LOCUS       NM_175856               3857 bp    mRNA    linear   PRI 11-JUN-2024
DEFINITION  Homo sapiens chondroitin sulfate synthase 3 (CHSY3), mRNA.
ACCESSION   NM_175856 XM_373440
VERSION     NM_175856.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3857)
  AUTHORS   Wang,H., Zhang,J., Wei,Z., Chen,S., Zheng,J. and Li,Y.
  TITLE     The prognostic implications and tumor-promoting functions of CHSY3
            in gastric cancer
  JOURNAL   Front Immunol 15, 1364979 (2024)
   PUBMED   38812506
  REMARK    GeneRIF: The prognostic implications and tumor-promoting functions
            of CHSY3 in gastric cancer.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3857)
  AUTHORS   Huang,X., Liu,Y., Qian,C., Shen,Q., Wu,M., Zhu,B. and Feng,Y.
  TITLE     CHSY3 promotes proliferation and migration in gastric cancer and is
            associated with immune infiltration
  JOURNAL   J Transl Med 21 (1), 474 (2023)
   PUBMED   37461041
  REMARK    GeneRIF: CHSY3 promotes proliferation and migration in gastric
            cancer and is associated with immune infiltration.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3857)
  AUTHORS   Zhang,J., Chen,Z., Wang,B., Chen,J., Xiao,T., Zhang,J.V., Chen,S.
            and Fan,X.
  TITLE     Reduction of pl-CSA through ChSy-2 knockout inhibits tumorigenesis
            and metastasis of choriocarcinoma in JEG3 cells
  JOURNAL   Int J Med Sci 18 (1), 207-215 (2021)
   PUBMED   33390789
  REMARK    GeneRIF: Reduction of pl-CSA through ChSy-2 knockout inhibits
            tumorigenesis and metastasis of choriocarcinoma in JEG3 cells.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3857)
  AUTHORS   Wei,L., Cao,P., Xu,C., Zhong,H., Wang,X., Bai,M., Hu,B., Wang,R.,
            Liu,N., Tian,Y., Chen,H., Li,J. and Yuan,W.
  TITLE     Chondroitin synthase-3 regulates nucleus pulposus degeneration
            through actin-induced YAP signaling
  JOURNAL   FASEB J 34 (12), 16581-16600 (2020)
   PUBMED   33089528
  REMARK    GeneRIF: Chondroitin synthase-3 regulates nucleus pulposus
            degeneration through actin-induced YAP signaling.
REFERENCE   5  (bases 1 to 3857)
  AUTHORS   Kalathas,D., Theocharis,D.A., Bounias,D., Kyriakopoulou,D.,
            Papageorgakopoulou,N., Stavropoulos,M.S. and Vynios,D.H.
  TITLE     Chondroitin synthases I, II, III and chondroitin sulfate
            glucuronyltransferase expression in colorectal cancer
  JOURNAL   Mol Med Rep 4 (2), 363-368 (2011)
   PUBMED   21468578
  REMARK    GeneRIF: The present study focused on the expression of
            chondroitin-synthesizing enzymes in colorectal cancer.
REFERENCE   6  (bases 1 to 3857)
  AUTHORS   Izumikawa,T., Uyama,T., Okuura,Y., Sugahara,K. and Kitagawa,H.
  TITLE     Involvement of chondroitin sulfate synthase-3 (chondroitin
            synthase-2) in chondroitin polymerization through its interaction
            with chondroitin synthase-1 or chondroitin-polymerizing factor
  JOURNAL   Biochem J 403 (3), 545-552 (2007)
   PUBMED   17253960
  REMARK    GeneRIF: results suggest that chondroitin polymerization is
            achieved by multiple combinations of chondroitin synthase-1,
            chondroitin synthase-2, and chondroitin-polymerizing factor
REFERENCE   7  (bases 1 to 3857)
  AUTHORS   Yada,T., Sato,T., Kaseyama,H., Gotoh,M., Iwasaki,H., Kikuchi,N.,
            Kwon,Y.D., Togayachi,A., Kudo,T., Watanabe,H., Narimatsu,H. and
            Kimata,K.
  TITLE     Chondroitin sulfate synthase-3. Molecular cloning and
            characterization
  JOURNAL   J Biol Chem 278 (41), 39711-39725 (2003)
   PUBMED   12907687
  REMARK    GeneRIF: The full-length open reading frame consists of 882 amino
            acids and encodes a typical type II membrane protein. This enzyme
            contains a beta 3-glycosyltransferase motif and a beta
            4-glycosyltransferase motif similar to that found in CSS1.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC011360.4, AJ578034.2, AB086062.1 and AJ504664.2.
            
            On Aug 9, 2018 this sequence version replaced NM_175856.4.
            
            Summary: CSS3 is a glycosyltransferase that has both
            glucuronyltransferase and N-acetylgalactosaminyltransferase
            activities (Yada et al., 2003 [PubMed 12907687]).[supplied by OMIM,
            Mar 2008].
            
            ##Evidence-Data-START##
            Transcript exon combination :: AJ578034.2, AB175496.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968189, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000305031.5/ ENSP00000302629.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-7                 AC011360.4         2593-2599           c
            8-74                AJ578034.2         1-67
            75-75               AC011360.4         2525-2525           c
            76-307              AJ578034.2         69-300
            308-308             AC011360.4         2292-2292           c
            309-365             AJ578034.2         302-358
            366-1171            AB086062.1         1-806
            1172-2931           AJ504664.2         344-2103
            2932-3151           AB086062.1         2567-2786
            3152-3857           AJ504664.2         2324-3029
FEATURES             Location/Qualifiers
     source          1..3857
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5q23.3"
     gene            1..3857
                     /gene="CHSY3"
                     /gene_synonym="CHSY2; CSS3"
                     /note="chondroitin sulfate synthase 3"
                     /db_xref="GeneID:337876"
                     /db_xref="HGNC:HGNC:24293"
                     /db_xref="MIM:609963"
     exon            1..1167
                     /gene="CHSY3"
                     /gene_synonym="CHSY2; CSS3"
                     /inference="alignment:Splign:2.1.0"
     CDS             366..3014
                     /gene="CHSY3"
                     /gene_synonym="CHSY2; CSS3"
                     /EC_number="2.4.1.175"
                     /EC_number="2.4.1.226"
                     /note="carbohydrate synthase 2; chondroitin synthase-2;
                     chSy-2; N-acetylgalactosaminyltransferase 3; chondroitin
                     glucuronyltransferase 3;
                     N-acetylgalactosaminyl-proteoglycan
                     3-beta-glucuronosyltransferase 3;
                     glucuronosyl-N-acetylgalactosaminyl-proteoglycan
                     4-beta-N-acetylgalactosaminyltransferase II"
                     /codon_start=1
                     /product="chondroitin sulfate synthase 3"
                     /protein_id="NP_787052.3"
                     /db_xref="CCDS:CCDS34223.1"
                     /db_xref="GeneID:337876"
                     /db_xref="HGNC:HGNC:24293"
                     /db_xref="MIM:609963"
                     /translation="MAVRSRRPWMSVALGLVLGFTAASWLIAPRVAELSERKRRGSSL
                     CSYYGRSAAGPRAGAQQPLPQPQSRPRQEQSPPPARQDLQGPPLPEAAPGITSFRSSP
                     WQQPPPLQQRRRGREPEGATGLPGAPAAEGEPEEEDGGAAGQRRDGRPGSSHNGSGDG
                     GAAAPSARPRDFLYVGVMTAQKYLGSRALAAQRTWARFIPGRVEFFSSQQPPNAGQPP
                     PPLPVIALPGVDDSYPPQKKSFMMIKYMHDHYLDKYEWFMRADDDVYIKGDKLEEFLR
                     SLNSSKPLYLGQTGLGNIEELGKLGLEPGENFCMGGPGMIFSREVLRRMVPHIGECLR
                     EMYTTHEDVEVGRCVRRFGGTQCVWSYEMQQLFHENYEHNRKGYIQDLHNSKIHAAIT
                     LHPNKRPAYQYRLHNYMLSRKISELRYRTIQLHRESALMSKLSNTEVSKEDQQLGVIP
                     SFNHFQPRERNEVIEWEFLTGKLLYSAAENQPPRQSLSSILRTALDDTVLQVMEMINE
                     NAKSRGRLIDFKEIQYGYRRVNPMHGVEYILDLLLLYKRHKGRKLTVPVRRHAYLQQL
                     FSKPFFRETEELDVNSLVESINSETQSFSFISNSLKILSSFQGAKEMGGHNEKKVHIL
                     VPLIGRYDIFLRFMENFENMCLIPKQNVKLVIILFSRDSGQDSSKHIELIKGYQNKYP
                     KAEMTLIPMKGEFSRGLGLEMASAQFDNDTLLLFCDVDLIFREDFLQRCRDNTIQGQQ
                     VYYPIIFSQYDPKVTNGGNPPTDDYFIFSKKTGFWRDYGYGITCIYKSDLLGAGGFDT
                     SIQGWGLEDVDLYNKVILSGLRPFRSQEVGVVHIFHPVHCDPNLDPKQYKMCLGSKAS
                     TFASTMQLAELWLEKHLGVRYNRTLS"
     exon            1168..1451
                     /gene="CHSY3"
                     /gene_synonym="CHSY2; CSS3"
                     /inference="alignment:Splign:2.1.0"
     exon            1452..3857
                     /gene="CHSY3"
                     /gene_synonym="CHSY2; CSS3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3508..3513
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CHSY3"
                     /gene_synonym="CHSY2; CSS3"
                     /note="hexamer: TATAAA"
     polyA_site      3528
                     /gene="CHSY3"
                     /gene_synonym="CHSY2; CSS3"
     regulatory      3722..3727
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CHSY3"
                     /gene_synonym="CHSY2; CSS3"
                     /note="hexamer: ATTAAA"
     polyA_site      3747
                     /gene="CHSY3"
                     /gene_synonym="CHSY2; CSS3"
                     /note="major polyA site"
     regulatory      3815..3820
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CHSY3"
                     /gene_synonym="CHSY2; CSS3"
                     /note="hexamer: AATAAA"
     polyA_site      3857
                     /gene="CHSY3"
                     /gene_synonym="CHSY2; CSS3"
                     /note="major polyA site"
ORIGIN      
        1 actgacaggg tggcagccta ggagcggacg cgttgccgcc gccgctgctc ctcctcctcc
       61 tcctgcagct cctccagctg ccgctgctgc cgccgctgcc gccaccgccg ccgccgggag
      121 aagtttcact cccgaccctt gctcggagcc ccggcccaga gctgagcggg agcccggcag
      181 gcagctgcag cccgcggcag tcgaggcgtc cgcggcgctt cgacctccag ccggtgtcgg
      241 cgcctaggcg gccggctgcg gccgcggctg ggggcgcaaa ggcggaggag gggcgggtgt
      301 gagccgggga aaccgcgtgc cgcgccgcga cagcccagcg agcgtccgcg cccgggacag
      361 ccgcgatggc tgtgcgctct cgccgcccgt ggatgagcgt ggcattaggg ctggtgctgg
      421 gcttcaccgc cgcgtcctgg ctcatcgccc ccagggtggc ggagctgagc gagaggaaga
      481 gacgtggctc cagcctctgc tcctactacg gtcgctctgc tgctggcccc cgcgccggcg
      541 ctcagcagcc gctcccccag ccccagtccc gaccacggca ggagcagtcg ccgccccccg
      601 cgcgccagga tctccagggg ccaccgctgc ccgaggcagc acccgggatc accagttttc
      661 gaagcagccc ctggcagcag ccacctccgc tgcagcagcg gcggcgagga cgcgagcctg
      721 agggcgcgac ggggcttccc ggtgctccag cggccgaggg ggagcccgag gaggaggacg
      781 ggggcgcggc tgggcagcgg agagacggcc ggccggggag tagccacaac ggcagcgggg
      841 acgggggcgc tgccgccccg agcgcccgac cccgggactt cctgtacgtg ggggtgatga
      901 ccgcgcagaa gtacctgggc agccgcgcgc tggccgcgca gcggacctgg gcgcgtttca
      961 tcccgggccg cgtggagttc ttttccagcc agcagccccc caacgccggc cagcccccgc
     1021 cacccctgcc tgtcatcgcg ctaccgggtg tggacgactc ctatcctccc cagaaaaagt
     1081 ccttcatgat gatcaagtac atgcacgacc actacctgga caagtatgag tggttcatgc
     1141 gcgccgacga cgatgtctac atcaaaggtg ataaattaga agagtttctt agatcgctaa
     1201 acagcagtaa gcctctctac ctgggacaga ctggcctggg gaatattgaa gagcttggaa
     1261 agctgggact ggagcctggg gaaaacttct gtatgggagg acctggcatg atctttagcc
     1321 gagaagttct caggaggatg gtgccacata ttggtgaatg ccttagagaa atgtacacga
     1381 ctcatgagga tgtggaagta ggaagatgcg ttcgccgttt tggtgggact cagtgtgtct
     1441 ggtcttacga gatgcaacaa ctgttccatg aaaattatga acacaatcgg aagggttaca
     1501 tccaagacct tcacaatagc aaaatccatg cagccataac acttcatccc aacaaaaggc
     1561 ctgcatacca atacaggctg cataattaca tgctcagccg caaaatttct gaacttcgct
     1621 accgcaccat ccagctccac agggaaagtg ccctgatgag caagctcagt aacacagaag
     1681 tgagcaaaga ggaccagcag ctgggagtga taccttcttt caaccacttc cagcctcggg
     1741 agagaaatga agtgatagaa tgggagttcc tgacagggaa gcttctatac tcagcagctg
     1801 agaaccagcc ccctcgacag agcctcagta gcattttaag aacagcactg gatgataccg
     1861 tcctacaggt gatggagatg atcaatgaga atgccaagag cagaggacgg ctcattgact
     1921 tcaaggaaat tcagtatggc taccgcagag ttaaccccat gcacggggtg gagtacattt
     1981 tggatttact ccttttatac aaaagacaca agggaaggaa actgactgtg ccagtgagac
     2041 gtcatgccta tcttcagcag ttgttcagca agcctttctt cagagagacc gaagagctag
     2101 atgtcaacag tcttgtggag agtattaaca gtgaaactca gtcattctcc tttatatcta
     2161 attctttaaa gatattatct tcttttcaag gtgccaaaga aatgggaggg cacaatgaaa
     2221 agaaagtaca cattctcgtt cctctcatcg gaaggtatga cattttcttg agattcatgg
     2281 agaactttga aaacatgtgt cttatcccaa agcagaatgt aaagttggtc attatccttt
     2341 tcagtaggga ttctggccaa gactccagca agcatattga gctgataaaa gggtaccaga
     2401 acaagtaccc caaagcagaa atgaccctga tcccaatgaa gggagagttt tccagaggtc
     2461 ttggtcttga aatggcttct gcccagtttg acaatgacac tttgctgcta ttttgtgatg
     2521 ttgacttgat cttcagagaa gattttctcc aacgatgtag agacaataca attcagggac
     2581 aacaggtgta ctatcccatc atctttagcc agtatgaccc aaaggtaaca aacgggggaa
     2641 atcctcccac tgatgattac ttcatattct caaaaaagac tggattttgg agagactatg
     2701 gatatggcat cacctgtatt tacaaaagtg atcttctagg tgcaggtgga tttgatacct
     2761 caatacaagg ctggggacta gaagatgtag atctctacaa taaagtcatt ctatctggct
     2821 taaggccatt cagaagccaa gaagtaggag tggtgcatat tttccatcca gttcattgtg
     2881 atcctaactt ggaccctaag cagtataaga tgtgcttagg atccaaggca agtactttcg
     2941 cctcaaccat gcaactggct gaactctggc ttgaaaaaca tttaggtgtc aggtacaatc
     3001 gaactctctc ctgacagtcc aggcaacaca ttttgccttt tttaagggga gtttacctca
     3061 ttgttggttg ttgttatttt tattgtatta ttgttatttt attattatta ttgttataat
     3121 tttattttgt tgtcctggtc ttaaactact cttggttgtc ttcctaaggg tgtttgttga
     3181 cctcaagcaa gaagagtctg cagttcactg atgtttcaga tttctactga agtcaatatg
     3241 ttattacttt tatataactt tatttgagat tgagttaaat catagcaatc aaatgacttt
     3301 tgaaacgcat tcatcacaag agacagctta gaagaatgtt ctcttggggc ttaaagatgc
     3361 aatatcgact tttatttggt ttctcaaatt caatgtgata caaatattta ctggtgaaag
     3421 gtaccacaaa agtgctttat gcttcctatg gggaagggac tctgtaacat aaacttgagt
     3481 tttgtaattt atacaaggac ttaaatatat aaacaataat tttcttcatc tttagaattt
     3541 ttaaagtaaa tgaataccta tgattgtatg tttatttttt aaaaaaagtt ttaaaaattg
     3601 aggagttttg ttccacaagc aaccggtact ggctaccctg gtcttatgac aattatgagc
     3661 tcctcacaac tgtctgctat aaatgcgaat gaactttatt ttctcaagga aatatgattt
     3721 aattaaatat tcatgtacat tttagaagct ttatgaaaca atgtccttca tttgctggca
     3781 agaagataaa atatgacaga acctgtttat ttaaaataaa acacaggtat agcagtattc
     3841 tttttcaaaa acactga
//
