LOCUS       NM_001277               2714 bp    mRNA    linear   PRI 02-APR-2024
DEFINITION  Homo sapiens choline kinase alpha (CHKA), transcript variant 1,
            mRNA.
ACCESSION   NM_001277
VERSION     NM_001277.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Ozawa,A., Iwasaki,M., Yokoyama,K., Tsuchiya,J., Kawano,R.,
            Nishihara,H. and Tateishi,U.
  TITLE     Correlation between choline kinase alpha expression and 11C-choline
            accumulation in breast cancer using positron emission
            tomography/computed tomography: a retrospective study
  JOURNAL   Sci Rep 13 (1), 17620 (2023)
   PUBMED   37848481
  REMARK    GeneRIF: Correlation between choline kinase alpha expression and
            [11]C-choline accumulation in breast cancer using positron emission
            tomography/computed tomography: a retrospective study.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2714)
  AUTHORS   Klockner,C., Fernandez-Murray,J.P., Tavasoli,M., Sticht,H.,
            Stoltenburg-Didinger,G., Scholle,L.M., Bakhtiari,S., Kruer,M.C.,
            Darvish,H., Firouzabadi,S.G., Pagnozzi,A., Shukla,A., Girisha,K.M.,
            Narayanan,D.L., Kaur,P., Maroofian,R., Zaki,M.S., Noureldeen,M.M.,
            Merkenschlager,A., Gburek-Augustat,J., Cali,E., Banu,S., Nahar,K.,
            Efthymiou,S., Houlden,H., Jamra,R.A., Williams,J., McMaster,C.R.
            and Platzer,K.
  TITLE     Bi-allelic variants in CHKA cause a neurodevelopmental disorder
            with epilepsy and microcephaly
  JOURNAL   Brain 145 (6), 1916-1923 (2022)
   PUBMED   35202461
  REMARK    GeneRIF: Bi-allelic variants in CHKA cause a neurodevelopmental
            disorder with epilepsy and microcephaly.
REFERENCE   3  (bases 1 to 2714)
  AUTHORS   Liu,R., Lee,J.H., Li,J., Yu,R., Tan,L., Xia,Y., Zheng,Y.,
            Bian,X.L., Lorenzi,P.L., Chen,Q. and Lu,Z.
  TITLE     Choline kinase alpha 2 acts as a protein kinase to promote
            lipolysis of lipid droplets
  JOURNAL   Mol Cell 81 (13), 2722-2735 (2021)
   PUBMED   34077757
  REMARK    GeneRIF: Choline kinase alpha 2 acts as a protein kinase to promote
            lipolysis of lipid droplets.
REFERENCE   4  (bases 1 to 2714)
  AUTHORS   Mohamed Sa'dom,S.A.F., Raikundalia,S., Shamsuddin,S., See Too,W.C.
            and Few,L.L.
  TITLE     DNA Methylation of Human Choline Kinase Alpha Promoter-Associated
            CpG Islands in MCF-7 Cells
  JOURNAL   Genes (Basel) 12 (6), 853 (2021)
   PUBMED   34205960
  REMARK    GeneRIF: DNA Methylation of Human Choline Kinase Alpha
            Promoter-Associated CpG Islands in MCF-7 Cells.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2714)
  AUTHORS   Yue,F., Zou,Y., Sun,S., Wang,Z., Huang,L. and Ma,H.
  TITLE     [Knockdown of choline kinase alpha (CHKA) inhibits the
            proliferation, invasion and migration of human U87MG glioma cells]
  JOURNAL   Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 36 (8), 724-728 (2020)
   PUBMED   32958130
  REMARK    GeneRIF: [Knockdown of choline kinase alpha (CHKA) inhibits the
            proliferation, invasion and migration of human U87MG glioma cells].
REFERENCE   6  (bases 1 to 2714)
  AUTHORS   Price,D.J., Rivnay,B., Fu,Y., Jiang,S., Avraham,S. and Avraham,H.
  TITLE     Direct association of Csk homologous kinase (CHK) with the
            diphosphorylated site Tyr568/570 of the activated c-KIT in
            megakaryocytes
  JOURNAL   J Biol Chem 272 (9), 5915-5920 (1997)
   PUBMED   9038210
REFERENCE   7  (bases 1 to 2714)
  AUTHORS   Kaplan,O. and Cohen,J.S.
  TITLE     Lymphocyte activation-31P magnetic resonance studies of energy
            metabolism and phospholipid pathways
  JOURNAL   Immunomethods 4 (2), 139-147 (1994)
   PUBMED   8069533
REFERENCE   8  (bases 1 to 2714)
  AUTHORS   Hosaka,K., Tanaka,S., Nikawa,J. and Yamashita,S.
  TITLE     Cloning of a human choline kinase cDNA by complementation of the
            yeast cki mutation
  JOURNAL   FEBS Lett 304 (2-3), 229-232 (1992)
   PUBMED   1618328
REFERENCE   9  (bases 1 to 2714)
  AUTHORS   Spanner,S. and Ansell,G.B.
  TITLE     Choline kinase and ethanolamine kinase activity in the cytosol of
            nerve endings from rat forebrain
  JOURNAL   Biochem J 178 (3), 753-760 (1979)
   PUBMED   36885
REFERENCE   10 (bases 1 to 2714)
  AUTHORS   Spanner,S. and Ansell,G.B.
  TITLE     Choline and ethanolamine kinase activity in the cytoplasm of nerve
            endings from rat forebrain
  JOURNAL   Adv Exp Med Biol 101, 237-245 (1978)
   PUBMED   208357
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BG723115.1, BG703955.1,
            BC036471.2, D10704.1, BI195927.1 and BU617910.1.
            
            On Nov 22, 2018 this sequence version replaced NM_001277.2.
            
            Summary: The major pathway for the biosynthesis of
            phosphatidylcholine occurs via the CDP-choline pathway. The protein
            encoded by this gene is the initial enzyme in the sequence and may
            play a regulatory role. The encoded protein also catalyzes the
            phosphorylation of ethanolamine. Two transcript variants encoding
            different isoforms have been found for this gene. [provided by
            RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: D10704.1, SRR1803611.135921.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2161674 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000265689.9/ ENSP00000265689.4
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-52                BG723115.1         65-116
            53-119              BG703955.1         62-128
            120-670             BC036471.2         1-551
            671-1642            D10704.1           484-1455
            1643-2149           BI195927.1         207-713
            2150-2174           BI195927.1         715-739
            2175-2186           D10704.1           1992-2003
            2187-2201           BI195927.1         749-763
            2202-2229           D10704.1           2018-2045
            2230-2714           BU617910.1         17-501              c
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11q13.2"
     gene            1..2714
                     /gene="CHKA"
                     /gene_synonym="CHK; CK; CKI; EK; NEDMIMS"
                     /note="choline kinase alpha"
                     /db_xref="GeneID:1119"
                     /db_xref="HGNC:HGNC:1937"
                     /db_xref="MIM:118491"
     exon            1..561
                     /gene="CHKA"
                     /gene_synonym="CHK; CK; CKI; EK; NEDMIMS"
                     /inference="alignment:Splign:2.1.0"
     CDS             212..1585
                     /gene="CHKA"
                     /gene_synonym="CHK; CK; CKI; EK; NEDMIMS"
                     /EC_number="2.7.1.32"
                     /EC_number="2.7.1.82"
                     /note="isoform a is encoded by transcript variant 1;
                     CHETK-alpha; ethanolamine kinase"
                     /codon_start=1
                     /product="choline kinase alpha isoform a"
                     /protein_id="NP_001268.2"
                     /db_xref="CCDS:CCDS8178.1"
                     /db_xref="GeneID:1119"
                     /db_xref="HGNC:HGNC:1937"
                     /db_xref="MIM:118491"
                     /translation="MKTKFCTGGEAEPSPLGLLLSCGSGSAAPAPGVGQQRDAASDLE
                     SKQLGGQQPPLALPPPPPLPLPLPLPQPPPPQPPADEQPEPRTRRRAYLWCKEFLPGA
                     WRGLREDEFHISVIRGGLSNMLFQCSLPDTTATLGDEPRKVLLRLYGAILQMRSCNKE
                     GSEQAQKENEFQGAEAMVLESVMFAILAERSLGPKLYGIFPQGRLEQFIPSRRLDTEE
                     LSLPDISAEIAEKMATFHGMKMPFNKEPKWLFGTMEKYLKEVLRIKFTEESRIKKLHK
                     LLSYNLPLELENLRSLLESTPSPVVFCHNDCQEGNILLLEGRENSEKQKLMLIDFEYS
                     SYNYRGFDIGNHFCEWMYDYSYEKYPFFRANIRKYPTKKQQLHFISSYLPAFQNDFEN
                     LSTEEKSIIKEEMLLEVNRFALASHFLWGLWSIVQAKISSIEFGYMDYAQARFDAYFH
                     QKRKLGV"
     exon            562..673
                     /gene="CHKA"
                     /gene_synonym="CHK; CK; CKI; EK; NEDMIMS"
                     /inference="alignment:Splign:2.1.0"
     exon            674..727
                     /gene="CHKA"
                     /gene_synonym="CHK; CK; CKI; EK; NEDMIMS"
                     /inference="alignment:Splign:2.1.0"
     exon            728..841
                     /gene="CHKA"
                     /gene_synonym="CHK; CK; CKI; EK; NEDMIMS"
                     /inference="alignment:Splign:2.1.0"
     exon            842..975
                     /gene="CHKA"
                     /gene_synonym="CHK; CK; CKI; EK; NEDMIMS"
                     /inference="alignment:Splign:2.1.0"
     exon            976..1080
                     /gene="CHKA"
                     /gene_synonym="CHK; CK; CKI; EK; NEDMIMS"
                     /inference="alignment:Splign:2.1.0"
     exon            1081..1139
                     /gene="CHKA"
                     /gene_synonym="CHK; CK; CKI; EK; NEDMIMS"
                     /inference="alignment:Splign:2.1.0"
     exon            1140..1227
                     /gene="CHKA"
                     /gene_synonym="CHK; CK; CKI; EK; NEDMIMS"
                     /inference="alignment:Splign:2.1.0"
     exon            1228..1336
                     /gene="CHKA"
                     /gene_synonym="CHK; CK; CKI; EK; NEDMIMS"
                     /inference="alignment:Splign:2.1.0"
     exon            1337..1443
                     /gene="CHKA"
                     /gene_synonym="CHK; CK; CKI; EK; NEDMIMS"
                     /inference="alignment:Splign:2.1.0"
     exon            1444..1525
                     /gene="CHKA"
                     /gene_synonym="CHK; CK; CKI; EK; NEDMIMS"
                     /inference="alignment:Splign:2.1.0"
     exon            1526..2714
                     /gene="CHKA"
                     /gene_synonym="CHK; CK; CKI; EK; NEDMIMS"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2694..2699
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CHKA"
                     /gene_synonym="CHK; CK; CKI; EK; NEDMIMS"
                     /note="hexamer: AATAAA"
     polyA_site      2714
                     /gene="CHKA"
                     /gene_synonym="CHK; CK; CKI; EK; NEDMIMS"
                     /note="major polyA site"
ORIGIN      
        1 agaggagcga gtgcagcggc cagcagcaca tccccgctcc acagtcgccg cagtcgccgc
       61 agccgccgcc gccgccccgc gcgcccaacc gccgcggccc cctgccccgc cggcctgcca
      121 gtgagagagc ggcgaggggg cgcccggccg gactctgagc ctagtcctct cgcgctgcgg
      181 ccgcccgcgc ctcctcggcc gcctgtcggg catgaaaacc aaattctgca ccgggggcga
      241 ggcggagccc tcgccgctcg ggctgctgct gagctgcggt agcggcagcg cggccccggc
      301 gcccggcgtg gggcagcagc gcgacgccgc cagcgacctc gagtccaagc agctgggcgg
      361 ccaacagccg ccgctcgcgc tgccccctcc gccgccgctg ccgctgccgc tgccgctgcc
      421 ccagcccccg ccgccgcagc cgcccgcaga cgagcagccg gagccccgga cgcggcgcag
      481 ggcctatctg tggtgcaagg agttcctgcc cggcgcctgg cggggcctcc gcgaggacga
      541 gttccacatc agtgtcatca gaggcggcct tagcaacatg ctgttccagt gctccctacc
      601 tgacaccaca gccacccttg gtgatgagcc tcggaaagtg ctcctgcggc tgtatggagc
      661 gattttgcag atgaggtcct gtaataaaga gggatccgaa caagctcaga aagaaaatga
      721 atttcaaggg gctgaggcca tggttctgga gagcgttatg tttgccattc tcgcagagag
      781 gtcacttggg ccaaaactct atggcatctt tccccaaggc cgactggagc agttcatccc
      841 gagccggcga ttagatactg aagaattaag tttgccagat atttctgcag aaatcgccga
      901 gaaaatggct acatttcatg gtatgaaaat gccattcaat aaggaaccaa aatggctttt
      961 tggcacaatg gaaaagtatc taaaggaagt gctgagaatt aaatttactg aggaatccag
     1021 aattaaaaag ctccacaaat tgctcagtta caatctgccc ttggaactgg aaaacctgag
     1081 atcattgctt gaatctactc catctccagt tgtattttgt cataatgact gtcaagaagg
     1141 taatatcttg ttgctggaag gccgagagaa ttctgaaaaa cagaaactga tgctcattga
     1201 tttcgaatac agcagttaca attacagggg attcgacatt ggaaatcact tctgtgagtg
     1261 gatgtatgat tatagctatg aaaaataccc ttttttcaga gcaaacatcc ggaagtatcc
     1321 caccaagaaa caacagctcc attttatttc cagttacttg cctgcattcc aaaatgactt
     1381 tgaaaacctc agtactgaag aaaaatccat tataaaagaa gaaatgttgc ttgaagttaa
     1441 taggtttgcc cttgcatctc atttcctctg gggactgtgg tccattgtac aagccaagat
     1501 ttcatctatt gaatttgggt acatggacta cgcccaagca aggtttgatg cctatttcca
     1561 ccagaagagg aagcttgggg tgtgactgtg gggaggactc catccacctc atcactggac
     1621 tgcatgggga ggcagcagag cggggtcccc tctgtgcttc gactactgct cctgtggcag
     1681 gaggctttgg gtggctcact actgaacaca tgtgtatgat actaaagacg gtattaaaat
     1741 ggagcgacgt ttatttcatc tcttgtttac gatttcacta ggactcagaa acgagatcgg
     1801 gaagcagaaa tatagtgcaa tagtgcaaca tctctgaatc cttttaatct agagaaggca
     1861 tttcatattt gggggctaag gtttccagtc agatgaggca aacagcaaga gtaagcagtg
     1921 ttacttgcag gtactttggt taatgttgat ttaaattttc atgaatgtgc tggtgaacac
     1981 tgtgaccagg cttttgtaga tggcgatgtg ttatagacgg tgctcactcc caagggacag
     2041 caagtgagca gagatgtact gcaaagtcgc cagtcactgc tgcaaggtgg cctctgcctg
     2101 gggcctccag aagctgctcc tttaccctct tggtcccatg gctgaagctg gagcagcgga
     2161 ttgctctgga gcagccaagg ccgccagcgt gtggagcaga gctctcccct cctgctgggc
     2221 gtgtgtgaca ctgatgagtt tcactgtact gcatgtgact tctcccctgc ccttcctcct
     2281 gatggagtgt gcagacagcc atgcgtggcc acgggggcag tgtgaggacc tccctgtctc
     2341 ccggctcccc tcccagggga gccagctgct tgacctagct ctttgggcct ctcctgccct
     2401 ctgctctgcc tggagtgtcg gatcctgtga gtaggctggg cctcccctgg gcagggttct
     2461 ccaagggccc ggtttcccgg cccttacctt tcctgatgcc cctgacatca tcattcttgt
     2521 gggagacagc agcctgtatg tggtgtgggg cgtggatcga gtgtagctgt gaaatccata
     2581 tatatgaaat gtcctgcggg atacagtctt agctgacttt tttttactct gaactcttat
     2641 ttgaattgtt ttttgtgcat atatttctgc taccacagag attgtactat acaaataaaa
     2701 aaataaaaac ccaa
//
