LOCUS       NM_001293077            2434 bp    mRNA    linear   PRI 10-JUN-2024
DEFINITION  Homo sapiens chimerin 2 (CHN2), transcript variant 10, mRNA.
ACCESSION   NM_001293077
VERSION     NM_001293077.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Lee,J., Kim,J., Zinia,S.S., Park,J., Won,S. and Kim,W.J.
  TITLE     Prenatal phthalate exposure and cord blood DNA methylation
  JOURNAL   Sci Rep 13 (1), 7046 (2023)
   PUBMED   37120575
  REMARK    GeneRIF: Prenatal phthalate exposure and cord blood DNA
            methylation.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2434)
  AUTHORS   Zhang,Y., Shen,W.J., Qiu,S., Yang,P., Dempsey,G., Zhao,L., Zhou,Q.,
            Hao,X., Dong,D., Stahl,A., Kraemer,F.B., Leung,L.L. and Morser,J.
  TITLE     Chemerin regulates formation and function of brown adipose tissue:
            Ablation results in increased insulin resistance with high fat
            challenge and aging
  JOURNAL   FASEB J 35 (7), e21687 (2021)
   PUBMED   34089273
  REMARK    GeneRIF: Chemerin regulates formation and function of brown adipose
            tissue: Ablation results in increased insulin resistance with high
            fat challenge and aging.
REFERENCE   3  (bases 1 to 2434)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 2434)
  AUTHORS   Ju,C., Fiori,L.M., Belzeaux,R., Theroux,J.F., Chen,G.G.,
            Aouabed,Z., Blier,P., Farzan,F., Frey,B.N., Giacobbe,P., Lam,R.W.,
            Leri,F., MacQueen,G.M., Milev,R., Muller,D.J., Parikh,S.V.,
            Rotzinger,S., Soares,C.N., Uher,R., Li,Q., Foster,J.A.,
            Kennedy,S.H. and Turecki,G.
  TITLE     Integrated genome-wide methylation and expression analyses reveal
            functional predictors of response to antidepressants
  JOURNAL   Transl Psychiatry 9 (1), 254 (2019)
   PUBMED   31594917
  REMARK    GeneRIF: The findings indicate that differential methylation at CpG
            sites upstream of the CHN2 and JAK2 TSS regions are possible
            peripheral predictors of antidepressant treatment response.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2434)
  AUTHORS   Xu,C.H., Yang,Y., Wang,Y.C., Yan,J. and Qian,L.H.
  TITLE     Prognostic significance of serum chemerin levels in patients with
            non-small cell lung cancer
  JOURNAL   Oncotarget 8 (14), 22483-22489 (2017)
   PUBMED   28160556
  REMARK    GeneRIF: this study shows that serum chemerin is an independent
            risk factor of prognosis of non-small cell lung cancer patients
REFERENCE   6  (bases 1 to 2434)
  AUTHORS   Caloca,M.J., Wang,H., Delemos,A., Wang,S. and Kazanietz,M.G.
  TITLE     Phorbol esters and related analogs regulate the subcellular
            localization of beta 2-chimaerin, a non-protein kinase C phorbol
            ester receptor
  JOURNAL   J Biol Chem 276 (21), 18303-18312 (2001)
   PUBMED   11278894
REFERENCE   7  (bases 1 to 2434)
  AUTHORS   Caloca,M.J., Garcia-Bermejo,M.L., Blumberg,P.M., Lewin,N.E.,
            Kremmer,E., Mischak,H., Wang,S., Nacro,K., Bienfait,B.,
            Marquez,V.E. and Kazanietz,M.G.
  TITLE     beta2-chimaerin is a novel target for diacylglycerol: binding
            properties and changes in subcellular localization mediated by
            ligand binding to its C1 domain
  JOURNAL   Proc Natl Acad Sci U S A 96 (21), 11854-11859 (1999)
   PUBMED   10518540
REFERENCE   8  (bases 1 to 2434)
  AUTHORS   Caloca,M.J., Fernandez,N., Lewin,N.E., Ching,D., Modali,R.,
            Blumberg,P.M. and Kazanietz,M.G.
  TITLE     Beta2-chimaerin is a high affinity receptor for the phorbol ester
            tumor promoters
  JOURNAL   J Biol Chem 272 (42), 26488-26496 (1997)
   PUBMED   9334226
REFERENCE   9  (bases 1 to 2434)
  AUTHORS   Yuan,S., Miller,D.W., Barnett,G.H., Hahn,J.F. and Williams,B.R.
  TITLE     Identification and characterization of human beta 2-chimaerin:
            association with malignant transformation in astrocytoma
  JOURNAL   Cancer Res 55 (15), 3456-3461 (1995)
   PUBMED   7614486
REFERENCE   10 (bases 1 to 2434)
  AUTHORS   Leung,T., How,B.E., Manser,E. and Lim,L.
  TITLE     Cerebellar beta 2-chimaerin, a GTPase-activating protein for p21
            ras-related rac is specifically expressed in granule cells and has
            a unique N-terminal SH2 domain
  JOURNAL   J Biol Chem 269 (17), 12888-12892 (1994)
   PUBMED   8175705
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC007255.4.
            
            On Aug 14, 2020 this sequence version replaced NM_001293077.1.
            
            Summary: This gene encodes a guanosine triphosphate
            (GTP)-metabolizing protein that contains a
            phorbol-ester/diacylglycerol (DAG)-type zinc finger, a Rho-GAP
            domain, and an SH2 domain. The encoded protein translocates from
            the cytosol to the Golgi apparatus membrane upon binding by
            diacylglycerol (DAG). Activity of this protein is important in cell
            proliferation and migration, and expression changes in this gene
            have been detected in cancers. A mutation in this gene has also
            been associated with schizophrenia in men. Alternative transcript
            splicing and the use of alternative promoters results in multiple
            transcript variants. [provided by RefSeq, May 2014].
            
            Transcript Variant: This variant (10, also known as B1-CHNdel
            ex10-11) lacks two alternate in-frame exons, compared to variant 1.
            The encoded isoform (10) is shorter than isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: EU732756.1 [ECO:0000332]
            RNAseq introns              :: partial sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0000350]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-304               AC007255.4         18625-18928
            305-389             AC007255.4         34524-34608
            390-563             AC007255.4         38439-38612
            564-669             AC007255.4         47873-47978
            670-2434            AC007255.4         51136-52900
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7p14.3"
     gene            1..2434
                     /gene="CHN2"
                     /gene_synonym="ARHGAP3; BCH; CHN2-3; RHOGAP3"
                     /note="chimerin 2"
                     /db_xref="GeneID:1124"
                     /db_xref="HGNC:HGNC:1944"
                     /db_xref="MIM:602857"
     exon            1..304
                     /gene="CHN2"
                     /gene_synonym="ARHGAP3; BCH; CHN2-3; RHOGAP3"
                     /inference="alignment:Splign:2.1.0"
     CDS             59..841
                     /gene="CHN2"
                     /gene_synonym="ARHGAP3; BCH; CHN2-3; RHOGAP3"
                     /note="isoform 10 is encoded by transcript variant 10;
                     rho-GTPase-activating protein 3; beta3-chimaerin; chimerin
                     (chimaerin) 2; beta-chimerin; beta-chimaerin; chimerin,
                     beta-2; chimerin, testis-specific"
                     /codon_start=1
                     /product="beta-chimaerin isoform 10"
                     /protein_id="NP_001280006.1"
                     /db_xref="CCDS:CCDS78218.1"
                     /db_xref="GeneID:1124"
                     /db_xref="HGNC:HGNC:1944"
                     /db_xref="MIM:602857"
                     /translation="MFSEELWLENEKKCAVVRKSKQGRKRQELLAVAFGVKVGVKGGF
                     LWPPLKLFACSQISSLVRRAALTHNDNHFNYEKTHNFKVHTFRGPHWCEYCANFMWGL
                     IAQGVRCSDCGLNVHKQCSKHVPNDCQPDLKRIKKVYCCDLTTLVKAHNTQRPMVVDI
                     CIREIEAREISNADERLEAVHEVLMLLPPAHYETLRYLMIHLKKVTMNEKDNFMNAEN
                     LGIVFGPTLMRPPEDSTLTTLHDMRYQKLIVQILIENEDVLF"
     exon            305..389
                     /gene="CHN2"
                     /gene_synonym="ARHGAP3; BCH; CHN2-3; RHOGAP3"
                     /inference="alignment:Splign:2.1.0"
     exon            390..563
                     /gene="CHN2"
                     /gene_synonym="ARHGAP3; BCH; CHN2-3; RHOGAP3"
                     /inference="alignment:Splign:2.1.0"
     exon            564..669
                     /gene="CHN2"
                     /gene_synonym="ARHGAP3; BCH; CHN2-3; RHOGAP3"
                     /inference="alignment:Splign:2.1.0"
     exon            670..2434
                     /gene="CHN2"
                     /gene_synonym="ARHGAP3; BCH; CHN2-3; RHOGAP3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2414..2419
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CHN2"
                     /gene_synonym="ARHGAP3; BCH; CHN2-3; RHOGAP3"
                     /note="hexamer: ATTAAA"
     polyA_site      2434
                     /gene="CHN2"
                     /gene_synonym="ARHGAP3; BCH; CHN2-3; RHOGAP3"
                     /note="major polyA site"
ORIGIN      
        1 agcacagggc ttcctgggct ctgcagagca aactggaaag agctgggatc caggcactat
       61 gttctctgaa gaactgtggc tggaaaatga gaaaaagtgt gctgtggttc ggaagtctaa
      121 gcagggcagg aaacgccaag aactgctggc cgtagccttc ggggtgaagg tgggtgtcaa
      181 aggcggcttt ctttggcccc ctctcaaact ctttgcctgt tcacagatct cctccctggt
      241 tcgaagggct gccctcacac acaacgacaa ccacttcaat tatgagaaga cacacaactt
      301 taaggtccac acgttccgag gcccacactg gtgtgaatat tgtgccaatt tcatgtgggg
      361 gctcatcgcc caaggggtcc ggtgctcaga ctgtggattg aacgtacaca aacagtgttc
      421 caagcacgtt cccaatgact gccaacctga tctcaagagg atcaagaaag tgtactgttg
      481 tgacctcaca acacttgtga aggctcacaa cactcagaga cccatggtgg tagacatatg
      541 cattcgggaa attgaagcaa gagaaatctc caatgcagat gagaggctgg aagccgtcca
      601 tgaagtgctg atgctgctgc ctcctgccca ctatgaaacc ctccggtacc taatgatcca
      661 cctcaaaaag gttactatga atgaaaaaga caatttcatg aatgcagaaa atctggggat
      721 cgtgtttggg cccactctga tgaggccccc tgaggacagc accctgacca ccctgcatga
      781 tatgcggtac caaaagctga ttgtgcagat tttaatagaa aacgaagacg ttttattcta
      841 atccatcagg gaaatgagct gaatggcccc agcaccatcc aagttgacac agctaaagga
      901 ataaaaacat ttcttaccac ttgatttgtt ttccaagcaa gtgctagaat ttcctggact
      961 gcagaggatc gctgagtggg gtactgtgtc tcatagacat gcgccacctc cacgtgagaa
     1021 caagggtgaa ggtgagggaa gcccctcacc ttgggtcttt tgctgtgcct cctatgtatg
     1081 tctggtttgc tggaagagtg attaatacat ctttaattta ttaaaaaaca atgtagacct
     1141 ttaaacttca gtcttattgg taataaaagg gaacttaatt catacaggta cttgatacag
     1201 ttatacattt tccacttaca aaaagaagac aattctgtta aatgaaacgt gtatcgtaaa
     1261 atgtattttt attttaccca cgagaatgtt gttattttta gcaaatagaa ctcaatgcag
     1321 tgcattggtt attaccctgt gtaccttgtc cctcattttg ctgtgacacc ctgaaaaagc
     1381 tgaccacaaa tgcagtatta tcttgacata cctctgtcct cctcagtgct ttttaatgaa
     1441 atttcacctg ccccttgtgt tcctgctttg gatagttttt gctcttaagc attggcattc
     1501 ttctattgta tggtatgtat ttatagcaat tgtaggatgg tctgaaatgt ccacacactt
     1561 tctatgcata caaattttgt gaattcccaa agtatgcttt ggaattggtc atagtctttt
     1621 cgtatcatga gtgtgtatcc cagaagatac agaatgcggt cattttacct gaaattattt
     1681 gagaacactg gatgtatctg gtctgtgtaa aaagttagca cttaccctct tttcttcctg
     1741 gccaagccct ctgaattcat ctgttaatat caaattgtat ttttgccaca tttctgtatt
     1801 ggtgaaaagc gaatctgcag aataactgtc aacgtttttc attcttgttt tacaaaccta
     1861 ttttttaaag tgagaataag gttggttttt accaaatatt tgttcctcct tgacaaaagt
     1921 agctctgtgt ggataatatg attttattac tacagttcca gtcacttggt tatatttatc
     1981 ttagcatgag cctttcacac caagatttct atattttgta acataggtga aatatttaaa
     2041 acatcaaaaa ctgtaaatct agatttttaa aaaatccaac tgcaatgtct tttcctttga
     2101 ttgagatgat ttgtgtaaac tcaccagtct tgctttggag tgagcagaag agaatgacta
     2161 ttttacgtgg agcatcattg tgtgactgtt gacctggaca gtcccaaggg ctatgcagat
     2221 ggactccatt ggcacaggga ggtttgacct cttccctgct attatccctc ctcccaactg
     2281 tacagatttg tttgttgtag tttatgtact tcttgatact gtcaaaaaat tatctggaaa
     2341 tggttttatt ttgtgaagtg aacaatactt tgtaatttat gatagtgtaa atggaaggca
     2401 aagcatcaca tgtattaaat tcttctgtct atta
//
