LOCUS       NM_019015               3989 bp    mRNA    linear   PRI 23-MAR-2024
DEFINITION  Homo sapiens chondroitin polymerizing factor 2 (CHPF2), transcript
            variant 1, mRNA.
ACCESSION   NM_019015 XM_376724 XM_379974
VERSION     NM_019015.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3989)
  AUTHORS   Han,L., Zhou,H., Guo,Z., Jiang,C., Wang,Z., Zhang,H. and Liu,D.
  TITLE     Exosomal lncRNA DUXAP8 affecting CHPF2 in the pathogenesis of
            intracranial aneurysms
  JOURNAL   Gene 908, 148253 (2024)
   PUBMED   38341004
  REMARK    GeneRIF: Exosomal lncRNA DUXAP8 affecting CHPF2 in the pathogenesis
            of intracranial aneurysms.
REFERENCE   2  (bases 1 to 3989)
  AUTHORS   Wen,X., Qin,J., Zhang,X., Ye,L., Wang,Y., Yang,R., Di,Y., He,W. and
            Wang,Z.
  TITLE     MEK-mediated CHPF2 phosphorylation promotes colorectal cancer cell
            proliferation and metastasis by activating NF-kappaB signaling
  JOURNAL   Cancer Lett 584, 216644 (2024)
   PUBMED   38253217
  REMARK    GeneRIF: MEK-mediated CHPF2 phosphorylation promotes colorectal
            cancer cell proliferation and metastasis by activating NF-kappaB
            signaling.
REFERENCE   3  (bases 1 to 3989)
  AUTHORS   Kalathas,D., Theocharis,D.A., Bounias,D., Kyriakopoulou,D.,
            Papageorgakopoulou,N., Stavropoulos,M.S. and Vynios,D.H.
  TITLE     Chondroitin synthases I, II, III and chondroitin sulfate
            glucuronyltransferase expression in colorectal cancer
  JOURNAL   Mol Med Rep 4 (2), 363-368 (2011)
   PUBMED   21468578
  REMARK    GeneRIF: The present study focused on the expression of
            chondroitin-synthesizing enzymes in colorectal cancer.
REFERENCE   4  (bases 1 to 3989)
  AUTHORS   Ghosh,D., Lippert,D., Krokhin,O., Cortens,J.P. and Wilkins,J.A.
  TITLE     Defining the membrane proteome of NK cells
  JOURNAL   J Mass Spectrom 45 (1), 1-25 (2010)
   PUBMED   19946888
REFERENCE   5  (bases 1 to 3989)
  AUTHORS   Izumikawa,T., Koike,T., Shiozawa,S., Sugahara,K., Tamura,J. and
            Kitagawa,H.
  TITLE     Identification of chondroitin sulfate glucuronyltransferase as
            chondroitin synthase-3 involved in chondroitin polymerization:
            chondroitin polymerization is achieved by multiple enzyme complexes
            consisting of chondroitin synthase family members
  JOURNAL   J Biol Chem 283 (17), 11396-11406 (2008)
   PUBMED   18316376
  REMARK    GeneRIF: chondroitin polymerization is achieved by multiple
            combinations of ChSy-1, ChSy-2, CSGlcA-T, and ChPF and each
            combination may play a unique role in the biosynthesis of CS;
            CSGlcA-T is identified as chondroitin synthase-3 (ChSy-3)
REFERENCE   6  (bases 1 to 3989)
  AUTHORS   Clark,H.F., Gurney,A.L., Abaya,E., Baker,K., Baldwin,D., Brush,J.,
            Chen,J., Chow,B., Chui,C., Crowley,C., Currell,B., Deuel,B.,
            Dowd,P., Eaton,D., Foster,J., Grimaldi,C., Gu,Q., Hass,P.E.,
            Heldens,S., Huang,A., Kim,H.S., Klimowski,L., Jin,Y., Johnson,S.,
            Lee,J., Lewis,L., Liao,D., Mark,M., Robbie,E., Sanchez,C.,
            Schoenfeld,J., Seshagiri,S., Simmons,L., Singh,J., Smith,V.,
            Stinson,J., Vagts,A., Vandlen,R., Watanabe,C., Wieand,D., Woods,K.,
            Xie,M.H., Yansura,D., Yi,S., Yu,G., Yuan,J., Zhang,M., Zhang,Z.,
            Goddard,A., Wood,W.I., Godowski,P. and Gray,A.
  TITLE     The secreted protein discovery initiative (SPDI), a large-scale
            effort to identify novel human secreted and transmembrane proteins:
            a bioinformatics assessment
  JOURNAL   Genome Res 13 (10), 2265-2270 (2003)
   PUBMED   12975309
  REMARK    Erratum:[Genome Res. 2003 Dec;13(12):2759]
REFERENCE   7  (bases 1 to 3989)
  AUTHORS   Gotoh,M., Yada,T., Sato,T., Akashima,T., Iwasaki,H., Mochizuki,H.,
            Inaba,N., Togayachi,A., Kudo,T., Watanabe,H., Kimata,K. and
            Narimatsu,H.
  TITLE     Molecular cloning and characterization of a novel chondroitin
            sulfate glucuronyltransferase that transfers glucuronic acid to
            N-acetylgalactosamine
  JOURNAL   J Biol Chem 277 (41), 38179-38188 (2002)
   PUBMED   12145278
  REMARK    GeneRIF: Results describe a novel human gene that possesses
            homology with chondroitin synthase.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC021097.5.
            
            On Sep 18, 2020 this sequence version replaced NM_019015.2.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC052944.1, AB095812.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000035307.7/ ENSP00000035307.2
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1792              AC021097.5         7121-8912           c
            1793-2357           AC021097.5         5783-6347           c
            2358-2540           AC021097.5         4805-4987           c
            2541-3989           AC021097.5         2573-4021           c
FEATURES             Location/Qualifiers
     source          1..3989
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7q36.1"
     gene            1..3989
                     /gene="CHPF2"
                     /gene_synonym="chPF-2; ChSy-3; CSGLCA-T; CSGlcAT"
                     /note="chondroitin polymerizing factor 2"
                     /db_xref="GeneID:54480"
                     /db_xref="HGNC:HGNC:29270"
                     /db_xref="MIM:608037"
     exon            1..1792
                     /gene="CHPF2"
                     /gene_synonym="chPF-2; ChSy-3; CSGLCA-T; CSGlcAT"
                     /inference="alignment:Splign:2.1.0"
     CDS             1530..3848
                     /gene="CHPF2"
                     /gene_synonym="chPF-2; ChSy-3; CSGLCA-T; CSGlcAT"
                     /EC_number="2.4.1.226"
                     /note="isoform 1 is encoded by transcript variant 1;
                     chondroitin sulfate glucuronyltransferase; chondroitin
                     synthase-3; N-acetylgalactosaminyl-proteoglycan
                     3-beta-glucuronosyltransferase"
                     /codon_start=1
                     /product="chondroitin sulfate glucuronyltransferase
                     isoform 1"
                     /protein_id="NP_061888.1"
                     /db_xref="CCDS:CCDS34779.1"
                     /db_xref="GeneID:54480"
                     /db_xref="HGNC:HGNC:29270"
                     /db_xref="MIM:608037"
                     /translation="MRLSSLLALLRPALPLILGLSLGCSLSLLRVSWIQGEGEDPCVE
                     AVGERGGPQNPDSRARLDQSDEDFKPRIVPYYRDPNKPYKKVLRTRYIQTELGSRERL
                     LVAVLTSRATLSTLAVAVNRTVAHHFPRLLYFTGQRGARAPAGMQVVSHGDERPAWLM
                     SETLRHLHTHFGADYDWFFIMQDDTYVQAPRLAALAGHLSINQDLYLGRAEEFIGAGE
                     QARYCHGGFGYLLSRSLLLRLRPHLDGCRGDILSARPDEWLGRCLIDSLGVGCVSQHQ
                     GQQYRSFELAKNRDPEKEGSSAFLSAFAVHPVSEGTLMYRLHKRFSALELERAYSEIE
                     QLQAQIRNLTVLTPEGEAGLSWPVGLPAPFTPHSRFEVLGWDYFTEQHTFSCADGAPK
                     CPLQGASRADVGDALETALEQLNRRYQPRLRFQKQRLLNGYRRFDPARGMEYTLDLLL
                     ECVTQRGHRRALARRVSLLRPLSRVEILPMPYVTEATRVQLVLPLLVAEAAAAPAFLE
                     AFAANVLEPREHALLTLLLVYGPREGGRGAPDPFLGVKAAAAELERRYPGTRLAWLAV
                     RAEAPSQVRLMDVVSKKHPVDTLFFLTTVWTRPGPEVLNRCRMNAISGWQAFFPVHFQ
                     EFNPALSPQRSPPGPPGAGPDPPSPPGADPSRGAPIGGRFDRQASAEGCFYNADYLAA
                     RARLAGELAGQEEEEALEGLEVMDVFLRFSGLHLFRAVEPGLVQKFSLRDCSPRLSEE
                     LYHRCRLSNLEGLGGRAQLAMALFEQEQANST"
     exon            1793..2357
                     /gene="CHPF2"
                     /gene_synonym="chPF-2; ChSy-3; CSGLCA-T; CSGlcAT"
                     /inference="alignment:Splign:2.1.0"
     exon            2358..2540
                     /gene="CHPF2"
                     /gene_synonym="chPF-2; ChSy-3; CSGLCA-T; CSGlcAT"
                     /inference="alignment:Splign:2.1.0"
     exon            2541..3989
                     /gene="CHPF2"
                     /gene_synonym="chPF-2; ChSy-3; CSGLCA-T; CSGlcAT"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3968..3973
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CHPF2"
                     /gene_synonym="chPF-2; ChSy-3; CSGLCA-T; CSGlcAT"
                     /note="hexamer: ATTAAA"
     polyA_site      3989
                     /gene="CHPF2"
                     /gene_synonym="chPF-2; ChSy-3; CSGLCA-T; CSGlcAT"
                     /note="major polyA site"
ORIGIN      
        1 agaggaagct gcggacaggg gctgtgaggt ggcagcggct gcagcggcgg agccggcgcc
       61 tcagcgggca ctggggtctg ttcccccttc cccgtccctg ctccctgcca ggcgcgtgcg
      121 ggacgccgct cttggtcccc acgcctccgc cccgccccct cccgggacgc cgggagaccc
      181 cggccgtcct ttatccggtg tccgccggcc cccggccctg aaacccgggc ctcctccccg
      241 agggccttgg gcgtccctcc tggtcctgcg ctcgcggcct cgatgctgtc tctggcgcgg
      301 cctccgctcc cgccgactgg cctgagaacg aggtctgtgc cccagtctcc cagccgcgac
      361 ctccgacccc gcctcgcaga acgacccgag ctggtctccc gagccccctt ctcagcagcc
      421 cggtgacgtg gccagtttat ttctgttttg agacgaacgg cgaagactcg cgtcgggtct
      481 tcgttctagg gctagacttg gtctctgatc gccgagaggt agcgcagggg ctgtgggccc
      541 ccggcagggg tcctgtcgga agctggccgc gcttctgttt gcgttcccag gaccctggca
      601 ttgtctctag ttgctgcttg tgctctctct ttgcttttgg tttgcttcat ttggcccctg
      661 gggccctggt aaaaccaggc accgaatcgc tcgcacacag agttccagtc cccgcctgct
      721 gtctcctcag cagctggggt tccgaggaga atgccctgca agatggctcc atcggccatg
      781 gcgctcctga gaggctgtca gtgctgagtc accgatctac ctcattcggg tgggcagaac
      841 ttatgtgtgc catgcgagtg gctccagacc gctcctgagt gggaggaggg gttcctgtag
      901 ccgttgcgtc ttctcaaaca cggggagcag agtagaaaga ggctctggcc ccttcccttg
      961 tgcccaccgg gcctgccgca gtggctcagc agccccttca gtagcccgcc tgaggaccga
     1021 tgccagaggc aggcattctt ccggaaaggc ccactgaggc aggctccggc tcctctggtt
     1081 ggggctgttg ttttgatgga tcgtgtgctt ttcccttacc tcttatcact tgctgtcatc
     1141 tgttgactta ggcccagtct gcagatgtgt gtagtgttcc tttttgggtt agctttggca
     1201 gtattgagtt ttacttcctc ctctttttag tggaagacag accataatcc cagtgtgagt
     1261 gaaattgatt gtttcattta ttaccgtttt ggctgggggt tagttccgac accttcacag
     1321 ttgaagagca ggcagaagga gttgtgaaga caggacaatc ttcttgggga tgctggtcct
     1381 ggaagccagc gggcctcgct ctgtctttgg cctcattgac cccaggttct ctggttaaaa
     1441 ctgaaagcct actactggcc tggtgcccat caatccattg atccttgagg ctgtgcccct
     1501 ggggcaccca cctggcaggg cctaccacca tgcgactgag ctccctgttg gctctgctgc
     1561 ggccagcgct tcccctcatc ttagggctgt ctctggggtg cagcctgagc ctcctgcggg
     1621 tttcctggat ccagggggag ggagaagatc cctgtgtcga ggctgtaggg gagcgaggag
     1681 ggccacagaa tccagattcc agagctcggc tagaccaaag tgatgaagac ttcaaacccc
     1741 ggattgtccc ctactacagg gaccccaaca agccctacaa gaaggtgctc aggactcggt
     1801 acatccagac agagctgggc tcccgtgagc ggttgctggt ggctgtcctg acctcccgag
     1861 ctacactgtc cactttggcc gtggctgtga accgtacggt ggcccatcac ttccctcggt
     1921 tactctactt cactgggcag cggggggccc gggctccagc agggatgcag gtggtgtctc
     1981 atggggatga gcggcccgcc tggctcatgt cagagaccct gcgccacctt cacacacact
     2041 ttggggccga ctacgactgg ttcttcatca tgcaggatga cacatatgtg caggcccccc
     2101 gcctggcagc ccttgctggc cacctcagca tcaaccaaga cctgtactta ggccgggcag
     2161 aggagttcat tggcgcaggc gagcaggccc ggtactgtca tgggggcttt ggctacctgt
     2221 tgtcacggag tctcctgctt cgtctgcggc cacatctgga tggctgccga ggagacattc
     2281 tcagtgcccg tcctgacgag tggcttggac gctgcctcat tgactctctg ggcgtcggct
     2341 gtgtctcaca gcaccagggg cagcagtatc gctcatttga actggccaaa aatagggacc
     2401 ctgagaagga agggagctcg gctttcctga gtgccttcgc cgtgcaccct gtctccgaag
     2461 gtaccctcat gtaccggctc cacaaacgct tcagcgctct ggagttggag cgggcttaca
     2521 gtgaaataga acaactgcag gctcagatcc ggaacctgac cgtgctgacc cccgaagggg
     2581 aggcagggct gagctggccc gttgggctcc ctgctccttt cacaccacac tctcgctttg
     2641 aggtgctggg ctgggactac ttcacagagc agcacacctt ctcctgtgca gatggggctc
     2701 ccaagtgccc actacagggg gctagcaggg cggacgtggg tgatgcgttg gagactgccc
     2761 tggagcagct caatcggcgc tatcagcccc gcctgcgctt ccagaagcag cgactgctca
     2821 acggctatcg gcgcttcgac ccagcacggg gcatggagta caccctggac ctgctgttgg
     2881 aatgtgtgac acagcgtggg caccggcggg ccctggctcg cagggtcagc ctgctgcggc
     2941 cactgagccg ggtggaaatc ctacctatgc cctatgtcac tgaggccacc cgagtgcagc
     3001 tggtgctgcc actcctggtg gctgaagctg ctgcagcccc ggctttcctc gaggcctttg
     3061 cagccaatgt cctggagcca cgagaacatg cattgctcac cctgttgctg gtctacgggc
     3121 cacgagaagg tggccgtgga gctccagacc catttcttgg ggtgaaggct gcagcagcgg
     3181 agttagagcg acggtaccct gggacgaggc tggcctggct cgctgtgcga gcagaggccc
     3241 cttcccaggt gcgactcatg gacgtggtct cgaagaagca ccctgtggac actctcttct
     3301 tccttaccac cgtgtggaca aggcctgggc ccgaagtcct caaccgctgt cgcatgaatg
     3361 ccatctctgg ctggcaggcc ttctttccag tccatttcca ggagttcaat cctgccctgt
     3421 caccacagag atcaccccca gggcccccgg gggctggccc tgaccccccc tcccctcctg
     3481 gtgctgaccc ctcccggggg gctcctatag gggggagatt tgaccggcag gcttctgcgg
     3541 agggctgctt ctacaacgct gactacctgg cggcccgagc ccggctggca ggtgaactgg
     3601 caggccagga agaggaggaa gccctggagg ggctggaggt gatggatgtt ttcctccggt
     3661 tctcagggct ccacctcttt cgggccgtag agccagggct ggtgcagaag ttctccctgc
     3721 gagactgcag cccacggctc agtgaagaac tctaccaccg ctgccgcctc agcaacctgg
     3781 aggggctagg gggccgtgcc cagctggcta tggctctctt tgagcaggag caggccaata
     3841 gcacttagcc cgcctggggg ccctaacctc attacctttc ctttgtctgc ctcagcccca
     3901 ggaagggcaa ggcaagatgg tggacagata gagaattgtt gctgtatttt ttaaatatga
     3961 aaatgttatt aaacatgtct tctgccaaa
//
