LOCUS       NM_032231                919 bp    mRNA    linear   PRI 10-FEB-2025
DEFINITION  Homo sapiens cytosolic iron-sulfur assembly component 2A (CIAO2A),
            transcript variant 1, mRNA.
ACCESSION   NM_032231
VERSION     NM_032231.7
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Zhao,N., He,M., Chen,W., Jin,P., Cao,L., Deng,J., Cheng,X. and
            Wang,L.
  TITLE     FAM96A suppresses epithelial-mesenchymal transition and tumor
            metastasis by inhibiting TGFbeta1 signals
  JOURNAL   Life Sci 301, 120607 (2022)
   PUBMED   35513087
  REMARK    GeneRIF: FAM96A suppresses epithelial-mesenchymal transition and
            tumor metastasis by inhibiting TGFbeta1 signals.
REFERENCE   2  (bases 1 to 919)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 919)
  AUTHORS   Maione,V., Grifagni,D., Torricella,F., Cantini,F. and Banci,L.
  TITLE     CIAO3 protein forms a stable ternary complex with two key players
            of the human cytosolic iron-sulfur cluster assembly machinery
  JOURNAL   J Biol Inorg Chem 25 (3), 501-508 (2020)
   PUBMED   32222833
REFERENCE   4  (bases 1 to 919)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   5  (bases 1 to 919)
  AUTHORS   Zhang,M.Y. and Wang,J.P.
  TITLE     A multi-target protein of hTERTR-FAM96A presents significant
            anticancer potent in the treatment of hepatocellular carcinoma
  JOURNAL   Tumour Biol 39 (4), 1010428317698341 (2017)
   PUBMED   28443470
  REMARK    GeneRIF: our data suggest that hTERTR-FAM96A may serve as an
            efficient anti-tumor agent for the treatment of hepatocellular
            carcinoma.
REFERENCE   6  (bases 1 to 919)
  AUTHORS   Schwamb,B., Pick,R., Fernandez,S.B., Volp,K., Heering,J.,
            Dotsch,V., Bosser,S., Jung,J., Beinoraviciute-Kellner,R.,
            Wesely,J., Zornig,I., Hammerschmidt,M., Nowak,M., Penzel,R.,
            Zatloukal,K., Joos,S., Rieker,R.J., Agaimy,A., Soder,S.,
            Reid-Lombardo,K.M., Kendrick,M.L., Bardsley,M.R., Hayashi,Y.,
            Asuzu,D.T., Syed,S.A., Ordog,T. and Zornig,M.
  TITLE     FAM96A is a novel pro-apoptotic tumor suppressor in
            gastrointestinal stromal tumors
  JOURNAL   Int J Cancer 137 (6), 1318-1329 (2015)
   PUBMED   25716227
  REMARK    GeneRIF: FAM96A expression was much reduced in tumorigenic ICC-SCs.
REFERENCE   7  (bases 1 to 919)
  AUTHORS   Marcon,E., Ni,Z., Pu,S., Turinsky,A.L., Trimble,S.S., Olsen,J.B.,
            Silverman-Gavrila,R., Silverman-Gavrila,L., Phanse,S., Guo,H.,
            Zhong,G., Guo,X., Young,P., Bailey,S., Roudeva,D., Zhao,D.,
            Hewel,J., Li,J., Graslund,S., Paduch,M., Kossiakoff,A.A.,
            Lupien,M., Emili,A., Wodak,S.J. and Greenblatt,J.
  TITLE     Human-chromatin-related protein interactions identify a demethylase
            complex required for chromosome segregation
  JOURNAL   Cell Rep 8 (1), 297-310 (2014)
   PUBMED   24981860
REFERENCE   8  (bases 1 to 919)
  AUTHORS   Stehling,O., Mascarenhas,J., Vashisht,A.A., Sheftel,A.D.,
            Niggemeyer,B., Rosser,R., Pierik,A.J., Wohlschlegel,J.A. and
            Lill,R.
  TITLE     Human CIA2A-FAM96A and CIA2B-FAM96B integrate iron homeostasis and
            maturation of different subsets of cytosolic-nuclear iron-sulfur
            proteins
  JOURNAL   Cell Metab 18 (2), 187-198 (2013)
   PUBMED   23891004
  REMARK    GeneRIF: CIA2B-CIA1-MMS19 and CIA2A-CIA1 assist different branches
            of Fe/S protein assembly and intimately link this process to
            cellular iron regulation via IRP1 Fe/S cluster maturation and IRP2
            stabilization.
            Erratum:[Cell Metab. 2018 Jan 9;27(1):263. doi:
            10.1016/j.cmet.2017.12.009.. PMID: 29320706]
REFERENCE   9  (bases 1 to 919)
  AUTHORS   Chen,K.E., Richards,A.A., Ariffin,J.K., Ross,I.L., Sweet,M.J.,
            Kellie,S., Kobe,B. and Martin,J.L.
  TITLE     The mammalian DUF59 protein Fam96a forms two distinct types of
            domain-swapped dimer
  JOURNAL   Acta Crystallogr D Biol Crystallogr 68 (Pt 6), 637-648 (2012)
   PUBMED   22683786
  REMARK    GeneRIF: putative metal-binding site of bacterial DUF59 proteins is
            not conserved in Fam96a, but Fam96a interacts tightly in vitro with
            Ciao1, the cytosolic iron-assembly protein
REFERENCE   10 (bases 1 to 919)
  AUTHORS   Ozyildirim,A.M., Wistow,G.J., Gao,J., Wang,J., Dickinson,D.P.,
            Frierson,H.F. Jr. and Laurie,G.W.
  TITLE     The lacrimal gland transcriptome is an unusually rich source of
            rare and poorly characterized gene transcripts
  JOURNAL   Invest Ophthalmol Vis Sci 46 (5), 1572-1580 (2005)
   PUBMED   15851553
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC021541.7.
            
            On Aug 31, 2019 this sequence version replaced NM_032231.6.
            
            Transcript Variant: This variant (1) represents the predominant
            transcript and encodes the longer isoform (a).
            
            ##Evidence-Data-START##
            Transcript exon combination :: BM806638.1, BQ014706.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN03267769 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000300030.8/ ENSP00000300030.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-194               AC021541.7         185262-185455       c
            195-359             AC021541.7         180304-180468       c
            360-409             AC021541.7         172719-172768       c
            410-455             AC021541.7         167109-167154       c
            456-919             AC021541.7         164182-164645       c
FEATURES             Location/Qualifiers
     source          1..919
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="15"
                     /map="15q22.31"
     gene            1..919
                     /gene="CIAO2A"
                     /gene_synonym="CIA2A; FAM96A"
                     /note="cytosolic iron-sulfur assembly component 2A"
                     /db_xref="GeneID:84191"
                     /db_xref="HGNC:HGNC:26235"
                     /db_xref="MIM:618382"
     exon            1..194
                     /gene="CIAO2A"
                     /gene_synonym="CIA2A; FAM96A"
                     /inference="alignment:Splign:2.1.0"
     CDS             71..553
                     /gene="CIAO2A"
                     /gene_synonym="CIA2A; FAM96A"
                     /note="isoform a is encoded by transcript variant 1; MIP18
                     family protein FAM96A; family with sequence similarity 96
                     member A"
                     /codon_start=1
                     /product="cytosolic iron-sulfur assembly component 2A
                     isoform a"
                     /protein_id="NP_115607.1"
                     /db_xref="CCDS:CCDS10189.1"
                     /db_xref="GeneID:84191"
                     /db_xref="HGNC:HGNC:26235"
                     /db_xref="MIM:618382"
                     /translation="MQRVSGLLSWTLSRVLWLSGLSEPGAARQPRIMEEKALEVYDLI
                     RTIRDPEKPNTLEELEVVSESCVEVQEINEEEYLVIIRFTPTVPHCSLATLIGLCLRV
                     KLQRCLPFKHKLEIYISEGTHSTEEDINKQINDKERVAAAMENPNLREIVEQCVLEPD
                     "
     exon            195..359
                     /gene="CIAO2A"
                     /gene_synonym="CIA2A; FAM96A"
                     /inference="alignment:Splign:2.1.0"
     exon            360..409
                     /gene="CIAO2A"
                     /gene_synonym="CIA2A; FAM96A"
                     /inference="alignment:Splign:2.1.0"
     exon            410..455
                     /gene="CIAO2A"
                     /gene_synonym="CIA2A; FAM96A"
                     /inference="alignment:Splign:2.1.0"
     exon            456..919
                     /gene="CIAO2A"
                     /gene_synonym="CIA2A; FAM96A"
                     /inference="alignment:Splign:2.1.0"
     regulatory      897..902
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CIAO2A"
                     /gene_synonym="CIA2A; FAM96A"
                     /note="hexamer: AATAAA"
     polyA_site      919
                     /gene="CIAO2A"
                     /gene_synonym="CIA2A; FAM96A"
                     /note="major polyA site"
ORIGIN      
        1 gatcaatccc gaggctgctg agagacggtg gcgcgattgg gacagtcgcc agggatggct
       61 gagcgtgaag atgcagcggg tgtccgggct gctctcctgg acgctgagca gagtcctgtg
      121 gctctccggc ctctctgagc cgggagctgc ccggcagccc cggatcatgg aagagaaagc
      181 gctagaagtt tatgatttga ttagaactat ccgggaccca gaaaagccca atactttaga
      241 agaactggaa gtggtctcgg aaagttgtgt ggaagttcag gagataaatg aagaagaata
      301 tctggttatt atcaggttca cgccaacagt acctcattgc tctttggcga ctcttattgg
      361 gctgtgctta agagtaaaac ttcagcgatg tttaccattt aaacataagt tggaaatcta
      421 catttctgaa ggaacccact caacagaaga agacatcaat aagcagataa atgacaaaga
      481 gcgagtggca gctgcaatgg aaaaccccaa cttacgggaa attgtggaac agtgtgtcct
      541 tgaacctgac tgatagctgt tttaagagcc actggcctgt aattgtttga tatatttgtt
      601 taaactcttt gtataatgtc agagactcat gtttaataca taggtgattt gtacctcaga
      661 gtatttttta aaggattctt tccaagcgag atttaattat aaggtagtac ctaatttgtt
      721 caatgtataa cattctcagg atttgtaaca cttaaatgat cagacagaat aatattttct
      781 agttattatg tgcaagatga gttgctattt ttctgatgct cattctgata caactatttt
      841 tcgtgtcaaa tatctactgt gcccaaatgt actcaattta aatcattact ctgtaaaata
      901 aataagcaga tgattctta
//
