LOCUS       NM_017780              11606 bp    mRNA    linear   PRI 09-FEB-2025
DEFINITION  Homo sapiens chromodomain helicase DNA binding protein 7 (CHD7),
            transcript variant 1, mRNA.
ACCESSION   NM_017780 XM_098762
VERSION     NM_017780.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 11606)
  AUTHORS   Driesen,J., Van Hoecke,H., Maes,L., Janssens,S., Acke,F. and De
            Leenheer,E.
  TITLE     CHD7 Disorder-Not CHARGE Syndrome-Presenting as Isolated
            Cochleovestibular Dysfunction
  JOURNAL   Genes (Basel) 15 (5), 643 (2024)
   PUBMED   38790272
  REMARK    GeneRIF: CHD7 Disorder-Not CHARGE Syndrome-Presenting as Isolated
            Cochleovestibular Dysfunction.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11606)
  AUTHORS   Gao,J., Skidmore,J.M., Cimerman,J., Ritter,K.E., Qiu,J.,
            Wilson,L.M.Q., Raphael,Y., Kwan,K.Y. and Martin,D.M.
  TITLE     CHD7 and SOX2 act in a common gene regulatory network during
            mammalian semicircular canal and cochlear development
  JOURNAL   Proc Natl Acad Sci U S A 121 (10), e2311720121 (2024)
   PUBMED   38408234
  REMARK    GeneRIF: CHD7 and SOX2 act in a common gene regulatory network
            during mammalian semicircular canal and cochlear development.
REFERENCE   3  (bases 1 to 11606)
  AUTHORS   Colin,C., Tobaruella,F.S., Correa,R.G., Sogayar,M.C. and
            Demasi,M.A.
  TITLE     Cloning and characterization of a novel alternatively spliced
            transcript of the human CHD7 putative helicase
  JOURNAL   BMC Res Notes 3, 252 (2010)
   PUBMED   20925924
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 11606)
  AUTHORS   Kallen,K., Robert,E., Mastroiacovo,P., Castilla,E.E. and Kallen,B.
  TITLE     CHARGE Association in newborns: a registry-based study
  JOURNAL   Teratology 60 (6), 334-343 (1999)
   PUBMED   10590394
REFERENCE   5  (bases 1 to 11606)
  AUTHORS   Tellier,A.L., Cormier-Daire,V., Abadie,V., Amiel,J., Sigaudy,S.,
            Bonnet,D., de Lonlay-Debeney,P., Morrisseau-Durand,M.P., Hubert,P.,
            Michel,J.L., Jan,D., Dollfus,H., Baumann,C., Labrune,P.,
            Lacombe,D., Philip,N., LeMerrer,M., Briard,M.L., Munnich,A. and
            Lyonnet,S.
  TITLE     CHARGE syndrome: report of 47 cases and review
  JOURNAL   Am J Med Genet 76 (5), 402-409 (1998)
   PUBMED   9556299
  REMARK    Review article
REFERENCE   6  (bases 1 to 11606)
  AUTHORS   Scott,D.A.
  TITLE     Esophageal Atresia / Tracheoesophageal Fistula Overview - RETIRED
            CHAPTER, FOR HISTORICAL REFERENCE ONLY
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301753
REFERENCE   7  (bases 1 to 11606)
  AUTHORS   Bardakjian,T., Weiss,A. and Schneider,A.
  TITLE     Microphthalmia/Anophthalmia/Coloboma Spectrum - RETIRED CHAPTER,
            FOR HISTORICAL REFERENCE ONLY
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301552
REFERENCE   8  (bases 1 to 11606)
  AUTHORS   Longoni,M., Pober,B.R. and High,F.A.
  TITLE     Congenital Diaphragmatic Hernia Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301533
REFERENCE   9  (bases 1 to 11606)
  AUTHORS   Balasubramanian,R. and Crowley,W.F. Jr.
  TITLE     Isolated Gonadotropin-Releasing Hormone (GnRH) Deficiency
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301509
REFERENCE   10 (bases 1 to 11606)
  AUTHORS   van Ravenswaaij-Arts,C.M., Hefner,M., Blake,K. and Martin,D.M.
  TITLE     CHD7 Disorder
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301296
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC023102.6, BC068000.1,
            BC033116.1, AK123158.1, BI039198.1, AB037837.1, BC051264.1,
            AC113143.3 and W78145.1.
            
            On Nov 22, 2018 this sequence version replaced NM_017780.3.
            
            Summary: This gene encodes a protein that contains several helicase
            family domains. Mutations in this gene have been found in some
            patients with the CHARGE syndrome. Two transcript variants encoding
            different isoforms have been found for this gene. [provided by
            RefSeq, Oct 2015].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMEA1965299,
                              SAMEA1966682 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000423902.7/ ENSP00000392028.1
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-40                AC023102.6         77931-77970
            41-2307             BC068000.1         1-2267
            2308-2442           BC033116.1         2177-2311
            2443-2680           AK123158.1         1209-1446
            2681-2907           BI039198.1         185-411
            2908-8704           AB037837.1         105-5901
            8705-10475          BC051264.1         405-2175
            10476-11225         AC113143.3         112431-113180
            11226-11606         W78145.1           9-389               c
FEATURES             Location/Qualifiers
     source          1..11606
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /map="8q12.2"
     gene            1..11606
                     /gene="CHD7"
                     /gene_synonym="CRG; HH5; IS3; KAL5"
                     /note="chromodomain helicase DNA binding protein 7"
                     /db_xref="GeneID:55636"
                     /db_xref="HGNC:HGNC:20626"
                     /db_xref="MIM:608892"
     exon            1..343
                     /gene="CHD7"
                     /gene_synonym="CRG; HH5; IS3; KAL5"
                     /inference="alignment:Splign:2.1.0"
     exon            344..2182
                     /gene="CHD7"
                     /gene_synonym="CRG; HH5; IS3; KAL5"
                     /inference="alignment:Splign:2.1.0"
     CDS             518..9511
                     /gene="CHD7"
                     /gene_synonym="CRG; HH5; IS3; KAL5"
                     /EC_number="3.6.4.12"
                     /note="isoform 1 is encoded by transcript variant 1;
                     ATP-dependent helicase CHD7; CHARGE association"
                     /codon_start=1
                     /product="chromodomain-helicase-DNA-binding protein 7
                     isoform 1"
                     /protein_id="NP_060250.2"
                     /db_xref="CCDS:CCDS47865.1"
                     /db_xref="GeneID:55636"
                     /db_xref="HGNC:HGNC:20626"
                     /db_xref="MIM:608892"
                     /translation="MADPGMMSLFGEDGNIFSEGLEGLGECGYPENPVNPMGQQMPID
                     QGFASLQPSLHHPSTNQNQTKLTHFDHYNQYEQQKMHLMDQPNRMMSNTPGNGLASPH
                     SQYHTPPVPQVPHGGSGGGQMGVYPGMQNERHGQSFVDSSSMWGPRAVQVPDQIRAPY
                     QQQQPQPQPPQPAPSGPPAQGHPQHMQQMGSYMARGDFSMQQHGQPQQRMSQFSQGQE
                     GLNQGNPFIATSGPGHLSHVPQQSPSMAPSLRHSVQQFHHHPSTALHGESVAHSPRFS
                     PNPPQQGAVRPQTLNFSSRSQTVPSPTINNSGQYSRYPYSNLNQGLVNNTGMNQNLGL
                     TNNTPMNQSVPRYPNAVGFPSNSGQGLMHQQPIHPSGSLNQMNTQTMHPSQPQGTYAS
                     PPPMSPMKAMSNPAGTPPPQVRPGSAGIPMEVGSYPNMPHPQPSHQPPGAMGIGQRNM
                     GPRNMQQSRPFIGMSSAPRELTGHMRPNGCPGVGLGDPQAIQERLIPGQQHPGQQPSF
                     QQLPTCPPLQPHPGLHHQSSPPHPHHQPWAQLHPSPQNTPQKVPVHQHSPSEPFLEKP
                     VPDMTQVSGPNAQLVKSDDYLPSIEQQPQQKKKKKKNNHIVAEDPSKGFGKDDFPGGV
                     DNQELNRNSLDGSQEEKKKKKRSKAKKDPKEPKEPKEKKEPKEPKTPKAPKIPKEPKE
                     KKAKTATPKPKSSKKSSNKKPDSEASALKKKVNKGKTEGSENSDLDKTPPPSPPPEED
                     EDPGVQKRRSSRQVKRKRYTEDLEFKISDEEADDADAAGRDSPSNTSQSEQQESVDAE
                     GPVVEKIMSSRSVKKQKESGEEVEIEEFYVKYKNFSYLHCQWASIEDLEKDKRIQQKI
                     KRFKAKQGQNKFLSEIEDELFNPDYVEVDRIMDFARSTDDRGEPVTHYLVKWCSLPYE
                     DSTWERRQDIDQAKIEEFEKLMSREPETERVERPPADDWKKSESSREYKNNNKLREYQ
                     LEGVNWLLFNWYNMRNCILADEMGLGKTIQSITFLYEIYLKGIHGPFLVIAPLSTIPN
                     WEREFRTWTELNVVVYHGSQASRRTIQLYEMYFKDPQGRVIKGSYKFHAIITTFEMIL
                     TDCPELRNIPWRCVVIDEAHRLKNRNCKLLEGLKMMDLEHKVLLTGTPLQNTVEELFS
                     LLHFLEPSRFPSETTFMQEFGDLKTEEQVQKLQAILKPMMLRRLKEDVEKNLAPKEET
                     IIEVELTNIQKKYYRAILEKNFTFLSKGGGQANVPNLLNTMMELRKCCNHPYLINGAE
                     EKILEEFKETHNAESPDFQLQAMIQAAGKLVLIDKLLPKLKAGGHRVLIFSQMVRCLD
                     ILEDYLIQRRYPYERIDGRVRGNLRQAAIDRFSKPDSDRFVFLLCTRAGGLGINLTAA
                     DTCIIFDSDWNPQNDLQAQARCHRIGQSKSVKIYRLITRNSYEREMFDKASLKLGLDK
                     AVLQSMSGRENATNGVQQLSKKEIEDLLRKGAYGALMDEEDEGSKFCEEDIDQILLRR
                     THTITIESEGKGSTFAKASFVASGNRTDISLDDPNFWQKWAKKAELDIDALNGRNNLV
                     IDTPRVRKQTRLYSAVKEDELMEFSDLESDSEEKPCAKPRRPQDKSQGYARSECFRVE
                     KNLLVYGWGRWTDILSHGRYKRQLTEQDVETICRTILVYCLNHYKGDENIKSFIWDLI
                     TPTADGQTRALVNHSGLSAPVPRGRKGKKVKAQSTQPVVQDADWLASCNPDALFQEDS
                     YKKHLKHHCNKVLLRVRMLYYLRQEVIGDQADKILEGADSSEADVWIPEPFHAEVPAD
                     WWDKEADKSLLIGVFKHGYEKYNSMRADPALCFLERVGMPDAKAIAAEQRGTDMLADG
                     GDGGEFDREDEDPEYKPTRTPFKDEIDEFANSPSEDKEESMEIHATGKHSESNAELGQ
                     LYWPNTSTLTTRLRRLITAYQRSYKRQQMRQEALMKTDRRRRRPREEVRALEAEREAI
                     ISEKRQKWTRREEADFYRVVSTFGVIFDPVKQQFDWNQFRAFARLDKKSDESLEKYFS
                     CFVAMCRRVCRMPVKPDDEPPDLSSIIEPITEERASRTLYRIELLRKIREQVLHHPQL
                     GERLKLCQPSLDLPEWWECGRHDRDLLVGAAKHGVSRTDYHILNDPELSFLDAHKNFA
                     QNRGAGNTSSLNPLAVGFVQTPPVISSAHIQDERVLEQAEGKVEEPENPAAKEKCEGK
                     EEEEETDGSGKESKQECEAEASSVKNELKGVEVGADTGSKSISEKGSEEDEEEKLEDD
                     DKSEESSQPEAGAVSRGKNFDEESNASMSTARDETRDGFYMEDGDPSVAQLLHERTFA
                     FSFWPKDRVMINRLDNICEAVLKGKWPVNRRQMFDFQGLIPGYTPTTVDSPLQKRSFA
                     ELSMVGQASISGSEDITTSPQLSKEDALNLSVPRQRRRRRRKIEIEAERAAKRRNLME
                     MVAQLRESQVVSENGQEKVVDLSKASREATSSTSNFSSLSSKFILPNVSTPVSDAFKT
                     QMELLQAGLSRTPTRHLLNGSLVDGEPPMKRRRGRRKNVEGLDLLFMSHKRTSLSAED
                     AEVTKAFEEDIETPPTRNIPSPGQLDPDTRIPVINLEDGTRLVGEDAPKNKDLVEWLK
                     LHPTYTVDMPSYVPKNADVLFSSFQKPKQKRHRCRNPNKLDINTLTGEERVPVVNKRN
                     GKKMGGAMAPPMKDLPRWLEENPEFAVAPDWTDIVKQSGFVPESMFDRLLTGPVVRGE
                     GASRRGRRPKSEIARAAAAAAAVASTSGINPLLVNSLFAGMDLTSLQNLQNLQSLQLA
                     GLMGFPPGLATAATAGGDAKNPAAVLPLMLPGMAGLPNVFGLGGLLNNPLSAATGNTT
                     TASSQGEPEDSTSKGEEKGNENEDENKDSEKSTDAVSAADSANGSVGAATAPAGLPSN
                     PLAFNPFLLSTMAPGLFYPSMFLPPGLGGLTLPGFPALAGLQNAVGSSEEKAADKAEG
                     GPFKDGETLEGSDAEESLDKTAESSLLEDEIAQGEELDSLDGGDEIENNENDE"
     exon            2183..2613
                     /gene="CHD7"
                     /gene_synonym="CRG; HH5; IS3; KAL5"
                     /inference="alignment:Splign:2.1.0"
     exon            2614..2755
                     /gene="CHD7"
                     /gene_synonym="CRG; HH5; IS3; KAL5"
                     /inference="alignment:Splign:2.1.0"
     exon            2756..2893
                     /gene="CHD7"
                     /gene_synonym="CRG; HH5; IS3; KAL5"
                     /inference="alignment:Splign:2.1.0"
     exon            2894..2959
                     /gene="CHD7"
                     /gene_synonym="CRG; HH5; IS3; KAL5"
                     /inference="alignment:Splign:2.1.0"
     exon            2960..3015
                     /gene="CHD7"
                     /gene_synonym="CRG; HH5; IS3; KAL5"
                     /inference="alignment:Splign:2.1.0"
     exon            3016..3130
                     /gene="CHD7"
                     /gene_synonym="CRG; HH5; IS3; KAL5"
                     /inference="alignment:Splign:2.1.0"
     exon            3131..3214
                     /gene="CHD7"
                     /gene_synonym="CRG; HH5; IS3; KAL5"
                     /inference="alignment:Splign:2.1.0"
     exon            3215..3352
                     /gene="CHD7"
                     /gene_synonym="CRG; HH5; IS3; KAL5"
                     /inference="alignment:Splign:2.1.0"
     exon            3353..3474
                     /gene="CHD7"
                     /gene_synonym="CRG; HH5; IS3; KAL5"
                     /inference="alignment:Splign:2.1.0"
     exon            3475..3718
                     /gene="CHD7"
                     /gene_synonym="CRG; HH5; IS3; KAL5"
                     /inference="alignment:Splign:2.1.0"
     exon            3719..3895
                     /gene="CHD7"
                     /gene_synonym="CRG; HH5; IS3; KAL5"
                     /inference="alignment:Splign:2.1.0"
     exon            3896..4039
                     /gene="CHD7"
                     /gene_synonym="CRG; HH5; IS3; KAL5"
                     /inference="alignment:Splign:2.1.0"
     exon            4040..4295
                     /gene="CHD7"
                     /gene_synonym="CRG; HH5; IS3; KAL5"
                     /inference="alignment:Splign:2.1.0"
     exon            4296..4506
                     /gene="CHD7"
                     /gene_synonym="CRG; HH5; IS3; KAL5"
                     /inference="alignment:Splign:2.1.0"
     exon            4507..4702
                     /gene="CHD7"
                     /gene_synonym="CRG; HH5; IS3; KAL5"
                     /inference="alignment:Splign:2.1.0"
     exon            4703..4870
                     /gene="CHD7"
                     /gene_synonym="CRG; HH5; IS3; KAL5"
                     /inference="alignment:Splign:2.1.0"
     exon            4871..5050
                     /gene="CHD7"
                     /gene_synonym="CRG; HH5; IS3; KAL5"
                     /inference="alignment:Splign:2.1.0"
     exon            5051..5161
                     /gene="CHD7"
                     /gene_synonym="CRG; HH5; IS3; KAL5"
                     /inference="alignment:Splign:2.1.0"
     exon            5162..5367
                     /gene="CHD7"
                     /gene_synonym="CRG; HH5; IS3; KAL5"
                     /inference="alignment:Splign:2.1.0"
     exon            5368..5567
                     /gene="CHD7"
                     /gene_synonym="CRG; HH5; IS3; KAL5"
                     /inference="alignment:Splign:2.1.0"
     exon            5568..5727
                     /gene="CHD7"
                     /gene_synonym="CRG; HH5; IS3; KAL5"
                     /inference="alignment:Splign:2.1.0"
     exon            5728..5817
                     /gene="CHD7"
                     /gene_synonym="CRG; HH5; IS3; KAL5"
                     /inference="alignment:Splign:2.1.0"
     exon            5818..5921
                     /gene="CHD7"
                     /gene_synonym="CRG; HH5; IS3; KAL5"
                     /inference="alignment:Splign:2.1.0"
     exon            5922..6051
                     /gene="CHD7"
                     /gene_synonym="CRG; HH5; IS3; KAL5"
                     /inference="alignment:Splign:2.1.0"
     exon            6052..6124
                     /gene="CHD7"
                     /gene_synonym="CRG; HH5; IS3; KAL5"
                     /inference="alignment:Splign:2.1.0"
     exon            6125..6182
                     /gene="CHD7"
                     /gene_synonym="CRG; HH5; IS3; KAL5"
                     /inference="alignment:Splign:2.1.0"
     exon            6183..6411
                     /gene="CHD7"
                     /gene_synonym="CRG; HH5; IS3; KAL5"
                     /inference="alignment:Splign:2.1.0"
     exon            6412..6620
                     /gene="CHD7"
                     /gene_synonym="CRG; HH5; IS3; KAL5"
                     /inference="alignment:Splign:2.1.0"
     exon            6621..7292
                     /gene="CHD7"
                     /gene_synonym="CRG; HH5; IS3; KAL5"
                     /inference="alignment:Splign:2.1.0"
     exon            7293..7453
                     /gene="CHD7"
                     /gene_synonym="CRG; HH5; IS3; KAL5"
                     /inference="alignment:Splign:2.1.0"
     exon            7454..7681
                     /gene="CHD7"
                     /gene_synonym="CRG; HH5; IS3; KAL5"
                     /inference="alignment:Splign:2.1.0"
     exon            7682..8125
                     /gene="CHD7"
                     /gene_synonym="CRG; HH5; IS3; KAL5"
                     /inference="alignment:Splign:2.1.0"
     exon            8126..8347
                     /gene="CHD7"
                     /gene_synonym="CRG; HH5; IS3; KAL5"
                     /inference="alignment:Splign:2.1.0"
     exon            8348..8488
                     /gene="CHD7"
                     /gene_synonym="CRG; HH5; IS3; KAL5"
                     /inference="alignment:Splign:2.1.0"
     exon            8489..8593
                     /gene="CHD7"
                     /gene_synonym="CRG; HH5; IS3; KAL5"
                     /inference="alignment:Splign:2.1.0"
     exon            8594..11606
                     /gene="CHD7"
                     /gene_synonym="CRG; HH5; IS3; KAL5"
                     /inference="alignment:Splign:2.1.0"
     regulatory      10452..10457
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CHD7"
                     /gene_synonym="CRG; HH5; IS3; KAL5"
                     /note="hexamer: AATAAA"
     polyA_site      10475
                     /gene="CHD7"
                     /gene_synonym="CRG; HH5; IS3; KAL5"
                     /note="major polyA site"
     regulatory      10484..10489
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CHD7"
                     /gene_synonym="CRG; HH5; IS3; KAL5"
                     /note="hexamer: ATTATA"
     polyA_site      10509
                     /gene="CHD7"
                     /gene_synonym="CRG; HH5; IS3; KAL5"
     regulatory      10551..10556
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CHD7"
                     /gene_synonym="CRG; HH5; IS3; KAL5"
                     /note="hexamer: AATACA"
     polyA_site      10559
                     /gene="CHD7"
                     /gene_synonym="CRG; HH5; IS3; KAL5"
     regulatory      11585..11590
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CHD7"
                     /gene_synonym="CRG; HH5; IS3; KAL5"
                     /note="hexamer: ATTAAA"
     polyA_site      11606
                     /gene="CHD7"
                     /gene_synonym="CRG; HH5; IS3; KAL5"
ORIGIN      
        1 gcgcaggcgc tggcgtgctg gggccgcggc ggcggcggcg gcggcggcgg cagcggcggc
       61 ggcggcggcg gcgcgggggt tgagtcgtgg tggtgcggac gcgctcgtgc tcgggaacta
      121 tcggattaaa cttgaatcga gtgaaattac acaaaggagc gccgcggagg aggcggcccg
      181 gggacccgga caccctgaaa ctcaccagag acccgttcgc ccccggccaa ctccgtgccc
      241 gtggattcag ccccctggcc gcagctgccg agccaactcc ggagcccgct ctgcgttttg
      301 ttttcccctc ggcactaggc agcggaggag cccgaccgac ccggacctat atccagactt
      361 tgcctgacac tgcagggtcc aagagaatta aagaaatatg gaatgacatg aagaagatta
      421 gttaaggatt ataggctttg agggcaaaca cctcagtgaa gtgaagcaca ggcaagctcc
      481 tgagctgtgg tttggaggag ccgtgtgttg gaagaagatg gcagatccag gaatgatgag
      541 tctttttggc gaggatggga atattttcag tgaaggtctt gaaggcctcg gagaatgtgg
      601 ttacccggaa aatccagtaa atcctatggg tcagcaaatg ccaatagacc aaggctttgc
      661 ctctttacag ccatcccttc atcatccttc aactaatcaa aatcaaacaa agctgacaca
      721 ttttgatcac tataatcagt atgaacaaca aaagatgcat ctgatggatc agccgaacag
      781 aatgatgagc aacacccctg ggaacggact cgcgtctccg cactcgcagt atcacacccc
      841 tcccgttcct caggtgcccc atggtggcag tggtggcggt cagatgggtg tctaccctgg
      901 catgcagaat gagaggcatg ggcaatcctt tgtggacagc agctccatgt ggggccccag
      961 ggctgttcag gtaccagacc agatacgagc cccctaccag cagcagcagc cacagccgca
     1021 gccaccgcag ccggctccgt cggggccccc tgcacagggc caccctcagc acatgcagca
     1081 gatgggcagc tatatggcac gtggggattt ttccatgcag cagcatggtc agccacagca
     1141 gaggatgagc cagttttccc aaggccaaga gggcctcaat cagggaaatc cttttattgc
     1201 cacctcagga cctggccact tgtcccacgt gccccagcag agtcccagca tggcaccttc
     1261 cttgcgtcac tcggtgcagc agttccatca ccacccctct actgctctcc atggagaatc
     1321 cgttgcccac agtcccagat tctccccgaa tcctccccaa caaggggctg ttaggccgca
     1381 aacccttaac tttagttctc ggagccagac agtcccctct cctactataa acaactcagg
     1441 gcagtattct cgatatcctt acagtaacct aaatcaggga ttagttaaca atacagggat
     1501 gaatcaaaat ttaggcctta caaataatac tccaatgaat cagtccgtac caagataccc
     1561 caatgctgta ggattcccat caaacagtgg tcaaggacta atgcaccagc agcccatcca
     1621 ccccagtggc tcacttaacc aaatgaacac acaaactatg catccttcac agcctcaggg
     1681 aacttatgcc tctccacctc ccatgtcacc catgaaagca atgagtaatc cagcaggcac
     1741 tcctcctcca caagtcaggc cgggaagtgc tgggatacca atggaagttg gcagttatcc
     1801 aaatatgccc catcctcagc catctcacca gccccctggt gccatgggaa tcggacagag
     1861 gaatatgggc cccagaaaca tgcagcagtc tcgtccattt ataggcatgt cctcggcacc
     1921 aagggaattg actgggcaca tgaggccaaa tggttgtcct ggtgttggcc ttggagaccc
     1981 acaagcaatc caggaacgac tgatacctgg ccaacaacat cctggtcaac agccatcttt
     2041 tcagcagttg ccaacctgtc ctccactgca gcctcacccg ggcttgcacc accagtcttc
     2101 acctccacac cctcatcacc agccttgggc acagctccac ccatcacccc agaacacccc
     2161 gcagaaagtg cctgtgcatc agcattcccc gtcggagccc tttctagaga aaccagtgcc
     2221 ggatatgact caggttagtg gaccgaatgc tcagctagtg aagagtgatg attacctgcc
     2281 atcaatagaa cagcagccac aacaaaagaa gaagaaaaag aaaaacaacc acattgtagc
     2341 agaggatccc agtaaaggtt ttggtaaaga tgacttccct ggtggggtag ataaccaaga
     2401 actaaatagg aactcactgg atgggtccca agaagaaaaa aagaaaaaga aaaggtcaaa
     2461 ggcaaaaaaa gacccgaagg aaccgaaaga acccaaggag aaaaaagagc ccaaggaacc
     2521 caagaccccg aaagccccta agattcccaa agagccaaag gaaaagaaag caaaaactgc
     2581 cacgccaaaa cccaaatcca gcaaaaagtc aagtaataag aaacctgact cagaagcaag
     2641 tgctttgaag aaaaaggtca acaagggaaa aacagaaggt tctgaaaatt cagacttaga
     2701 caaaacaccc ccaccatctc ctcctcctga agaagatgag gacccaggtg ttcagaagag
     2761 acggtccagc agacaggtga agagaaagcg ctacactgaa gacctggagt tcaagatttc
     2821 tgatgaggag gcagatgatg cagatgctgc tgggagggat tccccctcca acacctccca
     2881 gtcagaacag caggaatctg ttgatgcaga aggcccagtg gtagaaaaaa ttatgagcag
     2941 tcgttcagta aaaaagcaga aggaatctgg agaggaggta gaaattgagg aattctatgt
     3001 gaaatacaaa aacttctctt atcttcattg tcagtgggca tctatagaag atctggaaaa
     3061 agataagaga attcagcaaa aaattaaacg atttaaggca aagcagggcc agaacaagtt
     3121 cctttcagag attgaggatg agctttttaa tccagattat gtggaggttg accggataat
     3181 ggactttgca cgtagcacag atgaccgggg agagcctgtg actcactatc tggtgaagtg
     3241 gtgttcactt ccttatgaag acagcacgtg ggagcggagg caggacatag atcaagcaaa
     3301 gatcgaggag tttgagaaac taatgtccag ggagccggaa acagagcgtg tggagcgacc
     3361 tcctgctgat gattggaaga aatcggagag ttccagggag tataaaaaca ataacaaact
     3421 cagggaatac cagttggagg gagtaaactg gctacttttc aattggtaca acatgcgaaa
     3481 ctgcatttta gcagatgaaa tgggtttggg aaaaactatc cagtccatta catttctcta
     3541 tgagatatat ttgaaaggaa tccatggccc ttttttagta attgccccat tgtccacaat
     3601 ccccaactgg gaaagggaat tccgaacctg gacagagttg aacgtggttg tgtatcatgg
     3661 gagtcaagct agtcgtcgga ccattcagtt gtatgaaatg tacttcaaag atccccaggg
     3721 tcgagtgata aaggggtcct ataagtttca tgccatcatc actacatttg agatgatttt
     3781 gactgattgt cctgagctgc ggaatattcc atggcgctgt gtagtcattg atgaagccca
     3841 caggctgaag aacaggaact gcaagctgtt ggagggactc aagatgatgg acttggaaca
     3901 caaagtgctg ctgacgggaa ccccactcca gaacactgtg gaagaactct tcagcttgct
     3961 tcatttcttg gaaccaagtc gcttcccttc agaaaccaca tttatgcaag aatttggtga
     4021 tctaaaaaca gaagagcagg tgcaaaaact tcaagctatt ctaaagccaa tgatgttgag
     4081 acgtctcaaa gaggatgtag aaaagaactt ggcccccaaa gaagaaacta ttattgaagt
     4141 tgagctaaca aacattcaga agaaatatta ccgagccatc cttgagaaga atttcacatt
     4201 tctttccaaa ggcggtggtc aagctaacgt acctaaccta ttaaacacta tgatggaatt
     4261 gcggaagtgc tgcaatcatc cgtaccttat caatggtgct gaagagaaaa ttttggaaga
     4321 gtttaaagaa acacacaatg cagagtctcc agattttcag ctccaggcaa tgatccaggc
     4381 tgctggcaag ctagtgctga ttgacaagct gctgccaaaa ctgaaggctg gtggccacag
     4441 ggtgcttatc ttttcccaga tggtgcgctg cttggacata ctggaagact acctcattca
     4501 aagacggtac ccatatgaaa ggatcgacgg ccgagtaaga ggcaacctcc gccaggcagc
     4561 tatcgacaga ttctccaaac ctgattctga taggtttgtt ttcctcctgt gtacaagggc
     4621 aggaggttta ggcattaacc tcactgctgc tgatacctgc atcatctttg attcagactg
     4681 gaatccccaa aatgacctcc aggctcaggc tagatgtcat agaataggac agagcaaatc
     4741 tgtgaaaatc tacaggctga ttacaagaaa ttcctatgaa agggaaatgt tcgacaaggc
     4801 tagtttgaaa ctgggcctgg ataaagctgt gctacagtct atgagtggaa gagaaaatgc
     4861 taccaatggg gtacaacagc tttccaagaa agaaatagag gatcttctac gaaaaggggc
     4921 ctatggtgca ctcatggatg aggaggatga agggtctaaa ttctgtgaag aagatattga
     4981 tcagatcctc ctacgtcgaa cccacaccat taccattgag tcagaaggga aaggttccac
     5041 atttgctaag gccagttttg ttgcatctgg aaataggaca gatatttcct tggatgatcc
     5101 aaatttctgg caaaagtggg ctaagaaggc tgaattggat attgatgcct taaatgggag
     5161 gaacaacctg gttattgata ctccaagagt gagaaagcag accaggctct acagtgcagt
     5221 gaaggaagat gagctgatgg agttctcaga cttggaaagt gattctgaag aaaagccctg
     5281 tgcaaagcca cggcgtcccc aggataagtc acagggctat gcaaggagtg aatgtttcag
     5341 ggtggagaag aatctgcttg tctatggttg gggacggtgg acagacattc tttcccacgg
     5401 acgctataaa cgccaactca ctgagcaaga tgtagaaacc atctgcagaa ccatcctggt
     5461 gtactgtctt aatcattaca aaggggatga gaatatcaaa agcttcatct gggatctgat
     5521 cacacccaca gcggatggcc agactcgagc cttggtcaac cattccggtt tgtcagctcc
     5581 tgtgccaagg ggaaggaagg gaaagaaggt gaaagcccag agcacacagc cggtggtgca
     5641 ggatgccgac tggctggcca gctgcaaccc agatgccctg ttccaggagg acagctacaa
     5701 gaaacacctg aagcatcact gtaacaaggt cctgctgcgt gtccgcatgc tgtactacct
     5761 aagacaagaa gtgataggag accaggcgga taagatctta gagggtgctg actcaagtga
     5821 agccgatgtg tggatccctg aacctttcca tgctgaagtt cctgcagatt ggtgggataa
     5881 ggaagcagac aaatccctct taattggagt gttcaaacat ggctatgaga agtacaactc
     5941 catgcgagct gaccccgcgc tgtgctttct ggaacgagtc ggtatgcctg atgccaaggc
     6001 catagctgcc gagcaaagag gaacagacat gctagcagat ggtggtgacg ggggagaatt
     6061 tgatagagaa gatgaagacc cagaatataa accaaccaga acaccgttca aagatgaaat
     6121 agatgaattt gcaaattctc cttcagagga taaggaagaa tccatggaaa tacatgccac
     6181 aggcaagcac agtgagagta atgctgagtt aggccaactt tactggccta acacttcaac
     6241 cctgactaca cgtctgcgcc ggctcattac tgcctatcag cgcagctata aaaggcaaca
     6301 gatgaggcaa gaggccctaa tgaagactga ccggcgcaga cggcggcctc gagaggaagt
     6361 gagagctctg gaagcggaaa gggaagctat tatatctgag aagcggcaaa agtggacaag
     6421 aagagaagag gctgattttt accgtgtggt atccaccttt ggggttattt ttgaccctgt
     6481 gaaacagcaa tttgactgga accaatttag agcctttgcc aggcttgaca aaaaatctga
     6541 tgagagtttg gagaaatact tcagttgttt tgtggccatg tgtaggcgag tatgtcgaat
     6601 gcccgtcaag ccagatgatg aaccgcccga cctctcctcc ataattgagc cgatcacaga
     6661 ggagcgagcc tctcgaactc tgtaccgcat tgagctgcta cggaagatcc gcgagcaggt
     6721 tctccatcac ccccagctgg gagagaggct taagctctgc cagccaagct tggatctgcc
     6781 agagtggtgg gagtgtggac ggcatgaccg agacttgctg gttggtgctg ctaaacacgg
     6841 ggtcagtcgg acggattatc acatcctcaa tgaccctgag ttatccttct tggatgcaca
     6901 taaaaacttt gctcaaaaca gaggggcagg taatacatct tccttgaacc cactggcagt
     6961 tggatttgtc cagactcctc cagtcatctc atctgctcat attcaagatg agagggtact
     7021 ggaacaagcc gaaggcaaag tggaggagcc tgaaaaccca gctgccaagg agaaatgtga
     7081 gggcaaagaa gaggaagaag aaaccgatgg cagcgggaag gagagcaagc aggaatgtga
     7141 ggcagaggcc agctctgtga aaaatgaact gaaaggtgtt gaggtcggcg cagacactgg
     7201 gtccaaatct atttcagaga aaggttccga agaggatgaa gaggaaaagc tggaggatga
     7261 cgataagtcg gaagagtctt cccagcccga agcaggagct gtctctagag ggaagaattt
     7321 tgatgaagaa agcaatgctt ccatgagcac tgctagagat gaaacccgag atggattcta
     7381 catggaggac ggagatcctt cagtagctca gctccttcat gaaagaacat ttgccttctc
     7441 gttttggcct aaggatagag taatgataaa ccgcttagac aacatctgtg aagcagtgtt
     7501 gaaaggcaaa tggccagtaa ataggcgcca gatgtttgat ttccaaggcc tcatcccagg
     7561 ttacacaccc accacagtgg acagcccctt gcagaagagg agctttgctg agctctccat
     7621 ggtcggccaa gccagcatta gtgggagtga ggacatcact acgtctcctc agttgtcaaa
     7681 ggaagatgcc ctcaacctct ctgtccctcg ccagcggagg aggaggagga gaaaaatcga
     7741 aattgaggcc gaaagagctg ccaagaggcg aaatctcatg gagatggttg cccagcttcg
     7801 agagtctcag gtggtctcag aaaatggaca agaaaaagtt gtagatttat caaaggcctc
     7861 aagagaggca acaagctcta cctcaaattt ttcatctctt tcttcaaagt ttatcttgcc
     7921 taatgtctca acaccagtgt ctgatgcctt taagactcaa atggaactgc tccaagcagg
     7981 cctttcgcgc acacccacaa ggcatctcct taatggctcc ctagtggatg gagagcctcc
     8041 catgaagagg aggcggggaa ggaggaaaaa tgtggaggga cttgatctgc ttttcatgag
     8101 ccacaaacgg acgtcattga gtgcagagga tgctgaggtg accaaagctt ttgaagaaga
     8161 tatagagacc ccaccaacaa gaaacattcc ttctcccgga cagctggacc cagacacacg
     8221 gatccctgtt atcaatcttg aagatgggac taggctggtg ggggaagatg ctcctaaaaa
     8281 taaggattta gttgaatggc tgaagctgca ccctacttac actgttgata tgccaagtta
     8341 tgtaccaaag aatgcagatg tgctgttttc ctcatttcag aaaccgaaac agaaacgaca
     8401 tagatgtcga aaccctaata aattggatat aaacactttg acaggagaag aaagggtgcc
     8461 tgttgtcaat aaacgaaatg ggaagaagat gggtggagct atggcgcctc caatgaagga
     8521 tctacccagg tggctggaag aaaatcctga atttgcagtt gctccagact ggactgatat
     8581 agttaagcag tctggttttg ttcctgagtc gatgtttgac cgccttctca ctgggcctgt
     8641 agtgcgggga gagggagcga gcagaagagg aagaaggccc aaaagtgaga tcgccagagc
     8701 agccgcggcc gccgctgctg tggcctccac gtcagggatc aaccctttgc tggtgaacag
     8761 cctgtttgct ggaatggacc tgacgagcct tcagaatctc cagaatctcc agtcgctcca
     8821 gctggcaggc ctcatgggct tccctccagg actggcaaca gctgccaccg ccggaggcga
     8881 tgcgaagaac cctgctgctg tgctgcccct gatgctgcca ggaatggcgg gcctgcccaa
     8941 cgtgtttggc ttgggcgggc tgttgaataa ccctctgtca gctgctactg gaaacaccac
     9001 tactgcttct agtcaaggag aaccggaaga cagcacttca aaaggagagg agaaaggaaa
     9061 tgagaatgaa gacgagaaca aagactctga gaaaagcaca gatgctgttt cggctgctga
     9121 ctctgcgaat ggatctgttg gtgctgctac tgccccggct ggattgccct caaacccgct
     9181 agccttcaac cctttcctcc tgtccacaat ggccccgggc ctcttctacc catccatgtt
     9241 tctacctcca ggactggggg gattgacgct gcctgggttc ccagcattgg caggacttca
     9301 gaatgccgtg ggctccagcg aagaaaaggc tgctgacaag gctgagggag gaccctttaa
     9361 agatggagag acccttgaag gcagcgatgc cgaggagagc ctggataaga ctgcagagtc
     9421 ctccctctta gaagacgaaa tagcacaggg tgaagagcta gactcacttg atggggggga
     9481 tgaaatagaa aacaatgaaa atgatgaata accagtacca gttccagttc aagtgtttaa
     9541 aacttttgac aagtggtagt cctactgttt acactcacag ttaatgttca tacctagttt
     9601 tataagctgt tctgtaacat agtgtagcaa aaaaaaaagt tcaagtcatg ttatacaggt
     9661 gtgtcaaaag gtatcttggt cattaagtat tgtgcagtgc attatttatt atccctagga
     9721 gagatgaaat ttgagaggtg atcatgtctt tttaaggaaa cttacataat gctctgcttt
     9781 ttttttttct cttggtacca ttggtattat aataaagagc aatttgtaac tgagtggcac
     9841 taatggaaga aagtgctgct caaaggaagt atgaagttat atatttaatt ttttaatttt
     9901 aatttttaat ttttttgctg tgaaggtcaa gctgaaattt accatacata tcatacttgc
     9961 tcatttgttt ccctttttga ctgtatgggg gttcccacac tcgtgcatac acacacatcc
    10021 atacactctg acaatctcca cgctagtgtg aacgcctctg tcccgaggcg cagcaataat
    10081 aaggcagctg ttgaatgtga agggtccctt tggaaaatta acctactggg agggttcttg
    10141 ccagacagaa ctacagttcc attgtctcgt ggtcttgtaa tgcactggta aaaacaaaat
    10201 aaatagatga ataaataaag agtgagagaa gagagaatca ggtacctttt ttaaattaaa
    10261 ggactttgtt actttagcca caaagctaaa acagcattac ctcagctcta aactagcctt
    10321 gaagtttaca gacatgactt tgtaaatgta ttgtttttct ttgttgtgat gtccttttat
    10381 ttttttcttt gaaaactgct atcatgtaag ataaaatgta aattgctgcc aactgtagta
    10441 atgatgcttt taataaaagt gacccatgat atgcagagat gtaattatag aatgtagtat
    10501 atagggaaac tggtgttcac tctatttttt ttgtattcgc aatagatgca aatacagcat
    10561 tctggctttt gtacagtttc ttgatatatc ctcttatact agattagctt ttggtaagac
    10621 actaaactgt ctcgaagact agacaggaag gaaaaccttg aattactctc acataattcc
    10681 actccagata tctcaacaga aatgcataca aaaagctcct attactccct caaaagggca
    10741 tctgagaccg agaatactta gaaatgtgtg cagcgtgtga taatgtggta cactgaagaa
    10801 caaaagggca aaagaaaaat gaggctttaa caggcacaat atctaggtca tttatccttg
    10861 gttaatgggt agaaaaacac aatgcggtag tgtcagcaag ggacacaaag gcactctggt
    10921 gtcctgcaga ccagcgctcg atgccagaaa ccagtgtgtg gaaaaaccca tgtggaattg
    10981 aaacagaccc acttaagcac gcacgcgcgc acgcacggtc tcaggagcta ctgatttgtg
    11041 gacccctttt tgacctttgg tatttaaagt aaaatataat ttgagatcta ctgttttcac
    11101 ctttttatgt cacctgaacc aacacaaagc catatttcca tccagttaaa aagcagggga
    11161 agggatgtgg acgagagtgt ttcgtgtgtg ttgccttcct ccacaccctt cccccaggac
    11221 gtccgcacac agtccacctc cagccagtgt gctgccagac aggtggtcta aattcctccc
    11281 agactgtgag attcagttcg tttatgcccc aagatgaaaa tatgcccctt aatgattctg
    11341 ggaaagaaac aacctgaacc ctccacctta cagatcctgt gattgctttt atttatcatc
    11401 gtcgttgtct gctgaaagtg aatgggccgt gcacactgga ggaaagtgcc ttgaagagaa
    11461 tattttttga aagggaatta tttgaacacg ggaaagtgaa actaggtctg catgaagtat
    11521 aggaaattta agtatttaag taacaaagat gttagcaggg agaatttgct taataaaatg
    11581 agtcattaaa gggtgttttt tttaaa
//
