LOCUS       NM_018204               3614 bp    mRNA    linear   PRI 02-APR-2024
DEFINITION  Homo sapiens cytoskeleton associated protein 2 (CKAP2), transcript
            variant 1, mRNA.
ACCESSION   NM_018204
VERSION     NM_018204.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3614)
  AUTHORS   Zhang,S., Li,N., Yang,Y., Li,Y., Lin,C., Fu,G. and Cai,J.
  TITLE     CKAP2 overexpression correlates with worse overall survival in
            patients with lung adenocarcinoma
  JOURNAL   Medicine (Baltimore) 101 (30), e29796 (2022)
   PUBMED   35905213
  REMARK    GeneRIF: CKAP2 overexpression correlates with worse overall
            survival in patients with lung adenocarcinoma.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3614)
  AUTHORS   Ma,H.N., Chen,H.J., Liu,J.Q. and Li,W.T.
  TITLE     Long non-coding RNA DLEU1 promotes malignancy of breast cancer by
            acting as an indispensable coactivator for HIF-1alpha-induced
            transcription of CKAP2
  JOURNAL   Cell Death Dis 13 (7), 625 (2022)
   PUBMED   35853854
  REMARK    GeneRIF: Long non-coding RNA DLEU1 promotes malignancy of breast
            cancer by acting as an indispensable coactivator for
            HIF-1alpha-induced transcription of CKAP2.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3614)
  AUTHORS   Feng,Y., Wei,G., Zhang,L., Zhou,H., Wang,W., Guo,P., Cheng,C.,
            Ji,L., Cai,Q., Feng,Y. and Tu,H.
  TITLE     LncRNA DARS-AS1 aggravates the growth and metastasis of
            hepatocellular carcinoma via regulating the
            miR-3200-5p-Cytoskeleton associated protein 2 (CKAP2) axis
  JOURNAL   Bioengineered 12 (1), 8217-8232 (2021)
   PUBMED   34596006
  REMARK    GeneRIF: LncRNA DARS-AS1 aggravates the growth and metastasis of
            hepatocellular carcinoma via regulating the
            miR-3200-5p-Cytoskeleton associated protein 2 (CKAP2) axis.
REFERENCE   4  (bases 1 to 3614)
  AUTHORS   Chen,X., Xie,J., Cui,Y., Zhang,L., Yu,H., Chen,J., He,M., Liu,S.,
            Huang,T., Chen,L., Fang,D., Zhu,Y., Zhang,S. and Meng,Q.
  TITLE     Cytoskeleton-associated protein 2 (CKAP2) is regulated by vascular
            endothelial growth factor and p53 in retinal capillary endothelial
            cells under high-glucose conditions
  JOURNAL   Mol Cell Endocrinol 535, 111378 (2021)
   PUBMED   34216644
  REMARK    GeneRIF: Cytoskeleton-associated protein 2 (CKAP2) is regulated by
            vascular endothelial growth factor and p53 in retinal capillary
            endothelial cells under high-glucose conditions.
REFERENCE   5  (bases 1 to 3614)
  AUTHORS   Bae,C.D., Sung,Y.S., Jeon,S.M., Suh,Y., Yang,H.K., Kim,Y.I.,
            Park,K.H., Choi,J., Ahn,G. and Park,J.
  TITLE     Up-regulation of cytoskeletal-associated protein 2 in primary human
            gastric adenocarcinomas
  JOURNAL   J Cancer Res Clin Oncol 129 (11), 621-630 (2003)
   PUBMED   12942315
REFERENCE   6  (bases 1 to 3614)
  AUTHORS   Rakhmanaliev,E.R., Klimov,E.A., Kompaniitsev,A.A. and Sulimova,G.E.
  TITLE     [The structure of the human oncogenesis-associated CKAP2 (LB1)
            gene]
  JOURNAL   Mol Biol (Mosk) 36 (6), 985-989 (2002)
   PUBMED   12500535
  REMARK    GeneRIF: CKAP2 comprises nine exons ranging 70-1442 bp and is about
            22 kb in size (regulatory regions included). The CKAP2 promoter
            contains CCAAT (-39...-33) rather than the canonical TATA box, and
            harbors nine binding sites for six transcription factors
REFERENCE   7  (bases 1 to 3614)
  AUTHORS   Eichmuller,S., Usener,D., Dummer,R., Stein,A., Thiel,D. and
            Schadendorf,D.
  TITLE     Serological detection of cutaneous T-cell lymphoma-associated
            antigens
  JOURNAL   Proc Natl Acad Sci U S A 98 (2), 629-634 (2001)
   PUBMED   11149944
REFERENCE   8  (bases 1 to 3614)
  AUTHORS   Udina,I.G., Baranova,A.V., Kompaniitsev,A.A. and Sulimova,G.E.
  TITLE     [Evolutionarily-conserved gene CKAP2,located in region 13q14.3 of
            the human genome, is frequently rearranged in various tumors]
  JOURNAL   Genetika 37 (1), 120-123 (2001)
   PUBMED   11234418
REFERENCE   9  (bases 1 to 3614)
  AUTHORS   Maouche-Chretien,L., Deleu,N., Badoual,C., Fraissignes,P.,
            Berger,R., Gaulard,P., Romeo,P.H. and Leroy-Viard,K.
  TITLE     Identification of a novel cDNA, encoding a cytoskeletal associated
            protein, differentially expressed in diffuse large B cell lymphomas
  JOURNAL   Oncogene 17 (10), 1245-1251 (1998)
   PUBMED   9771967
REFERENCE   10 (bases 1 to 3614)
  AUTHORS   Adams,M.D., Kerlavage,A.R., Fleischmann,R.D., Fuldner,R.A.,
            Bult,C.J., Lee,N.H., Kirkness,E.F., Weinstock,K.G., Gocayne,J.D.,
            White,O. et al.
  TITLE     Initial assessment of human gene diversity and expression patterns
            based upon 83 million nucleotides of cDNA sequence
  JOURNAL   Nature 377 (6547 Suppl), 3-174 (1995)
   PUBMED   7566098
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK022982.1, AY062261.1,
            AI499799.1 and EC439890.1.
            
            On Nov 23, 2018 this sequence version replaced NM_018204.4.
            
            Summary: This gene encodes a cytoskeleton-associated protein that
            stabalizes microtubules and plays a role in the regulation of cell
            division. The encoded protein is itself regulated through
            phosphorylation at multiple serine and threonine residues. There is
            a pseudogene of this gene on chromosome 14. Alternative splicing
            results in multiple transcript variations. [provided by RefSeq, Nov
            2013].
            
            Transcript Variant: This variant (1) encodes isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803611.171896.1, AK022982.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000258607.10/ ENSP00000258607.5
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-900               AK022982.1         18-917
            901-3330            AY062261.1         940-3369
            3331-3613           AI499799.1         1-283               c
            3614-3614           EC439890.1         7-7                 c
FEATURES             Location/Qualifiers
     source          1..3614
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="13"
                     /map="13q14.3"
     gene            1..3614
                     /gene="CKAP2"
                     /gene_synonym="LB1; se20-10; TMAP"
                     /note="cytoskeleton associated protein 2"
                     /db_xref="GeneID:26586"
                     /db_xref="HGNC:HGNC:1990"
                     /db_xref="MIM:611569"
     exon            1..149
                     /gene="CKAP2"
                     /gene_synonym="LB1; se20-10; TMAP"
                     /inference="alignment:Splign:2.1.0"
     CDS             80..2128
                     /gene="CKAP2"
                     /gene_synonym="LB1; se20-10; TMAP"
                     /note="isoform 1 is encoded by transcript variant 1;
                     tumor-associated microtubule-associated protein; CTCL
                     tumor antigen se20-10; tumor- and microtubule-associated
                     protein"
                     /codon_start=1
                     /product="cytoskeleton-associated protein 2 isoform 1"
                     /protein_id="NP_060674.3"
                     /db_xref="CCDS:CCDS9435.1"
                     /db_xref="GeneID:26586"
                     /db_xref="HGNC:HGNC:1990"
                     /db_xref="MIM:611569"
                     /translation="MSTPAVPQDLQLPPSQRAQSAFKEQRRQKLKEHLLRRKTLFAYK
                     QENEMLSSRDQRVVTSEDQVQEGTKVLKLKTKMADKENMKRPAESKNNTVVGKHCIPL
                     KPSNELTNSTVVIDTHKPKDSNQTPHLLLTEDDPQSQHMTLSQAFHLKNNSKKKQMTT
                     EKQKQDANMPKKPVLGSYRGQIVQSKINSFRKPLQVKDESSAATKKLSATIPKATKPQ
                     PVNTSSVTVKSNRSSNMTATTKFVSTTSQNTQLVRPPIRSHHSNTRDTVKQGISRTSA
                     NVTIRKGPHEKELLQSKTALSSVKTSSSQGIIRNKTLSRSIASEVIARPASLSNDKLM
                     EKSEPVDQRRHTAGKAIVDSRSAQPKETSEERKARLSEWKAGKGRVLKRPPNSVVTQH
                     EPAGQNEKPVGSFWTTMAEEDEQRLFTEKVNNTFSECLNLINEGCPKEDILVTLNDLI
                     KNIPDAKKLVKYWICLALIEPITSPIENIIAIYEKAILAGAQPIEEMRHTIVDILTMK
                     SQEKANLGENMEKSCASKEEVKEVSIEDTGVDVDPEKLEMESKLHRNLLFQDCEKEQD
                     NKTKDPTHDVKTPNTETRTSCLIKYNVSTTPYLQSVKKKVQFDGTNSAFKELKFLTPV
                     RRSRRLQEKTSKLPDMLKDHYPCVSSLEQLTELGRETDAFVCRPNAALCRVYYEADTT
                     "
     exon            150..234
                     /gene="CKAP2"
                     /gene_synonym="LB1; se20-10; TMAP"
                     /inference="alignment:Splign:2.1.0"
     exon            235..310
                     /gene="CKAP2"
                     /gene_synonym="LB1; se20-10; TMAP"
                     /inference="alignment:Splign:2.1.0"
     exon            311..1179
                     /gene="CKAP2"
                     /gene_synonym="LB1; se20-10; TMAP"
                     /inference="alignment:Splign:2.1.0"
     exon            1180..1384
                     /gene="CKAP2"
                     /gene_synonym="LB1; se20-10; TMAP"
                     /inference="alignment:Splign:2.1.0"
     exon            1385..1555
                     /gene="CKAP2"
                     /gene_synonym="LB1; se20-10; TMAP"
                     /inference="alignment:Splign:2.1.0"
     exon            1556..1625
                     /gene="CKAP2"
                     /gene_synonym="LB1; se20-10; TMAP"
                     /inference="alignment:Splign:2.1.0"
     exon            1626..1881
                     /gene="CKAP2"
                     /gene_synonym="LB1; se20-10; TMAP"
                     /inference="alignment:Splign:2.1.0"
     exon            1882..3614
                     /gene="CKAP2"
                     /gene_synonym="LB1; se20-10; TMAP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2133..2138
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CKAP2"
                     /gene_synonym="LB1; se20-10; TMAP"
                     /note="hexamer: AATAAA"
     polyA_site      2152
                     /gene="CKAP2"
                     /gene_synonym="LB1; se20-10; TMAP"
                     /note="major polyA site"
     regulatory      3317..3322
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CKAP2"
                     /gene_synonym="LB1; se20-10; TMAP"
                     /note="hexamer: ATTAAA"
     polyA_site      3337
                     /gene="CKAP2"
                     /gene_synonym="LB1; se20-10; TMAP"
                     /note="major polyA site"
     regulatory      3592..3597
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CKAP2"
                     /gene_synonym="LB1; se20-10; TMAP"
                     /note="hexamer: AATAAA"
     polyA_site      3614
                     /gene="CKAP2"
                     /gene_synonym="LB1; se20-10; TMAP"
                     /note="major polyA site"
ORIGIN      
        1 gcagactgcg gcggcggtgg tctgaggaag ttctatcttg gcgctaaagc ggagacgcat
       61 cccccgaccc gaggctacga tgagcacacc ggccgtgccc caggacctgc agctgccccc
      121 gagtcagagg gcgcagtccg cattcaaaga gcaaagaaga caaaaactca aggaacatct
      181 gttgagaaga aaaacgcttt ttgcatacaa gcaggaaaat gagatgttat ccagtagaga
      241 tcagagagtt gtgacatctg aggaccaagt tcaagaaggg actaaagtgc tgaaacttaa
      301 aacaaaaatg gctgataaag aaaacatgaa gagacctgca gagagcaaaa ataatacagt
      361 ggtggggaaa cattgtattc ctttaaaacc ttcaaatgaa ctaaccaatt caactgtagt
      421 aattgacaca cataaaccta aggatagtaa tcaaactccg catttgttac taactgaaga
      481 tgatccccaa agtcaacata tgacattaag ccaggcattt caccttaaaa acaatagtaa
      541 aaagaaacaa atgactacag aaaaacaaaa gcaagatgct aacatgccca agaaacctgt
      601 gcttggatct tatcgtggcc agattgttca gtctaagatt aattcattta gaaaacctct
      661 acaagtcaaa gatgagagtt ctgcagcaac aaagaaactt tcagccacta tacctaaagc
      721 cacaaaacct cagcctgtaa acaccagcag tgtaacagtg aaaagtaata gatcctccaa
      781 tatgactgcc actactaaat ttgtgagcac tacatctcag aacacacaac ttgtgcgacc
      841 tcctattaga agtcatcaca gtaatacccg ggacactgtg aaacaaggca tcagtagaac
      901 ttctgccaat gttacaatcc ggaaagggcc tcatgaaaaa gaactattac aatcaaaaac
      961 agctttatct agtgtcaaaa ccagttcttc tcaaggtata ataagaaata agactctatc
     1021 aagatccata gcatctgaag ttatagccag gcctgcttca ttgtctaatg ataaactgat
     1081 ggaaaagtca gagcccgttg accagcgaag acatactgca ggaaaagcaa ttgttgatag
     1141 tagatcagct cagcccaaag aaacctcgga agagagaaaa gctcgtctga gtgagtggaa
     1201 agctggcaaa ggaagagtgc taaaaaggcc ccctaattca gtagttactc agcatgagcc
     1261 tgcaggacaa aatgaaaaac cagttgggtc tttttggact accatggcag aagaagatga
     1321 acaaagatta tttactgaaa aagtaaacaa cacattttct gaatgcctga acttgattaa
     1381 tgagggatgt ccaaaagaag atatactggt cacactgaat gacctgatta aaaatattcc
     1441 agatgccaaa aagcttgtta agtattggat atgtcttgca cttattgaac caatcacaag
     1501 tcctattgaa aatattattg caatctatga gaaagccatt ctggcagggg ctcagcctat
     1561 tgaagagatg cgacacacga ttgtagatat tctaacaatg aagagtcaag aaaaagctaa
     1621 tttaggagaa aatatggaga agtcttgtgc aagcaaggaa gaagtcaaag aagtcagtat
     1681 tgaagataca ggtgttgatg tagatccaga aaaactggaa atggagagta aacttcatag
     1741 aaatttgcta tttcaagatt gtgaaaaaga gcaagacaac aaaacaaaag atccaaccca
     1801 tgatgttaaa acccccaata cagaaacgag gacaagttgc ttaattaaat ataatgtgtc
     1861 tactacgcca tacttgcaaa gtgtgaaaaa aaaggtgcag tttgatggaa caaattccgc
     1921 atttaaagag ctgaagtttt taacaccagt gagacgttct cgacgtcttc aagagaaaac
     1981 ttctaaattg ccagatatgt taaaagatca ttatccttgt gtgtcttcat tggaacagct
     2041 aacggagttg ggaagagaaa ctgatgcttt tgtatgccgc cctaatgcag cactgtgccg
     2101 ggtgtactat gaggctgata caacataaga gaaataaagc tctgttaggg aatggggttt
     2161 ttattatttg tggggtgttt tgttttgagt agctttatat tgctcttagg tctggagttg
     2221 gccatgtacc tatgtatcct aagcattcac ggcagtgagc tcctttacta acattcatgt
     2281 tatggcaaga gttgtcctct acattggaaa gctaatccta ccttgtcagt ttcaaccaac
     2341 tgagtttttt ctttaagaaa ggtaaatttt gtcagctagt ttactatgtt ccttgaatat
     2401 aaacaggtta taatactacc ctgttcactt tactaaatat aagtacagta atgatgcata
     2461 attagaaaat gaggtattct aggtaaaatg tatgtttgcc ttgacatgtt tttaaaagtt
     2521 atgatgtacc tccctgcctt taaacagaat acttttttct tttttttggc ctttctcaga
     2581 ttagtcaaaa attctataga atgactcact tcgaatacta agacacagga ggtttagcct
     2641 gctttcttac caaattcatg ttacccagac ttgtgttctc ttgcgtccct tggactgcct
     2701 gttgattgat ggaaagtgtc tgcactgaca cttttcgtca gtagtctgta gtttcgtggc
     2761 ctcttttgat tataactggg gtcaccaaga aggtttactt aattaaatac cgcatttcta
     2821 agagaagata ctttgtgtaa gaaaagatgc cacatttagt ggtttaactt ttgtaacttc
     2881 acttgatagt ttttaagcaa ttagaatgga gttagggaaa gaacatatca tactgaacaa
     2941 atgtcattct agtttagata gcatttctaa gataactgat actaatactt gttttcttcc
     3001 ctataacata aaaaacttca ctgttaagtc atgtcccttg aaacatgata gttacataca
     3061 cagttttctc tccacacata aataacacca ctaaagttgt tttgtaaggt tccaaactaa
     3121 tatggcatat atcaactcta cagtttcaaa taaatgactt tttaattgta aaagattagt
     3181 tgaaaaactg tatgaatgtg aagatcacat gcttagtcat ttttatgttc attccacttt
     3241 gtatatcttt tctatttatt gacttctcat gttctagaga gtaggacttt tattccgtgt
     3301 acctgatata tatacaatta aaatatctgt gtaattatgt tttaatgaag tttatttcaa
     3361 cagatatttt atgaaatgca gtttaaccac agtgaatgaa ttttagcttt gcttttgaaa
     3421 tacattgtaa gatttgactt gaggtttttg acacttaagt acatgcttaa tgatatattt
     3481 tcaaaagtca tcagtctact tacataaact ttacctaatt tttttcactt taacaaaaac
     3541 ataactatgc ccttgttttt tcatactttg acccaaaata tgccattttg aaataaaatt
     3601 taagctttga taca
//
