LOCUS       NM_017581               2272 bp    mRNA    linear   PRI 01-JUN-2024
DEFINITION  Homo sapiens cholinergic receptor nicotinic alpha 9 subunit
            (CHRNA9), mRNA.
ACCESSION   NM_017581
VERSION     NM_017581.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Gu,S., Knowland,D., Matta,J.A., O'Carroll,M.L., Davini,W.B.,
            Dhara,M., Kweon,H.J. and Bredt,D.S.
  TITLE     Hair cell alpha9alpha10 nicotinic acetylcholine receptor functional
            expression regulated by ligand binding and deafness gene products
  JOURNAL   Proc Natl Acad Sci U S A 117 (39), 24534-24544 (2020)
   PUBMED   32929005
  REMARK    GeneRIF: Hair cell alpha9alpha10 nicotinic acetylcholine receptor
            functional expression regulated by ligand binding and deafness gene
            products.
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Hone,A.J., Servent,D. and McIntosh,J.M.
  TITLE     alpha9-containing nicotinic acetylcholine receptors and the
            modulation of pain
  JOURNAL   Br J Pharmacol 175 (11), 1915-1927 (2018)
   PUBMED   28662295
  REMARK    GeneRIF: REVIEW: details the history and state of the field
            regarding the role that alpha9-containing nAChRs may play in
            neuropathic pain
            Review article
REFERENCE   3  (bases 1 to 2272)
  AUTHORS   Mohammadi,S.A., Burton,T.J. and Christie,M.J.
  TITLE     alpha9-nAChR knockout mice exhibit dysregulation of stress
            responses, affect and reward-related behaviour
  JOURNAL   Behav Brain Res 328, 105-114 (2017)
   PUBMED   28408300
  REMARK    GeneRIF: Behavioural and physiological stress-induced changes
            observed in alpha9-nAChR KO mice demonstrate a novel role of the
            alpha9alpha10-nAChR in mounting a normal stress response. The lack
            of functioning alpha9alpha10-nAChRs leads to negative affective
            changes after a relatively short period of stress. KO mice
            exhibited unusual patterns of circadian activity and a dysfunction
            in reward seeking after a period of reduced acc...
REFERENCE   4  (bases 1 to 2272)
  AUTHORS   Romero,H.K., Christensen,S.B., Di Cesare Mannelli,L., Gajewiak,J.,
            Ramachandra,R., Elmslie,K.S., Vetter,D.E., Ghelardini,C.,
            Iadonato,S.P., Mercado,J.L., Olivera,B.M. and McIntosh,J.M.
  TITLE     Inhibition of alpha9alpha10 nicotinic acetylcholine receptors
            prevents chemotherapy-induced neuropathic pain
  JOURNAL   Proc Natl Acad Sci U S A 114 (10), E1825-E1832 (2017)
   PUBMED   28223528
  REMARK    GeneRIF: Chemotherapy induced cold allodynia is reduced in alpha 9
            knockout mice.
REFERENCE   5  (bases 1 to 2272)
  AUTHORS   Richter,K., Mathes,V., Fronius,M., Althaus,M., Hecker,A.,
            Krasteva-Christ,G., Padberg,W., Hone,A.J., McIntosh,J.M.,
            Zakrzewicz,A. and Grau,V.
  TITLE     Phosphocholine - an agonist of metabotropic but not of ionotropic
            functions of alpha9-containing nicotinic acetylcholine receptors
  JOURNAL   Sci Rep 6, 28660 (2016)
   PUBMED   27349288
  REMARK    GeneRIF: phosphocholine inhibits ion-channel function of ATP
            receptor P2X7 in monocytic cells via nAChR containing alpha9 and
            alpha10 subunits.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2272)
  AUTHORS   Valor,L.M., Castillo,M., Ortiz,J.A. and Criado,M.
  TITLE     Transcriptional regulation by activation and repression elements
            located at the 5'-noncoding region of the human alpha9 nicotinic
            receptor subunit gene
  JOURNAL   J Biol Chem 278 (39), 37249-37255 (2003)
   PUBMED   12860975
  REMARK    GeneRIF: two cis-elements of the alpha9 nicotinic receptor subunit
            promoter control in opposite ways the basal transcriptional
            activity of the gene
REFERENCE   7  (bases 1 to 2272)
  AUTHORS   Sgard,F., Charpantier,E., Bertrand,S., Walker,N., Caput,D.,
            Graham,D., Bertrand,D. and Besnard,F.
  TITLE     A novel human nicotinic receptor subunit, alpha10, that confers
            functionality to the alpha9-subunit
  JOURNAL   Mol Pharmacol 61 (1), 150-159 (2002)
   PUBMED   11752216
REFERENCE   8  (bases 1 to 2272)
  AUTHORS   Lustig,L.R. and Peng,H.
  TITLE     Chromosome location and characterization of the human nicotinic
            acetylcholine receptor subunit alpha (alpha) 9 (CHRNA9) gene
  JOURNAL   Cytogenet Genome Res 98 (2-3), 154-159 (2002)
   PUBMED   12697997
  REMARK    GeneRIF: Chromosome location and characterization of the CHRNA9
            gene.
REFERENCE   9  (bases 1 to 2272)
  AUTHORS   Harrington,J.J., Sherf,B., Rundlett,S., Jackson,P.D., Perry,R.,
            Cain,S., Leventhal,C., Thornton,M., Ramachandran,R.,
            Whittington,J., Lerner,L., Costanzo,D., McElligott,K., Boozer,S.,
            Mays,R., Smith,E., Veloso,N., Klika,A., Hess,J., Cothren,K., Lo,K.,
            Offenbacher,J., Danzig,J. and Ducar,M.
  TITLE     Creation of genome-wide protein expression libraries using random
            activation of gene expression
  JOURNAL   Nat Biotechnol 19 (5), 440-445 (2001)
   PUBMED   11329013
REFERENCE   10 (bases 1 to 2272)
  AUTHORS   Nguyen,V.T., Ndoye,A. and Grando,S.A.
  TITLE     Novel human alpha9 acetylcholine receptor regulating keratinocyte
            adhesion is targeted by Pemphigus vulgaris autoimmunity
  JOURNAL   Am J Pathol 157 (4), 1377-1391 (2000)
   PUBMED   11021840
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BP870578.1, BC113549.1,
            AF227732.1, AJ243342.1, AC118275.4 and BG218331.1.
            
            On Nov 24, 2018 this sequence version replaced NM_017581.3.
            
            Summary: This gene is a member of the ligand-gated ionic channel
            family and nicotinic acetylcholine receptor gene superfamily. It
            encodes a plasma membrane protein that forms homo- or
            hetero-oligomeric divalent cation channels. This protein is
            involved in cochlea hair cell development and is also expressed in
            the outer hair cells (OHCs) of the adult cochlea. [provided by
            RefSeq, Feb 2012].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AJ243342.1, BC113549.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1968540,
                                           SAMEA2142853 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000310169.3/ ENSP00000312663.2
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-40                BP870578.1         6-45
            41-1459             BC113549.1         1-1419
            1460-1563           AF227732.1         1307-1410
            1564-1751           AJ243342.1         1445-1632
            1752-1752           AC118275.4         58680-58680
            1753-2011           AJ243342.1         1634-1892
            2012-2272           BG218331.1         17-277              c
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="4"
                     /map="4p14"
     gene            1..2272
                     /gene="CHRNA9"
                     /gene_synonym="HSA243342; NACHRA9"
                     /note="cholinergic receptor nicotinic alpha 9 subunit"
                     /db_xref="GeneID:55584"
                     /db_xref="HGNC:HGNC:14079"
                     /db_xref="MIM:605116"
     exon            1..199
                     /gene="CHRNA9"
                     /gene_synonym="HSA243342; NACHRA9"
                     /inference="alignment:Splign:2.1.0"
     CDS             136..1575
                     /gene="CHRNA9"
                     /gene_synonym="HSA243342; NACHRA9"
                     /note="cholinergic receptor, nicotinic, alpha polypeptide
                     9; acetylcholine receptor, neuronal nicotinic, alpha-9
                     subunit; neuronal acetylcholine receptor protein, alpha-9
                     subunit; NACHR alpha 9; nicotinic acetylcholine receptor
                     subunit alpha 9; cholinergic receptor, nicotinic, alpha 9
                     (neuronal); cholinergic receptor, nicotinic alpha 9"
                     /codon_start=1
                     /product="neuronal acetylcholine receptor subunit alpha-9
                     precursor"
                     /protein_id="NP_060051.2"
                     /db_xref="CCDS:CCDS3459.1"
                     /db_xref="GeneID:55584"
                     /db_xref="HGNC:HGNC:14079"
                     /db_xref="MIM:605116"
                     /translation="MNWSHSCISFCWIYFAASRLRAAETADGKYAQKLFNDLFEDYSN
                     ALRPVEDTDKVLNVTLQITLSQIKDMDERNQILTAYLWIRQIWHDAYLTWDRDQYDGL
                     DSIRIPSDLVWRPDIVLYNKADDESSEPVNTNVVLRYDGLITWDAPAITKSSCVVDVT
                     YFPFDNQQCNLTFGSWTYNGNQVDIFNALDSGDLSDFIEDVEWEVHGMPAVKNVISYG
                     CCSEPYPDVTFTLLLKRRSSFYIVNLLIPCVLISFLAPLSFYLPAASGEKVSLGVTIL
                     LAMTVFQLMVAEIMPASENVPLIGKYYIATMALITASTALTIMVMNIHFCGAEARPVP
                     HWARVVILKYMSRVLFVYDVGESCLSPHHSRERDHLTKVYSKLPESNLKAARNKDLSR
                     KKDMNKRLKNDLGCQGKNPQEAESYCAQYKVLTRNIEYIAKCLKDHKATNSKGSEWKK
                     VAKVIDRFFMWIFFIMVFVMTILIIARAD"
     exon            200..345
                     /gene="CHRNA9"
                     /gene_synonym="HSA243342; NACHRA9"
                     /inference="alignment:Splign:2.1.0"
     exon            346..500
                     /gene="CHRNA9"
                     /gene_synonym="HSA243342; NACHRA9"
                     /inference="alignment:Splign:2.1.0"
     exon            501..1033
                     /gene="CHRNA9"
                     /gene_synonym="HSA243342; NACHRA9"
                     /inference="alignment:Splign:2.1.0"
     exon            1034..2272
                     /gene="CHRNA9"
                     /gene_synonym="HSA243342; NACHRA9"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2208..2213
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CHRNA9"
                     /gene_synonym="HSA243342; NACHRA9"
                     /note="hexamer: ATTAAA"
     polyA_site      2227
                     /gene="CHRNA9"
                     /gene_synonym="HSA243342; NACHRA9"
     regulatory      2256..2261
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CHRNA9"
                     /gene_synonym="HSA243342; NACHRA9"
                     /note="hexamer: AATAAG"
     polyA_site      2272
                     /gene="CHRNA9"
                     /gene_synonym="HSA243342; NACHRA9"
                     /note="major polyA site"
ORIGIN      
        1 aatgcaagcc tgagctctcc cgccataagg ctgcagcggt gtgggctcct tgtgcccaga
       61 tcctttgtat tcataggggg aagtggaaga ccacgctgcc tgactgagac tttattatag
      121 aggctcagga aaaagatgaa ctggtcccat tcctgcatct ccttttgctg gatctacttt
      181 gctgcttcca gactgagagc tgcagagacg gcagatggaa aatatgctca gaagttgttt
      241 aatgaccttt ttgaagatta ttctaatgct cttcgtccag tggaagatac agataaagtc
      301 ctgaatgtga ccctgcagat tacgctctct cagattaagg atatggatga aagaaaccaa
      361 attctgactg cttatttgtg gatccgccaa atctggcacg atgcctatct cacgtgggac
      421 cgagatcagt acgatggcct agactccatc aggatcccca gtgacctcgt gtggaggcca
      481 gacatcgtct tatataacaa ggctgatgat gaatcttcag agcctgtgaa caccaatgtg
      541 gtcctgcggt atgatgggct gatcacctgg gatgcaccgg ccatcaccaa aagctcctgt
      601 gtggtggatg tcacctactt cccttttgac aaccagcagt gcaacctgac ttttggttcc
      661 tggacctaca atggcaatca ggtggacata ttcaacgcct tggacagcgg agatctctct
      721 gacttcattg aagatgtgga atgggaggtc catggcatgc ccgctgtgaa gaatgtgatc
      781 tcctatggct gctgctctga gccttacccg gatgtcacat tcaccctcct tctgaagagg
      841 aggtcctcgt tctatatcgt caacctcctc atcccatgcg tcctcatatc ttttctggct
      901 cctctgagtt tttatctccc agcagcctcc ggagaaaagg tctccctggg agtgaccatc
      961 ctgttggcca tgactgtatt tcagctaatg gtggcagaaa tcatgccggc ctcagaaaat
     1021 gtgcccctga taggtaaata ctacatagcc acgatggccc tgatcacagc ctccactgcg
     1081 ttgaccatca tggtgatgaa tatccacttc tgtggggccg aggcccggcc ggtgccacac
     1141 tgggccaggg tggtcatcct gaaatacatg tccagggtct tgtttgtcta tgatgtgggt
     1201 gaaagctgcc tcagcccgca ccacagtaga gagcgggacc acctcacgaa agtttatagc
     1261 aaactcccag agtctaacct gaaagcagcc aggaacaaag acctttccag aaagaaggac
     1321 atgaacaaac gcttaaagaa cgacctgggc tgccagggta agaaccctca ggaggccgag
     1381 agttactgtg cacagtacaa agtgctgacg aggaatattg agtacatcgc caagtgcctc
     1441 aaagaccaca aggccaccaa ttccaagggg agtgaatgga agaaggtggc gaaagtcata
     1501 gaccgattct tcatgtggat ttttttcatt atggtgtttg tgatgactat tttgatcata
     1561 gcaagagcgg attagtcaca gatattggct ttgctatctg ggtagaaatt aatacaattc
     1621 cctgtgatct attccaatgt tcttccaaga tttttgttca tctataattt aggggttatg
     1681 ttgtctgtgc tttttatttt tagcttcaaa tgaatgtcga agctatctgc tctgttaaat
     1741 taagcagaag aaccaaaatt tcaagggtag gaagatggaa gaaataggga aagagccctt
     1801 ttatagccca ccgtagtggt gggtgactgg cctctagttt atataattat ttacctcttt
     1861 ggcagtacag ataacaaaat acactttact ggtaaaattt aaaacaaaaa aggcaaaaca
     1921 aaacaaactc attttcccct tagttcccat taaaatatgc atttgaaaaa tgaaatagtc
     1981 atatatatat tagtgtgatt taagtctcaa tggtacctta ttcaggcata gtgcatttga
     2041 agtcatatct caaatagtgg aataaaaggt tttgtcccat gtaaagaaag tctaagcatc
     2101 agctgtttaa tggggaaaag aactttcttc ataatggtga tgtcgtatat gttggttatg
     2161 ttttatataa acccaaaaga tgaagtcatt cctgcattta ccctttgatt aaaatatgat
     2221 agatgaagct gttcctgcat ttagtcttgt attaaaataa ggtgattagc aa
//
