LOCUS       NM_001375400            6270 bp    mRNA    linear   PRI 21-SEP-2024
DEFINITION  Homo sapiens ciliogenesis associated kinase 1 (CILK1), transcript
            variant 5, mRNA.
ACCESSION   NM_001375400 XM_017010490
VERSION     NM_001375400.1
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6270)
  AUTHORS   He,Y., Zhang,X., Pan,W., Zhang,J., Zhu,W., Zhang,J. and Shi,J.
  TITLE     Ciliogenesis-associated Kinase 1 Promotes Breast Cancer Cell
            Proliferation and Chemoresistance via Phosphorylating ERK1
  JOURNAL   Int J Biol Sci 20 (7), 2403-2421 (2024)
   PUBMED   38725848
  REMARK    GeneRIF: Ciliogenesis-associated Kinase 1 Promotes Breast Cancer
            Cell Proliferation and Chemoresistance via Phosphorylating ERK1.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6270)
  AUTHORS   Turner,J.S., McCabe,E.A., Kuang,K.W., Gailey,C.D., Brautigan,D.L.,
            Limerick,A., Wang,E.X. and Fu,Z.
  TITLE     The Scaffold Protein KATNIP Enhances CILK1 Control of Primary Cilia
  JOURNAL   Mol Cell Biol 43 (9), 472-480 (2023)
   PUBMED   37665596
  REMARK    GeneRIF: The Scaffold Protein KATNIP Enhances CILK1 Control of
            Primary Cilia.
REFERENCE   3  (bases 1 to 6270)
  AUTHORS   Satoda,Y., Noguchi,T., Fujii,T., Taniguchi,A., Katoh,Y. and
            Nakayama,K.
  TITLE     BROMI/TBC1D32 together with CCRK/CDK20 and FAM149B1/JBTS36
            contributes to intraflagellar transport turnaround involving
            ICK/CILK1
  JOURNAL   Mol Biol Cell 33 (9), ar79 (2022)
   PUBMED   35609210
  REMARK    GeneRIF: BROMI/TBC1D32 together with CCRK/CDK20 and FAM149B1/JBTS36
            contributes to intraflagellar transport turnaround involving
            ICK/CILK1.
REFERENCE   4  (bases 1 to 6270)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   5  (bases 1 to 6270)
  AUTHORS   Nakamura,K., Noguchi,T., Takahara,M., Omori,Y., Furukawa,T.,
            Katoh,Y. and Nakayama,K.
  TITLE     Anterograde trafficking of ciliary MAP kinase-like ICK/CILK1 by the
            intraflagellar transport machinery is required for intraciliary
            retrograde protein trafficking
  JOURNAL   J Biol Chem 295 (38), 13363-13376 (2020)
   PUBMED   32732286
  REMARK    GeneRIF: Anterograde trafficking of ciliary MAP kinase-like
            ICK/CILK1 by the intraflagellar transport machinery is required for
            intraciliary retrograde protein trafficking.
REFERENCE   6  (bases 1 to 6270)
  AUTHORS   Fu,Z., Schroeder,M.J., Shabanowitz,J., Kaldis,P., Togawa,K.,
            Rustgi,A.K., Hunt,D.F. and Sturgill,T.W.
  TITLE     Activation of a nuclear Cdc2-related kinase within a
            mitogen-activated protein kinase-like TDY motif by
            autophosphorylation and cyclin-dependent protein kinase-activating
            kinase
  JOURNAL   Mol Cell Biol 25 (14), 6047-6064 (2005)
   PUBMED   15988018
  REMARK    GeneRIF: ICK is the prototype for a new group of MAPK-like kinases
            requiring dual phosphorylation at TDY motifs.
REFERENCE   7  (bases 1 to 6270)
  AUTHORS   Colland,F., Jacq,X., Trouplin,V., Mougin,C., Groizeleau,C.,
            Hamburger,A., Meil,A., Wojcik,J., Legrain,P. and Gauthier,J.M.
  TITLE     Functional proteomics mapping of a human signaling pathway
  JOURNAL   Genome Res 14 (7), 1324-1332 (2004)
   PUBMED   15231748
REFERENCE   8  (bases 1 to 6270)
  AUTHORS   Mungall,A.J., Palmer,S.A., Sims,S.K., Edwards,C.A., Ashurst,J.L.,
            Wilming,L., Jones,M.C., Horton,R., Hunt,S.E., Scott,C.E.,
            Gilbert,J.G., Clamp,M.E., Bethel,G., Milne,S., Ainscough,R.,
            Almeida,J.P., Ambrose,K.D., Andrews,T.D., Ashwell,R.I.,
            Babbage,A.K., Bagguley,C.L., Bailey,J., Banerjee,R., Barker,D.J.,
            Barlow,K.F., Bates,K., Beare,D.M., Beasley,H., Beasley,O.,
            Bird,C.P., Blakey,S., Bray-Allen,S., Brook,J., Brown,A.J.,
            Brown,J.Y., Burford,D.C., Burrill,W., Burton,J., Carder,C.,
            Carter,N.P., Chapman,J.C., Clark,S.Y., Clark,G., Clee,C.M.,
            Clegg,S., Cobley,V., Collier,R.E., Collins,J.E., Colman,L.K.,
            Corby,N.R., Coville,G.J., Culley,K.M., Dhami,P., Davies,J.,
            Dunn,M., Earthrowl,M.E., Ellington,A.E., Evans,K.A., Faulkner,L.,
            Francis,M.D., Frankish,A., Frankland,J., French,L., Garner,P.,
            Garnett,J., Ghori,M.J., Gilby,L.M., Gillson,C.J., Glithero,R.J.,
            Grafham,D.V., Grant,M., Gribble,S., Griffiths,C., Griffiths,M.,
            Hall,R., Halls,K.S., Hammond,S., Harley,J.L., Hart,E.A.,
            Heath,P.D., Heathcott,R., Holmes,S.J., Howden,P.J., Howe,K.L.,
            Howell,G.R., Huckle,E., Humphray,S.J., Humphries,M.D., Hunt,A.R.,
            Johnson,C.M., Joy,A.A., Kay,M., Keenan,S.J., Kimberley,A.M.,
            King,A., Laird,G.K., Langford,C., Lawlor,S., Leongamornlert,D.A.,
            Leversha,M., Lloyd,C.R., Lloyd,D.M., Loveland,J.E., Lovell,J.,
            Martin,S., Mashreghi-Mohammadi,M., Maslen,G.L., Matthews,L.,
            McCann,O.T., McLaren,S.J., McLay,K., McMurray,A., Moore,M.J.,
            Mullikin,J.C., Niblett,D., Nickerson,T., Novik,K.L., Oliver,K.,
            Overton-Larty,E.K., Parker,A., Patel,R., Pearce,A.V., Peck,A.I.,
            Phillimore,B., Phillips,S., Plumb,R.W., Porter,K.M., Ramsey,Y.,
            Ranby,S.A., Rice,C.M., Ross,M.T., Searle,S.M., Sehra,H.K.,
            Sheridan,E., Skuce,C.D., Smith,S., Smith,M., Spraggon,L.,
            Squares,S.L., Steward,C.A., Sycamore,N., Tamlyn-Hall,G., Tester,J.,
            Theaker,A.J., Thomas,D.W., Thorpe,A., Tracey,A., Tromans,A.,
            Tubby,B., Wall,M., Wallis,J.M., West,A.P., White,S.S.,
            Whitehead,S.L., Whittaker,H., Wild,A., Willey,D.J., Wilmer,T.E.,
            Wood,J.M., Wray,P.W., Wyatt,J.C., Young,L., Younger,R.M.,
            Bentley,D.R., Coulson,A., Durbin,R., Hubbard,T., Sulston,J.E.,
            Dunham,I., Rogers,J. and Beck,S.
  TITLE     The DNA sequence and analysis of human chromosome 6
  JOURNAL   Nature 425 (6960), 805-811 (2003)
   PUBMED   14574404
REFERENCE   9  (bases 1 to 6270)
  AUTHORS   Yang,T., Jiang,Y. and Chen,J.
  TITLE     The identification and subcellular localization of human MRK
  JOURNAL   Biomol Eng 19 (1), 1-4 (2002)
   PUBMED   12103360
REFERENCE   10 (bases 1 to 6270)
  AUTHORS   Togawa,K., Yan,Y.X., Inomoto,T., Slaugenhaupt,S. and Rustgi,A.K.
  TITLE     Intestinal cell kinase (ICK) localizes to the crypt region and
            requires a dual phosphorylation site found in map kinases
  JOURNAL   J Cell Physiol 183 (1), 129-139 (2000)
   PUBMED   10699974
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL031178.1 and AL162581.11.
            
            On Oct 25, 2019 this sequence version replaced XM_017010490.1.
            
            Summary: Eukaryotic protein kinases are enzymes that belong to a
            very extensive family of proteins which share a conserved catalytic
            core common with both serine/threonine and tyrosine protein
            kinases. This gene encodes an intestinal serine/threonine kinase
            harboring a dual phosphorylation site found in mitogen-activating
            protein (MAP) kinases. The protein localizes to the intestinal
            crypt region and is thought to be important in intestinal
            epithelial cell proliferation and differentiation. Alternative
            splicing has been observed at this locus and two variants, encoding
            the same isoform, have been identified. [provided by RefSeq, Jul
            2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038194.1354035.1,
                                           SRR18074967.2253141.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-229               AL031178.1         38426-38654         c
            230-353             AL031178.1         22496-22619         c
            354-626             AL031178.1         17966-18238         c
            627-681             AL031178.1         14769-14823         c
            682-803             AL031178.1         9363-9484           c
            804-883             AL031178.1         7895-7974           c
            884-1016            AL162581.11        29950-30082         c
            1017-1188           AL162581.11        29053-29224         c
            1189-1356           AL162581.11        26806-26973         c
            1357-1677           AL162581.11        24385-24705         c
            1678-1868           AL162581.11        22760-22950         c
            1869-2017           AL162581.11        22492-22640         c
            2018-2146           AL162581.11        20162-20290         c
            2147-2269           AL162581.11        17038-17160         c
            2270-6270           AL162581.11        12026-16026         c
FEATURES             Location/Qualifiers
     source          1..6270
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6p12.1"
     gene            1..6270
                     /gene="CILK1"
                     /gene_synonym="ECO; EJM10; hICK; ICK; LCK2; MRK"
                     /note="ciliogenesis associated kinase 1"
                     /db_xref="GeneID:22858"
                     /db_xref="HGNC:HGNC:21219"
                     /db_xref="MIM:612325"
     exon            1..229
                     /gene="CILK1"
                     /gene_synonym="ECO; EJM10; hICK; ICK; LCK2; MRK"
                     /inference="alignment:Splign:2.1.0"
     exon            230..353
                     /gene="CILK1"
                     /gene_synonym="ECO; EJM10; hICK; ICK; LCK2; MRK"
                     /inference="alignment:Splign:2.1.0"
     exon            354..626
                     /gene="CILK1"
                     /gene_synonym="ECO; EJM10; hICK; ICK; LCK2; MRK"
                     /inference="alignment:Splign:2.1.0"
     CDS             526..2424
                     /gene="CILK1"
                     /gene_synonym="ECO; EJM10; hICK; ICK; LCK2; MRK"
                     /EC_number="2.7.11.1"
                     /note="isoform 1 is encoded by transcript variant 5;
                     MAK-related kinase; serine/threonine-protein kinase ICK;
                     serine/threonine protein kinase; laryngeal cancer kinase
                     2; intestinal cell kinase; iciliogenesis associated kinase
                     1; intestinal cell (MAK-like) kinase"
                     /codon_start=1
                     /product="serine/threonine-protein kinase ICK isoform 1"
                     /protein_id="NP_001362329.1"
                     /db_xref="CCDS:CCDS4949.1"
                     /db_xref="GeneID:22858"
                     /db_xref="HGNC:HGNC:21219"
                     /db_xref="MIM:612325"
                     /translation="MNRYTTIRQLGDGTYGSVLLGRSIESGELIAIKKMKRKFYSWEE
                     CMNLREVKSLKKLNHANVVKLKEVIRENDHLYFIFEYMKENLYQLIKERNKLFPESAI
                     RNIMYQILQGLAFIHKHGFFHRDLKPENLLCMGPELVKIADFGLAREIRSKPPYTDYV
                     STRWYRAPEVLLRSTNYSSPIDVWAVGCIMAEVYTLRPLFPGASEIDTIFKICQVLGT
                     PKKTDWPEGYQLSSAMNFRWPQCVPNNLKTLIPNASSEAVQLLRDMLQWDPKKRPTAS
                     QALRYPYFQVGHPLGSTTQNLQDSEKPQKGILEKAGPPPYIKPVPPAQPPAKPHTRIS
                     SRQHQASQPPLHLTYPYKAEVSRTDHPSHLQEDKPSPLLFPSLHNKHPQSKITAGLEH
                     KNGEIKPKSRRRWGLISRSTKDSDDWADLDDLDFSPSLSRIDLKNKKRQSDDTLCRFE
                     SVLDLKPSEPVGTGNSAPTQTSYQRRDTPTLRSAAKQHYLKHSRYLPGISIRNGILSN
                     PGKEFIPPNPWSSSGLSGKSSGTMSVISKVNSVGSSSTSSSGLTGNYVPSFLKKEIGS
                     AMQRVHLAPIPDPSPGYSSLKAMRPHPGRPFFHTQPRSTPGLIPRPPAAQPVHGRTDW
                     ASKYASRR"
     exon            627..681
                     /gene="CILK1"
                     /gene_synonym="ECO; EJM10; hICK; ICK; LCK2; MRK"
                     /inference="alignment:Splign:2.1.0"
     exon            682..803
                     /gene="CILK1"
                     /gene_synonym="ECO; EJM10; hICK; ICK; LCK2; MRK"
                     /inference="alignment:Splign:2.1.0"
     exon            804..883
                     /gene="CILK1"
                     /gene_synonym="ECO; EJM10; hICK; ICK; LCK2; MRK"
                     /inference="alignment:Splign:2.1.0"
     exon            884..1016
                     /gene="CILK1"
                     /gene_synonym="ECO; EJM10; hICK; ICK; LCK2; MRK"
                     /inference="alignment:Splign:2.1.0"
     exon            1017..1188
                     /gene="CILK1"
                     /gene_synonym="ECO; EJM10; hICK; ICK; LCK2; MRK"
                     /inference="alignment:Splign:2.1.0"
     exon            1189..1356
                     /gene="CILK1"
                     /gene_synonym="ECO; EJM10; hICK; ICK; LCK2; MRK"
                     /inference="alignment:Splign:2.1.0"
     exon            1357..1677
                     /gene="CILK1"
                     /gene_synonym="ECO; EJM10; hICK; ICK; LCK2; MRK"
                     /inference="alignment:Splign:2.1.0"
     exon            1678..1868
                     /gene="CILK1"
                     /gene_synonym="ECO; EJM10; hICK; ICK; LCK2; MRK"
                     /inference="alignment:Splign:2.1.0"
     exon            1869..2017
                     /gene="CILK1"
                     /gene_synonym="ECO; EJM10; hICK; ICK; LCK2; MRK"
                     /inference="alignment:Splign:2.1.0"
     exon            2018..2146
                     /gene="CILK1"
                     /gene_synonym="ECO; EJM10; hICK; ICK; LCK2; MRK"
                     /inference="alignment:Splign:2.1.0"
     exon            2147..2269
                     /gene="CILK1"
                     /gene_synonym="ECO; EJM10; hICK; ICK; LCK2; MRK"
                     /inference="alignment:Splign:2.1.0"
     exon            2270..6270
                     /gene="CILK1"
                     /gene_synonym="ECO; EJM10; hICK; ICK; LCK2; MRK"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6247..6252
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CILK1"
                     /gene_synonym="ECO; EJM10; hICK; ICK; LCK2; MRK"
                     /note="hexamer: AATAAA"
     polyA_site      6270
                     /gene="CILK1"
                     /gene_synonym="ECO; EJM10; hICK; ICK; LCK2; MRK"
                     /note="major polyA site"
ORIGIN      
        1 gtttgttttc ctgccggagg ctggaaaacg gccgggttcc tgctgcacta ccatgcgccg
       61 tgcggcccgt gcgactcgcc ggacctcgcg ggcgtccctg tacggagccc tcggccggtc
      121 ctagcaggga ttgtccccat ttccagctcc ggagcgggcg gctgcgcccc gctcgtcgag
      181 gagctgcgct cacctcaggg gcgggccccc gcctgcgttc gcggcgccag aagatgacat
      241 acaacgatgg tttgggagtc ttaatttgtt ggaaagtgaa ataatgaatg atggaggatt
      301 catacacaga tgtgtctcaa cttagagtgg ggttacatcc ctataaaccc attgcagaag
      361 actgattttt ggaaatatgt atttgggaga cagtcacgtc ctattgaata ccttgtgctg
      421 gtgctgccat cgaaaaatct ggttacactc tggggaggac tgctaccact gcagaactga
      481 accacttcgg ccgtgagatg agtgtccggc ctgagcaggc acaccatgaa tagatacaca
      541 acaatcaggc agctcgggga tggaacctac ggttccgtcc tgctgggaag aagcattgag
      601 tctggggagc tgatcgctat taaaaaaatg aaaagaaaat tttattcctg ggaggaatgc
      661 atgaaccttc gggaggttaa gtctttaaag aagctcaacc atgccaatgt agtcaaatta
      721 aaagaagtta tcagggaaaa tgatcatctt tattttatct tcgagtacat gaaggaaaat
      781 ctttaccagc tcattaaaga gagaaataag ttgtttcctg agtctgctat aaggaatatc
      841 atgtatcaga tattacaagg actcgcattt attcacaaac acggcttctt tcatcgagac
      901 ttaaagcctg agaacctcct ctgcatggga ccagaacttg tgaaaattgc agactttggt
      961 ttggcccgag aaatacgatc aaaacctcca tatacagatt atgtatctac cagatggtac
     1021 agggctccag aagtactcct gaggtctacc aactacagct cccccattga cgtctgggcg
     1081 gtgggctgca tcatggcaga agtttacacc ctcaggccac tcttccctgg agccagtgaa
     1141 attgacacaa tattcaaaat ttgccaagtg ctggggacac caaaaaagac tgactggcct
     1201 gaaggctatc aactttcaag tgcaatgaac ttccgttggc cacagtgtgt acccaataac
     1261 ttaaagacct tgattcccaa tgctagcagt gaagcagtcc agctcctgag agacatgctt
     1321 cagtgggatc ccaagaaacg accaacagct agtcaggcac ttcgatatcc ttacttccaa
     1381 gttggacacc cactaggcag caccacacaa aaccttcagg attcagaaaa accacagaaa
     1441 ggcatcctgg aaaaggcagg cccacctcct tatattaagc cagtcccacc tgcccagcca
     1501 ccagccaagc cacacacacg aatttcttca cgacagcatc aagccagcca gccccctctg
     1561 catctcacgt acccctacaa agcagaggtc tccaggacag atcacccaag ccatctccag
     1621 gaggacaagc caagcccgtt gcttttccca tccctccaca acaagcatcc acagtcgaaa
     1681 atcacagctg gcctggagca caaaaatggt gagataaagc caaagagtag gagaaggtgg
     1741 ggtcttattt ccaggtcaac aaaggattca gatgattggg ctgacttgga tgacttggat
     1801 ttcagtccat ccctcagcag gattgacctg aaaaacaaga aaagacagag tgatgacact
     1861 ctctgcaggt ttgagagtgt tttggacctg aagccctctg agcctgtggg cacaggaaac
     1921 agtgccccca cccagacgtc atatcagcgg cgagacacgc ccaccctgag atctgcagcc
     1981 aagcagcact atttgaagca ctctcgatac ttgcctggga tcagtataag aaatggcata
     2041 ctctcgaatc caggcaagga atttattcca cctaatccat ggtctagttc tggcttgtct
     2101 ggaaaatctt cagggacaat gtcagtaatc agcaaagtaa attcagttgg ttccagctct
     2161 acaagttcta gtggactgac tggaaactat gtcccttcct ttctgaaaaa agaaatcggt
     2221 tctgctatgc agagggtaca cctagcacct attccagacc cttcccctgg ttattcctcc
     2281 ctgaaggcca tgagacctca tcctgggcga ccattcttcc acacccagcc tagaagcact
     2341 cctgggttga taccacggcc tccagccgcc cagccagtgc atggccggac agactgggct
     2401 tccaagtacg catctcggcg atgactgtct gccttggtga tgaatctctt cctagggaga
     2461 agcaggatac tttccctcag ctgactggtg ttctacctgc aagatgtgca gagggcataa
     2521 aagcaaatca acactttata gttattcttc tgaactaaga catgtcaata ttctttttta
     2581 aagttttttt ttaaaatatt gatttgaatg cagtaggctt ttttgtataa aattatttta
     2641 ttctaaaact gggtcccatt attttcttaa acaacagcat tttgtatata tggattatgt
     2701 tttagcattt tatacagtca actttgtaat gaacttttta aaaattaatt gattttcctt
     2761 tggggttcca gataatattt tctacagatt ttgaaaaatg taataatatt aatgcagtat
     2821 tgcaacaggg gtgcaattta aggctatgtg atagagggtt atttactcag tgtgtgcaga
     2881 tatttatgaa gtggtgaaat ttcaagtgtg gctcactagg tacttcaggc cttcttggac
     2941 tgttgttaga aaagtgatcc tctgcttttc ttagtaggtc attggtttga tttttggata
     3001 ccactctgct gttctaaaag gactattata ttatataatt cactttgttt tacttttgtt
     3061 ccccagatga aagaactcta agtaaataca ttttaaaaaa tttttctgac accctttaat
     3121 gtggttgcag atctcagatg aaaccaagct taattatact atgccattat attctaattt
     3181 attccatttt tgaaatcaag ttgtatgtgt accaataaaa gagatttctg cttcaaaagg
     3241 ctctcaacat gaaggttaac acagtcaatc aaacttacat tcctgccaag atgcatggcc
     3301 aaaaaactaa gtatcaaagc agcagaaggt ttttgattat agtaactgag atggaatttt
     3361 gtgcctagct cagttctcca gatctggcta ggagcagtca atgactaatg ttctgtccta
     3421 gccaaattct caggacaatt tggggagcag aaagagttat ggcagaggtt ccactcatct
     3481 acaaagtcac agtcacatgc cacatttgat ctcctaaccc tggtgtagtt tctttcaaga
     3541 gtgagaactt tatttgttgg gcagaggctg ttccattgag aggaatgttt acagcagttt
     3601 caaaaatgac aaagtcagtt tggagacaga aaaagacaaa aggtccagtc tcatccatct
     3661 ctatatggta catttgcctc acttatggtt gccttaaagg caagagggaa ggtcaccatc
     3721 agtgaacgca atgcaatctc aacagtgtat tgattcatat tctcctaggg ctcaaactac
     3781 tctctattgg ttccaggata atgacaaatt gaaccatatg taagtaatct tttatttttt
     3841 attttttttt tgagacagag tctcactctg tcacccaggc tggagtgcag tggcgcgatc
     3901 ttagctctct gcaacctctg cctcccaggt tcaagcctcc tgagtaactg ggactacagg
     3961 cgcccgccac cacgcccagc taattttttg tatttttagt agagacgggg tttcactgtg
     4021 ttagccagga cggcctcgat ctcctgacct cgtgatccac cctcctccac ctcccaaagt
     4081 actgggatta caggcatgag ccactgcacc cagccaagtg atcattttta taggttaaaa
     4141 tgataggtga aatgaatata gacactttca tatggttcaa cctaatgact tggtaaatta
     4201 ttgccttggt gtattaataa tatgttgcat tctgaacaaa taaccatggc ttccaaaggg
     4261 ccctaaccta aaatcggaga gtaatttatg ctttggagaa tttgactcaa atatatactt
     4321 gaccaagcac catgatccct aggggcatga gaaaagcaca taatggatgt ggatgtgata
     4381 ggtggtcttt tcctgttaac aagctggcag caaagcttca gaaaatatat atgcaagcac
     4441 aacttgaagc tgaattcatt tctgtattat attctcaact cgttatctaa agcatcagaa
     4501 catgtgtttt cagagatgag tcctttacta taaggttaat atttattttc attttctgta
     4561 ttatatatga aaagtaaatt aatgtgaaac ctggcccagc ttgctggaaa gcaggtttta
     4621 aattgtaaat attccttaga ggagcaaatg gattgtttaa taccatagtc tcagtaatct
     4681 agcttatata aggtcattac attttttaac tgaaaaacct agttacctga ttattgcaca
     4741 ttataaaatt gtttttctaa tactttatag ggcccaactt cagaaaatac ttcgcttttt
     4801 tctttttatg ctttcgtttg tttaccagca agcaacttcc ctggggaagc caaacacata
     4861 ttcataaaaa aaatcaagta gctgatgtgc agttgagaaa actagaggac tgaaaaaaca
     4921 aattttaact agcaaatgct gtgaattact cttcctcccc ttctctgaaa tgggtaaagg
     4981 acaaattgtg taaaaaaacc tatgcactat agaagggaat agtaaccatt tcttttgtct
     5041 ctctgtttct gttctgactg agaacctgca gccatttctt gttacatgaa aacaaaatgc
     5101 tacttgttac ctctattttt tgttactata caattatgaa atgtaatgta agacaccaac
     5161 agaaatgata tacctgtaac tgtacctatc aggactatac ctcatttaca gtcagaaagc
     5221 ttactgggat gtcaggaaat gatacagggt tggttctcat ttcgtgccga aatgagacag
     5281 aaattcagtg acgaaggtgc gttgtagggg tattgatgtg ccccaggtag tgccagcaga
     5341 gtagggaaaa ctgcatttgc ataaaaacta ctcttgacat gattgttcat tttacaaaaa
     5401 aattccatta attaccaagc cctcacccag cccatgtgtg ataggattta tgtaggaaga
     5461 aacttgattt tcaaataatt ttttaaatgt atctcttgcc taaaggacta tatacatcta
     5521 ataaagtaac actgtgtcat cttctggagt tatcaaaaat tgtatacaat caagacaaca
     5581 caagaattat tttatttttg agtgcaaata caggtactgt tggagttgat gggcaccatg
     5641 ctttctcatg aagtagcatt tccctaccat caagccattg ttttgtgcca ttcaggagag
     5701 gaaaaaaagg aatttatgct gtacatttca gttcagtgta tgaccaaaag caatatgttt
     5761 ataagaagat gtttgacata ctaattattt tatatcattt aaaccatact gtagcaacat
     5821 aatatatgga gctaatttgt agaattattt ttacgatttc caaacaaatg tactgtactg
     5881 ttatataatt tattgtgagg accttctcat ggaagccatt aggaaaacaa actagaggta
     5941 aatatcacat taatctgtat tatcaatttc tcatagacac tgtgctaatg tgaattttaa
     6001 atgacctgca tcaagtcttc tgatctcaga taactcagta cagatagcaa ttagtcagct
     6061 gatttgatta caatggagta accgacaata tatttattta taaagcacat attcataata
     6121 acgagaagaa ttcagaaaac cacttaagca agacccttct gaaataaaaa atgttgcttt
     6181 ttaaatagtt tgtcctaagg tgtttaaaac atgtcaacct tatgtaagga aaaatttcct
     6241 ggtccaaata aagttgaagt ttaagaaaaa
//
