LOCUS       NM_012125               5802 bp    mRNA    linear   PRI 06-APR-2024
DEFINITION  Homo sapiens cholinergic receptor muscarinic 5 (CHRM5), transcript
            variant 1, mRNA.
ACCESSION   NM_012125
VERSION     NM_012125.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5802)
  AUTHORS   Zhang,M.Q., Wan,Y., Jin,Y., Xin,J.B., Zhang,J.C., Xiong,X.Z.,
            Chen,L. and Chen,G.
  TITLE     Cigarette smoking promotes inflammation in patients with COPD by
            affecting the polarization and survival of Th/Tregs through
            up-regulation of muscarinic receptor 3 and 5 expression
  JOURNAL   PLoS One 9 (11), e112350 (2014)
   PUBMED   25375131
  REMARK    GeneRIF: Cigarette smoking may contribute to this imbalance by
            affecting the polarization and survival of Th/Tregs through the
            up-regulation of MR3 and MR5.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5802)
  AUTHORS   Meda,S.A., Jagannathan,K., Gelernter,J., Calhoun,V.D., Liu,J.,
            Stevens,M.C. and Pearlson,G.D.
  TITLE     A pilot multivariate parallel ICA study to investigate differential
            linkage between neural networks and genetic profiles in
            schizophrenia
  JOURNAL   Neuroimage 53 (3), 1007-1015 (2010)
   PUBMED   19944766
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   3  (bases 1 to 5802)
  AUTHORS   Bailey,S.D., Xie,C., Do,R., Montpetit,A., Diaz,R., Mohan,V.,
            Keavney,B., Yusuf,S., Gerstein,H.C., Engert,J.C. and Anand,S.
  CONSRTM   DREAM investigators
  TITLE     Variation at the NFATC2 locus increases the risk of
            thiazolidinedione-induced edema in the Diabetes REduction
            Assessment with ramipril and rosiglitazone Medication (DREAM) study
  JOURNAL   Diabetes Care 33 (10), 2250-2253 (2010)
   PUBMED   20628086
  REMARK    GeneRIF: Observational study of gene-disease association,
            gene-environment interaction, and pharmacogenomic / toxicogenomic.
            (HuGE Navigator)
REFERENCE   4  (bases 1 to 5802)
  AUTHORS   Borroto-Escuela,D.O., Garcia-Negredo,G., Garriga,P., Fuxe,K. and
            Ciruela,F.
  TITLE     The M(5) muscarinic acetylcholine receptor third intracellular loop
            regulates receptor function and oligomerization
  JOURNAL   Biochim Biophys Acta 1803 (7), 813-825 (2010)
   PUBMED   20398705
REFERENCE   5  (bases 1 to 5802)
  AUTHORS   Talmud,P.J., Drenos,F., Shah,S., Shah,T., Palmen,J., Verzilli,C.,
            Gaunt,T.R., Pallas,J., Lovering,R., Li,K., Casas,J.P., Sofat,R.,
            Kumari,M., Rodriguez,S., Johnson,T., Newhouse,S.J., Dominiczak,A.,
            Samani,N.J., Caulfield,M., Sever,P., Stanton,A., Shields,D.C.,
            Padmanabhan,S., Melander,O., Hastie,C., Delles,C., Ebrahim,S.,
            Marmot,M.G., Smith,G.D., Lawlor,D.A., Munroe,P.B., Day,I.N.,
            Kivimaki,M., Whittaker,J., Humphries,S.E. and Hingorani,A.D.
  CONSRTM   ASCOT investigators; NORDIL investigators; BRIGHT Consortium
  TITLE     Gene-centric association signals for lipids and apolipoproteins
            identified via the HumanCVD BeadChip
  JOURNAL   Am J Hum Genet 85 (5), 628-642 (2009)
   PUBMED   19913121
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   6  (bases 1 to 5802)
  AUTHORS   Crespo,P., Xu,N., Daniotti,J.L., Troppmair,J., Rapp,U.R. and
            Gutkind,J.S.
  TITLE     Signaling through transforming G protein-coupled receptors in NIH
            3T3 cells involves c-Raf activation. Evidence for a protein kinase
            C-independent pathway
  JOURNAL   J Biol Chem 269 (33), 21103-21109 (1994)
   PUBMED   8063729
REFERENCE   7  (bases 1 to 5802)
  AUTHORS   Brann,M.R., Ellis,J., Jorgensen,H., Hill-Eubanks,D. and Jones,S.V.
  TITLE     Muscarinic acetylcholine receptor subtypes: localization and
            structure/function
  JOURNAL   Prog Brain Res 98, 121-127 (1993)
   PUBMED   8248499
  REMARK    Review article
REFERENCE   8  (bases 1 to 5802)
  AUTHORS   Gutkind,J.S., Novotny,E.A., Brann,M.R. and Robbins,K.C.
  TITLE     Muscarinic acetylcholine receptor subtypes as agonist-dependent
            oncogenes
  JOURNAL   Proc Natl Acad Sci U S A 88 (11), 4703-4707 (1991)
   PUBMED   1905013
REFERENCE   9  (bases 1 to 5802)
  AUTHORS   Liao,C.F., Themmen,A.P., Joho,R., Barberis,C., Birnbaumer,M. and
            Birnbaumer,L.
  TITLE     Molecular cloning and expression of a fifth muscarinic
            acetylcholine receptor
  JOURNAL   J Biol Chem 264 (13), 7328-7337 (1989)
   PUBMED   2540186
REFERENCE   10 (bases 1 to 5802)
  AUTHORS   Bonner,T.I., Young,A.C., Brann,M.R. and Buckley,N.J.
  TITLE     Cloning and expression of the human and rat m5 muscarinic
            acetylcholine receptor genes
  JOURNAL   Neuron 1 (5), 403-410 (1988)
   PUBMED   3272174
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC009268.10, AK095198.1, BC041805.1 and AC069045.11.
            
            On Jun 2, 2019 this sequence version replaced NM_012125.3.
            
            Summary: The muscarinic cholinergic receptors belong to a larger
            family of G protein-coupled receptors. The functional diversity of
            these receptors is defined by the binding of acetylcholine and
            includes cellular responses such as adenylate cyclase inhibition,
            phosphoinositide degeneration, and potassium channel mediation.
            Muscarinic receptors influence many effects of acetylcholine in the
            central and peripheral nervous system. The clinical implications of
            this receptor are unknown; however, stimulation of this receptor is
            known to increase cyclic AMP levels. [provided by RefSeq, Jul
            2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660809.170464.1,
                                           SRR1660805.181243.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2151119, SAMEA2161674
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000383263.7/ ENSP00000372750.5
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-391               AC009268.10        42124-42514         c
            392-2603            AK095198.1         1-2212
            2604-3130           BC041805.1         1857-2383
            3131-3131           AC069045.11        135186-135186
            3132-3401           BC041805.1         2385-2654
            3402-3402           AC069045.11        135457-135457
            3403-3430           BC041805.1         2656-2683
            3431-5802           AC069045.11        135486-137857
FEATURES             Location/Qualifiers
     source          1..5802
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="15"
                     /map="15q14"
     gene            1..5802
                     /gene="CHRM5"
                     /gene_synonym="HM5"
                     /note="cholinergic receptor muscarinic 5"
                     /db_xref="GeneID:1133"
                     /db_xref="HGNC:HGNC:1954"
                     /db_xref="MIM:118496"
     exon            1..654
                     /gene="CHRM5"
                     /gene_synonym="HM5"
                     /inference="alignment:Splign:2.1.0"
     exon            655..986
                     /gene="CHRM5"
                     /gene_synonym="HM5"
                     /inference="alignment:Splign:2.1.0"
     exon            987..5802
                     /gene="CHRM5"
                     /gene_synonym="HM5"
                     /inference="alignment:Splign:2.1.0"
     CDS             1062..2660
                     /gene="CHRM5"
                     /gene_synonym="HM5"
                     /note="acetylcholine receptor, muscarinic 5"
                     /codon_start=1
                     /product="muscarinic acetylcholine receptor M5"
                     /protein_id="NP_036257.1"
                     /db_xref="CCDS:CCDS10031.1"
                     /db_xref="GeneID:1133"
                     /db_xref="HGNC:HGNC:1954"
                     /db_xref="MIM:118496"
                     /translation="MEGDSYHNATTVNGTPVNHQPLERHRLWEVITIAAVTAVVSLIT
                     IVGNVLVMISFKVNSQLKTVNNYYLLSLACADLIIGIFSMNLYTTYILMGRWALGSLA
                     CDLWLALDYVASNASVMNLLVISFDRYFSITRPLTYRAKRTPKRAGIMIGLAWLISFI
                     LWAPAILCWQYLVGKRTVPLDECQIQFLSEPTITFGTAIAAFYIPVSVMTILYCRIYR
                     ETEKRTKDLADLQGSDSVTKAEKRKPAHRALFRSCLRCPRPTLAQRERNQASWSSSRR
                     STSTTGKPSQATGPSANWAKAEQLTTCSSYPSSEDEDKPATDPVLQVVYKSQGKESPG
                     EEFSAEETEETFVKAETEKSDYDTPNYLLSPAAAHRPKSQKCVAYKFRLVVKADGNQE
                     TNNGCHKVKIMPCPFPVAKEPSTKGLNPNPSHQMTKRKRVVLVKERKAAQTLSAILLA
                     FIITWTPYNIMVLVSTFCDKCVPVTLWHLGYWLCYVNSTVNPICYALCNRTFRKTFKM
                     LLLCRWKKKKVEEKLYWQGNSKLP"
     regulatory      5783..5788
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CHRM5"
                     /gene_synonym="HM5"
                     /note="hexamer: ATTAAA"
     polyA_site      5802
                     /gene="CHRM5"
                     /gene_synonym="HM5"
                     /note="major polyA site"
ORIGIN      
        1 attcatgcca agtacgctca ccgtccagag acatgataaa gccgtacttc cttctggatt
       61 tttacctcac tattcaaaaa acagggtacc agaagcatgg gttacaaaag catgaggagg
      121 acagaagaaa tgccttcatc ttctacagaa aggaagctga tggactgaaa agttcgtatg
      181 gagaaaggaa agcaagagtg caagaacatc catgcttcta gtccagataa tgttttttaa
      241 agcagcttga tttggaagcg tgtgtaattc tgaaatacga acaagcagaa cttttccagg
      301 gggtcaaatg catataggaa caaccaggat ttctcattgc tgaagcattc aatgaatctt
      361 taataagttc tcaacactca gattgtttgt gatcagatca actgctgtta ccagataaag
      421 gcaaaactca cccttctggt tctataatcc tatccaaatg gagtcttcat ctaccgcata
      481 ctatcctaac taatgctaca ggattatcag gaatacagat ataaagacac tgcaagaaat
      541 aaaacactgg caaaataaaa catgaaagga aagtagagaa ggcattttgg gtctcgaagc
      601 ttactatttc cagccagagt tcaaattact gagcttcaaa caaaccactg ccaggaacct
      661 aggaaatcat tctcagcctt gcaacttaaa atatggtaca tggatcagta gcatcaagaa
      721 tactcaggag cttttcagaa atgtgcaatt gtgaaatatg gccaagatgg cggattagaa
      781 gcagcggcag cccgcagctc tcatgcaaag gaatgaaagg tgtgagtgaa tacagcgcct
      841 tcaactgaaa tatccaggtt ctcgctttgg gactaattgg gcaaacaact tgacccacag
      901 aaaacaaaga aaagcagggt ggggtgacgg cccacctggg agcagcacag agccaaagga
      961 acccccacct ccagccaagg gaagcgatgc tggccaagaa gagctgaaat agaaaacagc
     1021 ctagaaccta acactattta ctgtaaaatt tttgcaccag gatggaaggg gattcttacc
     1081 acaatgcaac caccgtcaat ggcaccccag taaatcacca gcctttggaa cgccacaggt
     1141 tgtgggaagt catcaccatt gcagctgtga ctgctgtggt aagcctgatc accattgtgg
     1201 gcaatgtctt ggtcatgatc tccttcaaag tcaacagcca gctcaagaca gttaacaact
     1261 attacctgct cagcttagcc tgtgcagatc tcatcattgg aatcttctcc atgaacctct
     1321 acaccaccta catcctcatg ggacgctggg ctctcgggag tctggcttgt gacctttggc
     1381 ttgcactgga ctacgtggcc agcaacgctt ctgtcatgaa ccttctggtg atcagttttg
     1441 accgttactt ttccatcaca agacccttga catatcgggc caagcgtact ccgaaaaggg
     1501 ctggcatcat gattggcttg gcctggctga tctccttcat cctctgggcc ccagcaatcc
     1561 tctgctggca gtacttggtt gggaagcgga cagttccact ggatgagtgc cagatccagt
     1621 ttctctctga gcccaccatc acttttggca ctgccattgc tgccttctac atccctgttt
     1681 ctgtcatgac catcctctac tgtcgaatct accgggaaac agagaagcga accaaggacc
     1741 tggctgacct ccagggttct gactctgtga ccaaagctga gaagagaaag ccagctcata
     1801 gggctctgtt cagatcctgc ttgcgctgtc ctcgacccac cctggcccag cgggaaagga
     1861 accaggcctc ctggtcatcc tcccgcagga gcacctccac cactgggaag ccatcccaag
     1921 ccactggccc aagcgccaat tgggccaaag ctgagcagct caccacctgt agcagctacc
     1981 cttcctcaga ggatgaggac aagcccgcca ctgaccctgt cctccaagtg gtctacaaga
     2041 gtcagggtaa ggaaagccca ggggaagaat tcagtgctga agagactgag gaaacttttg
     2101 tgaaagctga aactgaaaaa agtgactatg acaccccaaa ctaccttctg tctccagcag
     2161 ctgctcatag acccaagagt cagaaatgtg tggcctataa gttccgattg gtggtaaaag
     2221 ctgacgggaa ccaggagacc aacaatggct gtcacaaggt gaaaatcatg ccctgcccct
     2281 tcccagtggc caaggaacct tcaacgaaag gcctcaatcc caaccccagc catcaaatga
     2341 ccaaacgaaa gagagtggtc ctagtcaaag agaggaaagc agcccagaca ctgagtgcca
     2401 ttctcctggc cttcatcatc acatggaccc cgtataacat catggtcctg gtttctacct
     2461 tctgtgacaa gtgtgtccca gtcaccctgt ggcacttggg ctattggttg tgctatgtca
     2521 atagcactgt caaccccatc tgctatgccc tctgcaacag aaccttcagg aagaccttta
     2581 agatgctgct tctctgccga tggaaaaaga aaaaagtgga agagaagttg tactggcagg
     2641 ggaacagcaa gctaccctga aaagtcaaca actcctctcg aaagaacaat gaccacagtc
     2701 aacatcctct gaggatgagc aagctgattc tggtttgtat attttcaaaa agaagacatc
     2761 tcattttgag tccttgaaga tttttgtaaa ggctcaagtt tggttgccaa atggaagggg
     2821 ccatagctgc agcaattgct gacatattaa atgactcttg cctatgacca aggccatttg
     2881 atgccagggg agtttgccaa tgaagtaaag ggataggctc atggcccttc acaagaggaa
     2941 gcacactggg taacaatgaa cagtgactca gggaacttat gccccttctg taggaaacag
     3001 cagagaccag gtggaaacct tttcctgtgg aaacctgtca tagaattttg tgcaatatgt
     3061 atgtgtctat gaagctgtct tgtgccagtg agaaccagca ggagaatgta caacagtgtc
     3121 acttgttaac gagactgata ttcaacaggc ttctaagaat atgtatcttc ataaactgat
     3181 cactatctat tatgcagcta ttatgtggtc tatactatac tgtggtttgt tttcctgtcc
     3241 ccacatctga gtgaaggtct tgctcttccc tttcatatcc aatgtcaatt ccttgtactc
     3301 actgaaccca tgctgatctc cagggaacca tccttcctct cagaatccag agtctggaag
     3361 gactgaaacc tggtcatacc cagtcctttc aaggggcctc ttttctacta ataaagatgg
     3421 atcaagtctc cacagtcagt tattttagtt tttgccagtg gaaatttttt cttttatcta
     3481 aacagatatt gtgcattcta ttttgtgccc atttgaaccc atttatttat gctctgttct
     3541 taggaagact aattgtgtaa gagcccttca ctggctgaag attttctcca ggctgttgaa
     3601 ttaaggttct tatattgttt tcagagatct tgctttctaa tctcttcaca gggcaaacct
     3661 gagcaaaatg tggaaaacag atttgctttt gaatttcttc cagagccctt ttgcagcctc
     3721 caatttccct tgttcagatc cccagttcac accaagacgc aagcaggtca gctgtgctgc
     3781 ctccaagcag ggggttgtgg ggtgaggtgg gggtagaaag tcttttttta taggtccttc
     3841 aaatcagggc ctaaaaatga cttctttatt cctctccaaa ttcggtcaca aatgagaagg
     3901 ccagaaaagc acacttgagg gtgcgatgaa gctgaaaatg atctgaaagc ttcattttta
     3961 aaaacaaagt gtaggtcata gtaatacata aaaataaaac agttgacatg catgacagca
     4021 tgaggattat taaaaaccac acttaattga aatctctaag tgcgtataaa caaacggaat
     4081 caaaggacct ttgtggacaa aagtccacaa agaagcagta gtgtggtaca cagcaaggat
     4141 tttaaagatg aagcactcct gtcagacatt atttaactcg ttggttacat ctgcagctgg
     4201 acttaaaaat caccagactt catgcgtctt agtgtaggcc gggtcctttt cagtctcatt
     4261 tgctaagagg atggaaaatt agctggttta gttgtcctgg gaattagata tgtacctgag
     4321 tatgggagag cttcagccta ccactgaaga gcatcatcag cctctcacag gatgtcccac
     4381 ggaggcttca gggtagtaga atctcaggca ggacgggact ggtcccttat aaagacttca
     4441 cgttgacatg aaacatcttg taagcagtgc atctgctgag cctttgtaaa gggcaggttg
     4501 acacctgcaa agaattcggt gggaacagta attggctcag ttctccagca cagaggcttt
     4561 cgggcctccg tgatgccaac tgggtgtgac ttcatgagca aacctgctct accagcgcca
     4621 gataaacagt tgagtcttcg tttaaggctt tgattcctga taaagatgaa aaccccttca
     4681 tgagaatagg cttttaagcc catgttgggc attcaccatt cgcagttgag agtgaaacaa
     4741 gctaatttaa acaaaagaac acaaatcggg aaaggatgcc atggagggca ctcctcgcac
     4801 actccgcact tgcttaggac ttggcctttt ttgttcatct tttgtgaaat tgattaagaa
     4861 gtgtattgct gggtgtggtg gctgacacct gtaatcccag cactttggga ggctgaggca
     4921 agaggactgc ttgaccctag gggctcaaga ccagcctggc aacataggga gaccctgtct
     4981 ctacaaaaaa aattttaaat acctgggcat tagcagggca tcgtagtgtg tgcctgtaga
     5041 cccagctact tcagaggttg aagcaggagg atcgcatgag cccaggagtt agaggttgca
     5101 gtgagctatg atcacactac tgcattccag tctaagtgac agagcaagac cctgcctcaa
     5161 aaaaaaaaag agagagagag aggtcaatgc ttcttttttt atcattggtt ttggaaagca
     5221 gccagttttg cagactacct atgcctcttg acatagacaa aaaatatccc aaactactag
     5281 ggacaactag acttctatag aatagcggct ttagtggggt cctcagaatg aatagaccta
     5341 attgtaaact acagggacct gatgggtggg tgggaggcgc atggcctgac ttgagatctt
     5401 aggccttcta tgcccccagt cttccaggct ttgtctatag taaaccatgt atgactttgg
     5461 ctagttacct actggatgct gttactttgc tatcattgta atttgtttcc ccttggctgt
     5521 gaatttgatg acttgtcagg gaatctgttt tccattcaat ttttggttga acaaagcatt
     5581 ttgtcaatat tctcagcttt tgcttcgctc ctttggtgaa tgtcctttga agacaatact
     5641 gatgccagct ctttgtaatt gtgaaatctg tacccaaacc tctggattag aatctccagt
     5701 tgtctactgt aaatactgga attacagcaa aggatatggg gactgggctg cttttctgta
     5761 ttgtataagc actattctag atattaaaga aatttaaccg ca
//
