LOCUS       NM_000746               6149 bp    mRNA    linear   PRI 20-FEB-2025
DEFINITION  Homo sapiens cholinergic receptor nicotinic alpha 7 subunit
            (CHRNA7), transcript variant 1, mRNA.
ACCESSION   NM_000746 XM_937723
VERSION     NM_000746.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6149)
  AUTHORS   Oz,M., Kury,L.A., Sadek,B. and Mahgoub,M.O.
  TITLE     The role of nicotinic acetylcholine receptors in the
            pathophysiology and pharmacotherapy of autism spectrum disorder:
            Focus on alpha7 nicotinic receptors
  JOURNAL   Int J Biochem Cell Biol 174, 106634 (2024)
   PUBMED   39094731
  REMARK    GeneRIF: The role of nicotinic acetylcholine receptors in the
            pathophysiology and pharmacotherapy of autism spectrum disorder:
            Focus on alpha7 nicotinic receptors.
            Review article
REFERENCE   2  (bases 1 to 6149)
  AUTHORS   Di,T., Chen,Y., Zhou,Z., Liu,J., Du,Y., Feng,C., Zhu,B. and Wang,L.
  TITLE     Effect of alpha7 nAChR-autophagy axis of deciduous tooth pulp stem
            cells in regulating IL-1beta in the process of physiological root
            resorption of deciduous teeth
  JOURNAL   J Mol Med (Berl) 102 (9), 1135-1149 (2024)
   PUBMED   39002004
  REMARK    GeneRIF: Effect of alpha7 nAChR-autophagy axis of deciduous tooth
            pulp stem cells in regulating IL-1beta in the process of
            physiological root resorption of deciduous teeth.
REFERENCE   3  (bases 1 to 6149)
  AUTHORS   O'Brien,B.C.V., Thao,S., Weber,L., Danielson,H.L., Boldt,A.D.,
            Hueffer,K. and Weltzin,M.M.
  TITLE     The human alpha7 nicotinic acetylcholine receptor is a host target
            for the rabies virus glycoprotein
  JOURNAL   Front Cell Infect Microbiol 14, 1394713 (2024)
   PUBMED   38836054
  REMARK    GeneRIF: The human alpha7 nicotinic acetylcholine receptor is a
            host target for the rabies virus glycoprotein.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 6149)
  AUTHORS   George,A.A., John,S.J., Lucero,L.M., Eaton,J.B., Jaiswal,E.,
            Christensen,S.B., Gajewiak,J., Watkins,M., Cao,Y., Olivera,B.M.,
            Im,W., McIntosh,J.M. and Whiteaker,P.
  TITLE     Analogs of alpha-conotoxin PnIC selectively inhibit alpha7beta2-
            over alpha7-only subtype nicotinic acetylcholine receptors via a
            novel allosteric mechanism
  JOURNAL   FASEB J 38 (1), e23374 (2024)
   PUBMED   38161283
REFERENCE   5  (bases 1 to 6149)
  AUTHORS   Sluchanko,N.N., Kapitonova,A.A., Shulepko,M.A., Kukushkin,I.D.,
            Kulbatskii,D.S., Tugaeva,K.V., Varfolomeeva,L.A., Minyaev,M.E.,
            Boyko,K.M., Popov,V.O., Kirpichnikov,M.P. and Lyukmanova,E.N.
  TITLE     Crystal structure reveals canonical recognition of the
            phosphorylated cytoplasmic loop of human alpha7 nicotinic
            acetylcholine receptor by 14-3-3 protein
  JOURNAL   Biochem Biophys Res Commun 682, 91-96 (2023)
   PUBMED   37804592
  REMARK    GeneRIF: Crystal structure reveals canonical recognition of the
            phosphorylated cytoplasmic loop of human alpha7 nicotinic
            acetylcholine receptor by 14-3-3 protein.
REFERENCE   6  (bases 1 to 6149)
  AUTHORS   Chavez-Noriega,L.E., Crona,J.H., Washburn,M.S., Urrutia,A.,
            Elliott,K.J. and Johnson,E.C.
  TITLE     Pharmacological characterization of recombinant human neuronal
            nicotinic acetylcholine receptors h alpha 2 beta 2, h alpha 2 beta
            4, h alpha 3 beta 2, h alpha 3 beta 4, h alpha 4 beta 2, h alpha 4
            beta 4 and h alpha 7 expressed in Xenopus oocytes
  JOURNAL   J Pharmacol Exp Ther 280 (1), 346-356 (1997)
   PUBMED   8996215
REFERENCE   7  (bases 1 to 6149)
  AUTHORS   Elliott,K.J., Ellis,S.B., Berckhan,K.J., Urrutia,A.,
            Chavez-Noriega,L.E., Johnson,E.C., Velicelebi,G. and Harpold,M.M.
  TITLE     Comparative structure of human neuronal alpha 2-alpha 7 and beta
            2-beta 4 nicotinic acetylcholine receptor subunits and functional
            expression of the alpha 2, alpha 3, alpha 4, alpha 7, beta 2, and
            beta 4 subunits
  JOURNAL   J Mol Neurosci 7 (3), 217-228 (1996)
   PUBMED   8906617
REFERENCE   8  (bases 1 to 6149)
  AUTHORS   Peng,X., Katz,M., Gerzanich,V., Anand,R. and Lindstrom,J.
  TITLE     Human alpha 7 acetylcholine receptor: cloning of the alpha 7
            subunit from the SH-SY5Y cell line and determination of
            pharmacological properties of native receptors and functional alpha
            7 homomers expressed in Xenopus oocytes
  JOURNAL   Mol Pharmacol 45 (3), 546-554 (1994)
   PUBMED   8145738
REFERENCE   9  (bases 1 to 6149)
  AUTHORS   Chini,B., Raimond,E., Elgoyhen,A.B., Moralli,D., Balzaretti,M. and
            Heinemann,S.
  TITLE     Molecular cloning and chromosomal localization of the human alpha
            7-nicotinic receptor subunit gene (CHRNA7)
  JOURNAL   Genomics 19 (2), 379-381 (1994)
   PUBMED   8188270
REFERENCE   10 (bases 1 to 6149)
  AUTHORS   van Bon,B.W.M., Mefford,H.C., de Vries,B.B.A. and Schaaf,C.P.
  TITLE     15q13.3 Recurrent Deletion
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   21290787
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK292069.1, BC037571.1,
            AC068448.10 and BI789267.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On May 17, 2019 this sequence version replaced NM_000746.5.
            
            Summary: The nicotinic acetylcholine receptors (nAChRs) are members
            of a superfamily of ligand-gated ion channels that mediate fast
            signal transmission at synapses. The nAChRs are thought to be
            hetero-pentamers composed of homologous subunits. The proposed
            structure for each subunit is a conserved N-terminal extracellular
            domain followed by three conserved transmembrane domains, a
            variable cytoplasmic loop, a fourth conserved transmembrane domain,
            and a short C-terminal extracellular region. The protein encoded by
            this gene forms a homo-oligomeric channel, displays marked
            permeability to calcium ions and is a major component of brain
            nicotinic receptors that are blocked by, and highly sensitive to,
            alpha-bungarotoxin. Once this receptor binds acetylcholine, it
            undergoes an extensive change in conformation that affects all
            subunits and leads to opening of an ion-conducting channel across
            the plasma membrane. This gene is located in a region identified as
            a major susceptibility locus for juvenile myoclonic epilepsy and a
            chromosomal location involved in the genetic transmission of
            schizophrenia. An evolutionarily recent partial duplication event
            in this region results in a hybrid containing sequence from this
            gene and a novel FAM7A gene. Alternative splicing results in
            multiple transcript variants. [provided by RefSeq, Feb 2012].
            
            Transcript Variant: This variant (1) lacks an in-frame portion of
            the 5' coding region, compared to variant 2. The resulting isoform
            (1) has a shorter N-terminus, compared to isoform 2.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660803.200548.1,
                                           SRR7410571.165085.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA1968968
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000306901.9/ ENSP00000303727.2
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1012              AK292069.1         3-1014
            1013-1348           BC037571.1         782-1117
            1349-1414           AK292069.1         1351-1416
            1415-2019           BC037571.1         1184-1788
            2020-3027           AC068448.10        108686-109693
            3028-3314           BI789267.1         11-297              c
            3315-6149           AC068448.10        109981-112815
FEATURES             Location/Qualifiers
     source          1..6149
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="15"
                     /map="15q13.3"
     gene            1..6149
                     /gene="CHRNA7"
                     /gene_synonym="CHRNA7-2; NACHRA7"
                     /note="cholinergic receptor nicotinic alpha 7 subunit"
                     /db_xref="GeneID:1139"
                     /db_xref="HGNC:HGNC:1960"
                     /db_xref="MIM:118511"
     exon            1..135
                     /gene="CHRNA7"
                     /gene_synonym="CHRNA7-2; NACHRA7"
                     /inference="alignment:Splign:2.1.0"
     CDS             81..1589
                     /gene="CHRNA7"
                     /gene_synonym="CHRNA7-2; NACHRA7"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; cholinergic receptor, nicotinic, alpha
                     polypeptide 7; a7 nicotinic acetylcholine receptor; alpha
                     7 neuronal nicotinic acetylcholine receptor; alpha-7
                     nicotinic cholinergic receptor subunit; neuronal
                     acetylcholine receptor protein, alpha-7 chain; neuronal
                     acetylcholine receptor subunit alpha-7; cholinergic
                     receptor, nicotinic, alpha 7 (neuronal); cholinergic
                     receptor, nicotinic alpha 7"
                     /codon_start=1
                     /product="neuronal acetylcholine receptor subunit alpha-7
                     isoform 1 precursor"
                     /protein_id="NP_000737.1"
                     /db_xref="CCDS:CCDS10027.1"
                     /db_xref="GeneID:1139"
                     /db_xref="HGNC:HGNC:1960"
                     /db_xref="MIM:118511"
                     /translation="MRCSPGGVWLALAASLLHVSLQGEFQRKLYKELVKNYNPLERPV
                     ANDSQPLTVYFSLSLLQIMDVDEKNQVLTTNIWLQMSWTDHYLQWNVSEYPGVKTVRF
                     PDGQIWKPDILLYNSADERFDATFHTNVLVNSSGHCQYLPPGIFKSSCYIDVRWFPFD
                     VQHCKLKFGSWSYGGWSLDLQMQEADISGYIPNGEWDLVGIPGKRSERFYECCKEPYP
                     DVTFTVTMRRRTLYYGLNLLIPCVLISALALLVFLLPADSGEKISLGITVLLSLTVFM
                     LLVAEIMPATSDSVPLIAQYFASTMIIVGLSVVVTVIVLQYHHHDPDGGKMPKWTRVI
                     LLNWCAWFLRMKRPGEDKVRPACQHKQRRCSLASVEMSAVAPPPASNGNLLYIGFRGL
                     DGVHCVPTPDSGVVCGRMACSPTHDEHLLHGGQPPEGDPDLAKILEEVRYIANRFRCQ
                     DESEAVCSEWKFAACVVDRLCLMAFSVFTIICTIGILMSAPNFVEAVSKDFA"
     exon            136..275
                     /gene="CHRNA7"
                     /gene_synonym="CHRNA7-2; NACHRA7"
                     /inference="alignment:Splign:2.1.0"
     exon            276..320
                     /gene="CHRNA7"
                     /gene_synonym="CHRNA7-2; NACHRA7"
                     /inference="alignment:Splign:2.1.0"
     exon            321..430
                     /gene="CHRNA7"
                     /gene_synonym="CHRNA7-2; NACHRA7"
                     /inference="alignment:Splign:2.1.0"
     exon            431..510
                     /gene="CHRNA7"
                     /gene_synonym="CHRNA7-2; NACHRA7"
                     /inference="alignment:Splign:2.1.0"
     exon            511..678
                     /gene="CHRNA7"
                     /gene_synonym="CHRNA7-2; NACHRA7"
                     /inference="alignment:Splign:2.1.0"
     exon            679..873
                     /gene="CHRNA7"
                     /gene_synonym="CHRNA7-2; NACHRA7"
                     /inference="alignment:Splign:2.1.0"
     exon            874..960
                     /gene="CHRNA7"
                     /gene_synonym="CHRNA7-2; NACHRA7"
                     /inference="alignment:Splign:2.1.0"
     exon            961..1070
                     /gene="CHRNA7"
                     /gene_synonym="CHRNA7-2; NACHRA7"
                     /inference="alignment:Splign:2.1.0"
     exon            1071..6149
                     /gene="CHRNA7"
                     /gene_synonym="CHRNA7-2; NACHRA7"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3288..3293
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CHRNA7"
                     /gene_synonym="CHRNA7-2; NACHRA7"
                     /note="hexamer: AATAAA"
     polyA_site      3314
                     /gene="CHRNA7"
                     /gene_synonym="CHRNA7-2; NACHRA7"
                     /note="major polyA site"
     regulatory      5625..5630
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CHRNA7"
                     /gene_synonym="CHRNA7-2; NACHRA7"
                     /note="hexamer: AATAAA"
     polyA_site      5655
                     /gene="CHRNA7"
                     /gene_synonym="CHRNA7-2; NACHRA7"
ORIGIN      
        1 gcctctgtgg ccgcaggcgc aggcccgggc gacagccgag acgtggagcg cgccggctcg
       61 ctgcagctcc gggactcaac atgcgctgct cgccgggagg cgtctggctg gcgctggccg
      121 cgtcgctcct gcacgtgtcc ctgcaaggcg agttccagag gaagctttac aaggagctgg
      181 tcaagaacta caatcccttg gagaggcccg tggccaatga ctcgcaacca ctcaccgtct
      241 acttctccct gagcctcctg cagatcatgg acgtggatga gaagaaccaa gttttaacca
      301 ccaacatttg gctgcaaatg tcttggacag atcactattt acagtggaat gtgtcagaat
      361 atccaggggt gaagactgtt cgtttcccag atggccagat ttggaaacca gacattcttc
      421 tctataacag tgctgatgag cgctttgacg ccacattcca cactaacgtg ttggtgaatt
      481 cttctgggca ttgccagtac ctgcctccag gcatattcaa gagttcctgc tacatcgatg
      541 tacgctggtt tccctttgat gtgcagcact gcaaactgaa gtttgggtcc tggtcttacg
      601 gaggctggtc cttggatctg cagatgcagg aggcagatat cagtggctat atccccaatg
      661 gagaatggga cctagtggga atccccggca agaggagtga aaggttctat gagtgctgca
      721 aagagcccta ccccgatgtc accttcacag tgaccatgcg ccgcaggacg ctctactatg
      781 gcctcaacct gctgatcccc tgtgtgctca tctccgccct cgccctgctg gtgttcctgc
      841 ttcctgcaga ttccggggag aagatttccc tggggataac agtcttactc tctcttaccg
      901 tcttcatgct gctcgtggct gagatcatgc ccgcaacatc cgattcggta ccattgatag
      961 cccagtactt cgccagcacc atgatcatcg tgggcctctc ggtggtggtg acagtgatcg
     1021 tgctgcagta ccaccaccac gaccccgacg ggggcaagat gcccaagtgg accagagtca
     1081 tccttctgaa ctggtgcgcg tggttcctgc gaatgaagag gcccggggag gacaaggtgc
     1141 gcccggcctg ccagcacaag cagcggcgct gcagcctggc cagtgtggag atgagcgccg
     1201 tggcgccgcc gcccgccagc aacgggaacc tgctgtacat cggcttccgc ggcctggacg
     1261 gcgtgcactg tgtcccgacc cccgactctg gggtagtgtg tggccgcatg gcctgctccc
     1321 ccacgcacga tgagcacctc ctgcacggcg ggcaaccccc cgagggggac ccggacttgg
     1381 ccaagatcct ggaggaggtc cgctacattg ccaaccgctt ccgctgccag gacgaaagcg
     1441 aggcggtctg cagcgagtgg aagttcgccg cctgtgtggt ggaccgcctg tgcctcatgg
     1501 ccttctcggt cttcaccatc atctgcacca tcggcatcct gatgtcggct cccaacttcg
     1561 tggaggccgt gtccaaagac tttgcgtaac cacgcctggt tctgtacatg tggaaaactc
     1621 acagatgggc aaggcctttg gcttggcgag atttgggggt gctaatccag gacagcatta
     1681 cacgccacaa ctccagtgtt cccttctggc tgtcagtcgt gttgcttacg gtttctttgt
     1741 tactttaggt agtagaatct cagcactttg tttcatattc tcagatgggc tgatagatat
     1801 ccttggcaca tccgtaccat cggtcagcag ggccactgag tagtcatttt gcccattagc
     1861 ccactgcctg gaaagccctt cggagagctc cccatggctc ctcaccaccg agacagttgg
     1921 ttttgcatgt ctgcatgaag gtctacctga aaattcaaca tttgcttttt gcttgtgtac
     1981 aaacccagat tgaagctaaa ataaaccaga ctcactaaat cctttccaat aattgactgg
     2041 tggaaggaaa acaaaaaaca aaaactaaaa acctcttagc ttttctgcaa ttcaactttt
     2101 tatttttatt tttatttcta tcaaagacgg tagagagaaa cagcttgatg ctgtttctac
     2161 attaaaaaaa aaaaaaaaag acagactgtt ggtcttacta aggatgtttt taccagcctg
     2221 cctgacttct gcaaacctac cctgtcaagg agatcaaagg gacgcaggtt tctgtttatt
     2281 ctgaacaagg gccaggcccc gcggagtgtc tttggtggat cccagataac tcctaggtgc
     2341 tgctctcaga cactgaggag ttgagcaaat ctgttctatt ctgcagaacc cacaggacaa
     2401 ataagagttc tactagaatt aacagcccaa aagaatagct acagctaagt gaagccactt
     2461 acgtgggctt taaaaaaata atgtgttagc tgattcacat gcactggagt taattagtct
     2521 tagaaatgtg tgcatccata caaatgcaca acataaagtg aacatattcc taggcccttt
     2581 ctgcctgtgt cagggccagg aagtagaggc tgggaactct tctggtcccc agtatggcag
     2641 gcgccaggga ggggatggtg tggcccatcc cttctctgga tacctggcca gtggcaggca
     2701 gcagggagga gctggccgac cctcagtgac tgacaagcca gcaattctga gttctggcct
     2761 ttgggagtct gcctgctcca agccagtcca ccccagctgc agccccaaaa gctggctcaa
     2821 agtccttggg tggattcact ggagatgggc aacttaaaac aagagaaact ttaattttta
     2881 aacctaagtg atgatacagc tcttccctta gattatcgcc caggctggag tgcagtggca
     2941 tgatctcagc tcactgcaag ctccacctcc tgggttcatg ccattctcct gcctcagcct
     3001 ccccccgagt aactgggaat acaggcgccc gccaccatgc ctggctaatt ttttgtattt
     3061 ttagtagaga tagggtttca tcatgttagc caggatggtc tcattcttat tctttaatga
     3121 gatcagaggg taattcacca agaaagacct ctcctgttcc attgtgtcat ccaacaactg
     3181 ctcagagctc aaaattatag aaggcttctg agcccctaga gatttttaat ttgcttctaa
     3241 tccctgaggt gggaacatca tgagggaaga tttgattttc agagttaaat aaattgtatg
     3301 tgcttttcca gccatctggc tcactcattt ctgggtaatg cacatgactt tgtttgcact
     3361 ggaggaagat ggaagcttgc gtgtgtgcgg tgtgtgtgtg tgtgtaagtg tgaggtacct
     3421 tgtgtgtgac aagagacctc acttacgaga aagttggtgg atcaggacat tccagcctca
     3481 ggcggcttgg agcaggatca ttcctcagca ggcattcctt ccacatgcta tggatgaacc
     3541 atgcacaaga ttttcggttt tttttttttt ttctgttaca gtgtctttag aaacaagtag
     3601 aagtgttttg atatataaaa ggaatgcttc atttcttatc attatcccaa aattgatccc
     3661 tcccacattt ttgctttaaa aagaaacctt tttggttttg tattttatac aggaacacaa
     3721 aatgcaaaca agttgtgcat attttggact ctcaaataac ttctctgtcc tttaataaag
     3781 taataataag gaatagatgt gcacatagtt agaaaagtat gagtggttaa aaacaacagt
     3841 ctcccatact gcctgttttc tctctcagag gggaccacta atgagcttct aagggcattt
     3901 ctagaaagga agaaaagaag ataagaggcc aaagaaggag ggacgggggt tatggagaga
     3961 gaccttccct ttccagcact aacgctctgt gaagggcgct gcaccttgca ttcttcatgt
     4021 ggtgttttgt cttagaggtc gtcccataat aacatataaa gatctacctc attcctccta
     4081 gtagctgctt agcattccat tatctcagcg gactatcatt tatttgaata gccttttatt
     4141 ggtgaacata tggatacttc cagtttttat ttttatatac aaatctataa ttggcatact
     4201 tgtacataca tcttgacata gttttgtgac taaaacggta taaattccaa cttagaaatg
     4261 gaattgctgg gtttaaggct acatgtattt aaaattttga tagctattgc cagattatct
     4321 tctagaaact gatcgaagga tgtaactcca tcaagagtac aataaaaggc ccaatttctc
     4381 agctttctat aatagcactg ggtattttca aacttaatgt ttttcccaat ataattggtg
     4441 aaacatgata tttacgaata ttattctgat tttggtttat tttgtgtata aaatgggaca
     4501 tcttttaaaa tttttttgta gttttaattt tgatacattt atatatttta cccagttcct
     4561 aattgggtta tttggctttt tcttaatttg ctcatgcact ttgggacatt tttgtcttat
     4621 gtctttaaaa tatttttatc atttgtcttt cgatgttatt tattgatttc cttatttatt
     4681 ggcatagagt ttaaattttt atgttgtcaa tttgtgagta tttttctttg tcgatcgtgg
     4741 gatttgtttc cagtttttaa aagccttcct cattctgaga ttataaatag acttactcgc
     4801 attttcttct gaaactttat ggttttattg gtttaccttc acatcttcag actccctgga
     4861 attcatttgt tgcaggacat gagggaaaaa tccatacttt ttcctcgaaa tggtggtcag
     4921 cagtatcaat aaatatgtaa taatgcattt ctaaaataca gtcatagtgt tttccccagt
     4981 tgttcaaaat gcagccttat ttgtaaacta aatttcttta agtgtttggt tctgtttctg
     5041 ttctatttat ctctatgaat gcctttcatt tctaaagcat tattcatact aatatcagta
     5101 ttttcaagtc aacactccca tcaactctga atctctctct catatgaatc ttttttcaat
     5161 tagagtcaca atgaatttac agattgagtc ttcctattca aaagcatgaa atcttgtgtt
     5221 taagtctaat tttatgtaac ttagtagtgt tttatgattt tgttcactaa ggtgctttga
     5281 atttatagtt tatttctaga tgttttatca tttggctatc attttcaata gaatgattta
     5341 ttccacttta ttttctattt tatcccattg tataagaaag tagttggttt ttatgtatga
     5401 atgttataat caaccacaaa ttgtgattcc tacttgtttc taaggtatac ttttgacttc
     5461 tcctttccaa tgtgtctctt atttctttct tgtatctagt tgatttgaga tcagtgttga
     5521 gtcacagtga ttcaggtggc atgcttatct tgttcgtgac atcagtggga cgttttaatg
     5581 tctcagcgtt taacttgtat gaagacatgt attttccttg ccataataaa aaagtatctg
     5641 tgtgttctca aactattaag actctctggg gaaaatgtag atgttagatt tttgtcaaag
     5701 ccttctcagt gtccagaggg attatcagaa ggtcttttcc tttgctctag tagtatgttt
     5761 aattatatta atagaatact taagaccaaa tcattagtgt attcctgaga tgttctttac
     5821 ttattagtat catgactttg gttatcaata tttataatta agattcatca ttctacatct
     5881 tgcttttatg tgttttttgt ccagatttaa ctttatgctg acttaaagga agaaatatta
     5941 taaaacaaaa acctctatat tctacaatca tctattggtt ttggattatt ttctgcttaa
     6001 gtgtagaaga actagctggg gctgtctggg cctgccgtgg tatatttctg gattttgctc
     6061 atcaggcaag ttaggtcctc gagcccttct agagtccatt ttaatgacac ttttaaaaat
     6121 aaaattaatt ccatccactt ttcaacata
//
