LOCUS       NM_001304415            1564 bp    mRNA    linear   PRI 22-SEP-2024
DEFINITION  Homo sapiens chordin like 2 (CHRDL2), transcript variant 5, mRNA.
ACCESSION   NM_001304415
VERSION     NM_001304415.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Wang,L., Xu,W., Mei,Y., Wang,X., Liu,W., Zhu,Z. and Ni,Z.
  TITLE     CHRDL2 promotes cell proliferation by activating the YAP/TAZ
            signaling pathway in gastric cancer
  JOURNAL   Free Radic Biol Med 193 (Pt 1), 158-170 (2022)
   PUBMED   36206931
  REMARK    GeneRIF: CHRDL2 promotes cell proliferation by activating the
            YAP/TAZ signaling pathway in gastric cancer.
REFERENCE   2  (bases 1 to 1564)
  AUTHORS   Chen,H., Pan,R., Li,H., Zhang,W., Ren,C., Lu,Q., Chen,H., Zhang,X.
            and Nie,Y.
  TITLE     CHRDL2 promotes osteosarcoma cell proliferation and metastasis
            through the BMP-9/PI3K/AKT pathway
  JOURNAL   Cell Biol Int 45 (3), 623-632 (2021)
   PUBMED   33245175
  REMARK    GeneRIF: CHRDL2 promotes osteosarcoma cell proliferation and
            metastasis through the BMP-9/PI3K/AKT pathway.
REFERENCE   3  (bases 1 to 1564)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 1564)
  AUTHORS   Bunyavanich,S., Schadt,E.E., Himes,B.E., Lasky-Su,J., Qiu,W.,
            Lazarus,R., Ziniti,J.P., Cohain,A., Linderman,M., Torgerson,D.G.,
            Eng,C.S., Pino-Yanes,M., Padhukasahasram,B., Yang,J.J.,
            Mathias,R.A., Beaty,T.H., Li,X., Graves,P., Romieu,I., Navarro
            Bdel,R., Salam,M.T., Vora,H., Nicolae,D.L., Ober,C., Martinez,F.D.,
            Bleecker,E.R., Meyers,D.A., Gauderman,W.J., Gilliland,F.,
            Burchard,E.G., Barnes,K.C., Williams,L.K., London,S.J., Zhang,B.,
            Raby,B.A. and Weiss,S.T.
  TITLE     Integrated genome-wide association, coexpression network, and
            expression single nucleotide polymorphism analysis identifies novel
            pathway in allergic rhinitis
  JOURNAL   BMC Med Genomics 7, 48 (2014)
   PUBMED   25085501
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1564)
  AUTHORS   Vasiljevic,A., Champier,J., Figarella-Branger,D., Wierinckx,A.,
            Jouvet,A. and Fevre-Montange,M.
  TITLE     Molecular characterization of central neurocytomas: potential
            markers for tumor typing and progression
  JOURNAL   Neuropathology 33 (2), 149-161 (2013)
   PUBMED   22816789
REFERENCE   6  (bases 1 to 1564)
  AUTHORS   Yerges,L.M., Klei,L., Cauley,J.A., Roeder,K., Kammerer,C.M.,
            Moffett,S.P., Ensrud,K.E., Nestlerode,C.S., Marshall,L.M.,
            Hoffman,A.R., Lewis,C., Lang,T.F., Barrett-Connor,E., Ferrell,R.E.,
            Orwoll,E.S. and Zmuda,J.M.
  CONSRTM   MrOS Research Group
  TITLE     High-density association study of 383 candidate genes for
            volumetric BMD at the femoral neck and lumbar spine among older men
  JOURNAL   J Bone Miner Res 24 (12), 2039-2049 (2009)
   PUBMED   19453261
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   7  (bases 1 to 1564)
  AUTHORS   Zhang,Z. and Henzel,W.J.
  TITLE     Signal peptide prediction based on analysis of experimentally
            verified cleavage sites
  JOURNAL   Protein Sci 13 (10), 2819-2824 (2004)
   PUBMED   15340161
REFERENCE   8  (bases 1 to 1564)
  AUTHORS   Oren,A., Toporik,A., Biton,S., Almogy,N., Eshel,D., Bernstein,J.,
            Savitsky,K. and Rotman,G.
  TITLE     hCHL2, a novel chordin-related gene, displays differential
            expression and complex alternative splicing in human tissues and
            during myoblast and osteoblast maturation
  JOURNAL   Gene 331, 17-31 (2004)
   PUBMED   15094188
  REMARK    GeneRIF: Differential expression of CHL2 variants was observed
            during myoblast and osteoblast differentiation, implying a role for
            this gene in these physiological processes.
REFERENCE   9  (bases 1 to 1564)
  AUTHORS   Nakayama,N., Han,C.Y., Cam,L., Lee,J.I., Pretorius,J., Fisher,S.,
            Rosenfeld,R., Scully,S., Nishinakamura,R., Duryea,D., Van,G.,
            Bolon,B., Yokota,T. and Zhang,K.
  TITLE     A novel chordin-like BMP inhibitor, CHL2, expressed preferentially
            in chondrocytes of developing cartilage and osteoarthritic joint
            cartilage
  JOURNAL   Development 131 (1), 229-240 (2004)
   PUBMED   14660436
  REMARK    GeneRIF: CHL2 expression is restricted to chondrocytes of various
            developing joint cartilage surfaces and connective tissues in
            reproductive organs
REFERENCE   10 (bases 1 to 1564)
  AUTHORS   Wu,I. and Moses,M.A.
  TITLE     BNF-1, a novel gene encoding a putative extracellular matrix
            protein, is overexpressed in tumor tissues
  JOURNAL   Gene 311, 105-110 (2003)
   PUBMED   12853144
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AY279092.1, AY358522.1 and
            AP001324.4.
            
            On Aug 14, 2020 this sequence version replaced NM_001304415.1.
            
            Summary: This gene encodes a member of the chordin family of
            proteins. Chordin family members are secreted proteins that share a
            cysteine-rich pro-collagen repeat domain and associate with members
            of the transforming growth factor beta superfamily. In vitro assays
            demonstrate a direct interaction between the encoded protein and
            human activin A. This gene is expressed in many tissues including
            osteoblasts, where it is differentially expressed during
            differentiation. In addition, its expression is upregulated in
            human osteoarthritic joint cartilage, suggesting a role in adult
            cartilage regeneration. [provided by RefSeq, Jan 2015].
            
            Transcript Variant: This variant (5) has a distinct 5' UTR,
            initiates translation at an alternate codon, and has multiple
            coding region differences compared to variant 1. The encoded
            isoform (5) has distinct N- and C-termini and is shorter than
            isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: mixed sample support SAMEA1965299, SAMEA1966682
                              [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-582               AY279092.1         1-582
            583-883             AY358522.1         480-780
            884-884             AP001324.4         87465-87465         c
            885-904             AY358522.1         782-801
            905-905             AP001324.4         87444-87444         c
            906-1140            AY358522.1         803-1037
            1141-1314           AY358522.1         1233-1406
            1315-1564           AY358522.1         1462-1711
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11q13.4"
     gene            1..1564
                     /gene="CHRDL2"
                     /gene_synonym="BNF1; CHL2"
                     /note="chordin like 2"
                     /db_xref="GeneID:25884"
                     /db_xref="HGNC:HGNC:24168"
                     /db_xref="MIM:613127"
     exon            1..584
                     /gene="CHRDL2"
                     /gene_synonym="BNF1; CHL2"
                     /inference="alignment:Splign:2.1.0"
     CDS             450..1484
                     /gene="CHRDL2"
                     /gene_synonym="BNF1; CHL2"
                     /note="isoform 5 is encoded by transcript variant 5;
                     breast tumor novel factor 1; chordin-like protein 2;
                     chordin-related protein 2"
                     /codon_start=1
                     /product="chordin-like protein 2 isoform 5"
                     /protein_id="NP_001291344.1"
                     /db_xref="GeneID:25884"
                     /db_xref="HGNC:HGNC:24168"
                     /db_xref="MIM:613127"
                     /translation="MALVGLPGPDMFCLFHGKRYSPGESWHPYLEPQGLMYCLRCTCS
                     EGAHVSCYRLHCPPVHCPQPVTEPQQCCPKCVEPHTPSGLRAPPKSCQHNGTMYQHGE
                     IFSAHELFPSRLPNQCVLCSCTEGQIYCGLTTCPEPGCPAPLPLPDSCCQACKDEASE
                     QSDEEDSVQSLHGVRHPQDPCSSDAGRKRGPGTPAPTGLSAPLSFIPRHFRPKGAGST
                     TVKIVLKEKHKKEDKADPGHSEISSTRCPKAPGRVLVHTSVSPSPDNLRRFALEHEAS
                     DLVEIYLWKLVKGIFHLTQIKKVRKQDFQKEAQHFRLLAGPHEGHWNVFLAQTLELKV
                     TASPDKVTKT"
     exon            585..678
                     /gene="CHRDL2"
                     /gene_synonym="BNF1; CHL2"
                     /inference="alignment:Splign:2.1.0"
     exon            679..821
                     /gene="CHRDL2"
                     /gene_synonym="BNF1; CHL2"
                     /inference="alignment:Splign:2.1.0"
     exon            822..915
                     /gene="CHRDL2"
                     /gene_synonym="BNF1; CHL2"
                     /inference="alignment:Splign:2.1.0"
     exon            916..971
                     /gene="CHRDL2"
                     /gene_synonym="BNF1; CHL2"
                     /inference="alignment:Splign:2.1.0"
     exon            972..1140
                     /gene="CHRDL2"
                     /gene_synonym="BNF1; CHL2"
                     /inference="alignment:Splign:2.1.0"
     exon            1141..1314
                     /gene="CHRDL2"
                     /gene_synonym="BNF1; CHL2"
                     /inference="alignment:Splign:2.1.0"
     exon            1315..1407
                     /gene="CHRDL2"
                     /gene_synonym="BNF1; CHL2"
                     /inference="alignment:Splign:2.1.0"
     exon            1408..1564
                     /gene="CHRDL2"
                     /gene_synonym="BNF1; CHL2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1542..1547
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CHRDL2"
                     /gene_synonym="BNF1; CHL2"
                     /note="hexamer: AATAAG"
     polyA_site      1564
                     /gene="CHRDL2"
                     /gene_synonym="BNF1; CHL2"
                     /note="major polyA site"
ORIGIN      
        1 ggacaaataa aaaggaaaca agcatgattg tgagggcaga ggagcgtggg actgagtcag
       61 gagactggtg ctgtcatcgc tgcctggtga ctgacttgct gtgtggccct caggtgtaac
      121 ttaccctctc tgggcctcat ttgtctaatc ataataatta acgctgatac catgatataa
      181 atctgtacag catttcactg cttgattccc taactgccct gtgagataag cgttaaggct
      241 cagagacagt ggcatgccca gtgttgcaca gtaagtgtgt ggtaaagccg agattcaaac
      301 tcagaccttc tggccccttg cctaggagag catgcccagt tgtctagcag attctctttt
      361 gcctgagtgg cccagatgac atctctttta gagctagaaa gaaggagaaa tgagacaggg
      421 tctttgggct ggagcctcct gggactaaca tggcactggt cggtttgcca ggcccagaca
      481 tgttctgcct tttccatggg aagagatact cccccggcga gagctggcac ccctacttgg
      541 agccacaagg cctgatgtac tgcctgcgct gtacctgctc agagggcgcc catgtgagtt
      601 gttaccgcct ccactgtccg cctgtccact gcccccagcc tgtgacggag ccacagcaat
      661 gctgtcccaa gtgtgtggaa cctcacactc cctctggact ccgggcccca ccaaagtcct
      721 gccagcacaa cgggaccatg taccaacacg gagagatctt cagtgcccat gagctgttcc
      781 cctcccgcct gcccaaccag tgtgtcctct gcagctgcac agagggccag atctactgcg
      841 gcctcacaac ctgccccgaa ccaggctgcc cagcacccct cccgctgcca gactcctgct
      901 gccaggcctg caaagatgag gcaagtgagc aatcggatga agaggacagt gtgcagtcgc
      961 tccatggggt gagacatcct caggatccat gttccagtga tgctgggaga aagagaggcc
     1021 cgggcacccc agcccccact ggcctcagcg cccctctgag cttcatccct cgccacttca
     1081 gacccaaggg agcaggcagc acaactgtca agatcgtcct gaaggagaaa cataagaaag
     1141 aggacaaagc agaccctggc cacagtgaga tcagttctac caggtgtccc aaggcaccgg
     1201 gccgggtcct cgtccacaca tcggtatccc caagcccaga caacctgcgt cgctttgccc
     1261 tggaacacga ggcctcggac ttggtggaga tctacctctg gaagctggta aaaggaatct
     1321 tccacttgac tcagatcaag aaagtcagga agcaagactt ccagaaagag gcacagcact
     1381 tccgactgct cgctggcccc cacgaaggtc actggaacgt cttcctagcc cagaccctgg
     1441 agctgaaggt cacggccagt ccagacaaag tgaccaagac ataacaaaga cctaacagtt
     1501 gcagatatga gctgtataat tgttgttatt atatattaat aaataagaag ttgcattacc
     1561 ctca
//
