LOCUS       NM_016951                656 bp    mRNA    linear   PRI 23-MAR-2024
DEFINITION  Homo sapiens chemokine like factor (CKLF), transcript variant 1,
            mRNA.
ACCESSION   NM_016951
VERSION     NM_016951.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Qin,X., Lam,A., Zhang,X., Sengupta,S., Iorgulescu,J.B., Ni,H.,
            Das,S., Rager,M., Zhou,Z., Zuo,T., Meara,G.K., Floru,A.E.,
            Kemet,C., Veerapaneni,D., Kashy,D., Lin,L., Lloyd,K., Kwok,L.,
            Smith,K.S., Nagaraju,R.T., Meijers,R., Ceol,C., Liu,C.T.,
            Alexandrescu,S., Wu,C.J., Keskin,D.B., George,R.E. and Feng,H.
  TITLE     CKLF instigates a 'cold' microenvironment to promote MYCN-mediated
            tumor aggressiveness
  JOURNAL   Sci Adv 10 (11), eadh9547 (2024)
   PUBMED   38489372
  REMARK    GeneRIF: CKLF instigates a 'cold' microenvironment to promote
            MYCN-mediated tumor aggressiveness.
REFERENCE   2  (bases 1 to 656)
  AUTHORS   Duan,S.L., Jiang,Y., Li,G.Q., Fu,W., Song,Z., Li,L.N. and Li,J.
  TITLE     Research insights into the chemokine-like factor (CKLF)-like MARVEL
            transmembrane domain-containing family (CMTM): their roles in
            various tumors
  JOURNAL   PeerJ 12, e16757 (2024)
   PUBMED   38223763
  REMARK    GeneRIF: Research insights into the chemokine-like factor
            (CKLF)-like MARVEL transmembrane domain-containing family (CMTM):
            their roles in various tumors.
            Review article
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 656)
  AUTHORS   Zhou,X., Zhang,Y.N., Li,F.F., Zhang,Z., Cui,L.Y., He,H.Y., Yan,X.,
            He,W.B., Sun,H.S., Feng,Z.P., Chu,S.F. and Chen,N.H.
  TITLE     Neuronal chemokine-like-factor 1 (CKLF1) up-regulation promotes M1
            polarization of microglia in rat brain after stroke
  JOURNAL   Acta Pharmacol Sin 43 (5), 1217-1230 (2022)
   PUBMED   34385606
  REMARK    GeneRIF: Neuronal chemokine-like-factor 1 (CKLF1) up-regulation
            promotes M1 polarization of microglia in rat brain after stroke.
REFERENCE   4  (bases 1 to 656)
  AUTHORS   Fitter,S., Bradey,A.L., Kok,C.H., Noll,J.E., Wilczek,V.J.,
            Venn,N.C., Law,T., Paisitkriangkrai,S., Story,C., Saunders,L.,
            Dalla Pozza,L., Marshall,G.M., White,D.L., Sutton,R.,
            Zannettino,A.C.W. and Revesz,T.
  TITLE     CKLF and IL1B transcript levels at diagnosis are predictive of
            relapse in children with pre-B-cell acute lymphoblastic leukaemia
  JOURNAL   Br J Haematol 193 (1), 171-175 (2021)
   PUBMED   33620089
  REMARK    GeneRIF: CKLF and IL1B transcript levels at diagnosis are
            predictive of relapse in children with pre-B-cell acute
            lymphoblastic leukaemia.
REFERENCE   5  (bases 1 to 656)
  AUTHORS   Chen,C., Ai,Q.D. and Wei,Y.H.
  TITLE     Kanglaite enhances the efficacy of cisplatin in suppression of
            hepatocellular carcinoma via inhibiting CKLF1 mediated NF-kappaB
            pathway and regulating transporter mediated drug efflux
  JOURNAL   J Ethnopharmacol 264, 113388 (2021)
   PUBMED   32918990
  REMARK    GeneRIF: Kanglaite enhances the efficacy of cisplatin in
            suppression of hepatocellular carcinoma via inhibiting CKLF1
            mediated NF-kappaB pathway and regulating transporter mediated drug
            efflux.
REFERENCE   6  (bases 1 to 656)
  AUTHORS   Wang,Y., Zhang,Y., Han,W., Li,D., Tian,L., Yin,C. and Ma,D.
  TITLE     Two C-terminal peptides of human CKLF1 interact with the chemokine
            receptor CCR4
  JOURNAL   Int J Biochem Cell Biol 40 (5), 909-919 (2008)
   PUBMED   18069042
  REMARK    GeneRIF: Results confirm that the secreted peptides of CKLF1, C27
            and C19, have functional activation via CCR4.
REFERENCE   7  (bases 1 to 656)
  AUTHORS   Xu,M., Han,W., Qian,M., Ma,X., Ding,P., Wang,Y., Xia,D., Rui,M.,
            Wang,L., Zhang,Y. and Ma,D.
  TITLE     Last intron of the chemokine-like factor gene contains a putative
            promoter for the downstream CKLF super family member 1 gene
  JOURNAL   Biochem Biophys Res Commun 313 (1), 135-141 (2004)
   PUBMED   14672709
REFERENCE   8  (bases 1 to 656)
  AUTHORS   Xia,D., Li,X., Lou,Y., Han,W., Ding,P., Zhang,Y., Di,C., Song,Q.
            and Ma,D.
  TITLE     Overexpression of chemokine-like factor 2 promotes the
            proliferation and survival of C2C12 skeletal muscle cells
  JOURNAL   Biochim Biophys Acta 1591 (1-3), 163-173 (2002)
   PUBMED   12183067
REFERENCE   9  (bases 1 to 656)
  AUTHORS   Ke,X., Jia,L., Jing,H., Liu,Y., Zhang,Y. and Di,C.
  TITLE     Effects of novel human chemokine-like factor 1 (CKLF1) on bone
            marrow hematopoietic stem cell/progenitor cell in vitro
  JOURNAL   Zhonghua Xue Ye Xue Za Zhi 23 (6), 301-303 (2002)
   PUBMED   12411060
  REMARK    GeneRIF: CKLF1 could increase the proliferation of human bone
            marrow hematopoietic progenitor cells and colony formation
REFERENCE   10 (bases 1 to 656)
  AUTHORS   Han,W., Lou,Y., Tang,J., Zhang,Y., Chen,Y., Li,Y., Gu,W., Huang,J.,
            Gui,L., Tang,Y., Li,F., Song,Q., Di,C., Wang,L., Shi,Q., Sun,R.,
            Xia,D., Rui,M., Tang,J. and Ma,D.
  TITLE     Molecular cloning and characterization of chemokine-like factor 1
            (CKLF1), a novel human cytokine with unique structure and potential
            chemotactic activity
  JOURNAL   Biochem J 357 (Pt 1), 127-135 (2001)
   PUBMED   11415443
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AF135380.2.
            
            On Nov 30, 2019 this sequence version replaced NM_016951.3.
            
            Summary: The product of this gene is a cytokine. Cytokines are
            small proteins that have an essential role in the immune and
            inflammatory responses. This gene is one of several chemokine-like
            factor genes located in a cluster on chromosome 16. The protein
            encoded by this gene is a potent chemoattractant for neutrophils,
            monocytes and lymphocytes. It also can stimulate the proliferation
            of skeletal muscle cells. This protein may play important roles in
            inflammation and in the regeneration of skeletal muscle.
            Alternatively spliced transcript variants encoding different
            isoforms have been identified. Naturally occurring read-through
            transcription occurs between this locus and the neighboring locus
            CMTM1 (CKLF-like MARVEL transmembrane domain containing
            1).[provided by RefSeq, Feb 2011].
            
            Transcript Variant: This variant (1) represents the longest
            transcript, and encodes the longest isoform (a), also known as an
            isoform CKLF2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BI561562.1, AF135380.2 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000264001.9/ ENSP00000264001.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-656               AF135380.2         8-663
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="16"
                     /map="16q21"
     gene            1..656
                     /gene="CKLF"
                     /gene_synonym="C32; CKLF1; CKLF2; CKLF3; CKLF4; HSPC224;
                     UCK-1"
                     /note="chemokine like factor"
                     /db_xref="GeneID:51192"
                     /db_xref="HGNC:HGNC:13253"
                     /db_xref="MIM:616074"
     exon            1..218
                     /gene="CKLF"
                     /gene_synonym="C32; CKLF1; CKLF2; CKLF3; CKLF4; HSPC224;
                     UCK-1"
                     /inference="alignment:Splign:2.1.0"
     CDS             141..599
                     /gene="CKLF"
                     /gene_synonym="C32; CKLF1; CKLF2; CKLF3; CKLF4; HSPC224;
                     UCK-1"
                     /note="isoform a is encoded by transcript variant 1;
                     transmembrane proteolipid; chemokine-like factor 1;
                     chemokine-like factor 2; chemokine-like factor 3;
                     chemokine-like factor 4"
                     /codon_start=1
                     /product="chemokine-like factor isoform a"
                     /protein_id="NP_058647.1"
                     /db_xref="CCDS:CCDS10807.1"
                     /db_xref="GeneID:51192"
                     /db_xref="HGNC:HGNC:13253"
                     /db_xref="MIM:616074"
                     /translation="MDNVQPKIKHRPFCFSVKGHVKMLRLALTVTSMTFFIIAQAPEP
                     YIVITGFEVTVILFFILLYVLRLDRLMKWLFWPLLDIINSLVTTVFMLIVSVLALIPE
                     TTTLTVGGGVFALVTAVCCLADGALIYRKLLFNPSGPYQKKPVHEKKEVL"
     exon            219..377
                     /gene="CKLF"
                     /gene_synonym="C32; CKLF1; CKLF2; CKLF3; CKLF4; HSPC224;
                     UCK-1"
                     /inference="alignment:Splign:2.1.0"
     exon            378..473
                     /gene="CKLF"
                     /gene_synonym="C32; CKLF1; CKLF2; CKLF3; CKLF4; HSPC224;
                     UCK-1"
                     /inference="alignment:Splign:2.1.0"
     exon            474..656
                     /gene="CKLF"
                     /gene_synonym="C32; CKLF1; CKLF2; CKLF3; CKLF4; HSPC224;
                     UCK-1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      631..636
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CKLF"
                     /gene_synonym="C32; CKLF1; CKLF2; CKLF3; CKLF4; HSPC224;
                     UCK-1"
                     /note="hexamer: ATTAAA"
     polyA_site      656
                     /gene="CKLF"
                     /gene_synonym="C32; CKLF1; CKLF2; CKLF3; CKLF4; HSPC224;
                     UCK-1"
                     /note="major polyA site"
ORIGIN      
        1 agcgggaagc cgagctgggc gagaagtagg ggagggcggt gctccgccgc ggtggcggtt
       61 gctatcgctt cgcagaacct actcaggcag ccagctgaga agagttgagg gaaagtgctg
      121 ctgctgggtc tgcagacgcg atggataacg tgcagccgaa aataaaacat cgccccttct
      181 gcttcagtgt gaaaggccac gtgaagatgc tgcggctggc actaactgtg acatctatga
      241 ccttttttat catcgcacaa gcccctgaac catatattgt tatcactgga tttgaagtca
      301 ccgttatctt atttttcata cttttatatg tactcagact tgatcgatta atgaagtggt
      361 tattttggcc tttgcttgat attatcaact cactggtaac aacagtattc atgctcatcg
      421 tatctgtgtt ggcactgata ccagaaacca caacattgac agttggtgga ggggtgtttg
      481 cacttgtgac agcagtatgc tgtcttgccg acggggccct tatttaccgg aagcttctgt
      541 tcaatcccag cggtccttac cagaaaaagc ctgtgcatga aaaaaaagaa gttttgtaat
      601 tttatattac tttttagttt gatactaagt attaaacata tttctgtatt cttcca
//
