LOCUS       NM_001375978            9179 bp    mRNA    linear   PRI 28-AUG-2024
DEFINITION  Homo sapiens cholinergic receptor muscarinic 3 (CHRM3), transcript
            variant 3, mRNA.
ACCESSION   NM_001375978 XM_011544041
VERSION     NM_001375978.1
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9179)
  AUTHORS   Smith,J.S., Hilibrand,A.S., Skiba,M.A., Dates,A.N.,
            Calvillo-Miranda,V.G. and Kruse,A.C.
  TITLE     The M3 Muscarinic Acetylcholine Receptor Can Signal through
            Multiple G Protein Families
  JOURNAL   Mol Pharmacol 105 (6), 386-394 (2024)
   PUBMED   38641412
  REMARK    GeneRIF: The M3 Muscarinic Acetylcholine Receptor Can Signal
            through Multiple G Protein Families.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9179)
  AUTHORS   Zhang,X., Yao,S., Bao,P., Du,M., Hu,G., Chu,C., Wang,D., Chen,C.,
            Ma,Q., Jia,H., Sun,Y., Yan,Y., Liao,Y., Niu,Z., Man,Z., Wang,L.,
            Gao,W., Li,H., Zhang,J., Luo,W., Wang,X., Wang,Y. and Mu,J.
  TITLE     Associations of genetic variations in the M3 receptor with salt
            sensitivity, longitudinal changes in blood pressure and the
            incidence of hypertension in Chinese adults
  JOURNAL   J Clin Hypertens (Greenwich) 26 (1), 36-46 (2024)
   PUBMED   38010846
  REMARK    GeneRIF: Associations of genetic variations in the M3 receptor with
            salt sensitivity, longitudinal changes in blood pressure and the
            incidence of hypertension in Chinese adults.
REFERENCE   3  (bases 1 to 9179)
  AUTHORS   Lobbes,L.A., Schutze,M.A., Droeser,R., Arndt,M., Pozios,I.,
            Lauscher,J.C., Hering,N.A. and Weixler,B.
  TITLE     Muscarinic Acetylcholine Receptor M3 Expression and Survival in
            Human Colorectal Carcinoma-An Unexpected Correlation to Guide
            Future Treatment?
  JOURNAL   Int J Mol Sci 24 (9), 8198 (2023)
   PUBMED   37175905
  REMARK    GeneRIF: Muscarinic Acetylcholine Receptor M3 Expression and
            Survival in Human Colorectal Carcinoma-An Unexpected Correlation to
            Guide Future Treatment?
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 9179)
  AUTHORS   Aktan,C., Tekin,F., Oruc,N. and Ozutemiz,O.
  TITLE     CHRM3-Associated miRNAs May Play a Role in Bile Acid-Induced
            Proliferation of H508 Colon Cancer Cells
  JOURNAL   Turk J Gastroenterol 34 (3), 298-307 (2023)
   PUBMED   36919835
  REMARK    GeneRIF: CHRM3-Associated miRNAs May Play a Role in Bile
            Acid-Induced Proliferation of H508 Colon Cancer Cells.
REFERENCE   5  (bases 1 to 9179)
  AUTHORS   Wang,Y., Scheffel,J., Vera,C.A., Liu,W., Gunzel,D.,
            Terhorst-Molawi,D., Maurer,M. and Altrichter,S.
  TITLE     Impaired sweating in patients with cholinergic urticaria is linked
            to low expression of acetylcholine receptor CHRM3 and acetylcholine
            esterase in sweat glands
  JOURNAL   Front Immunol 13, 955161 (2022)
   PUBMED   35967390
  REMARK    GeneRIF: Impaired sweating in patients with cholinergic urticaria
            is linked to low expression of acetylcholine receptor CHRM3 and
            acetylcholine esterase in sweat glands.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 9179)
  AUTHORS   Gutkind,J.S., Novotny,E.A., Brann,M.R. and Robbins,K.C.
  TITLE     Muscarinic acetylcholine receptor subtypes as agonist-dependent
            oncogenes
  JOURNAL   Proc Natl Acad Sci U S A 88 (11), 4703-4707 (1991)
   PUBMED   1905013
REFERENCE   7  (bases 1 to 9179)
  AUTHORS   Goyal,R.K.
  TITLE     Muscarinic receptor subtypes. Physiology and clinical implications
  JOURNAL   N Engl J Med 321 (15), 1022-1029 (1989)
   PUBMED   2674717
  REMARK    Review article
REFERENCE   8  (bases 1 to 9179)
  AUTHORS   Ashkenazi,A., Ramachandran,J. and Capon,D.J.
  TITLE     Acetylcholine analogue stimulates DNA synthesis in brain-derived
            cells via specific muscarinic receptor subtypes
  JOURNAL   Nature 340 (6229), 146-150 (1989)
   PUBMED   2739737
REFERENCE   9  (bases 1 to 9179)
  AUTHORS   Bonner,T.I., Young,A.C., Brann,M.R. and Buckley,N.J.
  TITLE     Cloning and expression of the human and rat m5 muscarinic
            acetylcholine receptor genes
  JOURNAL   Neuron 1 (5), 403-410 (1988)
   PUBMED   3272174
REFERENCE   10 (bases 1 to 9179)
  AUTHORS   Bonner,T.I., Buckley,N.J., Young,A.C. and Brann,M.R.
  TITLE     Identification of a family of muscarinic acetylcholine receptor
            genes
  JOURNAL   Science 237 (4814), 527-532 (1987)
   PUBMED   3037705
  REMARK    Erratum:[Science 1987 Sep 25;237(4822):237]
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC099564.2, AL357499.10,
            AL513543.14, AC104460.2 and AL356361.21.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 7, 2019 this sequence version replaced XM_011544041.2.
            
            Summary: The muscarinic cholinergic receptors belong to a larger
            family of G protein-coupled receptors. The functional diversity of
            these receptors is defined by the binding of acetylcholine and
            includes cellular responses such as adenylate cyclase inhibition,
            phosphoinositide degeneration, and potassium channel mediation.
            Muscarinic receptors influence many effects of acetylcholine in the
            central and peripheral nervous system. The muscarinic cholinergic
            receptor 3 controls smooth muscle contraction and its stimulation
            causes secretion of glandular tissue. Alternative promoter use and
            alternative splicing results in multiple transcript variants that
            have different tissue specificities. [provided by RefSeq, Dec
            2016].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660809.203065.1,
                                           SRR1803617.278681.1 [ECO:0000332]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000676153.1/ ENSP00000502667.1
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-660               AC099564.2         25090-25749
            661-759             AC099564.2         131231-131329
            760-868             AC099564.2         184163-184271
            869-931             AL357499.10        12283-12345         c
            932-1034            AL513543.14        35618-35720
            1035-1161           AC104460.2         66612-66738         c
            1162-9179           AL356361.21        15444-23461
FEATURES             Location/Qualifiers
     source          1..9179
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1q43"
     gene            1..9179
                     /gene="CHRM3"
                     /gene_synonym="EGBRS; HM3; m3AChR; PBS"
                     /note="cholinergic receptor muscarinic 3"
                     /db_xref="GeneID:1131"
                     /db_xref="HGNC:HGNC:1952"
                     /db_xref="MIM:118494"
     exon            1..660
                     /gene="CHRM3"
                     /gene_synonym="EGBRS; HM3; m3AChR; PBS"
                     /inference="alignment:Splign:2.1.0"
     exon            661..759
                     /gene="CHRM3"
                     /gene_synonym="EGBRS; HM3; m3AChR; PBS"
                     /inference="alignment:Splign:2.1.0"
     exon            760..868
                     /gene="CHRM3"
                     /gene_synonym="EGBRS; HM3; m3AChR; PBS"
                     /inference="alignment:Splign:2.1.0"
     exon            869..931
                     /gene="CHRM3"
                     /gene_synonym="EGBRS; HM3; m3AChR; PBS"
                     /inference="alignment:Splign:2.1.0"
     exon            932..1034
                     /gene="CHRM3"
                     /gene_synonym="EGBRS; HM3; m3AChR; PBS"
                     /inference="alignment:Splign:2.1.0"
     exon            1035..1161
                     /gene="CHRM3"
                     /gene_synonym="EGBRS; HM3; m3AChR; PBS"
                     /inference="alignment:Splign:2.1.0"
     exon            1162..9179
                     /gene="CHRM3"
                     /gene_synonym="EGBRS; HM3; m3AChR; PBS"
                     /inference="alignment:Splign:2.1.0"
     CDS             1181..2953
                     /gene="CHRM3"
                     /gene_synonym="EGBRS; HM3; m3AChR; PBS"
                     /note="m3 muscarinic receptor; acetylcholine receptor,
                     muscarinic 3"
                     /codon_start=1
                     /product="muscarinic acetylcholine receptor M3"
                     /protein_id="NP_001362907.1"
                     /db_xref="CCDS:CCDS1616.1"
                     /db_xref="GeneID:1131"
                     /db_xref="HGNC:HGNC:1952"
                     /db_xref="MIM:118494"
                     /translation="MTLHNNSTTSPLFPNISSSWIHSPSDAGLPPGTVTHFGSYNVSR
                     AAGNFSSPDGTTDDPLGGHTVWQVVFIAFLTGILALVTIIGNILVIVSFKVNKQLKTV
                     NNYFLLSLACADLIIGVISMNLFTTYIIMNRWALGNLACDLWLAIDYVASNASVMNLL
                     VISFDRYFSITRPLTYRAKRTTKRAGVMIGLAWVISFVLWAPAILFWQYFVGKRTVPP
                     GECFIQFLSEPTITFGTAIAAFYMPVTIMTILYWRIYKETEKRTKELAGLQASGTEAE
                     TENFVHPTGSSRSCSSYELQQQSMKRSNRRKYGRCHFWFTTKSWKPSSEQMDQDHSSS
                     DSWNNNDAAASLENSASSDEEDIGSETRAIYSIVLKLPGHSTILNSTKLPSSDNLQVP
                     EEELGMVDLERKADKLQAQKSVDDGGSFPKSFSKLPIQLESAVDTAKTSDVNSSVGKS
                     TATLPLSFKEATLAKRFALKTRSQITKRKRMSLVKEKKAAQTLSAILLAFIITWTPYN
                     IMVLVNTFCDSCIPKTFWNLGYWLCYINSTVNPVCYALCNKTFRTTFKMLLLCQCDKK
                     KRRKQQYQQRQSVIFHKRAPEQAL"
     polyA_site      5050
                     /gene="CHRM3"
                     /gene_synonym="EGBRS; HM3; m3AChR; PBS"
     regulatory      9158..9163
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CHRM3"
                     /gene_synonym="EGBRS; HM3; m3AChR; PBS"
                     /note="hexamer: AATAAA"
     polyA_site      9179
                     /gene="CHRM3"
                     /gene_synonym="EGBRS; HM3; m3AChR; PBS"
                     /note="major polyA site"
ORIGIN      
        1 acaccagctc tctcagtccg gggaggagga ggagcaggag gaacgcgagg aggaaggaga
       61 ggaggagcgg ccagcagtag ccacgaccgc caccaccagg cagaggaaga gttcgtgggg
      121 aggaaaagac ctctcctccc ccttggagcg ccttctctct gcttttggag aaagggaata
      181 cacgataaag aaggagacgg aaagaagaga aaaagtgagg cgggagacag agggaaaagg
      241 gcgtgaacag aaagggccgg agcgtgcagg ggagcacagg gcgcgggggc ggcactgccg
      301 agccgggagc gctgccgctt gggcaggtgc cgcggccgct gcccggaggc ggcatgtgac
      361 gcgcggccgc agctgcccgc gggcggagcg ctctcagacc ccggagcgca caccgcgggg
      421 ccatcggtgc catcgcggat ctccaggctc ctcatcagtc cgccggggcc gcagcagcgc
      481 ttctgggaag acgggcgatg aactgaaggg cggctccggg caggggggca cgatcttaag
      541 gacagtcgct ccctgaacgc ggagccggag gagacgaagg gaaggtggag cggacgccac
      601 ccgcgcaccg ggcaggcgcg gagaccggcg tgggacagcc acctggagcg cagctgccag
      661 aaagaaggac tttgctgctt tgggccagga tctgaactta ggtgtaaacc attgccctgg
      721 cagagggaac ctacccagtc cattgctgcc tgctacaaga tatgaacagt aatggcacat
      781 attttggtta tgagtcactc agaggactgt ggattgaatg aactgtatcc atccccatca
      841 tgatgtacag aaccaagtct cttcactaca cttgtgttct gattagtggc caaataaatg
      901 acagtcagaa cttcagctaa ggtacaataa ggttttgctg tggcgtggca cctggtctct
      961 ttctagaagg aaagttcaac atacagcaca attctggaca cattgtattg gtttgatgct
     1021 cctacctgga acaggtggag ctggtctctt gggcagcctg acatctggtc cactcctctg
     1081 tcctatgccg ggatcatcat gaccgtagag attatgtcac tgttttgcat ccttgttaca
     1141 taactcagtt cctggtagat tactatgtca gagagtcaca atgaccttgc acaataacag
     1201 tacaacctcg cctttgtttc caaacatcag ctcctcctgg atacacagcc cctccgatgc
     1261 agggctgccc ccgggaaccg tcactcattt cggcagctac aatgtttctc gagcagctgg
     1321 caatttctcc tctccagacg gtaccaccga tgaccctctg ggaggtcata ccgtctggca
     1381 agtggtcttc atcgctttct taacgggcat cctggccttg gtgaccatca tcggcaacat
     1441 cctggtaatt gtgtcattta aggtcaacaa gcagctgaag acggtcaaca actacttcct
     1501 cttaagcctg gcctgtgccg atctgattat cggggtcatt tcaatgaatc tgtttacgac
     1561 ctacatcatc atgaatcgat gggccttagg gaacttggcc tgtgacctct ggcttgccat
     1621 tgactacgta gccagcaatg cctctgttat gaatcttctg gtcatcagct ttgacagata
     1681 cttttccatc acgaggccgc tcacgtaccg agccaaacga acaacaaaga gagccggtgt
     1741 gatgatcggt ctggcttggg tcatctcctt tgtcctttgg gctcctgcca tcttgttctg
     1801 gcaatacttt gttggaaaga gaactgtgcc tccgggagag tgcttcattc agttcctcag
     1861 tgagcccacc attacttttg gcacagccat cgctgctttt tatatgcctg tcaccattat
     1921 gactatttta tactggagga tctataagga aactgaaaag cgtaccaaag agcttgctgg
     1981 cctgcaagcc tctgggacag aggcagagac agaaaacttt gtccacccca cgggcagttc
     2041 tcgaagctgc agcagttacg aacttcaaca gcaaagcatg aaacgctcca acaggaggaa
     2101 gtatggccgc tgccacttct ggttcacaac caagagctgg aaacccagct ccgagcagat
     2161 ggaccaagac cacagcagca gtgacagttg gaacaacaat gatgctgctg cctccctgga
     2221 gaactccgcc tcctccgacg aggaggacat tggctccgag acgagagcca tctactccat
     2281 cgtgctcaag cttccgggtc acagcaccat cctcaactcc accaagttac cctcatcgga
     2341 caacctgcag gtgcctgagg aggagctggg gatggtggac ttggagagga aagccgacaa
     2401 gctgcaggcc cagaagagcg tggacgatgg aggcagtttt ccaaaaagct tctccaagct
     2461 tcccatccag ctagagtcag ccgtggacac agctaagact tctgacgtca actcctcagt
     2521 gggtaagagc acggccactc tacctctgtc cttcaaggaa gccactctgg ccaagaggtt
     2581 tgctctgaag accagaagtc agatcactaa gcggaaaagg atgtccctgg tcaaggagaa
     2641 gaaagcggcc cagaccctca gtgcgatctt gcttgccttc atcatcactt ggaccccata
     2701 caacatcatg gttctggtga acaccttttg tgacagctgc atacccaaaa ccttttggaa
     2761 tctgggctac tggctgtgct acatcaacag caccgtgaac cccgtgtgct atgctctgtg
     2821 caacaaaaca ttcagaacca ctttcaagat gctgctgctg tgccagtgtg acaaaaaaaa
     2881 gaggcgcaag cagcagtacc agcagagaca gtcggtcatt tttcacaagc gcgcacccga
     2941 gcaggccttg tagaatgagg ttgtatcaat agcagtgaca aaacgcacac atcaacccac
     3001 agaccttagg aggaggaagg cgagggcggg gtgacttctg gtgatgataa aaatggtttt
     3061 atcacccaga tgtgaaagaa gctgcctgtt tactgatcca ttgaataaac ccattttaat
     3121 agaaaaagtc aataccaatt cagcaaaaag aaaaaaaaaa catactactg aatataaaga
     3181 aatttattct gaaatagact ttacgtgttt ttttcttaaa gaggagaaaa atattgcttg
     3241 acggcaatta tatacccaaa gtgatttgcc tgggtccttt aattcccatt agctttggaa
     3301 tctcagatga gcatagctga cccagttccc acattcttcc caaggatccc aaaagtggga
     3361 atccagaccc caagtggaac actgcaggct tacgaatctg tggttccaaa attatttcat
     3421 acgttgcaaa gctgaatctt cttgtcccaa tagagcttcc tgtcttttct ttggtgtgtt
     3481 gttaaactct atttgtggac ttgattcttg attcttgcaa agtactgttt tgtgcagttc
     3541 aagtttcgta caaataaaaa tacttaagta tatatatatg tgtgagttct gcacgcacac
     3601 acatagtgta tataatatca tgggaaacac tgaactggca aattattcct gcaacatacg
     3661 ctttcagtac tttggtaact gaagttctct aggatcctaa tgcaacatta acgtgaaata
     3721 agcccagtgt aatgtttttg aaaccagggc tgttttccac agagagcagc caggccttcc
     3781 cagcaggtct gtgcagagcg gacaggctcg tgagtcagct gagcgccgtg gcttcgccag
     3841 acttggtgtt aagcaacctc ctttgttgat gtctcaacag agctaaatcg gggcccctct
     3901 gagctcaaag aatgaaccac atccacacgt ttgaatttaa tcatctaaat ctgaatgttt
     3961 cagaacaaaa tttctgctat ctaaactgct tgaaactcaa taatagtgtc acgtttgaat
     4021 gtcatacaca gcaatatata tatatgtgta tatatatata tatggcaaag caaaaaaaaa
     4081 aacatggtaa gagagaatga aggagaacat tgtgtttgat tcttgctgaa tggcaccttc
     4141 tcaaagaaaa tagggcttgc acctttgtta atcagctgtg gccagtgctt tctggtgttc
     4201 attgtgtaac cttcacccag gaataggtga ggttttagga agttacatgt cctctgaaga
     4261 aagaattaca ctctgaaaag taatgcttca aattgatttc cttacctttt gggaaaaaaa
     4321 aaaaattgtt tttttgcatt ctcccttgaa ttgaccaaaa tgttaactgt ttcatttggg
     4381 gaggggatgg ggtgctgcca tcattgtcgt tgttgttgct gctgtagctg ttggggtttc
     4441 ttttcctgtt gccggggctg tttggggaga gggaggggag ggaggtggga gggccgcgga
     4501 gatatcttcc ctttgtacag ggcattcgtg ttgtgaaccc agagctgggt agaagctgct
     4561 tttgtattca gtgtgaggtg gtgtttacag acgactttga caacagtaga agtgtactca
     4621 gtggtgtctg tgtatctgaa ctatttaatt tcgtgttatg tttatatgca gaaatattta
     4681 tggatactac accaagtgtt tatttaatgt tgataaatat gactcttcag tcgtcagcca
     4741 tggtgtcctt tcaaagtgat tctttaaggt ccacttgagc aatgaataga gtatattgga
     4801 gctttcctgt ggctaagaag aagaaacatg tcatcctgtt ggcatcacca agcacctaac
     4861 tctttctagg taataaaaag tcaacggtca cttcaacgta aatgtttaaa accactcttt
     4921 agttaaatct gatgggaact gaacaaagct ccaccattgt gtctgaaatc attttacaca
     4981 gggaaaaaaa aaaacaatca gcataattgt aagaatgatt tttttgggcc cacattcaac
     5041 caaagaacaa gaaagaaaca gacgattttc taatatgtac acagcaaact acagaatgtc
     5101 atattatcca cctgtgaaac atctagtaag caattgcatc tatattttat ttctcaagtt
     5161 ggcctggcac taataaatat gtgaaagctt attcaagtgt cccagtactt cagcacttcc
     5221 catccttttg gaaactccta tcttgcctcc ctcccaaaag ggctgtttct tttattaaaa
     5281 aaaaaaaaga aatagaatta aatttgagaa aaggagaaac cctgaaattt aacagaactt
     5341 tgagagtcac ttccaatctc cttaaacatg attatctcct tatccttgtc cttttgtgat
     5401 gctaataata gcagaaaagg aacaaatcca agactctagg tctccctttt ttgttttact
     5461 ggttttatta tagaattgtt tttaagcctc ttatggatga aagctgcatt taaatactat
     5521 ctactgattt tatagaaagg tatgtccatt tcttgggtca ctttcagaga aagaaaacaa
     5581 gcaaaatagg ttcttttgaa tatgaataat ttaaaagcat aattgaagat gtagtgagta
     5641 tttataattg gaccttagtt acacgtgcat ttctcaaagg gcttgtcatt acaggagata
     5701 ttcagcactt acctaagatc aaaatgtctt catcccaaat tgggaatcga attatttcat
     5761 gccatatgca tgaggtttgc agacatttca agattagcca ctttgcttga aagaagaaaa
     5821 tgatcaaacc taaaataaac tacacatgga gaatctctct ctctctctct gcctctctct
     5881 ctctctttct ccctcttctt ccttctctct ctctcacaag cacacacaca cacactcaat
     5941 ctccattctc caggcattgc cagatggtta ggagaagtga gctctctctg gtcccctaga
     6001 tccctcggct ctgctctcct ttgcccatca gctccctgtg gcctctatgc tagtcctccc
     6061 tattctccta gtcacccttt gcctcttaag cccagccttg actctctaca tatttttcat
     6121 ttctctttac cctttagtgt acaatagttg tagactctga cggaatgaaa acaaacaaga
     6181 agcctaagcg ttagaccctg ctgtggctaa agatctagaa ggcatatcag ttttctctgt
     6241 caggcttcct acgttgccct cgtgcagggc tcattgctct gcagagcgct ggaagcccct
     6301 tcatgtatcg agagagaagc tggagcacag agctctgatg cacagttggc tgtttcagct
     6361 ctgactcagg gaggaaagaa ggatgccaag gctctgtcac agcggagaag tctaatacca
     6421 gagtggcaca gagaaggatt ccagggatca gtcagggtgt cctgtaccta tctgagtaga
     6481 gatgattcaa ggatggggac atattccagg aaattgcaca atcttcccct ggcagcttca
     6541 gaggacgagt gaaaatagtg cagtatagtc aaagaagcaa tgaatttagg atgaggaacc
     6601 tgaagtttct ggaccagttt ggtcactgct tagttacatg tccttaggaa tgcttgtcac
     6661 ttaacctaaa ctttctgggt tttatcctca ttgctcaaac taagcaacaa caccgaagct
     6721 tctctaattt gccttctgat gccaaatcgg tcaaaactga ggtcagcagc taccatgacg
     6781 aaagtaaaat ggtatattgg gtgaatgttt gaaaatttca gattttatat tctgtaaagt
     6841 tatttactta ctctatttca acaccacaaa atcttgaaat ttggaaggta accaaggaag
     6901 ctttgaaatt tgttggcaaa cccggagtag tatggtttca aataccactc caacccagca
     6961 cctgaggtcg ggcaaactca caaaatagag tttctaggta gcaggtgttt gctttggttt
     7021 tgcaatagca tttctcataa cagaactgaa actgtataga ttacaacact ccaaagttat
     7081 ttttttttaa cctaaagaac cctacttcta gcacctcctc tgaaagaata ttttgctcca
     7141 ataccacgtg caacaaactt atttagacag tatcaactct agcccttaat aggttcaagg
     7201 acaagtttac tgcttccttt ctacacgctt aagttacact ctcttctcct gttctgatga
     7261 ctctaaatct tcagcctctc ccagcattaa tgtgtgagtc aacattgcag acctgagaaa
     7321 ggtttctaga atggtaaccc aatgttcttt agaactggag aattccattt tccttttctg
     7381 gctttagtgt taatgtctgg gaagggaggc tcatgataag aaaataaaaa tgagaaaaga
     7441 agatctagtt cataaaatga ctatacccca cgtaagtttg taactgcaca gtgttttgtt
     7501 gtacggctca gaaatgagca agtgttagta ttattaggca cacatcacgt tcacagcttc
     7561 gcgcagagac ggatagtgta tttggatata tggaagtcat ccaaatgcgt tgctagaata
     7621 tcaggcctat gtttgttctg gtagaaccac agagactcac acaaagttct gaccctacca
     7681 ctctggtctg ggctgagacc accccaaaaa atcatctaga gaatcaaagg ggctcacatc
     7741 tcagttgcat tctatttaaa agataaaggg gccatactct ccactgcaaa gaagcattag
     7801 taaaacttcc cgcacccaaa ttataatccg gcaaccctag cttcagctaa aaagattatc
     7861 cccagaatct aaaagcttac tattgttctt tcttggagtc caaaatgctt agcagttctg
     7921 cagttcacat acaaaactca aaactctttt tatcctgtat tctgtgagtt ccttgatgat
     7981 agtaattggg tcttattcat cactctgtat cctgtggctc acacagaatc tggcaaattg
     8041 catatattca ataaatgttg aatatctaat aacctcctta ttaatataac aaatattatc
     8101 aacattctgt gcacatcaat gtcccatgct gctactgtag tcaggagtta tttggctggg
     8161 aagtggaaag tgttcacaaa catgatgctt atctaataaa atatcactga gcaataagga
     8221 gaaatatttt aaatagattt gaagttgtga acaaataatt tagagtccaa agaggaaaaa
     8281 gaaaattaac tctgtttttt atccctagaa ctcagaaact ttactggatt ggtcaacaaa
     8341 gacaaacttt ttattgtata aaacagtaga attcatggaa gggataattc ttttggaaca
     8401 ggcttctcgg ttacttgcct tagtaaaaga agtatctgga gggcctggag agttcataaa
     8461 catagttttc tgtgcaagtg aagatctgta gtttggctta aaaatccttg catgtagttt
     8521 tgcttggcgt ggaaagttat taggagacaa tcctttatcc tgttctgcaa gcaatcaaac
     8581 acatgatctc ccttctagcc attcctcatc cagtacatgg gcatttccag cctcctacat
     8641 gtaacacttc aaacttcctc tgggcgtctg cttagagcta tgtgatggcc acagtttggg
     8701 caaagccctt ctttctttta aatataggaa ctgttaccaa gtttggtgca ggcttgagga
     8761 ataattacat aaaaatgggc ttccacgatg gtgaatcagc atgctgattt ctcttggcag
     8821 gaagctctca gggaaatgtc tgtgaatcat ttaaagatgc tctctggtgg atagagaaac
     8881 cgcagtccaa aaacagatgg gtgtatattt gctttgtaag caaagggcat gtatcatact
     8941 cgcatctaaa acagtgtcca catctttgcc aaaggcactg aattttacct ttttttatta
     9001 cctcgtggcc agcacttcct cagtaggcat ttcaggtgtg tcacaaacca tgttcatttt
     9061 taatcttgaa aaagtcaatc gtctgtagtc tgtactataa aatgatgcct gcaactttgt
     9121 ggtgaatatt atccccaagt taacagtgaa ataagtaaat aaaactatta ttgaacaaa
//
