LOCUS       NM_020244               1572 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens choline phosphotransferase 1 (CHPT1), mRNA.
ACCESSION   NM_020244
VERSION     NM_020244.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Wen,S., He,Y., Wang,L., Zhang,J., Quan,C., Niu,Y. and Huang,H.
  TITLE     Aberrant activation of super enhancer and choline metabolism drive
            antiandrogen therapy resistance in prostate cancer
  JOURNAL   Oncogene 39 (42), 6556-6571 (2020)
   PUBMED   32917955
  REMARK    GeneRIF: Aberrant activation of super enhancer and choline
            metabolism drive antiandrogen therapy resistance in prostate
            cancer.
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   Shen,W., Kuang,P., Wang,B., Zeng,Q., Chen,C. and Lin,X.
  TITLE     Genetic Polymorphisms of LPCAT1, CHPT1 and PCYT1B and Risk of
            Neonatal Respiratory Distress Syndrome among a Chinese Han
            Population
  JOURNAL   Pediatr Neonatol 61 (3), 318-324 (2020)
   PUBMED   31964590
  REMARK    GeneRIF: Genetic Polymorphisms of LPCAT1, CHPT1 and PCYT1B and Risk
            of Neonatal Respiratory Distress Syndrome among a Chinese Han
            Population.
REFERENCE   3  (bases 1 to 1572)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 1572)
  AUTHORS   Detopoulou,P., Fragopoulou,E., Nomikos,T., Yannakoulia,M.,
            Stamatakis,G., Panagiotakos,D.B. and Antonopoulou,S.
  TITLE     The relation of diet with PAF and its metabolic enzymes in healthy
            volunteers
  JOURNAL   Eur J Nutr 54 (1), 25-34 (2015)
   PUBMED   24639073
  REMARK    GeneRIF: Data suggest that PAF-CPT level in blood can be altered by
            diet; up-regulation of PAF-CPT is associated with diets high in fat
            and cholesterol; down-regulation of PAF-CPT is associated with
            healthy diet patterns of the population studied in Greece.
REFERENCE   5  (bases 1 to 1572)
  AUTHORS   Akech,J., Sinha Roy,S. and Das,S.K.
  TITLE     Modulation of cholinephosphotransferase activity in breast cancer
            cell lines by Ro5-4864, a peripheral benzodiazepine receptor
            agonist
  JOURNAL   Biochem Biophys Res Commun 333 (1), 35-41 (2005)
   PUBMED   15936720
  REMARK    GeneRIF: Furthermore, the activity of CPT in forming PC is
            increased in the breast cancer cell lines cultured for 24 h.
REFERENCE   6  (bases 1 to 1572)
  AUTHORS   Ghosh,A., Akech,J., Mukherjee,S. and Das,S.K.
  TITLE     Differential expression of cholinephosphotransferase in normal and
            cancerous human mammary epithelial cells
  JOURNAL   Biochem Biophys Res Commun 297 (4), 1043-1048 (2002)
   PUBMED   12359261
REFERENCE   7  (bases 1 to 1572)
  AUTHORS   Henneberry,A.L., Wright,M.M. and McMaster,C.R.
  TITLE     The major sites of cellular phospholipid synthesis and molecular
            determinants of Fatty Acid and lipid head group specificity
  JOURNAL   Mol Biol Cell 13 (9), 3148-3161 (2002)
   PUBMED   12221122
REFERENCE   8  (bases 1 to 1572)
  AUTHORS   Henneberry,A.L., Wistow,G. and McMaster,C.R.
  TITLE     Cloning, genomic organization, and characterization of a human
            cholinephosphotransferase
  JOURNAL   J Biol Chem 275 (38), 29808-29815 (2000)
   PUBMED   10893425
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CD300296.1, BC050429.1 and AC063950.37.
            
            On Nov 22, 2018 this sequence version replaced NM_020244.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC050429.1, SRR1163657.171826.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000229266.8/ ENSP00000229266.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-8                 CD300296.1         28-35
            9-1566              BC050429.1         3-1560
            1567-1572           AC063950.37        8695-8700
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q23.2"
     gene            1..1572
                     /gene="CHPT1"
                     /gene_synonym="CPT; CPT1"
                     /note="choline phosphotransferase 1"
                     /db_xref="GeneID:56994"
                     /db_xref="HGNC:HGNC:17852"
                     /db_xref="MIM:616747"
     exon            1..495
                     /gene="CHPT1"
                     /gene_synonym="CPT; CPT1"
                     /inference="alignment:Splign:2.1.0"
     CDS             223..1443
                     /gene="CHPT1"
                     /gene_synonym="CPT; CPT1"
                     /EC_number="2.7.8.2"
                     /note="cholinephosphotransferase 1 alpha;
                     phosphatidylcholine synthesizing enzyme; hCPT1; AAPT1-like
                     protein; diacylglycerol cholinephosphotransferase 1"
                     /codon_start=1
                     /product="cholinephosphotransferase 1"
                     /protein_id="NP_064629.2"
                     /db_xref="CCDS:CCDS9086.1"
                     /db_xref="GeneID:56994"
                     /db_xref="HGNC:HGNC:17852"
                     /db_xref="MIM:616747"
                     /translation="MAAGAGAGSAPRWLRALSEPLSAAQLRRLEEHRYSAAGVSLLEP
                     PLQLYWTWLLQWIPLWMAPNSITLLGLAVNVVTTLVLISYCPTATEEAPYWTYLLCAL
                     GLFIYQSLDAIDGKQARRTNSCSPLGELFDHGCDSLSTVFMAVGASIAARLGTYPDWF
                     FFCSFIGMFVFYCAHWQTYVSGMLRFGKVDVTEIQIALVIVFVLSAFGGATMWDYTIP
                     ILEIKLKILPVLGFLGGVIFSCSNYFHVILHGGVGKNGSTIAGTSVLSPGLHIGLIII
                     LAIMIYKKSATDVFEKHPCLYILMFGCVFAKVSQKLVVAHMTKSELYLQDTVFLGPGL
                     LFLDQYFNNFIDEYVVLWMAMVISSFDMVIYFSALCLQISRHLHLNIFKTACHQAPEQ
                     VQVLSSKSHQNNMD"
     exon            496..643
                     /gene="CHPT1"
                     /gene_synonym="CPT; CPT1"
                     /inference="alignment:Splign:2.1.0"
     exon            644..785
                     /gene="CHPT1"
                     /gene_synonym="CPT; CPT1"
                     /inference="alignment:Splign:2.1.0"
     exon            786..870
                     /gene="CHPT1"
                     /gene_synonym="CPT; CPT1"
                     /inference="alignment:Splign:2.1.0"
     exon            871..1002
                     /gene="CHPT1"
                     /gene_synonym="CPT; CPT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1003..1161
                     /gene="CHPT1"
                     /gene_synonym="CPT; CPT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1162..1287
                     /gene="CHPT1"
                     /gene_synonym="CPT; CPT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1288..1398
                     /gene="CHPT1"
                     /gene_synonym="CPT; CPT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1399..1572
                     /gene="CHPT1"
                     /gene_synonym="CPT; CPT1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1546..1551
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CHPT1"
                     /gene_synonym="CPT; CPT1"
                     /note="hexamer: AATAAA"
     polyA_site      1572
                     /gene="CHPT1"
                     /gene_synonym="CPT; CPT1"
                     /note="major polyA site"
ORIGIN      
        1 gccccacccg cgagccgcag ccgcggcccc acagcttctg gggctggggc cccggcagcc
       61 gggcaggccg gcctgacctc gacctccgcc gtgcgggccc gaccggtgag tccagcccgg
      121 cagtcgcagg acccggccgc cagcctctcc ctccacctct ccctgccccc agcgccaggc
      181 gcgggctgcg ctcggtggcg gcggcggggc cctcaggcgg ccatggcggc aggcgccggg
      241 gccgggtccg cgccgcgctg gctgagggcg ctgagcgagc cgctgagcgc ggcgcagctg
      301 cggcgactgg aggagcaccg ctacagcgcg gcgggcgtct cgctgctcga gccgccgctg
      361 cagctctact ggacctggct gctccagtgg atcccgctct ggatggcccc caactccatc
      421 accctgctgg ggctcgccgt caacgtggtc accacgctcg tgctcatctc ctactgtccc
      481 acggccaccg aagaggcacc atactggaca taccttttat gtgcactggg actttttatt
      541 taccagtcac tggatgctat tgatgggaaa caagccagaa gaacaaactc ttgttcccct
      601 ttaggggagc tctttgacca tggctgtgac tctctttcca cagtatttat ggcagtggga
      661 gcttcaattg ccgctcgctt aggaacttat cctgactggt tttttttctg ctcttttatt
      721 gggatgtttg tgttttattg cgctcattgg cagacttatg tttcaggcat gttgagattt
      781 ggaaaagtgg atgtaactga aattcagata gctttagtga ttgtctttgt gttgtctgca
      841 tttggaggag caacaatgtg ggactatacg attcctattc tagaaataaa attgaagatc
      901 cttccagttc ttggatttct aggtggagta atattttcct gttcaaatta tttccatgtt
      961 atcctccatg gtggtgttgg caagaatgga tccactatag caggcaccag tgtcttgtca
     1021 cctggactcc acataggact aattattata ctggcaataa tgatctataa aaagtcagca
     1081 actgatgtgt ttgaaaagca tccttgtctt tatatcctaa tgtttggatg tgtctttgct
     1141 aaagtctcac aaaaattagt ggtagctcac atgaccaaaa gtgaactata tcttcaagac
     1201 actgtctttt tggggccagg tcttttgttt ttagaccagt actttaataa ctttatagac
     1261 gaatatgttg ttctatggat ggcaatggtg atttcttcat ttgatatggt gatatacttt
     1321 agtgctttgt gcctgcaaat ttcaagacac cttcatctaa atatattcaa gactgcatgt
     1381 catcaagcac ctgaacaggt tcaagttctt tcttcaaaga gtcatcagaa taacatggat
     1441 tgaagagact tccgaacact tgctatctct tgctgctgct gtttcatgga aggagatatt
     1501 aaacatttgt ttaattttta tttaagtgtt atacctattt cagcaaataa aatatttcat
     1561 tgcttatatt aa
//
