LOCUS       NM_001354640            2656 bp    mRNA    linear   PRI 09-FEB-2025
DEFINITION  Homo sapiens ciliated left-right organizer metallopeptidase
            (CIROP), transcript variant 1, mRNA.
ACCESSION   NM_001354640
VERSION     NM_001354640.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Szenker-Ravi,E., Ott,T., Khatoo,M., Moreau de Bellaing,A.,
            Goh,W.X., Chong,Y.L., Beckers,A., Kannesan,D., Louvel,G.,
            Anujan,P., Ravi,V., Bonnard,C., Moutton,S., Schoen,P., Fradin,M.,
            Colin,E., Megarbane,A., Daou,L., Chehab,G., Di Filippo,S.,
            Rooryck,C., Deleuze,J.F., Boland,A., Arribard,N., Eker,R.,
            Tohari,S., Ng,A.Y., Rio,M., Lim,C.T., Eisenhaber,B., Eisenhaber,F.,
            Venkatesh,B., Amiel,J., Crollius,H.R., Gordon,C.T., Gossler,A.,
            Roy,S., Attie-Bitach,T., Blum,M., Bouvagnet,P. and Reversade,B.
  TITLE     Discovery of a genetic module essential for assigning left-right
            asymmetry in humans and ancestral vertebrates
  JOURNAL   Nat Genet 54 (1), 62-72 (2022)
   PUBMED   34903892
  REMARK    GeneRIF: Discovery of a genetic module essential for assigning
            left-right asymmetry in humans and ancestral vertebrates.
            Erratum:[Nat Genet. 2022 Jun;54(6):906. doi:
            10.1038/s41588-022-01053-8.. PMID: 35304595]
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL117258.4.
            
            On Nov 23, 2018 this sequence version replaced NM_001354640.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660803.241722.1 [ECO:0000332]
            RNAseq introns              :: partial sample support SAMEA1968540,
                                           SAMEA2144120 [ECO:0000350]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000637218.2/ ENSP00000489869.1
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-391               AL117258.4         140092-140482
            392-476             AL117258.4         140612-140696
            477-528             AL117258.4         140787-140838
            529-672             AL117258.4         141263-141406
            673-750             AL117258.4         141533-141610
            751-846             AL117258.4         141699-141794
            847-972             AL117258.4         142030-142155
            973-1057            AL117258.4         142368-142452
            1058-1203           AL117258.4         142592-142737
            1204-1378           AL117258.4         142815-142989
            1379-1448           AL117258.4         143170-143239
            1449-1545           AL117258.4         143316-143412
            1546-1818           AL117258.4         143517-143789
            1819-2048           AL117258.4         144002-144231
            2049-2228           AL117258.4         144400-144579
            2229-2656           AL117258.4         145592-146019
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="14"
                     /map="14q11.2"
     gene            1..2656
                     /gene="CIROP"
                     /gene_synonym="HTX12; LMLN2"
                     /note="ciliated left-right organizer metallopeptidase"
                     /db_xref="GeneID:100128908"
                     /db_xref="HGNC:HGNC:53647"
                     /db_xref="MIM:619703"
     exon            1..391
                     /gene="CIROP"
                     /gene_synonym="HTX12; LMLN2"
                     /inference="alignment:Splign:2.1.0"
     CDS             70..2436
                     /gene="CIROP"
                     /gene_synonym="HTX12; LMLN2"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; leishmanolysin homolog; leishmanolysin like
                     peptidase 2"
                     /codon_start=1
                     /product="ciliated left-right organizer metallopeptidase
                     isoform 1 precursor"
                     /protein_id="NP_001341569.1"
                     /db_xref="CCDS:CCDS86373.1"
                     /db_xref="GeneID:100128908"
                     /db_xref="HGNC:HGNC:53647"
                     /db_xref="MIM:619703"
                     /translation="MLLLLLLLLLLPPLVLRVAASRCLHDETQKSVSLLRPPFSQLPS
                     KSRSSSLTLPSSRDPQPLRIQSCYLGDHISDGAWDPEGEGMRGGSRALAAVREATQRI
                     QAVLAVQGPLLLSRDPAQYCHAVWGDPDSPNYHRCSLLNPGYKGESCLGAKIPDTHLR
                     GYALWPEQGPPQLVQPDGPGVQNTDFLLYVRVAHTSKCHQETVSLCCPGWSTAAQSQL
                     TAALTSWAQRRGFVMLPRLCLKLLGSSNLPTLASQSIRITGPSVIAYAACCQLDSEDR
                     PLAGTIVYCAQHLTSPSLSHSDIVMATLHELLHALGFSGQLFKKWRDCPSGFSVRENC
                     STRQLVTRQDEWGQLLLTTPAVSLSLAKHLGVSGASLGVPLEEEEGLLSSHWEARLLQ
                     GSLMTATFDGAQRTRLDPITLAAFKDSGWYQVNHSAAEELLWGQGSGPEFGLVTTCGT
                     GSSDFFCTGSGLGCHYLHLDKGSCSSDPMLEGCRMYKPLANGSECWKKENGFPAGVDN
                     PHGEIYHPQSRCFFANLTSQLLPGDKPRHPSLTPHLKEAELMGRCYLHQCTGRGAYKV
                     QVEGSPWVPCLPGKVIQIPGYYGLLFCPRGRLCQTNEDINAVTSPPVSLSTPDPLFQL
                     SLELAGPPGHSLGKEQQEGLAEAVLEALASKGGTGRCYFHGPSITTSLVFTVHMWKSP
                     GCQGPSVATLHKALTLTLQKKPLEVYHGGANFTTQPSKLLVTSDHNPSMTHLRLSMGL
                     CLMLLILVGVMGTTAYQKRATLPVRPSASYHSPELHSTRVPVRGIREV"
     exon            392..476
                     /gene="CIROP"
                     /gene_synonym="HTX12; LMLN2"
                     /inference="alignment:Splign:2.1.0"
     exon            477..528
                     /gene="CIROP"
                     /gene_synonym="HTX12; LMLN2"
                     /inference="alignment:Splign:2.1.0"
     exon            529..672
                     /gene="CIROP"
                     /gene_synonym="HTX12; LMLN2"
                     /inference="alignment:Splign:2.1.0"
     exon            673..750
                     /gene="CIROP"
                     /gene_synonym="HTX12; LMLN2"
                     /inference="alignment:Splign:2.1.0"
     exon            751..846
                     /gene="CIROP"
                     /gene_synonym="HTX12; LMLN2"
                     /inference="alignment:Splign:2.1.0"
     exon            847..972
                     /gene="CIROP"
                     /gene_synonym="HTX12; LMLN2"
                     /inference="alignment:Splign:2.1.0"
     exon            973..1057
                     /gene="CIROP"
                     /gene_synonym="HTX12; LMLN2"
                     /inference="alignment:Splign:2.1.0"
     exon            1058..1203
                     /gene="CIROP"
                     /gene_synonym="HTX12; LMLN2"
                     /inference="alignment:Splign:2.1.0"
     exon            1204..1378
                     /gene="CIROP"
                     /gene_synonym="HTX12; LMLN2"
                     /inference="alignment:Splign:2.1.0"
     exon            1379..1448
                     /gene="CIROP"
                     /gene_synonym="HTX12; LMLN2"
                     /inference="alignment:Splign:2.1.0"
     exon            1449..1545
                     /gene="CIROP"
                     /gene_synonym="HTX12; LMLN2"
                     /inference="alignment:Splign:2.1.0"
     exon            1546..1818
                     /gene="CIROP"
                     /gene_synonym="HTX12; LMLN2"
                     /inference="alignment:Splign:2.1.0"
     exon            1819..2048
                     /gene="CIROP"
                     /gene_synonym="HTX12; LMLN2"
                     /inference="alignment:Splign:2.1.0"
     exon            2049..2228
                     /gene="CIROP"
                     /gene_synonym="HTX12; LMLN2"
                     /inference="alignment:Splign:2.1.0"
     exon            2229..2656
                     /gene="CIROP"
                     /gene_synonym="HTX12; LMLN2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2635..2640
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CIROP"
                     /gene_synonym="HTX12; LMLN2"
                     /note="hexamer: AATAAA"
     polyA_site      2656
                     /gene="CIROP"
                     /gene_synonym="HTX12; LMLN2"
                     /note="major polyA site"
ORIGIN      
        1 agtgagccag ggaacaaggg gacggaagac aggatccagg ctgtatctac ggagagacac
       61 agaagggaga tgctgctgct gctgctgctg ctgctgctgc tgccgccact agtcctcagg
      121 gttgctgcaa gccgatgtct acatgatgag acacagaagt ctgtgagcct tctcaggccc
      181 cctttctccc aactcccctc aaaatctcgc tcttcctccc tcaccctccc tagctcccgt
      241 gatcctcaac ccctacgaat ccaaagctgc tatctaggag atcatatatc agatggagct
      301 tgggatcctg agggagaagg gatgagaggg ggatcccgag ccctggccgc agtgagagag
      361 gccactcagc gaatccaggc tgttctagca gtgcaaggac ccctgcttct gagtcgagac
      421 cctgcacagt attgccacgc tgtctgggga gacccagata gcccaaacta ccacaggtgc
      481 agcctcttga acccaggata caaaggagag agttgcctgg gggcaaagat tcctgacacc
      541 catcttcgcg gttatgcctt gtggccggag cagggtcccc cacaactggt ccagccagat
      601 gggcctgggg tccaaaacac tgattttctc ctgtatgtgc gagttgctca cacttccaag
      661 tgccaccaag agacagtctc actctgttgc ccaggctgga gtacagcggc ccaatcacag
      721 ctcaccgcag ccttgacctc ctgggctcag agacggggtt ttgtcatgtt gcccaggctg
      781 tgtctcaaac tcttgggctc aagcaatctg cccaccttgg cttcccaaag tatcaggatt
      841 acaggcccct ctgtcatagc ctatgctgcc tgctgccagc tggactcaga agacaggccc
      901 ctcgctggta ccattgtcta ctgtgcccaa catctcacca gccccagcct cagccacagt
      961 gacatcgtca tggccacatt acatgaattg ctccatgcct tgggtttctc tggacagctc
     1021 ttcaagaaat ggcgagactg cccctcagga ttcagtgtta gagagaactg ttctacaagg
     1081 caactagtga caaggcaaga tgagtgggga caactgcttc tcaccacccc agctgttagc
     1141 ctcagcctgg ccaaacactt gggagtgtcg ggggcttccc tgggtgttcc cttggaagaa
     1201 gaggagggcc ttctgtcctc gcactgggag gccagactac tccagggttc tttaatgact
     1261 gctacctttg atggagccca gcgcactcga ctcgacccaa tcaccctcgc tgccttcaaa
     1321 gactcaggct ggtaccaggt caaccacagc gctgcagagg agctgttgtg gggccaggga
     1381 tctggcccag aatttggctt ggtgaccaca tgtgggactg gctcctcaga cttcttctgt
     1441 actggcagtg ggctgggctg ccactacctg cacctggaca agggaagctg ctcctcagac
     1501 cccatgctgg aaggctgccg catgtacaag cccttagcca atgggagtga atgctggaag
     1561 aaggaaaacg gattccctgc tggggtggat aatccccatg gggagatcta ccatccccag
     1621 agccgttgct tctttgccaa cctcacttca cagctgctcc ctggggataa gcccaggcat
     1681 ccttctctta ccccacacct caaggaagca gagctcatgg gccgctgcta cttacatcaa
     1741 tgcacaggga ggggagctta caaggtgcag gtggagggct cgccttgggt cccatgcctt
     1801 cctggaaagg ttatacagat acctgggtac tatggtcttc tcttctgtcc ccggggtcgg
     1861 ctgtgtcaga ctaatgaaga tatcaatgct gttacttccc cacctgtgag tctttcaacc
     1921 ccagatccac tattccagct ctctttagaa ttagctgggc ctccaggaca ctctctgggg
     1981 aaggaacagc aagaagggct agctgaagca gtactggagg ctttggcgag caaaggcggc
     2041 actggcaggt gctatttcca tggcccatca attaccacta gcttggtgtt tactgtgcat
     2101 atgtggaagt cccctggctg ccaagggcct tcagttgcta cactgcacaa ggccctgact
     2161 ctgactctcc agaaaaaacc cctagaagtg tatcatggag gagccaactt taccacacaa
     2221 cccagcaagt tgctggttac ttcagaccat aatccctcca tgacccacct aaggctgtcc
     2281 atgggactct gcctaatgct gctaatcctg gtgggtgtaa tgggaaccac agcctaccag
     2341 aaaagagcca ctcttcctgt gagaccatct gcctcttacc attcaccaga gctccacagc
     2401 acaagggtcc cagttagagg aataagggag gtgtgatgtt gcccagaaca tgacaggggg
     2461 taaggaagag aataatttct tgtgagacaa ctggatggaa aatctattgt gtatacttaa
     2521 tttctacttt ctttggaatg cctctttccc cggttgtcaa tattatatag cctggcaatg
     2581 aaaaaagggt gaagttttac agttctaaag atccattctt tattttggta caaaaataaa
     2641 ttgttcaacc tgtaaa
//
