LOCUS       NM_006825               3121 bp    mRNA    linear   PRI 09-FEB-2025
DEFINITION  Homo sapiens cytoskeleton associated protein 4 (CKAP4), mRNA.
ACCESSION   NM_006825
VERSION     NM_006825.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3121)
  AUTHORS   Peksoz,R., Disci,E., Laloglu,E., Yildirim,M., Agirman,E.,
            Hannarici,Z. and Atamanalp,S.S.
  TITLE     The clinical significance and diagnostic value of serum Dickkopf1
            and CKAP4 levels in patients with gastric cancer: a prospective
            study
  JOURNAL   Eur Rev Med Pharmacol Sci 27 (20), 10031-10040 (2023)
   PUBMED   37916373
  REMARK    GeneRIF: The clinical significance and diagnostic value of serum
            Dickkopf1 and CKAP4 levels in patients with gastric cancer: a
            prospective study.
            Erratum:[Eur Rev Med Pharmacol Sci. 2024 May;28(9):3291. doi:
            10.26355/eurrev_202405_36199.. PMID: 38766782]
REFERENCE   2  (bases 1 to 3121)
  AUTHORS   Cai,M., Wu,W., Deng,S., Yang,Q., Wu,H., Wang,H., Zhang,J., Feng,Q.,
            Shao,J., Zeng,Y. and Li,J.
  TITLE     Expression of cytoskeleton-associated protein 4 is associated with
            poor prognosis and metastasis in nasopharyngeal carcinoma
  JOURNAL   Exp Biol Med (Maywood) 248 (12), 1085-1094 (2023)
   PUBMED   37208923
  REMARK    GeneRIF: Expression of cytoskeleton-associated protein 4 is
            associated with poor prognosis and metastasis in nasopharyngeal
            carcinoma.
REFERENCE   3  (bases 1 to 3121)
  AUTHORS   Li,X., Wang,J., Zhu,S., Zheng,J., Xie,Y., Jiang,H., Guo,J.,
            Wang,Y., Peng,Z., Wang,M., Wang,J., Wang,S., Zhong,Y. and Liu,Z.
  TITLE     DKK1 activates noncanonical NF-kappaB signaling via IL-6-induced
            CKAP4 receptor in multiple myeloma
  JOURNAL   Blood Adv 5 (18), 3656-3667 (2021)
   PUBMED   34470047
  REMARK    GeneRIF: DKK1 activates noncanonical NF-kappaB signaling via
            IL-6-induced CKAP4 receptor in multiple myeloma.
REFERENCE   4  (bases 1 to 3121)
  AUTHORS   Luo,T., Ding,K., Ji,J., Zhang,X., Yang,X., Chen,A., Huang,B.,
            Zhang,D., Wang,J. and Li,X.
  TITLE     Cytoskeleton-associated protein 4 (CKAP4) promotes malignant
            progression of human gliomas through inhibition of the Hippo
            signaling pathway
  JOURNAL   J Neurooncol 154 (3), 275-283 (2021)
   PUBMED   34476666
  REMARK    GeneRIF: Cytoskeleton-associated protein 4 (CKAP4) promotes
            malignant progression of human gliomas through inhibition of the
            Hippo signaling pathway.
REFERENCE   5  (bases 1 to 3121)
  AUTHORS   Wang,Y., Yu,H., Xie,X., Deng,T., Ye,L., Wu,L., Ding,X., Yang,Z.,
            Zhu,Q., Li,J., Zheng,Y., Yu,Z. and Chen,G.
  TITLE     Plasmalemma vesicle-associated protein promotes angiogenesis in
            cholangiocarcinoma via the DKK1/CKAP4/PI3K signaling pathway
  JOURNAL   Oncogene 40 (25), 4324-4337 (2021)
   PUBMED   34079085
  REMARK    GeneRIF: Plasmalemma vesicle-associated protein promotes
            angiogenesis in cholangiocarcinoma via the DKK1/CKAP4/PI3K
            signaling pathway.
REFERENCE   6  (bases 1 to 3121)
  AUTHORS   Klopfenstein,D.R., Klumperman,J., Lustig,A., Kammerer,R.A.,
            Oorschot,V. and Hauri,H.P.
  TITLE     Subdomain-specific localization of CLIMP-63 (p63) in the
            endoplasmic reticulum is mediated by its luminal alpha-helical
            segment
  JOURNAL   J Cell Biol 153 (6), 1287-1300 (2001)
   PUBMED   11402071
REFERENCE   7  (bases 1 to 3121)
  AUTHORS   Mundy,D.I.
  TITLE     Protein palmitoylation in membrane trafficking
  JOURNAL   Biochem Soc Trans 23 (3), 572-576 (1995)
   PUBMED   8566419
  REMARK    GeneRIF: Purification and cloning of p62/p63.
            Review article
REFERENCE   8  (bases 1 to 3121)
  AUTHORS   Schweizer,A., Rohrer,J., Slot,J.W., Geuze,H.J. and Kornfeld,S.
  TITLE     Reassessment of the subcellular localization of p63
  JOURNAL   J Cell Sci 108 (Pt 6), 2477-2485 (1995)
   PUBMED   7673362
REFERENCE   9  (bases 1 to 3121)
  AUTHORS   Schweizer,A., Rohrer,J., Jeno,P., DeMaio,A., Buchman,T.G. and
            Hauri,H.P.
  TITLE     A reversibly palmitoylated resident protein (p63) of an ER-Golgi
            intermediate compartment is related to a circulatory shock
            resuscitation protein
  JOURNAL   J Cell Sci 104 (Pt 3), 685-694 (1993)
   PUBMED   8314870
REFERENCE   10 (bases 1 to 3121)
  AUTHORS   Mundy,D.I. and Warren,G.
  TITLE     Mitosis and inhibition of intracellular transport stimulate
            palmitoylation of a 62-kD protein
  JOURNAL   J Cell Biol 116 (1), 135-146 (1992)
   PUBMED   1730740
  REMARK    GeneRIF: The p62/p63 protein was first identified because it is
            palmitoylated during mitosis.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC079174.20 and X69910.2.
            
            On Nov 23, 2018 this sequence version replaced NM_006825.3.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data because no single transcript was available
            for the full length of the gene. The extent of this transcript is
            supported by transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: X69910.2, SRR3476690.538100.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000378026.5/ ENSP00000367265.4
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-85                AC079174.20        68197-68281
            86-1143             X69910.2           1-1058
            1144-3121           AC079174.20        76359-78336
FEATURES             Location/Qualifiers
     source          1..3121
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q23.3"
     gene            1..3121
                     /gene="CKAP4"
                     /gene_synonym="CLIMP-63; CLIMP63; ERGIC-63; p63"
                     /note="cytoskeleton associated protein 4"
                     /db_xref="GeneID:10970"
                     /db_xref="HGNC:HGNC:16991"
                     /db_xref="MIM:618595"
     exon            1..652
                     /gene="CKAP4"
                     /gene_synonym="CLIMP-63; CLIMP63; ERGIC-63; p63"
                     /inference="alignment:Splign:2.1.0"
     CDS             170..1978
                     /gene="CKAP4"
                     /gene_synonym="CLIMP-63; CLIMP63; ERGIC-63; p63"
                     /note="transmembrane protein (63kD), endoplasmic
                     reticulum/Golgi intermediate compartment; type-II
                     transmembrane protein p63; 63 kDa membrane protein; 63-kDa
                     cytoskeleton-linking membrane protein"
                     /codon_start=1
                     /product="cytoskeleton-associated protein 4"
                     /protein_id="NP_006816.2"
                     /db_xref="CCDS:CCDS9103.1"
                     /db_xref="GeneID:10970"
                     /db_xref="HGNC:HGNC:16991"
                     /db_xref="MIM:618595"
                     /translation="MPSAKQRGSKGGHGAASPSEKGAHPSGGADDVAKKPPPAPQQPP
                     PPPAPHPQQHPQQHPQNQAHGKGGHRGGGGGGGKSSSSSSASAAAAAAAASSSASCSR
                     RLGRALNFLFYLALVAAAAFSGWCVHHVLEEVQQVRRSHQDFSRQREELGQGLQGVEQ
                     KVQSLQATFGTFESILRSSQHKQDLTEKAVKQGESEVSRISEVLQKLQNEILKDLSDG
                     IHVVKDARERDFTSLENTVEERLTELTKSINDNIAIFTEVQKRSQKEINDMKAKVASL
                     EESEGNKQDLKALKEAVKEIQTSAKSREWDMEALRSTLQTMESDIYTEVRELVSLKQE
                     QQAFKEAADTERLALQALTEKLLRSEESVSRLPEEIRRLEEELRQLKSDSHGPKEDGG
                     FRHSEAFEALQQKSQGLDSRLQHVEDGVLSMQVASARQTESLESLLSKSQEHEQRLAA
                     LQGRLEGLGSSEADQDGLASTVRSLGETQLVLYGDVEELKRSVGELPSTVESLQKVQE
                     QVHTLLSQDQAQAARLPPQDFLDRLSSLDNLKASVSQVEADLKMLRTAVDSLVAYSVK
                     IETNENNLESAKGLLDDLRNDLDRLFVKVEKIHEKV"
     exon            653..3121
                     /gene="CKAP4"
                     /gene_synonym="CLIMP-63; CLIMP63; ERGIC-63; p63"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3097..3102
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CKAP4"
                     /gene_synonym="CLIMP-63; CLIMP63; ERGIC-63; p63"
                     /note="hexamer: AATAAA"
     polyA_site      3121
                     /gene="CKAP4"
                     /gene_synonym="CLIMP-63; CLIMP63; ERGIC-63; p63"
                     /note="major polyA site"
ORIGIN      
        1 ctcctcggcc cggcctcctt ccccggccgc gcgtcctctc gccgctccga cgccagcagc
       61 gcccgcgtgc cgctcgccca gtcccggggg agcccctgca agtttcccgg gccgcgcgcc
      121 gcgctcgctc gcctcccagc ccgcggcccg agccgccgcc gcgcccgcca tgccctcggc
      181 caaacaaagg ggctccaagg gcggccacgg cgccgcgagc ccctcggaga agggtgccca
      241 cccgtcgggc ggcgcggatg acgtggcgaa gaagccgccg ccggcgccgc agcagccgcc
      301 gccgccgccc gcgccgcacc cgcagcagca cccgcagcag cacccgcaga accaggcgca
      361 cggcaagggc ggccaccgcg gcggcggcgg cggcggcggc aagtcctcct cctcctcctc
      421 cgcctccgcc gccgctgccg ccgccgccgc ctcgtcctcg gcgtcctgct cgcgcaggct
      481 cggcagggcg ctcaactttc tcttctacct cgccctggtg gcggcggccg ctttctcggg
      541 ctggtgcgtc caccacgtcc tggaggaggt ccagcaggtc cggcgcagcc accaggactt
      601 ctcccggcag agggaggagc tgggccaggg cttgcagggc gtcgagcaga aggtgcagtc
      661 tttgcaagcc acatttggaa cttttgagtc catcttgaga agctcccaac ataaacaaga
      721 cctcacagag aaagctgtga agcaagggga gagtgaggtc agccggatca gcgaagtgct
      781 gcagaaactc cagaatgaga ttctcaaaga cctctcggat gggatccatg tggtgaagga
      841 cgcccgggag cgggacttca cgtccctgga gaacacggtg gaggagcggc tgacggagct
      901 caccaaatcc atcaacgaca acatcgccat cttcacagaa gtccagaaga ggagccagaa
      961 ggagatcaat gacatgaagg caaaggttgc ctccctggaa gaatctgagg ggaacaagca
     1021 ggatttgaaa gccttaaagg aagctgtgaa ggagatacag acctcagcca agtccagaga
     1081 gtgggacatg gaggccctga gaagtaccct tcagactatg gagtctgaca tctacaccga
     1141 ggtccgcgag ctggtgagcc tcaagcagga gcagcaggct ttcaaggagg cggccgacac
     1201 ggagcggctc gccctgcagg ccctcacgga gaagcttctc aggtctgagg agtccgtctc
     1261 ccgcctcccg gaggagatcc ggagactgga ggaagagctc cgccagctga agtccgattc
     1321 ccacgggccg aaggaggacg gaggcttcag acactcggaa gcctttgagg cactccagca
     1381 aaagagtcag ggactggact ccaggctcca gcacgtggag gatggggtgc tctccatgca
     1441 ggtggcttct gcgcgccaga ccgagagcct ggagtccctc ctgtccaaga gccaggagca
     1501 cgagcagcgc ctggccgccc tgcaggggcg cctggaaggc ctcgggtcct cagaggcaga
     1561 ccaggatggc ctggccagca cggtgaggag cctgggcgag acccagctgg tgctctacgg
     1621 tgacgtggag gagctgaaga ggagtgtggg cgagctcccc agcaccgtgg aatcactcca
     1681 gaaggtgcag gagcaggtgc acacgctgct cagtcaggac caagcccagg ccgcccgtct
     1741 gcctcctcag gacttcctgg acagactttc ttctctagac aacctgaaag cctcagtcag
     1801 ccaagtggag gcggacttga aaatgctcag gactgctgtg gacagtttgg ttgcatactc
     1861 ggtcaaaata gaaaccaacg agaacaatct ggaatcagcc aagggtttac tagatgacct
     1921 gaggaatgat ctggataggt tgtttgtgaa agtggagaag attcacgaaa aggtctaaat
     1981 gaattgcgtg tgcagggcgc ggatttaaag tccaatttct catgaccaaa aaatgtgtgg
     2041 ttttttccca tgtgtcccct accccccaat ttcttgtccc ctcttaaaga gcagttgtca
     2101 ccacctgaac accaaggcat tgtattttca tgcccagtta acttatttac aatatttaag
     2161 ttctctgctt ctgcatttgg ttggtttcct gaagcgcagc ccctgtgaat aacaggtggc
     2221 ttttcatgga tgtctctagt cagagaaaaa tgataaaggc ttaaattgag gattaacaga
     2281 agcagattaa cctcagaaat cctgtctggc tggcagattt caagtaaaaa aaaaaaaaag
     2341 gtgggttggg gggacccttt tctttctagt tgtctttaag gaaaattaat tttacttttt
     2401 tttttgttct ggccgaaatt tttatgagat atctctcact tgtcttccac tttgaaccgg
     2461 ttaaagctca tagctgtcag ctctgaatga ggaggggaga agcccctggg tctttctttg
     2521 aaaggaatcc gctgcttgag ggctgcctcc ctcatggtgt gcgtgtcgtt tctcttcctg
     2581 acgcatcttg tgatatcaga ggtaactatg caaagcatcc aggcggttct gaatgtgaag
     2641 cactacaccc agcagagtcc cggtgccctc tgtccccact gccggcccat gttctctctc
     2701 cggaggtcac caaggaatgc acaggtttcg actaccagaa aggggagtcc ttgggttctt
     2761 tcaaaaaatt cgtgaggaga gctgtctaca gtggaatagg gggtctccct ggggaatgca
     2821 ggccaagtcc ttttatttta acatgatgtc catgaagagg tttgccgtct gggcagccct
     2881 gtcggcaagg agcgtgcata ctgcgtttgt gtaattgttt gctgtatctc ccttccctct
     2941 gagctgtatt gttctttaat ggctgtcttg cccttccaaa aaaaattgaa aaaaaaaaaa
     3001 aaaaaaaaac ccgaaaagta aaaaaaaaaa aaaaaagttt tgtttagttt actgtgaaat
     3061 gaactgtatg gcttatttac agtattttta attcttaata aacacttgag ctttgttatg
     3121 a
//
