LOCUS       NM_031422              11379 bp    mRNA    linear   PRI 06-JUN-2024
DEFINITION  Homo sapiens carbohydrate sulfotransferase 9 (CHST9), transcript
            variant 1, mRNA.
ACCESSION   NM_031422
VERSION     NM_031422.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 11379)
  AUTHORS   Chen,J., Wu,J.S., Mize,T., Moreno,M., Hamid,M., Servin,F.,
            Bashy,B., Zhao,Z., Jia,P., Tsuang,M.T., Kendler,K.S., Xiong,M. and
            Chen,X.
  TITLE     A Frameshift Variant in the CHST9 Gene Identified by Family-Based
            Whole Genome Sequencing Is Associated with Schizophrenia in Chinese
            Population
  JOURNAL   Sci Rep 9 (1), 12717 (2019)
   PUBMED   31481703
  REMARK    GeneRIF: A Frameshift Variant in the CHST9 Gene Identified by
            Family-Based Whole Genome Sequencing Is Associated with
            Schizophrenia in Chinese Population.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11379)
  AUTHORS   Yuan,J., Zhang,N., Zhu,H., Liu,J., Xing,H., Ma,F. and Yang,M.
  TITLE     CHST9 rs1436904 genetic variant contributes to prognosis of
            triple-negative breast cancer
  JOURNAL   Sci Rep 7 (1), 11802 (2017)
   PUBMED   28924212
  REMARK    GeneRIF: CHST9 rs1436904 polymorphism significantly contributes to
            prognosis of early-stage TNBC, suggesting its clinical potential in
            the screening of high-risk TNBC patients for recurrence and the
            possibility of patient-tailored therapeutic decisions.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 11379)
  AUTHORS   Yang,X., Coulombe-Huntington,J., Kang,S., Sheynkman,G.M., Hao,T.,
            Richardson,A., Sun,S., Yang,F., Shen,Y.A., Murray,R.R., Spirohn,K.,
            Begg,B.E., Duran-Frigola,M., MacWilliams,A., Pevzner,S.J.,
            Zhong,Q., Wanamaker,S.A., Tam,S., Ghamsari,L., Sahni,N., Yi,S.,
            Rodriguez,M.D., Balcha,D., Tan,G., Costanzo,M., Andrews,B.,
            Boone,C., Zhou,X.J., Salehi-Ashtiani,K., Charloteaux,B., Chen,A.A.,
            Calderwood,M.A., Aloy,P., Roth,F.P., Hill,D.E., Iakoucheva,L.M.,
            Xia,Y. and Vidal,M.
  TITLE     Widespread Expansion of Protein Interaction Capabilities by
            Alternative Splicing
  JOURNAL   Cell 164 (4), 805-817 (2016)
   PUBMED   26871637
REFERENCE   4  (bases 1 to 11379)
  AUTHORS   Leger,P.D., Johnson,D.H., Robbins,G.K., Shafer,R.W., Clifford,D.B.,
            Li,J., McLaren,P.J. and Haas,D.W.
  TITLE     Genome-wide association study of peripheral neuropathy with
            D-drug-containing regimens in AIDS Clinical Trials Group protocol
            384
  JOURNAL   J Neurovirol 20 (3), 304-308 (2014)
   PUBMED   24554482
REFERENCE   5  (bases 1 to 11379)
  AUTHORS   Wang,H., St Julien,K.R., Stevenson,D.K., Hoffmann,T.J., Witte,J.S.,
            Lazzeroni,L.C., Krasnow,M.A., Quaintance,C.C., Oehlert,J.W.,
            Jelliffe-Pawlowski,L.L., Gould,J.B., Shaw,G.M. and O'Brodovich,H.M.
  TITLE     A genome-wide association study (GWAS) for bronchopulmonary
            dysplasia
  JOURNAL   Pediatrics 132 (2), 290-297 (2013)
   PUBMED   23897914
REFERENCE   6  (bases 1 to 11379)
  AUTHORS   Michailidou,K., Hall,P., Gonzalez-Neira,A., Ghoussaini,M.,
            Dennis,J., Milne,R.L., Schmidt,M.K., Chang-Claude,J., Bojesen,S.E.,
            Bolla,M.K., Wang,Q., Dicks,E., Lee,A., Turnbull,C., Rahman,N.,
            Fletcher,O., Peto,J., Gibson,L., Dos Santos Silva,I.,
            Nevanlinna,H., Muranen,T.A., Aittomaki,K., Blomqvist,C., Czene,K.,
            Irwanto,A., Liu,J., Waisfisz,Q., Meijers-Heijboer,H., Adank,M., van
            der Luijt,R.B., Hein,R., Dahmen,N., Beckman,L., Meindl,A.,
            Schmutzler,R.K., Muller-Myhsok,B., Lichtner,P., Hopper,J.L.,
            Southey,M.C., Makalic,E., Schmidt,D.F., Uitterlinden,A.G.,
            Hofman,A., Hunter,D.J., Chanock,S.J., Vincent,D., Bacot,F.,
            Tessier,D.C., Canisius,S., Wessels,L.F., Haiman,C.A., Shah,M.,
            Luben,R., Brown,J., Luccarini,C., Schoof,N., Humphreys,K., Li,J.,
            Nordestgaard,B.G., Nielsen,S.F., Flyger,H., Couch,F.J., Wang,X.,
            Vachon,C., Stevens,K.N., Lambrechts,D., Moisse,M., Paridaens,R.,
            Christiaens,M.R., Rudolph,A., Nickels,S., Flesch-Janys,D.,
            Johnson,N., Aitken,Z., Aaltonen,K., Heikkinen,T., Broeks,A.,
            Veer,L.J., van der Schoot,C.E., Guenel,P., Truong,T.,
            Laurent-Puig,P., Menegaux,F., Marme,F., Schneeweiss,A., Sohn,C.,
            Burwinkel,B., Zamora,M.P., Perez,J.I., Pita,G., Alonso,M.R.,
            Cox,A., Brock,I.W., Cross,S.S., Reed,M.W., Sawyer,E.J.,
            Tomlinson,I., Kerin,M.J., Miller,N., Henderson,B.E., Schumacher,F.,
            Le Marchand,L., Andrulis,I.L., Knight,J.A., Glendon,G.,
            Mulligan,A.M., Lindblom,A., Margolin,S., Hooning,M.J.,
            Hollestelle,A., van den Ouweland,A.M., Jager,A., Bui,Q.M.,
            Stone,J., Dite,G.S., Apicella,C., Tsimiklis,H., Giles,G.G.,
            Severi,G., Baglietto,L., Fasching,P.A., Haeberle,L., Ekici,A.B.,
            Beckmann,M.W., Brenner,H., Muller,H., Arndt,V., Stegmaier,C.,
            Swerdlow,A., Ashworth,A., Orr,N., Jones,M., Figueroa,J.,
            Lissowska,J., Brinton,L., Goldberg,M.S., Labreche,F., Dumont,M.,
            Winqvist,R., Pylkas,K., Jukkola-Vuorinen,A., Grip,M., Brauch,H.,
            Hamann,U., Bruning,T., Radice,P., Peterlongo,P., Manoukian,S.,
            Bonanni,B., Devilee,P., Tollenaar,R.A., Seynaeve,C., van
            Asperen,C.J., Jakubowska,A., Lubinski,J., Jaworska,K., Durda,K.,
            Mannermaa,A., Kataja,V., Kosma,V.M., Hartikainen,J.M.,
            Bogdanova,N.V., Antonenkova,N.N., Dork,T., Kristensen,V.N.,
            Anton-Culver,H., Slager,S., Toland,A.E., Edge,S., Fostira,F.,
            Kang,D., Yoo,K.Y., Noh,D.Y., Matsuo,K., Ito,H., Iwata,H., Sueta,A.,
            Wu,A.H., Tseng,C.C., Van Den Berg,D., Stram,D.O., Shu,X.O., Lu,W.,
            Gao,Y.T., Cai,H., Teo,S.H., Yip,C.H., Phuah,S.Y., Cornes,B.K.,
            Hartman,M., Miao,H., Lim,W.Y., Sng,J.H., Muir,K., Lophatananon,A.,
            Stewart-Brown,S., Siriwanarangsan,P., Shen,C.Y., Hsiung,C.N.,
            Wu,P.E., Ding,S.L., Sangrajrang,S., Gaborieau,V., Brennan,P.,
            McKay,J., Blot,W.J., Signorello,L.B., Cai,Q., Zheng,W.,
            Deming-Halverson,S., Shrubsole,M., Long,J., Simard,J.,
            Garcia-Closas,M., Pharoah,P.D., Chenevix-Trench,G., Dunning,A.M.,
            Benitez,J. and Easton,D.F.
  CONSRTM   Breast and Ovarian Cancer Susceptibility Collaboration; Hereditary
            Breast and Ovarian Cancer Research Group Netherlands (HEBON);
            kConFab Investigators; Australian Ovarian Cancer Study Group;
            GENICA (Gene Environment Interaction and Breast Cancer in Germany)
            Network
  TITLE     Large-scale genotyping identifies 41 new loci associated with
            breast cancer risk
  JOURNAL   Nat Genet 45 (4), 353-361 (2013)
   PUBMED   23535729
REFERENCE   7  (bases 1 to 11379)
  AUTHORS   Wang,X., Shaffer,J.R., Zeng,Z., Begum,F., Vieira,A.R., Noel,J.,
            Anjomshoaa,I., Cuenco,K.T., Lee,M.K., Beck,J., Boerwinkle,E.,
            Cornelis,M.C., Hu,F.B., Crosslin,D.R., Laurie,C.C., Nelson,S.C.,
            Doheny,K.F., Pugh,E.W., Polk,D.E., Weyant,R.J., Crout,R.,
            McNeil,D.W., Weeks,D.E., Feingold,E. and Marazita,M.L.
  TITLE     Genome-wide association scan of dental caries in the permanent
            dentition
  JOURNAL   BMC Oral Health 12, 57 (2012)
   PUBMED   23259602
  REMARK    Publication Status: Online-Only
REFERENCE   8  (bases 1 to 11379)
  AUTHORS   Zhao,X., Wu,Q., Fu,X., Yu,B., Shao,Y., Yang,H., Guan,M., Huang,X.,
            Zhang,W. and Wan,J.
  TITLE     Examination of copy number variations of CHST9 in multiple types of
            hematologic malignancies
  JOURNAL   Cancer Genet Cytogenet 203 (2), 176-179 (2010)
   PUBMED   21156230
  REMARK    GeneRIF: the copy number variations of CHST9 have been shown to
            associate with hematologic malignancies
REFERENCE   9  (bases 1 to 11379)
  AUTHORS   Hiraoka,N., Misra,A., Belot,F., Hindsgaul,O. and Fukuda,M.
  TITLE     Molecular cloning and expression of two distinct human
            N-acetylgalactosamine 4-O-sulfotransferases that transfer sulfate
            to GalNAc beta 1-->4GlcNAc beta 1-->R in both N- and O-glycans
  JOURNAL   Glycobiology 11 (6), 495-504 (2001)
   PUBMED   11445554
REFERENCE   10 (bases 1 to 11379)
  AUTHORS   Kang,H.G., Evers,M.R., Xia,G., Baenziger,J.U. and Schachner,M.
  TITLE     Molecular cloning and expression of an
            N-acetylgalactosamine-4-O-sulfotransferase that transfers sulfate
            to terminal and non-terminal beta 1,4-linked N-acetylgalactosamine
  JOURNAL   J Biol Chem 276 (14), 10861-10869 (2001)
   PUBMED   11139592
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AY358488.1, AF239821.1,
            AC010854.3 and KF456359.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_031422.5.
            
            Summary: The protein encoded by this gene belongs to the
            sulfotransferase 2 family. It is localized to the golgi membrane,
            and catalyzes the transfer of sulfate to position 4 of non-reducing
            N-acetylgalactosamine (GalNAc) residues in both N-glycans and
            O-glycans. Sulfate groups on carbohydrates confer highly specific
            functions to glycoproteins, glycolipids, and proteoglycans, and are
            critical for cell-cell interaction, signal transduction, and
            embryonic development. Alternatively spliced transcript variants
            have been described for this gene. [provided by RefSeq, Aug 2011].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660805.193759.1,
                                           SRR1660809.189826.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968832, SAMEA1968968
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000618847.5/ ENSP00000480991.1
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1592              AY358488.1         10-1601
            1593-2229           AF239821.1         1500-2136
            2230-6575           AC010854.3         133612-137957       c
            6576-6918           KF456359.1         1-343
            6919-11379          AC010854.3         128808-133268       c
FEATURES             Location/Qualifiers
     source          1..11379
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="18"
                     /map="18q11.2"
     gene            1..11379
                     /gene="CHST9"
                     /gene_synonym="GALNAC4ST-2; GalNAc4ST2"
                     /note="carbohydrate sulfotransferase 9"
                     /db_xref="GeneID:83539"
                     /db_xref="HGNC:HGNC:19898"
                     /db_xref="MIM:610191"
     exon            1..173
                     /gene="CHST9"
                     /gene_synonym="GALNAC4ST-2; GalNAc4ST2"
                     /inference="alignment:Splign:2.1.0"
     exon            174..390
                     /gene="CHST9"
                     /gene_synonym="GALNAC4ST-2; GalNAc4ST2"
                     /inference="alignment:Splign:2.1.0"
     CDS             270..1601
                     /gene="CHST9"
                     /gene_synonym="GALNAC4ST-2; GalNAc4ST2"
                     /EC_number="2.8.1.-"
                     /note="isoform 1 is encoded by transcript variant 1;
                     GalNAc-4-sulfotransferase 2; N-acetylgalactosamine
                     4-O-sulfotransferase 2; galNAc-4-O-sulfotransferase 2;
                     carbohydrate (N-acetylgalactosamine 4-0) sulfotransferase
                     9"
                     /codon_start=1
                     /product="carbohydrate sulfotransferase 9 isoform 1"
                     /protein_id="NP_113610.2"
                     /db_xref="CCDS:CCDS42422.1"
                     /db_xref="GeneID:83539"
                     /db_xref="HGNC:HGNC:19898"
                     /db_xref="MIM:610191"
                     /translation="MQPSEMVMNPKQVFLSVLIFGVAGLLLFMYLQVWIEEQHTGRVE
                     KRREQKVTSGWGPVKYLRPVPRIMSTEKIQEHITNQNPKFHMPEDVREKKENLLLNSE
                     RSTRLLTKTSHSQGGDQALSKSTGSPTEKLIEKRQGAKTVFNKFSNMNWPVDIHPLNK
                     SLVKDNKWKKTEETQEKRRSFLQEFCKKYGGVSHHQSHLFHTVSRIYVEDKHKILYCE
                     VPKAGCSNWKRILMVLNGLASSAYNISHNAVHYGKHLKKLDSFDLKGIYTRLNTYTKA
                     VFVRDPMERLVSAFRDKFEHPNSYYHPVFGKAIIKKYRPNACEEALINGSGVKFKEFI
                     HYLLDSHRPVGMDIHWEKVSKLCYPCLINYDFVGKFETLEEDANYFLQMIGAPKELKF
                     PNFKDRHSSDERTNAQVVRQYLKDLTRTERQLIYDFYYLDYLMFNYTTPFL"
     exon            391..429
                     /gene="CHST9"
                     /gene_synonym="GALNAC4ST-2; GalNAc4ST2"
                     /inference="alignment:Splign:2.1.0"
     exon            430..471
                     /gene="CHST9"
                     /gene_synonym="GALNAC4ST-2; GalNAc4ST2"
                     /inference="alignment:Splign:2.1.0"
     exon            472..509
                     /gene="CHST9"
                     /gene_synonym="GALNAC4ST-2; GalNAc4ST2"
                     /inference="alignment:Splign:2.1.0"
     exon            510..11379
                     /gene="CHST9"
                     /gene_synonym="GALNAC4ST-2; GalNAc4ST2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2064..2069
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CHST9"
                     /gene_synonym="GALNAC4ST-2; GalNAc4ST2"
                     /note="hexamer: GATAAA"
     polyA_site      2084
                     /gene="CHST9"
                     /gene_synonym="GALNAC4ST-2; GalNAc4ST2"
                     /note="major polyA site"
     regulatory      2145..2150
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CHST9"
                     /gene_synonym="GALNAC4ST-2; GalNAc4ST2"
                     /note="hexamer: AATATA"
     polyA_site      2162
                     /gene="CHST9"
                     /gene_synonym="GALNAC4ST-2; GalNAc4ST2"
                     /note="major polyA site"
     regulatory      11357..11362
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CHST9"
                     /gene_synonym="GALNAC4ST-2; GalNAc4ST2"
                     /note="hexamer: AATAAA"
     polyA_site      11379
                     /gene="CHST9"
                     /gene_synonym="GALNAC4ST-2; GalNAc4ST2"
ORIGIN      
        1 agggtgagcg cccgactccg agctgtcccc gctcccggcg cggcgctccg ctctcagcca
       61 cctcacggct gccaggagtg cgcgggagtt tgccccggag cgcggggaag tttcctccga
      121 agctgcgctc ctggaacagc agcacctgca agcgcccggc agcggcccgc gaggttactt
      181 tatggaattg ggctcttaga gaacaagaaa agactgaagt tttacgggaa aacaaatcat
      241 gtggtcttca gattctgaaa taaggagaaa tgcagccatc tgaaatggtc atgaacccca
      301 aacaagtctt cctctctgtg ctgatatttg gagtagctgg gctactcctc ttcatgtatt
      361 tgcaagtctg gattgaagaa caacatacag ggagagtgga gaagagaaga gaacaaaaag
      421 taacttcagg atggggacca gtgaagtact tgcggcctgt acccagaatc atgagtacag
      481 aaaaaatcca ggaacatatc accaaccaga accccaagtt tcacatgcct gaggatgtac
      541 gagaaaaaaa ggaaaatctt ctactcaatt ctgagagatc tactaggctc ttaacaaaga
      601 ccagtcattc acaaggaggg gatcaagctt taagtaagtc cacagggtca ccaacagaga
      661 agttgattga aaaacgtcaa ggagctaaga ctgtttttaa caagttcagc aacatgaatt
      721 ggccagtgga cattcaccct ttaaacaaaa gtttagtcaa agataataaa tggaagaaaa
      781 ctgaggagac ccaagagaaa cgaaggtctt tccttcagga gttttgcaag aaatacggtg
      841 gggtgagtca tcatcagtca catctttttc atacagtatc cagaatctat gtagaagata
      901 aacacaaaat cttatattgt gaggtaccta aggctggctg ttccaattgg aaaagaattc
      961 tgatggtact aaatggattg gcttcctctg catacaacat ctcccacaat gctgtccact
     1021 acgggaagca tttgaagaag ctagatagct ttgacctaaa agggatatat acccgcttaa
     1081 atacttacac caaagctgtg tttgttcgtg atcccatgga aagattagta tcagccttta
     1141 gggacaaatt tgaacacccc aatagttatt accatccagt attcggaaag gcaattatca
     1201 agaaatatcg accaaatgcc tgtgaagaag cattaattaa tggatctgga gtcaagttca
     1261 aagagtttat ccactacttg ctggattccc accgtccagt aggaatggac attcactggg
     1321 aaaaggtcag caaactctgc tatccgtgtt tgatcaacta tgattttgta gggaaatttg
     1381 agactttgga agaagatgcc aattactttt tacagatgat cggtgctcca aaggagctga
     1441 aatttcccaa ctttaaggat aggcactctt ccgatgaaag aaccaatgct caagtcgtga
     1501 gacagtattt aaaggatctg actagaactg agagacaatt aatctatgac ttttattact
     1561 tggactattt aatgtttaat tatacaactc catttttgta gtttgcattc attttctaaa
     1621 accctgtata tacttaatga tgataagttc aaatcagctg taatttttct ataattctct
     1681 gtatgacaga aatttaacca agtgcagttg tcttgattta atgtagattt ttaccaaata
     1741 gtatgacacc aattggcaca aagttatagg aaaatcacct acaggagatg taaacaactt
     1801 gagttgctct aaaatgtttg gaaaagagct gcttttgcat tatgaattat attgttgaag
     1861 caataaccta gccagctgtt gcattagcta aagcagcctc ttgcaatggt aggaaaaaag
     1921 gatctcaaat agcatgagtg tatgtctata tcctgaaatt tattgtctaa aatgcatgaa
     1981 tatattttta gcagtctgtg gcatattaat caaactgttg aattgttttc ttacaccctg
     2041 gaaatctttc tatcaactat aatgataaat ccattttgaa gtgatatttt ggacttaggc
     2101 attttacttt agattggaag gcattatgtg atttacaata tgagaatata gcagaaaaac
     2161 cagatgaggc tgtggctttt tatattcaac agccaataaa aaatgcacaa catgctaaga
     2221 tcaaagcaac aaaaccaacc ttcttcttcc agaaaaactt aagggaattg attctgtttt
     2281 cttttgagcc ttccacaaag agacaaaatg aacacaagca ctaaaggtct acatttctga
     2341 agcaggcagt tgagaagctt aagtctcatc agatataaat gtgctcctaa ttctggttta
     2401 attgagcagg ggggaaattg tttcaggatg gaataatcat caaaaacaaa agttgccatt
     2461 ctgaatatgg aactcaaaca ataacaaagt tttataagta tattatttga atcaaagcat
     2521 catgtgcata gctttatatg tggaatacac cctaagaata gcttttgttt tcagtttaat
     2581 tattcagctt aggaatgact ttttaaaaac taatatacca cttcaaagat aggttatgtt
     2641 ctagagaata aacataaaaa gagcccctag aaatgtgttt aataggctta tgcataagta
     2701 aattaaggta ttttcttttt aagtttaaat attctatact aacaaagtca gataccattg
     2761 cattcaaata ttaaatcaaa gtccttagaa aaataggtcc atgtgtcacc cttatgaata
     2821 gttgtaatgc attttggaat ttttttccaa gggatcctaa attagatccc ttggggaaaa
     2881 aaaaagggat cattttggat gggaaacatt tggatgggaa acctacttta attggtcgac
     2941 ttttaccccg agttaagaaa atcacaagtc tttcaaaccc tgcttaaatg cttttcctga
     3001 acatcttcct cttcctaagc tatcattttt ttcttatcag taaatattca gtaatggctt
     3061 tcaaagaaca gtaggtatta atagttgaat gttaatagac caagatagtt gcagtcatac
     3121 ttatttcatt ttgattttct atttcatggc tgagtattca tgaattgctt cgctccttac
     3181 acacacctgc cgtgtcccat ctgtgtctcc taaggagtaa gtaaactgca atgggctgag
     3241 agatcaaaag tattaaacct tctaatccaa ccttttacta cttttccatg ccattaactc
     3301 ttgcctctag accatacact taagctgtgc tctcagtttt taagacgttt tactctggag
     3361 taatctaaat tacataattt aaaattttgc tgcaccatgt agagtagatt cctagtaatt
     3421 tagggaaatt gaaattatct ttttatggag agagaaaata aaaaatattc cagaagcctg
     3481 aaattttatt cctaagaaac acaaaacttg ctttatatga aatccactat aaagaataaa
     3541 ggcaatttcc tcaaaagtat tcctaaaact atataaggaa cagacaaaga actttcaata
     3601 gctttagaag gaaaaaaatg gaaacaacag tcctacctct cttattggca tttgtaatgc
     3661 attgggcagg gtggaatgca tacctatata aatgtttggc tttgatggtg gagaggaggg
     3721 atgagtggaa gaaaatgata cccagtgttc tctacggaaa gctgtattgt accttttgct
     3781 gtatatgttc ctttcctgac cctttccaac gagcccgtgt caccatgggt cattcttttc
     3841 ctaaatgggc tctatgatgc caagtgacac cctgtgtagt tgcaagttcc aaaagccatg
     3901 catgtctttg ccaagtaaga acaaactttt tatgtacctg ctcttcattt tagatggctt
     3961 attttcatgt taggaccaaa gaagccagtc atcatcctgg attacttgtg agtttaaagc
     4021 caaactccat ttttctctcc tgacttattt ggccttgcag accagctcac attcaccttt
     4081 gtcatttctg cctgtgcata aatccaggtg ggaacaccac tgttctttag gaccatcaca
     4141 atttcatgaa tttaattcct aaaggcaagt gaccaaatcc agactcatct tccagatgtc
     4201 caaagttccc attttaacat taggtgcaat tataagtcct ccaattaaac cctaaggatc
     4261 ctatttgttt ttataggcag cagtaacaca atgggcagac cccttcgctc tgttattccc
     4321 ctgtctggaa ttactatcag ggtaacaagt tggcaattaa atccaaaacc acagttatgt
     4381 aacctgtaaa ctatctatag atgtgcaata ttgatgtgat tgtgcccaga aactgccata
     4441 aacttcaatg gaaatgtatg tacaggacat ccagttttcg gattttctca aacatttgga
     4501 aatatccatt aattcaatgg aaatgtgatg ccacttgcta gatgtctata aaacattctg
     4561 taacagaaaa taataatatt attatcatta gggaattagt agttctaatt caagaactac
     4621 ttgtatgtca tttttaatta gaaagaggaa aaatactttt acattttatt gatgtattat
     4681 tattcttatc tcctaaaatg tatagtattt agcagaaggt atatataaga agatgcagag
     4741 aattttgaat attagagtgc cctttgaaaa aatcacttgc tgaattaata gaacttggga
     4801 ttaggctagg actacagtaa acgtctccct cctgtgcatt ctcatgtggc ctctcagggc
     4861 atcccagcct ctaggccttc atggggcgtg gtgaagcaga tgttttgccc agggctaacc
     4921 ctcccaagat gctctgatac ttgaaaaagg attggccaaa ggcgtttaat tatagctcaa
     4981 ctacctgaga accttaggcc aaggctaatt caatttagtc ctacatttag gctcatacct
     5041 tctcaatctc aatcatgatc attatacagt aatcgtatgc atgatcctag ctaacattta
     5101 ttcaatactt actgggtgtc aggcactctt caagtagttt acatgcatta atttaatctc
     5161 catagccagt ctaacatagg taatattatg tagccctctc cctttccatc tatcataaag
     5221 gaaagtaaag cagactatag ctgagtaact tgcctaagga gacagagatg ataagtaggt
     5281 cagaagccag gatgaagcca agcagcaaat accactacca aactctttgc ttagaatatt
     5341 acaaaccaat atttactttt gctttccgtt acctctattc aggataggta gaggcaaaaa
     5401 caatatattg gaatcctgaa attttttcca tacaaatatg ggtgtctgtg tgtgtgtgtg
     5461 tgtgtgtgtg tgtgtgtgtg tgtgtattta gctagttagt tatcacattt caaatcagct
     5521 tatttcttta ttcctgctga gttaaactga caggttcaaa gactttggaa gaaattaggt
     5581 ttcctcccca caactttttt gtaatcctta gttaatcatg gaaacaatgt ttttaagaag
     5641 aaaaaatagt ccctggaaca aatttagcct tgtgaaaaca aaatatcatt tctttgacaa
     5701 gaaattccag acagctacaa gaccagtcaa ggataagcag aatctaagag gaagaaagtt
     5761 caggcgaact ttccacacat aggcccctga cagccactgc agatggtcac ggttcagcaa
     5821 gctagtcaca cacagtgaga accactcctg ctagaaacag agcaactgtg cattatcccc
     5881 ttcttcctga gtctccttcc ctagtactag tttgtagtca gattaccctg agcctacacg
     5941 ggaagactgc agaaaagaat agaggagcaa gctgacttaa accgactgaa aaaacaccat
     6001 gaaaccaggt gaaaataaat aagctcttgc tagaagatgg cagacatttc ccaggcctgt
     6061 gatcattacc tgaggaaggc caggtctggg gagttcaaca gaacattgcc tcatatcatt
     6121 tcattctcag aatttcctag tcagtcacat cttagttttt caccaggatc atatgcttcc
     6181 aggcaaaagt atttaaatcc atttaagcag tcatcttttt ccatccctaa acctctcctc
     6241 aagtctcaca ctatatttgg aatgctcccc tctctatata tctggacatt catttcttgt
     6301 taactcacca aggaatctct tgagattatt caatttcaaa atctcatctc tttatgagct
     6361 ccctgatagg cagaaaaaga cagcagagtt ttcccctggt gtaacatgca tcagattatt
     6421 ctcttaccaa taaatgcagg cattcctttc ctcgattttt tttgtcaggg aggttcgtta
     6481 atttatttgt tcatgtgcta tttatctcac tttttatgaa tataaaatat ctgttgttgt
     6541 cattggaatg tcttaggtct tccaagagga aaggaactgt gtctgttgaa tgcactttgt
     6601 accatgtcta gcaaggcact cagtggaaag cgttacttta acagatgctt ttgatgctgt
     6661 tgtttaaagg ttcctgtaca acttctttta gtgtagaatt cgtatatttc tgcctgtgac
     6721 cttcaggcat ctataggacc agaggatctg aaaggaaggt gctagagcag ggcagctgag
     6781 ggaagggagc tgagccacac accttttcta cactaatagt catcccttga cctgaaggat
     6841 agttgcagac acagaaatct tttacatttc taagaaatag cagttgcttg attgtttggg
     6901 gaagaaaatt ttgagaaaaa tgcatgattc tggtcaacat aagagccatt tactgaggat
     6961 ctactcagta gcagacacgg gaaaaatact ttgcatacat tcatttaatg acatgaagag
     7021 taagtctaac cgtttccaaa agtagctttc aaaaaaatca aagtcgagta tttttccacc
     7081 tatcatttca tgaatgagaa aactgagtat caaagaggtt taataacagt ctctctggct
     7141 ccaaagctga gaccatacat tggcagccca cggggtagat ctggctacag atatgtttgg
     7201 tttggctgac agtgcatttt atttaacatc tgaattagct gccaacattg ggcctcatta
     7261 atgacagggg atttgttacc ctacattcta tatgcccacc tgaaaatagt tggacagagg
     7321 caaggtcagt ccctttagat gaggttggag atctctagtt gttcacactc ttcactaatc
     7381 caccatcact cattcacctt acttgcctgg cccctaagca tctcactttt caagtcctgc
     7441 tctgaagagt gtattccttc ccccgccaag cttccaaagg aaatcaactc ctgaacatta
     7501 tccttgaccc tatttgtgtt gtgcaggtag tgaggttatc ccattccggg tttccatatc
     7561 cttgctcaca gtgctgcttg ccctttggtc ctatatccag tgggatctcc aaacagtatt
     7621 tcattgtttg gggaagtctc ttttgaccaa tgacaaagat gttgggagaa atccagtacc
     7681 tttttttggc atctttctgt cgtagccact gggtcccctg gcaacgacat tccttcccac
     7741 ctctttctgc agagatgagt cattctccca ggctcttgga gctctagtat ctagggacag
     7801 aaactggctc ccactgctgc ctctagactg ctgttctata accttcatcc aaaaatctct
     7861 tctgttttgc aggtgtgccc gcttcctcaa ctctaaaaat cacctgcttc ccagccttct
     7921 ccgaagattc ttctttctga tttataacat gcctctccac cctacttcct gctattatct
     7981 gggtgattgt agcaccccct gtaacattct actctcttgc ttcttaatgc tctgacaacc
     8041 tcctcctcct cctcttccct cctcttcctc ctcctcctcc tactcctcct actcctcccc
     8101 ccacttctat acttagagag gtggagaaaa caggagaagg gataactacg acttttgtcc
     8161 tcaaatacca ggaaaactag taaaacacac agtatctcat tttatgcatt catttgctga
     8221 aaatatttcc aaagtttaga aaaatgattt ttggatgtac tatataataa ataaaaatgc
     8281 actcagaatg ctagagtgtc taccaactta aaagaacaat attatttgtt tgactgtgat
     8341 tatccttagg aaaaaataag ccctgattta acttatcaat ttctgaaaaa aatagtgtat
     8401 ggacgacctg tatcagaatt aacctaattt ttgttaaaaa catattccca gaccccatcc
     8461 aagatacaca gaattggaat ctctatgaaa accacctgaa aatttccttt ttaataatct
     8521 cttggggtac ttctcgtgtg tgtgtgaatt ttttaagtcc tggttgatta taaatgataa
     8581 tacaacctgc tcttttcatt ctgtcactgt gttgaaaagt taacaactct cagctcagag
     8641 aattgtcagt gggtattggt accaaagtcc tctgtgggat tttgaccagc actgaagcag
     8701 aaacttggcg tccgaagtct gccaagtgca agaactagag ctgaaatgat gaagtgaggg
     8761 acggggacag cactgggcac tgcccaaact ttttgaaagc tttcagaatg aaatggggaa
     8821 atggaaactt tcctctgttt gaaatataaa agacaaatgt gtactgaagg caattttggc
     8881 aactcaactt cacattgaac aagtgaagac aatatgagtt tctccaataa aaactaaaaa
     8941 gctgttttca aattgaccta agatgattga aaatggaaaa tatttgctat ttggttatta
     9001 gtttgagagc aagtgcccca ggaaatattt tgcaatttaa cttaaatggt tataatgtat
     9061 attttcttga acttcattaa aggatttgtc tctttttgcc tttctattag gttgaaccat
     9121 atgacattgc caacatttga ctgactctga catataaaca cagccctttc atgtgggtcc
     9181 actaacagtt ctgctgctac ctacccaatt ctaaccacat tggctctcct cagaactatt
     9241 ggaaatagcc tctgaataga agtcctgcct tttttattta tttatttaaa aattacttct
     9301 tttctctttg catttttctt cttttgatat tttcaattgt ggcaaaatcc acataacata
     9361 aaattttatc accttatcat tttttggtat ccttttaatg aatattttcc tatccctccc
     9421 catcttacct attttttttt ttgagacaga gtttcactct tgtcgcccag gctgcagtgc
     9481 agtggcacca tctctgctca ctgcaacctc cacgtcctgg gttcaagcta ttctcctgtc
     9541 tcagcctccc gagtagctgg gactacaggt gcacagcacc acgcccagct aatttttgta
     9601 tttttagtag agattgggtt tcaccacatt ggccaggatg gtctcgatct cttgacctcg
     9661 tgacccatcc gccacagcct cccaaagtgc tgggattaca ggcatgagcc accatgcctt
     9721 gccaccattt tttaagtgta cagttcagca gcatttagtg tagttgtgca agcatcacca
     9781 ctatcccatc acataccttc cttccttgtg aaaaatggat actctgcacc tattaaacaa
     9841 cttcactttt tgccctcccc cagttctggc aaccatcatt ctaccttttg tctctatgaa
     9901 tttgataact taaaatacct catataaatg gaatcataca gcatgtgtct ttttgtgact
     9961 ggcgtatttc tcttagcata atgtcctcaa ggttcatcca tattgaagga cgtaccagaa
    10021 tttttttctt ttcaaggctg aattgtattc cattctcatt gcctggcctc aatctctcaa
    10081 gactataacc cattcccagc ttaatcacct gatagctgca tcaaacccac tattatccag
    10141 ccacatggaa ccattacatt ctctgaaaac acctcttgct ttttccccta ttttgctcac
    10201 actattacac gacctgaagt gtcctttatt cctctttaag gacctccaca tgggtgcccc
    10261 ctccaagagt tgcctgcccc gcatgaccat cccacattca gctcgctcta ccctctctgc
    10321 ttggggactc tcttccactc tccttgcttt agctccacca ttaggctgat ggttccgaaa
    10381 tctttttcta ggctatgttt ctatcacgaa ctttgattca gattcccagt ttcttgtggg
    10441 actgcttcat ctagatgtct cactggagct tccaactcaa catgaccaaa gctgaactca
    10501 tcacctaacc cccaaacctg ctttctcctt atttggtggc aacaccatcc atctactcca
    10561 tcttctgagt taggaaccta gaagtcaacc ccgatttctc cctaagctga ctcctcacag
    10621 ccacagaatt cagaaagccc attaacttca ctgcctgact ttttctcaag cttctcctct
    10681 cctcccatcc attctgctaa tatctaagtt cagactctca ccttctcctg tctacaactc
    10741 tacgataatc ttgaacttct ctcccactct catttccctc aatgccatcc tccatacagc
    10801 tgcccttgta atcactttaa aatgcaaatc caattccagt tctgtttccc actatacatt
    10861 ctatatcctg catgatccca attcttctcc aggcatattt ctggccattt tctgcctcct
    10921 ggcttaatct ccaacagcct agaatggaca taatacccta acattggata gttttcttcc
    10981 tcctgggtct actcagcttg tccctctatt agggcaccca cctacccccc ttcacctccc
    11041 caacacttcc taatccctaa actaagctca gggaggcatt tgtcatctct gcctggctca
    11101 gatgagatgc tctgccctga gtactatgag agattgggaa gcaggattct atgtaggatg
    11161 gttaggagcc tggggcttgt catgagtaag aactgggttt gaattccgta tcctcaactt
    11221 tcaaatgttg ggacctctga aattgcttta acttctctca gtgccagttt tctcatctga
    11281 aaactgagga tagaatacct acctagtaag gggggtatgg gaaatgcatg taaagtattt
    11341 aataagtgct caataaaata aaaccattga tttatctca
//
