LOCUS       NM_001166395            2159 bp    mRNA    linear   PRI 02-APR-2024
DEFINITION  Homo sapiens carbohydrate sulfotransferase 4 (CHST4), transcript
            variant 2, mRNA.
ACCESSION   NM_001166395
VERSION     NM_001166395.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Yu,S.Y., Hsiao,C.T., Izawa,M., Yusa,A., Ishida,H., Nakamura,S.,
            Yagi,H., Kannagi,R. and Khoo,K.H.
  TITLE     Distinct substrate specificities of human
            GlcNAc-6-sulfotransferases revealed by mass spectrometry-based
            sulfoglycomic analysis
  JOURNAL   J Biol Chem 293 (39), 15163-15177 (2018)
   PUBMED   30093410
  REMARK    GeneRIF: mRNA transcript analyses on the genes involved in
            synthesizing GlcNAc-6-O-sulfated glycans in human colon cancer
            tissues indicated that GlcNAc6ST-2 (CHST4) is preferentially
            expressed in cancer cells ...  GlcNAc6ST-3 (CHST5) was only
            expressed in nonmalignant epithelial cells, whereas GlcNAc6ST-1
            (CHST2) was expressed equally in both cancerous and nonmalignant
            epithelial cells.
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Hoshino,H., Ohta,M., Ito,M., Uchimura,K., Sakai,Y., Uehara,T.,
            Low,S., Fukushima,M. and Kobayashi,M.
  TITLE     Apical membrane expression of distinct sulfated glycans represents
            a novel marker of cholangiolocellular carcinoma
  JOURNAL   Lab Invest 96 (12), 1246-1255 (2016)
   PUBMED   27748735
  REMARK    GeneRIF: Data suggest that GlcNAc6ST-2 (CHST4) is critical for
            biosynthesis of MECA-79 sulfated glycans in apical membranes of
            small-sized intrahepatic bile ducts and of cholangiolocellular
            carcinoma (CoCC) cells; MUC1 protein decorated with
            GlcNAc6ST-2-dependent MECA-79 sulfated glycans may serve as a
            useful CoCC marker.
REFERENCE   3  (bases 1 to 2159)
  AUTHORS   Wu,Z.L., Robey,M.T., Tatge,T., Lin,C., Leymarie,N., Zou,Y. and
            Zaia,J.
  TITLE     Detecting O-GlcNAc using in vitro sulfation
  JOURNAL   Glycobiology 24 (8), 740-747 (2014)
   PUBMED   24799377
  REMARK    GeneRIF: A method for O-GlcNAc detection using in vitro sulfation
            with two N-acetylglucosamine (GlcNAc)-specific sulfotransferases,
            carbohydrate sulfotransferase 2 and carbohydrate sulfotransferase
            4.
REFERENCE   4  (bases 1 to 2159)
  AUTHORS   Seko,A., Kataoka,F., Aoki,D., Sakamoto,M., Nakamura,T., Hatae,M.,
            Yonezawa,S. and Yamashita,K.
  TITLE     N-Acetylglucosamine 6-O-sulfotransferase-2 as a tumor marker for
            uterine cervical and corpus cancer
  JOURNAL   Glycoconj J 26 (8), 1065-1073 (2009)
   PUBMED   19156517
  REMARK    GeneRIF: GlcNAc6ST2 could therefore be a good serological marker
            for detecting early-stage uterine cervical and corpus cancers.
REFERENCE   5  (bases 1 to 2159)
  AUTHORS   Uchimura,K. and Rosen,S.D.
  TITLE     Sulfated L-selectin ligands as a therapeutic target in chronic
            inflammation
  JOURNAL   Trends Immunol 27 (12), 559-565 (2006)
   PUBMED   17049924
  REMARK    Review article
REFERENCE   6  (bases 1 to 2159)
  AUTHORS   Uchimura,K., El-Fasakhany,F.M., Hori,M., Hemmerich,S., Blink,S.E.,
            Kansas,G.S., Kanamori,A., Kumamoto,K., Kannagi,R. and Muramatsu,T.
  TITLE     Specificities of N-acetylglucosamine-6-O-sulfotransferases in
            relation to L-selectin ligand synthesis and tumor-associated enzyme
            expression
  JOURNAL   J Biol Chem 277 (6), 3979-3984 (2002)
   PUBMED   11726653
REFERENCE   7  (bases 1 to 2159)
  AUTHORS   Yeh,J.C., Hiraoka,N., Petryniak,B., Nakayama,J., Ellies,L.G.,
            Rabuka,D., Hindsgaul,O., Marth,J.D., Lowe,J.B. and Fukuda,M.
  TITLE     Novel sulfated lymphocyte homing receptors and their control by a
            Core1 extension beta 1,3-N-acetylglucosaminyltransferase
  JOURNAL   Cell 105 (7), 957-969 (2001)
   PUBMED   11439191
REFERENCE   8  (bases 1 to 2159)
  AUTHORS   Li,X., Tu,L., Murphy,P.G., Kadono,T., Steeber,D.A. and Tedder,T.F.
  TITLE     CHST1 and CHST2 sulfotransferase expression by vascular endothelial
            cells regulates shear-resistant leukocyte rolling via L-selectin
  JOURNAL   J Leukoc Biol 69 (4), 565-574 (2001)
   PUBMED   11310842
REFERENCE   9  (bases 1 to 2159)
  AUTHORS   Hemmerich,S., Lee,J.K., Bhakta,S., Bistrup,A., Ruddle,N.R. and
            Rosen,S.D.
  TITLE     Chromosomal localization and genomic organization for the
            galactose/ N-acetylgalactosamine/N-acetylglucosamine
            6-O-sulfotransferase gene family
  JOURNAL   Glycobiology 11 (1), 75-87 (2001)
   PUBMED   11181564
REFERENCE   10 (bases 1 to 2159)
  AUTHORS   Bistrup,A., Bhakta,S., Lee,J.K., Belov,Y.Y., Gunn,M.D., Zuo,F.R.,
            Huang,C.C., Kannagi,R., Rosen,S.D. and Hemmerich,S.
  TITLE     Sulfotransferases of two specificities function in the
            reconstitution of high endothelial cell ligands for L-selectin
  JOURNAL   J Cell Biol 145 (4), 899-910 (1999)
   PUBMED   10330415
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA045171.1, BC035282.1 and
            AC010547.9.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jun 2, 2019 this sequence version replaced NM_001166395.1.
            
            Summary: This gene encodes an N-acetylglucosamine 6-O
            sulfotransferase. The encoded enzyme transfers sulfate from
            3'phosphoadenosine 5'phospho-sulfate to the 6-hydroxyl group of
            N-acetylglucosamine on glycoproteins. This protein is localized to
            the Golgi and is involved in the modification of glycan structures
            on ligands of the lymphocyte homing receptor L-selectin. Alternate
            splicing in the 5' UTR results in multiple transcript variants that
            encode the same protein. [provided by RefSeq, Oct 2009].
            
            Transcript Variant: This variant (2) has an alternate 5' UTR exon,
            as compared to variant 1. Variants 1 and 2 encode the same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC035282.1, AF280088.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000539698.4/ ENSP00000441204.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-11                DA045171.1         176-186
            12-2001             BC035282.1         1-1990
            2002-2159           AC010547.9         144518-144675
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="16"
                     /map="16q22.2"
     gene            1..2159
                     /gene="CHST4"
                     /gene_synonym="GlcNAc6ST2; GST3; HECGLCNAC6ST; LSST"
                     /note="carbohydrate sulfotransferase 4"
                     /db_xref="GeneID:10164"
                     /db_xref="HGNC:HGNC:1972"
     exon            1..70
                     /gene="CHST4"
                     /gene_synonym="GlcNAc6ST2; GST3; HECGLCNAC6ST; LSST"
                     /inference="alignment:Splign:2.1.0"
     exon            71..2159
                     /gene="CHST4"
                     /gene_synonym="GlcNAc6ST2; GST3; HECGLCNAC6ST; LSST"
                     /inference="alignment:Splign:2.1.0"
     CDS             89..1249
                     /gene="CHST4"
                     /gene_synonym="GlcNAc6ST2; GST3; HECGLCNAC6ST; LSST"
                     /EC_number="2.8.2.-"
                     /note="N-acetylglucosamine 6-O-sulfotransferase 2;
                     carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 4;
                     high endothelial cells N-acetylglucosamine
                     6-O-sulfotransferase; L-selectin ligand sulfotransferase;
                     galactose/N-acetylglucosamine/N-acetylgalactosamine
                     6-O-sulfotransferase 3; GST-3; gn6st-2; glcNAc6ST-2;
                     HEC-GlcNAc6ST;
                     galactose/N-acetylglucosamine/N-acetylglucosamine
                     6-O-sulfotransferase 3"
                     /codon_start=1
                     /product="carbohydrate sulfotransferase 4"
                     /protein_id="NP_001159867.1"
                     /db_xref="CCDS:CCDS10902.1"
                     /db_xref="GeneID:10164"
                     /db_xref="HGNC:HGNC:1972"
                     /translation="MLLPKKMKLLLFLVSQMAILALFFHMYSHNISSLSMKAQPERMH
                     VLVLSSWRSGSSFVGQLFGQHPDVFYLMEPAWHVWMTFKQSTAWMLHMAVRDLIRAVF
                     LCDMSVFDAYMEPGPRRQSSLFQWENSRALCSAPACDIIPQDEIIPRAHCRLLCSQQP
                     FEVVEKACRSYSHVVLKEVRFFNLQSLYPLLKDPSLNLHIVHLVRDPRAVFRSRERTK
                     GDLMIDSRIVMGQHEQKLKKEDQPYYVMQVICQSQLEIYKTIQSLPKALQERYLLVRY
                     EDLARAPVAQTSRMYEFVGLEFLPHLQTWVHNITRGKGMGDHAFHTNARDALNVSQAW
                     RWSLPYEKVSRLQKACGDAMNLLGYRHVRSEQEQRNLLLDLLSTWTVPEQIH"
     regulatory      1906..1911
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CHST4"
                     /gene_synonym="GlcNAc6ST2; GST3; HECGLCNAC6ST; LSST"
                     /note="hexamer: ACTAAA"
     regulatory      1919..1924
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CHST4"
                     /gene_synonym="GlcNAc6ST2; GST3; HECGLCNAC6ST; LSST"
                     /note="hexamer: AATAAG"
     polyA_site      1934
                     /gene="CHST4"
                     /gene_synonym="GlcNAc6ST2; GST3; HECGLCNAC6ST; LSST"
     polyA_site      1941
                     /gene="CHST4"
                     /gene_synonym="GlcNAc6ST2; GST3; HECGLCNAC6ST; LSST"
                     /note="major polyA site"
     regulatory      1968..1973
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CHST4"
                     /gene_synonym="GlcNAc6ST2; GST3; HECGLCNAC6ST; LSST"
                     /note="hexamer: AGTAAA"
     polyA_site      2001
                     /gene="CHST4"
                     /gene_synonym="GlcNAc6ST2; GST3; HECGLCNAC6ST; LSST"
                     /note="major polyA site"
ORIGIN      
        1 agaggagaaa agcgcatggc ccggctagca gtgagcctct caaaagcagc agggaagccc
       61 aagccacaag gtcttccact tcagcacaat gctactgcct aaaaaaatga agctcctgct
      121 gtttctggtt tcccagatgg ccatcttggc tctattcttc cacatgtaca gccacaacat
      181 cagctccctg tctatgaagg cacagcccga gcgcatgcac gtgctggttc tgtcttcctg
      241 gcgctctggc tcttcttttg tggggcagct ttttgggcag cacccagatg ttttctacct
      301 gatggagccc gcctggcacg tgtggatgac cttcaagcag agcaccgcct ggatgctgca
      361 catggctgtg cgggatctga tacgggccgt cttcttgtgc gacatgagcg tctttgatgc
      421 ctacatggaa cctggtcccc ggagacagtc cagcctcttt cagtgggaga acagccgggc
      481 cctgtgttct gcacctgcct gtgacatcat cccacaagat gaaatcatcc cccgggctca
      541 ctgcaggctc ctgtgcagtc aacagccctt tgaggtggtg gagaaggcct gccgctccta
      601 cagccacgtg gtgctcaagg aggtgcgctt cttcaacctg cagtccctct acccgctgct
      661 gaaagacccc tccctcaacc tgcatatcgt gcacctggtc cgggaccccc gggccgtgtt
      721 ccgttcccga gaacgcacaa agggagatct catgattgac agtcgcattg tgatggggca
      781 gcatgagcaa aaactcaaga aggaggacca accctactat gtgatgcagg tcatctgcca
      841 aagccagctg gagatctaca agaccatcca gtccttgccc aaggccctgc aggaacgcta
      901 cctgcttgtg cgctatgagg acctggctcg agcccctgtg gcccagactt cccgaatgta
      961 tgaattcgtg ggattggaat tcttgcccca tcttcagacc tgggtgcata acatcacccg
     1021 aggcaagggc atgggtgacc acgctttcca cacaaatgcc agggatgccc ttaatgtctc
     1081 ccaggcttgg cgctggtctt tgccctatga aaaggtttct cgacttcaga aagcctgtgg
     1141 cgatgccatg aatttgctgg gctaccgcca cgtcagatct gaacaagaac agagaaacct
     1201 gttgctggat cttctgtcta cctggactgt ccctgagcaa atccactaag agggttgaga
     1261 aggctttgct gccacctggt gtcagcctca gtcactttct ctgaatgctt ctgagccttg
     1321 cctacatctc tgagccttaa ctacatgtct gtgggtatca cactgagtgt gagttgtgtc
     1381 cacacgtgct caagcagaag gacttttgtg tccatgcttg tgtctagaaa acagactggg
     1441 gaaccttatg tgagcagcac atcccaccag tgaaacaggg tattgctctt cttcttttct
     1501 tgatcttcct gtctgggcag acttcagaga ctttgtggcc tggaggccta ttaagcacga
     1561 cacagtatca gtggaattga tccataaacc tccctgtcca catcttgccc aatggggaat
     1621 ggatctttca ccaaagagct caccagcatt ttccacagag atgcaaattc tgagcccttg
     1681 gagttcccag tggattcaag gaaggaagtg ggaacaaggt tggatgccta cttatgagct
     1741 tgaccatcac agctatcggt aatcagaaat atgaaacaaa atctctgcac aaaagagcaa
     1801 gctcttaagt tcacagggtg cctgggctgc atttgaatat cacttcccct ctgcattttc
     1861 ccatcacata gaagactttg acctgtgaag ctgccatctg ttaatactaa aattcccaaa
     1921 taaggttctg tttagaatgt ccctttttat gcttcttaat tattagcagt aaatgttcat
     1981 ttttatggga tcctaagcta agaatagatg taatcatctt tattggtttt ttaaaacacc
     2041 tttgctatta tctatcaccc atttatacag aaaattgcta agataatatt tggtttcaga
     2101 tgatgaacta agtctatacc ctcttaagaa ttggtggaaa atagacaatt agcagctaa
//
