LOCUS       NM_004267               4142 bp    mRNA    linear   PRI 06-JUN-2024
DEFINITION  Homo sapiens carbohydrate sulfotransferase 2 (CHST2), mRNA.
ACCESSION   NM_004267
VERSION     NM_004267.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4142)
  AUTHORS   Huang,K., Li,C., Zong,G., Prabhu,S.K., Chapla,D.G., Moremen,K.W.
            and Wang,L.X.
  TITLE     Site-selective sulfation of N-glycans by human
            GlcNAc-6-O-sulfotransferase 1 (CHST2) and chemoenzymatic synthesis
            of sulfated antibody glycoforms
  JOURNAL   Bioorg Chem 128, 106070 (2022)
   PUBMED   35939855
  REMARK    GeneRIF: Site-selective sulfation of N-glycans by human
            GlcNAc-6-O-sulfotransferase 1 (CHST2) and chemoenzymatic synthesis
            of sulfated antibody glycoforms.
REFERENCE   2  (bases 1 to 4142)
  AUTHORS   Yu,S.Y., Hsiao,C.T., Izawa,M., Yusa,A., Ishida,H., Nakamura,S.,
            Yagi,H., Kannagi,R. and Khoo,K.H.
  TITLE     Distinct substrate specificities of human
            GlcNAc-6-sulfotransferases revealed by mass spectrometry-based
            sulfoglycomic analysis
  JOURNAL   J Biol Chem 293 (39), 15163-15177 (2018)
   PUBMED   30093410
  REMARK    GeneRIF: mRNA transcript analyses on the genes involved in
            synthesizing GlcNAc-6-O-sulfated glycans in human colon cancer
            tissues indicated that GlcNAc6ST-2 (CHST4) is preferentially
            expressed in cancer cells ...  GlcNAc6ST-3 (CHST5) was only
            expressed in nonmalignant epithelial cells, whereas GlcNAc6ST-1
            (CHST2) was expressed equally in both cancerous and nonmalignant
            epithelial cells.
REFERENCE   3  (bases 1 to 4142)
  AUTHORS   Zhang,Z., Takeda-Uchimura,Y., Foyez,T., Ohtake-Niimi,S., Narentuya,
            Akatsu,H., Nishitsuji,K., Michikawa,M., Wyss-Coray,T., Kadomatsu,K.
            and Uchimura,K.
  TITLE     Deficiency of a sulfotransferase for sialic acid-modified glycans
            mitigates Alzheimer's pathology
  JOURNAL   Proc Natl Acad Sci U S A 114 (14), E2947-E2954 (2017)
   PUBMED   28320965
  REMARK    GeneRIF: GlcNAc6ST1 is up-regulated in the brains of Alzheimer's
            disease patients.
REFERENCE   4  (bases 1 to 4142)
  AUTHORS   Wu,Z.L., Robey,M.T., Tatge,T., Lin,C., Leymarie,N., Zou,Y. and
            Zaia,J.
  TITLE     Detecting O-GlcNAc using in vitro sulfation
  JOURNAL   Glycobiology 24 (8), 740-747 (2014)
   PUBMED   24799377
  REMARK    GeneRIF: A method for O-GlcNAc detection using in vitro sulfation
            with two N-acetylglucosamine (GlcNAc)-specific sulfotransferases,
            carbohydrate sulfotransferase 2 and carbohydrate sulfotransferase
            4.
REFERENCE   5  (bases 1 to 4142)
  AUTHORS   Desko,M.M., Gross,D.A. and Kohler,J.J.
  TITLE     Effects of N-glycosylation on the activity and localization of
            GlcNAc-6-sulfotransferase 1
  JOURNAL   Glycobiology 19 (10), 1068-1077 (2009)
   PUBMED   19571171
  REMARK    GeneRIF: report the sites at which
            N-Acetylglucosamine-6-sulfotransferase-1 is modified with N-linked
            glycans and the effects that each glycan has on enzyme activity,
            specificity, and
            localization.[N-Acetylglucosamine-6-sulfotransferase-1]
REFERENCE   6  (bases 1 to 4142)
  AUTHORS   Sakaguchi,H., Kitagawa,H. and Sugahara,K.
  TITLE     Functional expression and genomic structure of human
            N-acetylglucosamine-6-O-sulfotransferase that transfers sulfate to
            beta-N-acetylglucosamine at the nonreducing end of an
            N-acetyllactosamine sequence
  JOURNAL   Biochim Biophys Acta 1523 (2-3), 269-276 (2000)
   PUBMED   11042394
REFERENCE   7  (bases 1 to 4142)
  AUTHORS   Tu,L., Murphy,P.G., Li,X. and Tedder,T.F.
  TITLE     L-selectin ligands expressed by human leukocytes are HECA-452
            antibody-defined carbohydrate epitopes preferentially displayed by
            P-selectin glycoprotein ligand-1
  JOURNAL   J Immunol 163 (9), 5070-5078 (1999)
   PUBMED   10528213
REFERENCE   8  (bases 1 to 4142)
  AUTHORS   Li,X. and Tedder,T.F.
  TITLE     CHST1 and CHST2 sulfotransferases expressed by human vascular
            endothelial cells: cDNA cloning, expression, and chromosomal
            localization
  JOURNAL   Genomics 55 (3), 345-347 (1999)
   PUBMED   10049591
REFERENCE   9  (bases 1 to 4142)
  AUTHORS   Uchimura,K., Muramatsu,H., Kaname,T., Ogawa,H., Yamakawa,T.,
            Fan,Q.W., Mitsuoka,C., Kannagi,R., Habuchi,O., Yokoyama,I.,
            Yamamura,K., Ozaki,T., Nakagawara,A., Kadomatsu,K. and Muramatsu,T.
  TITLE     Human N-acetylglucosamine-6-O-sulfotransferase involved in the
            biosynthesis of 6-sulfo sialyl Lewis X: molecular cloning,
            chromosomal mapping, and expression in various organs and tumor
            cells
  JOURNAL   J Biochem 124 (3), 670-678 (1998)
   PUBMED   9722682
REFERENCE   10 (bases 1 to 4142)
  AUTHORS   Uchimura,K., Muramatsu,H., Kadomatsu,K., Fan,Q.W., Kurosawa,N.,
            Mitsuoka,C., Kannagi,R., Habuchi,O. and Muramatsu,T.
  TITLE     Molecular cloning and characterization of an
            N-acetylglucosamine-6-O-sulfotransferase
  JOURNAL   J Biol Chem 273 (35), 22577-22583 (1998)
   PUBMED   9712885
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC018450.26, BX387933.2,
            BC042160.1 and CA431595.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_004267.4.
            
            Summary: This locus encodes a sulfotransferase protein. The encoded
            enzyme catalyzes the sulfation of a nonreducing N-acetylglucosamine
            residue, and may play a role in biosynthesis of 6-sulfosialyl Lewis
            X antigen. [provided by RefSeq, Aug 2011].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB021124.1, SRR1163658.462526.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1966682, SAMEA1968189
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000309575.5/ ENSP00000307911.3
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-8                 AC018450.26        73084-73091         c
            9-53                BX387933.2         4-48
            54-1767             BC042160.1         1-1714
            1768-3086           BC042160.1         1716-3034
            3087-3970           AC018450.26        69020-69903         c
            3971-4142           CA431595.1         18-189              c
FEATURES             Location/Qualifiers
     source          1..4142
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3q24"
     gene            1..4142
                     /gene="CHST2"
                     /gene_synonym="C6ST; glcNAc6ST-1; Gn6ST-1; GST-2; GST2;
                     HEL-S-75"
                     /note="carbohydrate sulfotransferase 2"
                     /db_xref="GeneID:9435"
                     /db_xref="HGNC:HGNC:1970"
                     /db_xref="MIM:603798"
     exon            1..771
                     /gene="CHST2"
                     /gene_synonym="C6ST; glcNAc6ST-1; Gn6ST-1; GST-2; GST2;
                     HEL-S-75"
                     /inference="alignment:Splign:2.1.0"
     exon            772..4142
                     /gene="CHST2"
                     /gene_synonym="C6ST; glcNAc6ST-1; Gn6ST-1; GST-2; GST2;
                     HEL-S-75"
                     /inference="alignment:Splign:2.1.0"
     CDS             945..2537
                     /gene="CHST2"
                     /gene_synonym="C6ST; glcNAc6ST-1; Gn6ST-1; GST-2; GST2;
                     HEL-S-75"
                     /EC_number="2.8.2.-"
                     /note="carbohydrate (chondroitin 6/keratan)
                     sulfotransferase 2; N-acetylglucosamine
                     6-O-sulfotransferase 1; carbohydrate
                     (N-acetylglucosamine-6-O) sulfotransferase 2;
                     galactose/N-acetylglucosamine/N-acetylglucosamine
                     6-O-sulfotransferase 2; epididymis secretory protein Li
                     75"
                     /codon_start=1
                     /product="carbohydrate sulfotransferase 2"
                     /protein_id="NP_004258.2"
                     /db_xref="CCDS:CCDS3129.1"
                     /db_xref="GeneID:9435"
                     /db_xref="HGNC:HGNC:1970"
                     /db_xref="MIM:603798"
                     /translation="MSRSPQRALPPGALPRLLQAAPAAAPRALLPQWPRRPGRRWPAS
                     PLGMKVFRRKALVLCAGYALLLVLTMLNLLDYKWHKEPLQQCNPDGPLGAAAGAAGGS
                     WGRPGPPPAGPPRAHARLDLRTPYRPPAAAVGAAPAAAAGMAGVAAPPGNGTRGTGGV
                     GDKRQLVYVFTTWRSGSSFFGELFNQNPEVFFLYEPVWHVWQKLYPGDAVSLQGAARD
                     MLSALYRCDLSVFQLYSPAGSGGRNLTTLGIFGAATNKVVCSSPLCPAYRKEVVGLVD
                     DRVCKKCPPQRLARFEEECRKYRTLVIKGVRVFDVAVLAPLLRDPALDLKVIHLVRDP
                     RAVASSRIRSRHGLIRESLQVVRSRDPRAHRMPFLEAAGHKLGAKKEGVGGPADYHAL
                     GAMEVICNSMAKTLQTALQPPDWLQGHYLVVRYEDLVGDPVKTLRRVYDFVGLLVSPE
                     MEQFALNMTSGSGSSSKPFVVSARNATQAANAWRTALTFQQIKQVEEFCYQPMAVLGY
                     ERVNSPEEVKDLSKTLLRKPRL"
     regulatory      2602..2607
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CHST2"
                     /gene_synonym="C6ST; glcNAc6ST-1; Gn6ST-1; GST-2; GST2;
                     HEL-S-75"
                     /note="hexamer: AATAAA"
     polyA_site      2624
                     /gene="CHST2"
                     /gene_synonym="C6ST; glcNAc6ST-1; Gn6ST-1; GST-2; GST2;
                     HEL-S-75"
     regulatory      3066..3071
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CHST2"
                     /gene_synonym="C6ST; glcNAc6ST-1; Gn6ST-1; GST-2; GST2;
                     HEL-S-75"
                     /note="hexamer: AATAAA"
     regulatory      3082..3087
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CHST2"
                     /gene_synonym="C6ST; glcNAc6ST-1; Gn6ST-1; GST-2; GST2;
                     HEL-S-75"
                     /note="hexamer: AATAAT"
     polyA_site      3088
                     /gene="CHST2"
                     /gene_synonym="C6ST; glcNAc6ST-1; Gn6ST-1; GST-2; GST2;
                     HEL-S-75"
                     /note="major polyA site"
     polyA_site      3098
                     /gene="CHST2"
                     /gene_synonym="C6ST; glcNAc6ST-1; Gn6ST-1; GST-2; GST2;
                     HEL-S-75"
                     /note="major polyA site"
     regulatory      4120..4125
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CHST2"
                     /gene_synonym="C6ST; glcNAc6ST-1; Gn6ST-1; GST-2; GST2;
                     HEL-S-75"
                     /note="hexamer: AATACA"
     polyA_site      4142
                     /gene="CHST2"
                     /gene_synonym="C6ST; glcNAc6ST-1; Gn6ST-1; GST-2; GST2;
                     HEL-S-75"
ORIGIN      
        1 gcagaggctg gtccgagcgc gttgcgggcg cccggcgtaa ggggagtcgg gtagcagcat
       61 cctcctgggc gccgctttcg cgcggcggcg gcggctgcgg cggggtcttt ctttgcttaa
      121 atacctcgtt ggccagaagc gctggtaccg ggggcgggtt gggtcgggtc gggcagtgct
      181 gcacacctgg gtttccttgc ctagagctgt gtgttcgggg tcctttggtc cagtcggagg
      241 ctgcggagcg gcgggggttg cctgcgctgt ccgcccgggc atcctcccgg tgatggaagc
      301 agccgccgcc gccgctgcgg ggtcgcgctg tgccccatcc accgctgcca gagaggtggg
      361 aaaattcgcc gcacggaggc cgaaagcgag aggggctgcg ccgctatgcc gggagctgag
      421 tcccatataa gccgccccca gccatcgccc ccagccggct tcgttcccct gagcgagaca
      481 ggaagctgcg gtcccgagaa agcggaggag acgtcgctgg agccgggagg cgccgggttc
      541 ggcggagcgc ggagcggggc tctgggccgc gtgaaagttt ttcttcccga gccgcagggc
      601 gcccgctgcc cggaaactgc ccagggataa gtcggccgac tccccagacc cctcgaaggt
      661 gcggggaccc ccagcggaag cgagagggag cgaaatcgag gaacgagtga cagccggaca
      721 gtccgccggg cggtgatccg gggccgctcc cgggcgcgcc ctcggctcca ggtcctaccc
      781 ggagccgctg ccatgggaga gccagccttg ggcgctgggg accagccgcc gcgcccgcct
      841 cggagtcgcg gcccgagtcc cggcgccagc agccagcccg ctgcgtcccc ttcccgggct
      901 gcagggctgc ctccgccgcg ccgccggccc ggattgtgcc tgtgatgagc cgcagcccgc
      961 agcgagctct gcccccgggc gcgctccctc ggctgctcca ggctgcgcct gcagccgcgc
     1021 cgcgtgccct gctcccgcag tggccccggc gcccaggacg ccgctggccc gcgtcccctc
     1081 tcggaatgaa ggtgttccgt aggaaggcgc tggtgttgtg cgcgggctat gcactgctgc
     1141 tggtgctcac tatgctcaac ctcctggact acaagtggca caaggagccg ctgcagcagt
     1201 gcaaccccga tgggccgctg ggtgccgcag cgggggcagc cggaggcagc tgggggcgcc
     1261 cagggccgcc tccggccggg ccgccccgtg ctcatgcccg tttggacctc cgcactcctt
     1321 accgccctcc cgctgccgcc gtcggggcgg ctcctgcagc cgcggcaggg atggcggggg
     1381 ttgcggcccc tccaggcaat ggcactcggg gcaccggggg cgtcggggac aagcggcagc
     1441 tggtgtacgt gttcaccacg tggcgctctg gctcgtcgtt cttcggcgag ctattcaacc
     1501 agaatcccga ggtgttcttt ctctacgagc cagtgtggca tgtatggcaa aaactgtatc
     1561 cgggggacgc cgtttccctg cagggggcag cgcgggacat gctgagcgct ctttaccgct
     1621 gcgacctctc tgtcttccag ttgtatagcc ccgcgggcag cggggggcgc aacctcacca
     1681 cgctgggcat cttcggcgca gccaccaaca aggtggtgtg ctcgtcacca ctctgccccg
     1741 cctaccgcaa ggaggtcgtg gggttggtgg acgaccgcgt gtgcaagaag tgcccgccac
     1801 agcgcctggc gcgtttcgag gaggagtgcc gcaagtaccg cacactagtc ataaagggtg
     1861 tgcgcgtctt cgacgtggcg gtcttggcgc cactgctgcg agacccggcc ctggacctca
     1921 aggtcatcca cttggtgcgt gatccccgcg cggtggcgag ttcacggatc cgctcgcgcc
     1981 acggcctcat ccgtgagagc ctacaggtgg tgcgcagccg agacccgcga gctcaccgca
     2041 tgcccttctt ggaggccgcg ggccacaagc ttggcgccaa gaaggagggc gtgggcggcc
     2101 ccgcagacta ccacgctctg ggcgctatgg aggtcatctg caatagtatg gctaagacgc
     2161 tgcagacagc cctgcagccc cctgactggc tgcagggcca ctacctggtg gtgcggtacg
     2221 aggacctggt gggagacccc gtcaagacac tacggagagt gtacgatttt gtgggactgt
     2281 tggtgagccc cgaaatggag cagtttgccc tgaacatgac cagtggctcg ggctcctcct
     2341 ccaagccttt cgtggtatct gcacgcaatg ccacgcaggc cgccaatgcc tggcggaccg
     2401 ccctcacctt ccagcagatc aaacaggtgg aggagttttg ctaccagccc atggccgtcc
     2461 tgggctatga gcgggtcaac agccctgagg aggtcaaaga cctcagcaag accctgcttc
     2521 ggaagccccg tctctaaaag gggttcccag gagacctgat tccctgtggt gatacctata
     2581 aagaggatcg tagtgtgttt aaataaacac agtccagact caaacggagg aagcccacat
     2641 attctattat agatatataa ataatcacac acacacttgc tgtcaatgtt ttgagtcagt
     2701 gcatttcaag gaacagccac aaaatacaca cccctaagaa aaggcaagac ttgaacgttc
     2761 tgaccaggtg cccctcttct tctttgcctt ctcttgtcct ctttctccta tttcttaccc
     2821 tgtcctccac ctgccttcca ttttgaagtg ggatgttaat gaaatcaagt tccagtaacc
     2881 caaatcttgt ttacaaaata ttcgtggtat ctgtgaacat gttaagagta atttggatgt
     2941 gggggtgggg gtggagaaag gggaagtggt ccagaaacaa aaagccccat tgggcatgat
     3001 aagccgagga ggcattcttc taaagcagac ttttgtgtaa aaagcaaagg ttacatgtga
     3061 gtattaataa agaagataat aaataatatt ctttttaata tttgcctcca ttactttgga
     3121 ttcacctgtg ttacttgtcc tcagcttcag atagaatctc cttcccctcc ctcgtttcca
     3181 tttttccagt ctctttcctg aaaggcagct aaactgggtg ggagcaattt ttgcctcctt
     3241 ggtaataacc tgggggatgt tttctcacat gtaaatctca gcctgttagg tgtccaggag
     3301 tgaaacacat ctttgtattc taaaggcaga aaccaaacag ggcagtggaa gaaaaagcac
     3361 actactctct tcaactttga acacaggaag aagcaactcc ttcaggactt gaaggcattt
     3421 ggtttttcat tcctcttcag ttaaaatggg gaaccttgaa gcagagacca atgttttggt
     3481 gctgaggctg gttcagaaaa aggattttta aaaaaagtat gtaattttta aaagttctga
     3541 tgattagaac acagacctca ggaaagtagc gtgaacatac tgctggcgat ggtagcagct
     3601 tcgttggttt agcaaagtga cagaagtatc tatttggagt gtttttctga ccctgacacg
     3661 gtatgtggag gtggatgaaa gcagcgaagt ttcatctgag aaccgtaagg gttttccctt
     3721 ttcttacttg cttcccattt aaatcagtgc aagagagaat atgaatttat aatgctttac
     3781 ttgggatgcc tgtggaatat gttgcttttc tttatcatgt ttcctaaaag taaatttgca
     3841 aagaaagtaa catgctgcag acagtataag gcttcagcca agagcttctt ttagatatga
     3901 taatgaattg tggtaaagag gaaatgaaaa tgaactagtg tgtaggattt tgaaatttgg
     3961 tattttccag ggagaaaacc gttacatttt cctgtagtgg ttatgatgtt gtgtcctgaa
     4021 cccatttggt actgaaacat tctgcagaat gttatactat gagaagaaat tgtgaagccc
     4081 aaatgggaac aagagacagt gtagttgtat atgacagtaa atacagtaca agttcactat
     4141 ca
//
