LOCUS       NM_001195731            2628 bp    mRNA    linear   PRI 10-JUN-2024
DEFINITION  Homo sapiens chondroitin polymerizing factor (CHPF), transcript
            variant 2, mRNA.
ACCESSION   NM_001195731
VERSION     NM_001195731.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2628)
  AUTHORS   Huang,Y., Zhang,Z., Tong,H., Qin,W., Li,Q., Ma,L., Ren,Z., Chen,W.,
            Zhang,Y., Zhong,Y., Yao,L. and Zhou,P.
  TITLE     Chondroitin polymerizing factor promotes development and
            progression of colorectal cancer via facilitating transcription of
            VEGFB
  JOURNAL   J Cell Mol Med 28 (10), e18268 (2024)
   PUBMED   38775031
  REMARK    GeneRIF: Chondroitin polymerizing factor promotes development and
            progression of colorectal cancer via facilitating transcription of
            VEGFB.
REFERENCE   2  (bases 1 to 2628)
  AUTHORS   Yun,Z.Y., Wu,D., Wang,X., Huang,P. and Li,N.
  TITLE     MiR-214-3p overexpression-triggered chondroitin polymerizing factor
            (CHPF) inhibition modulates the ferroptosis and metabolism in colon
            cancer
  JOURNAL   Kaohsiung J Med Sci 40 (3), 244-254 (2024)
   PUBMED   38190270
  REMARK    GeneRIF: MiR-214-3p overexpression-triggered chondroitin
            polymerizing factor (CHPF) inhibition modulates the ferroptosis and
            metabolism in colon cancer.
REFERENCE   3  (bases 1 to 2628)
  AUTHORS   Wu,J., Wang,M., Zhang,Y., Liu,G. and Xing,Y.
  TITLE     Activation of CHPF by transcription factor NFIC promotes NLRP3
            activation during the progression of colorectal cancer
  JOURNAL   Funct Integr Genomics 24 (1), 20 (2024)
   PUBMED   38267731
  REMARK    GeneRIF: Activation of CHPF by transcription factor NFIC promotes
            NLRP3 activation during the progression of colorectal cancer.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2628)
  AUTHORS   Luo,H., Huang,K., Cheng,M., Long,X., Zhu,X. and Wu,M.
  TITLE     The HNF4A-CHPF pathway promotes proliferation and invasion through
            interactions with MAD1L1 in glioma
  JOURNAL   Aging (Albany NY) 15 (20), 11052-11066 (2023)
   PUBMED   37851364
  REMARK    GeneRIF: The HNF4A-CHPF pathway promotes proliferation and invasion
            through interactions with MAD1L1 in glioma.
REFERENCE   5  (bases 1 to 2628)
  AUTHORS   Sha,M., Zhang,S., Beejadhursing,R., Sun,Y., Qin,Y., Chen,S. and
            Li,W.
  TITLE     Extracellular vesicles derived from hypoxic HTR-8/SVneo trophoblast
            inhibit endothelial cell functions through the miR-150-3p /CHPF
            pathway
  JOURNAL   Placenta 138, 21-32 (2023)
   PUBMED   37156185
  REMARK    GeneRIF: Extracellular vesicles derived from hypoxic HTR-8/SVneo
            trophoblast inhibit endothelial cell functions through the
            miR-150-3p /CHPF pathway.
REFERENCE   6  (bases 1 to 2628)
  AUTHORS   Colland,F., Jacq,X., Trouplin,V., Mougin,C., Groizeleau,C.,
            Hamburger,A., Meil,A., Wojcik,J., Legrain,P. and Gauthier,J.M.
  TITLE     Functional proteomics mapping of a human signaling pathway
  JOURNAL   Genome Res 14 (7), 1324-1332 (2004)
   PUBMED   15231748
REFERENCE   7  (bases 1 to 2628)
  AUTHORS   Lehner,B. and Sanderson,C.M.
  TITLE     A protein interaction framework for human mRNA degradation
  JOURNAL   Genome Res 14 (7), 1315-1323 (2004)
   PUBMED   15231747
REFERENCE   8  (bases 1 to 2628)
  AUTHORS   Clark,H.F., Gurney,A.L., Abaya,E., Baker,K., Baldwin,D., Brush,J.,
            Chen,J., Chow,B., Chui,C., Crowley,C., Currell,B., Deuel,B.,
            Dowd,P., Eaton,D., Foster,J., Grimaldi,C., Gu,Q., Hass,P.E.,
            Heldens,S., Huang,A., Kim,H.S., Klimowski,L., Jin,Y., Johnson,S.,
            Lee,J., Lewis,L., Liao,D., Mark,M., Robbie,E., Sanchez,C.,
            Schoenfeld,J., Seshagiri,S., Simmons,L., Singh,J., Smith,V.,
            Stinson,J., Vagts,A., Vandlen,R., Watanabe,C., Wieand,D., Woods,K.,
            Xie,M.H., Yansura,D., Yi,S., Yu,G., Yuan,J., Zhang,M., Zhang,Z.,
            Goddard,A., Wood,W.I., Godowski,P. and Gray,A.
  TITLE     The secreted protein discovery initiative (SPDI), a large-scale
            effort to identify novel human secreted and transmembrane proteins:
            a bioinformatics assessment
  JOURNAL   Genome Res 13 (10), 2265-2270 (2003)
   PUBMED   12975309
  REMARK    Erratum:[Genome Res. 2003 Dec;13(12):2759]
REFERENCE   9  (bases 1 to 2628)
  AUTHORS   Yada,T., Gotoh,M., Sato,T., Shionyu,M., Go,M., Kaseyama,H.,
            Iwasaki,H., Kikuchi,N., Kwon,Y.D., Togayachi,A., Kudo,T.,
            Watanabe,H., Narimatsu,H. and Kimata,K.
  TITLE     Chondroitin sulfate synthase-2. Molecular cloning and
            characterization of a novel human glycosyltransferase homologous to
            chondroitin sulfate glucuronyltransferase, which has dual enzymatic
            activities
  JOURNAL   J Biol Chem 278 (32), 30235-30247 (2003)
   PUBMED   12761225
REFERENCE   10 (bases 1 to 2628)
  AUTHORS   Kitagawa,H., Izumikawa,T., Uyama,T. and Sugahara,K.
  TITLE     Molecular cloning of a chondroitin polymerizing factor that
            cooperates with chondroitin synthase for chondroitin polymerization
  JOURNAL   J Biol Chem 278 (26), 23666-23671 (2003)
   PUBMED   12716890
  REMARK    GeneRIF: chondroitin polymerizing activity requires concomitant
            expression of a ChPF with ChSy; coexpression of the ChPF and ChSy
            yielded markedly augmented glycosyltransferase activities, whereas
            simple mixing of the two separately expressed proteins did not.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC009955.4.
            
            On Aug 14, 2020 this sequence version replaced NM_001195731.1.
            
            Transcript Variant: This variant (2) contains an alternate 5'
            terminal exon compared to variant 1, resulting in translation
            initiation from an in-frame downstream AUG, and an isoform (2) with
            a shorter N-terminus compared to isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK302124.1, SRR14038192.1823090.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA2148874
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-185               AC009955.4         13802-13986         c
            186-759             AC009955.4         12505-13078         c
            760-939             AC009955.4         11835-12014         c
            940-2628            AC009955.4         9843-11531          c
FEATURES             Location/Qualifiers
     source          1..2628
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q35"
     gene            1..2628
                     /gene="CHPF"
                     /gene_synonym="CHPF1; CHSY2; CSS2"
                     /note="chondroitin polymerizing factor"
                     /db_xref="GeneID:79586"
                     /db_xref="HGNC:HGNC:24291"
                     /db_xref="MIM:610405"
     exon            1..185
                     /gene="CHPF"
                     /gene_synonym="CHPF1; CHSY2; CSS2"
                     /inference="alignment:Splign:2.1.0"
     exon            186..759
                     /gene="CHPF"
                     /gene_synonym="CHPF1; CHSY2; CSS2"
                     /inference="alignment:Splign:2.1.0"
     CDS             358..2199
                     /gene="CHPF"
                     /gene_synonym="CHPF1; CHSY2; CSS2"
                     /EC_number="2.4.1.175"
                     /EC_number="2.4.1.226"
                     /note="isoform 2 is encoded by transcript variant 2;
                     chondroitin sulfate synthase 2;
                     N-acetylgalactosaminyltransferase 2; chondroitin
                     glucuronyltransferase 2;
                     N-acetylgalactosaminyl-proteoglycan
                     3-beta-glucuronosyltransferase II;
                     glucuronosyl-N-acetylgalactosaminyl-proteoglycan
                     4-beta-N-acetylgalactosaminyltransferase II"
                     /codon_start=1
                     /product="chondroitin sulfate synthase 2 isoform 2"
                     /protein_id="NP_001182660.2"
                     /db_xref="CCDS:CCDS56169.1"
                     /db_xref="GeneID:79586"
                     /db_xref="HGNC:HGNC:24291"
                     /db_xref="MIM:610405"
                     /translation="MAVVTLGEERPIGHLHLALRHLLEQHGDDFDWFFLVPDTTYTEA
                     HGLARLTGHLSLASAAHLYLGRPQDFIGGEPTPGRYCHGGFGVLLSRMLLQQLRPHLE
                     GCRNDIVSARPDEWLGRCILDATGVGCTGDHEGVHYSHLELSPGEPVQEGDPHFRSAL
                     TAHPVRDPVHMYQLHKAFARAELERTYQEIQELQWEIQNTSHLAVDGDQAAAWPVGIP
                     APSRPASRFEVLRWDYFTEQHAFSCADGSPRCPLRGADRADVADVLGTALEELNRRYH
                     PALRLQKQQLVNGYRRFDPARGMEYTLDLQLEALTPQGGRRPLTRRVQLLRPLSRVEI
                     LPVPYVTEASRLTVLLPLAAAERDLAPGFLEAFATAALEPGDAAAALTLLLLYEPRQA
                     QRVAHADVFAPVKAHVAELERRFPGARVPWLSVQTAAPSPLRLMDLLSKKHPLDTLFL
                     LAGPDTVLTPDFLNRCRMHAISGWQAFFPMHFQAFHPAVAPPQGPGPPELGRDTGRFD
                     RQAASEACFYNSDYVAARGRLAAASEQEEELLESLDVYELFLHFSSLHVLRAVEPALL
                     QRYRAQTCSARLSEDLYHRCLQSVLEGLGSRTQLAMLLFEQEQGNST"
     exon            760..939
                     /gene="CHPF"
                     /gene_synonym="CHPF1; CHSY2; CSS2"
                     /inference="alignment:Splign:2.1.0"
     exon            940..2628
                     /gene="CHPF"
                     /gene_synonym="CHPF1; CHSY2; CSS2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2608..2613
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CHPF"
                     /gene_synonym="CHPF1; CHSY2; CSS2"
                     /note="hexamer: AATAAA"
     polyA_site      2628
                     /gene="CHPF"
                     /gene_synonym="CHPF1; CHSY2; CSS2"
                     /note="major polyA site"
ORIGIN      
        1 aacttccgat ggccctgcct tcccgagagc cgtggggcca gtccgagact ttctctgggc
       61 cctatcccgg cctgggctga acttactgga ctgtgtccta aaccggctcg acccgatggc
      121 gccccagaga tcctactagc tccctggctg ggctgctggt gttagagaaa tgacttatat
      181 atagggaccc gctacatcag cacggagctg ggcatcaggc agaggctgct ggtggcggtg
      241 ctgacctctc agaccacgct gcccacgctg ggcgtggccg tgaaccgcac gctggggcac
      301 cggctggagc gtgtggtgtt cctgacgggc gcacggggcc gccgggcccc acctggcatg
      361 gcagtggtga cgctaggcga ggagcgaccc attggacacc tgcacctggc gctgcgccac
      421 ctgctggagc agcacggcga cgactttgac tggttcttcc tggtgcctga caccacctac
      481 accgaggcgc acggcctggc acgcctaact ggccacctca gcctggcctc cgccgcccac
      541 ctgtacctgg gccggcccca ggacttcatc ggcggagagc ccacccccgg ccgctactgc
      601 cacggaggct ttggggtgct gctgtcgcgc atgctgctgc aacaactgcg cccccacctg
      661 gaaggctgcc gcaacgacat cgtcagtgcg cgccctgacg agtggctggg tcgctgcatt
      721 ctcgatgcca ccggggtggg ctgcactggt gaccacgagg gggtgcacta tagccatctg
      781 gagctgagcc ctggggagcc agtgcaggag ggggaccctc atttccgaag tgccctgaca
      841 gcccaccctg tgcgtgaccc tgtgcacatg taccagctgc acaaagcttt cgcccgagct
      901 gaactggaac gcacgtacca ggagatccag gagttacagt gggagatcca gaataccagc
      961 catctggccg ttgatgggga ccaggcagct gcttggcccg tgggtattcc agcaccatcc
     1021 cgcccggcct cccgctttga ggtgctgcgc tgggactact tcacggagca gcacgctttc
     1081 tcctgcgccg atggctcacc ccgctgccca ctgcgtgggg ctgaccgggc tgatgtggcc
     1141 gatgttctgg ggacagctct agaggagctg aaccgccgct accacccggc cttgcggctc
     1201 cagaagcagc agctggtgaa tggctaccga cgctttgatc cggcccgggg tatggaatac
     1261 acgctggact tgcagctgga ggcactgacc ccccagggag gccgccggcc cctcactcgc
     1321 cgagtgcagc tgctccggcc gctgagccgc gtggagatct tgcctgtgcc ctatgtcact
     1381 gaggcctcac gtctcactgt gctgctgcct ctagctgcgg ctgagcgtga cctggcccct
     1441 ggcttcttgg aggcctttgc cactgcagca ctggagcctg gtgatgctgc ggcagccctg
     1501 accctgctgc tactgtatga gccgcgccag gcccagcgcg tggcccatgc agatgtcttc
     1561 gcacctgtca aggcccacgt ggcagagctg gagcggcgtt tccccggtgc ccgggtgcca
     1621 tggctcagtg tgcagacagc cgcaccctca ccactgcgcc tcatggatct actctccaag
     1681 aagcacccgc tggacacact gttcctgctg gccgggccag acacggtgct cacgcctgac
     1741 ttcctgaacc gctgccgcat gcatgccatc tccggctggc aggccttctt tcccatgcat
     1801 ttccaagcct tccacccagc tgtggcccca ccacaagggc ctgggccccc agagctgggc
     1861 cgtgacactg gccgctttga tcgccaggca gccagcgagg cctgcttcta caactccgac
     1921 tatgtggcag cccgtgggcg cctggcggca gcctcagaac aagaagagga gctgctggag
     1981 agcctggatg tgtacgagct gttcctccac ttctccagtc tgcatgtgct gcgggcggtg
     2041 gagccggcgc tgctgcagcg ctaccgggcc cagacgtgca gcgcgaggct cagtgaggac
     2101 ctgtaccacc gctgcctcca gagcgtgctt gagggcctcg gctcccgaac ccagctggcc
     2161 atgctactct ttgaacagga gcagggcaac agcacctgac cccaccctgt ccccgtgggc
     2221 cgtggcatgg ccacacccca ccccacttct cccccaaaac cagagccacc tgccagcctc
     2281 gctgggcagg gctggccgta gccagacccc aagctggccc actggtcccc tctctggctc
     2341 tgtgggtccc tgggctctgg acaagcactg ggggacgtgc ccccagagcc acccacttct
     2401 catcccaaac ccagtttccc tgccccctga cgctgctgat tcgggctgtg gcctccacgt
     2461 atttatgcag tacagtctgc ctgacgccag ccctgcctct gggccctggg ggctgggctg
     2521 tagaagagtt gttggggaag gagggagctg aggagggggc atctcccaac ttctcccttt
     2581 tggaccctgc cgaagctccc tgcctttaat aaactggcca agtgtgga
//
