LOCUS       NM_024536               3028 bp    mRNA    linear   PRI 11-JUN-2024
DEFINITION  Homo sapiens chondroitin polymerizing factor (CHPF), transcript
            variant 1, mRNA.
ACCESSION   NM_024536
VERSION     NM_024536.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3028)
  AUTHORS   Huang,Y., Zhang,Z., Tong,H., Qin,W., Li,Q., Ma,L., Ren,Z., Chen,W.,
            Zhang,Y., Zhong,Y., Yao,L. and Zhou,P.
  TITLE     Chondroitin polymerizing factor promotes development and
            progression of colorectal cancer via facilitating transcription of
            VEGFB
  JOURNAL   J Cell Mol Med 28 (10), e18268 (2024)
   PUBMED   38775031
  REMARK    GeneRIF: Chondroitin polymerizing factor promotes development and
            progression of colorectal cancer via facilitating transcription of
            VEGFB.
REFERENCE   2  (bases 1 to 3028)
  AUTHORS   Yun,Z.Y., Wu,D., Wang,X., Huang,P. and Li,N.
  TITLE     MiR-214-3p overexpression-triggered chondroitin polymerizing factor
            (CHPF) inhibition modulates the ferroptosis and metabolism in colon
            cancer
  JOURNAL   Kaohsiung J Med Sci 40 (3), 244-254 (2024)
   PUBMED   38190270
  REMARK    GeneRIF: MiR-214-3p overexpression-triggered chondroitin
            polymerizing factor (CHPF) inhibition modulates the ferroptosis and
            metabolism in colon cancer.
REFERENCE   3  (bases 1 to 3028)
  AUTHORS   Wu,J., Wang,M., Zhang,Y., Liu,G. and Xing,Y.
  TITLE     Activation of CHPF by transcription factor NFIC promotes NLRP3
            activation during the progression of colorectal cancer
  JOURNAL   Funct Integr Genomics 24 (1), 20 (2024)
   PUBMED   38267731
  REMARK    GeneRIF: Activation of CHPF by transcription factor NFIC promotes
            NLRP3 activation during the progression of colorectal cancer.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3028)
  AUTHORS   Luo,H., Huang,K., Cheng,M., Long,X., Zhu,X. and Wu,M.
  TITLE     The HNF4A-CHPF pathway promotes proliferation and invasion through
            interactions with MAD1L1 in glioma
  JOURNAL   Aging (Albany NY) 15 (20), 11052-11066 (2023)
   PUBMED   37851364
  REMARK    GeneRIF: The HNF4A-CHPF pathway promotes proliferation and invasion
            through interactions with MAD1L1 in glioma.
REFERENCE   5  (bases 1 to 3028)
  AUTHORS   Sha,M., Zhang,S., Beejadhursing,R., Sun,Y., Qin,Y., Chen,S. and
            Li,W.
  TITLE     Extracellular vesicles derived from hypoxic HTR-8/SVneo trophoblast
            inhibit endothelial cell functions through the miR-150-3p /CHPF
            pathway
  JOURNAL   Placenta 138, 21-32 (2023)
   PUBMED   37156185
  REMARK    GeneRIF: Extracellular vesicles derived from hypoxic HTR-8/SVneo
            trophoblast inhibit endothelial cell functions through the
            miR-150-3p /CHPF pathway.
REFERENCE   6  (bases 1 to 3028)
  AUTHORS   Colland,F., Jacq,X., Trouplin,V., Mougin,C., Groizeleau,C.,
            Hamburger,A., Meil,A., Wojcik,J., Legrain,P. and Gauthier,J.M.
  TITLE     Functional proteomics mapping of a human signaling pathway
  JOURNAL   Genome Res 14 (7), 1324-1332 (2004)
   PUBMED   15231748
REFERENCE   7  (bases 1 to 3028)
  AUTHORS   Lehner,B. and Sanderson,C.M.
  TITLE     A protein interaction framework for human mRNA degradation
  JOURNAL   Genome Res 14 (7), 1315-1323 (2004)
   PUBMED   15231747
REFERENCE   8  (bases 1 to 3028)
  AUTHORS   Clark,H.F., Gurney,A.L., Abaya,E., Baker,K., Baldwin,D., Brush,J.,
            Chen,J., Chow,B., Chui,C., Crowley,C., Currell,B., Deuel,B.,
            Dowd,P., Eaton,D., Foster,J., Grimaldi,C., Gu,Q., Hass,P.E.,
            Heldens,S., Huang,A., Kim,H.S., Klimowski,L., Jin,Y., Johnson,S.,
            Lee,J., Lewis,L., Liao,D., Mark,M., Robbie,E., Sanchez,C.,
            Schoenfeld,J., Seshagiri,S., Simmons,L., Singh,J., Smith,V.,
            Stinson,J., Vagts,A., Vandlen,R., Watanabe,C., Wieand,D., Woods,K.,
            Xie,M.H., Yansura,D., Yi,S., Yu,G., Yuan,J., Zhang,M., Zhang,Z.,
            Goddard,A., Wood,W.I., Godowski,P. and Gray,A.
  TITLE     The secreted protein discovery initiative (SPDI), a large-scale
            effort to identify novel human secreted and transmembrane proteins:
            a bioinformatics assessment
  JOURNAL   Genome Res 13 (10), 2265-2270 (2003)
   PUBMED   12975309
  REMARK    Erratum:[Genome Res. 2003 Dec;13(12):2759]
REFERENCE   9  (bases 1 to 3028)
  AUTHORS   Yada,T., Gotoh,M., Sato,T., Shionyu,M., Go,M., Kaseyama,H.,
            Iwasaki,H., Kikuchi,N., Kwon,Y.D., Togayachi,A., Kudo,T.,
            Watanabe,H., Narimatsu,H. and Kimata,K.
  TITLE     Chondroitin sulfate synthase-2. Molecular cloning and
            characterization of a novel human glycosyltransferase homologous to
            chondroitin sulfate glucuronyltransferase, which has dual enzymatic
            activities
  JOURNAL   J Biol Chem 278 (32), 30235-30247 (2003)
   PUBMED   12761225
REFERENCE   10 (bases 1 to 3028)
  AUTHORS   Kitagawa,H., Izumikawa,T., Uyama,T. and Sugahara,K.
  TITLE     Molecular cloning of a chondroitin polymerizing factor that
            cooperates with chondroitin synthase for chondroitin polymerization
  JOURNAL   J Biol Chem 278 (26), 23666-23671 (2003)
   PUBMED   12716890
  REMARK    GeneRIF: chondroitin polymerizing activity requires concomitant
            expression of a ChPF with ChSy; coexpression of the ChPF and ChSy
            yielded markedly augmented glycosyltransferase activities, whereas
            simple mixing of the two separately expressed proteins did not.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC009955.4.
            
            On Nov 23, 2018 this sequence version replaced NM_024536.5.
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR3476690.369180.1,
                                           SRR3476690.908193.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA1970526
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000243776.11/ ENSP00000243776.6
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-585               AC009955.4         14114-14698         c
            586-1159            AC009955.4         12505-13078         c
            1160-1339           AC009955.4         11835-12014         c
            1340-3028           AC009955.4         9843-11531          c
FEATURES             Location/Qualifiers
     source          1..3028
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q35"
     gene            1..3028
                     /gene="CHPF"
                     /gene_synonym="CHPF1; CHSY2; CSS2"
                     /note="chondroitin polymerizing factor"
                     /db_xref="GeneID:79586"
                     /db_xref="HGNC:HGNC:24291"
                     /db_xref="MIM:610405"
     exon            1..585
                     /gene="CHPF"
                     /gene_synonym="CHPF1; CHSY2; CSS2"
                     /inference="alignment:Splign:2.1.0"
     CDS             272..2599
                     /gene="CHPF"
                     /gene_synonym="CHPF1; CHSY2; CSS2"
                     /EC_number="2.4.1.175"
                     /EC_number="2.4.1.226"
                     /note="isoform 1 is encoded by transcript variant 1;
                     chondroitin sulfate synthase 2;
                     N-acetylgalactosaminyltransferase 2; chondroitin
                     glucuronyltransferase 2;
                     N-acetylgalactosaminyl-proteoglycan
                     3-beta-glucuronosyltransferase II;
                     glucuronosyl-N-acetylgalactosaminyl-proteoglycan
                     4-beta-N-acetylgalactosaminyltransferase II"
                     /codon_start=1
                     /product="chondroitin sulfate synthase 2 isoform 1"
                     /protein_id="NP_078812.3"
                     /db_xref="CCDS:CCDS2443.1"
                     /db_xref="GeneID:79586"
                     /db_xref="HGNC:HGNC:24291"
                     /db_xref="MIM:610405"
                     /translation="MRASLLLSVLRPAGPVAVGISLGFTLSLLSVTWVEEPCGPGPPQ
                     PGDSELPPRGNTNAARRPNSVQPGAEREKPGAGEGAGENWEPRVLPYHPAQPGQAAKK
                     AVRTRYISTELGIRQRLLVAVLTSQTTLPTLGVAVNRTLGHRLERVVFLTGARGRRAP
                     PGMAVVTLGEERPIGHLHLALRHLLEQHGDDFDWFFLVPDTTYTEAHGLARLTGHLSL
                     ASAAHLYLGRPQDFIGGEPTPGRYCHGGFGVLLSRMLLQQLRPHLEGCRNDIVSARPD
                     EWLGRCILDATGVGCTGDHEGVHYSHLELSPGEPVQEGDPHFRSALTAHPVRDPVHMY
                     QLHKAFARAELERTYQEIQELQWEIQNTSHLAVDGDQAAAWPVGIPAPSRPASRFEVL
                     RWDYFTEQHAFSCADGSPRCPLRGADRADVADVLGTALEELNRRYHPALRLQKQQLVN
                     GYRRFDPARGMEYTLDLQLEALTPQGGRRPLTRRVQLLRPLSRVEILPVPYVTEASRL
                     TVLLPLAAAERDLAPGFLEAFATAALEPGDAAAALTLLLLYEPRQAQRVAHADVFAPV
                     KAHVAELERRFPGARVPWLSVQTAAPSPLRLMDLLSKKHPLDTLFLLAGPDTVLTPDF
                     LNRCRMHAISGWQAFFPMHFQAFHPAVAPPQGPGPPELGRDTGRFDRQAASEACFYNS
                     DYVAARGRLAAASEQEEELLESLDVYELFLHFSSLHVLRAVEPALLQRYRAQTCSARL
                     SEDLYHRCLQSVLEGLGSRTQLAMLLFEQEQGNST"
     exon            586..1159
                     /gene="CHPF"
                     /gene_synonym="CHPF1; CHSY2; CSS2"
                     /inference="alignment:Splign:2.1.0"
     exon            1160..1339
                     /gene="CHPF"
                     /gene_synonym="CHPF1; CHSY2; CSS2"
                     /inference="alignment:Splign:2.1.0"
     exon            1340..3028
                     /gene="CHPF"
                     /gene_synonym="CHPF1; CHSY2; CSS2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3008..3013
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CHPF"
                     /gene_synonym="CHPF1; CHSY2; CSS2"
                     /note="hexamer: AATAAA"
     polyA_site      3028
                     /gene="CHPF"
                     /gene_synonym="CHPF1; CHSY2; CSS2"
                     /note="major polyA site"
ORIGIN      
        1 gcagtgacac gtcggacagc tgcggccgcg gctgaggctc cagtgccgag cgcaagaacc
       61 ctgcgcagcc cagagcagct gctggagggg aatcgaggcg cggctccggg gattcggctc
      121 gggccgctgg ctctgctctg cggggaggga gcgggcccgc ccgcggggcc cgagccctcc
      181 ggatccgccc cctccccggt cccgccccct cggagactcc tctggctgct ctgggggttc
      241 gccggggccg gggacccgcg gtccgggcgc catgcgggca tcgctgctgc tgtcggtgct
      301 gcggcccgca gggcccgtgg ccgtgggcat ctccctgggc ttcaccctga gcctgctcag
      361 cgtcacctgg gtggaggagc cgtgcggccc aggcccgccc caacctggag actctgagct
      421 gccgccgcgc ggcaacacca acgcggcgcg ccggcccaac tcggtgcagc ccggagcgga
      481 gcgcgagaag cccggggccg gcgaaggcgc cggggagaat tgggagccgc gcgtcttgcc
      541 ctaccaccct gcacagcccg gccaggccgc caaaaaggcc gtcaggaccc gctacatcag
      601 cacggagctg ggcatcaggc agaggctgct ggtggcggtg ctgacctctc agaccacgct
      661 gcccacgctg ggcgtggccg tgaaccgcac gctggggcac cggctggagc gtgtggtgtt
      721 cctgacgggc gcacggggcc gccgggcccc acctggcatg gcagtggtga cgctaggcga
      781 ggagcgaccc attggacacc tgcacctggc gctgcgccac ctgctggagc agcacggcga
      841 cgactttgac tggttcttcc tggtgcctga caccacctac accgaggcgc acggcctggc
      901 acgcctaact ggccacctca gcctggcctc cgccgcccac ctgtacctgg gccggcccca
      961 ggacttcatc ggcggagagc ccacccccgg ccgctactgc cacggaggct ttggggtgct
     1021 gctgtcgcgc atgctgctgc aacaactgcg cccccacctg gaaggctgcc gcaacgacat
     1081 cgtcagtgcg cgccctgacg agtggctggg tcgctgcatt ctcgatgcca ccggggtggg
     1141 ctgcactggt gaccacgagg gggtgcacta tagccatctg gagctgagcc ctggggagcc
     1201 agtgcaggag ggggaccctc atttccgaag tgccctgaca gcccaccctg tgcgtgaccc
     1261 tgtgcacatg taccagctgc acaaagcttt cgcccgagct gaactggaac gcacgtacca
     1321 ggagatccag gagttacagt gggagatcca gaataccagc catctggccg ttgatgggga
     1381 ccaggcagct gcttggcccg tgggtattcc agcaccatcc cgcccggcct cccgctttga
     1441 ggtgctgcgc tgggactact tcacggagca gcacgctttc tcctgcgccg atggctcacc
     1501 ccgctgccca ctgcgtgggg ctgaccgggc tgatgtggcc gatgttctgg ggacagctct
     1561 agaggagctg aaccgccgct accacccggc cttgcggctc cagaagcagc agctggtgaa
     1621 tggctaccga cgctttgatc cggcccgggg tatggaatac acgctggact tgcagctgga
     1681 ggcactgacc ccccagggag gccgccggcc cctcactcgc cgagtgcagc tgctccggcc
     1741 gctgagccgc gtggagatct tgcctgtgcc ctatgtcact gaggcctcac gtctcactgt
     1801 gctgctgcct ctagctgcgg ctgagcgtga cctggcccct ggcttcttgg aggcctttgc
     1861 cactgcagca ctggagcctg gtgatgctgc ggcagccctg accctgctgc tactgtatga
     1921 gccgcgccag gcccagcgcg tggcccatgc agatgtcttc gcacctgtca aggcccacgt
     1981 ggcagagctg gagcggcgtt tccccggtgc ccgggtgcca tggctcagtg tgcagacagc
     2041 cgcaccctca ccactgcgcc tcatggatct actctccaag aagcacccgc tggacacact
     2101 gttcctgctg gccgggccag acacggtgct cacgcctgac ttcctgaacc gctgccgcat
     2161 gcatgccatc tccggctggc aggccttctt tcccatgcat ttccaagcct tccacccagc
     2221 tgtggcccca ccacaagggc ctgggccccc agagctgggc cgtgacactg gccgctttga
     2281 tcgccaggca gccagcgagg cctgcttcta caactccgac tatgtggcag cccgtgggcg
     2341 cctggcggca gcctcagaac aagaagagga gctgctggag agcctggatg tgtacgagct
     2401 gttcctccac ttctccagtc tgcatgtgct gcgggcggtg gagccggcgc tgctgcagcg
     2461 ctaccgggcc cagacgtgca gcgcgaggct cagtgaggac ctgtaccacc gctgcctcca
     2521 gagcgtgctt gagggcctcg gctcccgaac ccagctggcc atgctactct ttgaacagga
     2581 gcagggcaac agcacctgac cccaccctgt ccccgtgggc cgtggcatgg ccacacccca
     2641 ccccacttct cccccaaaac cagagccacc tgccagcctc gctgggcagg gctggccgta
     2701 gccagacccc aagctggccc actggtcccc tctctggctc tgtgggtccc tgggctctgg
     2761 acaagcactg ggggacgtgc ccccagagcc acccacttct catcccaaac ccagtttccc
     2821 tgccccctga cgctgctgat tcgggctgtg gcctccacgt atttatgcag tacagtctgc
     2881 ctgacgccag ccctgcctct gggccctggg ggctgggctg tagaagagtt gttggggaag
     2941 gagggagctg aggagggggc atctcccaac ttctcccttt tggaccctgc cgaagctccc
     3001 tgcctttaat aaactggcca agtgtgga
//
