LOCUS       NM_001289108            3332 bp    mRNA    linear   PRI 12-FEB-2025
DEFINITION  Homo sapiens cytosolic iron-sulfur assembly component 2A (CIAO2A),
            transcript variant 3, mRNA.
ACCESSION   NM_001289108
VERSION     NM_001289108.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3332)
  AUTHORS   Zhao,N., He,M., Chen,W., Jin,P., Cao,L., Deng,J., Cheng,X. and
            Wang,L.
  TITLE     FAM96A suppresses epithelial-mesenchymal transition and tumor
            metastasis by inhibiting TGFbeta1 signals
  JOURNAL   Life Sci 301, 120607 (2022)
   PUBMED   35513087
  REMARK    GeneRIF: FAM96A suppresses epithelial-mesenchymal transition and
            tumor metastasis by inhibiting TGFbeta1 signals.
REFERENCE   2  (bases 1 to 3332)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 3332)
  AUTHORS   Maione,V., Grifagni,D., Torricella,F., Cantini,F. and Banci,L.
  TITLE     CIAO3 protein forms a stable ternary complex with two key players
            of the human cytosolic iron-sulfur cluster assembly machinery
  JOURNAL   J Biol Inorg Chem 25 (3), 501-508 (2020)
   PUBMED   32222833
REFERENCE   4  (bases 1 to 3332)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   5  (bases 1 to 3332)
  AUTHORS   Zhang,M.Y. and Wang,J.P.
  TITLE     A multi-target protein of hTERTR-FAM96A presents significant
            anticancer potent in the treatment of hepatocellular carcinoma
  JOURNAL   Tumour Biol 39 (4), 1010428317698341 (2017)
   PUBMED   28443470
  REMARK    GeneRIF: our data suggest that hTERTR-FAM96A may serve as an
            efficient anti-tumor agent for the treatment of hepatocellular
            carcinoma.
REFERENCE   6  (bases 1 to 3332)
  AUTHORS   Schwamb,B., Pick,R., Fernandez,S.B., Volp,K., Heering,J.,
            Dotsch,V., Bosser,S., Jung,J., Beinoraviciute-Kellner,R.,
            Wesely,J., Zornig,I., Hammerschmidt,M., Nowak,M., Penzel,R.,
            Zatloukal,K., Joos,S., Rieker,R.J., Agaimy,A., Soder,S.,
            Reid-Lombardo,K.M., Kendrick,M.L., Bardsley,M.R., Hayashi,Y.,
            Asuzu,D.T., Syed,S.A., Ordog,T. and Zornig,M.
  TITLE     FAM96A is a novel pro-apoptotic tumor suppressor in
            gastrointestinal stromal tumors
  JOURNAL   Int J Cancer 137 (6), 1318-1329 (2015)
   PUBMED   25716227
  REMARK    GeneRIF: FAM96A expression was much reduced in tumorigenic ICC-SCs.
REFERENCE   7  (bases 1 to 3332)
  AUTHORS   Marcon,E., Ni,Z., Pu,S., Turinsky,A.L., Trimble,S.S., Olsen,J.B.,
            Silverman-Gavrila,R., Silverman-Gavrila,L., Phanse,S., Guo,H.,
            Zhong,G., Guo,X., Young,P., Bailey,S., Roudeva,D., Zhao,D.,
            Hewel,J., Li,J., Graslund,S., Paduch,M., Kossiakoff,A.A.,
            Lupien,M., Emili,A., Wodak,S.J. and Greenblatt,J.
  TITLE     Human-chromatin-related protein interactions identify a demethylase
            complex required for chromosome segregation
  JOURNAL   Cell Rep 8 (1), 297-310 (2014)
   PUBMED   24981860
REFERENCE   8  (bases 1 to 3332)
  AUTHORS   Stehling,O., Mascarenhas,J., Vashisht,A.A., Sheftel,A.D.,
            Niggemeyer,B., Rosser,R., Pierik,A.J., Wohlschlegel,J.A. and
            Lill,R.
  TITLE     Human CIA2A-FAM96A and CIA2B-FAM96B integrate iron homeostasis and
            maturation of different subsets of cytosolic-nuclear iron-sulfur
            proteins
  JOURNAL   Cell Metab 18 (2), 187-198 (2013)
   PUBMED   23891004
  REMARK    GeneRIF: CIA2B-CIA1-MMS19 and CIA2A-CIA1 assist different branches
            of Fe/S protein assembly and intimately link this process to
            cellular iron regulation via IRP1 Fe/S cluster maturation and IRP2
            stabilization.
            Erratum:[Cell Metab. 2018 Jan 9;27(1):263. doi:
            10.1016/j.cmet.2017.12.009.. PMID: 29320706]
REFERENCE   9  (bases 1 to 3332)
  AUTHORS   Chen,K.E., Richards,A.A., Ariffin,J.K., Ross,I.L., Sweet,M.J.,
            Kellie,S., Kobe,B. and Martin,J.L.
  TITLE     The mammalian DUF59 protein Fam96a forms two distinct types of
            domain-swapped dimer
  JOURNAL   Acta Crystallogr D Biol Crystallogr 68 (Pt 6), 637-648 (2012)
   PUBMED   22683786
  REMARK    GeneRIF: putative metal-binding site of bacterial DUF59 proteins is
            not conserved in Fam96a, but Fam96a interacts tightly in vitro with
            Ciao1, the cytosolic iron-assembly protein
REFERENCE   10 (bases 1 to 3332)
  AUTHORS   Ozyildirim,A.M., Wistow,G.J., Gao,J., Wang,J., Dickinson,D.P.,
            Frierson,H.F. Jr. and Laurie,G.W.
  TITLE     The lacrimal gland transcriptome is an unusually rich source of
            rare and poorly characterized gene transcripts
  JOURNAL   Invest Ophthalmol Vis Sci 46 (5), 1572-1580 (2005)
   PUBMED   15851553
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC021541.7.
            
            On Aug 13, 2020 this sequence version replaced NM_001289108.1.
            
            Transcript Variant: This variant (3) lacks an internal coding exon
            that causes a frame-shift and differs at the 3' end compared to
            variant 1. The resulting shorter isoform (b) has a distinct
            C-terminus compared to isoform a. Variants 2 and 3 encode the same
            isoform.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038194.2543069.1,
                                           SRR14038193.3801022.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN03267769 [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-194               AC021541.7         185262-185455       c
            195-359             AC021541.7         180304-180468       c
            360-3332            AC021541.7         164182-167154       c
FEATURES             Location/Qualifiers
     source          1..3332
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="15"
                     /map="15q22.31"
     gene            1..3332
                     /gene="CIAO2A"
                     /gene_synonym="CIA2A; FAM96A"
                     /note="cytosolic iron-sulfur assembly component 2A"
                     /db_xref="GeneID:84191"
                     /db_xref="HGNC:HGNC:26235"
                     /db_xref="MIM:618382"
     exon            1..194
                     /gene="CIAO2A"
                     /gene_synonym="CIA2A; FAM96A"
                     /inference="alignment:Splign:2.1.0"
     CDS             71..379
                     /gene="CIAO2A"
                     /gene_synonym="CIA2A; FAM96A"
                     /note="isoform b is encoded by transcript variant 3; MIP18
                     family protein FAM96A; family with sequence similarity 96
                     member A"
                     /codon_start=1
                     /product="cytosolic iron-sulfur assembly component 2A
                     isoform b"
                     /protein_id="NP_001276037.1"
                     /db_xref="CCDS:CCDS45278.1"
                     /db_xref="GeneID:84191"
                     /db_xref="HGNC:HGNC:26235"
                     /db_xref="MIM:618382"
                     /translation="MQRVSGLLSWTLSRVLWLSGLSEPGAARQPRIMEEKALEVYDLI
                     RTIRDPEKPNTLEELEVVSESCVEVQEINEEEYLVIIRFTPTVPHCSLATLIVGNLHF
                     "
     exon            195..359
                     /gene="CIAO2A"
                     /gene_synonym="CIA2A; FAM96A"
                     /inference="alignment:Splign:2.1.0"
     exon            360..3332
                     /gene="CIAO2A"
                     /gene_synonym="CIA2A; FAM96A"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3310..3315
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CIAO2A"
                     /gene_synonym="CIA2A; FAM96A"
                     /note="hexamer: AATAAA"
     polyA_site      3332
                     /gene="CIAO2A"
                     /gene_synonym="CIA2A; FAM96A"
                     /note="major polyA site"
ORIGIN      
        1 gatcaatccc gaggctgctg agagacggtg gcgcgattgg gacagtcgcc agggatggct
       61 gagcgtgaag atgcagcggg tgtccgggct gctctcctgg acgctgagca gagtcctgtg
      121 gctctccggc ctctctgagc cgggagctgc ccggcagccc cggatcatgg aagagaaagc
      181 gctagaagtt tatgatttga ttagaactat ccgggaccca gaaaagccca atactttaga
      241 agaactggaa gtggtctcgg aaagttgtgt ggaagttcag gagataaatg aagaagaata
      301 tctggttatt atcaggttca cgccaacagt acctcattgc tctttggcga ctcttattgt
      361 tggaaatcta catttctgaa ggaacccact caacagaaga agacagtaag tgaatgttga
      421 aacataattg aaaaacaact tcagatagta ttttatcttg gatactggat tcttctgcca
      481 gccaagaatc tactgggtta ctttcaaaaa agtgcacaat tggttattgg ctcttccttc
      541 ctaggtgagg aaacctaggc atttcaaaga agtaattttt gcagtggact ccgagtgcac
      601 tagagcccac ggatgttgac tggtttaggc cagtggctac acatctgtaa agaatatcca
      661 gtgctgggga ccttgactta tgagctctaa gtaggcttct tttctttggt gacaggatag
      721 agagctgtgt acttgaaatt gaaggtctag caagtagagc tttagtgaat cttggggtag
      781 gtgctgtctc tctgggttag tatattagaa tgggagtggg gaaccttttt tatatagagg
      841 acctctggcc tacagagaga aatcaatcag tagctactta aaaatagcag aagtggggtt
      901 tgggaaaagc aaaaaacaaa atgttctgta tgatccctgt gttacattaa aaacataatt
      961 ttaatgtttt taatctacaa atgatagagt gcaaataatg agaaatacat ttaaaaggca
     1021 tgttgtactt aagtttcatc actaagtaaa ttgagaaggt gggaaaaaat agcagaggaa
     1081 tggaagaaag agctaataga ggaagagcaa cagtgagata gataccaaga agactcagaa
     1141 aggcatacac agggctggag agagacacac aaaatctcct acataaaaga gagctataga
     1201 gagggaagcc tgctgcttcc agaggaacag cctatccagg aaacttttta ggtactgtca
     1261 tgaaaactgt taaaaaccat gccctattta tagttctaga catgtagatt attatactct
     1321 ttgtgggctg aatttcttta aaccccacga gctgagggct tctacctgta atttagaaaa
     1381 ataagagttt aggtacccta aatccactca ggttatcgtt ttgaaattcc ttgaatgata
     1441 ggattttaca gcagcaatag tattaagtct tatgagtctt gtttttcatt tagtaagtat
     1501 ttattaagtg ccttctatgt gtgggtacag ttcagccttt gagatacatt cctgcctttg
     1561 tggagcctac attgtagtag gaagaaacca aattgaaagt attttcgcag gaatatgtgt
     1621 atgtttgtgt gtcttctaca tgagacattt cagcggcagc ccagaaaact caatccacat
     1681 taagaatata gtcgtctctc ttttgaactc ttctattaag aatgctgtta gctaattaca
     1741 gaaaacataa tgggccaata cctttattat atgaagatcc attcaaacta attttaaaaa
     1801 aagatctagt agattaatag agacatgaac aggaaattca tgcaagaaga actaatatat
     1861 gcacatggaa aatgttctat tttcctatta gtcaaggaaa tgcaaattat gacaataata
     1921 ttgagccgtt ttccatctcc tgttttatgt atgtatatat gtgtatttgt ttttattaaa
     1981 taataccagc tattgcccgg gcatggtggt tcacacctgt aaccctagca ctttgggagg
     2041 ccaaggcgag agaatgcctt gagggaagga gtttgaggcc aggatttcaa gactagcctg
     2101 ggcaacatgg tgagaccctg tctctacgaa aatatttgaa aattagccag gtgtggtggt
     2161 tcatgcctgt gggaggctga ggtaggagga tctcttgagc ccaggagttg gaggttacag
     2221 tgagctatga ttgcaccact gcactccagc ctggatgaca gagcaggacc ctgtctcaaa
     2281 gaataataat aacaatgata ataataccag gtattgttgc caaactgata gctcatgtat
     2341 tggtgttgac aatataaact ggtgagtaat tttaggaagt aaatcaatga tatgaatcac
     2401 accataaaaa tattcatact cttaaaaata atcccatttt tgagaatatt tccaaaaaga
     2461 aggctatatg tttaaaggta ttcattgtag catagtcttc ataattactt tagaacactt
     2521 aagactttta ccatgttaaa taaccctgta gtttaaaata taggcttatg ggggaaaaaa
     2581 tagcaatttt caaaattata tttactctga atgtaactgt ataaaaatat gtgtatggac
     2641 aaagactgga aagaaaaaaa tagatgtggt taagtagtag aattctagta gatatttttc
     2701 tcttaagtct gccttctaga tgggaaatta cattatgagt aattagccaa aaacaggttt
     2761 aagttctcaa ggagttaaag tttttgtttc agcaggctaa taaaaatact gtctggtgct
     2821 gtatattgac acagttcttc tgctaacttt tgtctgtgta tttttcagtc aataagcaga
     2881 taaatgacaa agagcgagtg gcagctgcaa tggaaaaccc caacttacgg gaaattgtgg
     2941 aacagtgtgt ccttgaacct gactgatagc tgttttaaga gccactggcc tgtaattgtt
     3001 tgatatattt gtttaaactc tttgtataat gtcagagact catgtttaat acataggtga
     3061 tttgtacctc agagtatttt ttaaaggatt ctttccaagc gagatttaat tataaggtag
     3121 tacctaattt gttcaatgta taacattctc aggatttgta acacttaaat gatcagacag
     3181 aataatattt tctagttatt atgtgcaaga tgagttgcta tttttctgat gctcattctg
     3241 atacaactat ttttcgtgtc aaatatctac tgtgcccaaa tgtactcaat ttaaatcatt
     3301 actctgtaaa ataaataagc agatgattct ta
//
