LOCUS       NM_003613               5678 bp    mRNA    linear   PRI 06-JUN-2024
DEFINITION  Homo sapiens cartilage intermediate layer protein (CILP), mRNA.
ACCESSION   NM_003613
VERSION     NM_003613.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5678)
  AUTHORS   Guo,B., Zhao,F. and Zhang,S.
  TITLE     CILP is a potential pan-cancer marker: combined silico study and in
            vitro analyses
  JOURNAL   Cancer Gene Ther 31 (1), 119-130 (2024)
   PUBMED   37968343
  REMARK    GeneRIF: CILP is a potential pan-cancer marker: combined silico
            study and in vitro analyses.
REFERENCE   2  (bases 1 to 5678)
  AUTHORS   Weidenhammer,A., Prausmuller,S., Partsch,C., Spinka,G.,
            Luckerbauer,B., Larch,M., Arfsten,H., Abdel Mawgoud,R.,
            Bartko,P.E., Goliasch,G., Kastl,S., Hengstenberg,C., Hulsmann,M.
            and Pavo,N.
  TITLE     CILP-1 Is a Biomarker for Backward Failure and Right Ventricular
            Dysfunction in HFrEF
  JOURNAL   Cells 12 (24), 2832 (2023)
   PUBMED   38132152
  REMARK    GeneRIF: CILP-1 Is a Biomarker for Backward Failure and Right
            Ventricular Dysfunction in HFrEF.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5678)
  AUTHORS   S,M. H, M,K. S, G,K. C and I Bhat,D.
  TITLE     Association of CILP, COL9A2 and MMP3 Gene Polymorphisms with Lumbar
            Disc Degeneration in an Indian Population
  JOURNAL   J Mol Neurosci 66 (3), 378-382 (2018)
   PUBMED   30288688
  REMARK    GeneRIF: CILP gene polymorphism (rs2073711) is associated with a
            lower risk of lumbar disc degeneration.
REFERENCE   4  (bases 1 to 5678)
  AUTHORS   Taipale,M., Solovieva,S., Leino-Arjas,P. and Mannikko,M.
  TITLE     Functional polymorphisms in asporin and CILP together with joint
            loading predispose to hand osteoarthritis
  JOURNAL   BMC Genet 18 (1), 108 (2017)
   PUBMED   29233086
  REMARK    GeneRIF: CILP rs2073711 TT is associated with increased risk of
            symmetrical hand osteoarthritis particularly in individuals with
            low variation in work tasks.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 5678)
  AUTHORS   van Nieuwenhoven,F.A., Munts,C., Op't Veld,R.C., Gonzalez,A.,
            Diez,J., Heymans,S., Schroen,B. and van Bilsen,M.
  TITLE     Cartilage intermediate layer protein 1 (CILP1): A novel mediator of
            cardiac extracellular matrix remodelling
  JOURNAL   Sci Rep 7 (1), 16042 (2017)
   PUBMED   29167509
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 5678)
  AUTHORS   Johnson,K., Farley,D., Hu,S.I. and Terkeltaub,R.
  TITLE     One of two chondrocyte-expressed isoforms of cartilage
            intermediate-layer protein functions as an insulin-like growth
            factor 1 antagonist
  JOURNAL   Arthritis Rheum 48 (5), 1302-1314 (2003)
   PUBMED   12746903
REFERENCE   7  (bases 1 to 5678)
  AUTHORS   Lorenzo,P., Aman,P., Sommarin,Y. and Heinegard,D.
  TITLE     The human CILP gene: exon/intron organization and chromosomal
            mapping
  JOURNAL   Matrix Biol 18 (5), 445-454 (1999)
   PUBMED   10601732
REFERENCE   8  (bases 1 to 5678)
  AUTHORS   Nakamura,I., Okawa,A., Ikegawa,S., Takaoka,K. and Nakamura,Y.
  TITLE     Genomic organization, mapping, and polymorphisms of the gene
            encoding human cartilage intermediate layer protein (CILP)
  JOURNAL   J Hum Genet 44 (3), 203-205 (1999)
   PUBMED   10319588
REFERENCE   9  (bases 1 to 5678)
  AUTHORS   Lorenzo,P., Neame,P., Sommarin,Y. and Heinegard,D.
  TITLE     Cloning and deduced amino acid sequence of a novel cartilage
            protein (CILP) identifies a proform including a nucleotide
            pyrophosphohydrolase
  JOURNAL   J Biol Chem 273 (36), 23469-23475 (1998)
   PUBMED   9722584
REFERENCE   10 (bases 1 to 5678)
  AUTHORS   Lorenzo,P., Bayliss,M.T. and Heinegard,D.
  TITLE     A novel cartilage protein (CILP) present in the mid-zone of human
            articular cartilage increases with age
  JOURNAL   J Biol Chem 273 (36), 23463-23468 (1998)
   PUBMED   9722583
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC068213.7.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_003613.3.
            
            Summary: Major alterations in the composition of the cartilage
            extracellular matrix occur in joint disease, such as
            osteoarthrosis. This gene encodes the cartilage intermediate layer
            protein (CILP), which increases in early osteoarthrosis cartilage.
            The encoded protein was thought to encode a protein precursor for
            two different proteins; an N-terminal CILP and a C-terminal homolog
            of NTPPHase, however, later studies identified no nucleotide
            pyrophosphatase phosphodiesterase (NPP) activity. The full-length
            and the N-terminal domain of this protein was shown to function as
            an IGF-1 antagonist. An allelic variant of this gene has been
            associated with lumbar disc disease. [provided by RefSeq, Sep
            2010].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            transcript and genomic sequence to make the sequence consistent
            with the reference genome assembly. The genomic coordinates used
            for the transcript record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK313352.1, AY358904.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2467146, SAMEA2467148
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000261883.6/ ENSP00000261883.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-46                AC068213.7         85105-85150
            47-213              AC068213.7         86717-86883
            214-306             AC068213.7         88814-88906
            307-576             AC068213.7         89527-89796
            577-756             AC068213.7         91112-91291
            757-1071            AC068213.7         91996-92310
            1072-1180           AC068213.7         93108-93216
            1181-1338           AC068213.7         94549-94706
            1339-5678           AC068213.7         97479-101818
FEATURES             Location/Qualifiers
     source          1..5678
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="15"
                     /map="15q22.31"
     gene            1..5678
                     /gene="CILP"
                     /gene_synonym="CILP-1; CILP1; HsT18872"
                     /note="cartilage intermediate layer protein"
                     /db_xref="GeneID:8483"
                     /db_xref="HGNC:HGNC:1980"
                     /db_xref="MIM:603489"
     exon            1..46
                     /gene="CILP"
                     /gene_synonym="CILP-1; CILP1; HsT18872"
                     /inference="alignment:Splign:2.1.0"
     exon            47..213
                     /gene="CILP"
                     /gene_synonym="CILP-1; CILP1; HsT18872"
                     /inference="alignment:Splign:2.1.0"
     CDS             153..3707
                     /gene="CILP"
                     /gene_synonym="CILP-1; CILP1; HsT18872"
                     /note="cartilage intermediate layer protein 1 C1;
                     cartilage intermediate layer protein 1 C2; cartilage
                     intermediate layer protein, nucleotide
                     pyrophosphohydrolase"
                     /codon_start=1
                     /product="cartilage intermediate layer protein 1
                     preproprotein"
                     /protein_id="NP_003604.4"
                     /db_xref="CCDS:CCDS10203.1"
                     /db_xref="GeneID:8483"
                     /db_xref="HGNC:HGNC:1980"
                     /db_xref="MIM:603489"
                     /translation="MVGTKAWVFSFLVLEVTSVLGRQTMLTQSVRRVQPGKKNPSIFA
                     KPADTLESPGEWTTWFNIDYPGGKGDYERLDAIRFYYGDRVCARPLRLEARTTDWTPA
                     GSTGQVVHGSPREGFWCLNREQRPGQNCSNYTVRFLCPPGSLRRDTERIWSPWSPWSK
                     CSAACGQTGVQTRTRICLAEMVSLCSEASEEGQHCMGQDCTACDLTCPMGQVNADCDA
                     CMCQDFMLHGAVSLPGGAPASGAAIYLLTKTPKLLTQTDSDGRFRIPGLCPDGKSILK
                     ITKVKFAPIVLTMPKTSLKAATIKAEFVRAETPYMVMNPETKARRAGQSVSLCCKATG
                     KPRPDKYFWYHNDTLLDPSLYKHESKLVLRKLQQHQAGEYFCKAQSDAGAVKSKVAQL
                     IVIASDETPCNPVPESYLIRLPHDCFQNATNSFYYDVGRCPVKTCAGQQDNGIRCRDA
                     VQNCCGISKTEEREIQCSGYTLPTKVAKECSCQRCTETRSIVRGRVSAADNGEPMRFG
                     HVYMGNSRVSMTGYKGTFTLHVPQDTERLVLTFVDRLQKFVNTTKVLPFNKKGSAVFH
                     EIKMLRRKKPITLEAMETNIIPLGEVVGEDPMAELEIPSRSFYRQNGEPYIGKVKASV
                     TFLDPRNISTATAAQTDLNFINDEGDTFPLRTYGMFSVDFRDEVTSEPLNAGKVKVHL
                     DSTQVKMPEHISTVKLWSLNPDTGLWEEEGDFKFENQRRNKREDRTFLVGNLEIRERR
                     LFNLDVPESRRCFVKVRAYRSERFLPSEQIQGVVISVINLEPRTGFLSNPRAWGRFDS
                     VITGPNGACVPAFCDDQSPDAYSAYVLASLAGEELQAVESSPKFNPNAIGVPQPYLNK
                     LNYRRTDHEDPRVKKTAFQISMAKPRPNSAEESNGPIYAFENLRACEEAPPSAAHFRF
                     YQIEGDRYDYNTVPFNEDDPMSWTEDYLAWWPKPMEFRACYIKVKIVGPLEVNVRSRN
                     MGGTHRQTVGKLYGIRDVRSTRDRDQPNVSAACLEFKCSGMLYDQDRVDRTLVKVIPQ
                     GSCRRASVNPMLHEYLVNHLPLAVNNDTSEYTMLAPLDPLGHNYGIYTVTDQDPRTAK
                     EIALGRCFDGTSDGSSRIMKSNVGVALTFNCVERQVGRQSAFQYLQSTPAQSPAAGTV
                     QGRVPSRRQQRASRGGQRQGGVVASLRFPRVAQQPLIN"
     exon            214..306
                     /gene="CILP"
                     /gene_synonym="CILP-1; CILP1; HsT18872"
                     /inference="alignment:Splign:2.1.0"
     exon            307..576
                     /gene="CILP"
                     /gene_synonym="CILP-1; CILP1; HsT18872"
                     /inference="alignment:Splign:2.1.0"
     exon            577..756
                     /gene="CILP"
                     /gene_synonym="CILP-1; CILP1; HsT18872"
                     /inference="alignment:Splign:2.1.0"
     exon            757..1071
                     /gene="CILP"
                     /gene_synonym="CILP-1; CILP1; HsT18872"
                     /inference="alignment:Splign:2.1.0"
     exon            1072..1180
                     /gene="CILP"
                     /gene_synonym="CILP-1; CILP1; HsT18872"
                     /inference="alignment:Splign:2.1.0"
     exon            1181..1338
                     /gene="CILP"
                     /gene_synonym="CILP-1; CILP1; HsT18872"
                     /inference="alignment:Splign:2.1.0"
     exon            1339..5678
                     /gene="CILP"
                     /gene_synonym="CILP-1; CILP1; HsT18872"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4155..4160
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CILP"
                     /gene_synonym="CILP-1; CILP1; HsT18872"
                     /note="hexamer: AATAAA"
     regulatory      4163..4168
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CILP"
                     /gene_synonym="CILP-1; CILP1; HsT18872"
                     /note="hexamer: AATAAA"
     polyA_site      4174
                     /gene="CILP"
                     /gene_synonym="CILP-1; CILP1; HsT18872"
     polyA_site      4189
                     /gene="CILP"
                     /gene_synonym="CILP-1; CILP1; HsT18872"
                     /note="major polyA site"
     regulatory      4408..4413
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CILP"
                     /gene_synonym="CILP-1; CILP1; HsT18872"
                     /note="hexamer: AATAAA"
     polyA_site      4438
                     /gene="CILP"
                     /gene_synonym="CILP-1; CILP1; HsT18872"
     regulatory      5657..5662
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CILP"
                     /gene_synonym="CILP-1; CILP1; HsT18872"
                     /note="hexamer: AATAAA"
     polyA_site      5678
                     /gene="CILP"
                     /gene_synonym="CILP-1; CILP1; HsT18872"
ORIGIN      
        1 aggcgaaggc ttggaccccc agctgaggag tcctgctcaa gacacggtca ctggatctga
       61 gaaacttccc aggggaccgc attccagagt cagtgactct gtgaagcacc cacatctacc
      121 tcttgccacg ttcccacggg cttgggggaa agatggtggg gaccaaggcc tgggtgttct
      181 ccttcctggt cctggaagtc acatctgtgt tggggagaca gacgatgctc acccagtcag
      241 taagaagagt ccagcctggg aagaagaacc ccagcatctt tgccaagcct gccgacaccc
      301 tggagagccc tggtgagtgg acaacatggt tcaacatcga ctacccaggc gggaagggcg
      361 actatgagcg gctggacgcc attcgcttct actatgggga ccgtgtatgt gcccgtcccc
      421 tgcggctaga ggctcggacc actgactgga cacctgcggg cagcactggc caggtggtcc
      481 atggtagtcc ccgtgagggt ttctggtgcc tcaacaggga gcagcggcct ggccagaact
      541 gctctaatta caccgtacgc ttcctctgcc caccaggatc cctgcgccga gacacagagc
      601 gcatctggag cccatggtct ccctggagca agtgctcagc tgcctgtggt cagactgggg
      661 tccagactcg cacacgcatt tgcttggcag agatggtgtc gctgtgcagt gaggccagcg
      721 aagagggtca gcactgcatg ggccaggact gtacagcctg tgacctgacc tgcccaatgg
      781 gccaggtgaa tgctgactgt gatgcctgca tgtgccagga cttcatgctt catggggctg
      841 tctcccttcc cggaggtgcc ccagcctcag gggctgctat ctacctcctg accaagacgc
      901 cgaagctgct gacccagaca gacagtgatg ggagattccg aatccctggc ttgtgccctg
      961 atggcaaaag catcctgaag atcacaaagg tcaagtttgc ccccattgta ctcacaatgc
     1021 ccaagactag cctgaaggca gccaccatca aggcagagtt tgtgagggca gagactccat
     1081 acatggtgat gaaccctgag acaaaagcac ggagagctgg gcagagcgtg tctctgtgct
     1141 gtaaggccac agggaagccc aggccagaca agtatttttg gtatcataat gacacattgc
     1201 tggatccttc cctctacaag catgagagca agctggtgct gaggaaactg cagcagcacc
     1261 aggctgggga gtacttttgc aaggcccaga gtgatgctgg ggctgtgaag tccaaggttg
     1321 cccagctgat tgtcatagca tctgatgaga ctccttgcaa cccagttcct gagagctatc
     1381 ttatccggct gccccatgat tgctttcaga atgccaccaa ctccttctac tatgacgtgg
     1441 gacgctgccc tgttaagact tgtgcagggc agcaggataa tgggatcagg tgccgtgatg
     1501 ctgtgcagaa ctgctgtggc atctccaaga cagaggaaag ggagatccag tgcagtggct
     1561 acacgctacc caccaaggtg gccaaggagt gcagctgcca gcggtgtacg gaaactcgga
     1621 gcatcgtgcg gggccgtgtc agtgctgctg acaatgggga gcccatgcgc tttggccatg
     1681 tgtacatggg gaacagccgt gtaagcatga ctggctacaa gggcactttc accctccatg
     1741 tcccccagga cactgagagg ctggtgctca catttgtgga caggctgcag aagtttgtca
     1801 acaccaccaa agtgctacct ttcaacaaga aggggagtgc cgtgttccat gaaatcaaga
     1861 tgcttcgtcg gaaaaagccc atcactttgg aagccatgga gaccaacatc atccccctgg
     1921 gggaagtggt tggtgaagac cccatggctg aactggagat tccatccagg agtttctaca
     1981 ggcagaatgg ggagccctac ataggaaaag tgaaggccag tgtgaccttc ctggatcccc
     2041 ggaatatttc cacagccaca gctgcccaga ctgacctgaa cttcatcaat gacgaaggag
     2101 acactttccc ccttcggacg tatggcatgt tctctgtgga cttcagagat gaggtcacct
     2161 cagagccact taatgctggc aaagtgaagg tccaccttga ctcgacccag gtcaagatgc
     2221 cagagcacat atccacagtg aaactctggt cactcaatcc agacacaggg ctgtgggagg
     2281 aggaaggtga tttcaaattt gaaaatcaaa ggaggaacaa aagagaagac agaaccttcc
     2341 tggtgggcaa cctggagatt cgtgagagga ggctctttaa cctggatgtt cctgaaagca
     2401 ggcggtgctt tgttaaggtg agggcctacc ggagtgagag gttcttgcct agtgagcaga
     2461 tccagggggt tgtgatctcc gtgattaacc tggagcctag aactggcttc ttgtccaacc
     2521 ctagggcctg gggccgcttt gacagtgtca tcacaggccc caacggggcc tgtgtgcctg
     2581 ccttctgtga tgaccagtcc cctgatgcct actctgccta tgtcttggca agcctggctg
     2641 gggaggaact gcaagcagtg gagtcttctc ctaaattcaa cccaaatgca attggcgtcc
     2701 ctcagcccta tctcaacaag ctcaactacc gtcggacgga ccatgaggat ccacgggtta
     2761 aaaagacagc tttccagatt agcatggcca agccaaggcc caactcagct gaggagagca
     2821 atgggcccat ctatgccttt gagaacctcc gggcatgtga agaggcacca cccagtgcag
     2881 cccacttccg gttctaccag attgaggggg atcgatatga ctacaacaca gtccccttca
     2941 acgaagatga ccctatgagc tggactgaag actatctggc atggtggcca aagccgatgg
     3001 aattcagggc ctgctatatc aaggtgaaga ttgtggggcc actggaagtg aatgtgcgat
     3061 cccgcaacat ggggggcact catcggcaga cagtggggaa gctgtatgga atccgagatg
     3121 tgaggagcac tcgggacagg gaccagccca atgtctcagc tgcctgtctg gagttcaagt
     3181 gcagtgggat gctctatgat caggaccgtg tggaccgcac cctggtgaag gtcatccccc
     3241 agggcagctg ccgtcgagcc agtgtgaacc ccatgctgca tgagtacctg gtcaaccact
     3301 tgccacttgc agtcaacaac gacaccagtg agtacaccat gctggcaccc ttggacccac
     3361 tgggccacaa ctatggcatc tacactgtca ctgaccagga ccctcgcacg gccaaggaga
     3421 tcgcgctcgg ccggtgcttt gatggcacat ccgatggctc ctccagaatc atgaagagca
     3481 atgtgggagt agccctcacc ttcaactgtg tagagaggca agtaggccgc cagagtgcct
     3541 tccagtacct ccaaagcacc ccagcccagt cccctgctgc aggcactgtc caaggaagag
     3601 tgccctcgag gaggcagcag cgagcgagca ggggtggcca gcgccagggt ggagtggtgg
     3661 cctctctgag atttcctaga gttgctcaac agcccctgat caactaagtt ttgtggtact
     3721 tcaccctctt ctgccctcat ttcatgtgac agccattgtg agactgatgc acaaactgtc
     3781 acttggttaa tttaagcact tctgttttcg tgaatttgct tgtttgtttc ttcatgcctt
     3841 tacttacttt gtcccatgct actgattggc acgtggcccc cacaatggca caataaagcc
     3901 cctttgtgaa actgttcttt aaatgaaaca caagaaattg gccactggta aaactctgca
     3961 gcttcaactg tacttcattt aatgccatta atgcaaatat acttcctctt ctttttgcat
     4021 ggttttgccc acctctgcaa tagtgataat ctgatgctga agatcaaata accaatataa
     4081 agcatatttc ttggccttgc tccacaggac ataggcaagc cttgatcata gttcatacat
     4141 ataaatggtg gtgaaataaa gaaataaaac acaatacttt tacttgaaat gtaaataact
     4201 tatttatttc tttgctaaat ttggaattct agtgcacatt caaagttaag ctattaaata
     4261 tagggtgatc atagttcctc taccaagtct ggaaagaaca tctcctggta tccacaatta
     4321 caccaggttg ctaactgtat ttgtacattt ccctttgcat tcgcttctgt tcttgctaga
     4381 aacccagtgt agcccagggc agatgtcaat aaatgcatac tctgtatttc gattctgagg
     4441 ccactgtcct gtcttaacta ttaaagtgag ccagcttctc agaggagtac aggggactct
     4501 ggaaagcagt cacaggtggt gtaagtcctc gccccctaag ttacagggca gggcaacata
     4561 ggcacacact ccagcagtca aatttgttct catggatcac attttccgtt tgcagaaaag
     4621 agcagattcc tggagttgtg agcatgttct gggctcctga gcaagactaa aaacacgtcc
     4681 cgattttttt ctttccttct ttccaatact ttggagctta tacaatggct tggcccaggg
     4741 tctgacaagc tggccaagtg gtcctttggc tctcccaaca gcctgttgcc ttctccagct
     4801 gggctggccg gcctcactca gaaatgggct tttacttttt tctttatgct cttctaaaga
     4861 tgttcaactt ttccagacac cagatctctg ttgtgtgtta cagaccctgt ccctggagca
     4921 aaacaattgg tccaaacggg cagagttttg gttttggaaa aggtatttgg tttaaaatag
     4981 taaatcagag gcccataggt ggtgccagag cgatctcagc ggcattacag tcagggtagt
     5041 ggggtgtggg gttggatagg atgcactcac tgcccctatc ccaacccctc cagaatctgt
     5101 caggcaagag tccaaagggt ttgggagaag agaggacact gtttacaatc aggacaaaaa
     5161 gctttccctg atgccctggc cagcctagtg cagctccttt acccatctct ggcctgggct
     5221 ggatttgtac tcttggatca ccatctgccc ggctcctggg aatttggttt gtaagcaatt
     5281 agaaatttct ctagctgagc acatcacaat aaccatagaa aaagcaagca tctgcaggag
     5341 tgaggactag tggtcttgtg attgcaactt gacccacttc cagactccac atctgtccca
     5401 ctgctcccag gaagcagctc tcactgactg cttggggagg cttgactgcc cctgttgtga
     5461 gtgcacagct atctgcagca ccatcgcccc agccacttca ggagccagag tgcctgctgc
     5521 ttcaacctga ccaccagttc acttcaaact cacgggggga gggggagggt cggggggggg
     5581 tgggttgctg gggaaggtgg gctgactggt tccccactct agggcacttt ccttgctgtt
     5641 ctcaattgag ggcctgaata aatggactcc agcacaca
//
