LOCUS       NM_001008938            7172 bp    mRNA    linear   PRI 21-SEP-2024
DEFINITION  Homo sapiens cytoskeleton associated protein 5 (CKAP5), transcript
            variant 1, mRNA.
ACCESSION   NM_001008938
VERSION     NM_001008938.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7172)
  AUTHORS   Lakshmi,R.B., Nayak,P., Raz,L., Sarkar,A., Saroha,A., Kumari,P.,
            Nair,V.M., Kombarakkaran,D.P., Sajana,S., M,G. S, Agasti,S.S.,
            Paul,R., Ben-David,U. and Manna,T.K.
  TITLE     CKAP5 stabilizes CENP-E at kinetochores by regulating
            microtubule-chromosome attachments
  JOURNAL   EMBO Rep 25 (4), 1909-1935 (2024)
   PUBMED   38424231
  REMARK    GeneRIF: CKAP5 stabilizes CENP-E at kinetochores by regulating
            microtubule-chromosome attachments.
REFERENCE   2  (bases 1 to 7172)
  AUTHORS   Wang,Y., Ma,X., Wang,S., Li,Z., Wang,F., Tian,X., Fu,H., Xing,G.,
            Sun,L. and Wang,W.
  TITLE     Competing endogenous RNA analysis reveals the regulatory potency of
            CKAP5 in HPV+ HNSCC
  JOURNAL   Oral Dis 29 (8), 3152-3156 (2023)
   PUBMED   35710876
  REMARK    GeneRIF: Competing endogenous RNA analysis reveals the regulatory
            potency of CKAP5 in HPV+ HNSCC.
REFERENCE   3  (bases 1 to 7172)
  AUTHORS   Rajeev,R., Mukhopadhyay,S., Bhagyanath,S., Devu Priya,M.R.S. and
            Manna,T.K.
  TITLE     TACC3-ch-TOG interaction regulates spindle microtubule assembly by
            controlling centrosomal recruitment of gamma-TuRC
  JOURNAL   Biosci Rep 43 (3) (2023)
   PUBMED   36790370
  REMARK    GeneRIF: TACC3-ch-TOG interaction regulates spindle microtubule
            assembly by controlling centrosomal recruitment of gamma-TuRC.
REFERENCE   4  (bases 1 to 7172)
  AUTHORS   Rossi,F., Beltran,M., Damizia,M., Grelloni,C., Colantoni,A.,
            Setti,A., Di Timoteo,G., Dattilo,D., Centron-Broco,A.,
            Nicoletti,C., Fanciulli,M., Lavia,P. and Bozzoni,I.
  TITLE     Circular RNA ZNF609/CKAP5 mRNA interaction regulates microtubule
            dynamics and tumorigenicity
  JOURNAL   Mol Cell 82 (1), 75-89 (2022)
   PUBMED   34942120
  REMARK    GeneRIF: Circular RNA ZNF609/CKAP5 mRNA interaction regulates
            microtubule dynamics and tumorigenicity.
REFERENCE   5  (bases 1 to 7172)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   6  (bases 1 to 7172)
  AUTHORS   Lauffart,B., Howell,S.J., Tasch,J.E., Cowell,J.K. and Still,I.H.
  TITLE     Interaction of the transforming acidic coiled-coil 1 (TACC1)
            protein with ch-TOG and GAS41/NuBI1 suggests multiple
            TACC1-containing protein complexes in human cells
  JOURNAL   Biochem J 363 (Pt 1), 195-200 (2002)
   PUBMED   11903063
  REMARK    GeneRIF: Interaction of the transforming acidic coiled-coil 1
            (TACC1) protein with ch-TOG and GAS41/NuBI1 suggests multiple
            TACC1-containing protein complexes in human cells
REFERENCE   7  (bases 1 to 7172)
  AUTHORS   Mayor,T., Stierhof,Y.D., Tanaka,K., Fry,A.M. and Nigg,E.A.
  TITLE     The centrosomal protein C-Nap1 is required for cell cycle-regulated
            centrosome cohesion
  JOURNAL   J Cell Biol 151 (4), 837-846 (2000)
   PUBMED   11076968
REFERENCE   8  (bases 1 to 7172)
  AUTHORS   Spittle,C., Charrasse,S., Larroque,C. and Cassimeris,L.
  TITLE     The interaction of TOGp with microtubules and tubulin
  JOURNAL   J Biol Chem 275 (27), 20748-20753 (2000)
   PUBMED   10770946
REFERENCE   9  (bases 1 to 7172)
  AUTHORS   Charrasse,S., Mazel,M., Taviaux,S., Berta,P., Chow,T. and
            Larroque,C.
  TITLE     Characterization of the cDNA and pattern of expression of a new
            gene over-expressed in human hepatomas and colonic tumors
  JOURNAL   Eur J Biochem 234 (2), 406-413 (1995)
   PUBMED   8536682
REFERENCE   10 (bases 1 to 7172)
  AUTHORS   Golsteyn,R.M., Mundt,K.E., Fry,A.M. and Nigg,E.A.
  TITLE     Cell cycle regulation of the activity and subcellular localization
            of Plk1, a human protein kinase implicated in mitotic spindle
            function
  JOURNAL   J Cell Biol 129 (6), 1617-1628 (1995)
   PUBMED   7790358
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BP282525.1, BC120869.1,
            D43948.1, CB052595.1, BM980015.1 and AC115088.6.
            This sequence is a reference standard in the RefSeqGene project.
            
            On May 17, 2019 this sequence version replaced NM_001008938.3.
            
            Summary: This gene encodes a cytoskeleton-associated protein which
            belongs to the TOG/XMAP215 family. The N-terminal half of this
            protein contains a microtubule-binding domain and the C-terminal
            half contains a KXGS motif for binding tubulin dimers. This protein
            has two distinct roles in spindle formation; it protects
            kinetochore microtubules from depolymerization and plays an
            essential role in centrosomal microtubule assembly. This protein
            may be necessary for the proper interaction of microtubules with
            the cell cortex for directional cell movement. It also plays a role
            in translation of the myelin basic protein (MBP) mRNA by
            interacting with heterogeneous nuclear ribonucleoprotein (hnRNP)
            A2, which associates with MBP. Alternatively spliced transcript
            variants encoding different isoforms have been identified.
            [provided by RefSeq, Aug 2011].
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803614.45127.1,
                                           SRR1803612.265289.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000529230.6/ ENSP00000432768.1
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-78                BP282525.1         29-106
            79-3817             BC120869.1         1-3739
            3818-6480           D43948.1           3767-6429
            6481-6677           CB052595.1         26-222              c
            6678-6686           BM980015.1         17-25               c
            6687-7172           AC115088.6         103570-104055       c
FEATURES             Location/Qualifiers
     source          1..7172
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11p11.2"
     gene            1..7172
                     /gene="CKAP5"
                     /gene_synonym="ch-TOG; CHTOG; MSPS; TOG; TOGp"
                     /note="cytoskeleton associated protein 5"
                     /db_xref="GeneID:9793"
                     /db_xref="HGNC:HGNC:28959"
                     /db_xref="MIM:611142"
     exon            1..61
                     /gene="CKAP5"
                     /gene_synonym="ch-TOG; CHTOG; MSPS; TOG; TOGp"
                     /inference="alignment:Splign:2.1.0"
     exon            62..155
                     /gene="CKAP5"
                     /gene_synonym="ch-TOG; CHTOG; MSPS; TOG; TOGp"
                     /inference="alignment:Splign:2.1.0"
     CDS             99..6197
                     /gene="CKAP5"
                     /gene_synonym="ch-TOG; CHTOG; MSPS; TOG; TOGp"
                     /note="isoform a is encoded by transcript variant 1;
                     colonic and hepatic tumor over-expressed gene protein;
                     colonic and hepatic tumor overexpressed gene protein"
                     /codon_start=1
                     /product="cytoskeleton-associated protein 5 isoform a"
                     /protein_id="NP_001008938.1"
                     /db_xref="CCDS:CCDS31477.1"
                     /db_xref="GeneID:9793"
                     /db_xref="HGNC:HGNC:28959"
                     /db_xref="MIM:611142"
                     /translation="MGDDSEWLKLPVDQKCEHKLWKARLSGYEEALKIFQKIKDEKSP
                     EWSKFLGLIKKFVTDSNAVVQLKGLEAALVYVENAHVAGKTTGEVVSGVVSKVFNQPK
                     AKAKELGIEICLMYIEIEKGEAVQEELLKGLDNKNPKIIVACIETLRKALSEFGSKII
                     LLKPIIKVLPKLFESREKAVRDEAKLIAVEIYRWIRDALRPPLQNINSVQLKELEEEW
                     VKLPTSAPRPTRFLRSQQELEAKLEQQQSAGGDAEGGGDDGDEVPQIDAYELLEAVEI
                     LSKLPKDFYDKIEAKKWQERKEALESVEVLIKNPKLEAGDYADLVKALKKVVGKDTNV
                     MLVALAAKCLTGLAVGLRKKFGQYAGHVVPTILEKFKEKKPQVVQALQEAIDAIFLTT
                     TLQNISEDVLAVMDNKNPTIKQQTSLFIARSFRHCTASTLPKSLLKPFCAALLKHIND
                     SAPEVRDAAFEALGTALKVVGEKAVNPFLADVDKLKLDKIKECSEKVELIHGKKAGLA
                     ADKKEFKPLPGRTAASGAAGDKDTKDISAPKPGPLKKAPAAKAGGPPKKGKPAAPGGA
                     GNTGTKNKKGLETKEIVEPELSIEVCEEKASAVLPPTCIQLLDSSNWKERLACMEEFQ
                     KAVELMDRTEMPCQALVRMLAKKPGWKETNFQVMQMKLHIVALIAQKGNFSKTSAQVV
                     LDGLVDKIGDVKCGNNAKEAMTAIAEACMLPWTAEQVVSMAFSQKNPKNQSETLNWLS
                     NAIKEFGFSGLNVKAFISNVKTALAATNPAVRTAAITLLGVMYLYVGPSLRMFFEDEK
                     PALLSQIDAEFEKMQGQSPPAPTRGISKHSTSGTDEGEDGDEPDDGSNDVVDLLPRTE
                     ISDKITSELVSKIGDKNWKIRKEGLDEVAGIINDAKFIQPNIGELPTALKGRLNDSNK
                     ILVQQTLNILQQLAVAMGPNIKQHVKNLGIPIITVLGDSKNNVRAAALATVNAWAEQT
                     GMKEWLEGEDLSEELKKENPFLRQELLGWLAEKLPTLRSTPTDLILCVPHLYSCLEDR
                     NGDVRKKAQDALPFFMMHLGYEKMAKATGKLKPTSKDQVLAMLEKAKVNMPAKPAPPT
                     KATSKPMGGSAPAKFQPASAPAEDCISSSTEPKPDPKKAKAPGLSSKAKSAQGKKMPS
                     KTSLKEDEDKSGPIFIVVPNGKEQRMKDEKGLKVLKWNFTTPRDEYIEQLKTQMSSCV
                     AKWLQDEMFHSDFQHHNKALAVMVDHLESEKEGVIGCLDLILKWLTLRFFDTNTSVLM
                     KALEYLKLLFTLLSEEEYHLTENEASSFIPYLVVKVGEPKDVIRKDVRAILNRMCLVY
                     PASKMFPFIMEGTKSKNSKQRAECLEELGCLVESYGMNVCQPTPGKALKEIAVHIGDR
                     DNAVRNAALNTIVTVYNVHGDQVFKLIGNLSEKDMSMLEERIKRSAKRPSAAPIKQVE
                     EKPQRAQNISSNANMLRKGPAEDMSSKLNQARSMSGHPEAAQMVRREFQLDLDEIEND
                     NGTVRCEMPELVQHKLDDIFEPVLIPEPKIRAVSPHFDDMHSNTASTINFIISQVASG
                     DINTSIQALTQIDEVLRQEDKAEAMSGHIDQFLIATFMQLRLIYNTHMADEKLEKDEI
                     IKLYSCIIGNMISLFQIESLAREASTGVLKDLMHGLITLMLDSRIEDLEEGQQVIRSV
                     NLLVVKVLEKSDQTNILSALLVLLQDSLLATASSPKFSELVMKCLWRMVRLLPDTINS
                     INLDRILLDIHIFMKVFPKEKLKQCKSEFPIRTLKTLLHTLCKLKGPKILDHLTMIDN
                     KNESELEAHLCRMMKHSMDQTGSKSDKETEKGASRIDEKSSKAKVNDFLAEIFKKIGS
                     KENTKEGLAELYEYKKKYSDADIEPFLKNSSQFFQSYVERGLRVIEMEREGKGRISTS
                     TGISPQMEVTCVPTPTSTVSSIGNTNGEEVGPSVYLERLKILRQRCGLDNTKQDDRPP
                     LTSLLSKPAVPTVASSTDMLHSKLSQLRESREQHQHSDLDSNQTHSSGTVTSSSSTAN
                     IDDLKKRLERIKSSRK"
     exon            156..349
                     /gene="CKAP5"
                     /gene_synonym="ch-TOG; CHTOG; MSPS; TOG; TOGp"
                     /inference="alignment:Splign:2.1.0"
     exon            350..556
                     /gene="CKAP5"
                     /gene_synonym="ch-TOG; CHTOG; MSPS; TOG; TOGp"
                     /inference="alignment:Splign:2.1.0"
     exon            557..728
                     /gene="CKAP5"
                     /gene_synonym="ch-TOG; CHTOG; MSPS; TOG; TOGp"
                     /inference="alignment:Splign:2.1.0"
     exon            729..861
                     /gene="CKAP5"
                     /gene_synonym="ch-TOG; CHTOG; MSPS; TOG; TOGp"
                     /inference="alignment:Splign:2.1.0"
     exon            862..962
                     /gene="CKAP5"
                     /gene_synonym="ch-TOG; CHTOG; MSPS; TOG; TOGp"
                     /inference="alignment:Splign:2.1.0"
     exon            963..1076
                     /gene="CKAP5"
                     /gene_synonym="ch-TOG; CHTOG; MSPS; TOG; TOGp"
                     /inference="alignment:Splign:2.1.0"
     exon            1077..1181
                     /gene="CKAP5"
                     /gene_synonym="ch-TOG; CHTOG; MSPS; TOG; TOGp"
                     /inference="alignment:Splign:2.1.0"
     exon            1182..1271
                     /gene="CKAP5"
                     /gene_synonym="ch-TOG; CHTOG; MSPS; TOG; TOGp"
                     /inference="alignment:Splign:2.1.0"
     exon            1272..1436
                     /gene="CKAP5"
                     /gene_synonym="ch-TOG; CHTOG; MSPS; TOG; TOGp"
                     /inference="alignment:Splign:2.1.0"
     exon            1437..1565
                     /gene="CKAP5"
                     /gene_synonym="ch-TOG; CHTOG; MSPS; TOG; TOGp"
                     /inference="alignment:Splign:2.1.0"
     exon            1566..1748
                     /gene="CKAP5"
                     /gene_synonym="ch-TOG; CHTOG; MSPS; TOG; TOGp"
                     /inference="alignment:Splign:2.1.0"
     exon            1749..1862
                     /gene="CKAP5"
                     /gene_synonym="ch-TOG; CHTOG; MSPS; TOG; TOGp"
                     /inference="alignment:Splign:2.1.0"
     exon            1863..1973
                     /gene="CKAP5"
                     /gene_synonym="ch-TOG; CHTOG; MSPS; TOG; TOGp"
                     /inference="alignment:Splign:2.1.0"
     exon            1974..2066
                     /gene="CKAP5"
                     /gene_synonym="ch-TOG; CHTOG; MSPS; TOG; TOGp"
                     /inference="alignment:Splign:2.1.0"
     exon            2067..2252
                     /gene="CKAP5"
                     /gene_synonym="ch-TOG; CHTOG; MSPS; TOG; TOGp"
                     /inference="alignment:Splign:2.1.0"
     exon            2253..2347
                     /gene="CKAP5"
                     /gene_synonym="ch-TOG; CHTOG; MSPS; TOG; TOGp"
                     /inference="alignment:Splign:2.1.0"
     exon            2348..2405
                     /gene="CKAP5"
                     /gene_synonym="ch-TOG; CHTOG; MSPS; TOG; TOGp"
                     /inference="alignment:Splign:2.1.0"
     exon            2406..2531
                     /gene="CKAP5"
                     /gene_synonym="ch-TOG; CHTOG; MSPS; TOG; TOGp"
                     /inference="alignment:Splign:2.1.0"
     exon            2532..2671
                     /gene="CKAP5"
                     /gene_synonym="ch-TOG; CHTOG; MSPS; TOG; TOGp"
                     /inference="alignment:Splign:2.1.0"
     exon            2672..2846
                     /gene="CKAP5"
                     /gene_synonym="ch-TOG; CHTOG; MSPS; TOG; TOGp"
                     /inference="alignment:Splign:2.1.0"
     exon            2847..2960
                     /gene="CKAP5"
                     /gene_synonym="ch-TOG; CHTOG; MSPS; TOG; TOGp"
                     /inference="alignment:Splign:2.1.0"
     exon            2961..3089
                     /gene="CKAP5"
                     /gene_synonym="ch-TOG; CHTOG; MSPS; TOG; TOGp"
                     /inference="alignment:Splign:2.1.0"
     exon            3090..3284
                     /gene="CKAP5"
                     /gene_synonym="ch-TOG; CHTOG; MSPS; TOG; TOGp"
                     /inference="alignment:Splign:2.1.0"
     exon            3285..3420
                     /gene="CKAP5"
                     /gene_synonym="ch-TOG; CHTOG; MSPS; TOG; TOGp"
                     /inference="alignment:Splign:2.1.0"
     exon            3421..3509
                     /gene="CKAP5"
                     /gene_synonym="ch-TOG; CHTOG; MSPS; TOG; TOGp"
                     /inference="alignment:Splign:2.1.0"
     exon            3510..3635
                     /gene="CKAP5"
                     /gene_synonym="ch-TOG; CHTOG; MSPS; TOG; TOGp"
                     /inference="alignment:Splign:2.1.0"
     exon            3636..3785
                     /gene="CKAP5"
                     /gene_synonym="ch-TOG; CHTOG; MSPS; TOG; TOGp"
                     /inference="alignment:Splign:2.1.0"
     exon            3786..3989
                     /gene="CKAP5"
                     /gene_synonym="ch-TOG; CHTOG; MSPS; TOG; TOGp"
                     /inference="alignment:Splign:2.1.0"
     exon            3990..4125
                     /gene="CKAP5"
                     /gene_synonym="ch-TOG; CHTOG; MSPS; TOG; TOGp"
                     /inference="alignment:Splign:2.1.0"
     exon            4126..4319
                     /gene="CKAP5"
                     /gene_synonym="ch-TOG; CHTOG; MSPS; TOG; TOGp"
                     /inference="alignment:Splign:2.1.0"
     exon            4320..4492
                     /gene="CKAP5"
                     /gene_synonym="ch-TOG; CHTOG; MSPS; TOG; TOGp"
                     /inference="alignment:Splign:2.1.0"
     exon            4493..4666
                     /gene="CKAP5"
                     /gene_synonym="ch-TOG; CHTOG; MSPS; TOG; TOGp"
                     /inference="alignment:Splign:2.1.0"
     exon            4667..4787
                     /gene="CKAP5"
                     /gene_synonym="ch-TOG; CHTOG; MSPS; TOG; TOGp"
                     /inference="alignment:Splign:2.1.0"
     exon            4788..4967
                     /gene="CKAP5"
                     /gene_synonym="ch-TOG; CHTOG; MSPS; TOG; TOGp"
                     /inference="alignment:Splign:2.1.0"
     exon            4968..5155
                     /gene="CKAP5"
                     /gene_synonym="ch-TOG; CHTOG; MSPS; TOG; TOGp"
                     /inference="alignment:Splign:2.1.0"
     exon            5156..5231
                     /gene="CKAP5"
                     /gene_synonym="ch-TOG; CHTOG; MSPS; TOG; TOGp"
                     /inference="alignment:Splign:2.1.0"
     exon            5232..5420
                     /gene="CKAP5"
                     /gene_synonym="ch-TOG; CHTOG; MSPS; TOG; TOGp"
                     /inference="alignment:Splign:2.1.0"
     exon            5421..5558
                     /gene="CKAP5"
                     /gene_synonym="ch-TOG; CHTOG; MSPS; TOG; TOGp"
                     /inference="alignment:Splign:2.1.0"
     exon            5559..5642
                     /gene="CKAP5"
                     /gene_synonym="ch-TOG; CHTOG; MSPS; TOG; TOGp"
                     /inference="alignment:Splign:2.1.0"
     exon            5643..5802
                     /gene="CKAP5"
                     /gene_synonym="ch-TOG; CHTOG; MSPS; TOG; TOGp"
                     /inference="alignment:Splign:2.1.0"
     exon            5803..5954
                     /gene="CKAP5"
                     /gene_synonym="ch-TOG; CHTOG; MSPS; TOG; TOGp"
                     /inference="alignment:Splign:2.1.0"
     exon            5955..7172
                     /gene="CKAP5"
                     /gene_synonym="ch-TOG; CHTOG; MSPS; TOG; TOGp"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6659..6664
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CKAP5"
                     /gene_synonym="ch-TOG; CHTOG; MSPS; TOG; TOGp"
                     /note="hexamer: AATAAA"
     polyA_site      6685
                     /gene="CKAP5"
                     /gene_synonym="ch-TOG; CHTOG; MSPS; TOG; TOGp"
                     /note="major polyA site"
ORIGIN      
        1 gcggcggccc aagcggtttc aaacggctta gagcaggccg cttggttctg acccagctga
       61 ggaaatactc ttaattctaa ggaaaacctg gaagcacaat gggagatgac agtgagtggt
      121 tgaaactgcc agttgatcag aaatgtgaac acaagctgtg gaaagcaagg ttaagtgggt
      181 atgaagaggc cctgaagatc ttccagaaaa taaaggatga aaagagccca gagtggtcca
      241 aatttttagg attgatcaaa aaatttgtca ctgattccaa tgcagtggtt caattgaaag
      301 gattagaagc tgcacttgtt tatgttgaaa atgcccatgt agcaggaaaa accacaggag
      361 aagttgtgtc aggtgttgta agtaaggtgt tcaatcaacc taaagctaaa gccaaggagc
      421 tgggcataga gatctgtctt atgtacatag agattgagaa aggagaggct gttcaagaag
      481 agctcctgaa aggcttggac aataagaatc ccaagatcat agtggcctgt atagagacac
      541 tgaggaaagc cttaagtgaa tttggttcca aaatcatctt gcttaagcca attatcaaag
      601 tgttgccaaa actctttgag tctcgagaga aggctgttcg agatgaagcc aaactaattg
      661 ctgtggagat ttacagatgg attcgggatg ctctgagacc cccattacaa aatataaact
      721 ctgttcagtt gaaagaacta gaagaagaat gggtcaaact gccaacaagt gctcctagac
      781 ctactcgatt tcttcgttcc caacaagaac tagaagctaa attggaacaa caacagtctg
      841 ctggtggaga tgctgaagga ggtggtgatg atggtgatga ggtgccacaa atagatgctt
      901 atgagctttt agaagctgta gaaatccttt ccaaacttcc caaagacttt tatgacaaaa
      961 ttgaggcaaa aaaatggcaa gagagaaaag aggccctgga gtctgtagaa gtactaataa
     1021 aaaaccccaa actggaagct ggcgattatg cagatttagt aaaagcatta aagaaggttg
     1081 ttggaaagga caccaatgtc atgttggtgg ctttggcagc aaaatgtctt actggcctgg
     1141 ctgttgggct aaggaagaaa tttggacaat atgcaggaca tgttgtgcca accatcttgg
     1201 agaaattcaa agagaagaaa cctcaagtgg tacaagccct gcaggaggca attgatgcaa
     1261 tcttccttac taccacacta cagaacatca gtgaggatgt tttagcagta atggataata
     1321 aaaatccaac catcaagcag cagacatctc tttttattgc aagaagtttc cgccactgca
     1381 ctgcttctac cctgccaaag agcttgctaa agcccttttg tgctgcacta cttaagcaca
     1441 tcaatgattc tgctcctgaa gtcagagatg ccgcatttga agcattgggt actgctttga
     1501 aggtggttgg cgagaaagca gtaaacccat tcctagctga tgtggacaaa ctcaagcttg
     1561 ataagatcaa agaatgttca gaaaaggtag aactgataca tggtaagaaa gctggactag
     1621 ctgctgataa gaaggaattc aaacctctgc ctggaaggac tgctgcttca ggggctgcag
     1681 gagataagga cacaaaggac atttctgcac ccaaaccagg acctctaaaa aaggcacctg
     1741 ctgctaaggc tggtgggcca ccaaaaaagg ggaaaccagc tgcaccagga ggcgcaggga
     1801 atactggaac caagaacaag aaaggactgg agactaaaga aatagtggag cctgagctct
     1861 cgatagaagt atgtgaagaa aaagcttcag ctgttcttcc ccctacctgt atacagcttc
     1921 ttgacagcag taactggaaa gaaaggctgg cttgtatgga agagttccag aaggctgttg
     1981 agctaatgga ccgaactgaa atgccatgcc aggcattagt gaggatgcta gccaagaaac
     2041 ctggatggaa agaaactaat tttcaggtga tgcaaatgaa gcttcatata gttgctttga
     2101 ttgcccagaa gggaaatttt tccaaaacgt cagctcaggt tgtattagat ggccttgtgg
     2161 acaagattgg agatgtgaaa tgtgggaaca atgcaaaaga agctatgaca gcaatagccg
     2221 aagcctgtat gttaccatgg actgctgaac aggttgtgtc aatggctttc tcacaaaaga
     2281 atcccaaaaa tcagtcagaa actctgaatt ggctatcaaa tgccataaaa gaatttggtt
     2341 tttctgggtt gaatgtcaaa gctttcatta gcaatgtgaa gacagctctt gctgcaacaa
     2401 acccagctgt gaggactgct gccataaccc tgcttggcgt gatgtatctg tatgttggtc
     2461 cctctttgcg aatgttcttt gaggatgaga agcctgccct cctatcccag atagatgcag
     2521 aatttgagaa gatgcaggga caaagtccac ctgctccaac cagaggaatt tccaagcata
     2581 gcacaagtgg tacagatgaa ggagaagatg gagatgaacc agatgacggg agcaatgatg
     2641 tcgttgatct tttgccgagg acggagatca gtgataaaat cacttcagag ttggtatcta
     2701 agattggtga taagaattgg aagattagga aagaaggcct agatgaagtg gcaggtatta
     2761 ttaatgacgc aaaatttatc caaccgaata taggtgaact tccaactgcc ttgaagggtc
     2821 gactcaatga ttcaaataaa atcttggtac agcaaacgct gaatatcctg caacaactgg
     2881 cagtagccat gggcccaaat attaagcaac atgtaaaaaa tttaggcatc cctatcatca
     2941 cagtccttgg agacagcaag aacaatgttc gagctgctgc cctagcgact gtgaatgctt
     3001 gggcagaaca gactggcatg aaggaatggc tggaaggaga agatctttct gaagagctca
     3061 aaaaggaaaa tcctttcttg aggcaagagc ttctgggctg gctggctgag aaactaccta
     3121 ctcttcgttc cacccctaca gaccttatcc tttgtgttcc tcatctctac tcctgcctag
     3181 aagatcgaaa tggagatgtg cgaaagaagg cccaagatgc cttgccattc ttcatgatgc
     3241 atttaggata tgaaaaaatg gccaaggcta ctgggaaact aaagccaact tctaaagatc
     3301 aggtattggc catgctagag aaagccaaag ttaacatgcc agccaagcct gctccaccca
     3361 ctaaagcaac ttctaaacca atgggagggt ccgctccagc caaattccag cctgcatcag
     3421 cacctgctga agattgtatt tccagcagta cagaacccaa acctgatcca aaaaaggcca
     3481 aagctccagg attatcctct aaagcaaaga gtgcacaagg gaagaagatg ccaagcaaaa
     3541 ccagcttaaa ggaggatgaa gacaaatccg ggcctatttt tattgttgtt ccaaatggaa
     3601 aagagcaaag gatgaaagat gaaaaaggat tgaaggtgct aaagtggaat tttactaccc
     3661 cacgggatga atacattgag caactaaaga ctcaaatgtc tagctgtgtg gctaaatggt
     3721 tacaagatga gatgtttcac tcagactttc agcatcataa caaagccctt gctgttatgg
     3781 ttgatcactt ggagagtgaa aaagaaggag ttattggttg cctggatctt atcttaaagt
     3841 ggcttaccct gaggtttttt gacaccaata caagcgtcct gatgaaagca ctagaatatt
     3901 taaaattgct cttcaccttg ctaagtgaag aagaatatca tcttactgag aatgaagcat
     3961 cttccttcat cccctatctt gtcgtcaagg ttggagaacc aaaggatgtc attcgtaaag
     4021 atgttcgtgc catcctgaac cggatgtgcc ttgtctaccc agctagcaag atgtttccct
     4081 ttatcatgga aggaaccaaa tccaaaaact ctaagcagag agcagagtgc ctggaagagc
     4141 tgggatgtct ggttgagtcc tatggcatga atgtttgcca accaacccca ggaaaagcct
     4201 taaaggaaat agctgttcac ataggagacc gtgacaatgc tgtacgcaat gctgcactca
     4261 acaccattgt aacggtgtac aatgtacatg gggatcaggt gttcaaactg attggaaatc
     4321 tttctgaaaa ggatatgagc atgctcgagg agaggattaa gcggtcagca aagagaccct
     4381 ctgctgcacc aataaaacag gtggaagaga aacctcagcg tgcacagaac ataagctcca
     4441 atgccaacat gttacgcaag ggaccagctg aggacatgtc ttccaaactc aaccaagccc
     4501 gaagcatgag tgggcatcct gaggcagccc agatggtccg ccgagaattc cagctggatc
     4561 tagatgagat tgagaatgac aatggtacag tccgatgtga aatgccagaa cttgttcagc
     4621 acaaactgga tgacattttt gagccagtcc ttattcctga acccaagatc cgggctgttt
     4681 ctccacactt cgatgacatg cacagtaata cagcatccac aatcaatttc attatctccc
     4741 aagtagccag tggtgacatc aacacaagta tccaagctct gacacagatc gatgaggtcc
     4801 tgagacagga agacaaagct gaagccatgt ccggccatat tgatcagttt ctgatagcca
     4861 cttttatgca gctaagactc atctacaaca cacacatggc agatgagaaa ttggagaagg
     4921 acgagatcat caagttgtat agctgtatca ttggcaacat gatttcgctg tttcagatag
     4981 agagccttgc ccgggaggcc tccactggag tactaaaaga cctaatgcat ggcctcatca
     5041 ccttaatgct ggattctcgg attgaagatc ttgaggaagg acaacaggtc atccgctctg
     5101 tgaacctctt ggtggtgaag gttctggaga agtcagacca gaccaacatc ctgagtgccc
     5161 tacttgtttt gctccaagac agcctgctag caacagccag ttctcccaaa ttctcagagc
     5221 ttgttatgaa gtgtctctgg agaatggttc gactgttgcc tgataccatc aatagcatta
     5281 acctagacag aattcttctg gatatccaca ttttcatgaa ggtcttcccc aaagagaaac
     5341 tgaagcaatg caaaagtgaa tttcccataa ggaccctaaa gaccctgcta cacaccttat
     5401 gcaaattaaa agggcccaag atcctggacc acctaacgat gatcgacaac aaaaacgagt
     5461 ctgagctgga ggcccatctc tgccggatga tgaagcacag tatggaccag actgggagca
     5521 agtctgataa ggaaacagaa aagggagcat ctcgaataga tgaaaaatca tcaaaggcca
     5581 aagtgaatga tttcttagct gagattttta agaagattgg ctctaaagaa aacactaaag
     5641 agggactagc agagttatat gaatataaga agaaatactc agatgctgac attgaaccat
     5701 ttctgaaaaa ttcctcacag ttcttccaga gctatgtcga aagaggcctt cgggtgattg
     5761 agatggagag ggagggcaaa ggtcgtattt ccacttcaac aggcatctcc cctcagatgg
     5821 aagtcacatg tgtgcccacg cccacaagca cagtgtcctc cataggtaac acaaatgggg
     5881 aagaagtggg gccatctgtc tacttggaaa ggctaaagat cctccgacag cgatgtggtc
     5941 tggacaacac aaagcaagat gaccgacctc ctttgacctc tttgctctcc aaaccagcag
     6001 ttcctactgt cgcctcttcc acagacatgc tccacagcaa actctctcag ctccgggagt
     6061 cacgggagca gcaccagcat tcagacctgg attctaacca gactcactct tcaggaactg
     6121 tgacctcctc ctcctccaca gctaacatag acgacttgaa aaaaagactg gagagaataa
     6181 agagcagtcg caaatgaagc tgccccactc ccccggcacc ctgcagcttt agtttactaa
     6241 actagaagtc ctcatagttt aaaatggcct cagcaggcct agtgtataca aactggttgt
     6301 atgtatcatg ccgtggagct agggggagga gtcattgtgg cacaagtatt tgtacatact
     6361 ctgcttctct ctgtcagcgt cctgctgctc tagaagactg tccgtggatg agtttagtgt
     6421 acagacttgt aaacagctgc cccctctctg ctcagtctag ttcccagatc cttttctttt
     6481 ctttttaatt gctcatttgt aaaattgtcc taatctttcc tagcttttta atagttaata
     6541 ttagaaactc tttaatagtt ttcctttcag tttgtgagct cttctctgtc gccctgaagg
     6601 gtcactgtat tctgtatgaa tgcatggcat gatacaacta atttaagagt cttttataaa
     6661 taaagtttgc attaactata cctgactccc aagatgctcc atatcatgga gtgttgggca
     6721 gcaatttttt aacttttggt accagctggg tattcagcca gttagagaaa ggttaattac
     6781 tgcttacaga gcttcccttt ttcttggctc acagacttgg agctgtcttt tattcctttg
     6841 gaatggaagg aactctcctt ccctgctctc cacaaacagc tcctagggag cagaaaaatc
     6901 tcatccttcc ttgttttgag attttggtca tgatttccct ccaattatca ccagtcgttt
     6961 caagaagctc ttgggcatcc taccactttg ctgaccaaag gcacacaaga ttgcgcccag
     7021 agattggctg ggcttcagtg gttctcacac tacaactaga tttgtgttca tttgactaat
     7081 ggagtttggg aaatcttagt ggtttgctgc ttggttgtat gagtctcttt tggtccaggc
     7141 taacagttgc ttcacattca gtcagagtaa gg
//
