LOCUS       NM_000745               3623 bp    mRNA    linear   PRI 05-OCT-2024
DEFINITION  Homo sapiens cholinergic receptor nicotinic alpha 5 subunit
            (CHRNA5), transcript variant 1, mRNA.
ACCESSION   NM_000745
VERSION     NM_000745.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3623)
  AUTHORS   Zuo,Y., Rose,J.E., Davis,J.M., Behrens,K.A., Golaub,A.A.,
            Chandra,U.U., Aarons,E.K., Morgan-Glover,J.D. and Mukhin,A.G.
  TITLE     Nicotinic Receptor Alpha-5 Subunit Gene Polymorphism is Associated
            With Heavy Smoking Under a Range of Nicotine Dosing Conditions
  JOURNAL   Nicotine Tob Res 26 (10), 1296-1304 (2024)
   PUBMED   38654694
  REMARK    GeneRIF: Nicotinic Receptor Alpha-5 Subunit Gene Polymorphism is
            Associated With Heavy Smoking Under a Range of Nicotine Dosing
            Conditions.
REFERENCE   2  (bases 1 to 3623)
  AUTHORS   Rybnicek,J., Chen,Y., Milic,M., Tio,E.S., McLaurin,J., Hohman,T.J.,
            De Jager,P.L., Schneider,J.A., Wang,Y., Bennett,D.A., Tripathy,S.,
            Felsky,D. and Lambe,E.K.
  TITLE     CHRNA5 links chandelier cells to severity of amyloid pathology in
            aging and Alzheimer's disease
  JOURNAL   Transl Psychiatry 14 (1), 83 (2024)
   PUBMED   38331937
  REMARK    GeneRIF: CHRNA5 links chandelier cells to severity of amyloid
            pathology in aging and Alzheimer's disease.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3623)
  AUTHORS   Li,Q., Li,J., Wang,J., Wang,J., Lu,T., Jia,Y., Sun,H. and Ma,X.
  TITLE     PLEK2 mediates metastasis and invasion via alpha5-nAChR activation
            in nicotine-induced lung adenocarcinoma
  JOURNAL   Mol Carcinog 63 (2), 253-265 (2024)
   PUBMED   37921560
  REMARK    GeneRIF: PLEK2 mediates metastasis and invasion via alpha5-nAChR
            activation in nicotine-induced lung adenocarcinoma.
REFERENCE   4  (bases 1 to 3623)
  AUTHORS   Yang,K., McLaughlin,I., Shaw,J.K., Quijano-Carde,N., Dani,J.A. and
            De Biasi,M.
  TITLE     CHRNA5 gene variation affects the response of VTA dopaminergic
            neurons during chronic nicotine exposure and withdrawal
  JOURNAL   Neuropharmacology 235, 109547 (2023)
   PUBMED   37116611
  REMARK    GeneRIF: CHRNA5 gene variation affects the response of VTA
            dopaminergic neurons during chronic nicotine exposure and
            withdrawal.
REFERENCE   5  (bases 1 to 3623)
  AUTHORS   Valencia-Perez Rea,D., Falfan-Valencia,R., Fricke-Galindo,I.,
            Buendia-Roldan,I., Chavez-Galan,L., Nava-Quiroz,K.J.,
            Alanis-Ponce,J. and Perez-Rubio,G.
  TITLE     The rs16969968 Tobacco Smoking-Related Single-Nucleotide Variant Is
            Associated with Clinical Markers in Patients with Severe COVID-19
  JOURNAL   Int J Mol Sci 24 (12), 9811 (2023)
   PUBMED   37372959
  REMARK    GeneRIF: The rs16969968 Tobacco Smoking-Related Single-Nucleotide
            Variant Is Associated with Clinical Markers in Patients with Severe
            COVID-19.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3623)
  AUTHORS   Eggert,M., Aichinger,E., Pfaffl,M.W., Steinlein,O.K. and Pfob,M.
  TITLE     Nicotinic acetylcholine receptor subunits alpha4 and alpha5
            associated with smoking behaviour and lung cancer are regulated by
            upstream open reading frames
  JOURNAL   PLoS One 8 (7), e66157 (2013)
   PUBMED   23843950
  REMARK    GeneRIF: Nicotinic acetylcholine receptor subunits alpha4 and
            alpha5 associated with smoking behavior and lung cancer are
            regulated by upstream open reading frames.
            Publication Status: Online-Only
REFERENCE   7  (bases 1 to 3623)
  AUTHORS   Anand,R. and Lindstrom,J.
  TITLE     Chromosomal localization of seven neuronal nicotinic acetylcholine
            receptor subunit genes in humans
  JOURNAL   Genomics 13 (4), 962-967 (1992)
   PUBMED   1505988
REFERENCE   8  (bases 1 to 3623)
  AUTHORS   Raimondi,E., Rubboli,F., Moralli,D., Chini,B., Fornasari,D.,
            Tarroni,P., De Carli,L. and Clementi,F.
  TITLE     Chromosomal localization and physical linkage of the genes encoding
            the human alpha 3, alpha 5, and beta 4 neuronal nicotinic receptor
            subunits
  JOURNAL   Genomics 12 (4), 849-850 (1992)
   PUBMED   1572664
REFERENCE   9  (bases 1 to 3623)
  AUTHORS   Chini,B., Clementi,F., Hukovic,N. and Sher,E.
  TITLE     Neuronal-type alpha-bungarotoxin receptors and the alpha
            5-nicotinic receptor subunit gene are expressed in neuronal and
            nonneuronal human cell lines
  JOURNAL   Proc Natl Acad Sci U S A 89 (5), 1572-1576 (1992)
   PUBMED   1542648
REFERENCE   10 (bases 1 to 3623)
  AUTHORS   Eng,C.M., Kozak,C.A., Beaudet,A.L. and Zoghbi,H.Y.
  TITLE     Mapping of multiple subunits of the neuronal nicotinic
            acetylcholine receptor to chromosome 15 in man and chromosome 9 in
            mouse
  JOURNAL   Genomics 9 (2), 278-282 (1991)
   PUBMED   2004777
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC027228.16.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jun 8, 2021 this sequence version replaced NM_000745.3.
            
            Summary: The protein encoded by this gene is a nicotinic
            acetylcholine receptor subunit and a member of a superfamily of
            ligand-gated ion channels that mediate fast signal transmission at
            synapses. These receptors are thought to be heteropentamers
            composed of separate but similar subunits. Defects in this gene
            have been linked to susceptibility to lung cancer type 2
            (LNCR2).[provided by RefSeq, Jun 2010].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038196.2089620.1,
                                           SRR14038195.996036.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000299565.9/ ENSP00000299565.5
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            regulatory uORF        :: PMID: 23843950
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-306               AC027228.16        31189-31494         c
            307-458             AC027228.16        16052-16203         c
            459-503             AC027228.16        10325-10369         c
            504-613             AC027228.16        8591-8700           c
            614-1445            AC027228.16        6378-7209           c
            1446-3623           AC027228.16        1745-3922           c
FEATURES             Location/Qualifiers
     source          1..3623
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="15"
                     /map="15q25.1"
     gene            1..3623
                     /gene="CHRNA5"
                     /gene_synonym="LNCR2"
                     /note="cholinergic receptor nicotinic alpha 5 subunit"
                     /db_xref="GeneID:1138"
                     /db_xref="HGNC:HGNC:1959"
                     /db_xref="MIM:118505"
     exon            1..306
                     /gene="CHRNA5"
                     /gene_synonym="LNCR2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3..32
                     /regulatory_class="uORF"
                     /gene="CHRNA5"
                     /gene_synonym="LNCR2"
                     /experiment="DESCRIPTION:regulatory uORF[PMID:23843950]"
                     /note="this uORF is predicted to encode a 9 aa peptide
                     (PMID:23843950)"
     CDS             201..1607
                     /gene="CHRNA5"
                     /gene_synonym="LNCR2"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; Cholinergic receptor, neuronal nicotinic, alpha
                     polypeptide-5; acetylcholine receptor, nicotinic, alpha 5
                     (neuronal); neuronal nicotinic acetylcholine receptor,
                     alpha5 subunit; cholinergic receptor, nicotinic, alpha 5
                     (neuronal); cholinergic receptor, nicotinic alpha 5"
                     /codon_start=1
                     /product="neuronal acetylcholine receptor subunit alpha-5
                     isoform 1 precursor"
                     /protein_id="NP_000736.2"
                     /db_xref="CCDS:CCDS10304.1"
                     /db_xref="GeneID:1138"
                     /db_xref="HGNC:HGNC:1959"
                     /db_xref="MIM:118505"
                     /translation="MAARGSGPRALRLLLLVQLVAGRCGLAGAAGGAQRGLSEPSSIA
                     KHEDSLLKDLFQDYERWVRPVEHLNDKIKIKFGLAISQLVDVDEKNQLMTTNVWLKQE
                     WIDVKLRWNPDDYGGIKVIRVPSDSVWTPDIVLFDNADGRFEGTSTKTVIRYNGTVTW
                     TPPANYKSSCTIDVTFFPFDLQNCSMKFGSWTYDGSQVDIILEDQDVDKRDFFDNGEW
                     EIVSATGSKGNRTDSCCWYPYVTYSFVIKRLPLFYTLFLIIPCIGLSFLTVLVFYLPS
                     NEGEKICLCTSVLVSLTVFLLVIEEIIPSSSKVIPLIGEYLVFTMIFVTLSIMVTVFA
                     INIHHRSSSTHNAMAPLVRKIFLHTLPKLLCMRSHVDRYFTQKEETESGSGPKSSRNT
                     LEAALDSIRYITRHIMKENDVREVVEDWKFIAQVLDRMFLWTFLFVSIVGSLGLFVPV
                     IYKWANILIPVHIGNANK"
     exon            307..458
                     /gene="CHRNA5"
                     /gene_synonym="LNCR2"
                     /inference="alignment:Splign:2.1.0"
     exon            459..503
                     /gene="CHRNA5"
                     /gene_synonym="LNCR2"
                     /inference="alignment:Splign:2.1.0"
     exon            504..613
                     /gene="CHRNA5"
                     /gene_synonym="LNCR2"
                     /inference="alignment:Splign:2.1.0"
     exon            614..1445
                     /gene="CHRNA5"
                     /gene_synonym="LNCR2"
                     /inference="alignment:Splign:2.1.0"
     exon            1446..3623
                     /gene="CHRNA5"
                     /gene_synonym="LNCR2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1900..1905
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CHRNA5"
                     /gene_synonym="LNCR2"
                     /note="hexamer: AGTAAA"
     polyA_site      1931
                     /gene="CHRNA5"
                     /gene_synonym="LNCR2"
                     /note="major polyA site"
     regulatory      2034..2039
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CHRNA5"
                     /gene_synonym="LNCR2"
                     /note="hexamer: ATTATA"
     polyA_site      2055
                     /gene="CHRNA5"
                     /gene_synonym="LNCR2"
     regulatory      2454..2459
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CHRNA5"
                     /gene_synonym="LNCR2"
                     /note="hexamer: AATAAA"
     regulatory      2458..2463
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CHRNA5"
                     /gene_synonym="LNCR2"
                     /note="hexamer: AATGAA"
     polyA_site      2472
                     /gene="CHRNA5"
                     /gene_synonym="LNCR2"
     polyA_site      2483
                     /gene="CHRNA5"
                     /gene_synonym="LNCR2"
     regulatory      2643..2648
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CHRNA5"
                     /gene_synonym="LNCR2"
                     /note="hexamer: AATAGA"
     polyA_site      2665
                     /gene="CHRNA5"
                     /gene_synonym="LNCR2"
     regulatory      3606..3611
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CHRNA5"
                     /gene_synonym="LNCR2"
                     /note="hexamer: TATAAA"
     polyA_site      3623
                     /gene="CHRNA5"
                     /gene_synonym="LNCR2"
ORIGIN      
        1 acatgcgtcc cgagcccgcc agaagctgct aggctgaggc tgctgtcccg gcgggagctg
       61 tggcgcggag cggcccctct gctgcgtctg ccctcgtttt gtctcacgac tcacactcag
      121 tgctccattc cccaagagtt cgcgttcccc gcgcggcggt cgagaggcgg ctgcccgcgg
      181 tcccgcgcgg gcgcggggcg atggcggcgc gggggtcagg gccccgcgcg ctccgcctgc
      241 tgctcttggt ccagctggtc gcggggcgct gcggtctagc gggcgcggcg ggcggcgcgc
      301 agagaggatt atctgaacct tcttctattg caaaacatga agatagtttg cttaaggatt
      361 tatttcaaga ctacgaaaga tgggttcgtc ctgtggaaca cctgaatgac aaaataaaaa
      421 taaaatttgg acttgcaata tctcaattgg tggatgtgga tgagaaaaat cagttaatga
      481 caacaaacgt ctggttgaaa caggaatgga tagatgtaaa attaagatgg aaccctgatg
      541 actatggtgg aataaaagtt atacgtgttc cttcagactc tgtctggaca ccagacatcg
      601 ttttgtttga taatgcagat ggacgttttg aagggaccag tacgaaaaca gtcatcaggt
      661 acaatggcac tgtcacctgg actccaccgg caaactacaa aagttcctgt accatagatg
      721 tcacgttttt cccatttgac cttcagaact gttccatgaa atttggttct tggacttatg
      781 atggatcaca ggttgatata attctagagg accaagatgt agacaagaga gatttttttg
      841 ataatggaga atgggagatt gtgagtgcaa cagggagcaa aggaaacaga accgacagct
      901 gttgctggta tccgtatgtc acttactcat ttgtaatcaa gcgcctgcct ctcttttata
      961 ccttgttcct tataataccc tgtattgggc tctcattttt aactgtactt gtcttctatc
     1021 ttccttcaaa tgaaggtgaa aagatttgtc tctgcacttc agtacttgtg tctttgactg
     1081 tcttccttct ggttattgaa gagatcatac catcatcttc aaaagtcata cctctaattg
     1141 gagagtatct ggtatttacc atgatttttg tgacactgtc aattatggta accgtcttcg
     1201 ctatcaacat tcatcatcgt tcttcctcaa cacataatgc catggcgcct ttggtccgca
     1261 agatatttct tcacacgctt cccaaactgc tttgcatgag aagtcatgta gacaggtact
     1321 tcactcagaa agaggaaact gagagtggta gtggaccaaa atcttctaga aacacattgg
     1381 aagctgcgct cgattctatt cgctacatta caagacacat catgaaggaa aatgatgtcc
     1441 gtgaggttgt tgaagattgg aaattcatag cccaggttct tgatcggatg tttctgtgga
     1501 cttttctttt cgtttcaatt gttggatctc ttgggctttt tgttcctgtt atttataaat
     1561 gggcaaatat attaatacca gttcatattg gaaatgcaaa taagtgaagc ctcccaaggg
     1621 actgaagtat acatttagtt aacacacata tatctgatgg cacctataaa attatgaaaa
     1681 tgtaagttat gtgttaaatt tagtgcaagc tttaacagac taagttgcta acctcaattt
     1741 atgttaacag atgatccatt tgaacagttg gctgtatgac tgaagtaata actgatgaga
     1801 tacatttgat cttgtaaaaa tagcaaaata ttatctgaac tggactagtg aaaaatctag
     1861 tatttgtatc ctggcaaata atactaattt ataatccaca gtaaagttca tcctttgact
     1921 gtgctggaga attccagttg tatttgaaga ctgattttaa aacttttctg catttggtaa
     1981 aggtatgtaa actttcctgt actcactgag taacagctaa tctttaatat aatattatac
     2041 tgctatattt aaaaagctga ctacttgata taattactta atgtgatgct tgatataata
     2101 attacttaat gtggccgggc acggtggctc acacctgtaa tcccagcact ttgggaggtc
     2161 gaggtgggcg tatcacctga ggttgggagt tcgagaccag cctgaccaac gtggagaaac
     2221 cccgtctcta ctaaaaatat gaaattagcc agggtggtgg tgcacacctg taatcccagc
     2281 tacctgggag gctgcggcag gagaatcgct tgaacccagg tggcggaggt tgcggtgagc
     2341 tgagatcacg ccattgcact ccagcctggg caacaagagc aaaactcagt ctcaaataat
     2401 aataataaca acaacttaat gtgctgctgc ttttccataa ccaacatttt aaaaataaat
     2461 gaaaaacagg aattgggaac tcctttaagg cttactttat tctttagatg cttaattatt
     2521 gtgttaacta tttctgtagc ttagcttcca ctgtaaagtc atacagtaga caactcctgt
     2581 ggacacgcag tagcatatcc ttaacattaa tttcagtcct cttgtccaca tttcccacaa
     2641 ttaatagaac catcttctat ataaattgtg gtagtatctc tttatccttg atcttagaat
     2701 agtcagtcca ctacaattac atgaacccca tttaaaaaac atatttaggg ccgggggcag
     2761 tggctcacat ctgtaatcca agcactttgg gaggctgagg caggtggatc accagaggtt
     2821 aggagttcga gactagcctg accaacatgg tgaagccccg tctctactaa gaatacaaaa
     2881 aattagtcgg gcatggtagc aggcgcctgt agtcccagct actcaagagg ctgaggcagg
     2941 agaatcgctt gaacctggga ggtggaggct gcagtgagcc gagactgcgc cattgcactc
     3001 cagcctggca acaagagcaa aactctgtct caaacatatt ttggtctaaa tcattctgtg
     3061 agaaaacaat cttctaatat gaaacacagt attctaattt ggtatatgca cactgttata
     3121 tacctgtaat atttcagttt tctctccttc attctaacaa ttacaataat agaatcttag
     3181 agttgcaagg gcctttagat gtaatcaatc ttagcctatt actagtacag cgtaaatgat
     3241 ttagtacagt ataatgtatc acagttaaaa cagttaaatt ccatctctaa atgtcaccac
     3301 ttcaggtgtg accaggtagc aaacactgac agaaaccctc gttcaattta gaactcttag
     3361 ctgttgagat cacaaacacc tcatttattt ataataagta acctatctaa gttcaagcca
     3421 atgctctttg gaaggcggag gagaccctat ctaatttgca tttaatcgta ggcaggtgtt
     3481 taatgccatt taatgagtga aagcctggtg tgatgaattt agattgcctg ccagctacct
     3541 accttagttc gtatacatcc ctgatccctc ttatactacc attactgtta cttatgattt
     3601 ttatatataa atttttatcg aca
//
