LOCUS       NM_001278               3600 bp    mRNA    linear   PRI 12-JUN-2025
DEFINITION  Homo sapiens component of inhibitor of nuclear factor kappa B
            kinase complex (CHUK), transcript variant 1, mRNA.
ACCESSION   NM_001278
VERSION     NM_001278.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3600)
  AUTHORS   Chen,X., Wu,W., Jeong,J.H., Rokavec,M., Wei,R., Feng,S.,
            Schroth,W., Brauch,H., Zhong,S. and Luo,J.L.
  TITLE     Cytokines-activated nuclear IKKalpha-FAT10 pathway induces breast
            cancer tamoxifen-resistance
  JOURNAL   Sci China Life Sci 67 (7), 1413-1426 (2024)
   PUBMED   38565741
  REMARK    GeneRIF: Cytokines-activated nuclear IKKalpha-FAT10 pathway induces
            breast cancer tamoxifen-resistance.
REFERENCE   2  (bases 1 to 3600)
  AUTHORS   Chang,X., Guo,Y., Zhang,Q., Zheng,X., Cui,X., Hu,J., Zhang,Z.,
            Zhang,F. and Wang,X.
  TITLE     GRP78 recognizes EV-F 3D protein and activates NF-kappaB to repress
            virus replication by interacting with CHUK/IKBKB
  JOURNAL   J Virol 98 (6), e0026824 (2024)
   PUBMED   38775480
  REMARK    GeneRIF: GRP78 recognizes EV-F 3D protein and activates NF-kappaB
            to repress virus replication by interacting with CHUK/IKBKB.
REFERENCE   3  (bases 1 to 3600)
  AUTHORS   McIntosh,K., Khalaf,Y.H., Craig,R., West,C., McCulloch,A.,
            Waghmare,A., Lawson,C., Chan,E.Y.W., Mackay,S., Paul,A. and
            Plevin,R.
  TITLE     IL-1beta stimulates a novel, IKKalpha -dependent, NIK -independent
            activation of non-canonical NFkappaB signalling
  JOURNAL   Cell Signal 107, 110684 (2023)
   PUBMED   37080443
  REMARK    GeneRIF: IL-1beta stimulates a novel, IKKalpha -dependent, NIK
            -independent activation of non-canonical NFkappaB signalling.
REFERENCE   4  (bases 1 to 3600)
  AUTHORS   Yang,H., Espada,C.E., Phillips,S., Martinez,N., Kenney,A.D.,
            Yount,J.S., Xiong,Y. and Wu,L.
  TITLE     The host antiviral protein SAMHD1 suppresses NF-kappaB activation
            by interacting with the IKK complex during inflammatory responses
            and viral infection
  JOURNAL   J Biol Chem 299 (6), 104750 (2023)
   PUBMED   37100289
  REMARK    GeneRIF: The host antiviral protein SAMHD1 suppresses NF-kappaB
            activation by interacting with the IKK complex during inflammatory
            responses and viral infection.
REFERENCE   5  (bases 1 to 3600)
  AUTHORS   Stephenson,A.A., Taggart,D.J., Xu,G., Fowler,J.D., Wu,H. and Suo,Z.
  TITLE     The inhibitor of kappaB kinase beta (IKKbeta) phosphorylates
            IkappaBalpha twice in a single binding event through a sequential
            mechanism
  JOURNAL   J Biol Chem 299 (1), 102796 (2023)
   PUBMED   36528060
  REMARK    GeneRIF: The inhibitor of kappaB kinase beta (IKKbeta)
            phosphorylates IkappaBalpha twice in a single binding event through
            a sequential mechanism.
REFERENCE   6  (bases 1 to 3600)
  AUTHORS   Scherer,D.C., Brockman,J.A., Chen,Z., Maniatis,T. and Ballard,D.W.
  TITLE     Signal-induced degradation of I kappa B alpha requires
            site-specific ubiquitination
  JOURNAL   Proc Natl Acad Sci U S A 92 (24), 11259-11263 (1995)
   PUBMED   7479976
REFERENCE   7  (bases 1 to 3600)
  AUTHORS   Mock,B.A., Connelly,M.A., McBride,O.W., Kozak,C.A. and Marcu,K.B.
  TITLE     CHUK, a conserved helix-loop-helix ubiquitous kinase, maps to human
            chromosome 10 and mouse chromosome 19
  JOURNAL   Genomics 27 (2), 348-351 (1995)
   PUBMED   7558004
REFERENCE   8  (bases 1 to 3600)
  AUTHORS   Connelly,M.A. and Marcu,K.B.
  TITLE     CHUK, a new member of the helix-loop-helix and leucine zipper
            families of interacting proteins, contains a serine-threonine
            kinase catalytic domain
  JOURNAL   Cell Mol Biol Res 41 (6), 537-549 (1995)
   PUBMED   8777433
REFERENCE   9  (bases 1 to 3600)
  AUTHORS   Ganchi,P.A., Sun,S.C., Greene,W.C. and Ballard,D.W.
  TITLE     A novel NF-kappa B complex containing p65 homodimers: implications
            for transcriptional control at the level of subunit dimerization
  JOURNAL   Mol Cell Biol 13 (12), 7826-7835 (1993)
   PUBMED   8246997
REFERENCE   10 (bases 1 to 3600)
  AUTHORS   Ganchi,P.A., Sun,S.C., Greene,W.C. and Ballard,D.W.
  TITLE     I kappa B/MAD-3 masks the nuclear localization signal of NF-kappa B
            p65 and requires the transactivation domain to inhibit NF-kappa B
            p65 DNA binding
  JOURNAL   Mol Biol Cell 3 (12), 1339-1352 (1992)
   PUBMED   1493333
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA405845.1, AF080157.1,
            BM800404.1, BC034796.1 and AI829429.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_001278.4.
            
            Summary: This gene encodes a member of the serine/threonine protein
            kinase family. The encoded protein, a component of a
            cytokine-activated protein complex that is an inhibitor of the
            essential transcription factor NF-kappa-B complex, phosphorylates
            sites that trigger the degradation of the inhibitor via the
            ubiquination pathway, thereby activating the transcription factor.
            [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC092514.1, AF080157.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000370397.8/ ENSP00000359424.6
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-538               DA405845.1         16-553
            539-865             AF080157.1         570-896
            866-867             BM800404.1         65-66
            868-2677            AF080157.1         899-2708
            2678-2681           BC034796.1         1195-1198
            2682-3533           AF080157.1         2713-3564
            3534-3600           AI829429.1         15-81               c
FEATURES             Location/Qualifiers
     source          1..3600
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="10"
                     /map="10q24.31"
     gene            1..3600
                     /gene="CHUK"
                     /gene_synonym="BPS2; IKBKA; IKK-1; IKK-alpha; IKK1; IKKA;
                     NFKBIKA; TCF16"
                     /note="component of inhibitor of nuclear factor kappa B
                     kinase complex"
                     /db_xref="GeneID:1147"
                     /db_xref="HGNC:HGNC:1974"
                     /db_xref="MIM:600664"
     exon            1..169
                     /gene="CHUK"
                     /gene_synonym="BPS2; IKBKA; IKK-1; IKK-alpha; IKK1; IKKA;
                     NFKBIKA; TCF16"
                     /inference="alignment:Splign:2.1.0"
     CDS             65..2302
                     /gene="CHUK"
                     /gene_synonym="BPS2; IKBKA; IKK-1; IKK-alpha; IKK1; IKKA;
                     NFKBIKA; TCF16"
                     /EC_number="2.7.11.10"
                     /note="isoform 1 is encoded by transcript variant 1;
                     I-kappa-B kinase-alpha; I-kappa-B kinase 1; Nuclear factor
                     NFkappaB inhibitor kinase alpha; IKK-a kinase; IkB kinase
                     alpha subunit; inhibitor of nuclear factor kappa-B kinase
                     subunit alpha; conserved helix-loop-helix ubiquitous
                     kinase; transcription factor 16"
                     /codon_start=1
                     /product="inhibitor of nuclear factor kappa-B kinase
                     subunit alpha isoform 1"
                     /protein_id="NP_001269.3"
                     /db_xref="CCDS:CCDS7488.1"
                     /db_xref="GeneID:1147"
                     /db_xref="HGNC:HGNC:1974"
                     /db_xref="MIM:600664"
                     /translation="MERPPGLRPGAGGPWEMRERLGTGGFGNVCLYQHRELDLKIAIK
                     SCRLELSTKNRERWCHEIQIMKKLNHANVVKACDVPEELNILIHDVPLLAMEYCSGGD
                     LRKLLNKPENCCGLKESQILSLLSDIGSGIRYLHENKIIHRDLKPENIVLQDVGGKII
                     HKIIDLGYAKDVDQGSLCTSFVGTLQYLAPELFENKPYTATVDYWSFGTMVFECIAGY
                     RPFLHHLQPFTWHEKIKKKDPKCIFACEEMSGEVRFSSHLPQPNSLCSLVVEPMENWL
                     QLMLNWDPQQRGGPVDLTLKQPRCFVLMDHILNLKIVHILNMTSAKIISFLLPPDESL
                     HSLQSRIERETGINTGSQELLSETGISLDPRKPASQCVLDGVRGCDSYMVYLFDKSKT
                     VYEGPFASRSLSDCVNYIVQDSKIQLPIIQLRKVWAEAVHYVSGLKEDYSRLFQGQRA
                     AMLSLLRYNANLTKMKNTLISASQQLKAKLEFFHKSIQLDLERYSEQMTYGISSEKML
                     KAWKEMEEKAIHYAEVGVIGYLEDQIMSLHAEIMELQKSPYGRRQGDLMESLEQRAID
                     LYKQLKHRPSDHSYSDSTEMVKIIVHTVQSQDRVLKELFGHLSKLLGCKQKIIDLLPK
                     VEVALSNIKEADNTVMFMQGKRQKEIWHLLKIACTQSSARSLVGSSLEGAVTPQTSAW
                     LPPTSAEHDHSLSCVVTPQDGETSAQMIEENLNCLGHLSTIIHEANEEQGNSMMNLDW
                     SWLTE"
     exon            170..264
                     /gene="CHUK"
                     /gene_synonym="BPS2; IKBKA; IKK-1; IKK-alpha; IKK1; IKKA;
                     NFKBIKA; TCF16"
                     /inference="alignment:Splign:2.1.0"
     exon            265..379
                     /gene="CHUK"
                     /gene_synonym="BPS2; IKBKA; IKK-1; IKK-alpha; IKK1; IKKA;
                     NFKBIKA; TCF16"
                     /inference="alignment:Splign:2.1.0"
     exon            380..449
                     /gene="CHUK"
                     /gene_synonym="BPS2; IKBKA; IKK-1; IKK-alpha; IKK1; IKKA;
                     NFKBIKA; TCF16"
                     /inference="alignment:Splign:2.1.0"
     exon            450..538
                     /gene="CHUK"
                     /gene_synonym="BPS2; IKBKA; IKK-1; IKK-alpha; IKK1; IKKA;
                     NFKBIKA; TCF16"
                     /inference="alignment:Splign:2.1.0"
     exon            539..628
                     /gene="CHUK"
                     /gene_synonym="BPS2; IKBKA; IKK-1; IKK-alpha; IKK1; IKKA;
                     NFKBIKA; TCF16"
                     /inference="alignment:Splign:2.1.0"
     exon            629..753
                     /gene="CHUK"
                     /gene_synonym="BPS2; IKBKA; IKK-1; IKK-alpha; IKK1; IKKA;
                     NFKBIKA; TCF16"
                     /inference="alignment:Splign:2.1.0"
     exon            754..861
                     /gene="CHUK"
                     /gene_synonym="BPS2; IKBKA; IKK-1; IKK-alpha; IKK1; IKKA;
                     NFKBIKA; TCF16"
                     /inference="alignment:Splign:2.1.0"
     exon            862..997
                     /gene="CHUK"
                     /gene_synonym="BPS2; IKBKA; IKK-1; IKK-alpha; IKK1; IKKA;
                     NFKBIKA; TCF16"
                     /inference="alignment:Splign:2.1.0"
     exon            998..1192
                     /gene="CHUK"
                     /gene_synonym="BPS2; IKBKA; IKK-1; IKK-alpha; IKK1; IKKA;
                     NFKBIKA; TCF16"
                     /inference="alignment:Splign:2.1.0"
     exon            1193..1295
                     /gene="CHUK"
                     /gene_synonym="BPS2; IKBKA; IKK-1; IKK-alpha; IKK1; IKKA;
                     NFKBIKA; TCF16"
                     /inference="alignment:Splign:2.1.0"
     exon            1296..1419
                     /gene="CHUK"
                     /gene_synonym="BPS2; IKBKA; IKK-1; IKK-alpha; IKK1; IKKA;
                     NFKBIKA; TCF16"
                     /inference="alignment:Splign:2.1.0"
     exon            1420..1571
                     /gene="CHUK"
                     /gene_synonym="BPS2; IKBKA; IKK-1; IKK-alpha; IKK1; IKKA;
                     NFKBIKA; TCF16"
                     /inference="alignment:Splign:2.1.0"
     exon            1572..1633
                     /gene="CHUK"
                     /gene_synonym="BPS2; IKBKA; IKK-1; IKK-alpha; IKK1; IKKA;
                     NFKBIKA; TCF16"
                     /inference="alignment:Splign:2.1.0"
     exon            1634..1743
                     /gene="CHUK"
                     /gene_synonym="BPS2; IKBKA; IKK-1; IKK-alpha; IKK1; IKKA;
                     NFKBIKA; TCF16"
                     /inference="alignment:Splign:2.1.0"
     exon            1744..1793
                     /gene="CHUK"
                     /gene_synonym="BPS2; IKBKA; IKK-1; IKK-alpha; IKK1; IKKA;
                     NFKBIKA; TCF16"
                     /inference="alignment:Splign:2.1.0"
     exon            1794..1890
                     /gene="CHUK"
                     /gene_synonym="BPS2; IKBKA; IKK-1; IKK-alpha; IKK1; IKKA;
                     NFKBIKA; TCF16"
                     /inference="alignment:Splign:2.1.0"
     exon            1891..2038
                     /gene="CHUK"
                     /gene_synonym="BPS2; IKBKA; IKK-1; IKK-alpha; IKK1; IKKA;
                     NFKBIKA; TCF16"
                     /inference="alignment:Splign:2.1.0"
     exon            2039..2172
                     /gene="CHUK"
                     /gene_synonym="BPS2; IKBKA; IKK-1; IKK-alpha; IKK1; IKKA;
                     NFKBIKA; TCF16"
                     /inference="alignment:Splign:2.1.0"
     exon            2173..2272
                     /gene="CHUK"
                     /gene_synonym="BPS2; IKBKA; IKK-1; IKK-alpha; IKK1; IKKA;
                     NFKBIKA; TCF16"
                     /inference="alignment:Splign:2.1.0"
     exon            2273..3600
                     /gene="CHUK"
                     /gene_synonym="BPS2; IKBKA; IKK-1; IKK-alpha; IKK1; IKKA;
                     NFKBIKA; TCF16"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3514..3519
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CHUK"
                     /gene_synonym="BPS2; IKBKA; IKK-1; IKK-alpha; IKK1; IKKA;
                     NFKBIKA; TCF16"
                     /note="hexamer: ATTAAA"
     polyA_site      3533
                     /gene="CHUK"
                     /gene_synonym="BPS2; IKBKA; IKK-1; IKK-alpha; IKK1; IKKA;
                     NFKBIKA; TCF16"
                     /note="major polyA site"
     regulatory      3571..3576
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CHUK"
                     /gene_synonym="BPS2; IKBKA; IKK-1; IKK-alpha; IKK1; IKKA;
                     NFKBIKA; TCF16"
                     /note="hexamer: AATAAA"
     polyA_site      3600
                     /gene="CHUK"
                     /gene_synonym="BPS2; IKBKA; IKK-1; IKK-alpha; IKK1; IKKA;
                     NFKBIKA; TCF16"
ORIGIN      
        1 atcggcggcc cggaaccggc cttggaacaa ctgtggaacc tgaggccgct tgccctcccg
       61 ccccatggag cggcccccgg ggctgcggcc gggcgcgggc gggccctggg agatgcggga
      121 gcggctgggc accggcggct tcgggaacgt ctgtctgtac cagcatcggg aacttgatct
      181 caaaatagca attaagtctt gtcgcctaga gctaagtacc aaaaacagag aacgatggtg
      241 ccatgaaatc cagattatga agaagttgaa ccatgccaat gttgtaaagg cctgtgatgt
      301 tcctgaagaa ttgaatattt tgattcatga tgtgcctctt ctagcaatgg aatactgttc
      361 tggaggagat ctccgaaagc tgctcaacaa accagaaaat tgttgtggac ttaaagaaag
      421 ccagatactt tctttactaa gtgatatagg gtctgggatt cgatatttgc atgaaaacaa
      481 aattatacat cgagatctaa aacctgaaaa catagttctt caggatgttg gtggaaagat
      541 aatacataaa ataattgatc tgggatatgc caaagatgtt gatcaaggaa gtctgtgtac
      601 atcttttgtg ggaacactgc agtatctggc cccagagctc tttgagaata agccttacac
      661 agccactgtt gattattgga gctttgggac catggtattt gaatgtattg ctggatatag
      721 gccttttttg catcatctgc agccatttac ctggcatgag aagattaaga agaaggatcc
      781 aaagtgtata tttgcatgtg aagagatgtc aggagaagtt cggtttagta gccatttacc
      841 tcaaccaaat agcctttgta gtttagtagt agaacccatg gaaaactggc tacagttgat
      901 gttgaattgg gaccctcagc agagaggagg acctgttgac cttactttga agcagccaag
      961 atgttttgta ttaatggatc acattttgaa tttgaagata gtacacatcc taaatatgac
     1021 ttctgcaaag ataatttctt ttctgttacc acctgatgaa agtcttcatt cactacagtc
     1081 tcgtattgag cgtgaaactg gaataaatac tggttctcaa gaacttcttt cagagacagg
     1141 aatttctctg gatcctcgga aaccagcctc tcaatgtgtt ctagatggag ttagaggctg
     1201 tgatagctat atggtttatt tgtttgataa aagtaaaact gtatatgaag ggccatttgc
     1261 ttccagaagt ttatctgatt gtgtaaatta tattgtacag gacagcaaaa tacagcttcc
     1321 aattatacag ctgcgtaaag tgtgggctga agcagtgcac tatgtgtctg gactaaaaga
     1381 agactatagc aggctctttc agggacaaag ggcagcaatg ttaagtcttc ttagatataa
     1441 tgctaactta acaaaaatga agaacacttt gatctcagca tcacaacaac tgaaagctaa
     1501 attggagttt tttcacaaaa gcattcagct tgacttggag agatacagcg agcagatgac
     1561 gtatgggata tcttcagaaa aaatgctaaa agcatggaaa gaaatggaag aaaaggccat
     1621 ccactatgct gaggttggtg tcattggata cctggaggat cagattatgt ctttgcatgc
     1681 tgaaatcatg gagctacaga agagccccta tggaagacgt cagggagact tgatggaatc
     1741 tctggaacag cgtgccattg atctatataa gcagttaaaa cacagacctt cagatcactc
     1801 ctacagtgac agcacagaga tggtgaaaat cattgtgcac actgtgcaga gtcaggaccg
     1861 tgtgctcaag gagctgtttg gtcatttgag caagttgttg ggctgtaagc agaagattat
     1921 tgatctactc cctaaggtgg aagtggccct cagtaatatc aaagaagctg acaatactgt
     1981 catgttcatg cagggaaaaa ggcagaaaga aatatggcat ctccttaaaa ttgcctgtac
     2041 acagagttct gcccggtccc ttgtaggatc cagtctagaa ggtgcagtaa cccctcagac
     2101 atcagcatgg ctgcccccga cttcagcaga acatgatcat tctctgtcat gtgtggtaac
     2161 tcctcaagat ggggagactt cagcacaaat gatagaagaa aatttgaact gccttggcca
     2221 tttaagcact attattcatg aggcaaatga ggaacagggc aatagtatga tgaatcttga
     2281 ttggagttgg ttaacagaat gagttgtcac ttgttcactg tccccaaacc tatggaagtt
     2341 gttgctatac atgttggaaa tgtgtttttc ccccatgaaa ccattcttca gacatcagtc
     2401 aatggaagaa atggctatga acagaaacta catttctact atgatcagaa gaacatgatt
     2461 ttacaagtat aacagttttg agtaattcaa gcctctaaac agacaggaat ttagaaaaag
     2521 tcaatgtact tgtttgaata tttgttttaa taccacagct atttagaagc atcatcacga
     2581 cacatttgcc ttcagtcttg gtaaaacatt acttatttaa ctgattaaaa ataccttcta
     2641 tgtattagtg tcaactttta acttttgggc gtaagaccaa atgtagtttt gtatacagag
     2701 aagaaaacct caagtaatag gcattttaag taaaagtcta cctgtgtttt tttctaaaaa
     2761 ggctgctcac aagttctatt tcttgaagaa taaattctac ctccttgtgt tgcactgaac
     2821 aggttctctt cctggcatca taaggagttg gtgtaatcat tttaaattcc actgaaaatt
     2881 taacagtatc cccttctcat cgaagggatt gtgtatctgt gcttctaata ttagttggct
     2941 ttcataaatc atgttgttgt gtgtatatgt atttaagatg tacatttaat aatatcaaag
     3001 agaagatgcc tgttaattta taatgtattt gaaaattaca tgttttttca tttgtaaaaa
     3061 tgagtcattt gtttaaacaa tctttcatgt cttgtcatac aaatttataa aggtctgcac
     3121 tcctttatct gtaattgtaa ttccaaaatc caaaaagctc tgaaaacaag gtttccataa
     3181 gcttggtgac aaaattcatt tgcttgcaat ctaatctgaa ctgaccttga atctttttat
     3241 cccatttagt gtgaatattc ctttattttg ctgcttgatg atgagaggga gggctgctgc
     3301 cacagactgt ggtgagggct ggttaatgta gtatggtata tgcacaaaac tacttttcta
     3361 aaatctaaaa tttcataatt ctgaaacaac ttgccccaag ggtttcagag aaaggactgt
     3421 ggacctctat catctgctaa gtaatttaga agatattatt tgtcttaaaa aatgtgaaat
     3481 gcttttatat tctaatagtt tttcactttg tgtattaaat ggtttttaaa ttactttctt
     3541 gatctctatt cattataaaa atcagattat aataaaacag ttgaatatgg cttaggaaaa
//
