LOCUS       NM_004067               3167 bp    mRNA    linear   PRI 09-JUN-2024
DEFINITION  Homo sapiens chimerin 2 (CHN2), transcript variant 2, mRNA.
ACCESSION   NM_004067
VERSION     NM_004067.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3167)
  AUTHORS   Lee,J., Kim,J., Zinia,S.S., Park,J., Won,S. and Kim,W.J.
  TITLE     Prenatal phthalate exposure and cord blood DNA methylation
  JOURNAL   Sci Rep 13 (1), 7046 (2023)
   PUBMED   37120575
  REMARK    GeneRIF: Prenatal phthalate exposure and cord blood DNA
            methylation.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3167)
  AUTHORS   Zhang,Y., Shen,W.J., Qiu,S., Yang,P., Dempsey,G., Zhao,L., Zhou,Q.,
            Hao,X., Dong,D., Stahl,A., Kraemer,F.B., Leung,L.L. and Morser,J.
  TITLE     Chemerin regulates formation and function of brown adipose tissue:
            Ablation results in increased insulin resistance with high fat
            challenge and aging
  JOURNAL   FASEB J 35 (7), e21687 (2021)
   PUBMED   34089273
  REMARK    GeneRIF: Chemerin regulates formation and function of brown adipose
            tissue: Ablation results in increased insulin resistance with high
            fat challenge and aging.
REFERENCE   3  (bases 1 to 3167)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 3167)
  AUTHORS   Ju,C., Fiori,L.M., Belzeaux,R., Theroux,J.F., Chen,G.G.,
            Aouabed,Z., Blier,P., Farzan,F., Frey,B.N., Giacobbe,P., Lam,R.W.,
            Leri,F., MacQueen,G.M., Milev,R., Muller,D.J., Parikh,S.V.,
            Rotzinger,S., Soares,C.N., Uher,R., Li,Q., Foster,J.A.,
            Kennedy,S.H. and Turecki,G.
  TITLE     Integrated genome-wide methylation and expression analyses reveal
            functional predictors of response to antidepressants
  JOURNAL   Transl Psychiatry 9 (1), 254 (2019)
   PUBMED   31594917
  REMARK    GeneRIF: The findings indicate that differential methylation at CpG
            sites upstream of the CHN2 and JAK2 TSS regions are possible
            peripheral predictors of antidepressant treatment response.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3167)
  AUTHORS   Xu,C.H., Yang,Y., Wang,Y.C., Yan,J. and Qian,L.H.
  TITLE     Prognostic significance of serum chemerin levels in patients with
            non-small cell lung cancer
  JOURNAL   Oncotarget 8 (14), 22483-22489 (2017)
   PUBMED   28160556
  REMARK    GeneRIF: this study shows that serum chemerin is an independent
            risk factor of prognosis of non-small cell lung cancer patients
REFERENCE   6  (bases 1 to 3167)
  AUTHORS   Caloca,M.J., Wang,H., Delemos,A., Wang,S. and Kazanietz,M.G.
  TITLE     Phorbol esters and related analogs regulate the subcellular
            localization of beta 2-chimaerin, a non-protein kinase C phorbol
            ester receptor
  JOURNAL   J Biol Chem 276 (21), 18303-18312 (2001)
   PUBMED   11278894
REFERENCE   7  (bases 1 to 3167)
  AUTHORS   Caloca,M.J., Garcia-Bermejo,M.L., Blumberg,P.M., Lewin,N.E.,
            Kremmer,E., Mischak,H., Wang,S., Nacro,K., Bienfait,B.,
            Marquez,V.E. and Kazanietz,M.G.
  TITLE     beta2-chimaerin is a novel target for diacylglycerol: binding
            properties and changes in subcellular localization mediated by
            ligand binding to its C1 domain
  JOURNAL   Proc Natl Acad Sci U S A 96 (21), 11854-11859 (1999)
   PUBMED   10518540
REFERENCE   8  (bases 1 to 3167)
  AUTHORS   Caloca,M.J., Fernandez,N., Lewin,N.E., Ching,D., Modali,R.,
            Blumberg,P.M. and Kazanietz,M.G.
  TITLE     Beta2-chimaerin is a high affinity receptor for the phorbol ester
            tumor promoters
  JOURNAL   J Biol Chem 272 (42), 26488-26496 (1997)
   PUBMED   9334226
REFERENCE   9  (bases 1 to 3167)
  AUTHORS   Yuan,S., Miller,D.W., Barnett,G.H., Hahn,J.F. and Williams,B.R.
  TITLE     Identification and characterization of human beta 2-chimaerin:
            association with malignant transformation in astrocytoma
  JOURNAL   Cancer Res 55 (15), 3456-3461 (1995)
   PUBMED   7614486
REFERENCE   10 (bases 1 to 3167)
  AUTHORS   Leung,T., How,B.E., Manser,E. and Lim,L.
  TITLE     Cerebellar beta 2-chimaerin, a GTPase-activating protein for p21
            ras-related rac is specifically expressed in granule cells and has
            a unique N-terminal SH2 domain
  JOURNAL   J Biol Chem 269 (17), 12888-12892 (1994)
   PUBMED   8175705
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC004593.1, AC004417.1 and
            AC007255.4.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 30, 2019 this sequence version replaced NM_004067.3.
            
            Summary: This gene encodes a guanosine triphosphate
            (GTP)-metabolizing protein that contains a
            phorbol-ester/diacylglycerol (DAG)-type zinc finger, a Rho-GAP
            domain, and an SH2 domain. The encoded protein translocates from
            the cytosol to the Golgi apparatus membrane upon binding by
            diacylglycerol (DAG). Activity of this protein is important in cell
            proliferation and migration, and expression changes in this gene
            have been detected in cancers. A mutation in this gene has also
            been associated with schizophrenia in men. Alternative transcript
            splicing and the use of alternative promoters results in multiple
            transcript variants. [provided by RefSeq, May 2014].
            
            Transcript Variant: This variant (2) differs in the 5' UTR and
            contains multiple additional 5' coding exons, compared to variant
            1. It represents use of an alternate promoter and initiates
            translation from an alternate start codon. The encoded isoform (2)
            has a distinct N-terminus and is longer than isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR11853565.25921.1,
                                           SRR11853560.25898.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000222792.11/ ENSP00000222792.7
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-216               AC004593.1         10682-10897
            217-255             AC004417.1         20511-20549
            256-311             AC004417.1         33818-33873
            312-343             AC004417.1         59565-59596
            344-457             AC004417.1         64259-64372
            458-743             AC004417.1         66429-66714
            744-821             AC007255.4         18851-18928
            822-906             AC007255.4         34524-34608
            907-1080            AC007255.4         38439-38612
            1081-1158           AC007255.4         43316-43393
            1159-1296           AC007255.4         45800-45937
            1297-1402           AC007255.4         47873-47978
            1403-3167           AC007255.4         51136-52900
FEATURES             Location/Qualifiers
     source          1..3167
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7p14.3"
     gene            1..3167
                     /gene="CHN2"
                     /gene_synonym="ARHGAP3; BCH; CHN2-3; RHOGAP3"
                     /note="chimerin 2"
                     /db_xref="GeneID:1124"
                     /db_xref="HGNC:HGNC:1944"
                     /db_xref="MIM:602857"
     exon            1..216
                     /gene="CHN2"
                     /gene_synonym="ARHGAP3; BCH; CHN2-3; RHOGAP3"
                     /inference="alignment:Splign:2.1.0"
     CDS             168..1574
                     /gene="CHN2"
                     /gene_synonym="ARHGAP3; BCH; CHN2-3; RHOGAP3"
                     /note="isoform 2 is encoded by transcript variant 2;
                     rho-GTPase-activating protein 3; beta3-chimaerin; chimerin
                     (chimaerin) 2; beta-chimerin; beta-chimaerin; chimerin,
                     beta-2; chimerin, testis-specific"
                     /codon_start=1
                     /product="beta-chimaerin isoform 2"
                     /protein_id="NP_004058.1"
                     /db_xref="CCDS:CCDS5420.1"
                     /db_xref="GeneID:1124"
                     /db_xref="HGNC:HGNC:1944"
                     /db_xref="MIM:602857"
                     /translation="MAASSNSSLSGSSVSSDAEEYQPPIWKSYLYQLQQEAPRPKRII
                     CPREVENRPKYYGREFHGIISREQADELLGGVEGAYILRESQRQPGCYTLALRFGNQT
                     LNYRLFHDGKHFVGEKRFESIHDLVTDGLITLYIETKAAEYISKMTTNPIYEHIGYAT
                     LLREKVSRRLSRSKNEPRKTNVTHEEHTAVEKISSLVRRAALTHNDNHFNYEKTHNFK
                     VHTFRGPHWCEYCANFMWGLIAQGVRCSDCGLNVHKQCSKHVPNDCQPDLKRIKKVYC
                     CDLTTLVKAHNTQRPMVVDICIREIEARGLKSEGLYRVSGFTEHIEDVKMAFDRDGEK
                     ADISANVYPDINIITGALKLYFRDLPIPVITYDTYSKFIDAAKISNADERLEAVHEVL
                     MLLPPAHYETLRYLMIHLKKVTMNEKDNFMNAENLGIVFGPTLMRPPEDSTLTTLHDM
                     RYQKLIVQILIENEDVLF"
     exon            217..255
                     /gene="CHN2"
                     /gene_synonym="ARHGAP3; BCH; CHN2-3; RHOGAP3"
                     /inference="alignment:Splign:2.1.0"
     exon            256..311
                     /gene="CHN2"
                     /gene_synonym="ARHGAP3; BCH; CHN2-3; RHOGAP3"
                     /inference="alignment:Splign:2.1.0"
     exon            312..343
                     /gene="CHN2"
                     /gene_synonym="ARHGAP3; BCH; CHN2-3; RHOGAP3"
                     /inference="alignment:Splign:2.1.0"
     exon            344..457
                     /gene="CHN2"
                     /gene_synonym="ARHGAP3; BCH; CHN2-3; RHOGAP3"
                     /inference="alignment:Splign:2.1.0"
     exon            458..743
                     /gene="CHN2"
                     /gene_synonym="ARHGAP3; BCH; CHN2-3; RHOGAP3"
                     /inference="alignment:Splign:2.1.0"
     exon            744..821
                     /gene="CHN2"
                     /gene_synonym="ARHGAP3; BCH; CHN2-3; RHOGAP3"
                     /inference="alignment:Splign:2.1.0"
     exon            822..906
                     /gene="CHN2"
                     /gene_synonym="ARHGAP3; BCH; CHN2-3; RHOGAP3"
                     /inference="alignment:Splign:2.1.0"
     exon            907..1080
                     /gene="CHN2"
                     /gene_synonym="ARHGAP3; BCH; CHN2-3; RHOGAP3"
                     /inference="alignment:Splign:2.1.0"
     exon            1081..1158
                     /gene="CHN2"
                     /gene_synonym="ARHGAP3; BCH; CHN2-3; RHOGAP3"
                     /inference="alignment:Splign:2.1.0"
     exon            1159..1296
                     /gene="CHN2"
                     /gene_synonym="ARHGAP3; BCH; CHN2-3; RHOGAP3"
                     /inference="alignment:Splign:2.1.0"
     exon            1297..1402
                     /gene="CHN2"
                     /gene_synonym="ARHGAP3; BCH; CHN2-3; RHOGAP3"
                     /inference="alignment:Splign:2.1.0"
     exon            1403..3167
                     /gene="CHN2"
                     /gene_synonym="ARHGAP3; BCH; CHN2-3; RHOGAP3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3147..3152
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CHN2"
                     /gene_synonym="ARHGAP3; BCH; CHN2-3; RHOGAP3"
                     /note="hexamer: ATTAAA"
     polyA_site      3167
                     /gene="CHN2"
                     /gene_synonym="ARHGAP3; BCH; CHN2-3; RHOGAP3"
                     /note="major polyA site"
ORIGIN      
        1 ggtggagcag gaagtgcagg cagagtccgg aggctggtgc tttctgcgcg tccccaggac
       61 tttgccatgg gctgggggcc gcggaggctg cgagcggccg ggcgagggca gcggcggcgg
      121 cgtccgcacc ggggctgagc gagcagcgac gcgaggggcg cgcggagatg gcagcgtcca
      181 gcaactccag cctgtccggc tcgtcggtgt cctccgatgc tgaagaatac cagcctccta
      241 tatggaaatc atacttatat cagttacagc aagaggcacc tcgtcccaag agaatcattt
      301 gtcctcggga ggtggaaaac agaccaaaat attatggaag agagtttcat gggatcatct
      361 ctcgggagca ggcggatgag cttcttggag gcgtggaggg tgcctacatc cttagagaaa
      421 gccagcggca accaggatgc tacacgctgg ctctcaggtt tggaaaccag accttaaact
      481 acaggctctt ccacgacggg aaacactttg tgggtgagaa gaggtttgag tcgattcatg
      541 atctggtgac agatggcttg ataacactgt acatagaaac aaaagctgcc gagtacattt
      601 caaaaatgac aactaacccc atctatgaac acattggata tgccacccta ctcagagaaa
      661 aagtatccag aaggctgagc aggtctaaaa atgaaccaag aaaaacaaac gtcacacatg
      721 aagaacacac agcggtggaa aagatctcct ccctggttcg aagggctgcc ctcacacaca
      781 acgacaacca cttcaattat gagaagacac acaactttaa ggtccacacg ttccgaggcc
      841 cacactggtg tgaatattgt gccaatttca tgtgggggct catcgcccaa ggggtccggt
      901 gctcagactg tggattgaac gtacacaaac agtgttccaa gcacgttccc aatgactgcc
      961 aacctgatct caagaggatc aagaaagtgt actgttgtga cctcacaaca cttgtgaagg
     1021 ctcacaacac tcagagaccc atggtggtag acatatgcat tcgggaaatt gaagcaagag
     1081 gattaaaatc ggaaggcctt tacagagtct ctgggttcac tgaacacatt gaagatgtca
     1141 aaatggcatt tgacagagat ggtgaaaagg ccgatatatc tgccaatgtc tatccagaca
     1201 taaacatcat cactggagcc cttaaactgt atttcagaga cttacccatc cctgtcatca
     1261 catatgatac ctattccaaa tttatagatg cagcaaaaat ctccaatgca gatgagaggc
     1321 tggaagccgt ccatgaagtg ctgatgctgc tgcctcctgc ccactatgaa accctccggt
     1381 acctaatgat ccacctcaaa aaggttacta tgaatgaaaa agacaatttc atgaatgcag
     1441 aaaatctggg gatcgtgttt gggcccactc tgatgaggcc ccctgaggac agcaccctga
     1501 ccaccctgca tgatatgcgg taccaaaagc tgattgtgca gattttaata gaaaacgaag
     1561 acgttttatt ctaatccatc agggaaatga gctgaatggc cccagcacca tccaagttga
     1621 cacagctaaa ggaataaaaa catttcttac cacttgattt gttttccaag caagtgctag
     1681 aatttcctgg actgcagagg atcgctgagt ggggtactgt gtctcataga catgcgccac
     1741 ctccacgtga gaacaagggt gaaggtgagg gaagcccctc accttgggtc ttttgctgtg
     1801 cctcctatgt atgtctggtt tgctggaaga gtgattaata catctttaat ttattaaaaa
     1861 acaatgtaga cctttaaact tcagtcttat tggtaataaa agggaactta attcatacag
     1921 gtacttgata cagttataca ttttccactt acaaaaagaa gacaattctg ttaaatgaaa
     1981 cgtgtatcgt aaaatgtatt tttattttac ccacgagaat gttgttattt ttagcaaata
     2041 gaactcaatg cagtgcattg gttattaccc tgtgtacctt gtccctcatt ttgctgtgac
     2101 accctgaaaa agctgaccac aaatgcagta ttatcttgac atacctctgt cctcctcagt
     2161 gctttttaat gaaatttcac ctgccccttg tgttcctgct ttggatagtt tttgctctta
     2221 agcattggca ttcttctatt gtatggtatg tatttatagc aattgtagga tggtctgaaa
     2281 tgtccacaca ctttctatgc atacaaattt tgtgaattcc caaagtatgc tttggaattg
     2341 gtcatagtct tttcgtatca tgagtgtgta tcccagaaga tacagaatgc ggtcatttta
     2401 cctgaaatta tttgagaaca ctggatgtat ctggtctgtg taaaaagtta gcacttaccc
     2461 tcttttcttc ctggccaagc cctctgaatt catctgttaa tatcaaattg tatttttgcc
     2521 acatttctgt attggtgaaa agcgaatctg cagaataact gtcaacgttt ttcattcttg
     2581 ttttacaaac ctatttttta aagtgagaat aaggttggtt tttaccaaat atttgttcct
     2641 ccttgacaaa agtagctctg tgtggataat atgattttat tactacagtt ccagtcactt
     2701 ggttatattt atcttagcat gagcctttca caccaagatt tctatatttt gtaacatagg
     2761 tgaaatattt aaaacatcaa aaactgtaaa tctagatttt taaaaaatcc aactgcaatg
     2821 tcttttcctt tgattgagat gatttgtgta aactcaccag tcttgctttg gagtgagcag
     2881 aagagaatga ctattttacg tggagcatca ttgtgtgact gttgacctgg acagtcccaa
     2941 gggctatgca gatggactcc attggcacag ggaggtttga cctcttccct gctattatcc
     3001 ctcctcccaa ctgtacagat ttgtttgttg tagtttatgt acttcttgat actgtcaaaa
     3061 aattatctgg aaatggtttt attttgtgaa gtgaacaata ctttgtaatt tatgatagtg
     3121 taaatggaag gcaaagcatc acatgtatta aattcttctg tctatta
//
