LOCUS       NP_061888                772 aa            linear   PRI 30-APR-2025
DEFINITION  chondroitin sulfate glucuronyltransferase isoform 1 [Homo sapiens].
ACCESSION   NP_061888 XP_376724 XP_379974
VERSION     NP_061888.1
DBSOURCE    REFSEQ: accession NM_019015.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (residues 1 to 772)
  AUTHORS   Han,L., Zhou,H., Guo,Z., Jiang,C., Wang,Z., Zhang,H. and Liu,D.
  TITLE     Exosomal lncRNA DUXAP8 affecting CHPF2 in the pathogenesis of
            intracranial aneurysms
  JOURNAL   Gene 908, 148253 (2024)
   PUBMED   38341004
  REMARK    GeneRIF: Exosomal lncRNA DUXAP8 affecting CHPF2 in the pathogenesis
            of intracranial aneurysms.
REFERENCE   2  (residues 1 to 772)
  AUTHORS   Wen,X., Qin,J., Zhang,X., Ye,L., Wang,Y., Yang,R., Di,Y., He,W. and
            Wang,Z.
  TITLE     MEK-mediated CHPF2 phosphorylation promotes colorectal cancer cell
            proliferation and metastasis by activating NF-kappaB signaling
  JOURNAL   Cancer Lett 584, 216644 (2024)
   PUBMED   38253217
  REMARK    GeneRIF: MEK-mediated CHPF2 phosphorylation promotes colorectal
            cancer cell proliferation and metastasis by activating NF-kappaB
            signaling.
REFERENCE   3  (residues 1 to 772)
  AUTHORS   Kalathas,D., Theocharis,D.A., Bounias,D., Kyriakopoulou,D.,
            Papageorgakopoulou,N., Stavropoulos,M.S. and Vynios,D.H.
  TITLE     Chondroitin synthases I, II, III and chondroitin sulfate
            glucuronyltransferase expression in colorectal cancer
  JOURNAL   Mol Med Rep 4 (2), 363-368 (2011)
   PUBMED   21468578
  REMARK    GeneRIF: The present study focused on the expression of
            chondroitin-synthesizing enzymes in colorectal cancer.
REFERENCE   4  (residues 1 to 772)
  AUTHORS   Ghosh,D., Lippert,D., Krokhin,O., Cortens,J.P. and Wilkins,J.A.
  TITLE     Defining the membrane proteome of NK cells
  JOURNAL   J Mass Spectrom 45 (1), 1-25 (2010)
   PUBMED   19946888
REFERENCE   5  (residues 1 to 772)
  AUTHORS   Izumikawa,T., Koike,T., Shiozawa,S., Sugahara,K., Tamura,J. and
            Kitagawa,H.
  TITLE     Identification of chondroitin sulfate glucuronyltransferase as
            chondroitin synthase-3 involved in chondroitin polymerization:
            chondroitin polymerization is achieved by multiple enzyme complexes
            consisting of chondroitin synthase family members
  JOURNAL   J Biol Chem 283 (17), 11396-11406 (2008)
   PUBMED   18316376
  REMARK    GeneRIF: chondroitin polymerization is achieved by multiple
            combinations of ChSy-1, ChSy-2, CSGlcA-T, and ChPF and each
            combination may play a unique role in the biosynthesis of CS;
            CSGlcA-T is identified as chondroitin synthase-3 (ChSy-3)
REFERENCE   6  (residues 1 to 772)
  AUTHORS   Clark,H.F., Gurney,A.L., Abaya,E., Baker,K., Baldwin,D., Brush,J.,
            Chen,J., Chow,B., Chui,C., Crowley,C., Currell,B., Deuel,B.,
            Dowd,P., Eaton,D., Foster,J., Grimaldi,C., Gu,Q., Hass,P.E.,
            Heldens,S., Huang,A., Kim,H.S., Klimowski,L., Jin,Y., Johnson,S.,
            Lee,J., Lewis,L., Liao,D., Mark,M., Robbie,E., Sanchez,C.,
            Schoenfeld,J., Seshagiri,S., Simmons,L., Singh,J., Smith,V.,
            Stinson,J., Vagts,A., Vandlen,R., Watanabe,C., Wieand,D., Woods,K.,
            Xie,M.H., Yansura,D., Yi,S., Yu,G., Yuan,J., Zhang,M., Zhang,Z.,
            Goddard,A., Wood,W.I., Godowski,P. and Gray,A.
  TITLE     The secreted protein discovery initiative (SPDI), a large-scale
            effort to identify novel human secreted and transmembrane proteins:
            a bioinformatics assessment
  JOURNAL   Genome Res 13 (10), 2265-2270 (2003)
   PUBMED   12975309
  REMARK    Erratum:[Genome Res. 2003 Dec;13(12):2759]
REFERENCE   7  (residues 1 to 772)
  AUTHORS   Gotoh,M., Yada,T., Sato,T., Akashima,T., Iwasaki,H., Mochizuki,H.,
            Inaba,N., Togayachi,A., Kudo,T., Watanabe,H., Kimata,K. and
            Narimatsu,H.
  TITLE     Molecular cloning and characterization of a novel chondroitin
            sulfate glucuronyltransferase that transfers glucuronic acid to
            N-acetylgalactosamine
  JOURNAL   J Biol Chem 277 (41), 38179-38188 (2002)
   PUBMED   12145278
  REMARK    GeneRIF: Results describe a novel human gene that possesses
            homology with chondroitin synthase.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC021097.5.
            
            On or before Aug 30, 2004 this sequence version replaced
            XP_376724.1, XP_379974.1.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC052944.1, AB095812.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000035307.7/ ENSP00000035307.2
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..772
                     /organism="Homo sapiens"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7q36.1"
     Protein         1..772
                     /product="chondroitin sulfate glucuronyltransferase
                     isoform 1"
                     /EC_number="2.4.1.226"
                     /note="chondroitin sulfate glucuronyltransferase;
                     chondroitin synthase-3;
                     N-acetylgalactosaminyl-proteoglycan
                     3-beta-glucuronosyltransferase"
                     /calculated_mol_wt=85817
     Site            7..29
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9P2E5.2)"
     Site            121
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q9P2E5.2)"
     Region          <177..>269
                     /region_name="Galactosyl_T"
                     /note="Galactosyltransferase; cl21608"
                     /db_xref="CDD:473923"
     Region          242..759
                     /region_name="CHGN"
                     /note="Chondroitin N-acetylgalactosaminyltransferase;
                     pfam05679"
                     /db_xref="CDD:461712"
     Site            342
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q9P2E5.2)"
     Region          629..662
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9P2E5.2)"
     CDS             1..772
                     /gene="CHPF2"
                     /gene_synonym="chPF-2; ChSy-3; CSGLCA-T; CSGlcAT"
                     /coded_by="NM_019015.3:1530..3848"
                     /note="isoform 1 is encoded by transcript variant 1"
                     /db_xref="CCDS:CCDS34779.1"
                     /db_xref="GeneID:54480"
                     /db_xref="HGNC:HGNC:29270"
                     /db_xref="MIM:608037"
ORIGIN      
        1 mrlssllall rpalplilgl slgcslsllr vswiqgeged pcveavgerg gpqnpdsrar
       61 ldqsdedfkp rivpyyrdpn kpykkvlrtr yiqtelgsre rllvavltsr atlstlavav
      121 nrtvahhfpr llyftgqrga rapagmqvvs hgderpawlm setlrhlhth fgadydwffi
      181 mqddtyvqap rlaalaghls inqdlylgra eefigageqa rychggfgyl lsrslllrlr
      241 phldgcrgdi lsarpdewlg rclidslgvg cvsqhqgqqy rsfelaknrd pekegssafl
      301 safavhpvse gtlmyrlhkr fsaleleray seieqlqaqi rnltvltpeg eaglswpvgl
      361 papftphsrf evlgwdyfte qhtfscadga pkcplqgasr advgdaleta leqlnrryqp
      421 rlrfqkqrll ngyrrfdpar gmeytldlll ecvtqrghrr alarrvsllr plsrveilpm
      481 pyvteatrvq lvlpllvaea aaapafleaf aanvlepreh alltlllvyg preggrgapd
      541 pflgvkaaaa elerrypgtr lawlavraea psqvrlmdvv skkhpvdtlf flttvwtrpg
      601 pevlnrcrmn aisgwqaffp vhfqefnpal spqrsppgpp gagpdppspp gadpsrgapi
      661 ggrfdrqasa egcfynadyl aararlagel agqeeeeale glevmdvflr fsglhlfrav
      721 epglvqkfsl rdcsprlsee lyhrcrlsnl eglggraqla malfeqeqan st
//
