LOCUS       NM_018641              15985 bp    mRNA    linear   PRI 02-APR-2024
DEFINITION  Homo sapiens carbohydrate sulfotransferase 12 (CHST12), transcript
            variant 3, mRNA.
ACCESSION   NM_018641
VERSION     NM_018641.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 15985)
  AUTHORS   Wang,J., Xia,X., Tao,X., Zhao,P. and Deng,C.
  TITLE     Knockdown of carbohydrate sulfotransferase 12 decreases the
            proliferation and mobility of glioblastoma cells via the
            WNT/beta-catenin pathway
  JOURNAL   Bioengineered 12 (1), 3934-3946 (2021)
   PUBMED   34288811
  REMARK    GeneRIF: Knockdown of carbohydrate sulfotransferase 12 decreases
            the proliferation and mobility of glioblastoma cells via the
            WNT/beta-catenin pathway.
REFERENCE   2  (bases 1 to 15985)
  AUTHORS   Lei,J., Yan,S., Zhou,Y., Wang,L., Zhang,J., Guo,X., Lammi,M.J.,
            Han,J. and Qu,C.
  TITLE     Abnormal expression of chondroitin sulfate sulfotransferases in the
            articular cartilage of pediatric patients with Kashin-Beck disease
  JOURNAL   Histochem Cell Biol 153 (3), 153-164 (2020)
   PUBMED   31845005
  REMARK    GeneRIF: Abnormal expression of chondroitin sulfate
            sulfotransferases in the articular cartilage of pediatric patients
            with Kashin-Beck disease.
REFERENCE   3  (bases 1 to 15985)
  AUTHORS   Zhou,H., Li,Y., Song,X., Zhao,Y., Cheng,L., Zhao,L. and Jia,L.
  TITLE     CHST11/13 Regulate the Metastasis and Chemosensitivity of Human
            Hepatocellular Carcinoma Cells Via Mitogen-Activated Protein Kinase
            Pathway
  JOURNAL   Dig Dis Sci 61 (7), 1972-1985 (2016)
   PUBMED   26993826
  REMARK    GeneRIF: These results have demonstrated that CHST11 and CHST13
            negatively modulate metastasis and drug resistance of HCC cells
            probably via oncogenic MAPK signal pathway.
REFERENCE   4  (bases 1 to 15985)
  AUTHORS   Sawcer,S., Hellenthal,G., Pirinen,M., Spencer,C.C.,
            Patsopoulos,N.A., Moutsianas,L., Dilthey,A., Su,Z., Freeman,C.,
            Hunt,S.E., Edkins,S., Gray,E., Booth,D.R., Potter,S.C., Goris,A.,
            Band,G., Oturai,A.B., Strange,A., Saarela,J., Bellenguez,C.,
            Fontaine,B., Gillman,M., Hemmer,B., Gwilliam,R., Zipp,F.,
            Jayakumar,A., Martin,R., Leslie,S., Hawkins,S., Giannoulatou,E.,
            D'alfonso,S., Blackburn,H., Martinelli Boneschi,F., Liddle,J.,
            Harbo,H.F., Perez,M.L., Spurkland,A., Waller,M.J., Mycko,M.P.,
            Ricketts,M., Comabella,M., Hammond,N., Kockum,I., McCann,O.T.,
            Ban,M., Whittaker,P., Kemppinen,A., Weston,P., Hawkins,C.,
            Widaa,S., Zajicek,J., Dronov,S., Robertson,N., Bumpstead,S.J.,
            Barcellos,L.F., Ravindrarajah,R., Abraham,R., Alfredsson,L.,
            Ardlie,K., Aubin,C., Baker,A., Baker,K., Baranzini,S.E.,
            Bergamaschi,L., Bergamaschi,R., Bernstein,A., Berthele,A.,
            Boggild,M., Bradfield,J.P., Brassat,D., Broadley,S.A., Buck,D.,
            Butzkueven,H., Capra,R., Carroll,W.M., Cavalla,P., Celius,E.G.,
            Cepok,S., Chiavacci,R., Clerget-Darpoux,F., Clysters,K., Comi,G.,
            Cossburn,M., Cournu-Rebeix,I., Cox,M.B., Cozen,W., Cree,B.A.,
            Cross,A.H., Cusi,D., Daly,M.J., Davis,E., de Bakker,P.I.,
            Debouverie,M., D'hooghe,M.B., Dixon,K., Dobosi,R., Dubois,B.,
            Ellinghaus,D., Elovaara,I., Esposito,F., Fontenille,C., Foote,S.,
            Franke,A., Galimberti,D., Ghezzi,A., Glessner,J., Gomez,R.,
            Gout,O., Graham,C., Grant,S.F., Guerini,F.R., Hakonarson,H.,
            Hall,P., Hamsten,A., Hartung,H.P., Heard,R.N., Heath,S., Hobart,J.,
            Hoshi,M., Infante-Duarte,C., Ingram,G., Ingram,W., Islam,T.,
            Jagodic,M., Kabesch,M., Kermode,A.G., Kilpatrick,T.J., Kim,C.,
            Klopp,N., Koivisto,K., Larsson,M., Lathrop,M., Lechner-Scott,J.S.,
            Leone,M.A., Leppa,V., Liljedahl,U., Bomfim,I.L., Lincoln,R.R.,
            Link,J., Liu,J., Lorentzen,A.R., Lupoli,S., Macciardi,F., Mack,T.,
            Marriott,M., Martinelli,V., Mason,D., McCauley,J.L., Mentch,F.,
            Mero,I.L., Mihalova,T., Montalban,X., Mottershead,J., Myhr,K.M.,
            Naldi,P., Ollier,W., Page,A., Palotie,A., Pelletier,J., Piccio,L.,
            Pickersgill,T., Piehl,F., Pobywajlo,S., Quach,H.L., Ramsay,P.P.,
            Reunanen,M., Reynolds,R., Rioux,J.D., Rodegher,M., Roesner,S.,
            Rubio,J.P., Ruckert,I.M., Salvetti,M., Salvi,E., Santaniello,A.,
            Schaefer,C.A., Schreiber,S., Schulze,C., Scott,R.J., Sellebjerg,F.,
            Selmaj,K.W., Sexton,D., Shen,L., Simms-Acuna,B., Skidmore,S.,
            Sleiman,P.M., Smestad,C., Sorensen,P.S., Sondergaard,H.B.,
            Stankovich,J., Strange,R.C., Sulonen,A.M., Sundqvist,E.,
            Syvanen,A.C., Taddeo,F., Taylor,B., Blackwell,J.M., Tienari,P.,
            Bramon,E., Tourbah,A., Brown,M.A., Tronczynska,E., Casas,J.P.,
            Tubridy,N., Corvin,A., Vickery,J., Jankowski,J., Villoslada,P.,
            Markus,H.S., Wang,K., Mathew,C.G., Wason,J., Palmer,C.N.,
            Wichmann,H.E., Plomin,R., Willoughby,E., Rautanen,A.,
            Winkelmann,J., Wittig,M., Trembath,R.C., Yaouanq,J.,
            Viswanathan,A.C., Zhang,H., Wood,N.W., Zuvich,R., Deloukas,P.,
            Langford,C., Duncanson,A., Oksenberg,J.R., Pericak-Vance,M.A.,
            Haines,J.L., Olsson,T., Hillert,J., Ivinson,A.J., De Jager,P.L.,
            Peltonen,L., Stewart,G.J., Hafler,D.A., Hauser,S.L., McVean,G.,
            Donnelly,P. and Compston,A.
  CONSRTM   International Multiple Sclerosis Genetics Consortium; Wellcome
            Trust Case Control Consortium 2
  TITLE     Genetic risk and a primary role for cell-mediated immune mechanisms
            in multiple sclerosis
  JOURNAL   Nature 476 (7359), 214-219 (2011)
   PUBMED   21833088
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 15985)
  AUTHORS   Valente,B., Campos,P.A., do Rosario,V.E. and Silveira,H.
  TITLE     Natural frequency of polymorphisms linked to the chondroitin
            4-sulfotransferase genes and its association with placental malaria
  JOURNAL   Trans R Soc Trop Med Hyg 104 (10), 687-689 (2010)
   PUBMED   20580388
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   6  (bases 1 to 15985)
  AUTHORS   Ghosh,D., Lippert,D., Krokhin,O., Cortens,J.P. and Wilkins,J.A.
  TITLE     Defining the membrane proteome of NK cells
  JOURNAL   J Mass Spectrom 45 (1), 1-25 (2010)
   PUBMED   19946888
REFERENCE   7  (bases 1 to 15985)
  AUTHORS   Clark,H.F., Gurney,A.L., Abaya,E., Baker,K., Baldwin,D., Brush,J.,
            Chen,J., Chow,B., Chui,C., Crowley,C., Currell,B., Deuel,B.,
            Dowd,P., Eaton,D., Foster,J., Grimaldi,C., Gu,Q., Hass,P.E.,
            Heldens,S., Huang,A., Kim,H.S., Klimowski,L., Jin,Y., Johnson,S.,
            Lee,J., Lewis,L., Liao,D., Mark,M., Robbie,E., Sanchez,C.,
            Schoenfeld,J., Seshagiri,S., Simmons,L., Singh,J., Smith,V.,
            Stinson,J., Vagts,A., Vandlen,R., Watanabe,C., Wieand,D., Woods,K.,
            Xie,M.H., Yansura,D., Yi,S., Yu,G., Yuan,J., Zhang,M., Zhang,Z.,
            Goddard,A., Wood,W.I., Godowski,P. and Gray,A.
  TITLE     The secreted protein discovery initiative (SPDI), a large-scale
            effort to identify novel human secreted and transmembrane proteins:
            a bioinformatics assessment
  JOURNAL   Genome Res 13 (10), 2265-2270 (2003)
   PUBMED   12975309
  REMARK    Erratum:[Genome Res. 2003 Dec;13(12):2759]
REFERENCE   8  (bases 1 to 15985)
  AUTHORS   Mikami,T., Mizumoto,S., Kago,N., Kitagawa,H. and Sugahara,K.
  TITLE     Specificities of three distinct human chondroitin/dermatan
            N-acetylgalactosamine 4-O-sulfotransferases demonstrated using
            partially desulfated dermatan sulfate as an acceptor: implication
            of differential roles in dermatan sulfate biosynthesis
  JOURNAL   J Biol Chem 278 (38), 36115-36127 (2003)
   PUBMED   12847091
  REMARK    GeneRIF: human D4ST-1, C4ST-1, and S4ST-2 have differential roles
            in dermatan sulfate biosynthesis
REFERENCE   9  (bases 1 to 15985)
  AUTHORS   Hiraoka,N., Nakagawa,H., Ong,E., Akama,T.O., Fukuda,M.N. and
            Fukuda,M.
  TITLE     Molecular cloning and expression of two distinct human chondroitin
            4-O-sulfotransferases that belong to the HNK-1 sulfotransferase
            gene family
  JOURNAL   J Biol Chem 275 (26), 20188-20196 (2000)
   PUBMED   10781601
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA704587.1, AY358574.1,
            AC004840.4 and AF239822.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_018641.4.
            
            Summary: The protein encoded by this gene belongs to the
            sulfotransferase 2 family. It is localized to the golgi membrane,
            and catalyzes the transfer of sulfate to position 4 of the
            N-acetylgalactosamine (GalNAc) residue of chondroitin and
            desulfated dermatan sulfate. Chondroitin sulfate constitutes the
            predominant proteoglycan present in cartilage, and is distributed
            on the surfaces of many cells and extracellular matrices.
            Alternatively spliced transcript variants differing only in their
            5' UTRs have been found for this gene. [provided by RefSeq, Aug
            2011].
            
            Transcript Variant: This variant (3) uses an alternate donor splice
            site at the 5' terminal non-coding exon, hence has a slightly
            longer 5' UTR compared to variant 1. Variants 1-3 encode the same
            protein.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR3476690.372028.1,
                                           SRR3476690.1045687.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2142670, SAMEA2142680
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000618655.2/ ENSP00000481912.1
            RefSeq Select criteria :: based on conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-13                DA704587.1         52-64
            14-56               AY358574.1         1-43
            57-57               AC004840.4         110127-110127       c
            58-1560             AY358574.1         45-1547
            1561-2108           AF239822.1         1523-2070
            2109-15985          AC004840.4         65310-79186         c
FEATURES             Location/Qualifiers
     source          1..15985
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7p22.3"
     gene            1..15985
                     /gene="CHST12"
                     /gene_synonym="C4S-2; C4ST-2; C4ST2"
                     /note="carbohydrate sulfotransferase 12"
                     /db_xref="GeneID:55501"
                     /db_xref="HGNC:HGNC:17423"
                     /db_xref="MIM:610129"
     exon            1..63
                     /gene="CHST12"
                     /gene_synonym="C4S-2; C4ST-2; C4ST2"
                     /inference="alignment:Splign:2.1.0"
     exon            64..15985
                     /gene="CHST12"
                     /gene_synonym="C4S-2; C4ST-2; C4ST2"
                     /inference="alignment:Splign:2.1.0"
     CDS             141..1385
                     /gene="CHST12"
                     /gene_synonym="C4S-2; C4ST-2; C4ST2"
                     /EC_number="2.8.2.5"
                     /note="chondroitin 4-O-sulfotransferase 2;
                     sulfotransferase Hlo; chondroitin 4-sulfotransferase 2;
                     carbohydrate (chondroitin 4) sulfotransferase 12"
                     /codon_start=1
                     /product="carbohydrate sulfotransferase 12"
                     /protein_id="NP_061111.1"
                     /db_xref="CCDS:CCDS5333.1"
                     /db_xref="GeneID:55501"
                     /db_xref="HGNC:HGNC:17423"
                     /db_xref="MIM:610129"
                     /translation="MTKARLFRLWLVLGSVFMILLIIVYWDSAGAAHFYLHTSFSRPH
                     TGPPLPTPGPDRDRELTADSDVDEFLDKFLSAGVKQSDLPRKETEQPPAPGSMEESVR
                     GYDWSPRDARRSPDQGRQQAERRSVLRGFCANSSLAFPTKERAFDDIPNSELSHLIVD
                     DRHGAIYCYVPKVACTNWKRVMIVLSGSLLHRGAPYRDPLRIPREHVHNASAHLTFNK
                     FWRRYGKLSRHLMKVKLKKYTKFLFVRDPFVRLISAFRSKFELENEEFYRKFAVPMLR
                     LYANHTSLPASAREAFRAGLKVSFANFIQYLLDPHTEKLAPFNEHWRQVYRLCHPCQI
                     DYDFVGKLETLDEDAAQLLQLLQVDRQLRFPPSYRNRTASSWEEDWFAKIPLAWRQQL
                     YKLYEADFVLFGYPKPENLLRD"
     regulatory      1536..1541
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CHST12"
                     /gene_synonym="C4S-2; C4ST-2; C4ST2"
                     /note="hexamer: AATATA"
     polyA_site      1562
                     /gene="CHST12"
                     /gene_synonym="C4S-2; C4ST-2; C4ST2"
                     /note="major polyA site"
     regulatory      15692..15697
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CHST12"
                     /gene_synonym="C4S-2; C4ST-2; C4ST2"
                     /note="hexamer: AATAAA"
     polyA_site      15713
                     /gene="CHST12"
                     /gene_synonym="C4S-2; C4ST-2; C4ST2"
     regulatory      15963..15968
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CHST12"
                     /gene_synonym="C4S-2; C4ST-2; C4ST2"
                     /note="hexamer: AGTAAA"
     polyA_site      15985
                     /gene="CHST12"
                     /gene_synonym="C4S-2; C4ST-2; C4ST2"
ORIGIN      
        1 agtcgcgggg cggcggcggc ggctgcgggc gcgaggtgag gggcgcgagg tgagggtcgc
       61 gaggttccca gcaggatgcc ccggctctgc aggaagctga agtgagaggc ccggagaggg
      121 cccagcccgc ccggggcagg atgaccaagg cccggctgtt ccggctgtgg ctggtgctgg
      181 ggtcggtgtt catgatcctg ctgatcatcg tgtactggga cagcgcaggc gccgcgcact
      241 tctacttgca cacgtccttc tctaggccgc acacggggcc gccgctgccc acgcccgggc
      301 cggacaggga cagggagctc acggccgact ccgatgtcga cgagtttctg gacaagtttc
      361 tcagtgctgg cgtgaagcag agcgaccttc ccagaaagga gacggagcag ccgcctgcgc
      421 cggggagcat ggaggagagc gtgagaggct acgactggtc cccgcgcgac gcccggcgca
      481 gcccagacca gggccggcag caggcggagc ggaggagcgt gctgcggggc ttctgcgcca
      541 actccagcct ggccttcccc accaaggagc gcgcattcga cgacatcccc aactcggagc
      601 tgagccacct gatcgtggac gaccggcacg gggccatcta ctgctacgtg cccaaggtgg
      661 cctgcaccaa ctggaagcgc gtgatgatcg tgctgagcgg aagcctgctg caccgcggtg
      721 cgccctaccg cgacccgctg cgcatcccgc gcgagcacgt gcacaacgcc agcgcgcacc
      781 tgaccttcaa caagttctgg cgccgctacg ggaagctctc ccgccacctc atgaaggtca
      841 agctcaagaa gtacaccaag ttcctcttcg tgcgcgaccc cttcgtgcgc ctgatctccg
      901 ccttccgcag caagttcgag ctggagaacg aggagttcta ccgcaagttc gccgtgccca
      961 tgctgcggct gtacgccaac cacaccagcc tgcccgcctc ggcgcgcgag gccttccgcg
     1021 ctggcctcaa ggtgtccttc gccaacttca tccagtacct gctggacccg cacacggaga
     1081 agctggcgcc cttcaacgag cactggcggc aggtgtaccg cctctgccac ccgtgccaga
     1141 tcgactacga cttcgtgggg aagctggaga ctctggacga ggacgccgcg cagctgctgc
     1201 agctactcca ggtggaccgg cagctccgct tccccccgag ctaccggaac aggaccgcca
     1261 gcagctggga ggaggactgg ttcgccaaga tccccctggc ctggaggcag cagctgtata
     1321 aactctacga ggccgacttt gttctcttcg gctaccccaa gcccgaaaac ctcctccgag
     1381 actgaaagct ttcgcgttgc tttttctcgc gtgcctggaa cctgacgcac gcgcactcca
     1441 gtttttttat gacctacgat tttgcaatct gggcttcttg ttcactccac tgcctctatc
     1501 cattgagtac tgtatcgata ttgtttttta agattaatat atttcaggta tttaatacga
     1561 aatgtggaag ggaatgctgg agtaaaatat cccctctccc ctccgcccgc ccacccgccc
     1621 gcccgctcgc ccgctcgccc gctcctgtgg tttttctgag cgtgcgggcg ccgggagggg
     1681 atgctgaggc tgatggagct gcctccaggg ctagggccac tcaccggagg agggcggggc
     1741 ctgcacttga agtcaggccg cacctgtctg tttttggaag ggtagccgac aaatccttcc
     1801 agagggaaag ttctttgttt aagtgttgta cttgaaaagg tcaatcttca gggcttcctg
     1861 tttgaagtca agtcagaggt aaaccggtca gttacagaag caggatttct aggatttcta
     1921 actccagctg ttcccatact gtctagttta aattatggct gttaaggccg ggcgggtgac
     1981 tcaggcaggt aatcccagaa ctttgggagg cccagacaga aggatcgctt gaggtcagga
     2041 atttgagacc tggccaacat ggtgaaaccc tgtctctact aaaaaaaaaa aaaaaaaaaa
     2101 aaaaaaaaaa aaaaaaatga gtcgggtgtg gtggtgtgca cctgtagtcc cagctactca
     2161 ggagttctga gatggaaaga ttgcttgaac ccaggaggca gaggctgcag tgagctgaga
     2221 tcgcgacatt gcactctagc ctgggtgaca gagtgagatt ccatctcaaa ataaataaat
     2281 aaatattaat acattatggc tattaaggct gggcacggtg gctcatgcct gcaatcctgg
     2341 cactttggga ggctgagggg ggaagatcac ttgagcccag gagttcaaga ccagcttggg
     2401 caacatagca aggacctcat ctctacaaaa aataaaaagg acgtggtggt gcacatctgt
     2461 aaacccagct actcaggagg ctgaggaggg aggatcactt gagcccagga gttcaagatc
     2521 agtttggacc acatagcgag accctgtgtc tacaaaaaat aagaaaatca gccggacatg
     2581 atggtgcaca cctgtaatcc cagctactcg ggaggctgag gcaggaggat cgctggagcc
     2641 caggagttca aggctgcagt gacctatgat cgtgccactg cactccagcc tgggcagcaa
     2701 agtgagactc tgtttctaaa tagacagata gaagataggt agatagatag attaaataga
     2761 aagtaaacta tggctgttga ggtttacagt tggcaggagt ggagtggttt ggcgtttatc
     2821 tctagaccgt gctggatgac agtacccagt gtcaccaaga agaggctccg tgtgactgtc
     2881 tctgtggctt tctggttggt ttagctccag tgccttcacc ctcaaggaca cgactgacct
     2941 tgaaccctca ggtttgatgg gccctgtctt gataaaagtg tcagaggagg ctctggaaac
     3001 atctggctga aactcctttg ctatttctgc ttctaagctg tagttggaga gatggttgca
     3061 atcatttcgt ggtgtcgggc accctgcttg acctgtcttc ctcgtgtgtg gtatttgtca
     3121 ccactgccca gtccctggtt gctggccacg ctcagttggg acataccctg gtgtcctctg
     3181 tgtgtggtgt ccctgtgttt cccaggctca ggtgggcttg tgtgggtctt ggcccccagt
     3241 gcctgctagc ctcaggtgtc ccctgtgtta gacacctcct gccttccttt ctgaagcttc
     3301 gctttctttt atttttgttt tgttttgttt ttggagacag ggtctcgctc tgtcatccgg
     3361 gctggaatac actggtgcaa tcacagctca ctgcagcttc aacctcctgg gctcaagtga
     3421 tcttcccatc tcagactccc aagtagctgg ggttacaggt gagcgccagc ctgcccagct
     3481 agttttttat tttcagcaga gatgaggtct cactgtgttg cccaggctgc tctcaaactc
     3541 ctgggctcaa gtgaccctcc tgcatcaccc tcccaaagtg ctgggattac aggcatgagc
     3601 catcacacct ggcccctcca agttttttat tatgataaaa atgtttaatt ttaaacgtac
     3661 catcttaacc attgttaagt gcactgttgt gtggcattaa gcacattcgt attgtcgtgc
     3721 caccaccacc atcatctcca gagctttctc atcttcccaa actgaaactc tgtccccatt
     3781 cagcactcgc tccccattgg tagaaggcag cagcttccac tctctgattt ctactacccg
     3841 agctccctca gaaatggagt cacacagtat tggtcctttt gggactgaag tacttcacct
     3901 catgcaatgt cctctaggtt catcggtgtc gtggcaggtg tcagaactaa cttcctttct
     3961 gaggcggaat aatgtcccat tgtgtggaag aggcatggtt tgtttatctg ttcatcagtt
     4021 ggtggacact gagttgcttc tgccttcctg cgaatgtgaa ttgaatgctg ctgtgaacag
     4081 ggtgtcagtg aatctgctga gggcttgttt tccgtattct gggcatggcc ctttgtgtct
     4141 gcctttgctg gagcacctcc ccgggatgcg tttgtttata ggatgttcag acgaatgtca
     4201 gaacccactg ggcggttcac tcactgaggg gggcccgcgt cccctccatt tctgcattcc
     4261 cagttcttgg tatggttcct ctgaggttcg gttttcctga gcacctgggg tgtttgcagg
     4321 gatgtggagg ggttcctgca gaagacacca cccacatcca cgcacctgct cagattcccg
     4381 ggccatggtc ggggccaccg cccgtgggga tgactccgga tgggagtggc agaaaagggc
     4441 ctgctgggcc gaggggaggc gtctccggga agagagggag gtgtgagcgg ggagaaccca
     4501 ctttgattct tcgctgtttg cctgatgcgc agcaccacac tgcgtagaga cctttggctc
     4561 tggttttttc tttttccact gaaacacagc aaattataca ttttatgaaa attggcatcg
     4621 tacaactgga aaatttaacc ataaacttaa aactctttta ccctgttggc ccccatcctc
     4681 cagctgtgtc cacactttga gcacagtcat gagaatgact tggttgggtg cggggtggtg
     4741 tcacaaagcc ctgtctcttg tgtgatggct gcgcctcgaa tcagtgggag ggcacctcgc
     4801 ccgtttacag ttgggcaatg cttgggctgg gcggcctggc tgctcctgga ctcgcctccc
     4861 agcgtctgat gctgtgtatt ttccatctgc cccaggaatt tgacacaggt gtgacttgca
     4921 ggttgtgtgg gtgatgcctc cctccctctt tcctcttaaa gcatctcatg cttcttcatt
     4981 cgctgatact tacaaccccc agtggatatt tagtggagtt tgctgtagtg cttttgccat
     5041 ttatttggac tattggaaaa atttcaggtt tggttttggc cctatgtctt gatggagggc
     5101 aagggttagg atttcagttt atcttcccgg tgaggtctga gcacgtctgt gccctgaggt
     5161 gggtggtttg gtgaaggtgg ggctttaacc tagctctgcc acttaggaat cagaacacac
     5221 acacacacgc gcgcacacac acacacacac acacactctc tctcacacac acatcgggat
     5281 atttttcttg tttccttctt ccagagggtg taggagttgt gttccaagtt tgttcctgca
     5341 aaccctccga tgaggccagc tgcattcatc ggggcctgga tggccgcacc tctctctccc
     5401 cgccctgtct cctgcactca ggtgagaggc tggcagggct gccgtagggg tggagagtga
     5461 acgcagggtg gctaagccct cgtccctgtg agggacagtg acacggatgg gagcagccat
     5521 ctgagctgga agctgtggac atggtccttg tgtgggtcca agcaaagtgg gtggcgtgtg
     5581 cattttgtga acacgtggtc tgcttcctgg tgcagaccag gtctccccag agggaccctc
     5641 ctgagctgag ctgcccgggg cacctgtccc tggatgtgaa ctgccttggt tgtaccttgg
     5701 tggggggacc tttcctgaat gtgttggttc ggcatggtga caggtgtttc cctgcggcag
     5761 aatgttgggg ccacaccaac gtccccagag tcttctccct agagtctctg aaaggaagcc
     5821 tgggttcctg gtggtgctcc cctccatgtg tgctcacccg cctgtatgga ggcagcccag
     5881 gggagcggct gagactcttg gcagagagct ggggacgctc tgttctcatt ggcagcccca
     5941 ccctgaagaa taacagactc ccaaatatca cacaggctgc agataccacc cagcccccaa
     6001 ggatgccatc accaggctgg ggcgctccca tgcccattat ccaggggtaa tttttgctca
     6061 cttatttata agccacacat cctcttctat cctaccaaac ctaagaatat caaaagggaa
     6121 agcttggctt gcatggacgt aattaaccta aaagtcttta aacgtttctc ccgagtgttg
     6181 gagtgactgt ttgcacacat ctgttgtgtt ttgcctgtgt gcattcctcc tcctggtcag
     6241 agagcccgtc tcttccgccc tctcccttac cctaggaggg ctgagtcagg cctccctacc
     6301 caggcccaag ggcagatgca cctgagccat tgtgtcccat ggttgatacg ggacggggct
     6361 gatttcctga cctggacccc tgagacccag tgctcttagc tgtgagcctc tgggaagagg
     6421 ttctggcagg tggaggggat ggacgttaca gtgctgtggc cacctagaaa agccgtcccg
     6481 gaactgagac caactgctga gggaggaagg gccaccagat cgggggccgg gccggggtga
     6541 gcacctgaag ccggctccac ctgctcttct cagctgtgcg cctccaggaa tttgcactcg
     6601 cttcagctga tttgggttgg atttctagcg tttcacatac aggatctaga ctcgcacaca
     6661 actgcattaa tatgttggtg tttgataaac tagggttgaa ggagttccat cagtgaagag
     6721 agaagccacg tgacctgagg atgcagggca ctgttcacgt ggacatgagc cgtttcttag
     6781 catcatgaga ttttagaaac atgttcttac tctgccagga cccagggata ggtgaggctc
     6841 tcctattggg ataccatttc accagcagag agattaattg gagaatgtgt aattttttcc
     6901 cctttggaaa taatttgttc tttttttttt ttgacagagt ttctttctgt catccaggct
     6961 ggagtgcagt ggtgtgatca tagctcactg cagcctcgac ctcctgggct caagtggtcc
     7021 tcccacctca gcctcccaag tagctgggac tacaggcatg caccaccatg cccggctaat
     7081 ttttacattt ttggtagagg ccgggtacgg tggctcatgc ctgtaatccc ggcactttgg
     7141 gaggccgagg tgggtggatc acgaggtcag gagatcaaga ccatcctgga taacatggtg
     7201 aaaccccgtc tctactaaaa tacaaaaaaa ttagccgggc gtggtggtgg gcgcctgtag
     7261 tcccagctac tcgggaggct gaggcaggag aatggcgtga acccgggagg cggagcttgc
     7321 agtgagcgga gattggcgcc actacactcc agcctgggcg acagagcgag actccgtctc
     7381 aaaaaaaaat tttttttttt ggtagatacg gggtcttgct ttgtcaccca ggctggtctc
     7441 aaactcctga gctcgagtgc tccttctgcc tcagcctccc aaagtgctgg gattacaggc
     7501 gtgggctgct gtgcccagca ggaataattt ctcagtgcat ctctgatggg gaggctaatg
     7561 aagaaaggac acctggtttg aaggcttctc tgtgtctgtc cgtgggcgct actgatctga
     7621 gccaagcacc attccgcagg catgcctgaa atgactccca tggctgtcat ggtgccatct
     7681 ggagagcatt tgctcactgt aatggtcccg tcacacttgt ctgatgagga tctgttggtt
     7741 gactttgaca gtgataaacg aacacagcct atctgggtgt tttttgcact tatagtttgg
     7801 gggtttgaag tactggctag aagctggcgg ggtgtgtgtg tgcaggtgtg cgagtgcatg
     7861 tgcaagtgtg cgtgcaagtg tgagtctgca cttgtgtgca agagaatgtg tgtgtgtgtg
     7921 tgtttggaga tttggacttg aaaattctag ctcaggtctt tggggtagtg tttgacctga
     7981 actctgaaag gcagagggtc ccagactttc ctgggatgat gggaactggg caccccagat
     8041 gaggctcggt tgtgagtagg agcggggtgg gccaccgaag agaagacaga gtgggcctgt
     8101 agggccagcc cgaggtaccc acagtggagg gtctacaggc tgatttggga ggtggtggtt
     8161 tatacctgta gtcaccttct atcttagtta ggaattcacc ttcacacttc cagaaactgc
     8221 caagaagaat gccgccgctc aggtttatct ggtagaataa gctgtttgaa caaagatctg
     8281 gaggttgcac aatcctgtgt cattctggat ttatcttggg gtcgggagtc aacgatactt
     8341 cttgtgctgt cgtttctccg gccgtgtgaa gttaccacct cgtgagtggt cataagttag
     8401 cgtgtctaaa tgcactttga aatcctagga tgaaaaagcc aggccctgtc ttttttcctt
     8461 gtctaagcac catccttgct taggagagac acggctgtgg ctctcaggct gtggggcaat
     8521 gttctgctta taatgtttca agaggttcaa agcgtgcagg ccccatgctc cgtgagagcc
     8581 ttgacctggg tcagcagggg cggctgggct tgactcgaga attcccacag ggcccgaggg
     8641 aagagcagag caagagggag gagcgcgtgg agccctatgt ggcctgcgat attggggtgg
     8701 cgtccaggcc cgcctagatg ggggagggag acaacagagc tgccacctgc tcaccgcaga
     8761 cccaggggcc caggaggagc ggactaggac cggcagtgcc ttccagccta ggtctcctgc
     8821 ccctgggctg gagttcatct tttaggcaaa tttggaatag gaaccttaaa aatgctttct
     8881 tcactcgttt aaatagtaat gataagaata tggctgcagg gggcagctta tgcctgtaat
     8941 cccaccattt tgggagctca aggcaggagt attgcttgag cccagaagtt tgagaccagt
     9001 ctgggcaaca tagtggaaac ttgtctctcc aaaagctaaa agaaaaagcc gtgcatggtg
     9061 gtatgcacct gtagtcccag ctacttggga ggctgaggtg ggaggatcgc ttgagtctgg
     9121 gaggtcgagg ctgctgtgag ccatgattgc accactgcat tccagcctgg gtgacagagc
     9181 aagatcttgt cttaaaaaaa aaaaaaaaaa aaaaggtgtt gtattttatc attagaaggc
     9241 catcctgttt agaagagtgg gtttctcata agaaaaaaaa gaaaaaggtt gaccttgtaa
     9301 gtatattttc ttgggtgaat tgtggtggtg gtaatggttt taactggtgt taatttcagt
     9361 tcttttaaaa gttgtggtaa gatacatgca acataaaatg tgccctcttc cccattttta
     9421 agtgaacagt tcagtggcat taagcacatt cagtttgcct gccaccgtta ccaccatcca
     9481 tctccaaaat tctctccatt tacaaaactg aaactctgtc cctattaaac actagctccc
     9541 cattccccac tgtgccgccc ccccaaagcc cctggcacca accattctac ttcctgtctc
     9601 tgagtttgac tagggacctc ctgtaagtgg atttgtacaa tatttgtcct tttgtgtctg
     9661 gcttcttcat gtagtataat gtcttcaagg ttcattcatg ttgtagtcag gatcaccttc
     9721 cttttaaagg ttgaacgata tcccgttgta tggacagacc acattttatc cattcaccca
     9781 gggatggaca cctgggttgc ttccatctct cggctcttgt gaataatgct gctgtgaaca
     9841 cgggtgtgca gatacctgtt caagtccctg ctgttttctt ttgagacgga gtctcgctct
     9901 gtctcccagg ctggagtgcg gtggcaccat ctcagctcac tgcaagctcc gcctcccagg
     9961 ttcaggccat tctcctgcct cagcctcccg agtagctggg actacaggtg cccaccacca
    10021 cgcctggcta attttttgta tttttagtag agacggagtt tcaccatgtt agccaggatg
    10081 ctctggatct cctgacctcg tgatccaccc acctcggcct cccaaactgc tgggattaca
    10141 ggcatgagac accacgcccc gccgagtccc tgcttttaat tctttggggc agggatcccc
    10201 aaccccctgg tgatggacca atactggcct gcggcccctt aggaactgga ctgcacagca
    10261 ggaggtgagc ggcaggtgag cgtgcattcc cgccggagcc ccgcctcccg tcagatcagc
    10321 ggcggcatta gattctcata ggagcgggaa ccctcctgtg aaccctgcat accagggttc
    10381 tccatgcaac aatctaatgc ctgatgatct gaagtggaac aggtttttgt tttttttttc
    10441 cagacagagt cttgctcttt cgcctaggct ggagtgcagt ggcatgatct cggctcactg
    10501 caacccctgc ttcctgggtt caagtggttc tcctgcctca gcctcccaag tagctgggat
    10561 tacaggtgcc cgccaccatg gccagctaat ttttttgttt atttgtttat ttattatttt
    10621 ttgaggtgga gtcttgctgt cacccaggct ggagtgcagt ggtgcaatct cagctcactg
    10681 caacctctgc cttctgggtt caagccattc ttgtgcctca gcttcccgag taggtggaat
    10741 tataggcgtg tgccaccatg cccagctaat ttttgtattt ttagtagagg cgggatttca
    10801 ccatgttggc caggctggtc tcactcttga cctcaagtga tctgcccgcc tcggcctccc
    10861 aaagtgttgg gattacaggt gtgagccacc gtgctcagcc tgaagtgaaa cagtttcatc
    10921 ccaaaagcat atacctctgc cccatccatg gaaaaattgc ctctcatgaa accagtccct
    10981 ggtgccaaaa aggttgggga ccgcggctct ggagtatatg cctaggagta ggagtgtggg
    11041 gtcatatggt aatttatctt taatttttgg aggaattgaa tttcaattct tattattacc
    11101 aaaaatactt aaaattctta cagatggcat gggaaagtag cacacagtcg ggactctgca
    11161 gccgagtttg cctccttgaa tgcacacata acacaggaac aaagacagag ctgcttccct
    11221 cctgttgggt ctctggcgga cagtcaggta tgctgaggga gacactgttc tcaaactcca
    11281 ggccagggtg gtttccggag ggtccaccgg tggtctcatc tcttctctct caaaccatgg
    11341 atggtgcctt ttaaagaaaa tgacaccggc caggtgcggt ggctcatggc tgtaatccca
    11401 gcactttggg aggccgaggc gggtggatca cctgaggtcg gaagttcaac accagcctgg
    11461 ccaacatggt gaaaccccgt ctctattaaa aatacaaaaa ttaggccggg cgcggtggct
    11521 cacgcctgta atcccagcac tttgggaggc cgaggcgggc ggatcacgag gtcaggagat
    11581 cgagaccatc ccggctaaaa cggtgaaacc ccgtctctac taaaaataca aaaaattagc
    11641 cgggcgtagt ggcgggcgcc tgtagtccca gctacttggg aggctgaggc aggagaatgg
    11701 cgtgaacccg ggaggcggag cttgcagtga gccgagatcc cgccactgca ctccagcctg
    11761 ggcgacagag cgagactccg tctcaaaaaa aaaaaaaaaa aaaaaaaaat acaaaaatta
    11821 gctgggtgtg gtggtgggca cctgtaatcc cagctactga ggaggctgag gcatgagatt
    11881 cgcttgagcc tgggaggcag aggttgcagt gagctgagat cgcgccattg cactccagcc
    11941 tgggcaacag agtgagactc tgtctcaaaa aacaaacaaa aatcaaagtg ccaacctctg
    12001 ggctgtcccc tcctggcctg ccttgttcct gagctcacca gggtccttca ctctgtggca
    12061 ctggcctcag ccagcaccaa ggctgaaaga gcccattcag aggccagaaa gaaactgtta
    12121 ggctctgaag ccgtgggcca ggccaagaca gagattctga ggcatggttc tggaatgggt
    12181 ttttccgccc acaggcactc aaggaggaca gcattgggag atggctgggt ggagagtctg
    12241 cgggaggcta caaggcaaaa atcccctact ccccctgggc tccttcacag tttgtgtgtc
    12301 ctcacagagc aaggaagttc tgtgagcctc attcaatcat ctgaggttct tcccaggaaa
    12361 attatagtgg gaactctggc tttgatgaaa ctgctttttt ttcaaagcag tttgcaaaat
    12421 aaccggggcc ttaaaaaaca atctggatga aatggttttt gccatttcaa ttggttctct
    12481 tccaacgtgt cttccttcca gtaggtttcc agaatatttt ggtttcaaaa tttgggggca
    12541 cagatttgca aagtggattt caacatgtcg ggtttcaaag ggggctggct ttgaaagtgg
    12601 tctgggcttc taaaaggttt gattgaaaac tggtttggtt ctcagacatt ccagaagctt
    12661 ctcccggagt ccagagcctc ccagtggtgc tttgggaagg gggccaggca gggcgtcctg
    12721 gtgagtggac gtagggtggg cagggccacc cttgtcctgg gatgttgtgg aggacttggc
    12781 acgccctgtc tcaggcctgg ctctggctct gggtggatgt tttgcatctt cagaaaaaag
    12841 ctcctgtggg atatttctcc tccagccgac cccgcccccc agggcattcg agaaaagggt
    12901 gcttgtctct tggtgagagg gccacggttc cttcagtttc tccgttccta cgggcctctt
    12961 tctttttcct ttttgagacg gaatctcact gtgtcgccca ggctcactgc aacctatgcc
    13021 tcccgggttc aagtggttct catgcttcag cctcctgagt agctgggatt acaggtgtgt
    13081 gccaccacat ccagctaatt ttttgtattt ttagtagaga tggggttttc ccatgttggc
    13141 caggccggtc tcgaactcct gacctcgtga tctgcccacg ttggcctcca aagtgctagg
    13201 attataagtg tgagccacca caccaggccc gtttcatccc tgcctcagcc tgcatgccaa
    13261 gctccctggg tgcccgccca caggtgtcac cctttaagga gaaagtggcc tggggctgga
    13321 aacaggactt tgtagggaag gacagcgcgt gtgagaaaag gcctgttgat gggcacgcca
    13381 gggtttaatg gacggctctt tgaagtcatg ggctgagcca gaaggaattg tcatgatgtc
    13441 agtctcattt ccagatgggg aaagggagac ccaggctgcg gtgacccact gaggcacagg
    13501 atcagtgaga acccagccct ggctttgccc agacctctca agggcacttc gtgggaccat
    13561 tgccggccgt tgtggcatct gtccctggcc ttgcccccat ccgtctctgc acagcccagg
    13621 tgggtccgag ccagccccag gtctccaggg tcccagccca gcctctagaa gagccagcag
    13681 cagggctgga agggagagca catgctgggc cttggggctg ccttggacct caggccgttg
    13741 acaaggaaaa gcggagagtt gggaaaaaag gacaaatagc tgagcgccag ccgcagtctg
    13801 ggggacggga gcctcgtgcg actctcggag tgctggccag attggcagag gggaggagag
    13861 aggaaaaacc caggctctga ggaaaaacct ctccagccca gcgcgtggcc tggcattaac
    13921 ccactgcagc cccaggaagt ggttccaccc caggcagcca acaaggcggt gcctgaacta
    13981 tgtctgtgga tgacggagcc acgacctgtc cctcagggct cagcgctgcg gctatgtcca
    14041 gggacccctg gctgtgccca cgtcggggct tcccgttcct tacctgccat gagaatgaga
    14101 tgtccaggga cctccctgct ccagctggga cctgtggggc tttgtctgtt gtcccgagca
    14161 ccttggagat gtcatttcta acattttaac ccaggaatcc ccccaccaag ggacagagtg
    14221 caaggacatg atcgaacaga aaaagttctg gtacaagatc aaccccgtgg ggtggtgagt
    14281 gctgcagccc cgggcctcac atcctgccgt ccctgtggga gattggagcg gtcccagtgc
    14341 ccaccgctga ttctctggct ccagcaaccc ctccaggtgg atccgtccca cgcagcctgg
    14401 cctgaaacac tgcccagcca ctgggtccag taagacagag cctcgagtca ttctgccaag
    14461 aggatccaga aacacagact ttttctgggg tcctggaggc ttctggccca tggggagccc
    14521 ctgggtccca gcgatccagc cctgatgtgc tgagggtgca gggcccagct gcagagcaga
    14581 ggagagtggc ccccagggac cagcagcacg aaaggcacac tgaggcacac tggcaggcct
    14641 gggctgcaga gagcctgaag gtcatggggt agctggtgga agcaggaaga ccccatacag
    14701 cagcgaccac tgaggctggt gctgcacttt ctcagggaat tgagtgtggg ctcccaccat
    14761 cccggccact ggcttcctcc aaagcctcct cctcttacat cagcaaacct tctgttcggt
    14821 gaccccctca gtgaccctct gtgcttgcct tcgtggtctt cctcatggag gatttcgggt
    14881 cagcgtgggg gtcagaggtc atttcccata ccccctcaaa ggtacttctt gctgtggtcc
    14941 ccacactctg acaccctctt ctgaaatgaa cacttttttg ttgttgttgt tgagacagag
    15001 tcttgctctg tcgcccaggc tggagtgcag tggcacaatc ttagctcact gcaacctcca
    15061 cctcccaggt tcaagcgatt ttcatgcctc agcttcccga gtagctggga ttacaggcat
    15121 gtgccaccaa gcccagctaa tttttgtatt tttagtagag atgaggtttc accatgttgg
    15181 ccaggctggt ctcgaacttc tgacctcaag tgattcgccc gcctcggcct cccaaagtgc
    15241 tgggattaca agcatgagcc actgcgcccg gccacaaaca cttttttttt tttaaacaag
    15301 tgaaaacaag tttattaaca aagttaagaa ataaagaatg gctattccat agggagagca
    15361 gcctgaataa acactttttt tgttgttgtt gaggcagagc ctcactctgt cgcccaggca
    15421 ggagtgcagt ggtgcgacct cagctctcag ctccctgcac cgtccacctc ctgggttcaa
    15481 gcgcttcttc cccaagtagc tgggactata ggcacacacc accatgcccg gctaattttt
    15541 ctctttttag tagagacagg atttcgccat gtgggtcggg ctggtctcga actcctggtc
    15601 tcaagtgatc cgccctcctc ggccttccaa aatgctagga ttacaggcat gagccgccgt
    15661 gcctggccac aaacatattt taaatttgaa caataaagga actggaccag gaagtgtgga
    15721 gttggggaga ccctgctctc gccaggtgcc ttcccggcag gttgtctctg cctccccttg
    15781 caccccttga atggcgggct ccctctccca tagcccctgc caggctggat aaccaacttc
    15841 attcttatac ccgtccgaat ctgccccctg ttaatggcac ccaccagtcc ttgtccaaac
    15901 ctcgaagctc aggacctcac agagcaccac gtcctcttct gtgtcatatg cagctggatc
    15961 tcagtaaatg gcaatgtctt ctcta
//
