LOCUS       NM_001006632            5641 bp    mRNA    linear   PRI 10-JUN-2024
DEFINITION  Homo sapiens cholinergic receptor muscarinic 2 (CHRM2), transcript
            variant 7, mRNA.
ACCESSION   NM_001006632
VERSION     NM_001006632.3
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5641)
  AUTHORS   Fasciani,I., Petragnano,F., Wang,Z., Edwards,R., Telugu,N.,
            Pietrantoni,I., Zabel,U., Zauber,H., Grieben,M., Terzenidou,M.E.,
            Di Gregorio,J., Pellegrini,C., Santini,S. Jr., Taddei,A.R.,
            Pohl,B., Aringhieri,S., Carli,M., Aloisi,G., Marampon,F.,
            Charlesworth,E., Roman,A., Diecke,S., Flati,V., Giorgi,F.,
            Amicarelli,F., Tobin,A.B., Scarselli,M., Tokatlidis,K., Rossi,M.,
            Lohse,M.J., Annibale,P. and Maggio,R.
  TITLE     The C-terminus of the prototypical M2 muscarinic receptor localizes
            to the mitochondria and regulates cell respiration under stress
            conditions
  JOURNAL   PLoS Biol 22 (4), e3002582 (2024)
   PUBMED   38683874
  REMARK    GeneRIF: The C-terminus of the prototypical M2 muscarinic receptor
            localizes to the mitochondria and regulates cell respiration under
            stress conditions.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5641)
  AUTHORS   Guerriero,C., Fanfarillo,R., Mancini,P., Sterbini,V.,
            Guarguaglini,G., Sforna,L., Michelucci,A., Catacuzzeno,L. and
            Tata,A.M.
  TITLE     M2 muscarinic receptors negatively modulate cell migration in human
            glioblastoma cells
  JOURNAL   Neurochem Int 174, 105673 (2024)
   PUBMED   38185384
  REMARK    GeneRIF: M2 muscarinic receptors negatively modulate cell migration
            in human glioblastoma cells.
REFERENCE   3  (bases 1 to 5641)
  AUTHORS   Refisch,A., Komatsuzaki,S., Ungelenk,M., Chung,H.Y., Schumann,A.,
            Schilling,S.S., Jantzen,W., Schroder,S., Muhleisen,T.W.,
            Nothen,M.M., Hubner,C.A. and Bar,K.J.
  TITLE     Associations of common genetic risk variants of the muscarinic
            acetylcholine receptor M2 with cardiac autonomic dysfunction in
            patients with schizophrenia
  JOURNAL   World J Biol Psychiatry 24 (1), 1-11 (2023)
   PUBMED   35172679
REFERENCE   4  (bases 1 to 5641)
  AUTHORS   Kim,M., Hong,S., Yankeelov,T.E., Yeh,H.C. and Liu,Y.L.
  TITLE     Deep learning-based classification of breast cancer cells using
            transmembrane receptor dynamics
  JOURNAL   Bioinformatics 38 (1), 243-249 (2021)
   PUBMED   34390568
  REMARK    GeneRIF: Deep learning-based classification of breast cancer cells
            using transmembrane receptor dynamics.
REFERENCE   5  (bases 1 to 5641)
  AUTHORS   Katayama,K., Suzuki,K., Suno,R., Kise,R., Tsujimoto,H., Iwata,S.,
            Inoue,A., Kobayashi,T. and Kandori,H.
  TITLE     Vibrational spectroscopy analysis of ligand efficacy in human M2
            muscarinic acetylcholine receptor (M2R)
  JOURNAL   Commun Biol 4 (1), 1321 (2021)
   PUBMED   34815515
  REMARK    GeneRIF: Vibrational spectroscopy analysis of ligand efficacy in
            human M2 muscarinic acetylcholine receptor (M2R).
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 5641)
  AUTHORS   van Koppen,C.J. and Nathanson,N.M.
  TITLE     Site-directed mutagenesis of the m2 muscarinic acetylcholine
            receptor. Analysis of the role of N-glycosylation in receptor
            expression and function
  JOURNAL   J Biol Chem 265 (34), 20887-20892 (1990)
   PUBMED   2249995
REFERENCE   7  (bases 1 to 5641)
  AUTHORS   Goyal,R.K.
  TITLE     Muscarinic receptor subtypes. Physiology and clinical implications
  JOURNAL   N Engl J Med 321 (15), 1022-1029 (1989)
   PUBMED   2674717
  REMARK    Review article
REFERENCE   8  (bases 1 to 5641)
  AUTHORS   Ashkenazi,A., Ramachandran,J. and Capon,D.J.
  TITLE     Acetylcholine analogue stimulates DNA synthesis in brain-derived
            cells via specific muscarinic receptor subtypes
  JOURNAL   Nature 340 (6229), 146-150 (1989)
   PUBMED   2739737
REFERENCE   9  (bases 1 to 5641)
  AUTHORS   Peralta,E.G., Ashkenazi,A., Winslow,J.W., Smith,D.H.,
            Ramachandran,J. and Capon,D.J.
  TITLE     Distinct primary structures, ligand-binding properties and
            tissue-specific expression of four human muscarinic acetylcholine
            receptors
  JOURNAL   EMBO J 6 (13), 3923-3929 (1987)
   PUBMED   3443095
REFERENCE   10 (bases 1 to 5641)
  AUTHORS   Bonner,T.I., Buckley,N.J., Young,A.C. and Brann,M.R.
  TITLE     Identification of a family of muscarinic acetylcholine receptor
            genes
  JOURNAL   Science 237 (4814), 527-532 (1987)
   PUBMED   3037705
  REMARK    Erratum:[Science 1987 Sep 25;237(4822):237]
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC009264.6, AC020581.10 and
            KF458615.1.
            
            On Mar 17, 2020 this sequence version replaced NM_001006632.2.
            
            Summary: The muscarinic cholinergic receptors belong to a larger
            family of G protein-coupled receptors. The functional diversity of
            these receptors is defined by the binding of acetylcholine to these
            receptors and includes cellular responses such as adenylate cyclase
            inhibition, phosphoinositide degeneration, and potassium channel
            mediation. Muscarinic receptors influence many effects of
            acetylcholine in the central and peripheral nervous system. The
            muscarinic cholinergic receptor 2 is involved in mediation of
            bradycardia and a decrease in cardiac contractility. Multiple
            alternatively spliced transcript variants have been described for
            this gene. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY456125.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA2146982
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-89                AC009264.6         100060-100148
            90-247              AC009264.6         100236-100393
            248-4191            AC020581.10        86172-90115
            4192-4582           KF458615.1         1-391               c
            4583-5641           AC020581.10        90507-91565
FEATURES             Location/Qualifiers
     source          1..5641
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7q33"
     gene            1..5641
                     /gene="CHRM2"
                     /gene_synonym="HM2"
                     /note="cholinergic receptor muscarinic 2"
                     /db_xref="GeneID:1129"
                     /db_xref="HGNC:HGNC:1951"
                     /db_xref="MIM:118493"
     exon            1..89
                     /gene="CHRM2"
                     /gene_synonym="HM2"
                     /inference="alignment:Splign:2.1.0"
     exon            90..247
                     /gene="CHRM2"
                     /gene_synonym="HM2"
                     /inference="alignment:Splign:2.1.0"
     exon            248..5641
                     /gene="CHRM2"
                     /gene_synonym="HM2"
                     /inference="alignment:Splign:2.1.0"
     CDS             294..1694
                     /gene="CHRM2"
                     /gene_synonym="HM2"
                     /note="muscarinic M2 receptor; 7TM receptor; acetylcholine
                     receptor, muscarinic 2"
                     /codon_start=1
                     /product="muscarinic acetylcholine receptor M2"
                     /protein_id="NP_001006633.1"
                     /db_xref="CCDS:CCDS5843.1"
                     /db_xref="GeneID:1129"
                     /db_xref="HGNC:HGNC:1951"
                     /db_xref="MIM:118493"
                     /translation="MNNSTNSSNNSLALTSPYKTFEVVFIVLVAGSLSLVTIIGNILV
                     MVSIKVNRHLQTVNNYFLFSLACADLIIGVFSMNLYTLYTVIGYWPLGPVVCDLWLAL
                     DYVVSNASVMNLLIISFDRYFCVTKPLTYPVKRTTKMAGMMIAAAWVLSFILWAPAIL
                     FWQFIVGVRTVEDGECYIQFFSNAAVTFGTAIAAFYLPVIIMTVLYWHISRASKSRIK
                     KDKKEPVANQDPVSPSLVQGRIVKPNNNNMPSSDDGLEHNKIQNGKAPRDPVTENCVQ
                     GEEKESSNDSTSVSAVASNMRDDEITQDENTVSTSLGHSKDENSKQTCIRIGTKTPKS
                     DSCTPTNTTVEVVGSSGQNGDEKQNIVARKIVKMTKQPAKKKPPPSREKKVTRTILAI
                     LLAFIITWAPYNVMVLINTFCAPCIPNTVWTIGYWLCYINSTINPACYALCNATFKKT
                     FKHLLMCHYKNIGATR"
ORIGIN      
        1 acagactagc caagtggctg agacgagtgt ggggtgcgtg actctgcctg cgcgcgcgcc
       61 agccccgcag ctctcgccag agccttggga tcaagggaga aagagaaccg gcagctggcc
      121 tcggactcta agcggtgcgc agctccagcc cgagcggatc ggccctgaac ccacaaagga
      181 ctcctcgctc cttcaagcct ccaccacctc gcagccgggg aggcaactgg agcgaaacca
      241 gcgacaggtt taaatgttta tttgctactt ggctactgat tagagaacgc aaaatgaata
      301 actcaacaaa ctcctctaac aatagcctgg ctcttacaag tccttataag acatttgaag
      361 tggtgtttat tgtcctggtg gctggatccc tcagtttggt gaccattatc gggaacatcc
      421 tagtcatggt ttccattaaa gtcaaccgcc acctccagac cgtcaacaat tactttttat
      481 tcagcttggc ctgtgctgac cttatcatag gtgttttctc catgaacttg tacaccctct
      541 acactgtgat tggttactgg cctttgggac ctgtggtgtg tgacctttgg ctagccctgg
      601 actatgtggt cagcaatgcc tcagttatga atctgctcat catcagcttt gacaggtact
      661 tctgtgtcac aaaacctctg acctacccag tcaagcggac cacaaaaatg gcaggtatga
      721 tgattgcagc tgcctgggtc ctctctttca tcctctgggc tccagccatt ctcttctggc
      781 agttcattgt aggggtgaga actgtggagg atggggagtg ctacattcag tttttttcca
      841 atgctgctgt cacctttggt acggctattg cagccttcta tttgccagtg atcatcatga
      901 ctgtgctata ttggcacata tcccgagcca gcaagagcag gataaagaag gacaagaagg
      961 agcctgttgc caaccaagac cccgtttctc caagtctggt acaaggaagg atagtgaagc
     1021 caaacaataa caacatgccc agcagtgacg atggcctgga gcacaacaaa atccagaatg
     1081 gcaaagcccc cagggatcct gtgactgaaa actgtgttca gggagaggag aaggagagct
     1141 ccaatgactc cacctcagtc agtgctgttg cctctaatat gagagatgat gaaataaccc
     1201 aggatgaaaa cacagtttcc acttccctgg gccattccaa agatgagaac tctaagcaaa
     1261 catgcatcag aattggcacc aagaccccaa aaagtgactc atgtacccca actaatacca
     1321 ccgtggaggt agtggggtct tcaggtcaga atggagatga aaagcagaat attgtagccc
     1381 gcaagattgt gaagatgact aagcagcctg caaaaaagaa gcctcctcct tcccgggaaa
     1441 agaaagtcac caggacaatc ttggctattc tgttggcttt catcatcact tgggccccat
     1501 acaatgtcat ggtgctcatt aacacctttt gtgcaccttg catccccaac actgtgtgga
     1561 caattggtta ctggctttgt tacatcaaca gcactatcaa ccctgcctgc tatgcacttt
     1621 gcaatgccac cttcaagaag acctttaaac accttctcat gtgtcattat aagaacatag
     1681 gcgctacaag gtaaaatatc tttgaaaaag atagaaggtg ggcaagggga gcttgagaag
     1741 aataaaaggg ataaacgagc tcctagtttt aaaatctctg ccattgcact ttatagtctg
     1801 attacaaaac gtgcaattca ggagcccagc agtgacacac ttatcacgcc taggctccag
     1861 tttgcaaaaa ttgcacctta taaactgtca gtattaggag caatgagaca atgaaagaaa
     1921 catgttggga tcgtggattt aagaaactat acactgtttc tcataatctc ttgaagaagg
     1981 gcttctgatt ctacaatttt atcagtctct gcacaagagg aataaccttg ttcctttttt
     2041 gttacttttg ttgttgttgt tctcatgtgt ccttaagaga aggaaatgcc acagttacaa
     2101 ggtaaacatg gagacttaaa cataaagaaa taggcactat acaatgggga cataaaaaaa
     2161 gaaaatcaaa gaaggatgca gaaattgtct ccggagtgtt aagcatattt tattcttttg
     2221 ttacggtcct atttagagga ttggaatgta ataaatgctt attttttgcc tttctttttc
     2281 ccaccatgaa gagaaagcaa acaaacagaa accccaacta ggtcacacca tttttcttct
     2341 tgttatgcca ctattttgta catccctgtt ctatattgtt tatagggaaa acctacagga
     2401 ctttcactaa agctagccag agacagccat gaaaatggac gtttcacccc tcctcactgt
     2461 gtcctttgca ttcgttgggg atatcaaggt ctataaggat ttagtgcact tataatccta
     2521 tatgcgaaat gtttaaacca atctgatttt ctcctaaata gccttttata tcaacagaat
     2581 atcctaccta aaacataaac ctgtttaaga aaccatcgtc actgtaaagt tgaggtttca
     2641 tcaaattaaa taatggacta tctagttgga ccaagtttat taaagtcagg tgaattttaa
     2701 gattaaaaaa tagaagttta ggactttcta tttcaacctg aatttgcctg acccatgtat
     2761 gaaatgttaa cttatagaaa atgaactcct gactttctca gcagaaagga atgctgtttt
     2821 atttatttgg aatcctaaac aggattaaca tactcacata gcaccagtga tttctgggcc
     2881 agtccctaga gaatagaacc ttctatccaa tgcctgctaa aggatgatat tcaaattata
     2941 accaagtgag gatgtctgca attatcctat gaccccatga aaggggaaaa aacgatattt
     3001 taaaaactat tactgaaaca attacatatt ccatattcct caaattgtca attctcccta
     3061 acattattta acttcaaaat tctcttatca atagaattat agtaatcttt gtgacagaaa
     3121 tgtagtggct aactaaatag aggccaaacg agcttacaga atctgacaga aggaggtagg
     3181 tggctgttgc catgccacat ctcaccatct aattctagac cataataatc atgtcaggaa
     3241 ctattatcta agtggtggca ttatatagaa tcttgccacg tagagatttt ttatttggtc
     3301 gtctaaataa atataggatt aggattatac ggattcacat acaactagtt atttttatcc
     3361 tttatatttt tcttacagta tttgctgtag ttttccaatg agattttcta aaaatggctc
     3421 aatcaatgga aatcaaatta taaacgcatt tgcaaatttc atattttgga ttttagattt
     3481 atgatatctt gttcagtaat aataatgtta gttacatggt aaatttatca atattagacc
     3541 ccagcactta aaagaattat tttgtatgat tagcctcata gatgccacct caacattttg
     3601 gagagttaag gaggaaaaaa agcatgaggg aaaaaaaaaa ccaaaaaact tataagttag
     3661 taagaagcaa attgccagca gtatttatat ttgacttatc cttcgtaaaa acaatttcct
     3721 gtttaaataa cttcagtcat taccccccca aagtaatctt ccatatttaa agcattcatt
     3781 tatagagatt cccttaccta cagttacttc aacatgcact tttacatttt aaattcatat
     3841 ttattttcat attgtttctt caaatgaagg aatcagttga acggaaataa cgtcaagcca
     3901 gtgggctcct cgagtttctg aaaaccagag aattgactag ccaagtattt ccctttttaa
     3961 tgtattgtat tattattatg tagatttggc ttcagttggg aagaaccctt tggcatccag
     4021 gattcaggat tcagggtctt tttttgtttg ctctaataat ccaacgcacc atgaatcaag
     4081 cttttagtct ctgctgccat tgtatccttc cttttcctcc ctctgttatt gatccttgcc
     4141 acagccactg caacctcatt gaaagagaaa ctgtcacttt aaagaaagaa attgaagata
     4201 ggctgacacc atggaaacta cgtcaaacag ataagaaaaa tggaaggaat actgaaaatg
     4261 atagatgatt ggagagatat ggagattata ttcaaggatt tttattaacc actgctggat
     4321 ttgtctattt cagtggctct caaacagaga caattcccct ctccccgaaa ggggacactt
     4381 agcaatgtgt aaagacattt ttggttgcca cactgggagg aatgctattg gtatctagtg
     4441 catagaggct gggaatgctg ctaaatagcc tataatgctc aggacatttt cccccaaccc
     4501 ctttaaacaa atattatttg gctcaaaatg tcaatagttc caagattgag aaattcttgt
     4561 ctactttaag ggatttctaa agcttgcctc ttattagtgg gaaaataacc ctgcattatt
     4621 tttagaatat caatgcaaat tcctcacctg ttagaaaaag gtaatactca ccaaccatgt
     4681 atcagaaaaa cttttctcct gagtctggtg tatgtgtcag tcacagattg ggaactcagg
     4741 ccacatctac atatcaaaaa tcaccagact atgcatatcg cacatctttt ttccagcatc
     4801 ttaatgttca attatgattg ccacagatct agtcaattga ggttgcagta gttggccatc
     4861 aaaatatttg cagttgatat gatcagtttc tactggagct gcaatttttc ctttcaaaaa
     4921 atattaagag cctctttcag aaacaatatc atttatcttc tttgcattta ctggaaaggt
     4981 gtatctgtaa ggtctttgtt ttttctctcc tcatagcaat aatgtagcta tcccagagtg
     5041 atattataag aagaggagat taagaatatg gaaaaagtct gttcatttct tcgaaacaag
     5101 aaatgatttt ccttttacca gttcatcatt aatttaacgc cagctcccaa gtattagggc
     5161 agagtaatca atatgagaag ccacaacggg taagctttct gatttgaaat gtacaagctg
     5221 gatgtccaac atactcctgc tctttgttca tttgcttttt tgatcctgaa gatctgacat
     5281 tgggagagat gggaattcaa aatgatggca acaattactc agcaatgcag tctgttttta
     5341 ttttctccta cttagttcat tgacagtttc ctcctcgccc cagtttgata atatcccttt
     5401 caacaaccat agtaccggtg ttgcaaatag tttaggaaag cacccaaagc tggatgaaat
     5461 tgtgcaaagt gtattctcag catgtatatt caataccatg cctcttaaaa atgcttctct
     5521 taaagggtgc acgctgtagc atgaaatgtg tgcatattta atgtatcttc ctggaatatg
     5581 ctatgtgcta aatatatata tattctataa ataaaataat aaaatgtaca atagaaatac
     5641 a
//
