LOCUS       NM_014169                980 bp    mRNA    linear   PRI 21-SEP-2024
DEFINITION  Homo sapiens charged multivesicular body protein 4A (CHMP4A), mRNA.
ACCESSION   NM_014169
VERSION     NM_014169.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 980)
  AUTHORS   Wenzel,D.M., Mackay,D.R., Skalicky,J.J., Paine,E.L., Miller,M.S.,
            Ullman,K.S. and Sundquist,W.I.
  TITLE     Comprehensive analysis of the human ESCRT-III-MIT domain
            interactome reveals new cofactors for cytokinetic abscission
  JOURNAL   Elife 11, e77779 (2022)
   PUBMED   36107470
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 980)
  AUTHORS   Tran,P.T., Chiramel,A.I., Johansson,M. and Melik,W.
  TITLE     Roles of ESCRT Proteins ALIX and CHMP4A and Their Interplay with
            Interferon-Stimulated Gene 15 during Tick-Borne Flavivirus
            Infection
  JOURNAL   J Virol 96 (3), e0162421 (2022)
   PUBMED   34851141
  REMARK    GeneRIF: Roles of ESCRT Proteins ALIX and CHMP4A and Their
            Interplay with Interferon-Stimulated Gene 15 during Tick-Borne
            Flavivirus Infection.
REFERENCE   3  (bases 1 to 980)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 980)
  AUTHORS   Fragoza,R., Das,J., Wierbowski,S.D., Liang,J., Tran,T.N., Liang,S.,
            Beltran,J.F., Rivera-Erick,C.A., Ye,K., Wang,T.Y., Yao,L., Mort,M.,
            Stenson,P.D., Cooper,D.N., Wei,X., Keinan,A., Schimenti,J.C.,
            Clark,A.G. and Yu,H.
  TITLE     Extensive disruption of protein interactions by genetic variants
            across the allele frequency spectrum in human populations
  JOURNAL   Nat Commun 10 (1), 4141 (2019)
   PUBMED   31515488
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 980)
  AUTHORS   Olmos,Y., Hodgson,L., Mantell,J., Verkade,P. and Carlton,J.G.
  TITLE     ESCRT-III controls nuclear envelope reformation
  JOURNAL   Nature 522 (7555), 236-239 (2015)
   PUBMED   26040713
REFERENCE   6  (bases 1 to 980)
  AUTHORS   Martin-Serrano,J., Yarovoy,A., Perez-Caballero,D. and Bieniasz,P.D.
  TITLE     Divergent retroviral late-budding domains recruit vacuolar protein
            sorting factors by using alternative adaptor proteins
  JOURNAL   Proc Natl Acad Sci U S A 100 (21), 12414-12419 (2003)
   PUBMED   14519844
  REMARK    Erratum:[Proc Natl Acad Sci U S A. 2003 Dec 9;100(25):152845.
            Yaravoy, A [corrected to Yarovoy, A]]
REFERENCE   7  (bases 1 to 980)
  AUTHORS   Katoh,K., Shibata,H., Suzuki,H., Nara,A., Ishidoh,K., Kominami,E.,
            Yoshimori,T. and Maki,M.
  TITLE     The ALG-2-interacting protein Alix associates with CHMP4b, a human
            homologue of yeast Snf7 that is involved in multivesicular body
            sorting
  JOURNAL   J Biol Chem 278 (40), 39104-39113 (2003)
   PUBMED   12860994
REFERENCE   8  (bases 1 to 980)
  AUTHORS   von Schwedler,U.K., Stuchell,M., Muller,B., Ward,D.M., Chung,H.Y.,
            Morita,E., Wang,H.E., Davis,T., He,G.P., Cimbora,D.M., Scott,A.,
            Krausslich,H.G., Kaplan,J., Morham,S.G. and Sundquist,W.I.
  TITLE     The protein network of HIV budding
  JOURNAL   Cell 114 (6), 701-713 (2003)
   PUBMED   14505570
REFERENCE   9  (bases 1 to 980)
  AUTHORS   Strack,B., Calistri,A., Craig,S., Popova,E. and Gottlinger,H.G.
  TITLE     AIP1/ALIX is a binding partner for HIV-1 p6 and EIAV p9 functioning
            in virus budding
  JOURNAL   Cell 114 (6), 689-699 (2003)
   PUBMED   14505569
REFERENCE   10 (bases 1 to 980)
  AUTHORS   Lippincott-Schwartz,J., Roberts,T.H. and Hirschberg,K.
  TITLE     Secretory protein trafficking and organelle dynamics in living
            cells
  JOURNAL   Annu Rev Cell Dev Biol 16, 557-589 (2000)
   PUBMED   11031247
  REMARK    Review article
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BM041056.1, BC113533.1 and CB241867.1.
            
            On Nov 30, 2019 this sequence version replaced NM_014169.4.
            
            Summary: CHMP4A belongs to the chromatin-modifying protein/charged
            multivesicular body protein (CHMP) family. These proteins are
            components of ESCRT-III (endosomal sorting complex required for
            transport III), a complex involved in degradation of surface
            receptor proteins and formation of endocytic multivesicular bodies
            (MVBs). Some CHMPs have both nuclear and cytoplasmic/vesicular
            distributions, and one such CHMP, CHMP1A (MIM 164010), is required
            for both MVB formation and regulation of cell cycle progression
            (Tsang et al., 2006 [PubMed 16730941]).[supplied by OMIM, Mar
            2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF212243.1, AF161483.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000347519.12/ ENSP00000324205.11
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-34                BM041056.1         493-526
            35-920              BC113533.1         377-1262
            921-980             CB241867.1         51-110              c
FEATURES             Location/Qualifiers
     source          1..980
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="14"
                     /map="14q12"
     gene            1..980
                     /gene="CHMP4A"
                     /gene_synonym="C14orf123; CHMP4; CHMP4B; hSnf7-1; HSPC134;
                     SHAX2; SNF7; SNF7-1; VPS32-1; VPS32A"
                     /note="charged multivesicular body protein 4A"
                     /db_xref="GeneID:29082"
                     /db_xref="HGNC:HGNC:20274"
                     /db_xref="MIM:610051"
     exon            1..80
                     /gene="CHMP4A"
                     /gene_synonym="C14orf123; CHMP4; CHMP4B; hSnf7-1; HSPC134;
                     SHAX2; SNF7; SNF7-1; VPS32-1; VPS32A"
                     /inference="alignment:Splign:2.1.0"
     CDS             50..718
                     /gene="CHMP4A"
                     /gene_synonym="C14orf123; CHMP4; CHMP4B; hSnf7-1; HSPC134;
                     SHAX2; SNF7; SNF7-1; VPS32-1; VPS32A"
                     /note="Snf7 homologue associated with Alix 2; SNF7 homolog
                     associated with Alix-2; vacuolar protein
                     sorting-associated protein 32-1; chromatin modifying
                     protein 4A"
                     /codon_start=1
                     /product="charged multivesicular body protein 4a"
                     /protein_id="NP_054888.3"
                     /db_xref="CCDS:CCDS9619.2"
                     /db_xref="GeneID:29082"
                     /db_xref="HGNC:HGNC:20274"
                     /db_xref="MIM:610051"
                     /translation="MSGLGRLFGKGKKEKGPTPEEAIQKLKETEKILIKKQEFLEQKI
                     QQELQTAKKYGTKNKRAALQALRRKKRFEQQLAQTDGTLSTLEFQREAIENATTNAEV
                     LRTMELAAQSMKKAYQDMDIDKVDELMTDITEQQEVAQQISDAISRPMGFGDDVDEDE
                     LLEELEELEQEELAQELLNVGDKEEEPSVKLPSVPSTHLPAGPAPKVDEDEEALKQLA
                     EWVS"
     exon            81..230
                     /gene="CHMP4A"
                     /gene_synonym="C14orf123; CHMP4; CHMP4B; hSnf7-1; HSPC134;
                     SHAX2; SNF7; SNF7-1; VPS32-1; VPS32A"
                     /inference="alignment:Splign:2.1.0"
     exon            231..408
                     /gene="CHMP4A"
                     /gene_synonym="C14orf123; CHMP4; CHMP4B; hSnf7-1; HSPC134;
                     SHAX2; SNF7; SNF7-1; VPS32-1; VPS32A"
                     /inference="alignment:Splign:2.1.0"
     exon            409..523
                     /gene="CHMP4A"
                     /gene_synonym="C14orf123; CHMP4; CHMP4B; hSnf7-1; HSPC134;
                     SHAX2; SNF7; SNF7-1; VPS32-1; VPS32A"
                     /inference="alignment:Splign:2.1.0"
     exon            524..659
                     /gene="CHMP4A"
                     /gene_synonym="C14orf123; CHMP4; CHMP4B; hSnf7-1; HSPC134;
                     SHAX2; SNF7; SNF7-1; VPS32-1; VPS32A"
                     /inference="alignment:Splign:2.1.0"
     exon            660..980
                     /gene="CHMP4A"
                     /gene_synonym="C14orf123; CHMP4; CHMP4B; hSnf7-1; HSPC134;
                     SHAX2; SNF7; SNF7-1; VPS32-1; VPS32A"
                     /inference="alignment:Splign:2.1.0"
     regulatory      960..965
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CHMP4A"
                     /gene_synonym="C14orf123; CHMP4; CHMP4B; hSnf7-1; HSPC134;
                     SHAX2; SNF7; SNF7-1; VPS32-1; VPS32A"
                     /note="hexamer: AATAAA"
     polyA_site      980
                     /gene="CHMP4A"
                     /gene_synonym="C14orf123; CHMP4; CHMP4B; hSnf7-1; HSPC134;
                     SHAX2; SNF7; SNF7-1; VPS32-1; VPS32A"
                     /note="major polyA site"
ORIGIN      
        1 ggaagtctgg aggacgctga ggggcggagg cgggagaggc gagctcgcga tgagtggtct
       61 cggcaggctc ttcgggaagg ggaagaagga gaaagggcca acccctgaag aagcaataca
      121 gaaactgaag gagacagaga agatactgat caagaaacag gaatttttgg agcagaagat
      181 tcaacaggag ctacaaacag ccaagaagta tgggaccaag aataagagag ctgccctaca
      241 ggctttgcgg aggaagaaaa gattcgaaca gcagctggca caaactgacg ggacattatc
      301 caccctggag tttcagcgtg aggccattga gaatgccact accaatgcag aagtccttcg
      361 taccatggag cttgctgccc aaagcatgaa gaaggcctac caggacatgg acattgacaa
      421 ggtagatgaa ctgatgactg acatcacgga acaacaggag gtggcccagc agatctcaga
      481 tgccatttct cggcctatgg gctttggaga tgatgtggat gaggatgaac tgctggagga
      541 gctagaggag ctggagcagg aggaattggc ccaggagttg ttaaatgtgg gcgacaagga
      601 agaagaaccc tcagtcaaat tgcctagtgt accttctact catctgccgg cagggccagc
      661 tcccaaagtg gatgaagatg aagaagcact aaagcagttg gctgagtggg tatcctgata
      721 aatctgggct tgtcttccta atgctacctt tgttggtcct ttcttcctta agtgccaagt
      781 gctgagctaa aggaggataa ctttttgggg aagtcatgct gagggtggta gtgtgaccct
      841 gcctgaaaaa agggtctctt accctcccag ccctggctca actctgaaga aggatcttgc
      901 tacagaagga gcccttgggc tcccttctct ttgatagcag ttataatgcc cttgttccca
      961 ataaaactgg gcagatggaa
//
