LOCUS       NM_001114122            4128 bp    mRNA    linear   PRI 23-JUL-2024
DEFINITION  Homo sapiens checkpoint kinase 1 (CHEK1), transcript variant 1,
            mRNA.
ACCESSION   NM_001114122
VERSION     NM_001114122.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4128)
  AUTHORS   Wang,J., Liu,Y., Wu,D., Tian,C., Gao,J., Yang,Q., Hong,X., Gu,F.,
            Zhang,K., Hu,Y., Xu,S., Liu,L. and Zeng,Y.
  TITLE     OTUB1 Targets CHK1 for Deubiquitination and Stabilization to
            Facilitate Lung Cancer Progression and Radioresistance
  JOURNAL   Int J Radiat Oncol Biol Phys 119 (4), 1222-1233 (2024)
   PUBMED   38266782
  REMARK    GeneRIF: OTUB1 Targets CHK1 for Deubiquitination and Stabilization
            to Facilitate Lung Cancer Progression and Radioresistance.
REFERENCE   2  (bases 1 to 4128)
  AUTHORS   Ando,S., Tanaka,K., Matsumoto,M., Oyama,Y., Tomabechi,Y.,
            Yamagata,A., Shirouzu,M., Nakagawa,R., Okimoto,N., Taiji,M.,
            Sato,K. and Ohama,T.
  TITLE     The luciferase-based in vivo protein-protein interaction assay
            revealed that CHK1 promotes PP2A and PME-1 interaction
  JOURNAL   J Biol Chem 300 (5), 107277 (2024)
   PUBMED   38588804
  REMARK    GeneRIF: The luciferase-based in vivo protein-protein interaction
            assay revealed that CHK1 promotes PP2A and PME-1 interaction.
REFERENCE   3  (bases 1 to 4128)
  AUTHORS   Egger,T., Morano,L., Blanchard,M.P., Basbous,J. and Constantinou,A.
  TITLE     Spatial organization and functions of Chk1 activation by TopBP1
            biomolecular condensates
  JOURNAL   Cell Rep 43 (4), 114064 (2024)
   PUBMED   38578830
  REMARK    GeneRIF: Spatial organization and functions of Chk1 activation by
            TopBP1 biomolecular condensates.
REFERENCE   4  (bases 1 to 4128)
  AUTHORS   Colaco,V., Goswami,N., Goel,V.K., Srivastava,S.K., Lalrohlua,P.,
            Senthil Kumar,N., Borah,P., Baruah,R. and Varma,A.K.
  TITLE     In silico and structure-based evaluation of deleterious mutations
            identified in human Chk1, Chk2, and Wee1 protein kinase
  JOURNAL   J Cell Biochem 125 (1), 89-99 (2024)
   PUBMED   38047473
  REMARK    GeneRIF: In silico and structure-based evaluation of deleterious
            mutations identified in human Chk1, Chk2, and Wee1 protein kinase.
REFERENCE   5  (bases 1 to 4128)
  AUTHORS   Jaiswal,R.K., Lei,K.H., Chastain,M., Wang,Y., Shiva,O., Li,S.,
            You,Z., Chi,P. and Chai,W.
  TITLE     CaMKK2 and CHK1 phosphorylate human STN1 in response to replication
            stress to protect stalled forks from aberrant resection
  JOURNAL   Nat Commun 14 (1), 7882 (2023)
   PUBMED   38036565
  REMARK    GeneRIF: CaMKK2 and CHK1 phosphorylate human STN1 in response to
            replication stress to protect stalled forks from aberrant
            resection.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4128)
  AUTHORS   Ouyang,B., Li,W., Pan,H., Meadows,J., Hoffmann,I. and Dai,W.
  TITLE     The physical association and phosphorylation of Cdc25C protein
            phosphatase by Prk
  JOURNAL   Oncogene 18 (44), 6029-6036 (1999)
   PUBMED   10557092
REFERENCE   7  (bases 1 to 4128)
  AUTHORS   Giaccia,A.J. and Kastan,M.B.
  TITLE     The complexity of p53 modulation: emerging patterns from divergent
            signals
  JOURNAL   Genes Dev 12 (19), 2973-2983 (1998)
   PUBMED   9765199
  REMARK    Review article
REFERENCE   8  (bases 1 to 4128)
  AUTHORS   Flaggs,G., Plug,A.W., Dunks,K.M., Mundt,K.E., Ford,J.C.,
            Quiggle,M.R., Taylor,E.M., Westphal,C.H., Ashley,T., Hoekstra,M.F.
            and Carr,A.M.
  TITLE     Atm-dependent interactions of a mammalian chk1 homolog with meiotic
            chromosomes
  JOURNAL   Curr Biol 7 (12), 977-986 (1997)
   PUBMED   9382850
REFERENCE   9  (bases 1 to 4128)
  AUTHORS   Peng,C.Y., Graves,P.R., Thoma,R.S., Wu,Z., Shaw,A.S. and
            Piwnica-Worms,H.
  TITLE     Mitotic and G2 checkpoint control: regulation of 14-3-3 protein
            binding by phosphorylation of Cdc25C on serine-216
  JOURNAL   Science 277 (5331), 1501-1505 (1997)
   PUBMED   9278512
REFERENCE   10 (bases 1 to 4128)
  AUTHORS   Sanchez,Y., Wong,C., Thoma,R.S., Richman,R., Wu,Z.,
            Piwnica-Worms,H. and Elledge,S.J.
  TITLE     Conservation of the Chk1 checkpoint pathway in mammals: linkage of
            DNA damage to Cdk regulation through Cdc25
  JOURNAL   Science 277 (5331), 1497-1501 (1997)
   PUBMED   9278511
  REMARK    GeneRIF: Biochemical studies suggest the functional interactions of
            this protein with cell cycle regulators. Cdc25A, Cdc25B, and Cdc25C
            can be phosphoryalted by this protein.
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AP001132.4.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Sep 18, 2020 this sequence version replaced NM_001114122.2.
            
            Summary: The protein encoded by this gene belongs to the Ser/Thr
            protein kinase family. It is required for checkpoint mediated cell
            cycle arrest in response to DNA damage or the presence of
            unreplicated DNA. This protein acts to integrate signals from ATM
            and ATR, two cell cycle proteins involved in DNA damage responses,
            that also associate with chromatin in meiotic prophase I.
            Phosphorylation of CDC25A protein phosphatase by this protein is
            required for cells to delay cell cycle progression in response to
            double-strand DNA breaks. Several alternatively spliced transcript
            variants have been found for this gene. [provided by RefSeq, Oct
            2011].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14243140.8673842.1,
                                           SRR1660807.203825.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000438015.7/ ENSP00000388648.1
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-850               AP001132.4         18495-19344
            851-935             AP001132.4         20081-20165
            936-1159            AP001132.4         20939-21162
            1160-1224           AP001132.4         22564-22628
            1225-1294           AP001132.4         22723-22792
            1295-1483           AP001132.4         26495-26683
            1484-1588           AP001132.4         28761-28865
            1589-1684           AP001132.4         30781-30876
            1685-1793           AP001132.4         37124-37232
            1794-1971           AP001132.4         37423-37600
            1972-2103           AP001132.4         37844-37975
            2104-2205           AP001132.4         47078-47179
            2206-4128           AP001132.4         48557-50479
FEATURES             Location/Qualifiers
     source          1..4128
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11q24.2"
     gene            1..4128
                     /gene="CHEK1"
                     /gene_synonym="CHK1; OZEMA21"
                     /note="checkpoint kinase 1"
                     /db_xref="GeneID:1111"
                     /db_xref="HGNC:HGNC:1925"
                     /db_xref="MIM:603078"
     exon            1..850
                     /gene="CHEK1"
                     /gene_synonym="CHK1; OZEMA21"
                     /inference="alignment:Splign:2.1.0"
     exon            851..935
                     /gene="CHEK1"
                     /gene_synonym="CHK1; OZEMA21"
                     /inference="alignment:Splign:2.1.0"
     CDS             871..2301
                     /gene="CHEK1"
                     /gene_synonym="CHK1; OZEMA21"
                     /EC_number="2.7.11.1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     Checkpoint, S. pombe, homolog of, 1;
                     serine/threonine-protein kinase Chk1; CHK1 checkpoint
                     homolog; cell cycle checkpoint kinase; Chk1-S"
                     /codon_start=1
                     /product="serine/threonine-protein kinase Chk1 isoform 1"
                     /protein_id="NP_001107594.1"
                     /db_xref="CCDS:CCDS8459.1"
                     /db_xref="GeneID:1111"
                     /db_xref="HGNC:HGNC:1925"
                     /db_xref="MIM:603078"
                     /translation="MAVPFVEDWDLVQTLGEGAYGEVQLAVNRVTEEAVAVKIVDMKR
                     AVDCPENIKKEICINKMLNHENVVKFYGHRREGNIQYLFLEYCSGGELFDRIEPDIGM
                     PEPDAQRFFHQLMAGVVYLHGIGITHRDIKPENLLLDERDNLKISDFGLATVFRYNNR
                     ERLLNKMCGTLPYVAPELLKRREFHAEPVDVWSCGIVLTAMLAGELPWDQPSDSCQEY
                     SDWKEKKTYLNPWKKIDSAPLALLHKILVENPSARITIPDIKKDRWYNKPLKKGAKRP
                     RVTSGGVSESPSGFSKHIQSNLDFSPVNSASSEENVKYSSSQPEPRTGLSLWDTSPSY
                     IDKLVQGISFSQPTCPDHMLLNSQLLGTPGSSQNPWQRLVKRMTRFFTKLDADKSYQC
                     LKETCEKLGYQWKKSCMNQVTISTTDRRNNKLIFKVNLLEMDDKILVDFRLSKGDGLE
                     FKRHFLKIKGKLIDIVSSQKIWLPAT"
     exon            936..1159
                     /gene="CHEK1"
                     /gene_synonym="CHK1; OZEMA21"
                     /inference="alignment:Splign:2.1.0"
     exon            1160..1224
                     /gene="CHEK1"
                     /gene_synonym="CHK1; OZEMA21"
                     /inference="alignment:Splign:2.1.0"
     exon            1225..1294
                     /gene="CHEK1"
                     /gene_synonym="CHK1; OZEMA21"
                     /inference="alignment:Splign:2.1.0"
     exon            1295..1483
                     /gene="CHEK1"
                     /gene_synonym="CHK1; OZEMA21"
                     /inference="alignment:Splign:2.1.0"
     exon            1484..1588
                     /gene="CHEK1"
                     /gene_synonym="CHK1; OZEMA21"
                     /inference="alignment:Splign:2.1.0"
     exon            1589..1684
                     /gene="CHEK1"
                     /gene_synonym="CHK1; OZEMA21"
                     /inference="alignment:Splign:2.1.0"
     exon            1685..1793
                     /gene="CHEK1"
                     /gene_synonym="CHK1; OZEMA21"
                     /inference="alignment:Splign:2.1.0"
     exon            1794..1971
                     /gene="CHEK1"
                     /gene_synonym="CHK1; OZEMA21"
                     /inference="alignment:Splign:2.1.0"
     exon            1972..2103
                     /gene="CHEK1"
                     /gene_synonym="CHK1; OZEMA21"
                     /inference="alignment:Splign:2.1.0"
     exon            2104..2205
                     /gene="CHEK1"
                     /gene_synonym="CHK1; OZEMA21"
                     /inference="alignment:Splign:2.1.0"
     exon            2206..4128
                     /gene="CHEK1"
                     /gene_synonym="CHK1; OZEMA21"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2705..2710
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CHEK1"
                     /gene_synonym="CHK1; OZEMA21"
                     /note="hexamer: AATAAA"
     polyA_site      2726
                     /gene="CHEK1"
                     /gene_synonym="CHK1; OZEMA21"
                     /note="major polyA site"
     regulatory      4099..4104
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CHEK1"
                     /gene_synonym="CHK1; OZEMA21"
                     /note="hexamer: AATAAA"
     polyA_site      4128
                     /gene="CHEK1"
                     /gene_synonym="CHK1; OZEMA21"
ORIGIN      
        1 tttggctctc cccgcctgtt ctttgcacat taaaaatgaa aaagtttgta gaactaagct
       61 aagcagatgg tcttcctgca aaaagaccgg gctgaagtaa agcattgttt tggagctggt
      121 tcacagaaaa aaggcaaaac tggttatcct gacttcaagc tccaacataa actgctcgct
      181 ttctccggga aacttgcccc gccacacaca cttgactgcg tggccagttc tttcgaagcc
      241 tctcgctccc aacacggagt tcctcccatt tcttcacagt cggctctcag cagctgctgc
      301 tggtttctcg gctccagcac cacgagtacc gcactctgag gtttacaaag cactctgctt
      361 caccgactgt gatcctcaca gtcctgtccg gtggcctcac gcaggtggcg gtgcagcctt
      421 tcaggcccag agcggccagg agcgaagccc gcagccccgc ctggaagcgc agcgcggtcg
      481 gtcgcgcgcc cctgaggctt ggaggcctgg gcttccccca gcagcgctcg agcaccgccc
      541 agtcgagcct cacaccggat gccacttcat atttgggccc agagctcaat tcgcgccgat
      601 gcggtccgcc gtccttaaat ctcttcagcc aggatctctc cccgactgca aagcagccct
      661 gggcgggagc ggcaacatct ccacgtcacc cttttggagc cgccgacatt cagaggggca
      721 ggacacggga acgcgcgctg tcttgcttta cggcgcgggt gcgcgagttt gcggcagcgt
      781 gacgccctca agttttggcg ggaaaagcgc tgcatttgga ttcctgcagt ggtgggcaaa
      841 ggacagtccg ccgaggtgct cggtggagtc atggcagtgc cctttgtgga agactgggac
      901 ttggtgcaaa ccctgggaga aggtgcctat ggagaagttc aacttgctgt gaatagagta
      961 actgaagaag cagtcgcagt gaagattgta gatatgaagc gtgccgtaga ctgtccagaa
     1021 aatattaaga aagagatctg tatcaataaa atgctaaatc atgaaaatgt agtaaaattc
     1081 tatggtcaca ggagagaagg caatatccaa tatttatttc tggagtactg tagtggagga
     1141 gagctttttg acagaataga gccagacata ggcatgcctg aaccagatgc tcagagattc
     1201 ttccatcaac tcatggcagg ggtggtttat ctgcatggta ttggaataac tcacagggat
     1261 attaaaccag aaaatcttct gttggatgaa agggataacc tcaaaatctc agactttggc
     1321 ttggcaacag tatttcggta taataatcgt gagcgtttgt tgaacaagat gtgtggtact
     1381 ttaccatatg ttgctccaga acttctgaag agaagagaat ttcatgcaga accagttgat
     1441 gtttggtcct gtggaatagt acttactgca atgctcgctg gagaattgcc atgggaccaa
     1501 cccagtgaca gctgtcagga gtattctgac tggaaagaaa aaaaaacata cctcaaccct
     1561 tggaaaaaaa tcgattctgc tcctctagct ctgctgcata aaatcttagt tgagaatcca
     1621 tcagcaagaa ttaccattcc agacatcaaa aaagatagat ggtacaacaa acccctcaag
     1681 aaaggggcaa aaaggccccg agtcacttca ggtggtgtgt cagagtctcc cagtggattt
     1741 tctaagcaca ttcaatccaa tttggacttc tctccagtaa acagtgcttc tagtgaagaa
     1801 aatgtgaagt actccagttc tcagccagaa ccccgcacag gtctttcctt atgggatacc
     1861 agcccctcat acattgataa attggtacaa gggatcagct tttcccagcc cacatgtcct
     1921 gatcatatgc ttttgaatag tcagttactt ggcaccccag gatcctcaca gaacccctgg
     1981 cagcggttgg tcaaaagaat gacacgattc tttaccaaat tggatgcaga caaatcttat
     2041 caatgcctga aagagacttg tgagaagttg ggctatcaat ggaagaaaag ttgtatgaat
     2101 caggttacta tatcaacaac tgataggaga aacaataaac tcattttcaa agtgaatttg
     2161 ttagaaatgg atgataaaat attggttgac ttccggcttt ctaagggtga tggattggag
     2221 ttcaagagac acttcctgaa gattaaaggg aagctgattg atattgtgag cagccagaag
     2281 atttggcttc ctgccacatg atcggaccat cggctctggg gaatcctggt gaatatagtg
     2341 ctgctatgtt gacattattc ttcctagaga agattatcct gtcctgcaaa ctgcaaatag
     2401 tagttcctga agtgttcact tccctgttta tccaaacatc ttccaattta ttttgtttgt
     2461 tcggcataca aataatacct atatcttaat tgtaagcaaa actttgggga aaggatgaat
     2521 agaattcatt tgattatttc ttcatgtgtg tttagtatct gaatttgaaa ctcatctggt
     2581 ggaaaccaag tttcagggga catgagtttt ccagctttta tacacacgta tctcattttt
     2641 atcaaaacat tttgtttaat tcaaaaagta catattccat gttgatttaa ttctaagatg
     2701 aaccaataaa gacataattc ttgtgacttt tggacagtag atttatcagt ctgtgaagcg
     2761 aagccagctt caaaacatat ccccaagatt tgtacttata ttttcaaaag ggcctggcca
     2821 gttatataaa cctgtttttg aattataatg attaattaaa attgcaagta ggtgtttttt
     2881 ccagtgtagt tagtaaaata cttgtatttt acagtgttgc ataaactcta gtgcttaact
     2941 aactttactc taaaaattac tgttgaacat cttaaatatt tttctatatt ttctactttc
     3001 atagccatat tttaaccttt tcaacttact ggtgaccaag cttttaggtg ataaagaata
     3061 aaagagggaa gggaagagta aggaagctat aagaaaaata gatctgattc tttgttcctt
     3121 tacctgttag acttacaaaa agtttgtttt tctaataaaa tttgtatcaa ctttggggca
     3181 tattaggttg aggccttggc tcctgcctgt agtcccagct acttaggagg ctgagagagg
     3241 aggatcgcgt gaacctggaa gtttgaggct gtagtgagct atgattgcac cagtgcactc
     3301 cagcttggat gacagagtaa gaccctacct ctaataaaaa tttttaaaat tgtaaaacat
     3361 tataaaatta atcagttatt ttaatctgaa gccaagaaca tgtagaatgt tatgattaga
     3421 gtttatcaca tattaatgta tactggcaaa ttgtgttact ggagtatacc cataggagga
     3481 ataaattcaa acctgtttta tttatttgaa cctatttacg gtatgcttaa gaattgaatc
     3541 agtataaatt ctcaaatatg ggagaaattt tgttcttgag aattatctga gtcattaata
     3601 tttttcaaaa acagctctca ctgacttgaa cctcttctgt aagctctaac cttttacctg
     3661 ctttacattt ccacttgaat gtctagtagg catctcttga ccaaaaacag cttttgattc
     3721 ctgttctcca acctgttcct ctcctagttt tctccatctc agaaatgtta cttcctctgc
     3781 aaagtctttc cctgacttat ctaaaataat aacctcctct gtttgctgtg ggaatttgta
     3841 tagaatggtg ggaaaatttc aagtttcata tttggattag ctctgacatt tatttatctg
     3901 aacactggta attgcctcag taaagacact gataataagt accttttaga gttattttaa
     3961 tctttaatgc tttaatgtgt aggaagagta tagtgtcctg ttttgcacag aaaggcattc
     4021 tgtaaataat aagttgcctt aattttcctg taatgttcat tatattgttg tgggaaggta
     4081 tttactccta ttattaaaaa taaaaatgtg taaaatttac tacctgaa
//
