LOCUS       NM_003654               3906 bp    mRNA    linear   PRI 06-JUN-2024
DEFINITION  Homo sapiens carbohydrate sulfotransferase 1 (CHST1), mRNA.
ACCESSION   NM_003654
VERSION     NM_003654.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3906)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   2  (bases 1 to 3906)
  AUTHORS   Fujiwara,M., Kobayashi,M., Hoshino,H., Uchimura,K., Nakada,T.,
            Masumoto,J., Sakai,Y., Fukuda,M. and Nakayama,J.
  TITLE     Expression of long-form N-acetylglucosamine-6-O-sulfotransferase 1
            in human high endothelial venules
  JOURNAL   J Histochem Cytochem 60 (5), 397-407 (2012)
   PUBMED   22260995
  REMARK    GeneRIF: found endogenous expression of the long form of the enzyme
            in human tissue, predominantly in the trans-Golgi network of
            endothelial cells that form human high endothelial venules.
REFERENCE   3  (bases 1 to 3906)
  AUTHORS   Seko,A., Ohkura,T., Ideo,H. and Yamashita,K.
  TITLE     Novel O-linked glycans containing 6'-sulfo-Gal/GalNAc of MUC1
            secreted from human breast cancer YMB-S cells: possible
            carbohydrate epitopes of KL-6(MUC1) monoclonal antibody
  JOURNAL   Glycobiology 22 (2), 181-195 (2012)
   PUBMED   21880669
  REMARK    GeneRIF: Epitope expressing changes with suppression or
            over-expression of the Gal6ST (Gal 6-O-sulfotransferase) gene,
            suggesting that Gal6ST is involved in the biosynthesis of the
            unique epitopes of KL-6/mAb.
REFERENCE   4  (bases 1 to 3906)
  AUTHORS   van Roij,M.H., Mizumoto,S., Yamada,S., Morgan,T.,
            Tan-Sindhunata,M.B., Meijers-Heijboer,H., Verbeke,J.I., Markie,D.,
            Sugahara,K. and Robertson,S.P.
  TITLE     Spondyloepiphyseal dysplasia, Omani type: further definition of the
            phenotype
  JOURNAL   Am J Med Genet A 146A (18), 2376-2384 (2008)
   PUBMED   18698629
  REMARK    GeneRIF: A new point mutation in C6ST-1 is associated with
            spondyloepiphyseal dysplasia, Omani type.
REFERENCE   5  (bases 1 to 3906)
  AUTHORS   Kitayama,K., Hayashida,Y., Nishida,K. and Akama,T.O.
  TITLE     Enzymes responsible for synthesis of corneal keratan sulfate
            glycosaminoglycans
  JOURNAL   J Biol Chem 282 (41), 30085-30096 (2007)
   PUBMED   17690104
  REMARK    GeneRIF: sulfated keratan sulfate is produced by beta3GNT7,
            beta4GalT4, CGn6ST, and KSG6ST
REFERENCE   6  (bases 1 to 3906)
  AUTHORS   Bistrup,A., Bhakta,S., Lee,J.K., Belov,Y.Y., Gunn,M.D., Zuo,F.R.,
            Huang,C.C., Kannagi,R., Rosen,S.D. and Hemmerich,S.
  TITLE     Sulfotransferases of two specificities function in the
            reconstitution of high endothelial cell ligands for L-selectin
  JOURNAL   J Cell Biol 145 (4), 899-910 (1999)
   PUBMED   10330415
REFERENCE   7  (bases 1 to 3906)
  AUTHORS   Li,X. and Tedder,T.F.
  TITLE     CHST1 and CHST2 sulfotransferases expressed by human vascular
            endothelial cells: cDNA cloning, expression, and chromosomal
            localization
  JOURNAL   Genomics 55 (3), 345-347 (1999)
   PUBMED   10049591
REFERENCE   8  (bases 1 to 3906)
  AUTHORS   Mazany,K.D., Peng,T., Watson,C.E., Tabas,I. and Williams,K.J.
  TITLE     Human chondroitin 6-sulfotransferase: cloning, gene structure, and
            chromosomal localization
  JOURNAL   Biochim Biophys Acta 1407 (1), 92-97 (1998)
   PUBMED   9639683
REFERENCE   9  (bases 1 to 3906)
  AUTHORS   Fukuta,M., Inazawa,J., Torii,T., Tsuzuki,K., Shimada,E. and
            Habuchi,O.
  TITLE     Molecular cloning and characterization of human keratan sulfate
            Gal-6-sulfotransferase
  JOURNAL   J Biol Chem 272 (51), 32321-32328 (1997)
   PUBMED   9405439
REFERENCE   10 (bases 1 to 3906)
  AUTHORS   Williams,K.J.
  TITLE     Atherosclerosis: cell biology and lipoproteins
  JOURNAL   Curr Opin Lipidol 7 (6), U202-U208 (1996)
   PUBMED   9117134
  REMARK    Review article
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DB493321.1, AC087442.13,
            DA193787.1, BC022567.1, BI917296.1 and AI793094.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_003654.5.
            
            Summary: This locus encodes a member of the keratin
            sulfotransferase family of proteins. The encoded enzyme catalyzes
            the sulfation of the proteoglycan keratin. [provided by RefSeq, Aug
            2011].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            transcript and genomic sequence to make the sequence consistent
            with the reference genome assembly. The extent of this RefSeq
            transcript is supported by transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC022567.1, AB003791.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2144120 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000308064.7/ ENSP00000309270.2
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-6                 DB493321.1         35-40
            7-28                AC087442.13        90786-90807         c
            29-171              DA193787.1         1-143
            172-650             BC022567.1         172-650
            651-883             BI917296.1         318-550
            884-884             AC087442.13        75902-75902         c
            885-2138            BC022567.1         885-2138
            2139-2144           AC087442.13        74642-74647         c
            2145-2718           BC022567.1         2144-2717
            2719-3367           AC087442.13        73419-74067         c
            3368-3906           AI793094.1         8-546               c
FEATURES             Location/Qualifiers
     source          1..3906
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11p11.2"
     gene            1..3906
                     /gene="CHST1"
                     /gene_synonym="C6ST; GST-1; KS6ST; KSGal6ST; KSST"
                     /note="carbohydrate sulfotransferase 1"
                     /db_xref="GeneID:8534"
                     /db_xref="HGNC:HGNC:1969"
                     /db_xref="MIM:603797"
     exon            1..445
                     /gene="CHST1"
                     /gene_synonym="C6ST; GST-1; KS6ST; KSGal6ST; KSST"
                     /inference="alignment:Splign:2.1.0"
     exon            446..531
                     /gene="CHST1"
                     /gene_synonym="C6ST; GST-1; KS6ST; KSGal6ST; KSST"
                     /inference="alignment:Splign:2.1.0"
     exon            532..629
                     /gene="CHST1"
                     /gene_synonym="C6ST; GST-1; KS6ST; KSGal6ST; KSST"
                     /inference="alignment:Splign:2.1.0"
     exon            630..3906
                     /gene="CHST1"
                     /gene_synonym="C6ST; GST-1; KS6ST; KSGal6ST; KSST"
                     /inference="alignment:Splign:2.1.0"
     CDS             672..1907
                     /gene="CHST1"
                     /gene_synonym="C6ST; GST-1; KS6ST; KSGal6ST; KSST"
                     /EC_number="2.8.2.21"
                     /note="carbohydrate (chondroitin 6/keratan)
                     sulfotransferase 1;
                     galactose/N-acetylglucosamine/N-acetylglucosamine
                     6-O-sulfotransferase 1; carbohydrate (keratan sulfate
                     Gal-6) sulfotransferase 1; Keratan sulfotransferase"
                     /codon_start=1
                     /product="carbohydrate sulfotransferase 1"
                     /protein_id="NP_003645.1"
                     /db_xref="CCDS:CCDS7913.1"
                     /db_xref="GeneID:8534"
                     /db_xref="HGNC:HGNC:1969"
                     /db_xref="MIM:603797"
                     /translation="MQCSWKAVLLLALASIAIQYTAIRTFTAKSFHTCPGLAEAGLAE
                     RLCEESPTFAYNLSRKTHILILATTRSGSSFVGQLFNQHLDVFYLFEPLYHVQNTLIP
                     RFTQGKSPADRRVMLGASRDLLRSLYDCDLYFLENYIKPPPVNHTTDRIFRRGASRVL
                     CSRPVCDPPGPADLVLEEGDCVRKCGLLNLTVAAEACRERSHVAIKTVRVPEVNDLRA
                     LVEDPRLNLKVIQLVRDPRGILASRSETFRDTYRLWRLWYGTGRKPYNLDVTQLTTVC
                     EDFSNSVSTGLMRPPWLKGKYMLVRYEDLARNPMKKTEEIYGFLGIPLDSHVARWIQN
                     NTRGDPTLGKHKYGTVRNSAATAEKWRFRLSYDIVAFAQNACQQVLAQLGYKIAASEE
                     ELKNPSVSLVEERDFRPFS"
     regulatory      2699..2704
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CHST1"
                     /gene_synonym="C6ST; GST-1; KS6ST; KSGal6ST; KSST"
                     /note="hexamer: AATAAA"
     polyA_site      2716
                     /gene="CHST1"
                     /gene_synonym="C6ST; GST-1; KS6ST; KSGal6ST; KSST"
                     /note="major polyA site"
     regulatory      3882..3887
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CHST1"
                     /gene_synonym="C6ST; GST-1; KS6ST; KSGal6ST; KSST"
                     /note="hexamer: AATACA"
     polyA_site      3906
                     /gene="CHST1"
                     /gene_synonym="C6ST; GST-1; KS6ST; KSGal6ST; KSST"
ORIGIN      
        1 gctcggagct gtagccgccg ctgccgccgc tgctcttggc tcggggacgc gggcgggcgc
       61 tccgagaggc tctggtgcct gcggctgctg ctctcctccg gcgcccgggg ctgcggcgct
      121 ccgggtcccg gcccctgtcc cggcatctct cgggtccctg agccccggcc ccgggtcccg
      181 cgtccggccg gagagaacgc actcagctgc tcgagccgcc ccgggctcgc gcccccgggc
      241 catgaagagg ggcccgtgag gcgcgcgcgg agtcggagga agaggaggga ggagggaggc
      301 cgaggaggag ggcgcgggag gcggaggatg ccgccgcggc tgctgccgcc gccgccaccc
      361 gcgggtcccc ggcgaccctg actccagacc cgaggatgga gccggcgctg ggcgctgcag
      421 ctgctcccgg cgcgtccccg accaggtagc tggtgtcact tcggtgtggt tggaagaaga
      481 ctttctcccc agctgcattc ccggaggcgc cctttcgacc tggaggccgg gtctgctggc
      541 cacagggctg ccgcactggc tgggactgcc agctgggcct ggagacgctg gtggctgtgg
      601 actccccagc ttggagcagt ccctctttga cctcacccct tggagaagca gccccatgaa
      661 ggtgcccagc catgcaatgt tcctggaagg ccgtcctcct ccttgccctg gcctccattg
      721 ccatccagta cacggccatc cgcaccttca ccgccaagtc ctttcacacc tgccccgggc
      781 tggcagaggc cgggctggcc gagcgactgt gcgaggagag ccccaccttc gcctacaacc
      841 tctcccgcaa gacccacatc ctcatcctgg ccaccacgcg cagtggctcc tccttcgtgg
      901 gccagctctt caaccagcac ctggacgtct tctacctgtt tgagcccctc taccacgtcc
      961 agaacacgct catcccccgc ttcacccagg gcaagagccc ggccgaccgg cgggtcatgc
     1021 taggcgccag ccgcgacctc ctgcggagcc tctacgactg cgacctctac ttcctggaga
     1081 actacatcaa gccgccgccg gtcaaccaca ccaccgacag gatcttccgc cgcggggcca
     1141 gccgggtcct ctgctcccgg cctgtgtgcg accctccggg gccagccgac ctggtcctgg
     1201 aggaggggga ctgtgtgcgc aagtgcgggc tactcaacct gaccgtggcg gccgaggcgt
     1261 gccgcgagcg cagccacgtg gccatcaaga cggtgcgcgt gcccgaggtg aacgacctgc
     1321 gcgccctggt ggaagacccg cgattaaacc tcaaggtcat ccagctggtc cgagaccccc
     1381 gcggcattct ggcttcgcgc agcgagacct tccgcgacac gtaccggctc tggcggctct
     1441 ggtacggcac cgggaggaaa ccctacaacc tggacgtgac gcagctgacc acggtgtgcg
     1501 aggacttctc caactccgtg tccaccggcc tcatgcggcc cccgtggctc aagggcaagt
     1561 acatgttggt gcgctacgag gacctggctc ggaaccctat gaagaagacc gaggagatct
     1621 acgggttcct gggcatcccg ctggacagcc acgtggcccg ctggatccag aacaacacgc
     1681 ggggcgaccc caccctgggc aagcacaaat acggcaccgt gcgaaactcg gcggccacgg
     1741 ccgagaagtg gcgcttccgc ctctcctacg acatcgtggc ctttgcccag aacgcctgcc
     1801 agcaggtgct ggcccagctg ggctacaaga tcgccgcctc ggaggaggag ctgaagaacc
     1861 cctcggtcag cctggtggag gagcgggact tccgcccctt ctcgtgaccc gggcggtgcg
     1921 ggtgggggcg ggaggcgcaa ggtgtcggtt ttgataaaat ggaccgtttt taactgttgc
     1981 cttattaacc cctccctctc ccacctcatc ttcgtgtcct tcctgccccc agctcacccc
     2041 actcccttct gccccttttt tgtctctgaa atttgcacta cgtcttggac gggaatcact
     2101 ggggcagagg gcgcctgaag tagggtcccg cccccccccc ccccattcag acacatggat
     2161 gttgggtctc tgtgcggacg gtgacaatgt ttacaagcac cacatttaca catccacaca
     2221 cgcacacggg cactcgcgag gcgacttctc aagcttttga atgggtgagt ggtcgggtat
     2281 ctagtttttg cactgtctta ctattcaagg taagaggata caaacaagag gaccacttgt
     2341 ctctaattta tgaatggtgt ccatcctttc cccatccctg cctcctgccc ctgacgccca
     2401 tttcccccct tagagcagcg aaactgcccc ctcctgcccg cccttgcctg tcggtgaggc
     2461 aggtttttac tgtgaggtga acgtggacct gtttctgttt ccagtctgtg gtgatgctgt
     2521 ctgtctgtct gagtctcgtg gccgcccctg gaccagtgat gactgatgaa tcttatgagc
     2581 ttctgattga tctcggggtc catctgtgat atttctttgt gccaaaaaga aaaaaaaaga
     2641 gtggatcagt ttgctaaatg aacattgaaa ttgaaatgct ttatctgtgt tttctgtaaa
     2701 taaaagagtg caataatctc tgtgtgtgat tgcaggacat tctgaatggg tacgagaggg
     2761 cctcagccgg gtctgggtgt tcctagcttt ggggaggaca gcgagagaga ggtggaggtg
     2821 ggaattaaat gacaagtctg cctttcagaa ctcttgtcac cctcaacact gagtttagtt
     2881 taggtttttg ttttgttttg ttttgagaca gagtctcgct ctgtcaccca ggctggagtg
     2941 caatggcgcc atctcggctc actgcaacct ctgcctccca ggttcaagca gttctcccgc
     3001 ctcagcctcc cgagtgactg ggactacagg tgcacactgc cacacctggc tatttttttt
     3061 tttttttgga ttttagtaga gacggggttt caccgtgttg tccaggctgg tcgcgaactc
     3121 ctgagctctg ggcattcctc ctgcctcagc ctcccaaagt gctgggattg cagacttgag
     3181 ccacctcacc cagccgtttt ttttttcctt cagtcagttg agaccgggta acttatagcc
     3241 agggataatg ttcaaagtct ttaacaagtg gtatggcttg ctgaatcaga gtagctggag
     3301 gtaagaatga catcctggaa tcccttcttg tgctaaatgt tacccattta gttggtgggt
     3361 ctgggtctag gatagccagg gagggggaaa cttcaagggg tggggtgcct cagggcagtg
     3421 gctgctgacc cacagacctg gaagtatttg actatttgaa taactggtac aggtttactg
     3481 gtgtctacac tggcagagaa tctgctgtgc ttcctcccct tcccttcgtg gcaatctagc
     3541 aggttgctcc aggcagcttc cccagctctt attccaagaa aacctgcccc tggggtggct
     3601 gcaggtcaca gctttgttgt cctgggacaa aggagtgtct ggtgattagg cagccccagg
     3661 ggtcctagcc aactggaaga agggcttcct gaggaaacac agtcctgggc tattttatat
     3721 gtgtcaggag gatgtagaca gttcaacagc tctagcaaat gccttaacta atagtggctt
     3781 aatcaagata ggtgattttc ttttgtaaaa aattttactg gggtaaaata acagaatgta
     3841 aaatttacca tcttaacctt tttaaggtgt accattcatt aaatacattc acaacgtgtg
     3901 ccacca
//
