LOCUS       NM_000743               3015 bp    mRNA    linear   PRI 02-APR-2024
DEFINITION  Homo sapiens cholinergic receptor nicotinic alpha 3 subunit
            (CHRNA3), transcript variant 1, mRNA.
ACCESSION   NM_000743
VERSION     NM_000743.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3015)
  AUTHORS   Chmielowiec,K., Chmielowiec,J., Stronska-Pluta,A., Trybek,G.,
            Smiarowska,M., Suchanecka,A., Wozniak,G., Jaron,A. and Grzywacz,A.
  TITLE     Association of Polymorphism CHRNA5 and CHRNA3 Gene in People
            Addicted to Nicotine
  JOURNAL   Int J Environ Res Public Health 19 (17), 10478 (2022)
   PUBMED   36078193
  REMARK    GeneRIF: Association of Polymorphism CHRNA5 and CHRNA3 Gene in
            People Addicted to Nicotine.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3015)
  AUTHORS   Kitazaki,Y., Ikawa,M., Kishitani,T., Kamisawa,T., Nakane,S.,
            Nakamoto,Y. and Hamano,T.
  TITLE     Progressive Encephalomyelitis with Rigidity and Myoclonus
            (PERM)-like Symptoms Associated with Anti-ganglionic Acetylcholine
            Receptor Antibodies
  JOURNAL   Intern Med 60 (14), 2307-2313 (2021)
   PUBMED   33583893
  REMARK    GeneRIF: Progressive Encephalomyelitis with Rigidity and Myoclonus
            (PERM)-like Symptoms Associated with Anti-ganglionic Acetylcholine
            Receptor Antibodies.
REFERENCE   3  (bases 1 to 3015)
  AUTHORS   Shibao,C.A., Joos,K., Phillips,J.A. 3rd, Cogan,J., Newman,J.H.,
            Hamid,R., Meiler,J., Capra,J., Sheehan,J., Vetrini,F., Yang,Y.,
            Black,B., Diedrich,A., Roberston,D. and Biaggioni,I.
  TITLE     Familial Autonomic Ganglionopathy Caused by Rare CHRNA3 Genetic
            Variants
  JOURNAL   Neurology 97 (2), e145-e155 (2021)
   PUBMED   33947782
  REMARK    GeneRIF: Familial Autonomic Ganglionopathy Caused by Rare CHRNA3
            Genetic Variants.
REFERENCE   4  (bases 1 to 3015)
  AUTHORS   Yi,X., Li,W., Wang,Y., Chen,X., Ye,F., Sun,G. and Chen,J.
  TITLE     The relationship between CHRNA5/A3/B4 gene cluster polymorphisms
            and lung cancer risk: An updated meta-analysis and systematic
            review
  JOURNAL   Medicine (Baltimore) 100 (6), e24355 (2021)
   PUBMED   33578531
  REMARK    GeneRIF: The relationship between CHRNA5/A3/B4 gene cluster
            polymorphisms and lung cancer risk: An updated meta-analysis and
            systematic review.
REFERENCE   5  (bases 1 to 3015)
  AUTHORS   Elliott,K.J., Ellis,S.B., Berckhan,K.J., Urrutia,A.,
            Chavez-Noriega,L.E., Johnson,E.C., Velicelebi,G. and Harpold,M.M.
  TITLE     Comparative structure of human neuronal alpha 2-alpha 7 and beta
            2-beta 4 nicotinic acetylcholine receptor subunits and functional
            expression of the alpha 2, alpha 3, alpha 4, alpha 7, beta 2, and
            beta 4 subunits
  JOURNAL   J Mol Neurosci 7 (3), 217-228 (1996)
   PUBMED   8906617
REFERENCE   6  (bases 1 to 3015)
  AUTHORS   Raimondi,E., Rubboli,F., Moralli,D., Chini,B., Fornasari,D.,
            Tarroni,P., De Carli,L. and Clementi,F.
  TITLE     Chromosomal localization and physical linkage of the genes encoding
            the human alpha 3, alpha 5, and beta 4 neuronal nicotinic receptor
            subunits
  JOURNAL   Genomics 12 (4), 849-850 (1992)
   PUBMED   1572664
REFERENCE   7  (bases 1 to 3015)
  AUTHORS   Eng,C.M., Kozak,C.A., Beaudet,A.L. and Zoghbi,H.Y.
  TITLE     Mapping of multiple subunits of the neuronal nicotinic
            acetylcholine receptor to chromosome 15 in man and chromosome 9 in
            mouse
  JOURNAL   Genomics 9 (2), 278-282 (1991)
   PUBMED   2004777
REFERENCE   8  (bases 1 to 3015)
  AUTHORS   Mihovilovic,M. and Roses,A.D.
  TITLE     Expression of mRNAs in human thymus coding for the alpha 3 subunit
            of a neuronal acetylcholine receptor
  JOURNAL   Exp Neurol 111 (2), 175-180 (1991)
   PUBMED   1989896
REFERENCE   9  (bases 1 to 3015)
  AUTHORS   Papke,R.L. and Heinemann,S.F.
  TITLE     The role of the beta 4-subunit in determining the kinetic
            properties of rat neuronal nicotinic acetylcholine alpha
            3-receptors
  JOURNAL   J Physiol 440, 95-112 (1991)
   PUBMED   1725184
REFERENCE   10 (bases 1 to 3015)
  AUTHORS   Fornasari,D., Chini,B., Tarroni,P. and Clementi,F.
  TITLE     Molecular cloning of human neuronal nicotinic receptor alpha
            3-subunit
  JOURNAL   Neurosci Lett 111 (3), 351-356 (1990)
   PUBMED   2336208
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC006114.1, BC002996.1,
            AC027228.16, BM666615.1 and AI833126.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_000743.4.
            
            Summary: This locus encodes a member of the nicotinic acetylcholine
            receptor family of proteins. Members of this family of proteins
            form pentameric complexes comprised of both alpha and beta
            subunits. This locus encodes an alpha-type subunit, as it contains
            characteristic adjacent cysteine residues. The encoded protein is a
            ligand-gated ion channel that likely plays a role in
            neurotransmission. Polymorphisms in this gene have been associated
            with an increased risk of smoking initiation and an increased
            susceptibility to lung cancer. Alternatively spliced transcript
            variants have been described. [provided by RefSeq, Nov 2009].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC098443.1, SRR1660805.149200.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: downstream AUG is associated
                                                with N-terminal localization
                                                signal
            MANE Ensembl match               :: ENST00000326828.6/
                                                ENSP00000315602.5
            RefSeq Select criteria           :: based on conservation,
                                                expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-493               BC006114.1         300-792
            494-2265            BC002996.1         478-2249
            2266-2266           AC027228.16        956-956
            2267-2671           BC002996.1         2251-2655
            2672-2751           BM666615.1         282-361             c
            2752-3015           AI833126.1         8-271               c
FEATURES             Location/Qualifiers
     source          1..3015
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="15"
                     /map="15q25.1"
     gene            1..3015
                     /gene="CHRNA3"
                     /gene_synonym="BAIPRCK; LNCR2; NACHRA3; PAOD2"
                     /note="cholinergic receptor nicotinic alpha 3 subunit"
                     /db_xref="GeneID:1136"
                     /db_xref="HGNC:HGNC:1957"
                     /db_xref="MIM:118503"
     exon            1..284
                     /gene="CHRNA3"
                     /gene_synonym="BAIPRCK; LNCR2; NACHRA3; PAOD2"
                     /inference="alignment:Splign:2.1.0"
     CDS             203..1720
                     /gene="CHRNA3"
                     /gene_synonym="BAIPRCK; LNCR2; NACHRA3; PAOD2"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; cholinergic receptor, nicotinic, alpha
                     polypeptide 3; neuronal nicotinic acetylcholine receptor,
                     alpha3 subunit; cholinergic receptor, nicotinic, alpha 3
                     (neuronal); cholinergic receptor, nicotinic alpha 3"
                     /codon_start=1
                     /product="neuronal acetylcholine receptor subunit alpha-3
                     isoform 1 precursor"
                     /protein_id="NP_000734.2"
                     /db_xref="CCDS:CCDS10305.1"
                     /db_xref="GeneID:1136"
                     /db_xref="HGNC:HGNC:1957"
                     /db_xref="MIM:118503"
                     /translation="MGSGPLSLPLALSPPRLLLLLLLSLLPVARASEAEHRLFERLFE
                     DYNEIIRPVANVSDPVIIHFEVSMSQLVKVDEVNQIMETNLWLKQIWNDYKLKWNPSD
                     YGGAEFMRVPAQKIWKPDIVLYNNAVGDFQVDDKTKALLKYTGEVTWIPPAIFKSSCK
                     IDVTYFPFDYQNCTMKFGSWSYDKAKIDLVLIGSSMNLKDYWESGEWAIIKAPGYKHD
                     IKYNCCEEIYPDITYSLYIRRLPLFYTINLIIPCLLISFLTVLVFYLPSDCGEKVTLC
                     ISVLLSLTVFLLVITETIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHYRTPT
                     THTMPSWVKTVFLNLLPRVMFMTRPTSNEGNAQKPRPLYGAELSNLNCFSRAESKGCK
                     EGYPCQDGMCGYCHHRRIKISNFSANLTRSSSSESVDAVLSLSALSPEIKEAIQSVKY
                     IAENMKAQNEAKEIQDDWKYVAMVIDRIFLWVFTLVCILGTAGLFLQPLMAREDA"
     exon            285..424
                     /gene="CHRNA3"
                     /gene_synonym="BAIPRCK; LNCR2; NACHRA3; PAOD2"
                     /inference="alignment:Splign:2.1.0"
     exon            425..469
                     /gene="CHRNA3"
                     /gene_synonym="BAIPRCK; LNCR2; NACHRA3; PAOD2"
                     /inference="alignment:Splign:2.1.0"
     exon            470..579
                     /gene="CHRNA3"
                     /gene_synonym="BAIPRCK; LNCR2; NACHRA3; PAOD2"
                     /inference="alignment:Splign:2.1.0"
     exon            580..1591
                     /gene="CHRNA3"
                     /gene_synonym="BAIPRCK; LNCR2; NACHRA3; PAOD2"
                     /inference="alignment:Splign:2.1.0"
     exon            1592..3015
                     /gene="CHRNA3"
                     /gene_synonym="BAIPRCK; LNCR2; NACHRA3; PAOD2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2995..3000
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CHRNA3"
                     /gene_synonym="BAIPRCK; LNCR2; NACHRA3; PAOD2"
                     /note="hexamer: AATAAA"
     polyA_site      3015
                     /gene="CHRNA3"
                     /gene_synonym="BAIPRCK; LNCR2; NACHRA3; PAOD2"
                     /note="major polyA site"
ORIGIN      
        1 actccggatg gttcctgtcc tcccgcgggt ccgagggcgc tggaaaccca gcggcggcga
       61 agcggagagg agccccgcgc gtctccgccc gcacggctcc aggtctgggg tctgcgctgg
      121 agccgcgcgg ggagaggccg tctctgcgac cgccgcgccc gctcccgacc gtccgggtcc
      181 gcggccagcc cggccaccag ccatgggctc tggcccgctc tcgctgcccc tggcgctgtc
      241 gccgccgcgg ctgctgctgc tgctgctgct gtctctgctg ccagtggcca gggcctcaga
      301 ggctgagcac cgtctatttg agcggctgtt tgaagattac aatgagatca tccggcctgt
      361 agccaacgtg tctgacccag tcatcatcca tttcgaggtg tccatgtctc agctggtgaa
      421 ggtggatgaa gtaaaccaga tcatggagac caacctgtgg ctcaagcaaa tctggaatga
      481 ctacaagctg aaatggaacc cctctgacta tggtggggca gagttcatgc gtgtccctgc
      541 acagaagatc tggaagccag acattgtgct gtataacaat gctgttgggg atttccaggt
      601 ggacgacaag accaaagcct tactcaagta cactggggag gtgacttgga tacctccggc
      661 catctttaag agctcctgta aaatcgacgt gacctacttc ccgtttgatt accaaaactg
      721 taccatgaag ttcggttcct ggtcctacga taaggcgaaa atcgatctgg tcctgatcgg
      781 ctcttccatg aacctcaagg actattggga gagcggcgag tgggccatca tcaaagcccc
      841 aggctacaaa cacgacatca agtacaactg ctgcgaggag atctaccccg acatcacata
      901 ctcgctgtac atccggcgcc tgcccttgtt ctacaccatc aacctcatca tcccctgcct
      961 gctcatctcc ttcctcactg tgctcgtctt ctacctgccc tccgactgcg gtgagaaggt
     1021 gaccctgtgc atttctgtcc tcctctccct gacggtgttt ctcctggtga tcactgagac
     1081 catcccttcc acctcgctgg tcatccccct gattggagag tacctcctgt tcaccatgat
     1141 ttttgtaacc ttgtccatcg tcatcaccgt cttcgtgctc aacgtgcact acagaacccc
     1201 gacgacacac acaatgccct catgggtgaa gactgtattc ttgaacctgc tccccagggt
     1261 catgttcatg accaggccaa caagcaacga gggcaacgct cagaagccga ggcccctcta
     1321 cggtgccgag ctctcaaatc tgaattgctt cagccgcgca gagtccaaag gctgcaagga
     1381 gggctacccc tgccaggacg ggatgtgtgg ttactgccac caccgcagga taaaaatctc
     1441 caatttcagt gctaacctca cgagaagctc tagttctgaa tctgttgatg ctgtgctgtc
     1501 cctctctgct ttgtcaccag aaatcaaaga agccatccaa agtgtcaagt atattgctga
     1561 aaatatgaaa gcacaaaatg aagccaaaga gattcaagat gattggaagt atgttgccat
     1621 ggtgattgat cgtatttttc tgtgggtttt caccctggtg tgcattctag ggacagcagg
     1681 attgtttctg caacccctga tggccaggga agatgcataa gcactaagct gtgtgcctgc
     1741 ctgggagact tccttgtgtc agggcaggag gaggctgctt cctagtaaga acgtactttc
     1801 tgttatcaag ctaccagctt tgtttttggc atttcgaggt ttacttattt tccacttatc
     1861 ttggaatcat gcaaaaaaaa aaatgtcaag agtatttatt accgataaat gaacatttaa
     1921 ctagcctttt tggtatggta aagagatgtc aaaatgtgat tctatgtgat tagtatgcta
     1981 tgctatggaa tatacatgta aaaatgtttc cttttagttg ttgaaacaaa actggataga
     2041 aaaatgctgt tcagaaatat gaaaagtcat tcagttatca ctacagatct cccagtaatt
     2101 tttcttattt agcccataat ctctttgaag gtttatacta attcagcaat cccccatcgt
     2161 tacccatttc ttaccatgca tttctcgttc tttactgggt ctaaagggct atgcctccat
     2221 ttcagagagc ttcaactact tctcttgcat acttctaaat tataccatga gaaatcatgc
     2281 ctagttattc attgttaata taactgtctt agtacaccat aaactgggtg gattataaac
     2341 aacagaaact tctcagtttt ggaggttggg aggtccaagg tcaaggcacc agcaaatttg
     2401 gtgtctggtg agggtcctct tcctcaaagg gtgccttcta gctgtgtcct cacatgactg
     2461 aagggactag ctatctctgt ggggtctatt ttataagggc actaacccca ttcatgagag
     2521 cagagccccc atggcctaat cacctttcca aggccccacc ttctatctaa gacaatcacg
     2581 ctgggaatag gtttcaacat atgaattggg ggaggacaca tttggaccac agcatgaacc
     2641 tttagaacag ggtttctcag ccttagcact atggacattt tgggctggat aaatatgtgt
     2701 tggtacagaa tgggggtatc ctgtgcattg taggatcttt agcagtaccc tagcctcaac
     2761 tcactagatg ccaatgacat accttgcttc ttcaccagtt atgataacca agaatgtctc
     2821 cattgttaaa tgttccctta ggagcaaaat tgcccctggt tgagaaacat tgctttagac
     2881 aaattgttaa gagtatcatg tactacactt ctgaaactta acgtgatcat caccactgac
     2941 agatgattca cagagactgt ttgaatcttg tctcactagt ttttcctgtg caaaaataaa
     3001 atggacagaa ttgca
//
