LOCUS       NM_014453                894 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens charged multivesicular body protein 2A (CHMP2A),
            transcript variant 2, mRNA.
ACCESSION   NM_014453
VERSION     NM_014453.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Wenzel,D.M., Mackay,D.R., Skalicky,J.J., Paine,E.L., Miller,M.S.,
            Ullman,K.S. and Sundquist,W.I.
  TITLE     Comprehensive analysis of the human ESCRT-III-MIT domain
            interactome reveals new cofactors for cytokinetic abscission
  JOURNAL   Elife 11, e77779 (2022)
   PUBMED   36107470
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 894)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   3  (bases 1 to 894)
  AUTHORS   Wang,H., Wang,Y., Wang,X., Huang,H., Bao,J., Zhong,W. and Li,A.
  TITLE     PTEN alleviates maladaptive repair of renal tubular epithelial
            cells by restoring CHMP2A-mediated phagosome closure
  JOURNAL   Cell Death Dis 12 (12), 1087 (2021)
   PUBMED   34789720
  REMARK    GeneRIF: PTEN alleviates maladaptive repair of renal tubular
            epithelial cells by restoring CHMP2A-mediated phagosome closure.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 894)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   5  (bases 1 to 894)
  AUTHORS   Alqabandi,M., de Franceschi,N., Maity,S., Miguet,N., Bally,M.,
            Roos,W.H., Weissenhorn,W., Bassereau,P. and Mangenot,S.
  TITLE     The ESCRT-III isoforms CHMP2A and CHMP2B display different effects
            on membranes upon polymerization
  JOURNAL   BMC Biol 19 (1), 66 (2021)
   PUBMED   33832485
  REMARK    GeneRIF: The ESCRT-III isoforms CHMP2A and CHMP2B display different
            effects on membranes upon polymerization.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 894)
  AUTHORS   von Schwedler,U.K., Stuchell,M., Muller,B., Ward,D.M., Chung,H.Y.,
            Morita,E., Wang,H.E., Davis,T., He,G.P., Cimbora,D.M., Scott,A.,
            Krausslich,H.G., Kaplan,J., Morham,S.G. and Sundquist,W.I.
  TITLE     The protein network of HIV budding
  JOURNAL   Cell 114 (6), 701-713 (2003)
   PUBMED   14505570
REFERENCE   7  (bases 1 to 894)
  AUTHORS   Strack,B., Calistri,A., Craig,S., Popova,E. and Gottlinger,H.G.
  TITLE     AIP1/ALIX is a binding partner for HIV-1 p6 and EIAV p9 functioning
            in virus budding
  JOURNAL   Cell 114 (6), 689-699 (2003)
   PUBMED   14505569
REFERENCE   8  (bases 1 to 894)
  AUTHORS   Gevaert,K., Goethals,M., Martens,L., Van Damme,J., Staes,A.,
            Thomas,G.R. and Vandekerckhove,J.
  TITLE     Exploring proteomes and analyzing protein processing by mass
            spectrometric identification of sorted N-terminal peptides
  JOURNAL   Nat Biotechnol 21 (5), 566-569 (2003)
   PUBMED   12665801
REFERENCE   9  (bases 1 to 894)
  AUTHORS   Howard,T.L., Stauffer,D.R., Degnin,C.R. and Hollenberg,S.M.
  TITLE     CHMP1 functions as a member of a newly defined family of vesicle
            trafficking proteins
  JOURNAL   J Cell Sci 114 (Pt 13), 2395-2404 (2001)
   PUBMED   11559748
REFERENCE   10 (bases 1 to 894)
  AUTHORS   Lippincott-Schwartz,J., Roberts,T.H. and Hirschberg,K.
  TITLE     Secretory protein trafficking and organelle dynamics in living
            cells
  JOURNAL   Annu Rev Cell Dev Biol 16, 557-589 (2000)
   PUBMED   11031247
  REMARK    Review article
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BP315111.1 and BC002502.2.
            
            On May 17, 2019 this sequence version replaced NM_014453.3.
            
            Summary: CHMP2A belongs to the chromatin-modifying protein/charged
            multivesicular body protein (CHMP) family. These proteins are
            components of ESCRT-III (endosomal sorting complex required for
            transport III), a complex involved in degradation of surface
            receptor proteins and formation of endocytic multivesicular bodies
            (MVBs). Some CHMPs have both nuclear and cytoplasmic/vesicular
            distributions, and one such CHMP, CHMP1A (MIM 164010), is required
            for both MVB formation and regulation of cell cycle progression
            (Tsang et al., 2006 [PubMed 16730941]).[supplied by OMIM, Mar
            2008].
            
            Transcript Variant: This variant (2) differs in the 5' UTR compared
            to variant 1. Variants 1 and 2 encode the same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR7346977.687878.1,
                                           SRR5189652.111156.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN03267753, SAMN03267755
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000312547.7/ ENSP00000310440.1
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-414               BP315111.1         20-433
            415-894             BC002502.2         406-885
FEATURES             Location/Qualifiers
     source          1..894
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19q13.43"
     gene            1..894
                     /gene="CHMP2A"
                     /gene_synonym="BC-2; BC2; CHMP2; VPS2; VPS2A"
                     /note="charged multivesicular body protein 2A"
                     /db_xref="GeneID:27243"
                     /db_xref="HGNC:HGNC:30216"
                     /db_xref="MIM:610893"
     exon            1..118
                     /gene="CHMP2A"
                     /gene_synonym="BC-2; BC2; CHMP2; VPS2; VPS2A"
                     /inference="alignment:Splign:2.1.0"
     exon            119..310
                     /gene="CHMP2A"
                     /gene_synonym="BC-2; BC2; CHMP2; VPS2; VPS2A"
                     /inference="alignment:Splign:2.1.0"
     CDS             143..811
                     /gene="CHMP2A"
                     /gene_synonym="BC-2; BC2; CHMP2; VPS2; VPS2A"
                     /note="putative breast adenocarcinoma marker (32kD); VPS2
                     homolog A; vps2-1; putative breast adenocarcinoma marker
                     BC-2; vacuolar protein sorting-associated protein 2-1;
                     chromatin modifying protein 2A"
                     /codon_start=1
                     /product="charged multivesicular body protein 2a"
                     /protein_id="NP_055268.1"
                     /db_xref="CCDS:CCDS12986.1"
                     /db_xref="GeneID:27243"
                     /db_xref="HGNC:HGNC:30216"
                     /db_xref="MIM:610893"
                     /translation="MDLLFGRRKTPEELLRQNQRALNRAMRELDRERQKLETQEKKII
                     ADIKKMAKQGQMDAVRIMAKDLVRTRRYVRKFVLMRANIQAVSLKIQTLKSNNSMAQA
                     MKGVTKAMGTMNRQLKLPQIQKIMMEFERQAEIMDMKEEMMNDAIDDAMGDEEDEEES
                     DAVVSQVLDELGLSLTDELSNLPSTGGSLSVAAGGKKAEAAASALADADADLEERLKN
                     LRRD"
     exon            311..490
                     /gene="CHMP2A"
                     /gene_synonym="BC-2; BC2; CHMP2; VPS2; VPS2A"
                     /inference="alignment:Splign:2.1.0"
     exon            491..621
                     /gene="CHMP2A"
                     /gene_synonym="BC-2; BC2; CHMP2; VPS2; VPS2A"
                     /inference="alignment:Splign:2.1.0"
     exon            622..683
                     /gene="CHMP2A"
                     /gene_synonym="BC-2; BC2; CHMP2; VPS2; VPS2A"
                     /inference="alignment:Splign:2.1.0"
     exon            684..894
                     /gene="CHMP2A"
                     /gene_synonym="BC-2; BC2; CHMP2; VPS2; VPS2A"
                     /inference="alignment:Splign:2.1.0"
     regulatory      874..879
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CHMP2A"
                     /gene_synonym="BC-2; BC2; CHMP2; VPS2; VPS2A"
                     /note="hexamer: AATAAA"
     polyA_site      894
                     /gene="CHMP2A"
                     /gene_synonym="BC-2; BC2; CHMP2; VPS2; VPS2A"
                     /note="major polyA site"
ORIGIN      
        1 aatccggaaa cggcggcggc ggcgacagga ccgaggggcc ttagttggtg ggcaagtcgg
       61 ggatcccaga aagagaagcg tgacccggaa gcggaaacgg gtgtccgtcc cagctccggc
      121 ctgccagtga gcttctacca tcatggacct attgttcggg cgccggaaga cgccagagga
      181 gctactgcgg cagaaccaga gggccctgaa ccgtgccatg cgggagctgg accgcgagcg
      241 acagaaacta gagacccagg agaagaaaat cattgcagac attaagaaga tggccaagca
      301 aggccagatg gatgctgttc gcatcatggc aaaagacttg gtgcgcaccc ggcgctatgt
      361 gcgcaagttt gtattgatgc gggccaacat ccaggctgtg tccctcaaga tccagacact
      421 caagtccaac aactcgatgg cacaagccat gaagggtgtc accaaggcca tgggcaccat
      481 gaacagacag ctgaagttgc cccagatcca gaagatcatg atggagtttg agcggcaggc
      541 agagatcatg gatatgaagg aggagatgat gaatgatgcc attgatgatg ccatgggtga
      601 tgaggaagat gaagaggaga gtgatgctgt ggtgtcccag gttctggatg agctgggact
      661 tagcctaaca gatgagctgt cgaacctccc ctcaactggg ggctcgctta gtgtggctgc
      721 tggtgggaaa aaagcagagg ccgcagcctc agccctagct gatgctgatg cagacctgga
      781 ggaacggctt aagaacctgc ggagggactg agtgcccctg ccactccgag ataaccagtg
      841 gatgcccagg atcttttacc acaacccctc tgtaataaaa gagatttgac acta
//
