LOCUS       NM_018397               7694 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens choline dehydrogenase (CHDH), mRNA; nuclear gene for
            mitochondrial product.
ACCESSION   NM_018397
VERSION     NM_018397.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7694)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   2  (bases 1 to 7694)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   3  (bases 1 to 7694)
  AUTHORS   Chang,H., Li,L., Peng,T., Grigoroiu-Serbanescu,M., Bergen,S.E.,
            Landen,M., Hultman,C.M., Forstner,A.J., Strohmaier,J., Hecker,J.,
            Schulze,T.G., Muller-Myhsok,B., Reif,A., Mitchell,P.B.,
            Martin,N.G., Cichon,S., Nothen,M.M., Jamain,S., Leboyer,M.,
            Bellivier,F., Etain,B., Kahn,J.P., Henry,C., Rietschel,M., Xiao,X.
            and Li,M.
  CONSRTM   Swedish Bipolar Study Group; MooDS Consortium
  TITLE     Identification of a Bipolar Disorder Vulnerable Gene CHDH at 3p21.1
  JOURNAL   Mol Neurobiol 54 (7), 5166-5176 (2017)
   PUBMED   27562178
  REMARK    GeneRIF: CHDH gene is located at chromosome 3p21.1, a risk region
            implicated in previous brains of bipolar disorder genome-wide
            association studies
REFERENCE   4  (bases 1 to 7694)
  AUTHORS   Jaiswal,S.K., Sukla,K.K., Chauhan,A., Lakhotia,A.R., Kumar,A. and
            Rai,A.K.
  TITLE     Choline metabolic pathway gene polymorphisms and risk for Down
            syndrome: An association study in a population with
            folate-homocysteine metabolic impairment
  JOURNAL   Eur J Clin Nutr 71 (1), 45-50 (2017)
   PUBMED   27677362
  REMARK    GeneRIF: In genotypic combination analysis considering PEMT
            -744GG/CHDH +432GG/BHMT +742GG as the reference combination, PEMT
            -744GC/CHDH +432GG/BHMT +742GG genotypic combination was
            significantly higher in mothers of a down syndrome child compared
            with that in control mothers with an odds ratio of 2.061 (95% CI:
            1.10-3.86, P=0.0342).
REFERENCE   5  (bases 1 to 7694)
  AUTHORS   Drews,K., Rozycka,A., Barlik,M., Klejewski,A., Kurzawinska,G.,
            Wolski,H., Majchrzycki,M., Gryszczynska,A., Kaminski,A. and
            Seremak-Mrozikiewicz,A.
  TITLE     Polymorphic variants of genes involved in choline pathway and the
            risk of intrauterine fetal death
  JOURNAL   Ginekol Pol 88 (4), 205-211 (2017)
   PUBMED   28509322
  REMARK    GeneRIF: There is no correlation between single CHDH rs893363 and
            CHDH rs2289205 polymorphisms and the incidence of intrauterine
            fetal death.
REFERENCE   6  (bases 1 to 7694)
  AUTHORS   Mostowska,A., Hozyasz,K.K., Wojcicki,P., Dziegelewska,M. and
            Jagodzinski,P.P.
  TITLE     Associations of folate and choline metabolism gene polymorphisms
            with orofacial clefts
  JOURNAL   J Med Genet 47 (12), 809-815 (2010)
   PUBMED   19737740
  REMARK    GeneRIF: Observational study of gene-disease association and
            gene-gene interaction. (HuGE Navigator)
REFERENCE   7  (bases 1 to 7694)
  AUTHORS   Xu,X., Gammon,M.D., Zeisel,S.H., Lee,Y.L., Wetmur,J.G.,
            Teitelbaum,S.L., Bradshaw,P.T., Neugut,A.I., Santella,R.M. and
            Chen,J.
  TITLE     Choline metabolism and risk of breast cancer in a population-based
            study
  JOURNAL   FASEB J 22 (6), 2045-2052 (2008)
   PUBMED   18230680
  REMARK    GeneRIF: single nucleotide polymorphisms of choline-metabolizing
            genes, PEMT -774G>C (rs12325817) and CHDH +432G>T (rs12676), were
            found be related to breast cancer risk
            GeneRIF: Observational study of gene-disease association and
            gene-environment interaction. (HuGE Navigator)
REFERENCE   8  (bases 1 to 7694)
  AUTHORS   Wang,Z., Dahiya,S., Provencher,H., Muir,B., Carney,E., Coser,K.,
            Shioda,T., Ma,X.J. and Sgroi,D.C.
  TITLE     The prognostic biomarkers HOXB13, IL17BR, and CHDH are regulated by
            estrogen in breast cancer
  JOURNAL   Clin Cancer Res 13 (21), 6327-6334 (2007)
   PUBMED   17975144
  REMARK    GeneRIF: HOXB13, IL17BR, and CHDH are regulated by estrogen in
            breast cancer
REFERENCE   9  (bases 1 to 7694)
  AUTHORS   da Costa,K.A., Kozyreva,O.G., Song,J., Galanko,J.A., Fischer,L.M.
            and Zeisel,S.H.
  TITLE     Common genetic polymorphisms affect the human requirement for the
            nutrient choline
  JOURNAL   FASEB J 20 (9), 1336-1344 (2006)
   PUBMED   16816108
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   10 (bases 1 to 7694)
  AUTHORS   Huang,S. and Lin,Q.
  TITLE     Functional expression and processing of rat choline dehydrogenase
            precursor
  JOURNAL   Biochem Biophys Res Commun 309 (2), 344-350 (2003)
   PUBMED   12951056
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC012467.9, CN285856.1,
            BC034502.1, AJ272267.1 and BE856786.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_018397.4.
            
            Summary: The protein encoded by this gene is a choline
            dehydrogenase that localizes to the mitochondrion. Variations in
            this gene can affect susceptibility to choline deficiency. A few
            transcript variants have been found for this gene, but the
            full-length nature of only one has been characterized to date.
            [provided by RefSeq, Dec 2010].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803611.179502.1,
                                           SRR1803611.232946.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2148874 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            MANE Ensembl match                 :: ENST00000315251.11/
                                                  ENSP00000319851.5
            RefSeq Select criteria             :: based on single
                                                  protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-30                AC012467.9         69677-69706
            31-610              CN285856.1         6-585
            611-699             BC034502.1         343-431
            700-700             AC012467.9         92320-92320
            701-1186            BC034502.1         433-918
            1187-1300           AJ272267.1         385-498
            1301-2417           BC034502.1         1033-2149
            2418-3669           AC012467.9         98485-99736
            3670-3732           BE856786.1         6-68                c
            3733-7694           AC012467.9         99800-103761
FEATURES             Location/Qualifiers
     source          1..7694
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3p21.1"
     gene            1..7694
                     /gene="CHDH"
                     /note="choline dehydrogenase"
                     /db_xref="GeneID:55349"
                     /db_xref="HGNC:HGNC:24288"
     exon            1..337
                     /gene="CHDH"
                     /inference="alignment:Splign:2.1.0"
     exon            338..408
                     /gene="CHDH"
                     /inference="alignment:Splign:2.1.0"
     exon            409..1170
                     /gene="CHDH"
                     /inference="alignment:Splign:2.1.0"
     CDS             468..2252
                     /gene="CHDH"
                     /EC_number="1.1.99.1"
                     /note="CDH; CHD"
                     /codon_start=1
                     /product="choline dehydrogenase, mitochondrial"
                     /protein_id="NP_060867.2"
                     /db_xref="CCDS:CCDS2873.1"
                     /db_xref="GeneID:55349"
                     /db_xref="HGNC:HGNC:24288"
                     /translation="MWCLLRGLGRPGALARGALGQQQSLGARALASAGSESRDEYSYV
                     VVGAGSAGCVLAGRLTEDPAERVLLLEAGPKDVLAGSKRLSWKIHMPAALVANLCDDR
                     YNWCYHTEVQRGLDGRVLYWPRGRVWGGSSSLNAMVYVRGHAEDYERWQRQGARGWDY
                     AHCLPYFRKAQGHELGASRYRGADGPLRVSRGKTNHPLHCAFLEATQQAGYPLTEDMN
                     GFQQEGFGWMDMTIHEGKRWSAACAYLHPALSRTNLKAEAETLVSRVLFEGTRAVGVE
                     YVKNGQSHRAYASKEVILSGGAINSPQLLMLSGIGNADDLKKLGIPVVCHLPGVGQNL
                     QDHLEIYIQQACTRPITLHSAQKPLRKVCIGLEWLWKFTGEGATAHLETGGFIRSQPG
                     VPHPDIQFHFLPSQVIDHGRVPTQQEAYQVHVGPMRGTSVGWLKLRSANPQDHPVIQP
                     NYLSTETDIEDFRLCVKLTREIFAQEALAPFRGKELQPGSHIQSDKEIDAFVRAKADS
                     AYHPSCTCKMGQPSDPTAVVDPQTRVLGVENLRVVDASIMPSMVSGNLNAPTIMIAEK
                     AADIIKGQPALWDKDVPVYKPRTLATQR"
     exon            1171..1322
                     /gene="CHDH"
                     /inference="alignment:Splign:2.1.0"
     exon            1323..1452
                     /gene="CHDH"
                     /inference="alignment:Splign:2.1.0"
     exon            1453..1587
                     /gene="CHDH"
                     /inference="alignment:Splign:2.1.0"
     exon            1588..1730
                     /gene="CHDH"
                     /inference="alignment:Splign:2.1.0"
     exon            1731..1833
                     /gene="CHDH"
                     /inference="alignment:Splign:2.1.0"
     exon            1834..7694
                     /gene="CHDH"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3584..3589
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CHDH"
                     /note="hexamer: AATACA"
     polyA_site      3605
                     /gene="CHDH"
                     /note="major polyA site"
     regulatory      3636..3641
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CHDH"
                     /note="hexamer: AATAAA"
     polyA_site      3669
                     /gene="CHDH"
                     /note="major polyA site"
     regulatory      6921..6926
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CHDH"
                     /note="hexamer: ATTAAA"
     polyA_site      6941
                     /gene="CHDH"
                     /note="major polyA site"
     regulatory      7674..7679
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CHDH"
                     /note="hexamer: AATAAA"
     polyA_site      7694
                     /gene="CHDH"
ORIGIN      
        1 ctcgcctgct ggggctcgga gggatgggcc gtgtccgagc gctggcggag cacgggccgc
       61 cgcggcgcgc gcctaccccc gcgcacacgt gcatgcagtg agccaggtgc atcagctgca
      121 cccgccctcg ggcttcgcgc gcctccgctg gagcccagct agcctcgcgg ctccggggcc
      181 gaccgggtcg gaactgctgc gtccccattc cggacgcaga gagcgcgcgg ggcgccggcg
      241 ccgccggtta aagtgccgcg gggcaggggc cgggccgcgg ccacccgctc ctcccgctcc
      301 ggtcccgact gtcgggctct cggccgagtc gccccggaca atcacaaaga gtgtgtaggc
      361 cagccccggt cacagagtgc accgtatcct gtcacttctg gatgtgaggg agaagtgagt
      421 catctcattc ccctccgtgg atcagaggac ttggactaga tagaagcatg tggtgtctcc
      481 tacgaggcct gggccggcct ggagccctgg cacggggagc cctggggcag cagcaatccc
      541 tgggtgcccg ggccctggcc agcgcaggct ctgagagccg ggacgagtac agctatgtgg
      601 tggtgggcgc gggctcggcg ggctgcgtgc tggctgggag gctcacggag gaccccgccg
      661 agcgcgtgct gctgctggag gccgggccca aggacgtgct cgcggggagc aagcggctct
      721 cgtggaagat ccacatgccc gcggccctgg tggccaacct gtgcgacgac aggtacaact
      781 ggtgctacca cacagaggtg cagcggggcc tggacggccg cgtgctgtac tggccacgcg
      841 gccgcgtctg gggtggctcc tcatccctca atgccatggt ctacgtccgt gggcacgccg
      901 aggactacga gcgctggcag cgccagggcg cccgcggctg ggactacgcg cactgcctgc
      961 cctacttccg caaggcgcag ggccacgagc tgggcgccag ccggtaccgg ggcgccgatg
     1021 gcccgctgcg ggtgtcccgg ggcaagacca accacccgct gcactgcgca ttcctggagg
     1081 ccacgcagca ggccggctac ccgctcaccg aggacatgaa tggcttccag caggagggct
     1141 tcggctggat ggacatgacc atccatgaag gcaaacggtg gagcgcggcc tgtgcctacc
     1201 tgcacccagc actgagccgc accaacctca aggccgaggc cgagacgctt gtgagcaggg
     1261 tgctatttga gggcacccgt gcagtgggcg tggagtatgt caagaatggc cagagccaca
     1321 gggcttatgc cagcaaggag gtgattctga gtggaggtgc catcaactct ccacagctgc
     1381 tcatgctctc tggcatcggg aatgctgatg acctcaagaa actgggcatc cctgtggtgt
     1441 gccacctacc tggggttggc cagaacctgc aagaccacct ggagatctac attcagcagg
     1501 catgcacccg ccctatcacc ctccattcag cacagaagcc cctgcggaag gtctgcattg
     1561 gtctggagtg gctctggaaa ttcacagggg agggagccac tgcccatctg gaaacaggtg
     1621 ggttcatccg cagccagcct ggggtccccc acccggacat ccagttccat ttcctgccat
     1681 cccaagtgat tgaccacggg cgggtcccca cccagcagga ggcttaccag gtacatgtgg
     1741 ggcccatgcg gggcacgagt gtgggctggc tcaaactgag aagtgccaat ccccaagacc
     1801 accctgtgat ccagcccaac tacttgtcaa cagaaactga tattgaggat ttccgtctgt
     1861 gtgtgaagct caccagagaa atttttgcac aggaagccct ggctccgttc cgagggaaag
     1921 agctccagcc aggaagccac attcagtcag ataaagagat agatgccttt gtgcgggcaa
     1981 aagccgacag cgcctaccac ccctcgtgca cctgtaagat gggccagccc tccgatccca
     2041 ctgccgtggt ggatccgcag acaagggtcc tcggggtgga aaacctcagg gtcgtcgatg
     2101 cctccatcat gcctagcatg gtcagcggca acctgaacgc ccccacaatc atgatcgcag
     2161 agaaggcagc tgacattatc aaggggcagc ctgcactctg ggacaaagat gtccctgtct
     2221 acaagcccag gacgctggcc acccagcgct aagacagttg ctgctggagg atgaccaggg
     2281 aagccccctg ataagccaag agggccagca cagcccttgc tcccaggctc ctgcctgaaa
     2341 ctatctagca cactaggacc caggtggtac cctactcagt ggctgagaat tggataaagt
     2401 cttgggaaat gagacaagta ctgggcagtg aatccagctc cttttcccca gcctttccct
     2461 gtgggccatt tggggaaggc cagcattcag cctgagatgt tcctccctgc ctcctggagg
     2521 gacagaaggg gaggatggtt aactcctgcc gcatcctttt tcttgtgttc acgtggcatt
     2581 ctctaaccca gggcagtggt tccttcccag gccatgcaca gaggctgggt gcctgccaga
     2641 cccacggagg gttcgcgaag gaaggggcat cctccttctt gagctgcaag ctttagctga
     2701 ggcagtaagt cacacagtag ttagttcagc ctgggctggc acataagtcc ccagtgtccc
     2761 tgttgagagg ggaaagttgc ctgctggttg aaaaactggc ttttcctttc tcgctgccta
     2821 atttcactct cagagtgagg caggtaactg gggctccact gggtcactct gagagggttg
     2881 tggctctggt tcttattaaa ccagggccag gtgcagggct cacacctgta atcccagcac
     2941 tttgggaagg tcacttgagc tcaggagttc aagaccagcc tgggcaacat agtgagacct
     3001 tgtctctgga aaacaattag ctgggcatgg tggtacacac ctgtagtccc agctacttgg
     3061 gaggctgagg cgggaggatg gctttagccc aggaggttga ggctcctgtg aaccctgatg
     3121 gcaccactgc actccagcct gggtgacagg gtgagaccct gtctcaaaaa aaaaaaaaaa
     3181 aaaaaaaacc tgagattttt ttggacctta ttcaagggga gaggagtctg gagggaatgc
     3241 cgcttggctt cactaccaaa ggttttatga cttagcattt taaaaggttt tccttttacc
     3301 aagaaatgca gaaaccaaga gcaaacccca cgtaccagat cactgattcc tactgaagca
     3361 gctagcccga gaaaggactc tctcatactg gtcttgggaa atgacttggc agcctgagcc
     3421 atcccagcag tggacaagga tggttaattc ttattctgta gacgtccaca ggaacagact
     3481 aggatggaaa acacagttaa gcacagccat gcaccctgtg gctgtagaaa tgcccctgtg
     3541 tgaaagaaaa ataaattcaa gactggagtt ttggaggaaa aaaaatacaa gtagcattta
     3601 caacagctct ttgctcttta aatttcttac cattcaataa agtgatccag tcactcacat
     3661 ttgaactgag atgccttctc cagactgttc tgacaaggta atgaaaataa aatgcacttg
     3721 gatttttcca gacatccaca tccaccgtca agggctggga agaaaagcat tgttgcgtgg
     3781 aaacctgcag ctatactagg cccagaataa ggacgggagg aaattggggg attccaggct
     3841 ttgctcggat ctgagaggat tggggcggct tacagactgg ggcggggggg gggggcgtcc
     3901 gacagccaca agttagtttt ctgagaaaaa ccttcgagag aggcagcagg aagaaaccca
     3961 tcacacctct ccttttaaaa atcttaagca gctcttgttt aatcttacct gtatttttta
     4021 aaatttttgt tcgaagagag ggtcaagaaa gaaaattcta aaccggccaa gagacttatt
     4081 tctcctacgg agggtcccca cctgccccac agcagcagtc catggccatc agtcacctgg
     4141 gtctcggggc tgcctgcaag agtgcagaaa caggagttca ggtgccagcc ccgtaacttg
     4201 gagggcctcg tgcagctttc ctgctggagc gtgtgtggat tcaggggcag gggatagtcc
     4261 tggaccaacc atgactgtgc tgacccttct tctctggaac aggtatggat atggcagggt
     4321 gacagtcact tagttctacc atctctgtgt cccatgggtg tgtcagtgtc tgagaccatg
     4381 gtgggttcag tctggtggct gtattttaaa ataagcaaaa cgtcatatgg taagaggagc
     4441 ccattggtga tgctgctcaa ttgcacccag gcccttcttt cactctcaaa tacttagcca
     4501 tgttctacac aatcgcacaa tttggccata aacaggaatg aggtttaaaa gaaaagatgg
     4561 taagtgtcac atgtccagaa cctgagcgtt cccctgccat gaagggagtc tttcattcac
     4621 cccaggagga gaggctgtca cctgaggaag ccagcatggg ggagaaaggc acttgctaca
     4681 aagtggggtg cacagagggt acccccccat cttcagtctc atagggaatt gggccccttg
     4741 gccaaaagct tgcagtacct aaaaacagag aaacgtgctt tgcaaaaatg gctcaaagag
     4801 cacaacagaa tgcgctgtcc atgtgcccag tgctctcagg agccctgacc tattgcagaa
     4861 gcctgtgttg aagccgctgc actagtagaa ggcagctcct gatggaatag gggatagggg
     4921 tgggctgtgt gggagccaag agcatctggc agcctgactg gagatggtga gttcctgaag
     4981 caggagttgt aggcacccaa acatctagtc tttctgcttg tagcgaaggt tgcaggcatc
     5041 atctccacga attcaaatag attcttcttg gctaggcatg gtggctcacg cctgtaatcc
     5101 cagcactttg caaggctgag gtgggtggat cacttgaggc caggagtttg agaccagcct
     5161 ggccaacatg gtgaaacccc gtctctacta aaaatacaaa aattagctgg gcatggtggt
     5221 gcacgcctgt agtcccagct acttgggagg ccgatacagg agaatcgctt gaacctgggt
     5281 ggcgcaggct gcagtgagcc aagattgcac cactgcactc caccctgggt gacagagtga
     5341 gactgtctca aaaaaaaaca acaaaaaaac agattcttct ccacccaacc atgtcccgcc
     5401 actcagaagt ggtttcatgc ttctactgat aagccaactt aacatcagat ccaatacaga
     5461 ttttttaaag ataaaatacc atctctactg gacctgttta gtggctcagg ctgccctcac
     5521 aggacatccc tgagaccacc ctgtcactct tgatgttgga accagggccc aggcctgctc
     5581 ctcattgtct cctgccctcc tagtccccag gagaggaaaa gaaatactgt tttagagaaa
     5641 taacattttc aacaaaacat ccctggagtc agattttgag ttggggtggg ctaatcaggg
     5701 agtcggggct ctctgcgtga tgtcagttct atggctaact ggtttttcta aaccagccag
     5761 ctgcctatca aaacagtaca acttttctag gaaatgcaat tggcaaagac acttacgatg
     5821 ctgagaagta cacaaggtga aactgctcca gtttttctca tagcagggtc agcaggaaag
     5881 caagtggtgc ccctggtccc atctcacaca ggtgagactg caccgagagg taacgtggcc
     5941 ctcacagccc accacgcctg gccttcgccc aattctgaaa cttcgtagga tagagctgga
     6001 aagtgccaca tggtgaagcg agatccagct gtctgggtgg atgtcggagt ccataggctg
     6061 agcagagatg gttcttagtg aggttctcgc tgccagttga cggtgaaatc atagctgcca
     6121 tttacatttt gtgagattat gaaaaacata agactaaaga aactaaatgt gttattcctg
     6181 tggacacaaa aatgtgtgtt tttcagatgg ggaggggacc aaaaaggaaa aacatttcat
     6241 cttaaaactt tcctaagaca agggaaaaca aaaaaccatg ctctacaact tcaaattttt
     6301 cttacaaaga aaaatttaat attcgatgag aggttgaacc aggcttaaag cagacatact
     6361 aggaaatggt gcagcctgta agaatgccag tttgtaagta ctgactttgg aaaagatcat
     6421 cgcctctatc agacacttag ggtcctggtc tggcaatttt ggcctgatgt gatgccacaa
     6481 gacccaacag agagagacac agagtccagg ataatgttga cagtggtgta gccctttagg
     6541 agaaatggcg ctccctgcgg ctggtattag gttaccattg gcaccgaagg aaccaggagg
     6601 ataagaatat ccataatttc agagctgccc tggcacagta cctgccctgt cggaggctct
     6661 cactggcaaa tgacagctct gtgcaaggag cactcccaag tataaaaatt attacacagt
     6721 tttattctga agaacatttt gcattttaat aaaaaaggat ttatgtcagg aaagagtcat
     6781 ttacaaacct tgaagtgttt ttgcctggat cagagtaaga atgtcttaag aagaggtttg
     6841 taaggtcttc ataacaaagt ggtgtttgtt atttacaaaa aaaaaaaaaa aaaaaaaaat
     6901 taacaggttg tctgtatact attaaaaatt ttggaccaaa attgctacct atcatctatc
     6961 tttttgattg cagggaatcc ctgccaaggt aacctgacag tcggcctaat tctgttgaca
     7021 gaaaatgaag ccttgacggt tctaattatc caaaagtggg ttttcatcag gacgtacagt
     7081 cagagtgtga gtgcattcta atgaaaactt cagccctcat tcaattgcat ataaaagccc
     7141 tcaaagagaa catacagtac agcagttttg taaaaggcaa caatacgatt tgtacagacc
     7201 ccgacactcc aatcctatag atcaccacgt tgctcctctg tccccagcac cccttatttc
     7261 ttaagcagca ccaccagtcc ccagaggaca agagcagcta tatctcatcc acctatgcat
     7321 ttctgcacca gcgatgcaaa aaacaccctg ggtgggccac agagaaaacc gtaactatgg
     7381 atacccaagg ggcagagcag ccagctggac ctgctctgta attcacttac ttacttacca
     7441 gagggtggtt caagtcaaac aaacaaaacc aaagacagtt gcaacccatg gtggatccaa
     7501 aggtacagta tcaaaatcat ggaactcttg cccaaagaaa agactgcaaa tagtcatctg
     7561 gtatatcttg tggaaagatg ataggtttat agtgactcaa aatattttag aaaaatttct
     7621 gtagtgtcaa gttctttcaa acttaaaatt ttaaccccag aggattttcg ctgaataaat
     7681 gagaattggc tcta
//
