LOCUS       NM_001006630            5971 bp    mRNA    linear   PRI 10-JUN-2024
DEFINITION  Homo sapiens cholinergic receptor muscarinic 2 (CHRM2), transcript
            variant 1, mRNA.
ACCESSION   NM_001006630
VERSION     NM_001006630.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5971)
  AUTHORS   Fasciani,I., Petragnano,F., Wang,Z., Edwards,R., Telugu,N.,
            Pietrantoni,I., Zabel,U., Zauber,H., Grieben,M., Terzenidou,M.E.,
            Di Gregorio,J., Pellegrini,C., Santini,S. Jr., Taddei,A.R.,
            Pohl,B., Aringhieri,S., Carli,M., Aloisi,G., Marampon,F.,
            Charlesworth,E., Roman,A., Diecke,S., Flati,V., Giorgi,F.,
            Amicarelli,F., Tobin,A.B., Scarselli,M., Tokatlidis,K., Rossi,M.,
            Lohse,M.J., Annibale,P. and Maggio,R.
  TITLE     The C-terminus of the prototypical M2 muscarinic receptor localizes
            to the mitochondria and regulates cell respiration under stress
            conditions
  JOURNAL   PLoS Biol 22 (4), e3002582 (2024)
   PUBMED   38683874
  REMARK    GeneRIF: The C-terminus of the prototypical M2 muscarinic receptor
            localizes to the mitochondria and regulates cell respiration under
            stress conditions.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5971)
  AUTHORS   Guerriero,C., Fanfarillo,R., Mancini,P., Sterbini,V.,
            Guarguaglini,G., Sforna,L., Michelucci,A., Catacuzzeno,L. and
            Tata,A.M.
  TITLE     M2 muscarinic receptors negatively modulate cell migration in human
            glioblastoma cells
  JOURNAL   Neurochem Int 174, 105673 (2024)
   PUBMED   38185384
  REMARK    GeneRIF: M2 muscarinic receptors negatively modulate cell migration
            in human glioblastoma cells.
REFERENCE   3  (bases 1 to 5971)
  AUTHORS   Refisch,A., Komatsuzaki,S., Ungelenk,M., Chung,H.Y., Schumann,A.,
            Schilling,S.S., Jantzen,W., Schroder,S., Muhleisen,T.W.,
            Nothen,M.M., Hubner,C.A. and Bar,K.J.
  TITLE     Associations of common genetic risk variants of the muscarinic
            acetylcholine receptor M2 with cardiac autonomic dysfunction in
            patients with schizophrenia
  JOURNAL   World J Biol Psychiatry 24 (1), 1-11 (2023)
   PUBMED   35172679
REFERENCE   4  (bases 1 to 5971)
  AUTHORS   Kim,M., Hong,S., Yankeelov,T.E., Yeh,H.C. and Liu,Y.L.
  TITLE     Deep learning-based classification of breast cancer cells using
            transmembrane receptor dynamics
  JOURNAL   Bioinformatics 38 (1), 243-249 (2021)
   PUBMED   34390568
  REMARK    GeneRIF: Deep learning-based classification of breast cancer cells
            using transmembrane receptor dynamics.
REFERENCE   5  (bases 1 to 5971)
  AUTHORS   Katayama,K., Suzuki,K., Suno,R., Kise,R., Tsujimoto,H., Iwata,S.,
            Inoue,A., Kobayashi,T. and Kandori,H.
  TITLE     Vibrational spectroscopy analysis of ligand efficacy in human M2
            muscarinic acetylcholine receptor (M2R)
  JOURNAL   Commun Biol 4 (1), 1321 (2021)
   PUBMED   34815515
  REMARK    GeneRIF: Vibrational spectroscopy analysis of ligand efficacy in
            human M2 muscarinic acetylcholine receptor (M2R).
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 5971)
  AUTHORS   van Koppen,C.J. and Nathanson,N.M.
  TITLE     Site-directed mutagenesis of the m2 muscarinic acetylcholine
            receptor. Analysis of the role of N-glycosylation in receptor
            expression and function
  JOURNAL   J Biol Chem 265 (34), 20887-20892 (1990)
   PUBMED   2249995
REFERENCE   7  (bases 1 to 5971)
  AUTHORS   Goyal,R.K.
  TITLE     Muscarinic receptor subtypes. Physiology and clinical implications
  JOURNAL   N Engl J Med 321 (15), 1022-1029 (1989)
   PUBMED   2674717
  REMARK    Review article
REFERENCE   8  (bases 1 to 5971)
  AUTHORS   Ashkenazi,A., Ramachandran,J. and Capon,D.J.
  TITLE     Acetylcholine analogue stimulates DNA synthesis in brain-derived
            cells via specific muscarinic receptor subtypes
  JOURNAL   Nature 340 (6229), 146-150 (1989)
   PUBMED   2739737
REFERENCE   9  (bases 1 to 5971)
  AUTHORS   Peralta,E.G., Ashkenazi,A., Winslow,J.W., Smith,D.H.,
            Ramachandran,J. and Capon,D.J.
  TITLE     Distinct primary structures, ligand-binding properties and
            tissue-specific expression of four human muscarinic acetylcholine
            receptors
  JOURNAL   EMBO J 6 (13), 3923-3929 (1987)
   PUBMED   3443095
REFERENCE   10 (bases 1 to 5971)
  AUTHORS   Bonner,T.I., Buckley,N.J., Young,A.C. and Brann,M.R.
  TITLE     Identification of a family of muscarinic acetylcholine receptor
            genes
  JOURNAL   Science 237 (4814), 527-532 (1987)
   PUBMED   3037705
  REMARK    Erratum:[Science 1987 Sep 25;237(4822):237]
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC009264.6, AC020581.10 and
            KF458615.1.
            
            On Mar 17, 2020 this sequence version replaced NM_001006630.1.
            
            Summary: The muscarinic cholinergic receptors belong to a larger
            family of G protein-coupled receptors. The functional diversity of
            these receptors is defined by the binding of acetylcholine to these
            receptors and includes cellular responses such as adenylate cyclase
            inhibition, phosphoinositide degeneration, and potassium channel
            mediation. Muscarinic receptors influence many effects of
            acetylcholine in the central and peripheral nervous system. The
            muscarinic cholinergic receptor 2 is involved in mediation of
            bradycardia and a decrease in cardiac contractility. Multiple
            alternatively spliced transcript variants have been described for
            this gene. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803616.34441.1, AY034603.2
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2144335, SAMEA2146982
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000680005.1/ ENSP00000505686.1
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-341               AC009264.6         99627-99967
            342-499             AC009264.6         100236-100393
            500-577             AC020581.10        63539-63616
            578-4521            AC020581.10        86172-90115
            4522-4912           KF458615.1         1-391               c
            4913-5971           AC020581.10        90507-91565
FEATURES             Location/Qualifiers
     source          1..5971
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7q33"
     gene            1..5971
                     /gene="CHRM2"
                     /gene_synonym="HM2"
                     /note="cholinergic receptor muscarinic 2"
                     /db_xref="GeneID:1129"
                     /db_xref="HGNC:HGNC:1951"
                     /db_xref="MIM:118493"
     exon            1..341
                     /gene="CHRM2"
                     /gene_synonym="HM2"
                     /inference="alignment:Splign:2.1.0"
     exon            342..499
                     /gene="CHRM2"
                     /gene_synonym="HM2"
                     /inference="alignment:Splign:2.1.0"
     exon            500..577
                     /gene="CHRM2"
                     /gene_synonym="HM2"
                     /inference="alignment:Splign:2.1.0"
     exon            578..5971
                     /gene="CHRM2"
                     /gene_synonym="HM2"
                     /inference="alignment:Splign:2.1.0"
     CDS             624..2024
                     /gene="CHRM2"
                     /gene_synonym="HM2"
                     /note="muscarinic M2 receptor; 7TM receptor; acetylcholine
                     receptor, muscarinic 2"
                     /codon_start=1
                     /product="muscarinic acetylcholine receptor M2"
                     /protein_id="NP_001006631.1"
                     /db_xref="CCDS:CCDS5843.1"
                     /db_xref="GeneID:1129"
                     /db_xref="HGNC:HGNC:1951"
                     /db_xref="MIM:118493"
                     /translation="MNNSTNSSNNSLALTSPYKTFEVVFIVLVAGSLSLVTIIGNILV
                     MVSIKVNRHLQTVNNYFLFSLACADLIIGVFSMNLYTLYTVIGYWPLGPVVCDLWLAL
                     DYVVSNASVMNLLIISFDRYFCVTKPLTYPVKRTTKMAGMMIAAAWVLSFILWAPAIL
                     FWQFIVGVRTVEDGECYIQFFSNAAVTFGTAIAAFYLPVIIMTVLYWHISRASKSRIK
                     KDKKEPVANQDPVSPSLVQGRIVKPNNNNMPSSDDGLEHNKIQNGKAPRDPVTENCVQ
                     GEEKESSNDSTSVSAVASNMRDDEITQDENTVSTSLGHSKDENSKQTCIRIGTKTPKS
                     DSCTPTNTTVEVVGSSGQNGDEKQNIVARKIVKMTKQPAKKKPPPSREKKVTRTILAI
                     LLAFIITWAPYNVMVLINTFCAPCIPNTVWTIGYWLCYINSTINPACYALCNATFKKT
                     FKHLLMCHYKNIGATR"
ORIGIN      
        1 attgcagcta atcgaacaca cacgcacgaa tacagacacg ctctcctgct gtactaaagg
       61 cgccagggcg caaagaccta gggagcgcgc gcgggcacac acacacacac acacacacac
      121 acagacacac acacactcac acactccagg ctgcgggttg gccagagcct ggaaatcgca
      181 gatttcacca ggaaatcgcc gaggtacccg aataggtgtt gcctcccaaa actaattcct
      241 acttccccaa gcactttggt gaagaaatga atccagccca gctcgccgag gcatccaggt
      301 ctccattcta ttttggtctc caggctcttc gtgaaagcca gatcaaggga gaaagagaac
      361 cggcagctgg cctcggactc taagcggtgc gcagctccag cccgagcgga tcggccctga
      421 acccacaaag gactcctcgc tccttcaagc ctccaccacc tcgcagccgg ggaggcaact
      481 ggagcgaaac cagcgacagg ttgttagcga catgagtaca gggtctggat cttacccatt
      541 caagagtgta ttctcagcac ccaatgcctg gcatatagtt taaatgttta tttgctactt
      601 ggctactgat tagagaacgc aaaatgaata actcaacaaa ctcctctaac aatagcctgg
      661 ctcttacaag tccttataag acatttgaag tggtgtttat tgtcctggtg gctggatccc
      721 tcagtttggt gaccattatc gggaacatcc tagtcatggt ttccattaaa gtcaaccgcc
      781 acctccagac cgtcaacaat tactttttat tcagcttggc ctgtgctgac cttatcatag
      841 gtgttttctc catgaacttg tacaccctct acactgtgat tggttactgg cctttgggac
      901 ctgtggtgtg tgacctttgg ctagccctgg actatgtggt cagcaatgcc tcagttatga
      961 atctgctcat catcagcttt gacaggtact tctgtgtcac aaaacctctg acctacccag
     1021 tcaagcggac cacaaaaatg gcaggtatga tgattgcagc tgcctgggtc ctctctttca
     1081 tcctctgggc tccagccatt ctcttctggc agttcattgt aggggtgaga actgtggagg
     1141 atggggagtg ctacattcag tttttttcca atgctgctgt cacctttggt acggctattg
     1201 cagccttcta tttgccagtg atcatcatga ctgtgctata ttggcacata tcccgagcca
     1261 gcaagagcag gataaagaag gacaagaagg agcctgttgc caaccaagac cccgtttctc
     1321 caagtctggt acaaggaagg atagtgaagc caaacaataa caacatgccc agcagtgacg
     1381 atggcctgga gcacaacaaa atccagaatg gcaaagcccc cagggatcct gtgactgaaa
     1441 actgtgttca gggagaggag aaggagagct ccaatgactc cacctcagtc agtgctgttg
     1501 cctctaatat gagagatgat gaaataaccc aggatgaaaa cacagtttcc acttccctgg
     1561 gccattccaa agatgagaac tctaagcaaa catgcatcag aattggcacc aagaccccaa
     1621 aaagtgactc atgtacccca actaatacca ccgtggaggt agtggggtct tcaggtcaga
     1681 atggagatga aaagcagaat attgtagccc gcaagattgt gaagatgact aagcagcctg
     1741 caaaaaagaa gcctcctcct tcccgggaaa agaaagtcac caggacaatc ttggctattc
     1801 tgttggcttt catcatcact tgggccccat acaatgtcat ggtgctcatt aacacctttt
     1861 gtgcaccttg catccccaac actgtgtgga caattggtta ctggctttgt tacatcaaca
     1921 gcactatcaa ccctgcctgc tatgcacttt gcaatgccac cttcaagaag acctttaaac
     1981 accttctcat gtgtcattat aagaacatag gcgctacaag gtaaaatatc tttgaaaaag
     2041 atagaaggtg ggcaagggga gcttgagaag aataaaaggg ataaacgagc tcctagtttt
     2101 aaaatctctg ccattgcact ttatagtctg attacaaaac gtgcaattca ggagcccagc
     2161 agtgacacac ttatcacgcc taggctccag tttgcaaaaa ttgcacctta taaactgtca
     2221 gtattaggag caatgagaca atgaaagaaa catgttggga tcgtggattt aagaaactat
     2281 acactgtttc tcataatctc ttgaagaagg gcttctgatt ctacaatttt atcagtctct
     2341 gcacaagagg aataaccttg ttcctttttt gttacttttg ttgttgttgt tctcatgtgt
     2401 ccttaagaga aggaaatgcc acagttacaa ggtaaacatg gagacttaaa cataaagaaa
     2461 taggcactat acaatgggga cataaaaaaa gaaaatcaaa gaaggatgca gaaattgtct
     2521 ccggagtgtt aagcatattt tattcttttg ttacggtcct atttagagga ttggaatgta
     2581 ataaatgctt attttttgcc tttctttttc ccaccatgaa gagaaagcaa acaaacagaa
     2641 accccaacta ggtcacacca tttttcttct tgttatgcca ctattttgta catccctgtt
     2701 ctatattgtt tatagggaaa acctacagga ctttcactaa agctagccag agacagccat
     2761 gaaaatggac gtttcacccc tcctcactgt gtcctttgca ttcgttgggg atatcaaggt
     2821 ctataaggat ttagtgcact tataatccta tatgcgaaat gtttaaacca atctgatttt
     2881 ctcctaaata gccttttata tcaacagaat atcctaccta aaacataaac ctgtttaaga
     2941 aaccatcgtc actgtaaagt tgaggtttca tcaaattaaa taatggacta tctagttgga
     3001 ccaagtttat taaagtcagg tgaattttaa gattaaaaaa tagaagttta ggactttcta
     3061 tttcaacctg aatttgcctg acccatgtat gaaatgttaa cttatagaaa atgaactcct
     3121 gactttctca gcagaaagga atgctgtttt atttatttgg aatcctaaac aggattaaca
     3181 tactcacata gcaccagtga tttctgggcc agtccctaga gaatagaacc ttctatccaa
     3241 tgcctgctaa aggatgatat tcaaattata accaagtgag gatgtctgca attatcctat
     3301 gaccccatga aaggggaaaa aacgatattt taaaaactat tactgaaaca attacatatt
     3361 ccatattcct caaattgtca attctcccta acattattta acttcaaaat tctcttatca
     3421 atagaattat agtaatcttt gtgacagaaa tgtagtggct aactaaatag aggccaaacg
     3481 agcttacaga atctgacaga aggaggtagg tggctgttgc catgccacat ctcaccatct
     3541 aattctagac cataataatc atgtcaggaa ctattatcta agtggtggca ttatatagaa
     3601 tcttgccacg tagagatttt ttatttggtc gtctaaataa atataggatt aggattatac
     3661 ggattcacat acaactagtt atttttatcc tttatatttt tcttacagta tttgctgtag
     3721 ttttccaatg agattttcta aaaatggctc aatcaatgga aatcaaatta taaacgcatt
     3781 tgcaaatttc atattttgga ttttagattt atgatatctt gttcagtaat aataatgtta
     3841 gttacatggt aaatttatca atattagacc ccagcactta aaagaattat tttgtatgat
     3901 tagcctcata gatgccacct caacattttg gagagttaag gaggaaaaaa agcatgaggg
     3961 aaaaaaaaaa ccaaaaaact tataagttag taagaagcaa attgccagca gtatttatat
     4021 ttgacttatc cttcgtaaaa acaatttcct gtttaaataa cttcagtcat taccccccca
     4081 aagtaatctt ccatatttaa agcattcatt tatagagatt cccttaccta cagttacttc
     4141 aacatgcact tttacatttt aaattcatat ttattttcat attgtttctt caaatgaagg
     4201 aatcagttga acggaaataa cgtcaagcca gtgggctcct cgagtttctg aaaaccagag
     4261 aattgactag ccaagtattt ccctttttaa tgtattgtat tattattatg tagatttggc
     4321 ttcagttggg aagaaccctt tggcatccag gattcaggat tcagggtctt tttttgtttg
     4381 ctctaataat ccaacgcacc atgaatcaag cttttagtct ctgctgccat tgtatccttc
     4441 cttttcctcc ctctgttatt gatccttgcc acagccactg caacctcatt gaaagagaaa
     4501 ctgtcacttt aaagaaagaa attgaagata ggctgacacc atggaaacta cgtcaaacag
     4561 ataagaaaaa tggaaggaat actgaaaatg atagatgatt ggagagatat ggagattata
     4621 ttcaaggatt tttattaacc actgctggat ttgtctattt cagtggctct caaacagaga
     4681 caattcccct ctccccgaaa ggggacactt agcaatgtgt aaagacattt ttggttgcca
     4741 cactgggagg aatgctattg gtatctagtg catagaggct gggaatgctg ctaaatagcc
     4801 tataatgctc aggacatttt cccccaaccc ctttaaacaa atattatttg gctcaaaatg
     4861 tcaatagttc caagattgag aaattcttgt ctactttaag ggatttctaa agcttgcctc
     4921 ttattagtgg gaaaataacc ctgcattatt tttagaatat caatgcaaat tcctcacctg
     4981 ttagaaaaag gtaatactca ccaaccatgt atcagaaaaa cttttctcct gagtctggtg
     5041 tatgtgtcag tcacagattg ggaactcagg ccacatctac atatcaaaaa tcaccagact
     5101 atgcatatcg cacatctttt ttccagcatc ttaatgttca attatgattg ccacagatct
     5161 agtcaattga ggttgcagta gttggccatc aaaatatttg cagttgatat gatcagtttc
     5221 tactggagct gcaatttttc ctttcaaaaa atattaagag cctctttcag aaacaatatc
     5281 atttatcttc tttgcattta ctggaaaggt gtatctgtaa ggtctttgtt ttttctctcc
     5341 tcatagcaat aatgtagcta tcccagagtg atattataag aagaggagat taagaatatg
     5401 gaaaaagtct gttcatttct tcgaaacaag aaatgatttt ccttttacca gttcatcatt
     5461 aatttaacgc cagctcccaa gtattagggc agagtaatca atatgagaag ccacaacggg
     5521 taagctttct gatttgaaat gtacaagctg gatgtccaac atactcctgc tctttgttca
     5581 tttgcttttt tgatcctgaa gatctgacat tgggagagat gggaattcaa aatgatggca
     5641 acaattactc agcaatgcag tctgttttta ttttctccta cttagttcat tgacagtttc
     5701 ctcctcgccc cagtttgata atatcccttt caacaaccat agtaccggtg ttgcaaatag
     5761 tttaggaaag cacccaaagc tggatgaaat tgtgcaaagt gtattctcag catgtatatt
     5821 caataccatg cctcttaaaa atgcttctct taaagggtgc acgctgtagc atgaaatgtg
     5881 tgcatattta atgtatcttc ctggaatatg ctatgtgcta aatatatata tattctataa
     5941 ataaaataat aaaatgtaca atagaaatac a
//
