
            kinase isoenzymes. Many malignant cancers with poor prognosis have
            shown overexpression of ubiquitous mitochondrial creatine kinase;
            this may be related to high energy turnover and failure to
            eliminate cancer cells via apoptosis. Ubiquitous mitochondrial
            creatine kinase has 80% homology with the coding exons of
            sarcomeric mitochondrial creatine kinase. Two genes located near
            each other on chromosome 15 have been identified which encode
            identical mitochondrial creatine kinase proteins. [provided by
            RefSeq, Jul 2008].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC108652.1, SRR18074969.841260.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1966682, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            MANE Ensembl match                 :: ENST00000441322.6/
                                                  ENSP00000413255.2
            RefSeq Select criteria             :: based on manual assertion,
                                                  conservation
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Homo sapiens"
                     /db_xref="taxon:9606"
                     /chromosome="15"
                     /map="15q15.3"
     Protein         1..417
                     /product="creatine kinase U-type, mitochondrial precursor"
                     /EC_number="2.7.3.2"
                     /note="creatine kinase, mitochondrial 1 (ubiquitous);
                     creatine kinase U-type, mitochondrial; acidic-type
                     mitochondrial creatine kinase; ubiquitous mitochondrial
                     creatine kinase; U-MtCK; mia-CK"
                     /calculated_mol_wt=43080
     transit_peptide 1..39
                     /note="Mitochondrion; propagated from UniProtKB/Swiss-Prot
                     (P12532.1)"
                     /calculated_mol_wt=3975
     mat_peptide     40..417
                     /product="Creatine kinase U-type, mitochondrial.
                     /id=PRO_0000016590"
                     /note="propagated from UniProtKB/Swiss-Prot (P12532.1)"
                     /calculated_mol_wt=43080
     Region          40..64
                     /region_name="Cardiolipin-binding. /evidence=ECO:0000250"
                     /note="propagated from UniProtKB/Swiss-Prot (P12532.1)"
     Region          49..406
                     /region_name="creatine_kinase_like"
                     /note="Phosphagen (guanidino) kinases such as creatine
                     kinase and similar enzymes; cd00716"
                     /db_xref="CDD:153076"
     Site            order(105,234,265,316,318)
                     /site_type="other"
                     /note="creatine binding site [chemical binding]"
                     /db_xref="CDD:153076"
     Site            151
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:P25809; propagated from
                     UniProtKB/Swiss-Prot (P12532.1)"
     Site            order(161,163,165,224,261,269,325,327..329,353,355,
                     357..358,368)
                     /site_type="other"
                     /note="ADP binding site [chemical binding]"
                     /db_xref="CDD:153076"
     Site            196
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:P25809; propagated from
                     UniProtKB/Swiss-Prot (P12532.1)"
     Site            213
                     /site_type="phosphorylation"
                     /note="Phosphothreonine.
                     /evidence=ECO:0000250|UniProtKB:P00564; propagated from
                     UniProtKB/Swiss-Prot (P12532.1)"
     Site            232
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:P30275; propagated from
                     UniProtKB/Swiss-Prot (P12532.1)"
     Site            order(350..359,362..371)
                     /site_type="active"
                     /note="substrate specificity loop [active]"
                     /db_xref="CDD:153076"
     Site            355
                     /site_type="phosphorylation"
                     /note="Phosphothreonine.
                     /evidence=ECO:0000250|UniProtKB:P07310; propagated from
                     UniProtKB/Swiss-Prot (P12532.1)"
     CDS             1..417
                     /gene="CKMT1B"
                     /gene_synonym="CKMT; CKMT1; UMTCK"
                     /coded_by="NM_001375484.1:193..1446"
                     /db_xref="CCDS:CCDS10097.1"
                     /db_xref="GeneID:1159"
                     /db_xref="HGNC:HGNC:1995"
                     /db_xref="MIM:123290"
ORIGIN      
        1 magpfsrlls arpglrllal agagslaagf llrpepvraa serrrlypps aeypdlrkhn
       61 ncmashltpa vyarlcdktt ptgwtldqci qtgvdnpghp fiktvgmvag deetyevfad
      121 lfdpviqerh ngydprtmkh ttdldaskir sgyfderyvl ssrvrtgrsi rglslppact
      181 raerreverv vvdalsglkg dlagryyrls emteaeqqql iddhflfdkp vsplltaagm
      241 ardwpdargi whnneksfli wvneedhtrv ismekggnmk rvferfcrgl keverliqer
      301 gwefmwnerl gyiltcpsnl gtglragvhi klpllskdsr fpkilenlrl qkrgtggvdt
      361 aatggvfdis nldrlgksev elvqlvidgv nylidcerrl ergqdiript pvihtkh
//
