LOCUS       NM_152515               4723 bp    mRNA    linear   PRI 02-APR-2024
DEFINITION  Homo sapiens cytoskeleton associated protein 2 like (CKAP2L),
            transcript variant 1, mRNA.
ACCESSION   NM_152515
VERSION     NM_152515.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4723)
  AUTHORS   Shi,Y., Xiong,X., Sun,Y., Geng,Z., Chen,X., Cui,X., Lv,J., Ge,L.,
            Jia,X. and Xu,J.
  TITLE     IGF2BP2 promotes ovarian cancer growth and metastasis by
            upregulating CKAP2L protein expression in an m6 A-dependent manner
  JOURNAL   FASEB J 37 (10), e23183 (2023)
   PUBMED   37665628
  REMARK    GeneRIF: IGF2BP2 promotes ovarian cancer growth and metastasis by
            upregulating CKAP2L protein expression in an m[6] A-dependent
            manner.
REFERENCE   2  (bases 1 to 4723)
  AUTHORS   Yi,B., Fu,Q., Zheng,Z., Zhang,M., Liu,D., Liang,Z., Xu,S. and
            Zhang,Z.
  TITLE     Pan-cancer analysis reveals the prognostic and immunotherapeutic
            value of cytoskeleton-associated protein 2-like
  JOURNAL   Sci Rep 13 (1), 8368 (2023)
   PUBMED   37225919
  REMARK    GeneRIF: Pan-cancer analysis reveals the prognostic and
            immunotherapeutic value of cytoskeleton-associated protein 2-like.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4723)
  AUTHORS   Li,Q., Yan,M., Wang,C., Wang,K. and Bao,G.
  TITLE     CKAP2L, a crucial target of miR-326, promotes prostate cancer
            progression
  JOURNAL   BMC Cancer 22 (1), 666 (2022)
   PUBMED   35715760
  REMARK    GeneRIF: CKAP2L, a crucial target of miR-326, promotes prostate
            cancer progression.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4723)
  AUTHORS   Patrick,R.J., Weimer,J., Davis-Keppen,L. and Landsverk,M.L.
  TITLE     Novel variants identified in CKAP2L in two siblings with Filippi
            syndrome
  JOURNAL   Cold Spring Harb Mol Case Stud 8 (2), a006130 (2022)
   PUBMED   34921061
  REMARK    GeneRIF: Novel variants identified in CKAP2L in two siblings with
            Filippi syndrome.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4723)
  AUTHORS   Chi,F., Chen,L., Jin,X., He,G., Liu,Z. and Han,S.
  TITLE     CKAP2L, transcriptionally inhibited by FOXP3, promotes breast
            carcinogenesis through the AKT/mTOR pathway
  JOURNAL   Exp Cell Res 412 (2), 113035 (2022)
   PUBMED   35065924
  REMARK    GeneRIF: CKAP2L, transcriptionally inhibited by FOXP3, promotes
            breast carcinogenesis through the AKT/mTOR pathway.
REFERENCE   6  (bases 1 to 4723)
  AUTHORS   Xu,N., Chen,S.H., Lin,T.T., Cai,H., Ke,Z.B., Dong,R.N., Huang,P.,
            Li,X.D., Chen,Y.H. and Zheng,Q.S.
  TITLE     Development and validation of hub genes for lymph node metastasis
            in patients with prostate cancer
  JOURNAL   J Cell Mol Med 24 (8), 4402-4414 (2020)
   PUBMED   32130760
  REMARK    GeneRIF: Development and validation of hub genes for lymph node
            metastasis in patients with prostate cancer.
REFERENCE   7  (bases 1 to 4723)
  AUTHORS   Hussain,M.S., Battaglia,A., Szczepanski,S., Kaygusuz,E.,
            Toliat,M.R., Sakakibara,S., Altmuller,J., Thiele,H., Nurnberg,G.,
            Moosa,S., Yigit,G., Beleggia,F., Tinschert,S., Clayton-Smith,J.,
            Vasudevan,P., Urquhart,J.E., Donnai,D., Fryer,A., Percin,F.,
            Brancati,F., Dobbie,A., Smigiel,R., Gillessen-Kaesbach,G.,
            Wollnik,B., Noegel,A.A., Newman,W.G. and Nurnberg,P.
  TITLE     Mutations in CKAP2L, the human homolog of the mouse Radmis gene,
            cause Filippi syndrome
  JOURNAL   Am J Hum Genet 95 (5), 622-632 (2014)
   PUBMED   25439729
  REMARK    GeneRIF: Loss-of-function mutations in CKAP2L are a major cause of
            Filippi syndrome.
REFERENCE   8  (bases 1 to 4723)
  AUTHORS   Yumoto,T., Nakadate,K., Nakamura,Y., Sugitani,Y.,
            Sugitani-Yoshida,R., Ueda,S. and Sakakibara,S.
  TITLE     Radmis, a novel mitotic spindle protein that functions in cell
            division of neural progenitors
  JOURNAL   PLoS One 8 (11), e79895 (2013)
   PUBMED   24260314
  REMARK    GeneRIF: Radmis, the mouse ortholog of human CKAP2L, is important
            to mitotic spindle formation and cell-cycle progression of neural
            stem/neural progenitor cells in mouse. It is abundantly expressed
            in stem cells, but is down-regulated during differentiation.
            Publication Status: Online-Only
REFERENCE   9  (bases 1 to 4723)
  AUTHORS   Jakobsen,L., Vanselow,K., Skogs,M., Toyoda,Y., Lundberg,E.,
            Poser,I., Falkenby,L.G., Bennetzen,M., Westendorf,J., Nigg,E.A.,
            Uhlen,M., Hyman,A.A. and Andersen,J.S.
  TITLE     Novel asymmetrically localizing components of human centrosomes
            identified by complementary proteomics methods
  JOURNAL   EMBO J 30 (8), 1520-1535 (2011)
   PUBMED   21399614
REFERENCE   10 (bases 1 to 4723)
  AUTHORS   Dick,D.M., Meyers,J., Aliev,F., Nurnberger,J. Jr., Kramer,J.,
            Kuperman,S., Porjesz,B., Tischfield,J., Edenberg,H.J., Foroud,T.,
            Schuckit,M., Goate,A., Hesselbrock,V. and Bierut,L.
  TITLE     Evidence for genes on chromosome 2 contributing to alcohol
            dependence with conduct disorder and suicide attempts
  JOURNAL   Am J Med Genet B Neuropsychiatr Genet 153B (6), 1179-1188 (2010)
   PUBMED   20468071
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA506407.1, AL832036.1,
            BF742811.1, AK292530.1, AK097948.1 and AC079922.5.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_152515.4.
            
            Summary: The protein encoded by this gene is thought to be a
            mitotic spindle protein important to neural stem or progenitor
            cells. Mutations in this gene have been associated with spindle
            organization defects, including mitotic spindle defects, lagging
            chromosomes, and chromatin bridges. There is evidence that
            mutations in this gene are associated with Filippi syndrome,
            characterized by growth defects, microcephaly, intellectual
            disability, facial feature defects, and syndactyly. There is a
            pseudogene of this gene on chromosome 20. Alternative splicing
            results in multiple transcript variants. [provided by RefSeq, Jan
            2015].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AL832036.1, SRR1803616.91085.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000302450.11/ ENSP00000305204.6
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-499               DA506407.1         69-567
            500-1133            AL832036.1         519-1152
            1134-1134           BF742811.1         247-247
            1135-2195           AL832036.1         1154-2214
            2196-2863           AK292530.1         2215-2882
            2864-3205           AK097948.1         2115-2456
            3206-4119           AL832036.1         3225-4138
            4120-4120           AC079922.5         119344-119344       c
            4121-4398           AL832036.1         4140-4417
            4399-4399           AC079922.5         119065-119065       c
            4400-4723           AL832036.1         4419-4742
FEATURES             Location/Qualifiers
     source          1..4723
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q14.1"
     gene            1..4723
                     /gene="CKAP2L"
                     /note="cytoskeleton associated protein 2 like"
                     /db_xref="GeneID:150468"
                     /db_xref="HGNC:HGNC:26877"
                     /db_xref="MIM:616174"
     exon            1..48
                     /gene="CKAP2L"
                     /inference="alignment:Splign:2.1.0"
     CDS             12..2249
                     /gene="CKAP2L"
                     /note="isoform 1 is encoded by transcript variant 1;
                     radmis; radial fiber and mitotic spindle protein"
                     /codon_start=1
                     /product="cytoskeleton-associated protein 2-like isoform
                     1"
                     /protein_id="NP_689728.3"
                     /db_xref="CCDS:CCDS2100.1"
                     /db_xref="GeneID:150468"
                     /db_xref="HGNC:HGNC:26877"
                     /db_xref="MIM:616174"
                     /translation="MVGPGPTAAAAVEERQRKLQEYLAAKGKLKSQNTKPYLKSKNNC
                     QNQPPSKSTIRPKNDVTNHVVLPVKPKRSISIKLQPRPPNTAGSQKPKLEPPKLLGKR
                     LTSECVSSNPYSKPSSKSFQQCEAGSSTTGELSRKPVGSLNIEQLKTTKQQLTDQGNG
                     KCIDFMNNIHVENESLDNFLKETNKENLLDILTEPERKPDPKLYTRSKPKTDSYNQTK
                     NSLVPKQALGKSSVNSAVLKDRVNKQFVGETQSRTFPVKSQQLSRGADLARPGVKPSR
                     TVPSHFIRTLSKVQSSKKPVVKNIKDIKVNRSQYERPNETKIRSYPVTEQRVKHTKPR
                     TYPSLLQGEYNNRHPNIKQDQKSSQVCIPQTSCVLQKSKAISQRPNLTVGRFNSAIPS
                     TPSIRPNGTSGNKHNNNGFQQKAQTLDSKLKKAVPQNHFLNKTAPKTQADVTTVNGTQ
                     TNPNIKKKATAEDRRKQLEEWQKSKGKTYKRPPMELKTKRKVIKEMNISFWKSIEKEE
                     EEKKAQLELSSKINNTLTECLNLIEGGVPSNEILNILSSIPEAEKFAKFWICKAKLLA
                     SKGTFDVIGLYEEAIKNGATPIQELRKVVLNILQDSNRTTEGITSDSLVAETSITSVE
                     ELAKKMESVKSCLSPKEREQVTATPRIAKAEQHNYPGIKLQIGPIPRINGMPEVQDMK
                     FITPVRRSSRIERAVSRYPEMLQEHDLVVASLDELLEVEETKCFIFRRNEALPVTLGF
                     QTPES"
     exon            49..115
                     /gene="CKAP2L"
                     /inference="alignment:Splign:2.1.0"
     exon            116..167
                     /gene="CKAP2L"
                     /inference="alignment:Splign:2.1.0"
     exon            168..1405
                     /gene="CKAP2L"
                     /inference="alignment:Splign:2.1.0"
     exon            1406..1613
                     /gene="CKAP2L"
                     /inference="alignment:Splign:2.1.0"
     exon            1614..1769
                     /gene="CKAP2L"
                     /inference="alignment:Splign:2.1.0"
     exon            1770..1833
                     /gene="CKAP2L"
                     /inference="alignment:Splign:2.1.0"
     exon            1834..2023
                     /gene="CKAP2L"
                     /inference="alignment:Splign:2.1.0"
     exon            2024..4723
                     /gene="CKAP2L"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3184..3189
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CKAP2L"
                     /note="hexamer: ATTAAA"
     polyA_site      3205
                     /gene="CKAP2L"
     regulatory      4705..4710
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CKAP2L"
                     /note="hexamer: AATAAA"
     polyA_site      4723
                     /gene="CKAP2L"
                     /note="major polyA site"
ORIGIN      
        1 actgaagagt catggtgggg cccgggccta ccgctgctgc cgctgtcgaa gagcggcaga
       61 gaaagcttca ggagtacctt gcagccaagg gaaaactgaa gagccaaaac accaagcctt
      121 atctaaaatc caagaataat tgccagaatc aaccaccttc taaatctact attagaccca
      181 aaaatgatgt taccaaccat gttgttttgc ctgtcaaacc taaaaggtcc atcagcatta
      241 aactccagcc cagaccacct aatactgcag ggtcccagaa gccgaagttg gagccaccaa
      301 aacttctggg caaaaggctg acttcagaat gtgtttcttc taacccatac tctaagcctt
      361 ctagcaagag ttttcaacag tgtgaagctg gatcgtccac aacaggagaa ctgtcaagaa
      421 aacctgtggg gtcacttaat atagagcaat tgaaaactac aaagcagcag ttaacagatc
      481 aaggaaatgg taaatgtata gactttatga ataatatcca tgttgaaaac gaatctttgg
      541 ataactttct aaaagaaaca aacaaagaga acttgctcga tatcttaaca gaacctgaga
      601 ggaagccaga tcctaaatta tataccagaa gtaagccaaa gactgactct tataatcaaa
      661 ccaagaacag tttagttcct aaacaagcct tgggcaaaag ttcagttaat agtgctgttc
      721 tgaaagatag ggttaataaa caatttgttg gagaaacaca aagcaggact ttcccagtaa
      781 aatcacagca actctctaga ggagcagatc ttgcaagacc aggagtaaaa ccctcaagga
      841 cggttccctc tcactttatt cggaccctta gtaaagttca gtcatcaaag aaaccagtag
      901 tcaagaacat caaagatata aaggttaata ggagtcaata tgaaagacca aatgaaacta
      961 agatacggtc ataccctgtt actgaacaga gagtgaagca caccaaaccc agaacatacc
     1021 ccagtttgct tcagggtgaa tataacaaca gacatccaaa catcaagcaa gatcagaagt
     1081 ccagccaagt ttgtatacct cagacatcat gtgtactgca aaagtcaaaa gccataagcc
     1141 agaggcctaa tttgacagtt ggcagattta attcagccat tccaagcacc cctagcataa
     1201 gaccaaatgg aaccagtggt aataaacata acaataatgg ctttcagcaa aaagcacaga
     1261 ctttggactc caagttgaaa aaggctgttc cccagaacca ttttctgaac aagacagctc
     1321 ccaaaactca agctgatgtc acaaccgtaa atgggaccca aacaaaccca aatattaaaa
     1381 agaaggcaac agcagaggat cgaaggaaac aactagaaga atggcagaaa tctaagggaa
     1441 aaacctataa acggcctcct atggaactta aaacaaaaag aaaagtaata aaggaaatga
     1501 atatttcatt ctggaagagc attgaaaaag aagaggaaga aaagaaagca caactcgaac
     1561 tgtccagtaa aattaacaac actctgacag aatgtctgaa cctcatcgaa gggggtgtac
     1621 cttctaatga aatacttaac atattgtcca gcattcctga agctgaaaaa tttgctaaat
     1681 tctggatctg caaagcaaag ttgttggcaa gtaaaggcac ctttgatgtt attgggctat
     1741 atgaagaggc cattaaaaat ggggcaacac caatacaaga gttgcggaaa gttgttctta
     1801 atatcttgca agactcaaac agaaccacag aagggattac ttctgactct ttagttgctg
     1861 aaactagtat aacatcagtg gaagagctgg ccaagaagat ggaatctgtg aagtcttgtc
     1921 tttctccaaa agagagggaa caagtcacgg cgacaccccg aatagccaag gcagaacagc
     1981 ataattatcc tggtatcaaa ttacagattg gtccaatccc tagaataaat gggatgccgg
     2041 aagtgcaaga catgaaattt atcactcctg tacggcgttc gtcgaggatt gagcgagcag
     2101 tgtcccgcta cccagaaatg ctgcaggaac acgatttagt agtggcttct cttgatgaac
     2161 tgttagaagt ggaagaaaca aaatgtttta tattccgtag aaatgaggcg ctgcctgtaa
     2221 cattggggtt tcaaacccct gaatcataat ttcttgatgc tttttttaaa aaaggtgttt
     2281 cagaaccaac ataagacaag aaatattctt gtccaagtga cctggaaaaa aagagaggca
     2341 tcatggatgc agattatgga cgctcaggac ttaagtaatt cactgggctt actctcaaat
     2401 tttctctccc catgggaaaa tctttgcttt atgtgtttaa gaccagtttg ccagttttac
     2461 agtatacata aatttcaaac ttttgaatat ttaccctaca actatgataa atatttatac
     2521 tttattgatc tactttaatc ccaacatagt tttttatatc agaaggttgg tcccacaata
     2581 taatgggact tttctttctg catccacctt agcagagggc aagttctttc atcgtggaag
     2641 atctgaactt tgacgctagc ttgacaggct gaatggacct tgacagatgg ccaagtcaaa
     2701 acccctcatt ttacagatga gtttatgcta gctgtgcctt tgctcagaga tcataaattt
     2761 atgtgtgatc tttgactttc atcacagtat ccttgtaaat gagagagaat tcttgttttt
     2821 tctatgccag gtctcctcag atgaaacaca atcctgaatt ggcatggtcg tctagctttt
     2881 atattcaaac ctagttttgc atttgttact cagaaacact tcaagtaaca aaaggggggc
     2941 aggggagagg ggtggggagt ggacacttgc ttcttcagtt ctttgctcag ctagggtata
     3001 gctggcctga gaaggcagtg caagccccag agactgttgt aggttgcttt ttcttcctcc
     3061 tgggcaacta tttgtggaaa ggttttacct tagtcattat gtaaatataa ttgtgtagaa
     3121 aaacctagtc aacacatttt aaattttagc tttcttaata tttaagtatt atcttaaaac
     3181 aacattaaat ttctattagt tcctgcttgt ttatcttatt tattatagaa aaccatgata
     3241 atggctttgc atagagaatt aggagtttgt ctaaggatat accagttggc tgtcctaaga
     3301 aacaaggagc attcattacc aaggagaatg gactttgatc aaaggtctat cagcctcatc
     3361 caggaactag atttttttaa gggaagcaga tctatttctg aaagtcagat ttataataaa
     3421 gctcaaaaaa ctgagctata aacctgtaat accagcagac tttcaataag agactcttac
     3481 aactcaattg tggaaaaact aataattaaa aatgggcaaa ggacctgagc agacatttct
     3541 ccaaagaaga cataagaaat ggccagtagg tatagatatg aaaaggtgtt cagcatcatt
     3601 aatcataaga ataatgtaaa ttaaaaccac tgtgagctat cacctcacat ctataagaat
     3661 ggctattaac aagacatgag ataaatgttg atgagattgt ggagaaaaga gaaccctagt
     3721 acactgtttg tgggcgtgta gactggggca gccgttatgg aaaacggtat ggaggctcct
     3781 aaagaaatta aaaatagaac tgttatctga ccctcttctg agtaagtatg tacccaaaga
     3841 agatgaaatc accagctggg cgcagtgact cacacctgta atcccagcac tttggagtgg
     3901 gtgaatcacc tgaggtcagg agttcaagac cagcttgacc aacatggtga aaccccgtct
     3961 ctactaaaaa tacaaaaagt aggcgggcat ggtgacgggc acctgtaatc ccagctactt
     4021 gggaggctga ggcagaagaa tcacttgaac tcgggaggtg gaggttgcag tgagccaaaa
     4081 ttgcgtcact gcactccagc ctgggttaca gagcaagact ccatctcaaa aaaaaaaaag
     4141 atgaaatcat cacctcataa agatatctgc actcacatgt ttgtggcagt gttattctca
     4201 atagccaaga tgtggaaaca acctaaatgc ccatcaatgg acaaataaag aaaatacggc
     4261 atatgcatgc cgtggaatag tattcatcct tggaaaagag ggagttcttg ccatttgcca
     4321 caacatagat ggacctggag aacattatgc taagtgaaat aagccagacc caaggaaaaa
     4381 tactgcatga tctcacatat ggaatattta attttttaag aaagagctca agtacacaga
     4441 gaaagtgctt accacagatt ggggaagagg aaatggggag atgcaggcca aggatacaaa
     4501 atagcagata aaatgaacaa gtctagagat agggctaaag ttaatacaat tgtattaggg
     4561 atttttgtta aataagtaga ttttagctgc tattatcaca aaaaaactga gatgataatg
     4621 ttaatctgct tcactatagc agccatttta ttatctatat gtatcccata acatcatgtt
     4681 gtaaatctta aatataccta ataaaataaa attgtcacca aaa
//
